AllenCell · meganrm · Jul 31, 2026 · Jul 31, 2026
diff --git a/src/pages/cell-line/AICS-90-391/index.md b/src/pages/cell-line/AICS-90-391/index.md
@@ -13,13 +13,17 @@ genetic_modifications:
 order_link: https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AICS-0090-391&PgId=166
 certificate_of_analysis: https://www.coriell.org/0/PDF/Allen/ipsc/AICS-0090-391_CofA.pdf
 donor_plasmid: https://www.addgene.org/127968/
-hpscreg_certificate_link: https://hpscreg.eu/cell-line/UCSFi001-A-45
 images_and_videos:
   images:
     - image: single_plane_image_cl391.jpg
-      caption: "Single, mid-level plane of cells in a live hiPS cell colony expressing TagBFP-tagged dCas9-KRAB. TagBFP-tagged dCas9-KRAB (left) and overlay onto transmitted light image (right) are shown. Cells were imaged in 3D on a spinning-disk confocal microscope. Scale bar, 50 μm."
+      caption: Single, mid-level plane of cells in a live hiPS cell colony expressing
+        TagBFP-tagged dCas9-KRAB. TagBFP-tagged dCas9-KRAB (left) and overlay
+        onto transmitted light image (right) are shown. Cells were imaged in 3D
+        on a spinning-disk confocal microscope. Scale bar, 50 μm.
     - image: Main_cell_line_morphology.jpg
-      caption: "Viability and colony formation one day and three days post-thaw. Cells were treated with ROCK inhibitor for 24 hrs post-thaw."
+      caption: Viability and colony formation one day and three days post-thaw. Cells
+        were treated with ROCK inhibitor for 24 hrs post-thaw.
+    - image: WesternRelease_AICS-0090_dCas9-KRAB.jpg
   videos:
     - video: https://player.vimeo.com/video/1079587233
       caption: Z-stack of live hiPS cell colony expressing TagBFP-tagged dCas9-KRAB.
@@ -34,74 +38,96 @@ editing_design:
   linker: N/A
   cas9: Wildtype spCas9
   diagrams:
-    - title: "mEGFP Insert"
+    - title: mEGFP Insert
       images:
         - image: EditingDesign_gene_figure.png
-          caption: "Top: CLYBL locus; Bottom: Zoom in on dCas9-TagBFP-KRAB insertion site at CLYBL safe harbor site between exons 2 and 3"
-category_labels: 
- - Tools
+          caption: "Top: CLYBL locus; Bottom: Zoom in on dCas9-TagBFP-KRAB insertion site
+            at CLYBL safe harbor site between exons 2 and 3"
 genomic_characterization:
   diagrams:
-    - title: "Schematic of Junctions"
+    - title: Schematic of Junctions
       images:
         - image: /img/shared/GenomicCharacterization_junction_schematic_generic_insert.png
-    - title: "Karyotype Analysis"
+    - title: Karyotype Analysis
       images:
         - image: AICS-90_cl391_CLYBL-dCas9-KRAB_karyotype.JPG
-          caption: "After cells banks were created, one vial was thawed and 30 G-banded metaphase cells were karyotyped."
+          caption: After cells banks were created, one vial was thawed and 30 G-banded
+            metaphase cells were karyotyped.
   amplified_junctions:
-    - edited_gene: "dCas9-KRAB-BFP"
-      junction: "5'"
+    - edited_gene: dCas9-KRAB-BFP
+      junction: 5'
       expected_size: "1942"
-      confirmed_sequence: "Yes"
-    - edited_gene: "dCas9-KRAB-BFP"
-      junction: "3'"
+      confirmed_sequence: Yes
+    - edited_gene: dCas9-KRAB-BFP
+      junction: 3'
       expected_size: "3232"
-      confirmed_sequence: "Yes"
-    - edited_gene: "dCas9-KRAB-BFP"
-      junction: "WT internal"
+      confirmed_sequence: Yes
+    - edited_gene: dCas9-KRAB-BFP
+      junction: WT internal
       expected_size: "2232"
-      confirmed_sequence: "Yes"
-    - edited_gene: "dCas9-KRAB-BFP"
-      junction: "Full junctional allele"
-      expected_size: "Not performed"
+      confirmed_sequence: Yes
+    - edited_gene: dCas9-KRAB-BFP
+      junction: Full junctional allele
+      expected_size: Not performed
       confirmed_sequence: ""
-  junction_table_caption: "PCR amplified 5', 3', WT, and full allele junctions. 5', 3', and WT junctions were Sanger sequenced to check for precise mEGFP insertion. Primers were designed to exclude amplification from the donor plasmid."
+  junction_table_caption: PCR amplified 5', 3', WT, and full allele junctions. 5',
+    3', and WT junctions were Sanger sequenced to check for precise mEGFP
+    insertion. Primers were designed to exclude amplification from the donor
+    plasmid.
   ddpcr:
     - tag: CLYBL-dCas9-TagBFP-KRAB
       clone: 391
       fp_ratio: 0.54
-      plasmid: 0.0
-  ddpcr_caption: "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. Plasmid: KAN/RPP30 ratio from ddPCR assay; values <0.1 indicate clone with no detectable plasmid integration. RPP30 is known 2n reference gene."
+      plasmid: 0
+  ddpcr_caption: "FP:RPP30 ratio from ddPCR assay; values = 0.5 +/- 0.1 indicate
+    heterozygous clone, values = 1 +/- 0.1 indicate homozygous clone. Plasmid:
+    KAN/RPP30 ratio from ddPCR assay; values <0.1 indicate clone with no
+    detectable plasmid integration. RPP30 is known 2n reference gene."
+category_labels:
+  - Tools
+hpscreg_certificate_link: https://hpscreg.eu/cell-line/UCSFi001-A-45
 stem_cell_characteristics:
   pluripotency_analysis:
-    - marker: "NANOG"
+    - marker: NANOG
       positive_cells: 99.9
-    - marker: "SOX2"
+    - marker: SOX2
       positive_cells: null
-    - marker: "OCT4"
+    - marker: OCT4
       positive_cells: 100
-    - marker: "SSEA-1"
+    - marker: SSEA-1
       positive_cells: null
-    - marker: "SSEA-4"
+    - marker: SSEA-4
       positive_cells: 100
-    - marker: "TRA-160"
+    - marker: TRA-160
       positive_cells: 100
-  pluripotency_caption: "iPSCs were stained with directly conjugated antibodies from BD Biosciences, acquired using a FACSAria III Fusion (BD Biosciences), and analyzed using FlowJo software (Treestar, Inc.). Doublets were excluded, then marker-specific gates were set according to corresponding fluorescence-minus-one (FMO) controls."
+  pluripotency_caption: iPSCs were stained with directly conjugated antibodies
+    from BD Biosciences, acquired using a FACSAria III Fusion (BD Biosciences),
+    and analyzed using FlowJo software (Treestar, Inc.). Doublets were excluded,
+    then marker-specific gates were set according to corresponding
+    fluorescence-minus-one (FMO) controls.
   trilineage_differentiation:
-    - germ_layer: "Ectoderm"
-      marker: "PAX6"
+    - germ_layer: Ectoderm
+      marker: PAX6
       percent_positive_cells: Pass
-    - germ_layer: "Endoderm"
-      marker: "SOX17"
+    - germ_layer: Endoderm
+      marker: SOX17
       percent_positive_cells: Pass
-    - germ_layer: "Mesoderm"
-      marker: "Brachyury"
+    - germ_layer: Mesoderm
+      marker: Brachyury
       percent_positive_cells: Pass
-  trilineage_caption: "iPSCs were subjected to a 5-7 day, non-terminal, directed differentiation using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL Technologies, Inc.). Total RNA was isolated from each lineage specific differentiation and assayed via ddPCR for the expression of lineage specific transcripts; Pax6(Ectoderm), Sox17(Endoderm) and Brachyury(Mesoderm)."
+  trilineage_caption: iPSCs were subjected to a 5-7 day, non-terminal, directed
+    differentiation using the STEMdiff™ Trilineage Differentiation Kit (STEMCELL
+    Technologies, Inc.). Total RNA was isolated from each lineage specific
+    differentiation and assayed via ddPCR for the expression of lineage specific
+    transcripts; Pax6(Ectoderm), Sox17(Endoderm) and Brachyury(Mesoderm).
   cardiomyocyte_differentiation:
-    troponin_percent_positive: "72.6 (3)"
-    day_of_beating_percent: "100 (4)"
-    day_of_beating_range: "d10-d11"
-  cardiomyocyte_differentiation_caption: "iPSCs were differentiated to cardiomyocytes and observed for initiation of beating starting at day 6. At ~day 12, cells were fixed and stained with anti-cardiac Troponin T (BD Biosciences) and gating was based on an isotype control. Ranges observed across multiple experiments are shown for Troponin T and Day of beating initiation; number of experiments is shown in (). "
----
+    troponin_percent_positive: 72.6 (3)
+    day_of_beating_percent: 100 (4)
+    day_of_beating_range: d10-d11
+  cardiomyocyte_differentiation_caption: "iPSCs were differentiated to
+    cardiomyocytes and observed for initiation of beating starting at day 6. At
+    ~day 12, cells were fixed and stained with anti-cardiac Troponin T (BD
+    Biosciences) and gating was based on an isotype control. Ranges observed
+    across multiple experiments are shown for Troponin T and Day of beating
+    initiation; number of experiments is shown in (). "
+---