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Hello! This is an automated update of the following workflow: workflows/microbiome/metagenomic-genes-catalogue. I created this PR because I think one or more of the component tools are out of date, i.e. there is a newer version available on the ToolShed.
By comparing with the latest versions available on the ToolShed, it seems the following tools are outdated:
toolshed.g2.bx.psu.edu/repos/iuc/amrfinderplus/amrfinderplus/3.12.8+galaxy0 should be updated to toolshed.g2.bx.psu.edu/repos/iuc/amrfinderplus/amrfinderplus/4.2.7+galaxy0
toolshed.g2.bx.psu.edu/repos/iuc/coverm_contig/coverm_contig/0.8.0+galaxy0 should be updated to toolshed.g2.bx.psu.edu/repos/iuc/coverm_contig/coverm_contig/0.8.0+galaxy1
toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.35+galaxy1 should be updated to toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.35+galaxy2
The workflow release number has been updated from 1.3 to 1.4.
If you want to skip this change, close this PR without deleting the branch. It will be reopened if another change is detected.
Any commit from another author than 'planemo-autoupdate' will prevent more auto-updates.
To ignore manual changes and allow autoupdates, delete the branch.
2026-08-03 09:47:39 - MEGAHIT v1.2.92026-08-03 09:47:39 - Using megahit_core with POPCNT and BMI2 support2026-08-03 09:47:39 - Convert reads to binary library2026-08-03 09:47:40 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 0 (/tmp/tmpy96jvlqm/files/8/9/5/dataset_89502fc5-67c8-40c6-82bf-e0370d0a8cf7.dat,/tmp/tmpy96jvlqm/files/5/4/a/dataset_54a460a2-e6ce-4524-9b4e-98bcf19898f8.dat): pe, 500000 reads, 101 max length'2026-08-03 09:47:40 - b'INFO utils/utils.h : 152 - Real: 0.6523\tuser: 0.6180\tsys: 0.0350\tmaxrss: 29776'2026-08-03 09:47:40 - Start assembly. Number of CPU threads 1 2026-08-03 09:47:40 - k list: 21,29,39,59,79,99,119,141 2026-08-03 09:47:40 - Memory used: 150936809472026-08-03 09:47:40 - Extract solid (k+1)-mers for k = 21 2026-08-03 09:47:48 - Build graph for k = 21 2026-08-03 09:47:56 - Assemble contigs from SdBG for k = 212026-08-03 09:48:21 - Local assembly for k = 212026-08-03 09:48:24 - Extract iterative edges from k = 21 to 29 2026-08-03 09:48:26 - Build graph for k = 29 2026-08-03 09:48:29 - Assemble contigs from SdBG for k = 292026-08-03 09:48:46 - Local assembly for k = 292026-08-03 09:48:49 - Extract iterative edges from k = 29 to 39 2026-08-03 09:48:50 - Build graph for k = 39 2026-08-03 09:48:52 - Assemble contigs from SdBG for k = 392026-08-03 09:49:04 - Local assembly for k = 392026-08-03 09:49:08 - Extract iterative edges from k = 39 to 59 2026-08-03 09:49:09 - Build graph for k = 59 2026-08-03 09:49:10 - Assemble contigs from SdBG for k = 592026-08-03 09:49:18 - Local assembly for k = 592026-08-03 09:49:21 - Extract iterative edges from k = 59 to 79 2026-08-03 09:49:21 - Build graph for k = 79 2026-08-03 09:49:22 - Assemble contigs from SdBG for k = 792026-08-03 09:49:28 - Local assembly for k = 792026-08-03 09:49:30 - Extract iterative edges from k = 79 to 99 2026-08-03 09:49:31 - Build graph for k = 99 2026-08-03 09:49:32 - Assemble contigs from SdBG for k = 992026-08-03 09:49:36 - Local assembly for k = 992026-08-03 09:49:38 - Extract iterative edges from k = 99 to 119 2026-08-03 09:49:38 - Build graph for k = 119 2026-08-03 09:49:39 - Assemble contigs from SdBG for k = 1192026-08-03 09:49:42 - Local assembly for k = 1192026-08-03 09:49:44 - Extract iterative edges from k = 119 to 141 2026-08-03 09:49:44 - Build graph for k = 141 2026-08-03 09:49:45 - Assemble contigs from SdBG for k = 1412026-08-03 09:49:48 - Merging to output final contigs 2026-08-03 09:49:48 - 3785 contigs, total 1759217 bp, min 243 bp, max 10133 bp, avg 464 bp, N50 449 bp2026-08-03 09:49:48 - ALL DONE. Time elapsed: 128.612513 seconds
echo genes_catalogue_test && ln -s '/tmp/tmpy96jvlqm/files/8/9/5/dataset_89502fc5-67c8-40c6-82bf-e0370d0a8cf7.dat' 'pe1-genes_catalogue_test.fastqsanger.gz' && ln -s '/tmp/tmpy96jvlqm/files/5/4/a/dataset_54a460a2-e6ce-4524-9b4e-98bcf19898f8.dat' 'pe2-genes_catalogue_test.fastqsanger.gz' && metaquast --pe1 'pe1-genes_catalogue_test.fastqsanger.gz' --pe2 'pe2-genes_catalogue_test.fastqsanger.gz' --labels 'genes_catalogue_test' -o 'outputdir' --max-ref-num 0 --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage 'one' --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds '0,1000,5000,10000,25000,50000' --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 '/tmp/tmpy96jvlqm/files/5/7/3/dataset_57305f03-dbf4-438b-a90e-7598c7f79c13.dat' --threads ${GALAXY_SLOTS:-1} --no-krona && if [[ -f "outputdir/report.tsv" ]]; then mkdir -p "outputdir/combined_reference/" && cp "outputdir/report.tsv" "outputdir/combined_reference/report.tsv"; fi && if [[ -f "outputdir/report.html" ]]; then mkdir -p "outputdir/combined_reference/" && cp outputdir/*.html "outputdir/combined_reference/"; fi && mkdir -p '/tmp/tmpy96jvlqm/job_working_directory/000/6/outputs/dataset_bb4c81b5-323a-424a-a30f-5c37cdc77642_files' && cp outputdir/combined_reference/*.html '/tmp/tmpy96jvlqm/job_working_directory/000/6/outputs/dataset_bb4c81b5-323a-424a-a30f-5c37cdc77642_files' && if [[ -d "outputdir/icarus_viewers" ]]; then cp -R outputdir/icarus_viewers 'outputdir/combined_reference/'; fi && if [[ -d "outputdir/combined_reference/icarus_viewers" ]]; then cp -R outputdir/combined_reference/icarus_viewers '/tmp/tmpy96jvlqm/job_working_directory/000/6/outputs/dataset_bb4c81b5-323a-424a-a30f-5c37cdc77642_files'; fi && if [[ -d "outputdir/krona_charts/" ]]; then mkdir -p 'None' && cp outputdir/krona_charts/*.html 'None'; fi
Exit Code:
0
Standard Output:
genes_catalogue_test/usr/local/opt/quast-5.3.0/metaquast.py --pe1 pe1-genes_catalogue_test.fastqsanger.gz --pe2 pe2-genes_catalogue_test.fastqsanger.gz --labels genes_catalogue_test -o outputdir --max-ref-num 0 --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage one --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds 0,1000,5000,10000,25000,50000 --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 /tmp/tmpy96jvlqm/files/5/7/3/dataset_57305f03-dbf4-438b-a90e-7598c7f79c13.dat --threads 1 --no-kronaVersion: 5.3.0System information: OS: Linux-6.17.0-1020-azure-x86_64-with-glibc2.36 (linux_64) Python version: 3.12.3 CPUs number: 4Started: 2026-08-03 09:50:48Logging to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/metaquast.logWARNING: --ambiguity-usage was set to 'all' because not default --ambiguity-score was specifiedINFO generated new fontManagerINFO generated new fontManagerContigs: Pre-processing... /tmp/tmpy96jvlqm/files/5/7/3/dataset_57305f03-dbf4-438b-a90e-7598c7f79c13.dat ==> genes_catalogue_testNOTICE: Maximum number of references (--max-ref-number) is set to 0, search in SILVA 16S rRNA database is disabledNOTICE: No references are provided, starting regular QUAST with MetaGeneMark gene finder/usr/local/opt/quast-5.3.0/quast.py --pe1 pe1-genes_catalogue_test.fastqsanger.gz --pe2 pe2-genes_catalogue_test.fastqsanger.gz --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage one --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds 0,1000,5000,10000,25000,50000 --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 --threads 1 --no-krona /tmp/tmpy96jvlqm/files/5/7/3/dataset_57305f03-dbf4-438b-a90e-7598c7f79c13.dat -o /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir --labels genes_catalogue_testVersion: 5.3.0System information: OS: Linux-6.17.0-1020-azure-x86_64-with-glibc2.36 (linux_64) Python version: 3.12.3 CPUs number: 4Started: 2026-08-03 09:50:48Logging to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/quast.logNOTICE: Output directory already exists and looks like a QUAST output dir. Existing results can be reused (e.g. previously generated alignments)!WARNING: --ambiguity-usage was set to 'all' because not default --ambiguity-score was specifiedCWD: /tmp/tmpy96jvlqm/job_working_directory/000/6/workingMain parameters: MODE: meta, threads: 1, min contig length: 1500, min alignment length: 65, min alignment IDY: 95.0, \ ambiguity: all, min local misassembly length: 200, min extensive misassembly length: 1000Contigs: Pre-processing... /tmp/tmpy96jvlqm/files/5/7/3/dataset_57305f03-dbf4-438b-a90e-7598c7f79c13.dat ==> genes_catalogue_test2026-08-03 09:50:48Running Reads analyzer...NOTICE: Permission denied accessing /usr/local/lib/python3.12/site-packages/quast_libs/gridss. GRIDSS will be downloaded to home directory /tmp/tmpy96jvlqm/job_working_directory/000/6/home/.quastDownloading gridss (file: gridss-1.4.1.jar)... 0.0% of 38935087 bytes 1.0% of 38935087 bytes 2.0% of 38935087 bytes 3.0% of 38935087 bytes 4.0% of 38935087 bytes 5.0% of 38935087 bytes 6.0% of 38935087 bytes 7.0% of 38935087 bytes 8.0% of 38935087 bytes 9.0% of 38935087 bytes 10.0% of 38935087 bytes 11.0% of 38935087 bytes 12.0% of 38935087 bytes 13.0% of 38935087 bytes 14.0% of 38935087 bytes 15.0% of 38935087 bytes 16.0% of 38935087 bytes 17.0% of 38935087 bytes 18.0% of 38935087 bytes 19.0% of 38935087 bytes 20.0% of 38935087 bytes 21.0% of 38935087 bytes 22.0% of 38935087 bytes 23.0% of 38935087 bytes 24.0% of 38935087 bytes 25.0% of 38935087 bytes 26.0% of 38935087 bytes 27.0% of 38935087 bytes 28.0% of 38935087 bytes 29.0% of 38935087 bytes 30.0% of 38935087 bytes 31.0% of 38935087 bytes 32.0% of 38935087 bytes 33.0% of 38935087 bytes 34.0% of 38935087 bytes 35.0% of 38935087 bytes 36.0% of 38935087 bytes 37.0% of 38935087 bytes 38.0% of 38935087 bytes 39.0% of 38935087 bytes 40.0% of 38935087 bytes 41.0% of 38935087 bytes 42.0% of 38935087 bytes 43.0% of 38935087 bytes 44.0% of 38935087 bytes 45.0% of 38935087 bytes 46.0% of 38935087 bytes 47.0% of 38935087 bytes 48.0% of 38935087 bytes 49.0% of 38935087 bytes 50.0% of 38935087 bytes 51.0% of 38935087 bytes 52.0% of 38935087 bytes 53.0% of 38935087 bytes 54.0% of 38935087 bytes 55.0% of 38935087 bytes 56.0% of 38935087 bytes 57.0% of 38935087 bytes 58.0% of 38935087 bytes 59.0% of 38935087 bytes 60.0% of 38935087 bytes 61.0% of 38935087 bytes 62.0% of 38935087 bytes 63.0% of 38935087 bytes 64.0% of 38935087 bytes 65.0% of 38935087 bytes 66.0% of 38935087 bytes 67.0% of 38935087 bytes 68.0% of 38935087 bytes 69.0% of 38935087 bytes 70.0% of 38935087 bytes 71.0% of 38935087 bytes 72.0% of 38935087 bytes 73.0% of 38935087 bytes 74.0% of 38935087 bytes 75.0% of 38935087 bytes 76.0% of 38935087 bytes 77.0% of 38935087 bytes 78.0% of 38935087 bytes 79.0% of 38935087 bytes 80.0% of 38935087 bytes 81.0% of 38935087 bytes 82.0% of 38935087 bytes 83.0% of 38935087 bytes 84.0% of 38935087 bytes 85.0% of 38935087 bytes 86.0% of 38935087 bytes 87.0% of 38935087 bytes 88.0% of 38935087 bytes 88.0% of 38935087 bytes 89.0% of 38935087 bytes 90.0% of 38935087 bytes 91.0% of 38935087 bytes 92.0% of 38935087 bytes 93.0% of 38935087 bytes 94.0% of 38935087 bytes 95.0% of 38935087 bytes 96.0% of 38935087 bytes 97.0% of 38935087 bytes 98.0% of 38935087 bytes 99.0% of 38935087 bytesgridss successfully downloaded! Logging to files /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/reads_stats/reads_stats.log and /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/reads_stats/reads_stats.err... Pre-processing reads... Running BWA... Done. Sorting SAM-file... Analysis is finished. Creating total report... saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/reads_stats/reads_report.txt, reads_report.tsv, and reads_report.texDone.2026-08-03 09:51:27Running Basic statistics processor... Contig files: genes_catalogue_test Calculating N50 and L50... genes_catalogue_test, N50 = 2144, L50 = 11, auN = 3480.3, Total length = 73179, GC % = 41.99, # N's per 100 kbp = 0.00 Drawing Nx plot... saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/basic_stats/Nx_plot.pdf Drawing cumulative plot... saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/basic_stats/cumulative_plot.pdf Drawing GC content plot... saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/basic_stats/GC_content_plot.pdf Drawing genes_catalogue_test GC content plot... saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/basic_stats/genes_catalogue_test_GC_content_plot.pdfDone.NOTICE: Genes are not predicted by default. Use --gene-finding or --glimmer option to enable it.2026-08-03 09:51:28Creating large visual summaries...This may take a while: press Ctrl-C to skip this step.. 1 of 2: Creating PDF with all tables and plots... 2 of 2: Creating Icarus viewers...Done2026-08-03 09:51:28RESULTS: Text versions of total report are saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/report.txt, report.tsv, and report.tex Text versions of transposed total report are saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/transposed_report.txt, transposed_report.tsv, and transposed_report.tex HTML version (interactive tables and plots) is saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/report.html PDF version (tables and plots) is saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/report.pdf Icarus (contig browser) is saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/icarus.html Log is saved to /tmp/tmpy96jvlqm/job_working_directory/000/6/working/outputdir/quast.logFinished: 2026-08-03 09:51:28Elapsed time: 0:00:39.919329NOTICEs: 3; WARNINGs: 1; non-fatal ERRORs: 0Thank you for using QUAST!
Using nucl database resfinder: 3206 sequences - 2026-Apr-3Processing: genes_catalogue_testFound 1 genes in genes_catalogue_testTip: you can use the --summary option to combine reports in a presence/absence matrix.Done.
2026-08-03 09:54:12 WARNING: Using non-default ResFinder/PointFinder. This may lead to differences in the detected AMR genes depending on how the database files are structured.2026-08-03 09:54:12 INFO: No --pointfinder-organism specified. Will not search the PointFinder databases2026-08-03 09:54:12 INFO: No --plasmidfinder-database-type specified. Will search the entire PlasmidFinder database2026-08-03 09:54:12 INFO: --output-dir not set. Files will be output to the respective --output-[type] setting2026-08-03 09:54:12 INFO: Will exclude ResFinder/PointFinder genes listed in [/usr/local/lib/python3.14/site-packages/staramr/databases/exclude/data/genes_to_exclude.tsv]. Use --no-exclude-genes to disable2026-08-03 09:54:12 INFO: Will report complex mutations listed in [/usr/local/lib/python3.14/site-packages/staramr/databases/resistance/pointfinder/complex/data/complex_mutations.tsv]2026-08-03 09:54:12 INFO: Making BLAST databases for input files2026-08-03 09:54:12 INFO: Scheduling blasts and MLST for genes_catalogue_test.fasta2026-08-03 09:54:17 WARNING: No drug found for drug_class=all, gene=cfxA_1, accession=U382432026-08-03 09:54:18 INFO: Finished. Took 0.10 minutes.2026-08-03 09:54:19 INFO: Predicting AMR resistance phenotypes is enabled. The predictions are for microbiological resistance and *not* clinical resistance. These results are continually being improved and we welcome any feedback.2026-08-03 09:54:19 INFO: Writing resfinder to [/tmp/tmpy96jvlqm/job_working_directory/000/16/outputs/dataset_df3f6a6e-5f4e-4649-9c39-33004ce2a432.dat]2026-08-03 09:54:19 INFO: --output-dir or --output-pointfinder unset. No pointfinder file will be written2026-08-03 09:54:19 INFO: Writing plasmidfinder to [/tmp/tmpy96jvlqm/job_working_directory/000/16/outputs/dataset_683de87f-04e2-449b-bc5c-f460fd53b285.dat]2026-08-03 09:54:19 INFO: Writing summary to [/tmp/tmpy96jvlqm/job_working_directory/000/16/outputs/dataset_c4c5fe2c-4ae2-43ea-be22-0b29f2c7c1a8.dat]2026-08-03 09:54:19 INFO: Writing MLST summary to [/tmp/tmpy96jvlqm/job_working_directory/000/16/outputs/dataset_8e2315eb-d750-44f0-9924-7e4fe22235a8.dat]2026-08-03 09:54:19 INFO: Writing detailed summary to [/tmp/tmpy96jvlqm/job_working_directory/000/16/outputs/dataset_b863f4a0-9303-4f89-9157-ec08b67e72b7.dat]2026-08-03 09:54:19 INFO: Writing settings to [XXXX]2026-08-03 09:54:19 INFO: Writing Excel to [results.xlsx]2026-08-03 09:54:19 INFO: BLAST hits are stored in [staramr_hits]
gxydevbot
changed the title
Updating workflows/microbiome/metagenomic-genes-catalogue from 1.3 to 1.4
Updating workflows/microbiome/metagenomic-genes-catalogue from 1.3 to 1.4
Aug 10, 2026
2026-08-10 09:50:17 - MEGAHIT v1.2.92026-08-10 09:50:17 - Using megahit_core with POPCNT and BMI2 support2026-08-10 09:50:17 - Convert reads to binary library2026-08-10 09:50:18 - b'INFO sequence/io/sequence_lib.cpp : 75 - Lib 0 (/tmp/tmpmaxbzsnl/files/b/f/7/dataset_bf74af05-127a-4746-ae2c-3c539b422924.dat,/tmp/tmpmaxbzsnl/files/6/4/3/dataset_64302efd-72c8-4833-b8b4-dddbbd6d0794.dat): pe, 500000 reads, 101 max length'2026-08-10 09:50:18 - b'INFO utils/utils.h : 152 - Real: 0.6668\tuser: 0.6386\tsys: 0.0280\tmaxrss: 28780'2026-08-10 09:50:18 - Start assembly. Number of CPU threads 1 2026-08-10 09:50:18 - k list: 21,29,39,59,79,99,119,141 2026-08-10 09:50:18 - Memory used: 150897844222026-08-10 09:50:18 - Extract solid (k+1)-mers for k = 21 2026-08-10 09:50:28 - Build graph for k = 21 2026-08-10 09:50:37 - Assemble contigs from SdBG for k = 212026-08-10 09:51:08 - Local assembly for k = 212026-08-10 09:51:11 - Extract iterative edges from k = 21 to 29 2026-08-10 09:51:13 - Build graph for k = 29 2026-08-10 09:51:16 - Assemble contigs from SdBG for k = 292026-08-10 09:51:36 - Local assembly for k = 292026-08-10 09:51:40 - Extract iterative edges from k = 29 to 39 2026-08-10 09:51:41 - Build graph for k = 39 2026-08-10 09:51:43 - Assemble contigs from SdBG for k = 392026-08-10 09:51:57 - Local assembly for k = 392026-08-10 09:52:00 - Extract iterative edges from k = 39 to 59 2026-08-10 09:52:02 - Build graph for k = 59 2026-08-10 09:52:03 - Assemble contigs from SdBG for k = 592026-08-10 09:52:11 - Local assembly for k = 592026-08-10 09:52:15 - Extract iterative edges from k = 59 to 79 2026-08-10 09:52:15 - Build graph for k = 79 2026-08-10 09:52:16 - Assemble contigs from SdBG for k = 792026-08-10 09:52:22 - Local assembly for k = 792026-08-10 09:52:25 - Extract iterative edges from k = 79 to 99 2026-08-10 09:52:26 - Build graph for k = 99 2026-08-10 09:52:27 - Assemble contigs from SdBG for k = 992026-08-10 09:52:31 - Local assembly for k = 992026-08-10 09:52:34 - Extract iterative edges from k = 99 to 119 2026-08-10 09:52:34 - Build graph for k = 119 2026-08-10 09:52:35 - Assemble contigs from SdBG for k = 1192026-08-10 09:52:38 - Local assembly for k = 1192026-08-10 09:52:41 - Extract iterative edges from k = 119 to 141 2026-08-10 09:52:41 - Build graph for k = 141 2026-08-10 09:52:41 - Assemble contigs from SdBG for k = 1412026-08-10 09:52:44 - Merging to output final contigs 2026-08-10 09:52:44 - 3785 contigs, total 1759217 bp, min 243 bp, max 10133 bp, avg 464 bp, N50 449 bp2026-08-10 09:52:44 - ALL DONE. Time elapsed: 146.823895 seconds
echo genes_catalogue_test && ln -s '/tmp/tmpmaxbzsnl/files/b/f/7/dataset_bf74af05-127a-4746-ae2c-3c539b422924.dat' 'pe1-genes_catalogue_test.fastqsanger.gz' && ln -s '/tmp/tmpmaxbzsnl/files/6/4/3/dataset_64302efd-72c8-4833-b8b4-dddbbd6d0794.dat' 'pe2-genes_catalogue_test.fastqsanger.gz' && metaquast --pe1 'pe1-genes_catalogue_test.fastqsanger.gz' --pe2 'pe2-genes_catalogue_test.fastqsanger.gz' --labels 'genes_catalogue_test' -o 'outputdir' --max-ref-num 0 --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage 'one' --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds '0,1000,5000,10000,25000,50000' --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 '/tmp/tmpmaxbzsnl/files/f/6/a/dataset_f6adf6f8-1749-44ba-bf42-85bdd2db4bbb.dat' --threads ${GALAXY_SLOTS:-1} --no-krona && if [[ -f "outputdir/report.tsv" ]]; then mkdir -p "outputdir/combined_reference/" && cp "outputdir/report.tsv" "outputdir/combined_reference/report.tsv"; fi && if [[ -f "outputdir/report.html" ]]; then mkdir -p "outputdir/combined_reference/" && cp outputdir/*.html "outputdir/combined_reference/"; fi && mkdir -p '/tmp/tmpmaxbzsnl/job_working_directory/000/6/outputs/dataset_5a6bea47-9ebc-46fd-9e1a-2d50d85890a2_files' && cp outputdir/combined_reference/*.html '/tmp/tmpmaxbzsnl/job_working_directory/000/6/outputs/dataset_5a6bea47-9ebc-46fd-9e1a-2d50d85890a2_files' && if [[ -d "outputdir/icarus_viewers" ]]; then cp -R outputdir/icarus_viewers 'outputdir/combined_reference/'; fi && if [[ -d "outputdir/combined_reference/icarus_viewers" ]]; then cp -R outputdir/combined_reference/icarus_viewers '/tmp/tmpmaxbzsnl/job_working_directory/000/6/outputs/dataset_5a6bea47-9ebc-46fd-9e1a-2d50d85890a2_files'; fi && if [[ -d "outputdir/krona_charts/" ]]; then mkdir -p 'None' && cp outputdir/krona_charts/*.html 'None'; fi
Exit Code:
0
Standard Output:
genes_catalogue_test/usr/local/opt/quast-5.3.0/metaquast.py --pe1 pe1-genes_catalogue_test.fastqsanger.gz --pe2 pe2-genes_catalogue_test.fastqsanger.gz --labels genes_catalogue_test -o outputdir --max-ref-num 0 --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage one --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds 0,1000,5000,10000,25000,50000 --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 /tmp/tmpmaxbzsnl/files/f/6/a/dataset_f6adf6f8-1749-44ba-bf42-85bdd2db4bbb.dat --threads 1 --no-kronaVersion: 5.3.0System information: OS: Linux-6.17.0-1020-azure-x86_64-with-glibc2.36 (linux_64) Python version: 3.12.3 CPUs number: 4Started: 2026-08-10 09:53:43Logging to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/metaquast.logWARNING: --ambiguity-usage was set to 'all' because not default --ambiguity-score was specifiedINFO generated new fontManagerINFO generated new fontManagerContigs: Pre-processing... /tmp/tmpmaxbzsnl/files/f/6/a/dataset_f6adf6f8-1749-44ba-bf42-85bdd2db4bbb.dat ==> genes_catalogue_testNOTICE: Maximum number of references (--max-ref-number) is set to 0, search in SILVA 16S rRNA database is disabledNOTICE: No references are provided, starting regular QUAST with MetaGeneMark gene finder/usr/local/opt/quast-5.3.0/quast.py --pe1 pe1-genes_catalogue_test.fastqsanger.gz --pe2 pe2-genes_catalogue_test.fastqsanger.gz --min-identity 95.0 --min-contig 1500 --min-alignment 65 --ambiguity-usage one --ambiguity-score 0.99 --local-mis-size 200 --contig-thresholds 0,1000,5000,10000,25000,50000 --extensive-mis-size 1000 --scaffold-gap-max-size 10000 --unaligned-part-size 500 --x-for-Nx 90 --threads 1 --no-krona /tmp/tmpmaxbzsnl/files/f/6/a/dataset_f6adf6f8-1749-44ba-bf42-85bdd2db4bbb.dat -o /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir --labels genes_catalogue_testVersion: 5.3.0System information: OS: Linux-6.17.0-1020-azure-x86_64-with-glibc2.36 (linux_64) Python version: 3.12.3 CPUs number: 4Started: 2026-08-10 09:53:44Logging to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/quast.logNOTICE: Output directory already exists and looks like a QUAST output dir. Existing results can be reused (e.g. previously generated alignments)!WARNING: --ambiguity-usage was set to 'all' because not default --ambiguity-score was specifiedCWD: /tmp/tmpmaxbzsnl/job_working_directory/000/6/workingMain parameters: MODE: meta, threads: 1, min contig length: 1500, min alignment length: 65, min alignment IDY: 95.0, \ ambiguity: all, min local misassembly length: 200, min extensive misassembly length: 1000Contigs: Pre-processing... /tmp/tmpmaxbzsnl/files/f/6/a/dataset_f6adf6f8-1749-44ba-bf42-85bdd2db4bbb.dat ==> genes_catalogue_test2026-08-10 09:53:44Running Reads analyzer...NOTICE: Permission denied accessing /usr/local/lib/python3.12/site-packages/quast_libs/gridss. GRIDSS will be downloaded to home directory /tmp/tmpmaxbzsnl/job_working_directory/000/6/home/.quastDownloading gridss (file: gridss-1.4.1.jar)... 0.0% of 38935087 bytes 1.0% of 38935087 bytes 2.0% of 38935087 bytes 3.0% of 38935087 bytes 4.0% of 38935087 bytes 5.0% of 38935087 bytes 6.0% of 38935087 bytes 7.0% of 38935087 bytes 8.0% of 38935087 bytes 9.0% of 38935087 bytes 10.0% of 38935087 bytes 11.0% of 38935087 bytes 12.0% of 38935087 bytes 13.0% of 38935087 bytes 14.0% of 38935087 bytes 15.0% of 38935087 bytes 16.0% of 38935087 bytes 17.0% of 38935087 bytes 18.0% of 38935087 bytes 19.0% of 38935087 bytes 20.0% of 38935087 bytes 21.0% of 38935087 bytes 22.0% of 38935087 bytes 23.0% of 38935087 bytes 24.0% of 38935087 bytes 25.0% of 38935087 bytes 26.0% of 38935087 bytes 27.0% of 38935087 bytes 28.0% of 38935087 bytes 29.0% of 38935087 bytes 30.0% of 38935087 bytes 31.0% of 38935087 bytes 32.0% of 38935087 bytes 33.0% of 38935087 bytes 34.0% of 38935087 bytes 35.0% of 38935087 bytes 36.0% of 38935087 bytes 37.0% of 38935087 bytes 38.0% of 38935087 bytes 39.0% of 38935087 bytes 40.0% of 38935087 bytes 41.0% of 38935087 bytes 42.0% of 38935087 bytes 43.0% of 38935087 bytes 44.0% of 38935087 bytes 45.0% of 38935087 bytes 46.0% of 38935087 bytes 47.0% of 38935087 bytes 48.0% of 38935087 bytes 49.0% of 38935087 bytes 50.0% of 38935087 bytes 51.0% of 38935087 bytes 52.0% of 38935087 bytes 53.0% of 38935087 bytes 54.0% of 38935087 bytes 55.0% of 38935087 bytes 56.0% of 38935087 bytes 57.0% of 38935087 bytes 58.0% of 38935087 bytes 59.0% of 38935087 bytes 60.0% of 38935087 bytes 61.0% of 38935087 bytes 62.0% of 38935087 bytes 63.0% of 38935087 bytes 64.0% of 38935087 bytes 65.0% of 38935087 bytes 66.0% of 38935087 bytes 67.0% of 38935087 bytes 68.0% of 38935087 bytes 69.0% of 38935087 bytes 70.0% of 38935087 bytes 71.0% of 38935087 bytes 72.0% of 38935087 bytes 73.0% of 38935087 bytes 74.0% of 38935087 bytes 75.0% of 38935087 bytes 76.0% of 38935087 bytes 77.0% of 38935087 bytes 78.0% of 38935087 bytes 79.0% of 38935087 bytes 80.0% of 38935087 bytes 81.0% of 38935087 bytes 82.0% of 38935087 bytes 83.0% of 38935087 bytes 84.0% of 38935087 bytes 85.0% of 38935087 bytes 86.0% of 38935087 bytes 87.0% of 38935087 bytes 88.0% of 38935087 bytes 88.0% of 38935087 bytes 89.0% of 38935087 bytes 90.0% of 38935087 bytes 91.0% of 38935087 bytes 92.0% of 38935087 bytes 93.0% of 38935087 bytes 94.0% of 38935087 bytes 95.0% of 38935087 bytes 96.0% of 38935087 bytes 97.0% of 38935087 bytes 98.0% of 38935087 bytes 99.0% of 38935087 bytesgridss successfully downloaded! Logging to files /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/reads_stats/reads_stats.log and /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/reads_stats/reads_stats.err... Pre-processing reads... Running BWA... Done. Sorting SAM-file... Analysis is finished. Creating total report... saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/reads_stats/reads_report.txt, reads_report.tsv, and reads_report.texDone.2026-08-10 09:54:24Running Basic statistics processor... Contig files: genes_catalogue_test Calculating N50 and L50... genes_catalogue_test, N50 = 2144, L50 = 11, auN = 3480.3, Total length = 73179, GC % = 41.99, # N's per 100 kbp = 0.00 Drawing Nx plot... saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/basic_stats/Nx_plot.pdf Drawing cumulative plot... saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/basic_stats/cumulative_plot.pdf Drawing GC content plot... saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/basic_stats/GC_content_plot.pdf Drawing genes_catalogue_test GC content plot... saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/basic_stats/genes_catalogue_test_GC_content_plot.pdfDone.NOTICE: Genes are not predicted by default. Use --gene-finding or --glimmer option to enable it.2026-08-10 09:54:25Creating large visual summaries...This may take a while: press Ctrl-C to skip this step.. 1 of 2: Creating PDF with all tables and plots... 2 of 2: Creating Icarus viewers...Done2026-08-10 09:54:25RESULTS: Text versions of total report are saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/report.txt, report.tsv, and report.tex Text versions of transposed total report are saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/transposed_report.txt, transposed_report.tsv, and transposed_report.tex HTML version (interactive tables and plots) is saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/report.html PDF version (tables and plots) is saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/report.pdf Icarus (contig browser) is saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/icarus.html Log is saved to /tmp/tmpmaxbzsnl/job_working_directory/000/6/working/outputdir/quast.logFinished: 2026-08-10 09:54:25Elapsed time: 0:00:41.608378NOTICEs: 3; WARNINGs: 1; non-fatal ERRORs: 0Thank you for using QUAST!
Using nucl database resfinder: 3206 sequences - 2026-Apr-3Processing: genes_catalogue_testFound 1 genes in genes_catalogue_testTip: found a bug in abricate? Post it at https://github.com/tseemann/abricate/issues.Done.
2026-08-10 09:57:25 WARNING: Using non-default ResFinder/PointFinder. This may lead to differences in the detected AMR genes depending on how the database files are structured.2026-08-10 09:57:25 INFO: No --pointfinder-organism specified. Will not search the PointFinder databases2026-08-10 09:57:25 INFO: No --plasmidfinder-database-type specified. Will search the entire PlasmidFinder database2026-08-10 09:57:25 INFO: --output-dir not set. Files will be output to the respective --output-[type] setting2026-08-10 09:57:25 INFO: Will exclude ResFinder/PointFinder genes listed in [/usr/local/lib/python3.14/site-packages/staramr/databases/exclude/data/genes_to_exclude.tsv]. Use --no-exclude-genes to disable2026-08-10 09:57:25 INFO: Will report complex mutations listed in [/usr/local/lib/python3.14/site-packages/staramr/databases/resistance/pointfinder/complex/data/complex_mutations.tsv]2026-08-10 09:57:25 INFO: Making BLAST databases for input files2026-08-10 09:57:25 INFO: Scheduling blasts and MLST for genes_catalogue_test.fasta2026-08-10 09:57:36 WARNING: No drug found for drug_class=all, gene=cfxA_1, accession=U382432026-08-10 09:57:39 INFO: Finished. Took 0.24 minutes.2026-08-10 09:57:44 INFO: Predicting AMR resistance phenotypes is enabled. The predictions are for microbiological resistance and *not* clinical resistance. These results are continually being improved and we welcome any feedback.2026-08-10 09:57:44 INFO: Writing resfinder to [/tmp/tmpmaxbzsnl/job_working_directory/000/16/outputs/dataset_19382bab-0573-4171-8b2b-314e35684cf1.dat]2026-08-10 09:57:44 INFO: --output-dir or --output-pointfinder unset. No pointfinder file will be written2026-08-10 09:57:44 INFO: Writing plasmidfinder to [/tmp/tmpmaxbzsnl/job_working_directory/000/16/outputs/dataset_2c5324cf-1bff-433d-9949-996afa4d90b8.dat]2026-08-10 09:57:44 INFO: Writing summary to [/tmp/tmpmaxbzsnl/job_working_directory/000/16/outputs/dataset_c2197c24-9e1a-4c1f-ac5d-37465acb3fc0.dat]2026-08-10 09:57:44 INFO: Writing MLST summary to [/tmp/tmpmaxbzsnl/job_working_directory/000/16/outputs/dataset_1c8a50dd-330e-48c4-a8eb-99b9bfddc19b.dat]2026-08-10 09:57:44 INFO: Writing detailed summary to [/tmp/tmpmaxbzsnl/job_working_directory/000/16/outputs/dataset_a8e0850f-2f2e-44ff-b174-775380c3c3f5.dat]2026-08-10 09:57:44 INFO: Writing settings to [XXXX]2026-08-10 09:57:44 INFO: Writing Excel to [results.xlsx]2026-08-10 09:57:44 INFO: BLAST hits are stored in [staramr_hits]
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Hello! This is an automated update of the following workflow: workflows/microbiome/metagenomic-genes-catalogue. I created this PR because I think one or more of the component tools are out of date, i.e. there is a newer version available on the ToolShed.
By comparing with the latest versions available on the ToolShed, it seems the following tools are outdated:
toolshed.g2.bx.psu.edu/repos/iuc/amrfinderplus/amrfinderplus/3.12.8+galaxy0should be updated totoolshed.g2.bx.psu.edu/repos/iuc/amrfinderplus/amrfinderplus/4.2.7+galaxy0toolshed.g2.bx.psu.edu/repos/iuc/coverm_contig/coverm_contig/0.8.0+galaxy0should be updated totoolshed.g2.bx.psu.edu/repos/iuc/coverm_contig/coverm_contig/0.8.0+galaxy1toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.35+galaxy1should be updated totoolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.35+galaxy2The workflow release number has been updated from 1.3 to 1.4.
If you want to skip this change, close this PR without deleting the branch. It will be reopened if another change is detected.
Any commit from another author than 'planemo-autoupdate' will prevent more auto-updates.
To ignore manual changes and allow autoupdates, delete the branch.