diff --git a/nox/virtualenv.py b/nox/virtualenv.py
index 80776a47..6909e4cf 100644
--- a/nox/virtualenv.py
+++ b/nox/virtualenv.py
@@ -733,11 +733,7 @@ def _clean_location(self) -> bool:
     def _check_reused_environment_links(self) -> bool:
         """Check that interpreter links in the reused environment are not broken."""
         bin_dir = self.bin
-        names = (
-            ["python.exe"]
-            if _PLATFORM.startswith("win") and not _IS_MINGW
-            else ["python", "python3"]
-        )
+        names = ["python.exe"] if self._windows_layout else ["python", "python3"]
 
         for name in names:
             path = os.path.join(bin_dir, name)
@@ -895,10 +891,21 @@ def _install_python(self, cleaned_interpreter: str) -> str | None:
             return None
         return pbs_install_python(cleaned_interpreter)
 
+    @property
+    def _windows_layout(self) -> bool:
+        """Whether the venv uses a Windows-style layout (``Scripts``/``python.exe``).
+
+        uv always builds a Windows-style venv, even under MSYS2/MinGW, where the
+        native interpreter and virtualenv/venv use a POSIX layout (``bin``/``python``).
+        """
+        if not _PLATFORM.startswith("win"):
+            return False
+        return not _IS_MINGW or self.venv_backend == "uv"
+
     @property
     def bin_paths(self) -> list[str]:
         """Returns the location of the virtualenv's bin folder."""
-        if _PLATFORM.startswith("win") and not _IS_MINGW:
+        if self._windows_layout:
             return [os.path.join(self.location, "Scripts")]
         return [os.path.join(self.location, "bin")]
 
@@ -930,13 +937,14 @@ def create(self) -> bool:
                     cmd.extend(["-p", self._resolved_interpreter])
             case "uv":
                 _, uv, uv_ver = _uv_state()
-                cmd = [
-                    uv,
-                    "venv",
-                    "-p",
-                    self._resolved_interpreter if self.interpreter else sys.executable,
-                    self.location,
-                ]
+                cmd = [uv, "venv"]
+                if self.interpreter:
+                    cmd += ["-p", self._resolved_interpreter]
+                elif not _IS_MINGW:
+                    # uv can't inspect the MSYS2/MinGW interpreter, so let it
+                    # pick the default rather than passing sys.executable.
+                    cmd += ["-p", sys.executable]
+                cmd += [self.location]
                 if version.Version("0.8") <= uv_ver:
                     cmd += ["--clear"]
             case _:
diff --git a/tests/test_virtualenv.py b/tests/test_virtualenv.py
index b195abc9..31226d21 100644
--- a/tests/test_virtualenv.py
+++ b/tests/test_virtualenv.py
@@ -465,6 +465,41 @@ def test_create_args_old_uv(
     assert run_mock.call_args.args[0][-1] != "--clear"
 
 
+def test_create_uv_with_interpreter(
+    make_one: Callable[..., tuple[VirtualEnv, Path]],
+    monkeypatch: pytest.MonkeyPatch,
+) -> None:
+    run_mock = mock.Mock()
+    monkeypatch.setattr(nox.command, "run", run_mock)
+    monkeypatch.setattr(nox.virtualenv, "UV", "uv")
+    monkeypatch.setattr(nox.virtualenv, "HAS_UV", True)
+    monkeypatch.setattr(nox.virtualenv, "UV_VERSION", version.Version("0.10.0"))
+    venv, _ = make_one(interpreter="3.12", venv_backend="uv")
+    venv._resolved = "/path/to/python3.12"
+    venv.create()
+    run_mock.assert_called_once()
+    cmd = run_mock.call_args.args[0]
+    assert cmd[cmd.index("-p") + 1] == "/path/to/python3.12"
+
+
+@mock.patch("nox.virtualenv._PLATFORM", new="win32")
+@mock.patch("nox.virtualenv._IS_MINGW", new=True)
+def test_create_uv_mingw_no_interpreter(
+    make_one: Callable[..., tuple[VirtualEnv, Path]],
+    monkeypatch: pytest.MonkeyPatch,
+) -> None:
+    # uv can't inspect the MSYS2/MinGW interpreter, so nox must not pass ``-p``.
+    run_mock = mock.Mock()
+    monkeypatch.setattr(nox.command, "run", run_mock)
+    monkeypatch.setattr(nox.virtualenv, "UV", "uv")
+    monkeypatch.setattr(nox.virtualenv, "HAS_UV", True)
+    monkeypatch.setattr(nox.virtualenv, "UV_VERSION", version.Version("0.10.0"))
+    venv, _ = make_one(venv_backend="uv")
+    venv.create()
+    run_mock.assert_called_once()
+    assert "-p" not in run_mock.call_args.args[0]
+
+
 @has_uv
 def test_uv_creation(
     make_one: Callable[..., tuple[VirtualEnv, Path]],
@@ -617,6 +652,19 @@ def test_bin_windows_mingw(
     assert str(dir_.joinpath("bin")) == venv.bin
 
 
+@mock.patch("nox.virtualenv._PLATFORM", new="win32")
+@mock.patch("nox.virtualenv._IS_MINGW", new=True)
+def test_bin_windows_mingw_uv(
+    make_one: Callable[..., tuple[VirtualEnv | ProcessEnv, Path]],
+) -> None:
+    # uv builds a Windows-style venv even under MinGW, unlike the native tools.
+    venv, dir_ = make_one(venv_backend="uv")
+    assert venv.bin_paths
+    assert len(venv.bin_paths) == 1
+    assert venv.bin_paths[0] == venv.bin
+    assert str(dir_.joinpath("Scripts")) == venv.bin
+
+
 def test_create(
     monkeypatch: pytest.MonkeyPatch,
     make_one: Callable[..., tuple[VirtualEnv, Path]],