From 99766d99ed07cd00cd731ee6474845500dde0c96 Mon Sep 17 00:00:00 2001
From: Maxime U Garcia <max.u.garcia@gmail.com>
Date: Tue, 30 Jun 2026 15:37:59 +0200
Subject: [PATCH 01/27] Prepare dev (3.9.1dev) (#2225)

---
 .nf-core.yml              |   2 +-
 CHANGELOG.md              |  39 +++
 assets/multiqc_config.yml |   2 +-
 nextflow.config           |  57 +++-
 ro-crate-metadata.json    | 594 +++++++++++++++++++++++++++++---------
 5 files changed, 561 insertions(+), 133 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 5c329b8251..8431730ebe 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -24,4 +24,4 @@ template:
   name: sarek
   org: nf-core
   outdir: .
-  version: 3.9.0
+  version: 3.9.1dev
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3a284adc9e..88b429de3f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,6 +5,45 @@ All notable changes to this project will be documented in this file.
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
+## dev - unreleased
+
+### Added
+
+### Changed
+
+### Fixed
+
+### Removed
+
+### Dependencies - modules
+
+| Dependency | Old version | New version |
+| ---------- | ----------- | ----------- |
+
+### Dependencies - plugins
+
+| Dependency | Old version | New version |
+| ---------- | ----------- | ----------- |
+
+### Parameters
+
+| Params | status |
+| ------ | ------ |
+
+### Developer section
+
+#### Added
+
+- [#2225](https://github.com/nf-core/sarek/pull/2225) - Add contributor ORCIDs to `nextflow.config`
+
+#### Changed
+
+- [#2225](https://github.com/nf-core/sarek/pull/2225) - Back to dev (3.9.1dev)
+
+#### Fixed
+
+#### Removed
+
 ## [3.9.0](https://github.com/nf-core/sarek/releases/tag/3.9.0) - Sarvesjåhkå
 
 Sarvesjåhkå is the biggest stream from Sarvesvágge to flow in Rapaätno.
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 419eb35523..1ab45319b9 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -3,7 +3,7 @@ custom_logo_url: https://github.com/nf-core/sarek/
 custom_logo_title: "nf-core/sarek"
 
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/sarek/releases/tag/3.9.0" target="_blank">nf-core/sarek</a> analysis pipeline. For information about how to interpret these results, please see the <a href="https://nf-co.re/sarek/3.9.0/docs/output" target="_blank">documentation</a>.
+  This report has been generated by the <a href="https://github.com/nf-core/sarek/tree/dev" target="_blank">nf-core/sarek</a> analysis pipeline. For information about how to interpret these results, please see the <a href="https://nf-co.re/sarek/dev/docs/output" target="_blank">documentation</a>.
 report_section_order:
   "nf-core-sarek-methods-description":
     order: -1000
diff --git a/nextflow.config b/nextflow.config
index 06fb3810fd..f778ed037b 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -390,7 +390,7 @@ manifest {
     name            = 'nf-core/sarek'
     contributors    = [
         [
-            name: ' Friederike Hanssen',
+            name: 'Friederike Hanssen',
             affiliation: 'Seqera',
             email: 'friederike.hanssen@seqera.io',
             github: '@friederikehanssen',
@@ -415,78 +415,93 @@ manifest {
             name: 'Abhinav Sharma',
             github: '@abhi18av',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Adam Talbot',
             affiliation: 'Seqera',
             github: '@adamrtalbot',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Adrian Lärkeryd',
             github: '@adrlar',
             contribution: ['contributor'],
+            orcid: '0000-0003-1424-7840'
         ],
         [
             name: 'Àitor Olivares',
             github: '@AitorPeseta',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Alexander Peltzer',
             github: '@apeltzer',
             contribution: ['contributor'],
+            orcid: '0000-0002-6503-2180'
         ],
         [
             name: 'Alison Meynert',
             github: '@ameynert',
             contribution: ['contributor'],
+            orcid: '0000-0001-5839-1751'
         ],
         [
             name: 'Anders Sune Pedersen',
             github: '@asp8200',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Aron Skaftason',
             github: '@arontommi',
             contribution: ['contributor'],
+            orcid: '0000-0002-5547-8568'
         ],
         [
             name: 'Barry Digby',
             github: '@BarryDigby',
             contribution: ['contributor'],
+            orcid: '0000-0002-8492-6585'
         ],
         [
             name: 'Bekir Ergüner',
             github: '@berguner',
             contribution: ['contributor'],
+            orcid: '0000-0001-5475-0892'
         ],
         [
             name: 'Björn Nystedt',
             github: '@bjornnystedt',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Christina Chatzipantsiou',
             github: '@cgpu',
             contribution: ['contributor'],
+            orcid: '0000-0002-4257-7241'
         ],
         [
             name: 'Chela James',
             github: '@chelauk',
             contribution: ['contributor'],
+            orcid: '0000-0003-3996-0909'
         ],
         [
             name: 'David Mas-Ponte',
             github: '@davidmasp',
             contribution: ['contributor'],
+            orcid: '0000-0001-7409-305X'
         ],
         [
             name: 'Edmund Miller',
             affiliation: 'Seqera',
             github: '@edmundmiller',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Famke Bäuerle',
@@ -499,57 +514,68 @@ manifest {
             name: 'Francesco Lescai',
             github: '@lescai',
             contribution: ['contributor'],
+            orcid: '0000-0002-6399-9101'
         ],
         [
             name: 'Francisco Martínez',
             github: '@nevinwu',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Gavin Mackenzie',
             github: '@GCJMackenzie',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Gisela Gabernet',
             github: '@ggabernet',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Grant Neilson',
             github: '@grantn5',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Max Käller',
             github: '@gulfshores',
             contribution: ['contributor'],
+            orcid: '0000-0001-6813-3051'
         ],
         [
             name: 'Harshil Patel',
             affiliation: 'Seqera',
             github: '@drpatelh',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Hongwei Ye',
             github: '@YeHW',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'James A. Fellows Yates',
             github: '@jfy133',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Jesper Eisfeldt',
             github: '@J35P312',
             contribution: ['contributor'],
+            orcid: '0000-0003-3716-4917'
         ],
         [
             name: 'Johannes Alneberg',
             github: '@alneberg',
             contribution: ['contributor'],
+            orcid: '0000-0002-2467-008X'
         ],
         [
             name: 'Jonas Kjellin',
@@ -562,113 +588,135 @@ manifest {
             name: 'José Fernández Navarro',
             github: '@jfnavarro',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Júlia Mir Pedrol',
             github: '@mirpedrol',
             contribution: ['contributor'],
+            orcid: '0000-0001-6104-9260'
         ],
         [
             name: 'Ken Brewer',
             affiliation: 'Seqera',
             github: '@kenibrewer',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Lasse Westergaard Folkersen',
             github: '@lassefolkersen',
             contribution: ['contributor'],
+            orcid: '0000-0003-0708-9530'
         ],
         [
             name: 'Lucia Conde',
             github: '@lconde-ucl',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Louis Le Nézet',
             github: '@LouisLeNezet',
             contribution: ['contributor'],
+            orcid: '0009-0000-0202-2703'
         ],
         [
             name: 'Malin Larsson',
             github: '@malinlarsson',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Marcel Martin',
             github: '@marcelm',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Nick Smith',
             github: '@nickhsmith',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Nicolas Schcolnicov',
             github: '@nschcolnicov',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Nilesh Tawari',
             github: '@nilesh-tawari',
             contribution: ['contributor'],
+            orcid: '0000-0002-1127-0765'
         ],
         [
             name: 'Nils Homer',
             github: '@nh13',
             contribution: ['contributor'],
+            orcid: '0009-0007-7860-1155'
         ],
         [
             name: 'Olga Botvinnik',
             github: '@olgabot',
             contribution: ['contributor'],
+            orcid: '0000-0003-4412-7970'
         ],
         [
             name: 'Oskar Wacker',
             github: '@WackerO',
             contribution: ['contributor'],
+            orcid: '0009-0003-2041-610X'
         ],
         [
             name: 'Pall Olason',
             github: '@pallolason',
             contribution: ['contributor'],
+            orcid: '0000-0002-3350-3443'
         ],
         [
             name: 'Paul Cantalupo',
             github: '@pcantalupo',
             contribution: ['contributor'],
+            orcid: '0000-0003-3966-8481'
         ],
         [
             name: 'Phil Ewels',
             affiliation: 'Seqera',
             github: '@ewels',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Pierre Lindenbaum',
             github: '@lindenb',
             contribution: ['contributor'],
+            orcid: '0000-0003-0148-9787'
         ],
         [
             name: 'Sabrina Krakau',
             github: '@skrakau',
             contribution: ['contributor'],
+            orcid: '0000-0003-0603-7907'
         ],
         [
             name: 'Sam Minot',
             github: '@sminot',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Sebastian DiLorenzo',
             github: '@Sebastian-D',
             contribution: ['contributor'],
+            orcid: '0000-0002-9759-2211'
         ],
         [
             name: 'Silvia Morini',
             github: '@silviamorins',
             contribution: ['contributor'],
+            orcid: '0000-0002-2798-9870'
         ],
         [
             name: 'Simon Pearce',
@@ -681,26 +729,31 @@ manifest {
             name: 'Solenne Correard',
             github: '@scorreard',
             contribution: ['contributor'],
+            orcid: '0000-0002-0554-5443'
         ],
         [
             name: 'Susanne Jodoin',
             github: '@SusiJo',
             contribution: ['contributor'],
+            orcid: '0009-0001-9152-7291'
         ],
         [
             name: 'Tobias Koch',
             github: '@KochTobi',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Winni Kretzschmar',
             github: '@winni2k',
             contribution: ['contributor'],
+            orcid: ''
         ],
         [
             name: 'Patricie Skaláková',
             github: '@Patricie34',
             contribution: ['contributor'],
+            orcid: '0009-0009-0647-7639'
         ]
     ]
     homePage        = 'https://github.com/nf-core/sarek'
@@ -709,7 +762,7 @@ manifest {
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
     nextflowVersion = '!>=25.10.2'
-    version         = '3.9.0'
+    version         = '3.9.1dev'
     doi             = '10.12688/f1000research.16665.2, 10.1093/nargab/lqae031, 10.5281/zenodo.3476425'
 }
 
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index e6fbb45a6a..1b96b6ccf1 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -21,9 +21,9 @@
         {
             "@id": "./",
             "@type": "Dataset",
-            "creativeWorkStatus": "Stable",
-            "datePublished": "2026-06-01T12:50:07+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway.png\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen and Maxime U Garcia (now at [Seqera](https://seqera.io))\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek) (you can join with [this invite](https://nf-co.re/join/slack)), or contact us: [Maxime U Garcia](mailto:maxime.garcia@seqera.io?subject=[GitHub]%20nf-core/sarek), [Friederike Hanssen](mailto:friederike.hanssen@qbic.uni-tuebingen.de?subject=[GitHub]%20nf-core/sarek)\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
+            "creativeWorkStatus": "InProgress",
+            "datePublished": "2026-06-30T11:52:57+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen and Maxime U Garcia (now at [Seqera](https://seqera.io))\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek) (you can join with [this invite](https://nf-co.re/join/slack)), or contact us: [Maxime U Garcia](mailto:maxime.garcia@seqera.io?subject=[GitHub]%20nf-core/sarek), [Friederike Hanssen](mailto:friederike.hanssen@qbic.uni-tuebingen.de?subject=[GitHub]%20nf-core/sarek)\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
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+            "name": "Anders Sune Pedersen",
+            "url": "https://github.com/asp8200"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-5547-8568",
+            "@type": "Person",
+            "name": "Aron Skaftason",
+            "url": "https://github.com/arontommi"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-8492-6585",
+            "@type": "Person",
+            "name": "Barry Digby",
+            "url": "https://github.com/BarryDigby"
+        },
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+            "@type": "Person",
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+            "url": "https://github.com/berguner"
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+        },
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         },
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             "@type": "Person",
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+            "url": "https://github.com/davidmasp"
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+            "name": "Edmund Miller",
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+            "email": "45968370+famosab@users.noreply.github.com",
+            "name": "Famke Bäuerle",
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+            "name": "Harshil Patel",
+            "url": "https://github.com/drpatelh"
+        },
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+            "url": "https://github.com/YeHW"
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+        {
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+        },
+        {
+            "@id": "https://orcid.org/0000-0002-2467-008X",
+            "@type": "Person",
+            "email": "johannes.alneberg@scilifelab.se",
+            "name": "Johannes Alneberg",
+            "url": "https://github.com/alneberg"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-3830-7046",
+            "@type": "Person",
+            "affiliation": "National Genomics Infrastructure (NGI), Uppsala University, Sweden",
+            "email": "jonas.kjellin@gmail.com",
+            "name": "Jonas Kjellin",
+            "url": "https://github.com/kjellinjonas"
+        },
+        {
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+            "name": "José Fernández Navarro",
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         {
             "@id": "https://orcid.org/0000-0001-6104-9260",
             "@type": "Person",
             "email": "mirp.julia@gmail.com",
-            "name": "Júlia Mir Pedrol"
+            "name": "Júlia Mir Pedrol",
+            "url": "https://github.com/mirpedrol"
         },
         {
-            "@id": "#l.conde@ucl.ac.uk",
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+            "affiliation": "Seqera",
+            "email": "kenibrewer@users.noreply.github.com",
+            "name": "Ken Brewer",
+            "url": "https://github.com/kenibrewer"
+        },
+        {
+            "@id": "https://orcid.org/0000-0003-0708-9530",
+            "@type": "Person",
+            "name": "Lasse Westergaard Folkersen",
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             "@type": "Person",
             "email": "l.conde@ucl.ac.uk",
-            "name": "Lucia Conde"
+            "name": "Lucia Conde",
+            "url": "https://github.com/lconde-ucl"
         },
         {
-            "@id": "https://orcid.org/0000-0003-3996-0909",
+            "@id": "https://orcid.org/0009-0000-0202-2703",
             "@type": "Person",
-            "email": "chela.james@icr.ac.uk",
-            "name": "chela james"
+            "name": "Louis Le Nézet",
+            "url": "https://github.com/LouisLeNezet"
         },
         {
-            "@id": "#yehwhey@gmail.com",
+            "@id": "#ba7614f8-0190-4209-a2f4-a8a8dd8f96bf",
             "@type": "Person",
-            "email": "yehwhey@gmail.com",
-            "name": "Hongwei Ye"
+            "email": "malin.larsson@liu.se",
+            "name": "Malin Larsson",
+            "url": "https://github.com/malinlarsson"
         },
         {
-            "@id": "#famke.baeuerle@gmail.com",
+            "@id": "#e9bfeffd-dc8a-42f6-a3e0-5ebcb00c3097",
             "@type": "Person",
-            "email": "famke.baeuerle@gmail.com",
-            "name": "Famke Bäuerle"
+            "name": "Marcel Martin",
+            "url": "https://github.com/marcelm"
         },
         {
-            "@id": "#jc.fernandez.navarro@gmail.com",
+            "@id": "#093c6988-c462-4474-b731-a9a085033797",
             "@type": "Person",
-            "email": "jc.fernandez.navarro@gmail.com",
-            "name": "José Fernández Navarro"
+            "name": "Nick Smith",
+            "url": "https://github.com/nickhsmith"
         },
         {
-            "@id": "https://orcid.org/0000-0003-3966-8481",
+            "@id": "#b9b9b14b-4d16-4cc2-8689-c2abac06db13",
             "@type": "Person",
-            "email": "pcantalupo@gmail.com",
-            "name": "Paul Cantalupo"
+            "email": "90359308+nschcolnicov@users.noreply.github.com",
+            "name": "Nicolas Schcolnicov",
+            "url": "https://github.com/nschcolnicov"
         },
         {
-            "@id": "#24893913+SPPearce@users.noreply.github.com",
+            "@id": "https://orcid.org/0000-0002-1127-0765",
             "@type": "Person",
-            "email": "24893913+SPPearce@users.noreply.github.com",
-            "name": "Simon Pearce"
+            "name": "Nilesh Tawari",
+            "url": "https://github.com/nilesh-tawari"
         },
         {
-            "@id": "#heuermh@acm.org",
+            "@id": "https://orcid.org/0009-0007-7860-1155",
             "@type": "Person",
-            "email": "heuermh@acm.org",
-            "name": "Michael L Heuer"
+            "email": "nh13@users.noreply.github.com",
+            "name": "Nils Homer",
+            "url": "https://github.com/nh13"
         },
         {
-            "@id": "https://orcid.org/0000-0003-1387-0251",
+            "@id": "https://orcid.org/0000-0003-4412-7970",
             "@type": "Person",
-            "email": "45968370+famosab@users.noreply.github.com",
-            "name": "Famke Bäuerle"
+            "email": "olga.botvinnik@gmail.com",
+            "name": "Olga Botvinnik",
+            "url": "https://github.com/olgabot"
         },
         {
-            "@id": "https://orcid.org/0000-0001-6280-4643",
+            "@id": "https://orcid.org/0009-0003-2041-610X",
             "@type": "Person",
-            "email": "szilveszter.juhos@scilifelab.se",
-            "name": "Szilveszter Juhos"
+            "name": "Oskar Wacker",
+            "url": "https://github.com/WackerO"
+        },
+        {
+            "@id": "https://orcid.org/0000-0002-3350-3443",
+            "@type": "Person",
+            "name": "Pall Olason",
+            "url": "https://github.com/pallolason"
+        },
+        {
+            "@id": "https://orcid.org/0000-0003-3966-8481",
+            "@type": "Person",
+            "email": "pcantalupo@gmail.com",
+            "name": "Paul Cantalupo",
+            "url": "https://github.com/pcantalupo"
+        },
+        {
+            "@id": "#14924136-a73b-406c-91eb-5f10620ea097",
+            "@type": "Person",
+            "affiliation": "Seqera",
+            "email": "phil.ewels@scilifelab.se",
+            "name": "Phil Ewels",
+            "url": "https://github.com/ewels"
+        },
+        {
+            "@id": "https://orcid.org/0000-0003-0148-9787",
+            "@type": "Person",
+            "email": "33838+lindenb@users.noreply.github.com",
+            "name": "Pierre Lindenbaum",
+            "url": "https://github.com/lindenb"
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         {
             "@id": "https://orcid.org/0000-0003-0603-7907",
             "@type": "Person",
             "email": "sabrina.krakau.qbic@gmail.com",
-            "name": "Sabrina Krakau"
+            "name": "Sabrina Krakau",
+            "url": "https://github.com/skrakau"
         },
         {
-            "@id": "#53608000+lescai@users.noreply.github.com",
+            "@id": "#1724fe39-c41b-49b9-8525-d7df86b20b98",
             "@type": "Person",
-            "email": "53608000+lescai@users.noreply.github.com",
-            "name": "Francesco L"
+            "email": "sminot@gmail.com",
+            "name": "Sam Minot",
+            "url": "https://github.com/sminot"
         },
         {
-            "@id": "https://orcid.org/0000-0002-6503-2180",
+            "@id": "https://orcid.org/0000-0002-9759-2211",
             "@type": "Person",
-            "email": "apeltzer@users.noreply.github.com",
-            "name": "Alexander Peltzer"
+            "name": "Sebastian DiLorenzo",
+            "url": "https://github.com/Sebastian-D"
         },
         {
-            "@id": "#adr.lar@me.com",
+            "@id": "https://orcid.org/0000-0002-2798-9870",
             "@type": "Person",
-            "email": "adr.lar@me.com",
-            "name": "Adrian Larkeryd"
+            "name": "Silvia Morini",
+            "url": "https://github.com/silviamorins"
         },
         {
-            "@id": "https://orcid.org/0009-0001-9875-5262",
+            "@id": "https://orcid.org/0000-0002-1680-5538",
             "@type": "Person",
-            "email": "friederike.hanssen@seqera.io",
-            "name": "Friederike Hanssen"
+            "affiliation": "NeoGenomics Laboratories",
+            "email": "24893913+SPPearce@users.noreply.github.com",
+            "name": "Simon Pearce",
+            "url": "https://github.com/SPPearce"
         },
         {
-            "@id": "https://orcid.org/0009-0007-7860-1155",
+            "@id": "https://orcid.org/0000-0002-0554-5443",
             "@type": "Person",
-            "email": "nh13@users.noreply.github.com",
-            "name": "Nils Homer"
+            "name": "Solenne Correard",
+            "url": "https://github.com/scorreard"
+        },
+        {
+            "@id": "https://orcid.org/0009-0001-9152-7291",
+            "@type": "Person",
+            "name": "Susanne Jodoin",
+            "url": "https://github.com/SusiJo"
+        },
+        {
+            "@id": "#08187f11-d51b-40f0-b09d-a1ff8bf52d45",
+            "@type": "Person",
+            "name": "Tobias Koch",
+            "url": "https://github.com/KochTobi"
+        },
+        {
+            "@id": "#30622e12-b2e6-43e8-99ed-b07a5cf40165",
+            "@type": "Person",
+            "name": "Winni Kretzschmar",
+            "url": "https://github.com/winni2k"
         },
         {
-            "@id": "https://orcid.org/0000-0002-5762-6253",
+            "@id": "https://orcid.org/0009-0009-0647-7639",
             "@type": "Person",
-            "email": "bounlu@gmail.com",
-            "name": "Ömer An"
+            "email": "158295213+Patricie34@users.noreply.github.com",
+            "name": "Patricie Skaláková",
+            "url": "https://github.com/Patricie34"
         }
     ]
 }
\ No newline at end of file

From e219d7bcd6357ea811997b33222fe00b7ddea0b5 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Famke=20B=C3=A4uerle?=
 <45968370+famosab@users.noreply.github.com>
Date: Mon, 6 Jul 2026 10:39:47 +0200
Subject: [PATCH 02/27] Add varlociraptor/filterfdr, update varlociraptor
 (#2208)

<!--
# nf-core/sarek pull request

Many thanks for contributing to nf-core/sarek!

Please fill in the appropriate checklist below (delete whatever is not
relevant).
These are the most common things requested on pull requests (PRs).

Remember that PRs should be made against the dev branch, unless you're
preparing a pipeline release.

Learn more about contributing:
[CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
-->

## PR checklist

- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [ ] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [x] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [x] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).
---
 CHANGELOG.md                                  | 17 ++++-
 conf/modules/freebayes.config                 |  3 +-
 conf/modules/varlociraptor.config             | 27 ++++++-
 docs/usage.md                                 |  4 +-
 main.nf                                       |  5 ++
 modules.json                                  | 11 ++-
 .../callvariants/environment.yml              |  2 +-
 .../varlociraptor/callvariants/main.nf        |  6 +-
 .../environment.yml                           |  2 +-
 .../estimatealignmentproperties/main.nf       |  6 +-
 .../varlociraptor/filterfdr/environment.yml   |  8 ++
 .../nf-core/varlociraptor/filterfdr/main.nf   | 40 ++++++++++
 .../nf-core/varlociraptor/filterfdr/meta.yml  | 76 +++++++++++++++++++
 .../varlociraptor/preprocess/environment.yml  |  2 +-
 .../nf-core/varlociraptor/preprocess/main.nf  |  6 +-
 nextflow.config                               |  4 +
 nextflow_schema.json                          | 20 +++++
 .../local/post_variantcalling/main.nf         | 26 ++++---
 .../local/vcf_varlociraptor_single/main.nf    |  9 ++-
 .../local/vcf_varlociraptor_somatic/main.nf   |  9 ++-
 tests/postprocess_varlociraptor.nf.test.snap  | 57 ++++++++------
 tests/variant_calling_all.nf.test.snap        | 20 ++---
 tests/variant_calling_freebayes.nf.test.snap  | 34 ++++-----
 workflows/sarek/main.nf                       | 11 ++-
 24 files changed, 315 insertions(+), 90 deletions(-)
 create mode 100644 modules/nf-core/varlociraptor/filterfdr/environment.yml
 create mode 100644 modules/nf-core/varlociraptor/filterfdr/main.nf
 create mode 100644 modules/nf-core/varlociraptor/filterfdr/meta.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 88b429de3f..37b5bdc04d 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,6 +9,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Added
 
+- [#2208](https://github.com/nf-core/sarek/pull/2208) - Add varlociraptor/filterfdr
+
 ### Changed
 
 ### Fixed
@@ -17,8 +19,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Dependencies - modules
 
-| Dependency | Old version | New version |
-| ---------- | ----------- | ----------- |
+| Dependency    | Old version | New version |
+| ------------- | ----------- | ----------- |
+| varlociraptor | 8.9.3       | 8.9.5       |
 
 ### Dependencies - plugins
 
@@ -27,8 +30,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Parameters
 
-| Params | status |
-| ------ | ------ |
+| Params                              | status |
+| ----------------------------------- | ------ |
+| `--varlociraptor_events_germline`   | New    |
+| `--varlociraptor_events_somatic,`   | New    |
+| `--varlociraptor_events_tumor_only` | New    |
+| `--varlociraptor_fdr`               | New    |
 
 ### Developer section
 
@@ -42,6 +49,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 #### Fixed
 
+- [#2208](https://github.com/nf-core/sarek/pull/2208) - Update freebayes params (remove `--pooled-discrete` and change `--min-alternate-fraction` from 0.03 to 0.01), move chunk_size param
+
 #### Removed
 
 ## [3.9.0](https://github.com/nf-core/sarek/releases/tag/3.9.0) - Sarvesjåhkå
diff --git a/conf/modules/freebayes.config b/conf/modules/freebayes.config
index 1fcfc7de9b..7a1ec2a1c5 100644
--- a/conf/modules/freebayes.config
+++ b/conf/modules/freebayes.config
@@ -73,11 +73,10 @@ process {
     // PAIR_VARIANT_CALLING
         withName: '.*:BAM_VARIANT_CALLING_SOMATIC_ALL:BAM_VARIANT_CALLING_FREEBAYES:FREEBAYES' {
             ext.args = { "--pooled-continuous \
-                            --pooled-discrete \
                             --genotype-qualities \
                             --report-genotype-likelihood-max \
                             --allele-balance-priors-off \
-                            --min-alternate-fraction 0.03 \
+                            --min-alternate-fraction 0.01 \
                             --min-repeat-entropy 1 \
                             --min-alternate-count 2 " }
         }
diff --git a/conf/modules/varlociraptor.config b/conf/modules/varlociraptor.config
index 029fb40fde..575a2bf336 100644
--- a/conf/modules/varlociraptor.config
+++ b/conf/modules/varlociraptor.config
@@ -76,7 +76,14 @@ process {
 
     withName: '.*:VCF_VARLOCIRAPTOR_GERMLINE:CONCAT_CALLED_CHUNKS' {
         ext.prefix = { "${meta.id}.${meta.variantcaller}.germline.varlociraptor.concat" }
-        ext.args   = { '--allow-overlaps --output-type z' }
+        ext.args   = { '--allow-overlaps --output-type b' }
+        publishDir = [
+            enabled: false
+        ]
+    }
+
+    withName: '.*:VCF_VARLOCIRAPTOR_GERMLINE:VARLOCIRAPTOR_FILTERFDR' {
+        ext.prefix = { "${meta.id}.${meta.variantcaller}.germline.varlociraptor.filtered" }
         publishDir = [
             enabled: false
         ]
@@ -94,7 +101,14 @@ process {
 
     withName: '.*:VCF_VARLOCIRAPTOR_TUMOR_ONLY:CONCAT_CALLED_CHUNKS' {
         ext.prefix = { "${meta.id}.${meta.variantcaller}.tumor_only.varlociraptor.concat" }
-        ext.args   = { '--allow-overlaps --output-type z' }
+        ext.args   = { '--allow-overlaps --output-type b' }
+        publishDir = [
+            enabled: false
+        ]
+    }
+
+    withName: '.*:VCF_VARLOCIRAPTOR_TUMOR_ONLY:VARLOCIRAPTOR_FILTERFDR' {
+        ext.prefix = { "${meta.id}.${meta.variantcaller}.tumor_only.varlociraptor.filtered" }
         publishDir = [
             enabled: false
         ]
@@ -189,7 +203,14 @@ process {
 
     withName: '.*:VCF_VARLOCIRAPTOR_SOMATIC:CONCAT_CALLED_CHUNKS' {
         ext.prefix = { "${meta.id}.${meta.variantcaller}.somatic.varlociraptor.concat" }
-        ext.args   = { '--allow-overlaps --output-type z' }
+        ext.args   = { '--allow-overlaps --output-type b' }
+        publishDir = [
+            enabled: false
+        ]
+    }
+
+    withName: '.*:VCF_VARLOCIRAPTOR_SOMATIC:VARLOCIRAPTOR_FILTERFDR' {
+        ext.prefix = { "${meta.id}.${meta.variantcaller}.somatic.varlociraptor.filtered" }
         publishDir = [
             enabled: false
         ]
diff --git a/docs/usage.md b/docs/usage.md
index cab540e8b5..3748327f43 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -652,7 +652,7 @@ This list is by no means exhaustive and it will depend on the specific analysis
 | [ASCAT](https://github.com/VanLoo-lab/ascat)                                                            |  x  |  x  |   -    |     -     |     -      |           x            |
 | [CNVKit](https://cnvkit.readthedocs.io/en/stable/)                                                      |  x  |  x  |   -    |     x     |     x      |           x            |
 | [Control-FREEC](https://github.com/BoevaLab/FREEC)                                                      |  x  |  x  |   x    |     -     |     x      |           x            |
-| [MSIsensor2](https://github.com/niu-lab/msisensor2)                                                     |  x  |  x  |   x    |     -     |     X      |           -            |
+| [MSIsensor2](https://github.com/niu-lab/msisensor2)                                                     |  x  |  x  |   x    |     -     |     x      |           -            |
 | [MSIsensorPro](https://github.com/xjtu-omics/msisensor-pro)                                             |  x  |  x  |   x    |     -     |     -      |           x            |
 | [Varlociraptor](https://varlociraptor.github.io/landing/)                                               |  x  |  x  |   x    |     x     |     x      |           x            |
 
@@ -904,6 +904,8 @@ Varlociraptor allows the usage of different scenario files, a few examples can b
 
 You can control the number of chunks that the candidate VCF file is split into by `--varlociraptor_chunk_size <integer>`, it is set to reasonable default (15) but more chunks might aid in accelerating your workflow run if you can run more processes in parallel.
 
+Varlociraptor by default just returns all candidate variants annotated with probabilities. The variants need to be filtered to yield a representativ result, see the [docs](https://varlociraptor.github.io/docs/filtering/) for more info. In sarek this is implemented with default set events related to the scenario files which used by default. If you use your own scenario file please adapt the events accordingly by using the flags `--varlociraptor_events_tumor_only`, `--varlociraptor_events_somatic` and `--varlociraptor_events_germline`. The events need to be supplied as a list with spaces between the events. Filtering also depends on the False Discovery Rate (FDR) in sarek we opted for a default of filtering in `--mode local-smart` to a FDR of 5% with `--fdr 0.05`. The FDR can be controlled by `--varlociraptor_fdr`.
+
 ## Spark related issues
 
 If you have problems running processes that make use of Spark such as `MarkDuplicates`.
diff --git a/main.nf b/main.nf
index 679b530688..7506eec94a 100755
--- a/main.nf
+++ b/main.nf
@@ -322,6 +322,11 @@ workflow NFCORE_SAREK {
         PREPARE_GENOME.out.pon,
         PREPARE_GENOME.out.pon_tbi,
         params.sentieon_dnascope_model ? channel.fromPath(params.sentieon_dnascope_model).collect() : channel.value([]),
+        params.varlociraptor_chunk_size,
+        params.varlociraptor_events_germline,
+        params.varlociraptor_events_somatic,
+        params.varlociraptor_events_tumor_only,
+        params.varlociraptor_fdr,
         params.varlociraptor_scenario_germline ? channel.fromPath(params.varlociraptor_scenario_germline).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect(),
         params.varlociraptor_scenario_somatic ? channel.fromPath(params.varlociraptor_scenario_somatic).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect(),
         params.varlociraptor_scenario_tumor_only ? channel.fromPath(params.varlociraptor_scenario_tumor_only).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect(),
diff --git a/modules.json b/modules.json
index 9571d81942..ca3721a53e 100644
--- a/modules.json
+++ b/modules.json
@@ -573,17 +573,22 @@
                     },
                     "varlociraptor/callvariants": {
                         "branch": "master",
-                        "git_sha": "5ae3c74e09c529c5c768aa89397e490ba4728219",
+                        "git_sha": "289374af2f576e1040db10c42759c4a4804ffeb5",
                         "installed_by": ["modules"]
                     },
                     "varlociraptor/estimatealignmentproperties": {
                         "branch": "master",
-                        "git_sha": "5ae3c74e09c529c5c768aa89397e490ba4728219",
+                        "git_sha": "289374af2f576e1040db10c42759c4a4804ffeb5",
+                        "installed_by": ["modules"]
+                    },
+                    "varlociraptor/filterfdr": {
+                        "branch": "master",
+                        "git_sha": "289374af2f576e1040db10c42759c4a4804ffeb5",
                         "installed_by": ["modules"]
                     },
                     "varlociraptor/preprocess": {
                         "branch": "master",
-                        "git_sha": "5ae3c74e09c529c5c768aa89397e490ba4728219",
+                        "git_sha": "289374af2f576e1040db10c42759c4a4804ffeb5",
                         "installed_by": ["modules"]
                     },
                     "vcflib/vcffilter": {
diff --git a/modules/nf-core/varlociraptor/callvariants/environment.yml b/modules/nf-core/varlociraptor/callvariants/environment.yml
index 4cfd4a7d5a..107e4ae225 100644
--- a/modules/nf-core/varlociraptor/callvariants/environment.yml
+++ b/modules/nf-core/varlociraptor/callvariants/environment.yml
@@ -5,4 +5,4 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/varlociraptor
-  - bioconda::varlociraptor=8.9.3
+  - bioconda::varlociraptor=8.9.5
diff --git a/modules/nf-core/varlociraptor/callvariants/main.nf b/modules/nf-core/varlociraptor/callvariants/main.nf
index fc24cb5ab4..0799717231 100644
--- a/modules/nf-core/varlociraptor/callvariants/main.nf
+++ b/modules/nf-core/varlociraptor/callvariants/main.nf
@@ -3,9 +3,9 @@ process VARLOCIRAPTOR_CALLVARIANTS {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9a/9ac0825c21b2cbaf9535ffe443e53a0bb4d61596cafcb5a5b444dfb31b945ab2/data'
-        : 'community.wave.seqera.io/library/varlociraptor:8.9.3--fa2ce5da2782669c'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/varlociraptor%3A8.9.5--h24073b4_0'
+        : 'quay.io/biocontainers/varlociraptor:8.9.5--h24073b4_0'}"
 
     input:
     tuple val(meta), path(vcfs), path(scenario), val(scenario_aliases)
diff --git a/modules/nf-core/varlociraptor/estimatealignmentproperties/environment.yml b/modules/nf-core/varlociraptor/estimatealignmentproperties/environment.yml
index 4cfd4a7d5a..107e4ae225 100644
--- a/modules/nf-core/varlociraptor/estimatealignmentproperties/environment.yml
+++ b/modules/nf-core/varlociraptor/estimatealignmentproperties/environment.yml
@@ -5,4 +5,4 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/varlociraptor
-  - bioconda::varlociraptor=8.9.3
+  - bioconda::varlociraptor=8.9.5
diff --git a/modules/nf-core/varlociraptor/estimatealignmentproperties/main.nf b/modules/nf-core/varlociraptor/estimatealignmentproperties/main.nf
index ea786c54b9..3a52b178ee 100644
--- a/modules/nf-core/varlociraptor/estimatealignmentproperties/main.nf
+++ b/modules/nf-core/varlociraptor/estimatealignmentproperties/main.nf
@@ -3,9 +3,9 @@ process VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9a/9ac0825c21b2cbaf9535ffe443e53a0bb4d61596cafcb5a5b444dfb31b945ab2/data'
-        : 'community.wave.seqera.io/library/varlociraptor:8.9.3--fa2ce5da2782669c'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/varlociraptor%3A8.9.5--h24073b4_0'
+        : 'quay.io/biocontainers/varlociraptor:8.9.5--h24073b4_0'}"
 
     input:
     tuple val(meta), path(bam), path(bai), path(fasta), path(fai)
diff --git a/modules/nf-core/varlociraptor/filterfdr/environment.yml b/modules/nf-core/varlociraptor/filterfdr/environment.yml
new file mode 100644
index 0000000000..107e4ae225
--- /dev/null
+++ b/modules/nf-core/varlociraptor/filterfdr/environment.yml
@@ -0,0 +1,8 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  # renovate: datasource=conda depName=bioconda/varlociraptor
+  - bioconda::varlociraptor=8.9.5
diff --git a/modules/nf-core/varlociraptor/filterfdr/main.nf b/modules/nf-core/varlociraptor/filterfdr/main.nf
new file mode 100644
index 0000000000..0f8359511a
--- /dev/null
+++ b/modules/nf-core/varlociraptor/filterfdr/main.nf
@@ -0,0 +1,40 @@
+process VARLOCIRAPTOR_FILTERFDR {
+    tag "${meta.id}"
+    label 'process_single'
+
+    conda "${moduleDir}/environment.yml"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/varlociraptor%3A8.9.5--h24073b4_0'
+        : 'quay.io/biocontainers/varlociraptor:8.9.5--h24073b4_0'}"
+
+    input:
+    tuple val(meta), path(vcf), val(events), val(fdr)
+
+    output:
+    tuple val(meta), path("*.bcf"), emit: bcf
+    tuple val("${task.process}"), val('varlociraptor'), eval("varlociraptor --version | sed 's/^varlociraptor //'"), topic: versions, emit: versions_varlociraptor
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}.fdr-controlled"
+    def mode = args.contains("--mode global-smart") ? "global-smart" : "local-smart"
+    def args_corrected = args.replace('--mode global-smart', '').replace('--mode local-smart', '').trim()
+    """
+    varlociraptor filter-calls control-fdr \\
+        --mode ${mode} \\
+        ${vcf} \\
+        --events ${events} \\
+        --fdr ${fdr} \\
+        ${args_corrected} \\
+        > ${prefix}.bcf
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}.fdr-controlled"
+    """
+    touch ${prefix}.bcf
+    """
+}
diff --git a/modules/nf-core/varlociraptor/filterfdr/meta.yml b/modules/nf-core/varlociraptor/filterfdr/meta.yml
new file mode 100644
index 0000000000..51d59c1431
--- /dev/null
+++ b/modules/nf-core/varlociraptor/filterfdr/meta.yml
@@ -0,0 +1,76 @@
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json
+name: "varlociraptor_filterfdr"
+description: Filter Varlociraptor variant calls using FDR control.
+keywords:
+  - varlociraptor
+  - vcf
+  - bcf
+  - fdr
+  - variant filtering
+tools:
+  - "varlociraptor":
+      description: "Flexible, uncertainty-aware variant calling with parameter free
+        filtration via FDR control."
+      homepage: "https://varlociraptor.github.io/docs/estimating/"
+      documentation: "https://varlociraptor.github.io/docs/estimating/"
+      tool_dev_url: "https://github.com/varlociraptor/varlociraptor"
+      doi: "10.1186/s13059-020-01993-6"
+      licence: ["GPL v3"]
+      identifier: biotools:varlociraptor
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information.
+          e.g. `[ id:'sample1' ]`
+    - vcf:
+        type: file
+        description: Variant calls to be filtered.
+        pattern: "*.{vcf,vcf.gz,bcf}"
+        ontologies:
+          - edam: "http://edamontology.org/format_3016" # VCF
+          - edam: "http://edamontology.org/format_2572" # BCF
+    - events:
+        type: string
+        description: Event(s) of interest passed to `--events`.
+    - fdr:
+        type: float
+        description: False discovery rate threshold passed to `--fdr`.
+output:
+  bcf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*.bcf":
+          type: file
+          description: BCF file containing fdr-controlled calls
+          pattern: "*.bcf"
+          ontologies:
+            - edam: http://edamontology.org/format_3020 # BCF
+  versions_varlociraptor:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - varlociraptor:
+          type: string
+          description: The tool name
+      - "varlociraptor --version | sed 's/^varlociraptor //'":
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - varlociraptor:
+          type: string
+          description: The tool name
+      - "varlociraptor --version | sed 's/^varlociraptor //'":
+          type: eval
+          description: The command used to generate the version of the tool
+authors:
+  - "@famosab"
+maintainers:
+  - "@famosab"
diff --git a/modules/nf-core/varlociraptor/preprocess/environment.yml b/modules/nf-core/varlociraptor/preprocess/environment.yml
index 4cfd4a7d5a..107e4ae225 100644
--- a/modules/nf-core/varlociraptor/preprocess/environment.yml
+++ b/modules/nf-core/varlociraptor/preprocess/environment.yml
@@ -5,4 +5,4 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/varlociraptor
-  - bioconda::varlociraptor=8.9.3
+  - bioconda::varlociraptor=8.9.5
diff --git a/modules/nf-core/varlociraptor/preprocess/main.nf b/modules/nf-core/varlociraptor/preprocess/main.nf
index 4fabdbfdf1..5d71b94b86 100644
--- a/modules/nf-core/varlociraptor/preprocess/main.nf
+++ b/modules/nf-core/varlociraptor/preprocess/main.nf
@@ -3,9 +3,9 @@ process VARLOCIRAPTOR_PREPROCESS {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9a/9ac0825c21b2cbaf9535ffe443e53a0bb4d61596cafcb5a5b444dfb31b945ab2/data'
-        : 'community.wave.seqera.io/library/varlociraptor:8.9.3--fa2ce5da2782669c'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/varlociraptor%3A8.9.5--h24073b4_0'
+        : 'quay.io/biocontainers/varlociraptor:8.9.5--h24073b4_0'}"
 
     input:
     tuple val(meta), path(bam), path(bai), path(candidates), path(alignment_json), path(fasta), path(fai)
diff --git a/nextflow.config b/nextflow.config
index f778ed037b..d8e204796f 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -94,6 +94,10 @@ params {
 
     // Post variant calling
     varlociraptor_chunk_size          = 15    // default chunk size for Varlociraptor
+    varlociraptor_events_tumor_only   = "somatic_tumor_high somatic_tumor_low" // uses default events defined in used scenario file
+    varlociraptor_events_somatic      = "somatic_tumor_high somatic_tumor_low" // uses default events defined in used scenario file
+    varlociraptor_events_germline     = "germline" // uses default events defined in used scenario file
+    varlociraptor_fdr                 = 0.05  // default fdr for post-calling filtering
     varlociraptor_scenario_tumor_only = null  // uses default scenario in assets/varlociraptor_tumor_only.yte.yaml
     varlociraptor_scenario_somatic    = null  // uses default scenario in assets/varlociraptor_somatic.yte.yaml
     varlociraptor_scenario_germline   = null  // uses default scenario in assets/varlociraptor_germline.yte.yaml
diff --git a/nextflow_schema.json b/nextflow_schema.json
index ea943adb92..f50d769634 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -554,6 +554,26 @@
                     "description": "Number of chunks to split the vcf-files for varlociraptor. Minimum 1, indicates no splitting",
                     "hidden": true
                 },
+                "varlociraptor_events_tumor_only": {
+                    "type": "string",
+                    "default": "somatic_tumor_high somatic_tumor_low",
+                    "description": "Events to consider for FDR filtering for tumor only samples, must match events described in scenario file."
+                },
+                "varlociraptor_events_somatic": {
+                    "type": "string",
+                    "default": "somatic_tumor_high somatic_tumor_low",
+                    "description": "Events to consider for FDR filtering for somatic samples, must match events described in scenario file."
+                },
+                "varlociraptor_events_germline": {
+                    "type": "string",
+                    "default": "germline",
+                    "description": "Events to consider for FDR filtering for germline samples, must match events described in scenario file."
+                },
+                "varlociraptor_fdr": {
+                    "type": "number",
+                    "default": 0.05,
+                    "description": "The probability to be a false discovery may not exceed the given threshold."
+                },
                 "varlociraptor_scenario_tumor_only": {
                     "type": "string",
                     "description": "Yte compatible scenario file for tumor only samples. Defaults to assets/varlociraptor_tumor_only.yte.yaml"
diff --git a/subworkflows/local/post_variantcalling/main.nf b/subworkflows/local/post_variantcalling/main.nf
index 63e6a251d4..4afd0b576e 100644
--- a/subworkflows/local/post_variantcalling/main.nf
+++ b/subworkflows/local/post_variantcalling/main.nf
@@ -28,35 +28,39 @@ workflow POST_VARIANTCALLING {
     snv_consensus_calling
     normalize_vcfs
     varlociraptor_chunk_size // integer: [mandatory] [default: 15] number of chunks to split BCF files when preprocessing and calling variants
+    varlociraptor_events_germline
+    varlociraptor_events_somatic
+    varlociraptor_events_tumor_only
+    varlociraptor_fdr
     varlociraptor_scenario_germline
     varlociraptor_scenario_somatic
     varlociraptor_scenario_tumor_only
 
     main:
-    versions = Channel.empty()
-    vcfs = Channel.empty()
-    tbis = Channel.empty()
+    versions = channel.empty()
+    vcfs = channel.empty()
+    tbis = channel.empty()
 
     //
     // VARLOCIRAPTOR
     //
     if (tools && tools.split(',').contains('varlociraptor')) {
         // GERMLINE
-        VCF_VARLOCIRAPTOR_GERMLINE(cram_germline, fasta, fai, varlociraptor_scenario_germline, germline_vcfs, varlociraptor_chunk_size, 'normal')
+        VCF_VARLOCIRAPTOR_GERMLINE(cram_germline, fasta, fai, varlociraptor_scenario_germline, germline_vcfs, varlociraptor_chunk_size, 'normal', varlociraptor_events_germline, varlociraptor_fdr)
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.tbi)
         versions = versions.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.versions)
 
         // SOMATIC
-        VCF_VARLOCIRAPTOR_SOMATIC(cram_somatic, fasta, fai, varlociraptor_scenario_somatic, somatic_vcfs, germline_vcfs, varlociraptor_chunk_size)
+        VCF_VARLOCIRAPTOR_SOMATIC(cram_somatic, fasta, fai, varlociraptor_scenario_somatic, somatic_vcfs, germline_vcfs, varlociraptor_chunk_size, varlociraptor_events_somatic, varlociraptor_fdr)
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.tbi)
         versions = versions.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.versions)
 
         // TUMOR ONLY
-        VCF_VARLOCIRAPTOR_TUMOR_ONLY(cram_tumor_only, fasta, fai, varlociraptor_scenario_tumor_only, tumor_only_vcfs, varlociraptor_chunk_size, 'tumor')
+        VCF_VARLOCIRAPTOR_TUMOR_ONLY(cram_tumor_only, fasta, fai, varlociraptor_scenario_tumor_only, tumor_only_vcfs, varlociraptor_chunk_size, 'tumor', varlociraptor_events_tumor_only, varlociraptor_fdr)
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_TUMOR_ONLY.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_TUMOR_ONLY.out.tbi)
@@ -81,20 +85,20 @@ workflow POST_VARIANTCALLING {
 
         def excluded_variantcallers = ['manta', 'tiddit', 'samtools']
 
-        all_vcfs = Channel.empty().mix(germline_vcfs, tumor_only_vcfs, somatic_vcfs)
-                                .branch{ meta, vcf ->
+        all_vcfs = channel.empty().mix(germline_vcfs, tumor_only_vcfs, somatic_vcfs)
+                                .branch{ meta, _vcf ->
                                     small: small_variantcallers.contains(meta.variantcaller)
                                     other: true
                                 }
 
-        all_tbis = Channel.empty().mix(germline_tbis, tumor_only_tbis, somatic_tbis)
-                                .branch{ meta, tbi ->
+        all_tbis = channel.empty().mix(germline_tbis, tumor_only_tbis, somatic_tbis)
+                                .branch{ meta, _tbi ->
                                     small: small_variantcallers.contains(meta.variantcaller)
                                     other: true
                                 }
 
         // Validate that we're not silently excluding unknown variant callers
-        all_vcfs.other.subscribe { meta, vcf ->
+        all_vcfs.other.subscribe { meta, _vcf ->
             if (!excluded_variantcallers.contains(meta.variantcaller)) {
                 error("Variant caller '${meta.variantcaller}' is not in the small_variantcallers list and will be excluded from normalization/filtering/consensus. If this is a new SNV caller, please add it to the list in subworkflows/local/post_variantcalling/main.nf:78-80")
             }
diff --git a/subworkflows/local/vcf_varlociraptor_single/main.nf b/subworkflows/local/vcf_varlociraptor_single/main.nf
index 35d5efc228..2ba55d4af2 100644
--- a/subworkflows/local/vcf_varlociraptor_single/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_single/main.nf
@@ -4,6 +4,7 @@ include { BCFTOOLS_SORT as SORT_FINAL_VCF           } from '../../../modules/nf-
 include { RBT_VCFSPLIT                              } from '../../../modules/nf-core/rbt/vcfsplit'
 include { VARLOCIRAPTOR_CALLVARIANTS                } from '../../../modules/nf-core/varlociraptor/callvariants'
 include { VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES } from '../../../modules/nf-core/varlociraptor/estimatealignmentproperties'
+include { VARLOCIRAPTOR_FILTERFDR                   } from '../../../modules/nf-core/varlociraptor/filterfdr'
 include { VARLOCIRAPTOR_PREPROCESS                  } from '../../../modules/nf-core/varlociraptor/preprocess'
 include { YTE as FILL_SCENARIO_FILE                 } from '../../../modules/nf-core/yte'
 
@@ -16,6 +17,8 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
     ch_vcf
     val_num_chunks
     val_sampletype
+    val_events
+    val_fdr
 
     main:
     ch_versions = channel.empty()
@@ -132,7 +135,11 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
 
     ch_final_vcf = ch_sort_called_chunks_vcf.single.mix(CONCAT_CALLED_CHUNKS.out.vcf)
 
-    SORT_FINAL_VCF(ch_final_vcf)
+    VARLOCIRAPTOR_FILTERFDR(
+        ch_final_vcf.map { meta, vcf -> [ meta, vcf, val_events, val_fdr ] }
+    )
+
+    SORT_FINAL_VCF(VARLOCIRAPTOR_FILTERFDR.out.bcf)
 
     ch_versions = ch_versions.mix(SORT_FINAL_VCF.out.versions)
 
diff --git a/subworkflows/local/vcf_varlociraptor_somatic/main.nf b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
index dafba72787..4ad4c80cf5 100644
--- a/subworkflows/local/vcf_varlociraptor_somatic/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
@@ -9,6 +9,7 @@ include { RBT_VCFSPLIT
 include { VARLOCIRAPTOR_CALLVARIANTS                                              } from '../../../modules/nf-core/varlociraptor/callvariants'
 include { VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES as ALIGNMENTPROPERTIES_NORMAL } from '../../../modules/nf-core/varlociraptor/estimatealignmentproperties'
 include { VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES as ALIGNMENTPROPERTIES_TUMOR  } from '../../../modules/nf-core/varlociraptor/estimatealignmentproperties'
+include { VARLOCIRAPTOR_FILTERFDR                                                 } from '../../../modules/nf-core/varlociraptor/filterfdr'
 include { VARLOCIRAPTOR_PREPROCESS as PREPROCESS_NORMAL                           } from '../../../modules/nf-core/varlociraptor/preprocess'
 include { VARLOCIRAPTOR_PREPROCESS as PREPROCESS_TUMOR                            } from '../../../modules/nf-core/varlociraptor/preprocess'
 include { YTE as FILL_SCENARIO_FILE                                               } from '../../../modules/nf-core/yte'
@@ -22,6 +23,8 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     ch_somatic_vcf
     ch_germline_vcf
     val_num_chunks
+    val_events
+    val_fdr
 
     main:
     ch_versions = channel.empty()
@@ -283,7 +286,11 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
 
     ch_final_vcf = ch_sort_called_chunks_vcf.single.mix(CONCAT_CALLED_CHUNKS.out.vcf)
 
-    SORT_FINAL_VCF(ch_final_vcf)
+    VARLOCIRAPTOR_FILTERFDR(
+        ch_final_vcf.map { meta, vcf -> [ meta, vcf, val_events, val_fdr ] }
+    )
+
+    SORT_FINAL_VCF(VARLOCIRAPTOR_FILTERFDR.out.bcf)
 
     ch_versions = ch_versions.mix(SORT_FINAL_VCF.out.versions)
 
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index 53e38e3d29..c52c6f785f 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -1,13 +1,13 @@
 {
     "-profile test --tools strelka,varlociraptor --input recalibrated_somatic.csv": {
         "content": [
-            151,
+            153,
             {
                 "ALIGNMENTPROPERTIES_NORMAL": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "ALIGNMENTPROPERTIES_TUMOR": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "BCFTOOLS_STATS": {
                     "bcftools": 1.21
@@ -43,10 +43,10 @@
                     "mosdepth": "0.3.10"
                 },
                 "PREPROCESS_NORMAL": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "PREPROCESS_TUMOR": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "RBT_VCFSPLIT": {
                     "rbt": "0.42.2"
@@ -81,13 +81,16 @@
                     "tabix": 1.21
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
+                },
+                "VARLOCIRAPTOR_FILTERFDR": {
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_PREPROCESS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -305,8 +308,8 @@
                 "sample4_vs_sample3.strelka.merged.vcf.gz:md5,ecbb04bc2a4f802cac75f232d263ebe8"
             ],
             [
-                "sample3.strelka.germline.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=53245, phased=true, phasedAutodetect=false]",
-                "sample4_vs_sample3.strelka.somatic.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=2, variantCount=54634, phased=true, phasedAutodetect=false]"
+                "sample3.strelka.germline.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=65, phased=true, phasedAutodetect=false]",
+                "sample4_vs_sample3.strelka.somatic.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=2, variantCount=122, phased=true, phasedAutodetect=false]"
             ],
             "No warnings"
         ],
@@ -314,11 +317,11 @@
             "nf-test": "0.9.3",
             "nextflow": "25.10.4"
         },
-        "timestamp": "2025-11-02T16:48:49.54626"
+        "timestamp": "2026-06-12T14:56:17.705455093"
     },
     "-profile test --tools mutect2,varlociraptor --input recalibrated_tumoronly.csv": {
         "content": [
-            72,
+            73,
             {
                 "BCFTOOLS_STATS": {
                     "bcftools": 1.21
@@ -383,13 +386,16 @@
                     "tabix": "1.21"
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
+                },
+                "VARLOCIRAPTOR_FILTERFDR": {
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_PREPROCESS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -563,7 +569,7 @@
                 "sample2.mutect2.vcf.gz:md5,a53450657afc33f2a7b87fd75bf24267"
             ],
             [
-                "sample2.mutect2.tumor_only.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=2, variantCount=1258, phased=true, phasedAutodetect=false]"
+                "sample2.mutect2.tumor_only.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[], sampleCount=2, variantCount=0, phased=true, phasedAutodetect=true]"
             ],
             "No warnings"
         ],
@@ -571,11 +577,11 @@
             "nf-test": "0.9.3",
             "nextflow": "25.10.4"
         },
-        "timestamp": "2025-11-04T11:46:27.501146"
+        "timestamp": "2026-06-12T15:01:23.802458652"
     },
     "-profile test --tools strelka,varlociraptor --input recalibrated_germline.csv": {
         "content": [
-            67,
+            68,
             {
                 "BCFTOOLS_STATS": {
                     "bcftools": 1.21
@@ -628,13 +634,16 @@
                     "tabix": 1.21
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
+                },
+                "VARLOCIRAPTOR_FILTERFDR": {
+                    "varlociraptor": "8.9.5"
                 },
                 "VARLOCIRAPTOR_PREPROCESS": {
-                    "varlociraptor": "8.9.3"
+                    "varlociraptor": "8.9.5"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -796,7 +805,7 @@
                 "sample1.strelka.variants.vcf.gz:md5,5af34e7b632f604dc7a224f977fc2898"
             ],
             [
-                "sample1.strelka.germline.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=53245, phased=true, phasedAutodetect=false]"
+                "sample1.strelka.germline.varlociraptor.vcf.gz:summary,VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=65, phased=true, phasedAutodetect=false]"
             ],
             "No warnings"
         ],
@@ -804,6 +813,6 @@
             "nf-test": "0.9.3",
             "nextflow": "25.10.4"
         },
-        "timestamp": "2025-11-01T23:04:58.277878"
+        "timestamp": "2026-06-12T14:45:50.699233572"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index e8538b2abf..4962e9e856 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -527,7 +527,7 @@
                 "test.bcftools.bcftools_stats.txt:md5,4b2997064fb500f090639edca1ccaa6e",
                 "test.deepvariant.bcftools_stats.txt:md5,61ab450ed778088b9c879b8dcbbb9db8",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,dde124ceaf6f109cd274b837b950096b",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,6f942caec2f4f02c69e1216226fa44a9",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,189bc07d38c2a9ae34b141cff402bda6",
                 "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
                 "test2_vs_test.strelka.somatic_indels.bcftools_stats.txt:md5,5e8f9a8fdbc765ced736d0c8c7dd3a52",
                 "test2_vs_test.strelka.somatic_snvs.bcftools_stats.txt:md5,edb7763fad7b6f825e47e01ffa70adbc",
@@ -559,7 +559,7 @@
                 "test.deepvariant.TsTv.count:md5,d3c138521fd95eb45f6e495f39d47404",
                 "test.freebayes.filtered.FILTER.summary:md5,87e753ba2ad969475fb55661852f75e0",
                 "test.freebayes.filtered.TsTv.count:md5,845f64e5bb4224af98f3a47294cd5483",
-                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,126e83dcd37b82420f7c5d7b235479f1",
+                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,1f06f857e3576c6a98c32d1e7f51f456",
                 "test2_vs_test.freebayes.filtered.TsTv.count:md5,28919c7d29c998681391d2027af3e0f9",
                 "test.strelka.variants.FILTER.summary:md5,dd87f507da7de20d5318841af312493b",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
@@ -612,7 +612,7 @@
                 ],
                 [
                     "test2_vs_test.freebayes.vcf.gz",
-                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=1447, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=1453, phased=false, phasedAutodetect=false]"
                 ]
             ],
             [
@@ -637,9 +637,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2026-01-08T16:27:17.419740053"
+        "timestamp": "2026-06-12T16:20:15.694178204"
     },
     "-profile test --input tests/csv/3.0/fastq_single.csv --tools cnvkit,deepvariant,freebayes,mpileup,strelka,tiddit": {
         "content": [
@@ -1132,9 +1132,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2026-01-08T12:49:19.475669979"
+        "timestamp": "2026-06-12T16:23:41.706393706"
     },
     "-profile test --input tests/csv/3.0/fastq_tumor_only.csv --tools cnvkit,freebayes,mpileup,mutect2,tiddit": {
         "content": [
@@ -1590,8 +1590,8 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2026-01-08T14:32:16.644778218"
+        "timestamp": "2026-06-12T16:16:13.675589663"
     }
-}
+}
\ No newline at end of file
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 63fb88199e..79e58083b4 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -555,7 +555,7 @@
                 "samtools_alignment_plot.txt:md5,8e6178a26fe2a4fc4f45fac3175ba6c6",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,dde124ceaf6f109cd274b837b950096b",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,6f942caec2f4f02c69e1216226fa44a9",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,189bc07d38c2a9ae34b141cff402bda6",
                 "test.md.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.md.mosdepth.summary.txt:md5,0010c2396a3173c7cf4983abe2eb6a4c",
                 "test.md.per-base.bed.gz:md5,34dfe443c0a0767562dd65272e3310ef",
@@ -574,7 +574,7 @@
                 "test2.recal.per-base.bed.gz.csi:md5,4205a09ede17cdbdaad45e3553f73105",
                 "test.freebayes.filtered.FILTER.summary:md5,87e753ba2ad969475fb55661852f75e0",
                 "test.freebayes.filtered.TsTv.count:md5,845f64e5bb4224af98f3a47294cd5483",
-                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,126e83dcd37b82420f7c5d7b235479f1",
+                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,1f06f857e3576c6a98c32d1e7f51f456",
                 "test2_vs_test.freebayes.filtered.TsTv.count:md5,28919c7d29c998681391d2027af3e0f9"
             ],
             "No BAM files",
@@ -591,7 +591,7 @@
                 ],
                 [
                     "test2_vs_test.freebayes.vcf.gz",
-                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=1447, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=1453, phased=false, phasedAutodetect=false]"
                 ]
             ],
             [
@@ -601,7 +601,7 @@
                 ],
                 [
                     "test2_vs_test.freebayes.filtered.vcf.gz",
-                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=137, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=125, phased=false, phasedAutodetect=false]"
                 ]
             ],
             [
@@ -611,9 +611,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-12-15T22:43:02.382544129"
+        "timestamp": "2026-06-12T11:36:14.240620697"
     },
     "-profile test --tools freebayes --wes --nucleotides_per_second 20": {
         "content": [
@@ -980,9 +980,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-12-15T22:35:44.029764132"
+        "timestamp": "2026-06-12T11:27:28.609439333"
     },
     "-profile test --tools freebayes --no_intervals": {
         "content": [
@@ -1331,9 +1331,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-12-15T22:38:03.564960868"
+        "timestamp": "2026-06-12T11:30:11.341607202"
     },
     "-profile test --tools freebayes --no_intervals --input recalibrated_tumoronly.csv": {
         "content": [
@@ -1903,7 +1903,7 @@
                 "samtools_alignment_plot.txt:md5,89d0a6e7076223e9feadbecd794948d5",
                 "samtools_insert_size.txt:md5,ca54b785b1d63edd61cef58cd5558aa2",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,b313075b3e9854e0b561dd8d754bf097",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,17df64b64f9235afa93aae6bbe08e283",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,42d0644fc84df43b26e8de1ad427b446",
                 "test.md.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.md.mosdepth.region.dist.txt:md5,835fdc6fa52cc33e6fb76c0c20a8a6c3",
                 "test.md.mosdepth.summary.txt:md5,dcc9ab2bf3248903e02d8da87e678977",
@@ -1934,7 +1934,7 @@
                 "test2.recal.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
                 "test.freebayes.filtered.FILTER.summary:md5,449597c35ada505b4cb2530d5260e9d5",
                 "test.freebayes.filtered.TsTv.count:md5,162253eb6c406300678985b3ac7dc868",
-                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,0ae7467f2311c1382173d70d8d7efb0b",
+                "test2_vs_test.freebayes.filtered.FILTER.summary:md5,b3cdcab0fb3a666b40ef76c4193ff7f8",
                 "test2_vs_test.freebayes.filtered.TsTv.count:md5,9dc940f98dae9c0b49c9468a491836d4"
             ],
             "No BAM files",
@@ -1951,12 +1951,12 @@
                 ],
                 [
                     "test2_vs_test.freebayes.vcf.gz",
-                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=966, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr22], sampleCount=2, variantCount=988, phased=false, phasedAutodetect=false]"
                 ]
             ],
             [
                 "test.freebayes.filtered.vcf.gz:md5,c4793897a38d6781dc512a52d9046be5",
-                "test2_vs_test.freebayes.filtered.vcf.gz:md5,d1d0916fc56a666bd7637792047b82f8"
+                "test2_vs_test.freebayes.filtered.vcf.gz:md5,6da8e1304355fedec62419d5ae1a402c"
             ],
             [
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1",
@@ -1965,8 +1965,8 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-12-15T22:40:36.195304962"
+        "timestamp": "2026-06-12T11:33:20.327931788"
     }
-}
+}
\ No newline at end of file
diff --git a/workflows/sarek/main.nf b/workflows/sarek/main.nf
index 29bffe1f24..06bb7d9ff8 100644
--- a/workflows/sarek/main.nf
+++ b/workflows/sarek/main.nf
@@ -107,6 +107,11 @@ workflow SAREK {
     pon
     pon_tbi
     sentieon_dnascope_model
+    varlociraptor_chunk_size
+    varlociraptor_events_germline
+    varlociraptor_events_somatic
+    varlociraptor_events_tumor_only
+    varlociraptor_fdr
     varlociraptor_scenario_germline
     varlociraptor_scenario_somatic
     varlociraptor_scenario_tumor_only
@@ -531,7 +536,11 @@ workflow SAREK {
             params.filter_vcfs,
             params.snv_consensus_calling,
             params.normalize_vcfs,
-            params.varlociraptor_chunk_size,
+            varlociraptor_chunk_size,
+            varlociraptor_events_germline,
+            varlociraptor_events_somatic,
+            varlociraptor_events_tumor_only,
+            varlociraptor_fdr,
             varlociraptor_scenario_germline,
             varlociraptor_scenario_somatic,
             varlociraptor_scenario_tumor_only,

From bd02bf289d768167a399807fcfc15e5e2582f4b9 Mon Sep 17 00:00:00 2001
From: Maxime U Garcia <max.u.garcia@gmail.com>
Date: Wed, 8 Jul 2026 15:25:38 +0200
Subject: [PATCH 03/27] Update EnsemblVEP to 116.0 (#2229)

---
 CHANGELOG.md                                  |  9 +++++
 conf/igenomes.config                          |  6 +--
 docs/DEVELOPER_GUIDELINES.md                  |  6 +++
 modules.json                                  |  4 +-
 .../ensemblvep/download/environment.yml       |  2 +-
 modules/nf-core/ensemblvep/download/main.nf   |  4 +-
 .../nf-core/ensemblvep/vep/environment.yml    |  2 +-
 modules/nf-core/ensemblvep/vep/main.nf        |  4 +-
 nextflow.config                               |  2 +-
 nextflow_schema.json                          |  2 +-
 nf-test.config                                |  2 +-
 tests/annotation_merge.nf.test.snap           | 20 +++++-----
 tests/annotation_vep.nf.test                  |  1 +
 tests/annotation_vep.nf.test.snap             |  9 +++--
 tests/lib/UTILS.groovy                        | 40 +++++++++++--------
 tests/samplesheets.nf.test.snap               |  3 +-
 16 files changed, 71 insertions(+), 45 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 37b5bdc04d..517dfd8eab 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
+- [#2229](https://github.com/nf-core/sarek/pull/2229) - Update EnsemblVEP to 116.0
+
 ### Fixed
 
 ### Removed
@@ -22,6 +24,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | Dependency    | Old version | New version |
 | ------------- | ----------- | ----------- |
 | varlociraptor | 8.9.3       | 8.9.5       |
+| ensembl-vep   | 115.2       | 116.0       |
 
 ### Dependencies - plugins
 
@@ -37,6 +40,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | `--varlociraptor_events_tumor_only` | New    |
 | `--varlociraptor_fdr`               | New    |
 
+| Parameter   | Old default | New default |
+| ----------- | ----------- | ----------- |
+| vep_version | 115.2-1     | 116.0-0     |
+
 ### Developer section
 
 #### Added
@@ -46,10 +53,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 #### Changed
 
 - [#2225](https://github.com/nf-core/sarek/pull/2225) - Back to dev (3.9.1dev)
+- [#2229](https://github.com/nf-core/sarek/pull/2229) - Update nft-utils to 1.0.0, migrate `getAllFilesFromDir` to `getAllFilesFromPath` in test utilities
 
 #### Fixed
 
 - [#2208](https://github.com/nf-core/sarek/pull/2208) - Update freebayes params (remove `--pooled-discrete` and change `--min-alternate-fraction` from 0.03 to 0.01), move chunk_size param
+- [#2229](https://github.com/nf-core/sarek/pull/2229) - Fix LoFTEE test to validate CSQ fields instead of asserting nothing
 
 #### Removed
 
diff --git a/conf/igenomes.config b/conf/igenomes.config
index b665518af5..e47fef8c2e 100644
--- a/conf/igenomes.config
+++ b/conf/igenomes.config
@@ -77,7 +77,7 @@ params {
             pon_tbi                 = "${params.igenomes_base}/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/1000g_pon.hg38.vcf.gz.tbi"
             sentieon_dnascope_model = "${params.igenomes_base}/Homo_sapiens/GATK/GRCh38/Annotation/Sentieon/SentieonDNAscopeModel1.1.model"
             snpeff_db               = 'GRCh38.99'
-            vep_cache_version       = '115'
+            vep_cache_version       = '116'
             vep_genome              = 'GRCh38'
             vep_species             = 'homo_sapiens'
         }
@@ -96,7 +96,7 @@ params {
             fasta                 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
             ngscheckmate_bed      ="${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/NGSCheckMate/SNP_GRCh38_hg38_wChr.bed"
             snpeff_db             = 'GRCh38.99'
-            vep_cache_version     = '115'
+            vep_cache_version     = '116'
             vep_genome            = 'GRCh38'
             vep_species           = 'homo_sapiens'
         }
@@ -239,7 +239,7 @@ params {
             bwa                   = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/"
             fasta                 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
             snpeff_db             = 'GRCh38.99'
-            vep_cache_version     = '115'
+            vep_cache_version     = '116'
             vep_genome            = 'GRCh38'
             vep_species           = 'homo_sapiens'
         }
diff --git a/docs/DEVELOPER_GUIDELINES.md b/docs/DEVELOPER_GUIDELINES.md
index eac5205ee6..9710248b23 100644
--- a/docs/DEVELOPER_GUIDELINES.md
+++ b/docs/DEVELOPER_GUIDELINES.md
@@ -489,6 +489,12 @@ nf-core modules update <tool>/<subcommand>
 nf-core modules list local
 ```
 
+### Updating VEP modules
+
+When updating `ensemblvep/vep` module, always update the `vep_version` parameter to match the new VEP version. This parameter is used by the LoFTEE plugin to locate the VEP installation path (e.g. `/opt/conda/share/ensembl-vep-${vep_version}`).
+
+Also update `vep_cache_version` in `conf/igenomes.config` for available genomes, based on what's available on [annotation-cache](https://annotation-cache.github.io/ensemblvep/). Not all genomes may have a cache for the new version — only update those that do.
+
 ---
 
 ## Subworkflows
diff --git a/modules.json b/modules.json
index ca3721a53e..c77387364e 100644
--- a/modules.json
+++ b/modules.json
@@ -163,12 +163,12 @@
                     },
                     "ensemblvep/download": {
                         "branch": "master",
-                        "git_sha": "251885fdb29eca03523d326aca5c827d1f6bdfeb",
+                        "git_sha": "db055f5f8c726a924bf51dadce3978da3bbdad7e",
                         "installed_by": ["cache_download_ensemblvep_snpeff", "modules"]
                     },
                     "ensemblvep/vep": {
                         "branch": "master",
-                        "git_sha": "251885fdb29eca03523d326aca5c827d1f6bdfeb",
+                        "git_sha": "db055f5f8c726a924bf51dadce3978da3bbdad7e",
                         "installed_by": ["modules"]
                     },
                     "fastp": {
diff --git a/modules/nf-core/ensemblvep/download/environment.yml b/modules/nf-core/ensemblvep/download/environment.yml
index 48fc515c72..3d904beca0 100644
--- a/modules/nf-core/ensemblvep/download/environment.yml
+++ b/modules/nf-core/ensemblvep/download/environment.yml
@@ -5,7 +5,7 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/ensembl-vep
-  - bioconda::ensembl-vep=115.2
+  - bioconda::ensembl-vep=116.0
   # renovate: datasource=conda depName=bioconda/perl-math-cdf
   - bioconda::perl-math-cdf=0.1
   # renovate: datasource=conda depName=bioconda/htslib
diff --git a/modules/nf-core/ensemblvep/download/main.nf b/modules/nf-core/ensemblvep/download/main.nf
index 199acfdf32..1f7c19a3f3 100644
--- a/modules/nf-core/ensemblvep/download/main.nf
+++ b/modules/nf-core/ensemblvep/download/main.nf
@@ -4,8 +4,8 @@ process ENSEMBLVEP_DOWNLOAD {
 
     conda "${moduleDir}/environment.yml"
     container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ed/edd02dfaf968d06c808e3c208d5b3e86afb4259590bfa6e5499965ef3bc81881/data'
-        : 'community.wave.seqera.io/library/ensembl-vep_perl-math-cdf_htslib:efd9a6d1c5f218a9'}"
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/11/112b7b57f93b053ccd3f8b2f2207a5faa629fd4ea181af8e1a41a1fbd007e657/data'
+        : 'community.wave.seqera.io/library/ensembl-vep_perl-math-cdf_htslib:c4edd3fb4a233ae6'}"
 
     input:
     tuple val(meta), val(assembly), val(species), val(cache_version)
diff --git a/modules/nf-core/ensemblvep/vep/environment.yml b/modules/nf-core/ensemblvep/vep/environment.yml
index 48fc515c72..3d904beca0 100644
--- a/modules/nf-core/ensemblvep/vep/environment.yml
+++ b/modules/nf-core/ensemblvep/vep/environment.yml
@@ -5,7 +5,7 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/ensembl-vep
-  - bioconda::ensembl-vep=115.2
+  - bioconda::ensembl-vep=116.0
   # renovate: datasource=conda depName=bioconda/perl-math-cdf
   - bioconda::perl-math-cdf=0.1
   # renovate: datasource=conda depName=bioconda/htslib
diff --git a/modules/nf-core/ensemblvep/vep/main.nf b/modules/nf-core/ensemblvep/vep/main.nf
index 4433eda936..914bc92932 100644
--- a/modules/nf-core/ensemblvep/vep/main.nf
+++ b/modules/nf-core/ensemblvep/vep/main.nf
@@ -4,8 +4,8 @@ process ENSEMBLVEP_VEP {
 
     conda "${moduleDir}/environment.yml"
     container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ed/edd02dfaf968d06c808e3c208d5b3e86afb4259590bfa6e5499965ef3bc81881/data'
-        : 'community.wave.seqera.io/library/ensembl-vep_perl-math-cdf_htslib:efd9a6d1c5f218a9'}"
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/11/112b7b57f93b053ccd3f8b2f2207a5faa629fd4ea181af8e1a41a1fbd007e657/data'
+        : 'community.wave.seqera.io/library/ensembl-vep_perl-math-cdf_htslib:c4edd3fb4a233ae6'}"
 
     input:
     tuple val(meta), path(vcf), path(custom_extra_files)
diff --git a/nextflow.config b/nextflow.config
index d8e204796f..92652de02d 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -142,7 +142,7 @@ params {
     vep_cache_preflight_check = false // No preflight check by default
     vep_spliceai              = null // spliceai plugin disabled within VEP
     vep_spliceregion          = null // spliceregion plugin disabled within VEP
-    vep_version               = "115.2-1"      // Should be updated when we update VEP, needs this to get full path to some plugins
+    vep_version               = "116.0-0"      // Should be updated when we update VEP, needs this to get full path to some plugins
 
     // MultiQC options
     multiqc_config              = null
diff --git a/nextflow_schema.json b/nextflow_schema.json
index f50d769634..19ef1af732 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -777,7 +777,7 @@
                 },
                 "vep_version": {
                     "type": "string",
-                    "default": "115.2-1",
+                    "default": "116.0-0",
                     "fa_icon": "fas fa-toolbox",
                     "description": "Should reflect the VEP version used in the container.",
                     "help_text": "Used by the loftee plugin which needs the full path to the VEP installation (e.g. `/opt/conda/share/ensembl-vep-${vep_version}`). Update this when the VEP container version changes."
diff --git a/nf-test.config b/nf-test.config
index 0676bf9351..c9c6b63ae2 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -20,7 +20,7 @@ config {
     // load the necessary plugins
     plugins {
         load "nft-bam@0.6.1"
-        load "nft-utils@0.0.9"
+        load "nft-utils@1.0.0"
         load "nft-vcf@1.0.7"
     }
 }
diff --git a/tests/annotation_merge.nf.test.snap b/tests/annotation_merge.nf.test.snap
index d0302c0e3a..1c23b195ce 100644
--- a/tests/annotation_merge.nf.test.snap
+++ b/tests/annotation_merge.nf.test.snap
@@ -4,7 +4,7 @@
             5,
             {
                 "ENSEMBLVEP_VEP": {
-                    "ensemblvep": "115.2",
+                    "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 },
@@ -16,7 +16,7 @@
                     "tabix": "1.21"
                 },
                 "VCF_ANNOTATE_MERGE": {
-                    "ensemblvep": "115.2",
+                    "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 }
@@ -109,10 +109,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-27T10:13:01.036308477",
+        "timestamp": "2026-07-08T10:14:04.788318141",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "26.04.4"
         }
     },
     "-profile test --tools merge,snpsift": {
@@ -133,7 +133,7 @@
                     "tabix": "1.21"
                 },
                 "VCF_ANNOTATE_MERGE": {
-                    "ensemblvep": "115.2",
+                    "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 }
@@ -220,10 +220,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-27T10:14:01.977520509",
+        "timestamp": "2026-07-08T10:15:44.567611717",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "26.04.4"
         }
     },
     "-profile test --tools merge": {
@@ -238,7 +238,7 @@
                     "tabix": "1.21"
                 },
                 "VCF_ANNOTATE_MERGE": {
-                    "ensemblvep": "115.2",
+                    "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 }
@@ -320,10 +320,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-27T10:12:05.371914045",
+        "timestamp": "2026-07-08T10:12:53.660022078",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "26.04.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/annotation_vep.nf.test b/tests/annotation_vep.nf.test
index 82adf86763..0f7c12add7 100644
--- a/tests/annotation_vep.nf.test
+++ b/tests/annotation_vep.nf.test
@@ -41,6 +41,7 @@ nextflow_pipeline {
                 tools: 'vep',
                 vep_loftee: true,
             ],
+            vcf_header_check: '|LoF|',
         ],
     ]
 
diff --git a/tests/annotation_vep.nf.test.snap b/tests/annotation_vep.nf.test.snap
index e77e69ae4d..4365a3d7a3 100644
--- a/tests/annotation_vep.nf.test.snap
+++ b/tests/annotation_vep.nf.test.snap
@@ -4,7 +4,7 @@
             2,
             {
                 "ENSEMBLVEP_VEP": {
-                    "ensemblvep": "115.2",
+                    "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 }
@@ -52,12 +52,15 @@
             [
                 "test_VEP.ann.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
             ],
+            [
+                "test_VEP.ann.vcf.gz:header.contains(|LoF|),true"
+            ],
             "No warnings"
         ],
-        "timestamp": "2026-05-27T13:40:41.25343459",
+        "timestamp": "2026-07-08T10:26:06.53157943",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "26.04.4"
         }
     },
     "Fails with profile test --dbnsfp and no dbnsfp_tbi": {
diff --git a/tests/lib/UTILS.groovy b/tests/lib/UTILS.groovy
index faa76e26ef..445c1c092f 100644
--- a/tests/lib/UTILS.groovy
+++ b/tests/lib/UTILS.groovy
@@ -14,6 +14,7 @@ class UTILS {
 
         // These strings are not stable and should be ignored
         def snapshot_ignore_list = [
+            "Check script",
             "Creating env using",
             "Downloading plugin",
             "Got an interrupted  exception while taking agent result",
@@ -24,26 +25,28 @@ class UTILS {
         ]
 
         // stable_name: All files + folders in ${outdir}/ with a stable name
-        def stable_name = getAllFilesFromDir(outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
+        def stable_name = getAllFilesFromPath(outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}'])
         // stable_content: All files in ${outdir}/ with stable content
-        def stable_content = getAllFilesFromDir(outdir, ignoreFile: 'tests/.nftignore', ignore: [scenario.ignoreFiles ])
+        def stable_content = getAllFilesFromPath(outdir, ignoreFile: 'tests/.nftignore', ignore: [scenario.ignoreFiles])
         // bam_files: All bam files
-        def bam_files = getAllFilesFromDir(outdir, include: ['**/*.bam'], ignore: [scenario.ignoreFiles ])
+        def bam_files = getAllFilesFromPath(outdir, include: ['**/*.bam'], ignore: [scenario.ignoreFiles])
         // cram_files: All cram files
-        def cram_files = getAllFilesFromDir(outdir, include: ['**/*.cram'], ignore: [scenario.ignoreFiles ])
+        def cram_files = getAllFilesFromPath(outdir, include: ['**/*.cram'], ignore: [scenario.ignoreFiles])
         // Fasta file for cram verification with nft-bam
         def fasta_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
         def fasta = fasta_base + 'genomics/homo_sapiens/genome/genome.fasta'
         // txt_files: MuSE txt files
-        def txt_files = getAllFilesFromDir(outdir, include: ['**/*.MuSE.txt'])
+        def txt_files = getAllFilesFromPath(outdir, include: ['**/*.MuSE.txt'])
         // vcf_files: All vcf files
-        def vcf_files = getAllFilesFromDir(outdir, include: ['**/*.vcf{,.gz}'], ignore: [scenario.ignoreFiles ])
+        def vcf_files = getAllFilesFromPath(outdir, include: ['**/*.vcf{,.gz}'], ignore: [scenario.ignoreFiles])
         // freebayes_unfiltered: vcf files from freebayes without quality filtering
-        def freebayes_unfiltered = getAllFilesFromDir(outdir, include: ['**/*.freebayes.vcf.gz'])
+        def freebayes_unfiltered = getAllFilesFromPath(outdir, include: ['**/*.freebayes.vcf.gz'])
         // varlociraptor vcf
-        def varlociraptor_vcf = getAllFilesFromDir(outdir, include: ['**/*.varlociraptor.{vcf}{,.gz}'])
+        def varlociraptor_vcf = getAllFilesFromPath(outdir, include: ['**/*.varlociraptor.{vcf}{,.gz}'])
 
         def assertion = []
+        // getAllFilesFromPath returns relative paths (strings), so this resolves to an absolute path
+        def absolutePath = { file -> file.toString().startsWith('/') ? file.toString() : "${outdir}/${file}" }
 
         if (!scenario.failure) {
             assertion.add(workflow.trace.succeeded().size())
@@ -54,27 +57,32 @@ class UTILS {
         assertion.add(stable_name)
 
         if (!scenario.stub) {
-            assertion.add(stable_content.isEmpty() ? 'No stable content' : stable_content)
-            assertion.add(bam_files.isEmpty() ? 'No BAM files' : bam_files.collect { file -> file.getName() + ":md5," + bam(file.toString()).readsMD5 })
-            assertion.add(cram_files.isEmpty() ? 'No CRAM files' : cram_files.collect { file -> file.getName() + ":md5," + cram(file.toString(), fasta).readsMD5 })
+            assertion.add(stable_content.isEmpty() ? 'No stable content' : stable_content.collect { file -> path(absolutePath(file)) })
+            assertion.add(bam_files.isEmpty() ? 'No BAM files' : bam_files.collect { file -> file.tokenize('/').last() + ":md5," + bam(absolutePath(file)).readsMD5 })
+            assertion.add(cram_files.isEmpty() ? 'No CRAM files' : cram_files.collect { file -> file.tokenize('/').last() + ":md5," + cram(absolutePath(file), fasta).readsMD5 })
             if (scenario.include_muse_txt) {
                 // It will skip the first line of the txt file
-                assertion.add(txt_files.isEmpty() ? 'No TXT files' : txt_files.collect{ file -> file.getName() + ":md5," + file.readLines()[2..-1].join('\n').md5() })
+                assertion.add(txt_files.isEmpty() ? 'No TXT files' : txt_files.collect { file -> file.tokenize('/').last() + ":md5," + path(absolutePath(file)).readLines().drop(2).join('\n').md5() })
             }
             if (scenario.include_freebayes_unfiltered) {
                 // It will only print the vcf summary to avoid differing md5sums because of small differences in QUAL score
-                assertion.add(freebayes_unfiltered.isEmpty() ? 'No Freebayes unfiltered VCF files' : freebayes_unfiltered.collect { file -> [ file.getName(), path(file.toString()).vcf.summary ] })
+                assertion.add(freebayes_unfiltered.isEmpty() ? 'No Freebayes unfiltered VCF files' : freebayes_unfiltered.collect { file -> [ file.tokenize('/').last(), path(absolutePath(file)).vcf.summary ] })
             }
             if (scenario.no_vcf_md5sum) {
                 // Will print the summary instead of the md5sum for vcf files
-                assertion.add(vcf_files.isEmpty() ? 'No VCF files' : vcf_files.collect { file -> [ file.getName(), path(file.toString()).vcf.summary ] })
+                assertion.add(vcf_files.isEmpty() ? 'No VCF files' : vcf_files.collect { file -> [ file.tokenize('/').last(), path(absolutePath(file)).vcf.summary ] })
             } else {
-                assertion.add(vcf_files.isEmpty() ? 'No VCF files' : vcf_files.collect { file -> file.getName() + ":md5," + path(file.toString()).vcf.variantsMD5 })
+                assertion.add(vcf_files.isEmpty() ? 'No VCF files' : vcf_files.collect { file -> file.tokenize('/').last() + ":md5," + path(absolutePath(file)).vcf.variantsMD5 })
                 if (scenario.include_varlociraptor_vcf) {
                     // It will use the summary method to extract the vcf file content
-                    assertion.add(varlociraptor_vcf.isEmpty() ? 'No Varlociraptor VCF files' : varlociraptor_vcf.collect { file -> file.getName() + ":summary," + path(file.toString()).vcf.summary })
+                    assertion.add(varlociraptor_vcf.isEmpty() ? 'No Varlociraptor VCF files' : varlociraptor_vcf.collect { file -> file.tokenize('/').last() + ":summary," + path(absolutePath(file)).vcf.summary })
                 }
             }
+
+            // Check for specific VCF header fields if requested
+            if (scenario.vcf_header_check) {
+                assertion.add(vcf_files.isEmpty() ? 'No VCF files' : vcf_files.collect { file -> file.tokenize('/').last() + ":header.contains(${scenario.vcf_header_check})," + path(absolutePath(file)).vcf.header.toString().contains(scenario.vcf_header_check) })
+            }
         }
 
         // If we have a snapshot options in scenario then we allow to capture either stderr, stdout or both
diff --git a/tests/samplesheets.nf.test.snap b/tests/samplesheets.nf.test.snap
index 3f2ff5acb7..a36cd61047 100644
--- a/tests/samplesheets.nf.test.snap
+++ b/tests/samplesheets.nf.test.snap
@@ -29,8 +29,7 @@
             [
                 "The following invalid input values have been detected:",
                 "* --input ([PATH]/./tests/csv/3.0/fastq_sample_with_space.csv): Validation of file failed:",
-                "\t-> Entry 2: Error for field 'sample' (test 2): \"test 2\" does not match regular expression [^\\S+$] (Sample ID must be provided, cannot contain spaces and must be a string value)",
-                " -- Check script '[PATH]/subworkflows/nf-core/utils_nfschema_plugin/main.nf' at line: 72 or see '[PATH]/tests/[NFT_HASH]/meta/nextflow.log' file for more details"
+                "\t-> Entry 2: Error for field 'sample' (test 2): \"test 2\" does not match regular expression [^\\S+$] (Sample ID must be provided, cannot contain spaces and must be a string value)"
             ]
         ],
         "meta": {

From 2d44030d286f2308eae5d5af85eef738e3bb5e1e Mon Sep 17 00:00:00 2001
From: nf-core bot <core@nf-co.re>
Date: Mon, 13 Jul 2026 15:27:57 +0200
Subject: [PATCH 04/27] Important! Template update for nf-core/tools v4.0.2
 (#2178)

Co-authored-by: Maxime U. Garcia <max.u.garcia@gmail.com>
---
 .devcontainer/devcontainer.json               |   1 +
 .github/CONTRIBUTING.md                       | 128 ---
 .github/PULL_REQUEST_TEMPLATE.md              |   4 +-
 .github/actions/get-shards/action.yml         |   2 +-
 .../{cloudtest.yml => awsfulltest.yml}        |  16 +-
 .github/workflows/awstest.yml                 |  33 +
 .github/workflows/branch.yml                  |   2 +-
 .github/workflows/clean-up.yml                |   2 +-
 .github/workflows/download_pipeline.yml       |  16 +-
 .github/workflows/fix_linting.yml             |  22 +-
 .github/workflows/linting.yml                 |  34 +-
 .github/workflows/linting_comment.yml         |   4 +-
 .github/workflows/nf-test-gpu.yml             |  11 +-
 .github/workflows/nf-test-sentieon.yml        |  11 +-
 .github/workflows/nf-test.yml                 |  19 +-
 .github/workflows/release-announcements.yml   |   4 +-
 .../workflows/template-version-comment.yml    |   6 +-
 .nf-core.yml                                  |   6 +-
 CHANGELOG.md                                  |   1 +
 README.md                                     |   6 +-
 conf/containers_conda_lock_files_amd64.config |   3 +-
 conf/containers_conda_lock_files_arm64.config |   3 +-
 conf/containers_docker_amd64.config           |   3 +-
 conf/containers_docker_arm64.config           |   3 +-
 .../containers_singularity_https_amd64.config |   3 +-
 .../containers_singularity_https_arm64.config |   3 +-
 conf/containers_singularity_oras_amd64.config |   3 +-
 conf/containers_singularity_oras_arm64.config |   3 +-
 docs/CONTRIBUTING.md                          | 201 +++++
 modules.json                                  |   4 +-
 .../linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt     | 822 ++++++++++++++++++
 .../linux_arm64-bd-e455e32f745abe68_1.txt     | 769 ++++++++++++++++
 modules/nf-core/fastqc/main.nf                |  43 +-
 modules/nf-core/fastqc/meta.yml               |  51 +-
 ... => linux_amd64-bd-c17fb751507e9dfc_1.txt} | 225 ++---
 ... => linux_arm64-bd-5c84a5000a226ab5_1.txt} | 226 ++---
 modules/nf-core/multiqc/main.nf               |   4 +-
 modules/nf-core/multiqc/meta.yml              |  28 +-
 nextflow.config                               |   2 +-
 nf-test.config                                |  26 +-
 ro-crate-metadata.json                        | 112 +--
 tests/samplesheets.nf.test                    |   5 -
 tests/samplesheets.nf.test.snap               |  59 +-
 tests/variant_calling_muse.nf.test            |   2 +-
 workflows/{sarek/main.nf => sarek.nf}         |  39 +-
 45 files changed, 2381 insertions(+), 589 deletions(-)
 delete mode 100644 .github/CONTRIBUTING.md
 rename .github/workflows/{cloudtest.yml => awsfulltest.yml} (91%)
 create mode 100644 .github/workflows/awstest.yml
 create mode 100644 docs/CONTRIBUTING.md
 create mode 100644 modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt
 create mode 100644 modules/nf-core/fastqc/.conda-lock/linux_arm64-bd-e455e32f745abe68_1.txt
 rename modules/nf-core/multiqc/.conda-lock/{linux_amd64-bd-839587b417d23042_1.txt => linux_amd64-bd-c17fb751507e9dfc_1.txt} (92%)
 rename modules/nf-core/multiqc/.conda-lock/{linux_arm64-bd-3e45d17b40a576b4_1.txt => linux_arm64-bd-5c84a5000a226ab5_1.txt} (92%)
 rename workflows/{sarek/main.nf => sarek.nf} (96%)

diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json
index 97c8c97fe3..237c9ed083 100644
--- a/.devcontainer/devcontainer.json
+++ b/.devcontainer/devcontainer.json
@@ -1,4 +1,5 @@
 {
+    "$schema": "https://raw.githubusercontent.com/devcontainers/spec/main/schemas/devContainer.schema.json",
     "name": "nfcore",
     "image": "nfcore/devcontainer:latest",
 
diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md
deleted file mode 100644
index 6e9b1635e6..0000000000
--- a/.github/CONTRIBUTING.md
+++ /dev/null
@@ -1,128 +0,0 @@
-# `nf-core/sarek`: Contributing Guidelines
-
-Hi there!
-Many thanks for taking an interest in improving nf-core/sarek.
-
-We try to manage the required tasks for nf-core/sarek using GitHub issues, you probably came to this page when creating one.
-Please use the pre-filled template to save time.
-
-However, don't be put off by this template - other more general issues and suggestions are welcome!
-Contributions to the code are even more welcome ;)
-
-> [!NOTE]
-> If you need help using or modifying nf-core/sarek then the best place to ask is on the nf-core Slack [#sarek](https://nfcore.slack.com/channels/sarek) channel ([join our Slack here](https://nf-co.re/join/slack)).
-
-## Contribution workflow
-
-If you'd like to write some code for nf-core/sarek, the standard workflow is as follows:
-
-1. Check that there isn't already an issue about your idea in the [nf-core/sarek issues](https://github.com/nf-core/sarek/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this
-2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/sarek repository](https://github.com/nf-core/sarek) to your GitHub account
-3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions)
-4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10).
-5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged
-
-If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/).
-
-## Tests
-
-You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command:
-
-```bash
-nf-test test --profile debug,test,docker --verbose
-```
-
-When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests.
-Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then.
-
-There are typically two types of tests that run:
-
-### Lint tests
-
-`nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to.
-To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint <pipeline-directory>` command.
-
-If any failures or warnings are encountered, please follow the listed URL for more documentation.
-
-### Pipeline tests
-
-Each `nf-core` pipeline should be set up with a minimal set of test-data.
-`GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully.
-If there are any failures then the automated tests fail.
-These tests are run both with the latest available version of `Nextflow` and also the minimum required version that is stated in the pipeline code.
-
-## Patch
-
-:warning: Only in the unlikely and regretful event of a release happening with a bug.
-
-- On your own fork, make a new branch `patch` based on `upstream/main` or `upstream/master`.
-- Fix the bug, and bump version (X.Y.Z+1).
-- Open a pull-request from `patch` to `main`/`master` with the changes.
-
-## Getting help
-
-For further information/help, please consult the [nf-core/sarek documentation](https://nf-co.re/sarek/usage) and don't hesitate to get in touch on the nf-core Slack [#sarek](https://nfcore.slack.com/channels/sarek) channel ([join our Slack here](https://nf-co.re/join/slack)).
-
-## Pipeline contribution conventions
-
-To make the `nf-core/sarek` code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written.
-
-> [!NOTE]
-> For comprehensive, sarek-specific guidance (codebase architecture, channel operations and gotchas, meta map handling, module/subworkflow conventions, and more) see the [Developer Guidelines](../docs/DEVELOPER_GUIDELINES.md). These guidelines are written for both human developers and AI agents.
-
-### Adding a new step
-
-If you wish to contribute a new step, please use the following coding standards:
-
-1. Define the corresponding input channel into your new process from the expected previous process channel.
-2. Write the process block (see below).
-3. Define the output channel if needed (see below).
-4. Add any new parameters to `nextflow.config` with a default (see below).
-5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
-6. Add sanity checks and validation for all relevant parameters.
-7. Perform local tests to validate that the new code works as expected.
-8. If applicable, add a new test in the `tests` directory.
-9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module.
-10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
-
-### Default values
-
-Parameters should be initialised / defined with default values within the `params` scope in `nextflow.config`.
-
-Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`.
-
-### Default processes resource requirements
-
-Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels.
-
-The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block.
-
-### Naming schemes
-
-Please use the following naming schemes, to make it easy to understand what is going where.
-
-- initial process channel: `ch_output_from_<process>`
-- intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
-
-### Nextflow version bumping
-
-If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]`
-
-### Images and figures
-
-For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines).
-
-## GitHub Codespaces
-
-This repo includes a devcontainer configuration which will create a GitHub Codespaces for Nextflow development! This is an online developer environment that runs in your browser, complete with VSCode and a terminal.
-
-To get started:
-
-- Open the repo in [Codespaces](https://github.com/nf-core/sarek/codespaces)
-- Tools installed
-  - nf-core
-  - Nextflow
-
-Devcontainer specs:
-
-- [DevContainer config](.devcontainer/devcontainer.json)
diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md
index a52619f09f..8af2911fb4 100644
--- a/.github/PULL_REQUEST_TEMPLATE.md
+++ b/.github/PULL_REQUEST_TEMPLATE.md
@@ -8,14 +8,14 @@ These are the most common things requested on pull requests (PRs).
 
 Remember that PRs should be made against the dev branch, unless you're preparing a pipeline release.
 
-Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
+Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/docs/CONTRIBUTING.md)
 -->
 
 ## PR checklist
 
 - [ ] This comment contains a description of changes (with reason).
 - [ ] If you've fixed a bug or added code that should be tested, add tests!
-- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
+- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/sarek/tree/master/docs/CONTRIBUTING.md)
 - [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
 - [ ] Make sure your code lints (`nf-core pipelines lint`).
 - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
diff --git a/.github/actions/get-shards/action.yml b/.github/actions/get-shards/action.yml
index 34085279f8..e2833ee979 100644
--- a/.github/actions/get-shards/action.yml
+++ b/.github/actions/get-shards/action.yml
@@ -21,7 +21,7 @@ runs:
   using: "composite"
   steps:
     - name: Install nf-test
-      uses: nf-core/setup-nf-test@v1
+      uses: nf-core/setup-nf-test@4069fbbaabe94c08faba4ad261bfa88225ba133f # v2
       with:
         version: ${{ env.NFT_VER }}
     - name: Get number of shards
diff --git a/.github/workflows/cloudtest.yml b/.github/workflows/awsfulltest.yml
similarity index 91%
rename from .github/workflows/cloudtest.yml
rename to .github/workflows/awsfulltest.yml
index 53d6e5e295..fb4ff59bc6 100644
--- a/.github/workflows/cloudtest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -1,8 +1,7 @@
-name: nf-core cloud tests (both full size and smaller)
+name: nf-core AWS full size tests
 # This workflow is triggered on PRs opened against the main/master branch.
 # It can be additionally triggered manually with GitHub actions workflow dispatch button.
-# It runs the -profile 'test_full' on cloud
-# or the -profile 'test' on cloud (smaller tests)
+# It runs the -profile 'test_full' on AWS batch
 
 on:
   pull_request_review:
@@ -11,10 +10,6 @@ on:
     types: [published]
   workflow_dispatch:
     inputs:
-      test:
-        description: "-profile test (smaller)"
-        type: boolean
-        default: true
       somatic:
         description: "Somatic full test"
         type: boolean
@@ -41,12 +36,6 @@ jobs:
         run: |
           # All available profiles
           all='[
-            {
-              "profile": "test_aws",
-              "test": "test",
-              "compute_env": "TOWER_COMPUTE_ENV",
-              "workdir": "TOWER_BUCKET_AWS"
-            },
             {
               "profile": "test_full_aws",
               "test": "somatic",
@@ -70,7 +59,6 @@ jobs:
           if [[ "${{ github.event_name }}" == "workflow_dispatch" ]]; then
             # Filter to only selected profiles
             matrix=$(echo "$all" | jq -c '[.[] | select(
-              (.test == "test" and ${{ inputs.test }}) or
               (.test == "somatic" and ${{ inputs.somatic }}) or
               (.test == "germline" and ${{ inputs.germline }}) or
               (.test == "germline_ncbench_agilent" and ${{ inputs.germline_ncbench_agilent }})
diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml
new file mode 100644
index 0000000000..33d3bb0aaf
--- /dev/null
+++ b/.github/workflows/awstest.yml
@@ -0,0 +1,33 @@
+name: nf-core AWS test
+# This workflow can be triggered manually with the GitHub actions workflow dispatch button.
+# It runs the -profile 'test' on AWS batch
+
+on:
+  workflow_dispatch:
+jobs:
+  run-platform:
+    name: Run AWS tests
+    if: github.repository == 'nf-core/sarek'
+    runs-on: ubuntu-latest
+    steps:
+      # Launch workflow using Seqera Platform CLI tool action
+      - name: Launch workflow via Seqera Platform
+        uses: seqeralabs/action-tower-launch@51565b514bff1827cf34620de25d0055759f1fc9 # v2
+        with:
+          workspace_id: ${{ vars.TOWER_WORKSPACE_ID }}
+          access_token: ${{ secrets.TOWER_ACCESS_TOKEN }}
+          compute_env: ${{ vars.TOWER_COMPUTE_ENV }}
+          revision: ${{ github.sha }}
+          workdir: s3://${{ vars.AWS_S3_BUCKET }}/work/sarek/work-${{ github.sha }}
+          parameters: |
+            {
+              "outdir": "s3://${{ vars.AWS_S3_BUCKET }}/sarek/results-test-${{ github.sha }}"
+            }
+          profiles: test
+
+      - uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+        with:
+          name: Seqera Platform debug log file
+          path: |
+            tower_action_*.log
+            tower_action_*.json
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index 0bb9eb5738..ce61ff29f5 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -21,7 +21,7 @@ jobs:
       # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
       - name: Post PR comment
         if: failure()
-        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
+        uses: mshick/add-pr-comment@8e4927817251f1ff60c001f04568532b38e0b4a0 # v3
         with:
           message: |
             ## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x:
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index 6adb0fff4b..172de6f373 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@5f858e3efba33a5ca4407a664cc011ad407f2008 # v10
+      - uses: actions/stale@b5d41d4e1d5dceea10e7104786b73624c18a190f # v10
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index 45884ff900..a7bf4fc21f 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -38,13 +38,16 @@ jobs:
     runs-on: ubuntu-latest
     needs: configure
     steps:
+      - name: Check out pipeline code
+        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v2
+        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
 
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
         with:
           python-version: "3.14"
           architecture: "x64"
@@ -54,10 +57,15 @@ jobs:
         with:
           apptainer-version: 1.3.4
 
+      - name: Read .nf-core.yml
+        id: read_yml
+        run: |
+          echo "nf_core_version=$(yq '.nf_core_version' ${{ github.workspace }}/.nf-core.yml)" >> "$GITHUB_OUTPUT"
+
       - name: Install dependencies
         run: |
           python -m pip install --upgrade pip
-          pip install git+https://github.com/nf-core/tools.git
+          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
 
       - name: Make a cache directory for the container images
         run: |
@@ -127,7 +135,7 @@ jobs:
           fi
 
       - name: Upload Nextflow logfile for debugging purposes
-        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
         with:
           name: nextflow_logfile.txt
           path: .nextflow.log*
diff --git a/.github/workflows/fix_linting.yml b/.github/workflows/fix_linting.yml
index 8957338b18..ea83774c5d 100644
--- a/.github/workflows/fix_linting.yml
+++ b/.github/workflows/fix_linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -31,22 +31,18 @@ jobs:
         env:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
-      # Install and run pre-commit
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
-        with:
-          python-version: "3.14"
-
-      - name: Install pre-commit
-        run: pip install pre-commit
+      - name: Install Nextflow
+        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
 
-      - name: Run pre-commit
-        id: pre-commit
-        run: pre-commit run --all-files
+      # Install and run prek
+      - name: Run prek
+        id: prek
+        uses: j178/prek-action@6ad80277337ad479fe43bd70701c3f7f8aa74db3 # v2
         continue-on-error: true
 
       # indication that the linting has finished
       - name: react if linting finished succesfully
-        if: steps.pre-commit.outcome == 'success'
+        if: steps.prek.outcome == 'success'
         uses: peter-evans/create-or-update-comment@e8674b075228eee787fea43ef493e45ece1004c9 # v5
         with:
           comment-id: ${{ github.event.comment.id }}
@@ -54,7 +50,7 @@ jobs:
 
       - name: Commit & push changes
         id: commit-and-push
-        if: steps.pre-commit.outcome == 'failure'
+        if: steps.prek.outcome == 'failure'
         run: |
           git config user.email "core@nf-co.re"
           git config user.name "nf-core-bot"
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 7a527a3464..8738ffc990 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -11,33 +11,31 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
 
-      - name: Set up Python 3.14
-        uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
-        with:
-          python-version: "3.14"
-
-      - name: Install pre-commit
-        run: pip install pre-commit
+      - name: Install Nextflow
+        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
 
-      - name: Run pre-commit
-        run: pre-commit run --all-files
+      - name: Run prek
+        uses: j178/prek-action@6ad80277337ad479fe43bd70701c3f7f8aa74db3 # v2
 
   nf-core:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@v2
+        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
 
-      - uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      - uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
         with:
           python-version: "3.14"
           architecture: "x64"
 
+      - name: Setup uv
+        uses: astral-sh/setup-uv@08807647e7069bb48b6ef5acd8ec9567f424441b # v8.1.0
+
       - name: read .nf-core.yml
         uses: pietrobolcato/action-read-yaml@9f13718d61111b69f30ab4ac683e67a56d254e1d # 1.1.0
         id: read_yml
@@ -45,12 +43,10 @@ jobs:
           config: ${{ github.workspace }}/.nf-core.yml
 
       - name: Install dependencies
-        run: |
-          python -m pip install --upgrade pip
-          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+        run: uv tool install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
 
       - name: Run nf-core pipelines lint
-        if: ${{ github.base_ref != 'master' }}
+        if: ${{ github.base_ref != 'master' || github.base_ref != 'main' }}
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
@@ -58,7 +54,7 @@ jobs:
         run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md
 
       - name: Run nf-core pipelines lint --release
-        if: ${{ github.base_ref == 'master' }}
+        if: ${{ github.base_ref == 'master' || github.base_ref == 'main' }}
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
@@ -71,7 +67,7 @@ jobs:
 
       - name: Upload linting log file artifact
         if: ${{ always() }}
-        uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
         with:
           name: linting-logs
           path: |
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
index e6e9bc269c..5b0c24f751 100644
--- a/.github/workflows/linting_comment.yml
+++ b/.github/workflows/linting_comment.yml
@@ -11,7 +11,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Download lint results
-        uses: dawidd6/action-download-artifact@ac66b43f0e6a346234dd65d4d0c8fbb31cb316e5 # v11
+        uses: dawidd6/action-download-artifact@b6e2e70617bc3265edd6dab6c906732b2f1ae151 # v21
         with:
           workflow: linting.yml
           workflow_conclusion: completed
@@ -21,7 +21,7 @@ jobs:
         run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
 
       - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@773744901bac0e8cbb5a0dc842800d45e9b2b405 # v2
+        uses: marocchino/sticky-pull-request-comment@70d2764d1a7d5d9560b100cbea0077fc8f633987 # v3
         with:
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
           number: ${{ steps.pr_number.outputs.pr_number }}
diff --git a/.github/workflows/nf-test-gpu.yml b/.github/workflows/nf-test-gpu.yml
index 493b61bbf3..e6ee69ddea 100644
--- a/.github/workflows/nf-test-gpu.yml
+++ b/.github/workflows/nf-test-gpu.yml
@@ -18,7 +18,8 @@ concurrency:
 
 env:
   GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-  NFT_VER: "0.9.3"
+  NFT_VER: "0.9.5"
+  NFT_WORKDIR: "~"
   NXF_ANSI_LOG: false
   NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
   NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
@@ -38,7 +39,7 @@ jobs:
           rm -rf ./* || true
           rm -rf ./.??* || true
           ls -la ./
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
@@ -77,14 +78,14 @@ jobs:
           - isMain: false
             profile: "singularity"
         NXF_VER:
-          - 25.10.2
-          - latest-everything
+          - "25.10.4"
+          - "latest-everything"
     env:
       NXF_ANSI_LOG: false
       TOTAL_SHARDS: ${{ needs.nf-test-gpu-changes.outputs.total_shards }}
 
     steps:
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
diff --git a/.github/workflows/nf-test-sentieon.yml b/.github/workflows/nf-test-sentieon.yml
index d67114a1ee..6b7d5de27c 100644
--- a/.github/workflows/nf-test-sentieon.yml
+++ b/.github/workflows/nf-test-sentieon.yml
@@ -18,7 +18,8 @@ concurrency:
 
 env:
   GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-  NFT_VER: "0.9.3"
+  NFT_VER: "0.9.5"
+  NFT_WORKDIR: "~"
   NXF_ANSI_LOG: false
   NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
   NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
@@ -38,7 +39,7 @@ jobs:
           rm -rf ./* || true
           rm -rf ./.??* || true
           ls -la ./
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
@@ -78,14 +79,14 @@ jobs:
           - isMain: false
             profile: "singularity"
         NXF_VER:
-          - 25.10.2
-          - latest-everything
+          - "25.10.4"
+          - "latest-everything"
     env:
       NXF_ANSI_LOG: false
       TOTAL_SHARDS: ${{ needs.nf-test-sentieon-changes.outputs.total_shards }}
 
     steps:
-      - uses: actions/checkout@08c6903cd8c0fde910a37f88322edcfb5dd907a8 # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index b3c8f95059..88ddc03266 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -18,10 +18,12 @@ concurrency:
 
 env:
   GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-  NFT_VER: "0.9.3"
+  NFT_VER: "0.9.5"
+  NFT_WORKDIR: "~"
   NXF_ANSI_LOG: false
   NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity
   NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity
+
 jobs:
   nf-test-changes:
     name: nf-test-changes
@@ -38,7 +40,7 @@ jobs:
           rm -rf ./* || true
           rm -rf ./.??* || true
           ls -la ./
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
@@ -48,8 +50,8 @@ jobs:
         env:
           NFT_VER: ${{ env.NFT_VER }}
         with:
-          tags: "cpu,cpu_conda"
           max_shards: 15
+          tags: "cpu,cpu_conda"
 
       - name: debug
         run: |
@@ -78,20 +80,17 @@ jobs:
           - isMain: false
             profile: "singularity"
         NXF_VER:
-          - 25.10.2
-          - latest-everything
+          - "25.10.4"
+          - "latest-everything"
     env:
       NXF_ANSI_LOG: false
       TOTAL_SHARDS: ${{ needs.nf-test-changes.outputs.total_shards }}
 
     steps:
-      - uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           fetch-depth: 0
 
-      - name: Set NFT_WORKDIR dynamically
-        run: echo "NFT_WORKDIR=$HOME" >> $GITHUB_ENV
-
       - name: Run nf-test
         id: run_nf_test
         uses: ./.github/actions/nf-test
@@ -104,6 +103,7 @@ jobs:
           shard: ${{ matrix.shard }}
           total_shards: ${{ env.TOTAL_SHARDS }}
           tags: ${{ matrix.profile == 'conda' && 'cpu_conda' || 'cpu,cpu_conda' }}
+
       - name: Report test status
         if: ${{ always() }}
         run: |
@@ -118,6 +118,7 @@ jobs:
               exit 1
             fi
           fi
+
   confirm-pass:
     needs: [nf-test]
     if: always()
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 431d3d4457..78d5dbe056 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -18,7 +18,7 @@ jobs:
         id: get_description
         run: |
           echo "description=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .description')" >> $GITHUB_OUTPUT
-      - uses: rzr/fediverse-action@master
+      - uses: rzr/fediverse-action@563159eb8d45f70ab6aaba36ed55cd037e51f441 # master
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
           host: "mstdn.science" # custom host if not "mastodon.social" (default)
@@ -34,7 +34,7 @@ jobs:
   bsky-post:
     runs-on: ubuntu-latest
     steps:
-      - uses: zentered/bluesky-post-action@6461056ea355ea43b977e149f7bf76aaa572e5e8 # v0.3.0
+      - uses: zentered/bluesky-post-action@5a91cc2ad10a304a4e96c16182dbe4918710bcf6 # v0.4.0
         with:
           post: |
             Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!
diff --git a/.github/workflows/template-version-comment.yml b/.github/workflows/template-version-comment.yml
index e8560fc7c9..ea30827ec8 100644
--- a/.github/workflows/template-version-comment.yml
+++ b/.github/workflows/template-version-comment.yml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@93cb6efe18208431cddfb8368fd83d5badbf9bfd # v5
+        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
         with:
           ref: ${{ github.event.pull_request.head.sha }}
 
@@ -29,7 +29,7 @@ jobs:
         run: echo "OUTPUT=$(pip list --outdated | grep nf-core)" >> ${GITHUB_ENV}
 
       - name: Post nf-core template version comment
-        uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2
+        uses: mshick/add-pr-comment@8e4927817251f1ff60c001f04568532b38e0b4a0 # v3
         if: |
           contains(env.OUTPUT, 'nf-core')
         with:
@@ -42,5 +42,5 @@ jobs:
             > Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}.
             > Please update your pipeline to the latest version.
             >
-            > For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync).
+            > For more documentation on how to update your pipeline, please see the [Synchronisation documentation](https://nf-co.re/docs/developing/template-syncs/overview).
           #
diff --git a/.nf-core.yml b/.nf-core.yml
index 8431730ebe..cd09c125f6 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -5,7 +5,6 @@ lint:
     - .github/workflows/ci.yml
     - conf/modules.config
   files_unchanged:
-    - .github/CONTRIBUTING.md
     - .gitignore
     - assets/nf-core-sarek_logo_light.png
     - docs/images/nf-core-sarek_logo_dark.png
@@ -14,11 +13,12 @@ lint:
   nf_test_content: false
   schema_params: false
   template_strings: false
-nf_core_version: 3.5.1
+nf_core_version: 4.0.2
 repository_type: pipeline
 template:
   author: Maxime Garcia, Szilveszter Juhos, Friederike Hanssen
-  description: An open-source analysis pipeline to detect germline or somatic variants from whole genome or targeted sequencing
+  description: An open-source analysis pipeline to detect germline or somatic variants
+    from whole genome or targeted sequencing
   force: false
   is_nfcore: true
   name: sarek
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 517dfd8eab..8b5b7e9436 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -52,6 +52,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 #### Changed
 
+- [#2178](https://github.com/nf-core/sarek/pull/2178) - Template update for nf-core/tools v4.0.2
 - [#2225](https://github.com/nf-core/sarek/pull/2225) - Back to dev (3.9.1dev)
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update nft-utils to 1.0.0, migrate `getAllFilesFromDir` to `getAllFilesFromPath` in test utilities
 
diff --git a/README.md b/README.md
index dd54dedd38..f73c761058 100644
--- a/README.md
+++ b/README.md
@@ -12,7 +12,7 @@
 [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
-[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
+[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
 [![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
@@ -128,7 +128,7 @@ Friederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.
 
 The Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.
 
-Maintenance is now lead by Friederike Hanssen and Maxime U Garcia (now at [Seqera](https://seqera.io))
+Maintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.
 
 Main developers:
 
@@ -207,7 +207,7 @@ We thank the following people for their extensive assistance in the development
 
 If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
 
-For further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek) (you can join with [this invite](https://nf-co.re/join/slack)), or contact us: [Maxime U Garcia](mailto:maxime.garcia@seqera.io?subject=[GitHub]%20nf-core/sarek), [Friederike Hanssen](mailto:friederike.hanssen@qbic.uni-tuebingen.de?subject=[GitHub]%20nf-core/sarek)
+For further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).
 
 ## Citations
 
diff --git a/conf/containers_conda_lock_files_amd64.config b/conf/containers_conda_lock_files_amd64.config
index a05393e6dd..41e234d506 100644
--- a/conf/containers_conda_lock_files_amd64.config
+++ b/conf/containers_conda_lock_files_amd64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-839587b417d23042_1.txt' } }
+process { withName: 'FASTQC' { container = 'modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt' } }
+process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt' } }
diff --git a/conf/containers_conda_lock_files_arm64.config b/conf/containers_conda_lock_files_arm64.config
index 7b7f2b66fc..5b5b9aadf1 100644
--- a/conf/containers_conda_lock_files_arm64.config
+++ b/conf/containers_conda_lock_files_arm64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-3e45d17b40a576b4_1.txt' } }
+process { withName: 'FASTQC' { container = 'modules/nf-core/fastqc/.conda-lock/linux_arm64-bd-e455e32f745abe68_1.txt' } }
+process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt' } }
diff --git a/conf/containers_docker_amd64.config b/conf/containers_docker_amd64.config
index 66a4c027d8..a66e3d1f63 100644
--- a/conf/containers_docker_amd64.config
+++ b/conf/containers_docker_amd64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'community.wave.seqera.io/library/multiqc:1.35--839587b417d23042' } }
+process { withName: 'FASTQC' { container = 'community.wave.seqera.io/library/fastqc:0.12.1--5cb1a2fa2f18c7c2' } }
+process { withName: 'MULTIQC' { container = 'community.wave.seqera.io/library/multiqc:1.35--c17fb751507e9dfc' } }
diff --git a/conf/containers_docker_arm64.config b/conf/containers_docker_arm64.config
index 209188f0d1..215f6865ab 100644
--- a/conf/containers_docker_arm64.config
+++ b/conf/containers_docker_arm64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'community.wave.seqera.io/library/multiqc:1.35--3e45d17b40a576b4' } }
+process { withName: 'FASTQC' { container = 'community.wave.seqera.io/library/fastqc:0.12.1--e455e32f745abe68' } }
+process { withName: 'MULTIQC' { container = 'community.wave.seqera.io/library/multiqc:1.35--5c84a5000a226ab5' } }
diff --git a/conf/containers_singularity_https_amd64.config b/conf/containers_singularity_https_amd64.config
index 2465ec54e7..2fb11a3525 100644
--- a/conf/containers_singularity_https_amd64.config
+++ b/conf/containers_singularity_https_amd64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/12/1297c0f5075c19486da167ebf1b6136907d6b5339697b87b29fda335221785b3/data' } }
+process { withName: 'FASTQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f2/f20b021476d1d87658820f971ebecc1e8cdbde0f338eb0d9cea2b0a8fc54a54b/data' } }
+process { withName: 'MULTIQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c8/c8e346f4f6080eadf1253505e6ff09ef004454fc18e8d672006fd7b222cc412e/data' } }
diff --git a/conf/containers_singularity_https_arm64.config b/conf/containers_singularity_https_arm64.config
index 80ca27ed96..5fa5b4f90a 100644
--- a/conf/containers_singularity_https_arm64.config
+++ b/conf/containers_singularity_https_arm64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c0/c007304153702edc622f1a76b41505e7fca65c7145e5b8b2ddce62a5c59af207/data' } }
+process { withName: 'FASTQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/46/46daf2dad0169afd2ae047c3e50ed3776259f664bf07e5e06b045dc23449e994/data' } }
+process { withName: 'MULTIQC' { container = 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e4/e48aa28aebc881254a499b24c3e1ce77b8df1b85a5432699ed6f72eb17ac7fb5/data' } }
diff --git a/conf/containers_singularity_oras_amd64.config b/conf/containers_singularity_oras_amd64.config
index 19753cfe95..b334375722 100644
--- a/conf/containers_singularity_oras_amd64.config
+++ b/conf/containers_singularity_oras_amd64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'oras://community.wave.seqera.io/library/multiqc:1.35--cb7458fda84d6393' } }
+process { withName: 'FASTQC' { container = 'oras://community.wave.seqera.io/library/fastqc:0.12.1--5c4bd442468d75dd' } }
+process { withName: 'MULTIQC' { container = 'oras://community.wave.seqera.io/library/multiqc:1.35--c680f2aea25ccec2' } }
diff --git a/conf/containers_singularity_oras_arm64.config b/conf/containers_singularity_oras_arm64.config
index e5439f67e5..661c6568e5 100644
--- a/conf/containers_singularity_oras_arm64.config
+++ b/conf/containers_singularity_oras_arm64.config
@@ -1 +1,2 @@
-process { withName: 'MULTIQC' { container = 'oras://community.wave.seqera.io/library/multiqc:1.35--f79e87603d312ac0' } }
+process { withName: 'FASTQC' { container = 'oras://community.wave.seqera.io/library/fastqc:0.12.1--127a87fc06499035' } }
+process { withName: 'MULTIQC' { container = 'oras://community.wave.seqera.io/library/multiqc:1.35--c0468833d65b2f81' } }
diff --git a/docs/CONTRIBUTING.md b/docs/CONTRIBUTING.md
new file mode 100644
index 0000000000..6440b8ffc0
--- /dev/null
+++ b/docs/CONTRIBUTING.md
@@ -0,0 +1,201 @@
+---
+title: Contributing
+markdownPlugin: checklist
+---
+
+# `nf-core/sarek`: Contributing guidelines
+
+Hi there!
+Thanks for taking an interest in improving nf-core/sarek.
+
+This page describes the recommended nf-core way to contribute to both nf-core/sarek and nf-core pipelines in general, including:
+
+- [General contribution guidelines](#general-contribution-guidelines): common procedures or guides across all nf-core pipelines.
+- [Pipeline-specific contribution guidelines](#pipeline-specific-contribution-guidelines): procedures or guides specific to the development conventions of nf-core/sarek.
+
+> [!NOTE]
+> If you need help using or modifying nf-core/sarek, ask on the nf-core Slack [#sarek](https://nfcore.slack.com/channels/sarek) channel ([join our Slack here](https://nf-co.re/join/slack)).
+
+## General contribution guidelines
+
+### Contribution quick start
+
+To contribute code to any nf-core pipeline:
+
+- [ ] Ensure you have Nextflow, nf-core tools, and nf-test installed. See the [nf-core/tools repository](https://github.com/nf-core/tools) for instructions.
+- [ ] Check whether a GitHub [issue](https://github.com/nf-core/sarek/issues) about your idea already exists. If an issue does not exist, create one so that others are aware you are working on it.
+- [ ] [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/sarek repository](https://github.com/nf-core/sarek) to your GitHub account.
+- [ ] Create a branch on your forked repository and make your changes following [pipeline conventions](#pipeline-contribution-conventions) (if applicable).
+- [ ] To fix major bugs, name your branch `patch` and follow the [patch release](#patch-release) process.
+- [ ] Update relevant documentation within the `docs/` folder, use nf-core/tools to update `nextflow_schema.json`, and update `CITATIONS.md`.
+- [ ] Run and/or update tests. See [Testing](#testing) for more information.
+- [ ] [Lint](#lint-tests) your code with nf-core/tools.
+- [ ] Submit a pull request (PR) against the `dev` branch and request a review.
+
+If you are not used to this workflow with Git, see the [GitHub documentation](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or [Git resources](https://try.github.io/) for more information.
+
+## Use of AI and LLMs
+
+The nf-core stance on the use of AI and LLMs is that humans are still ultimately responsible for their submitted code, regardless of the tools they use.
+
+If you’re using AI tools, try to stick by these guidelines:
+
+- Keep PRs as small and focussed as possible
+- Avoid any unnecessary changes, such as moving or refactoring code (unless that is the explicit intention of the PR)
+- Review all generated code yourself before opening a PR, and ensure that you understand it
+- Engage with the community review process and expect to make revisions
+
+For more detail, see the the [blog post](https://nf-co.re/blog/2026/statement-on-ai) for a statement from the nf-core/core team.
+
+### Getting help
+
+For further information and help, see the [nf-core/sarek documentation](https://nf-co.re/sarek/usage) or ask on the nf-core [#sarek](https://nfcore.slack.com/channels/sarek) Slack channel ([join our Slack here](https://nf-co.re/join/slack)).
+
+### GitHub Codespaces
+
+You can contribute to nf-core/sarek without installing a local development environment on your machine by using [GitHub Codespaces](https://github.com/codespaces).
+
+[GitHub Codespaces](https://github.com/codespaces) is an online developer environment that runs in your browser, complete with VS Code and a terminal.
+Most nf-core repositories include a devcontainer configuration, which creates a GitHub Codespaces environment specifically for Nextflow development.
+The environment includes pre-installed nf-core tools, Nextflow, and a few other helpful utilities via a Docker container.
+
+To get started, open the repository in [Codespaces](https://github.com/nf-core/sarek/codespaces).
+
+### Testing
+
+Once you have made your changes, run the pipeline with nf-test to test them locally.
+For additional information, use the `--verbose` flag to view the Nextflow console log output.
+
+```bash
+nf-test test --tag test --profile +docker --verbose
+```
+
+If you have added new functionality, ensure you update the test assertions in the `.nf.test` files in the `tests/` directory.
+Update the snapshots with the following command:
+
+```bash
+nf-test test --tag test --profile +docker --verbose --update-snapshots
+```
+
+When you create a pull request with changes, GitHub Actions will run automatic tests.
+Pull requests are typically reviewed when these tests are passing.
+
+Two types of tests are typically run:
+
+#### Lint tests
+
+nf-core has a [set of guidelines](https://nf-co.re/docs/specifications/overview) which all pipelines must follow.
+To enforce these, run linting with nf-core/tools:
+
+```bash
+nf-core pipelines lint <pipeline_directory>
+```
+
+If you encounter failures or warnings, follow the linked documentation printed to screen.
+For more information about linting tests, see [nf-core/tools API documentation](https://nf-co.re/docs/nf-core-tools/api_reference/latest/pipeline_lint_tests/actions_awsfulltest).
+
+#### Pipeline tests
+
+Each nf-core pipeline should be set up with a minimal set of test data.
+GitHub Actions runs the pipeline on this data to ensure it runs through and exits successfully.
+If there are any failures then the automated tests fail.
+These tests are run with the latest available version of Nextflow and the minimum required version specified in the pipeline code.
+
+### Patch release
+
+> [!WARNING]
+> Only in the unlikely event of a release that contains a critical bug.
+
+- [ ] Create a new branch `patch` on your fork based on `upstream/main` or `upstream/master`.
+- [ ] Fix the bug and use nf-core/tools to bump the version to the next semantic version, for example, `1.2.3` → `1.2.4`.
+- [ ] Open a Pull Request from `patch` directly to `main`/`master` with the changes.
+
+### Pipeline contribution conventions
+
+nf-core semi-standardises how you write code and other contributions to make the nf-core/sarek code and processing logic more understandable for new contributors and to ensure quality.
+
+#### Add a new pipeline step
+
+To contribute a new step to the pipeline, follow the general nf-core coding procedure.
+Please also refer to the [pipeline-specific contribution guidelines](#pipeline-specific-contribution-guidelines):
+
+- [ ] Define the corresponding [input channel](#channel-naming-schemes) into your new process from the expected previous process channel.
+- [ ] Install a module with nf-core/tools, or write a local module (see [default processes resource requirements](#default-processes-resource-requirements)), and add it to the target `<workflow>.nf`.
+- [ ] Define the output channel if needed. Mix the version output channel into `ch_versions` and relevant files into `ch_multiqc`.
+- [ ] Add new or updated parameters to `nextflow.config` with a [default value](#default-parameter-values).
+- [ ] Add new or updated parameters and relevant help text to `nextflow_schema.json` with [nf-core/tools](#default-parameter-values).
+- [ ] Add validation for relevant parameters to the pipeline utilisation section of `utils_nfcore_\_pipeline/main.nf` subworkflow.
+- [ ] Perform local tests to validate that the new code works as expected.
+  - [ ] If applicable, add a new test in the `tests` directory.
+- [ ] Update `usage.md`, `output.md`, and `citation.md` as appropriate.
+- [ ] [Lint](lint) the code with nf-core/tools.
+- [ ] Update any diagrams or pipeline images as necessary.
+- [ ] Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name cleanup, and module plots are in the appropriate order.
+- [ ] If applicable, create a [MultiQC](https://seqera.io/multiqc/) module.
+- [ ] Add a description of the output files and, if relevant, images from the MultiQC report to `docs/output.md`.
+
+To update the minimum required Nextflow version, see the [Nextflow version bumping](#nextflow-version-bumping) section below. For more information about pipeline contributions, see [pipeline-specific contribution guidelines](#pipeline-specific-contribution-guidelines).
+
+#### Channel naming schemes
+
+Use the following naming schemes for channels to make the channel flow easier to understand:
+
+- Initial process channel: `ch_output_from_<process>`
+- Intermediate and terminal channels: `ch_<previousprocess>_for_<nextprocess>`
+
+#### Default parameter values
+
+Parameters should be initialised and defined with default values within the `params` scope in `nextflow.config`.
+They should also be documented in the pipeline JSON schema.
+
+To update `nextflow_schema.json`, run:
+
+```bash
+nf-core pipelines schema build
+```
+
+The schema builder interface that loads in your browser should automatically update the defaults in the parameter documentation.
+
+#### Default processes resource requirements
+
+If you write a local module, specify a default set of resource requirements for the process.
+
+Sensible defaults for process resource requirements (CPUs, memory, time) should be defined in `conf/base.config`.
+Specify these with generic `withLabel:` selectors, so they can be shared across multiple processes and steps of the pipeline.
+
+nf-core provides a set of standard labels that you should follow where possible, as seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config).
+These labels define resource defaults for single-core processes, modules that require a GPU, and different levels of multi-core configurations with increasing memory requirements.
+
+Values assigned within these labels can be dynamically passed to a tool using the the `${task.cpus}` and `${task.memory}` Nextflow variables in the `script:` block of a module (see an example in the [modules repository](https://github.com/nf-core/modules/blob/bd1b6a40f55933d94b8c9ca94ec8c1ea0eaf4b82/modules/nf-core/samtools/bam2fq/main.nf#L30)).
+
+#### Nextflow version bumping
+
+If you use a new feature from core Nextflow, bump the minimum required Nextflow version in the pipeline with:
+
+```bash
+nf-core pipelines bump-version --nextflow . <min_nf_version>
+```
+
+#### Images and figures guidelines
+
+If you update images or graphics, follow the nf-core [style guidelines](https://nf-co.re/docs/community/brand/workflow-schematics).
+
+## Pipeline specific contribution guidelines
+
+> [!NOTE]
+> For comprehensive, sarek-specific guidance (codebase architecture, channel operations and gotchas, meta map handling, module/subworkflow conventions, and more) see the [Developer Guidelines](../docs/DEVELOPER_GUIDELINES.md). These guidelines are written for both human developers and AI agents.
+
+### Adding a new step
+
+If you wish to contribute a new step, please use the following coding standards:
+
+1. Define the corresponding input channel into your new process from the expected previous process channel.
+2. Write the process block (see below).
+3. Define the output channel if needed (see below).
+4. Add any new parameters to `nextflow.config` with a default (see below).
+5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
+6. Add sanity checks and validation for all relevant parameters.
+7. Perform local tests to validate that the new code works as expected.
+8. If applicable, add a new test in the `tests` directory.
+9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://multiqc.info/) module.
+10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
diff --git a/modules.json b/modules.json
index c77387364e..d2272c9181 100644
--- a/modules.json
+++ b/modules.json
@@ -178,7 +178,7 @@
                     },
                     "fastqc": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "fgbio/callmolecularconsensusreads": {
@@ -388,7 +388,7 @@
                     },
                     "multiqc": {
                         "branch": "master",
-                        "git_sha": "cd0ad387832473916b1f1c80c2ca673a7883bf94",
+                        "git_sha": "98403d15b0e50edae1f3fec5eae5e24982f1fade",
                         "installed_by": ["modules"]
                     },
                     "muse/call": {
diff --git a/modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt b/modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt
new file mode 100644
index 0000000000..7770ccd5fc
--- /dev/null
+++ b/modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt
@@ -0,0 +1,822 @@
+
+version: 6
+environments:
+default:
+channels:
+- url: https://conda.anaconda.org/conda-forge/
+- url: https://conda.anaconda.org/bioconda/
+- url: https://conda.anaconda.org/bioconda/
+options:
+pypi-prerelease-mode: if-necessary-or-explicit
+packages:
+linux-64:
+- conda: https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-20_gnu.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/alsa-lib-1.2.15.3-hb03c661_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.2.25-hbd8a1cb_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/cairo-1.18.4-he90730b_1.conda
+- conda: https://conda.anaconda.org/bioconda/noarch/fastqc-0.12.1-hdfd78af_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.17.1-h27c8c51_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-ecosystem-1-0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/giflib-5.2.2-hd590300_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/graphite2-1.3.14-hecca717_2.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/harfbuzz-13.2.1-h6083320_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/icu-78.3-h33c6efd_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/keyutils-1.6.3-hb9d3cd8_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/krb5-1.22.2-ha1258a1_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/lcms2-2.18-h0c24ade_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/lerc-4.1.0-hdb68285_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libcups-2.3.3-h7a8fb5f_6.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libdeflate-1.25-h17f619e_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libedit-3.1.20250104-pl5321h7949ede_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.7.4-hecca717_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.5.2-h3435931_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype-2.14.3-ha770c72_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype6-2.14.3-h73754d4_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-15.2.0-he0feb66_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-ng-15.2.0-h69a702a_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libglib-2.86.4-h6548e54_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgomp-15.2.0-he0feb66_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libiconv-1.18-h3b78370_2.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libjpeg-turbo-3.1.2-hb03c661_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/liblzma-5.8.2-hb03c661_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libpng-1.6.55-h421ea60_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libstdcxx-15.2.0-h934c35e_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libtiff-4.7.1-h9d88235_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libuuid-2.41.3-h5347b49_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libwebp-base-1.6.0-hd42ef1d_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libxcb-1.17.0-h8a09558_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libxcrypt-4.4.36-hd590300_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libzlib-1.3.2-h25fd6f3_2.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/ncurses-6.5-h2d0b736_3.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/openjdk-25.0.2-ha668962_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/openssl-3.6.1-h35e630c_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/pcre2-10.47-haa7fec5_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/perl-5.32.1-7_hd590300_perl5.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/pixman-0.46.4-h54a6638_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/procps-ng-4.0.6-h18c060e_0.conda
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index 0000000000..cdc434cad3
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diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf
index 23e16634c3..10851264c1 100644
--- a/modules/nf-core/fastqc/main.nf
+++ b/modules/nf-core/fastqc/main.nf
@@ -1,37 +1,40 @@
 process FASTQC {
     tag "${meta.id}"
-    label 'process_medium'
+    label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' :
-        'biocontainers/fastqc:0.12.1--hdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0'
+        : 'quay.io/biocontainers/fastqc:0.12.1--hdfd78af_0'}"
 
     input:
-    tuple val(meta), path(reads)
+    tuple val(meta), path(reads, stageAs: '?/*')
 
     output:
     tuple val(meta), path("*.html"), emit: html
-    tuple val(meta), path("*.zip") , emit: zip
-    path  "versions.yml"           , emit: versions
+    tuple val(meta), path("*.zip"), emit: zip
+    tuple val("${task.process}"), val('fastqc'), eval('fastqc --version | sed "/FastQC v/!d; s/.*v//"'), emit: versions_fastqc, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args          = task.ext.args ?: ''
-    def prefix        = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
     // Make list of old name and new name pairs to use for renaming in the bash while loop
-    def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] }
-    def rename_to     = old_new_pairs*.join(' ').join(' ')
-    def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ')
+    def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[reads, "${prefix}.${reads.extension}"]] : reads.withIndex().collect { entry, index -> [entry, "${prefix}_${index + 1}.${entry.extension}"] }
+    def rename_to = old_new_pairs*.join(' ').join(' ')
+    def renamed_files = old_new_pairs.collect { _old_name, new_name -> new_name }.join(' ')
 
     // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory)
     // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222
-    // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label
-    def memory_in_mb = task.memory ? task.memory.toUnit('MB') / task.cpus : null
+    // Dividing the task.memory by task.cpus allows to stick to requested amount of RAM in the label
+    def memory_in_mb = task.memory
+        ? (task.memory.toUnit('MB') / task.cpus).intValue()
+        : null
     // FastQC memory value allowed range (100 - 10000)
     def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb)
+    def fastqc_memory_arg = fastqc_memory ? "--memory ${fastqc_memory}" : ''
 
     """
     printf "%s %s\\n" ${rename_to} | while read old_name new_name; do
@@ -41,13 +44,8 @@ process FASTQC {
     fastqc \\
         ${args} \\
         --threads ${task.cpus} \\
-        --memory ${fastqc_memory} \\
+        ${fastqc_memory_arg} \\
         ${renamed_files}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
-    END_VERSIONS
     """
 
     stub:
@@ -55,10 +53,5 @@ process FASTQC {
     """
     touch ${prefix}.html
     touch ${prefix}.zip
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml
index c8d9d025ac..2f6cfef6d0 100644
--- a/modules/nf-core/fastqc/meta.yml
+++ b/modules/nf-core/fastqc/meta.yml
@@ -53,13 +53,28 @@ output:
           description: FastQC report archive
           pattern: "*_{fastqc.zip}"
           ontologies: []
+  versions_fastqc:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fastqc:
+          type: string
+          description: The tool name
+      - fastqc --version | sed "/FastQC v/!d; s/.*v//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fastqc:
+          type: string
+          description: The tool name
+      - fastqc --version | sed "/FastQC v/!d; s/.*v//":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@drpatelh"
   - "@grst"
@@ -70,3 +85,27 @@ maintainers:
   - "@grst"
   - "@ewels"
   - "@FelixKrueger"
+containers:
+  docker:
+    linux/arm64:
+      name: community.wave.seqera.io/library/fastqc:0.12.1--e455e32f745abe68
+      build_id: bd-e455e32f745abe68_1
+      scan_id: sc-f102f736465af88c_1
+    linux/amd64:
+      name: community.wave.seqera.io/library/fastqc:0.12.1--5cb1a2fa2f18c7c2
+      build_id: bd-5cb1a2fa2f18c7c2_1
+      scan_id: sc-0c0466326b6b77d2_1
+  singularity:
+    linux/amd64:
+      name: oras://community.wave.seqera.io/library/fastqc:0.12.1--5c4bd442468d75dd
+      build_id: bd-5c4bd442468d75dd_1
+      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f2/f20b021476d1d87658820f971ebecc1e8cdbde0f338eb0d9cea2b0a8fc54a54b/data
+    linux/arm64:
+      name: oras://community.wave.seqera.io/library/fastqc:0.12.1--127a87fc06499035
+      build_id: bd-127a87fc06499035_1
+      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/46/46daf2dad0169afd2ae047c3e50ed3776259f664bf07e5e06b045dc23449e994/data
+  conda:
+    linux/amd64:
+      lock_file: modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt
+    linux/arm64:
+      lock_file: modules/nf-core/fastqc/.conda-lock/linux_arm64-bd-e455e32f745abe68_1.txt
diff --git a/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-839587b417d23042_1.txt b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt
similarity index 92%
rename from modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-839587b417d23042_1.txt
rename to modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt
index 33a1c6925a..2a91c22d61 100644
--- a/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-839587b417d23042_1.txt
+++ b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt
@@ -17,27 +17,27 @@ linux-64:
 - conda: https://conda.anaconda.org/conda-forge/noarch/backports.zstd-1.5.0-py314h680f03e_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-python-1.2.0-py314h3de4e8d_1.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.4.22-hbd8a1cb_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.4.22-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.5.20-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.7-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/click-8.4.0-pyhc90fa1f_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/coloredlogs-15.0.1-pyhd8ed1ab_4.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/colormath-3.0.0-pyhd8ed1ab_4.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.4-py314hd8ed1ab_100.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.8.0-hecca717_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.5-py314hd8ed1ab_100.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.8.1-hecca717_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.17.1-h27c8c51_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.18.0-h27c8c51_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/h2-4.3.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/hpack-4.1.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/humanfriendly-10.0-pyh707e725_8.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/humanize-4.15.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/hyperframe-6.1.0-pyhd8ed1ab_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.13-pyhcf101f3_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-8.8.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.15-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-9.0.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jinja2-3.1.6-pyhcf101f3_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-4.26.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-specifications-2025.9.1-pyhcf101f3_0.conda
@@ -48,7 +48,7 @@ linux-64:
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libblas-3.11.0-7_h4a7cf45_openblas.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libcblas-3.11.0-7_h0358290_openblas.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libdeflate-1.25-h17f619e_0.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.8.0-hecca717_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.8.1-hecca717_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.5.2-h3435931_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype-2.14.3-ha770c72_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype6-2.14.3-h73754d4_0.conda
@@ -75,21 +75,22 @@ linux-64:
 - conda: https://conda.anaconda.org/conda-forge/linux-64/markupsafe-3.0.3-py314h67df5f8_1.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/mathjax-2.7.7-ha770c72_3.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/mdurl-0.1.2-pyhd8ed1ab_1.conda
-- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_0.conda
+- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/narwhals-2.21.2-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/natsort-8.4.0-pyhcf101f3_2.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/ncurses-6.6-hdb14827_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/networkx-3.6.1-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/nspr-4.38-h29cc59b_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/nss-3.118-h445c969_0.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/numpy-2.4.5-py314h2b28147_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/numpy-2.4.6-py314h2b28147_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/openjpeg-2.5.4-h55fea9a_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/openssl-3.6.2-h35e630c_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/packaging-26.2-pyhc364b38_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/pillow-12.2.0-py314h8ec4b1a_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/plotly-6.6.0-pyhd8ed1ab_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.40.1-pyh58ad624_1.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-32-1.40.1-py310h49dadd8_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.41.0-pyh58ad624_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-32-1.41.0-py310h49dadd8_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-compat-1.41.0-py310hcbd6021_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/procps-ng-4.0.6-h18c060e_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/pthread-stubs-0.4-hb9d3cd8_1002.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pyaml-env-1.2.2-pyhd8ed1ab_0.conda
@@ -97,9 +98,9 @@ linux-64:
 - conda: https://conda.anaconda.org/conda-forge/linux-64/pydantic-core-2.46.4-py314h2e6c369_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pygments-2.20.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pysocks-1.7.1-pyha55dd90_7.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.14.4-habeac84_100_cp314.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.14.5-habeac84_100_cp314.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-dotenv-1.2.2-pyhcf101f3_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.4-h4df99d1_100.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.5-h4df99d1_100.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-kaleido-0.2.1-pyhd8ed1ab_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/python_abi-3.14-8_cp314.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-64/pyyaml-6.0.3-py314h67df5f8_1.conda
@@ -205,22 +206,22 @@ license: bzip2-1.0.6
 license_family: BSD
 size: 260182
 timestamp: 1771350215188
-- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.4.22-hbd8a1cb_0.conda
-sha256: c9dbcc8039a52023660d6d1bbf87594a93dd69c6ac5a2a44323af2c92976728d
-md5: e18ad67cf881dcadee8b8d9e2f8e5f73
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
+sha256: 9812a303a1395e1dafbd92e5bc8a1ff6013bcbba0a09c7f03a8d23e43560aa9b
+md5: 489b8e97e666c93f68fdb35c3c9b957f
 depends:
 - __unix
 license: ISC
-size: 131039
-timestamp: 1776865545798
-- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.4.22-pyhd8ed1ab_0.conda
-sha256: 989db6e5957c4b44fa600c68c681ec2f36a55e48f7c7f1c073d5e91caa8cd878
-md5: 929471569c93acefb30282a22060dcd5
+size: 129868
+timestamp: 1779289852439
+- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.5.20-pyhd8ed1ab_0.conda
+sha256: 645655a3510e38e625da136595f3f16f2130c3263630cc3bc8f60f619ddbe490
+md5: 9fefff2f745ea1cc2ef15211a20c054a
 depends:
 - python >=3.10
 license: ISC
-size: 135656
-timestamp: 1776866680878
+size: 134201
+timestamp: 1779285131141
 - conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.7-pyhd8ed1ab_0.conda
 sha256: 3f9483d62ce24ecd063f8a5a714448445dc8d9e201147c46699fc0033e824457
 md5: a9167b9571f3baa9d448faa2139d1089
@@ -238,6 +239,7 @@ depends:
 - python
 - python >=3.10
 license: BSD-3-Clause
+license_family: BSD
 size: 104631
 timestamp: 1779108494556
 - conda: https://conda.anaconda.org/conda-forge/noarch/coloredlogs-15.0.1-pyhd8ed1ab_4.conda
@@ -261,27 +263,27 @@ license: BSD-3-Clause
 license_family: BSD
 size: 39326
 timestamp: 1735759976140
-- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.4-py314hd8ed1ab_100.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.5-py314hd8ed1ab_100.conda
 noarch: generic
-sha256: 40dc224f2b718e5f034efd2332bc315a719063235f63673468d26a24770094ee
-md5: f111d4cfaf1fe9496f386bc98ae94452
+sha256: 777882d2685f368417f31bbe1b28f73687fc6c8f6a5768bda20ffeefa6b07f5b
+md5: a749029ce5d0632a913db19d17f944ab
 depends:
 - python >=3.14,<3.15.0a0
 - python_abi * *_cp314
 license: Python-2.0
-size: 49809
-timestamp: 1775614256655
-- conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.8.0-hecca717_0.conda
-sha256: ca4dc1da00a8aaa56c1088e7f45f1859ecea6f75874e67584f1af6e5cf8179f8
-md5: 992e529e407c9d67d50be1d7543fde4c
+size: 50212
+timestamp: 1779236682725
+- conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.8.1-hecca717_0.conda
+sha256: 29a10599d56d93bd750914888ebe6822d47722070762b4647b34d12df9f4476e
+md5: d0757fd84af06f065eba49d39af6c546
 depends:
 - __glibc >=2.17,<3.0.a0
-- libexpat 2.8.0 hecca717_0
+- libexpat 2.8.1 hecca717_0
 - libgcc >=14
 license: MIT
 license_family: MIT
-size: 148114
-timestamp: 1777846120303
+size: 148238
+timestamp: 1779278694477
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
 sha256: 58d7f40d2940dd0a8aa28651239adbf5613254df0f75789919c4e6762054403b
 md5: 0c96522c6bdaed4b1566d11387caaf45
@@ -310,21 +312,21 @@ license: LicenseRef-Ubuntu-Font-Licence-Version-1.0
 license_family: Other
 size: 1620504
 timestamp: 1727511233259
-- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.17.1-h27c8c51_0.conda
-sha256: aa4a44dba97151221100a637c7f4bde619567afade9c0265f8e1c8eed8d7bd8c
-md5: 867127763fbe935bab59815b6e0b7b5c
+- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.18.0-h27c8c51_0.conda
+sha256: e798086d8a65d55dc4c51f5746705639c9a5f2eeb0b8fc50e6152cfc0d69a4e8
+md5: 06965b2f9854d0b15e0443ee81fe83dc
 depends:
 - __glibc >=2.17,<3.0.a0
-- libexpat >=2.7.4,<3.0a0
-- libfreetype >=2.14.1
-- libfreetype6 >=2.14.1
+- libexpat >=2.8.1,<3.0a0
+- libfreetype >=2.14.3
+- libfreetype6 >=2.14.3
 - libgcc >=14
-- libuuid >=2.41.3,<3.0a0
-- libzlib >=1.3.1,<2.0a0
+- libuuid >=2.42.1,<3.0a0
+- libzlib >=1.3.2,<2.0a0
 license: MIT
 license_family: MIT
-size: 270705
-timestamp: 1771382710863
+size: 280882
+timestamp: 1779421631622
 - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
 sha256: 54eea8469786bc2291cc40bca5f46438d3e062a399e8f53f013b6a9f50e98333
 md5: a7970cd949a077b7cb9696379d338681
@@ -386,27 +388,26 @@ license: MIT
 license_family: MIT
 size: 17397
 timestamp: 1737618427549
-- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.13-pyhcf101f3_0.conda
-sha256: 9ab620e6f64bb67737bd7bc1ad6f480770124e304c6710617aba7fe60b089f48
-md5: fb7130c190f9b4ec91219840a05ba3ac
+- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.15-pyhcf101f3_0.conda
+sha256: 3d25f9f6f7ab3e1ce6429fc8c8aae0335cf446692e715068488536d220cc43de
+md5: 1b9083b7f00609605d1483dbc6071a81
 depends:
 - python >=3.10
 - python
 license: BSD-3-Clause
 license_family: BSD
-size: 59038
-timestamp: 1776947141407
-- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-8.8.0-pyhcf101f3_0.conda
-sha256: 82ab2a0d91ca1e7e63ab6a4939356667ef683905dea631bc2121aa534d347b16
-md5: 080594bf4493e6bae2607e65390c520a
+size: 62642
+timestamp: 1779294335905
+- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-9.0.0-pyhcf101f3_0.conda
+sha256: 43e2a5497cad1598ff88a3e69f69bc88b7b8f141fa63c60eab5db296317318b8
+md5: ffc17e785d64e12fc311af9184221839
 depends:
 - python >=3.10
 - zipp >=3.20
 - python
 license: Apache-2.0
-license_family: APACHE
-size: 34387
-timestamp: 1773931568510
+size: 34766
+timestamp: 1779714582554
 - conda: https://conda.anaconda.org/conda-forge/noarch/jinja2-3.1.6-pyhcf101f3_1.conda
 sha256: fc9ca7348a4f25fed2079f2153ecdcf5f9cf2a0bc36c4172420ca09e1849df7b
 md5: 04558c96691bed63104678757beb4f8d
@@ -536,18 +537,18 @@ license: MIT
 license_family: MIT
 size: 73490
 timestamp: 1761979956660
-- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.8.0-hecca717_0.conda
-sha256: ea33c40977ea7a2c3658c522230058395bc2ee0d89d99f0711390b6a1ee80d12
-md5: a3b390520c563d78cc58974de95a03e5
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.8.1-hecca717_0.conda
+sha256: 363018b25fdb5534c79783d912bd4b685a3547f4fc5996357ad548899b0ee8e7
+md5: 93764a5ca80616e9c10106cdaec92f74
 depends:
 - __glibc >=2.17,<3.0.a0
 - libgcc >=14
 constrains:
-- expat 2.8.0.*
+- expat 2.8.1.*
 license: MIT
 license_family: MIT
-size: 77241
-timestamp: 1777846112704
+size: 77294
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 - conda: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.5.2-h3435931_0.conda
 sha256: 31f19b6a88ce40ebc0d5a992c131f57d919f73c0b92cd1617a5bec83f6e961e6
 md5: a360c33a5abe61c07959e449fa1453eb
@@ -749,6 +750,7 @@ depends:
 - __glibc >=2.17,<3.0.a0
 - libgcc >=14
 license: BSD-3-Clause
+license_family: BSD
 size: 40163
 timestamp: 1779118517630
 - conda: https://conda.anaconda.org/conda-forge/linux-64/libwebp-base-1.6.0-hd42ef1d_0.conda
@@ -838,9 +840,9 @@ license: MIT
 license_family: MIT
 size: 14465
 timestamp: 1733255681319
-- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_0.conda
-sha256: 5267ddcc9119b8bdfdddd83fa4713edf6e956443f7369ebd686dc6d9f921a428
-md5: d95457757ad503af93145630ec6e8965
+- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_1.conda
+sha256: e86033aa55a9e915e2d0957e770bdb81e3feb26a227d1adb17f9d6c528da6a71
+md5: cdb20309681ba3ce8f52c110e214d4f3
 depends:
 - click
 - coloredlogs
@@ -855,6 +857,7 @@ depends:
 - pillow >=10.2.0
 - plotly >=5.18
 - polars >=1.34.0
+- polars-runtime-compat >=1.34.0
 - pyaml-env
 - pydantic >=2.7.1
 - python >=3.9,!=3.14.1
@@ -870,8 +873,8 @@ depends:
 - typeguard >=4
 license: GPL-3.0-or-later
 license_family: GPL3
-size: 4229065
-timestamp: 1778635617929
+size: 4282188
+timestamp: 1779465338806
 - conda: https://conda.anaconda.org/conda-forge/noarch/narwhals-2.21.2-pyhcf101f3_0.conda
 sha256: 70f43d62450927d51673eecd8823e14f5b3cfebdb43cda1d502eba97162bab42
 md5: 6687827c332121727ce383919e1ec8c2
@@ -941,9 +944,9 @@ license: MPL-2.0
 license_family: MOZILLA
 size: 2057773
 timestamp: 1763485556350
-- conda: https://conda.anaconda.org/conda-forge/linux-64/numpy-2.4.5-py314h2b28147_0.conda
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-md5: 64a8d5cd0553d51590a304a28c184785
+- conda: https://conda.anaconda.org/conda-forge/linux-64/numpy-2.4.6-py314h2b28147_0.conda
+sha256: bc61ae892973751a6b0e6ecea57ed6d7053224bddcb007165d6ceb1d7344ad47
+md5: f49b5f950379e0b97c35ca97682f7c6a
 depends:
 - python
 - libstdcxx >=14
@@ -957,8 +960,8 @@ constrains:
 - numpy-base <0a0
 license: BSD-3-Clause
 license_family: BSD
-size: 8930431
-timestamp: 1778894356567
+size: 8928909
+timestamp: 1779169198391
 - conda: https://conda.anaconda.org/conda-forge/linux-64/openjpeg-2.5.4-h55fea9a_0.conda
 sha256: 3900f9f2dbbf4129cf3ad6acf4e4b6f7101390b53843591c53b00f034343bc4d
 md5: 11b3379b191f63139e29c0d19dee24cd
@@ -1028,11 +1031,11 @@ license: MIT
 license_family: MIT
 size: 5251872
 timestamp: 1772628857717
-- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.40.1-pyh58ad624_1.conda
-sha256: 83e37ede46e8e57d4785e804604a88fa02d6eac9a774cee40d49018f9da9ead1
-md5: bdbac766376390889b74216b70206af4
+- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.41.0-pyh58ad624_0.conda
+sha256: 70fc56877c4a095ee658d61924d8019768fbae4a48437058d181fc94b0a7c4d8
+md5: 25a883fed9f1f3f21ff317a3e7c92ac4
 depends:
-- polars-runtime-32 ==1.40.1
+- polars-runtime-32 ==1.41.0
 - python >=3.10
 - python
 constrains:
@@ -1046,30 +1049,44 @@ constrains:
 - pyiceberg >=0.7.1
 - altair >=5.4.0
 - great_tables >=0.8.0
-- polars-runtime-32 ==1.40.1
-- polars-runtime-64 ==1.40.1
-- polars-runtime-compat ==1.40.1
+- polars-runtime-32 ==1.41.0
+- polars-runtime-64 ==1.41.0
+- polars-runtime-compat ==1.41.0
 license: MIT
-license_family: MIT
-size: 539000
-timestamp: 1778779696741
-- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-32-1.40.1-py310h49dadd8_1.conda
+size: 539656
+timestamp: 1779630790562
+- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-32-1.41.0-py310h49dadd8_0.conda
 noarch: python
-sha256: c163631030b3c53174019e577ffaa9c83dab3bbaa1e6336001c5ed2faae364ba
-md5: 5072dd8db4336777a95a1dd12e24623a
+sha256: e51ee3fe5259f2e115b2f78f8fbe3554e419c7c82b0c110878e12a5ff95ce3ab
+md5: 7682765a1588e5ac887c99736d297c93
 depends:
 - python
+- __glibc >=2.17,<3.0.a0
+- libstdcxx >=14
 - libgcc >=14
+- _python_abi3_support 1.*
+- cpython >=3.10
+constrains:
+- __glibc >=2.17
+license: MIT
+size: 42578921
+timestamp: 1779630790562
+- conda: https://conda.anaconda.org/conda-forge/linux-64/polars-runtime-compat-1.41.0-py310hcbd6021_0.conda
+noarch: python
+sha256: 29c3831c92394af11d9f7d04882dda9479ffbb76a3d36ba155d52159d67805fa
+md5: cb0b620c9914a07a9022cb8b183ea9ee
+depends:
+- python
 - libstdcxx >=14
+- libgcc >=14
 - __glibc >=2.17,<3.0.a0
 - _python_abi3_support 1.*
 - cpython >=3.10
 constrains:
 - __glibc >=2.17
 license: MIT
-license_family: MIT
-size: 42001857
-timestamp: 1778779696741
+size: 41864944
+timestamp: 1779630722548
 - conda: https://conda.anaconda.org/conda-forge/linux-64/procps-ng-4.0.6-h18c060e_0.conda
 sha256: 4ce2e1ee31a6217998f78c31ce7dc0a3e0557d9238b51d49dd20c52d467a126d
 md5: f2c23a77b25efcad57d377b34bd84941
@@ -1149,23 +1166,23 @@ license: BSD-3-Clause
 license_family: BSD
 size: 21085
 timestamp: 1733217331982
-- conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.14.4-habeac84_100_cp314.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/python-3.14.5-habeac84_100_cp314.conda
 build_number: 100
-sha256: dec247c5badc811baa34d6085df9d0465535883cf745e22e8d79092ad54a3a7b
-md5: a443f87920815d41bfe611296e507995
+sha256: 55eed9bf2a3f6e90311276f0834737fe7c2d9ec3e5e2e557507858df4c7521e6
+md5: da92e59ff92f2d5ede4f612af20f583f
 depends:
 - __glibc >=2.17,<3.0.a0
 - bzip2 >=1.0.8,<2.0a0
 - ld_impl_linux-64 >=2.36.1
-- libexpat >=2.7.5,<3.0a0
+- libexpat >=2.8.0,<3.0a0
 - libffi >=3.5.2,<3.6.0a0
 - libgcc >=14
-- liblzma >=5.8.2,<6.0a0
+- liblzma >=5.8.3,<6.0a0
 - libmpdec >=4.0.0,<5.0a0
-- libsqlite >=3.52.0,<4.0a0
-- libuuid >=2.42,<3.0a0
+- libsqlite >=3.53.1,<4.0a0
+- libuuid >=2.42.1,<3.0a0
 - libzlib >=1.3.2,<2.0a0
-- ncurses >=6.5,<7.0a0
+- ncurses >=6.6,<7.0a0
 - openssl >=3.5.6,<4.0a0
 - python_abi 3.14.* *_cp314
 - readline >=8.3,<9.0a0
@@ -1173,8 +1190,8 @@ depends:
 - tzdata
 - zstd >=1.5.7,<1.6.0a0
 license: Python-2.0
-size: 36705460
-timestamp: 1775614357822
+size: 36745188
+timestamp: 1779236923603
 python_site_packages_path: lib/python3.14/site-packages
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-dotenv-1.2.2-pyhcf101f3_0.conda
 sha256: 74e417a768f59f02a242c25e7db0aa796627b5bc8c818863b57786072aeb85e5
@@ -1185,15 +1202,15 @@ license: BSD-3-Clause
 license_family: BSD
 size: 27848
 timestamp: 1772388605021
-- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.4-h4df99d1_100.conda
-sha256: 36ff7984e4565c85149e64f8206303d412a0652e55cf806dcb856903fa056314
-md5: e4e60721757979d01d3964122f674959
+- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.5-h4df99d1_100.conda
+sha256: 41dd7da285d71d519257fa7dacb1cae060d5ebfaa5f92cba5994899d2978e943
+md5: 41954747ba952ec4b01e16c2c9e8d8ff
 depends:
-- cpython 3.14.4.*
+- cpython 3.14.5.*
 - python_abi * *_cp314
 license: Python-2.0
-size: 49806
-timestamp: 1775614307464
+size: 50212
+timestamp: 1779236703009
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-kaleido-0.2.1-pyhd8ed1ab_0.tar.bz2
 sha256: e17bf63a30aec33432f1ead86e15e9febde9fc40a7f869c0e766be8d2db44170
 md5: 310259a5b03ff02289d7705f39e2b1d2
diff --git a/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-3e45d17b40a576b4_1.txt b/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt
similarity index 92%
rename from modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-3e45d17b40a576b4_1.txt
rename to modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt
index 492ce48050..3d5b93db98 100644
--- a/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-3e45d17b40a576b4_1.txt
+++ b/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt
@@ -17,27 +17,27 @@ linux-aarch64:
 - conda: https://conda.anaconda.org/conda-forge/noarch/backports.zstd-1.5.0-py314h680f03e_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/brotli-python-1.2.0-py314h352cb57_1.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/bzip2-1.0.8-h4777abc_9.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.4.22-hbd8a1cb_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.4.22-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.5.20-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.7-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/click-8.4.0-pyhc90fa1f_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/coloredlogs-15.0.1-pyhd8ed1ab_4.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/colormath-3.0.0-pyhd8ed1ab_4.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.4-py314hd8ed1ab_100.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/expat-2.8.0-hfae3067_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.5-py314hd8ed1ab_100.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/expat-2.8.1-hfae3067_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/fontconfig-2.17.1-hba86a56_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/fontconfig-2.18.0-hba86a56_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/h2-4.3.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/hpack-4.1.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/humanfriendly-10.0-pyh707e725_8.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/humanize-4.15.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/hyperframe-6.1.0-pyhd8ed1ab_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.13-pyhcf101f3_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-8.8.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.15-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-9.0.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jinja2-3.1.6-pyhcf101f3_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-4.26.0-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-specifications-2025.9.1-pyhcf101f3_0.conda
@@ -48,7 +48,7 @@ linux-aarch64:
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libblas-3.11.0-7_haddc8a3_openblas.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libcblas-3.11.0-7_hd72aa62_openblas.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libdeflate-1.25-h1af38f5_0.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libexpat-2.8.0-hfae3067_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libexpat-2.8.1-hfae3067_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libffi-3.5.2-h376a255_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libfreetype-2.14.3-h8af1aa0_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libfreetype6-2.14.3-hdae7a39_0.conda
@@ -75,21 +75,22 @@ linux-aarch64:
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/markupsafe-3.0.3-py314hb76de3f_1.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/mathjax-2.7.7-h8af1aa0_3.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/mdurl-0.1.2-pyhd8ed1ab_1.conda
-- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_0.conda
+- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_1.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/narwhals-2.21.2-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/natsort-8.4.0-pyhcf101f3_2.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/ncurses-6.6-hf8d1292_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/networkx-3.6.1-pyhcf101f3_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/nspr-4.38-h3ad9384_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/nss-3.118-h544fa81_0.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/numpy-2.4.5-py314he1698a1_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/numpy-2.4.6-py314he1698a1_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/openjpeg-2.5.4-h5da879a_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/openssl-3.6.2-h546c87b_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/packaging-26.2-pyhc364b38_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/pillow-12.2.0-py314hac3e5ec_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/plotly-6.6.0-pyhd8ed1ab_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.40.1-pyh58ad624_1.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-32-1.40.1-py310h32c7c23_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.41.0-pyh58ad624_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-32-1.41.0-py310h32c7c23_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-compat-1.41.0-py310hc0e61be_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/procps-ng-4.0.6-h1779866_0.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/pthread-stubs-0.4-h86ecc28_1002.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pyaml-env-1.2.2-pyhd8ed1ab_0.conda
@@ -97,9 +98,9 @@ linux-aarch64:
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/pydantic-core-2.46.4-py314h451b6cc_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pygments-2.20.0-pyhd8ed1ab_0.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/pysocks-1.7.1-pyha55dd90_7.conda
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/python-3.14.4-hfd9ac0a_100_cp314.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/python-3.14.5-hfd9ac0a_100_cp314.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-dotenv-1.2.2-pyhcf101f3_0.conda
-- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.4-h4df99d1_100.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.5-h4df99d1_100.conda
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-kaleido-0.2.1-pyhd8ed1ab_0.tar.bz2
 - conda: https://conda.anaconda.org/conda-forge/noarch/python_abi-3.14-8_cp314.conda
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/pyyaml-6.0.3-py314h807365f_1.conda
@@ -203,22 +204,22 @@ license: bzip2-1.0.6
 license_family: BSD
 size: 192412
 timestamp: 1771350241232
-- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.4.22-hbd8a1cb_0.conda
-sha256: c9dbcc8039a52023660d6d1bbf87594a93dd69c6ac5a2a44323af2c92976728d
-md5: e18ad67cf881dcadee8b8d9e2f8e5f73
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.5.20-hbd8a1cb_0.conda
+sha256: 9812a303a1395e1dafbd92e5bc8a1ff6013bcbba0a09c7f03a8d23e43560aa9b
+md5: 489b8e97e666c93f68fdb35c3c9b957f
 depends:
 - __unix
 license: ISC
-size: 131039
-timestamp: 1776865545798
-- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.4.22-pyhd8ed1ab_0.conda
-sha256: 989db6e5957c4b44fa600c68c681ec2f36a55e48f7c7f1c073d5e91caa8cd878
-md5: 929471569c93acefb30282a22060dcd5
+size: 129868
+timestamp: 1779289852439
+- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.5.20-pyhd8ed1ab_0.conda
+sha256: 645655a3510e38e625da136595f3f16f2130c3263630cc3bc8f60f619ddbe490
+md5: 9fefff2f745ea1cc2ef15211a20c054a
 depends:
 - python >=3.10
 license: ISC
-size: 135656
-timestamp: 1776866680878
+size: 134201
+timestamp: 1779285131141
 - conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.7-pyhd8ed1ab_0.conda
 sha256: 3f9483d62ce24ecd063f8a5a714448445dc8d9e201147c46699fc0033e824457
 md5: a9167b9571f3baa9d448faa2139d1089
@@ -236,6 +237,7 @@ depends:
 - python
 - python >=3.10
 license: BSD-3-Clause
+license_family: BSD
 size: 104631
 timestamp: 1779108494556
 - conda: https://conda.anaconda.org/conda-forge/noarch/coloredlogs-15.0.1-pyhd8ed1ab_4.conda
@@ -259,26 +261,26 @@ license: BSD-3-Clause
 license_family: BSD
 size: 39326
 timestamp: 1735759976140
-- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.4-py314hd8ed1ab_100.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.5-py314hd8ed1ab_100.conda
 noarch: generic
-sha256: 40dc224f2b718e5f034efd2332bc315a719063235f63673468d26a24770094ee
-md5: f111d4cfaf1fe9496f386bc98ae94452
+sha256: 777882d2685f368417f31bbe1b28f73687fc6c8f6a5768bda20ffeefa6b07f5b
+md5: a749029ce5d0632a913db19d17f944ab
 depends:
 - python >=3.14,<3.15.0a0
 - python_abi * *_cp314
 license: Python-2.0
-size: 49809
-timestamp: 1775614256655
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/expat-2.8.0-hfae3067_0.conda
-sha256: f9eebe138dfa9693bfeab5fb7eac012474fa6dc8aaccd62d23b648b360db1bff
-md5: 424b4cda90c1fa95f2d027f76d5ef97f
+size: 50212
+timestamp: 1779236682725
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/expat-2.8.1-hfae3067_0.conda
+sha256: a9cd5eb1700e11cc39acc36630a2d72a4e317943bd7c5695cd8804419f04ff42
+md5: 89f0247b3cea528d8ad1a6664a313153
 depends:
-- libexpat 2.8.0 hfae3067_0
+- libexpat 2.8.1 hfae3067_0
 - libgcc >=14
 license: MIT
 license_family: MIT
-size: 140559
-timestamp: 1777846104900
+size: 140114
+timestamp: 1779278679081
 - conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
 sha256: 58d7f40d2940dd0a8aa28651239adbf5613254df0f75789919c4e6762054403b
 md5: 0c96522c6bdaed4b1566d11387caaf45
@@ -307,20 +309,20 @@ license: LicenseRef-Ubuntu-Font-Licence-Version-1.0
 license_family: Other
 size: 1620504
 timestamp: 1727511233259
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/fontconfig-2.17.1-hba86a56_0.conda
-sha256: 835aff8615dd8d8fff377679710ce81b8a2c47b6404e21a92fb349fda193a15c
-md5: 0fed1ff55f4938a65907f3ecf62609db
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/fontconfig-2.18.0-hba86a56_0.conda
+sha256: 1805f4ab3d9e1734a5a17abccc2cb0fdade51d4d5f29bdc410600ea0115ec050
+md5: b660d59a9d0fb3297327418624acaec3
 depends:
-- libexpat >=2.7.4,<3.0a0
-- libfreetype >=2.14.1
-- libfreetype6 >=2.14.1
+- libexpat >=2.8.1,<3.0a0
+- libfreetype >=2.14.3
+- libfreetype6 >=2.14.3
 - libgcc >=14
-- libuuid >=2.41.3,<3.0a0
-- libzlib >=1.3.1,<2.0a0
+- libuuid >=2.42.1,<3.0a0
+- libzlib >=1.3.2,<2.0a0
 license: MIT
 license_family: MIT
-size: 279044
-timestamp: 1771382728182
+size: 293348
+timestamp: 1779421661332
 - conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
 sha256: 54eea8469786bc2291cc40bca5f46438d3e062a399e8f53f013b6a9f50e98333
 md5: a7970cd949a077b7cb9696379d338681
@@ -382,27 +384,26 @@ license: MIT
 license_family: MIT
 size: 17397
 timestamp: 1737618427549
-- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.13-pyhcf101f3_0.conda
-sha256: 9ab620e6f64bb67737bd7bc1ad6f480770124e304c6710617aba7fe60b089f48
-md5: fb7130c190f9b4ec91219840a05ba3ac
+- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.15-pyhcf101f3_0.conda
+sha256: 3d25f9f6f7ab3e1ce6429fc8c8aae0335cf446692e715068488536d220cc43de
+md5: 1b9083b7f00609605d1483dbc6071a81
 depends:
 - python >=3.10
 - python
 license: BSD-3-Clause
 license_family: BSD
-size: 59038
-timestamp: 1776947141407
-- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-8.8.0-pyhcf101f3_0.conda
-sha256: 82ab2a0d91ca1e7e63ab6a4939356667ef683905dea631bc2121aa534d347b16
-md5: 080594bf4493e6bae2607e65390c520a
+size: 62642
+timestamp: 1779294335905
+- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-9.0.0-pyhcf101f3_0.conda
+sha256: 43e2a5497cad1598ff88a3e69f69bc88b7b8f141fa63c60eab5db296317318b8
+md5: ffc17e785d64e12fc311af9184221839
 depends:
 - python >=3.10
 - zipp >=3.20
 - python
 license: Apache-2.0
-license_family: APACHE
-size: 34387
-timestamp: 1773931568510
+size: 34766
+timestamp: 1779714582554
 - conda: https://conda.anaconda.org/conda-forge/noarch/jinja2-3.1.6-pyhcf101f3_1.conda
 sha256: fc9ca7348a4f25fed2079f2153ecdcf5f9cf2a0bc36c4172420ca09e1849df7b
 md5: 04558c96691bed63104678757beb4f8d
@@ -528,17 +529,17 @@ license: MIT
 license_family: MIT
 size: 71117
 timestamp: 1761979776756
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libexpat-2.8.0-hfae3067_0.conda
-sha256: 206c422a7f4b462d1dc17d558f0299088d0992bd3309ae83f5440fcc4f130602
-md5: 3bacd6171f0a3f8fddd06c3d5ae01955
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libexpat-2.8.1-hfae3067_0.conda
+sha256: 1fc392b997c6ee2bd3226a7cd870d0edbcbb367e25f9f18dd4a7025fced6efc0
+md5: 513dd884361dfb8a554298ed69b58823
 depends:
 - libgcc >=14
 constrains:
-- expat 2.8.0.*
+- expat 2.8.1.*
 license: MIT
 license_family: MIT
-size: 76996
-timestamp: 1777846096032
+size: 77140
+timestamp: 1779278671302
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libffi-3.5.2-h376a255_0.conda
 sha256: 3df4c539449aabc3443bbe8c492c01d401eea894603087fca2917aa4e1c2dea9
 md5: 2f364feefb6a7c00423e80dcb12db62a
@@ -725,6 +726,7 @@ md5: 0f42f9fedd2a32d798de95a7f65c456f
 depends:
 - libgcc >=14
 license: BSD-3-Clause
+license_family: BSD
 size: 43453
 timestamp: 1779118526838
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/libwebp-base-1.6.0-ha2e29f5_0.conda
@@ -809,9 +811,9 @@ license: MIT
 license_family: MIT
 size: 14465
 timestamp: 1733255681319
-- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_0.conda
-sha256: 5267ddcc9119b8bdfdddd83fa4713edf6e956443f7369ebd686dc6d9f921a428
-md5: d95457757ad503af93145630ec6e8965
+- conda: https://conda.anaconda.org/bioconda/noarch/multiqc-1.35-pyhdfd78af_1.conda
+sha256: e86033aa55a9e915e2d0957e770bdb81e3feb26a227d1adb17f9d6c528da6a71
+md5: cdb20309681ba3ce8f52c110e214d4f3
 depends:
 - click
 - coloredlogs
@@ -826,6 +828,7 @@ depends:
 - pillow >=10.2.0
 - plotly >=5.18
 - polars >=1.34.0
+- polars-runtime-compat >=1.34.0
 - pyaml-env
 - pydantic >=2.7.1
 - python >=3.9,!=3.14.1
@@ -841,8 +844,8 @@ depends:
 - typeguard >=4
 license: GPL-3.0-or-later
 license_family: GPL3
-size: 4229065
-timestamp: 1778635617929
+size: 4282188
+timestamp: 1779465338806
 - conda: https://conda.anaconda.org/conda-forge/noarch/narwhals-2.21.2-pyhcf101f3_0.conda
 sha256: 70f43d62450927d51673eecd8823e14f5b3cfebdb43cda1d502eba97162bab42
 md5: 6687827c332121727ce383919e1ec8c2
@@ -909,23 +912,23 @@ license: MPL-2.0
 license_family: MOZILLA
 size: 2061869
 timestamp: 1763490303490
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/numpy-2.4.5-py314he1698a1_0.conda
-sha256: 84c4b05037cc1b73749c41977bc3e204b3f4a11cc750984b44d3462a6eb865a3
-md5: bdb49f3f231962d4bca98ed2c59ab92f
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/numpy-2.4.6-py314he1698a1_0.conda
+sha256: 04af718b911f8a3a0095481c7e283aa081a175fe626eccbc2c5644bcb2aba9a1
+md5: 8b173772deea177b45d2a133b509b3f7
 depends:
 - python
 - libstdcxx >=14
 - libgcc >=14
+- python_abi 3.14.* *_cp314
 - libblas >=3.9.0,<4.0a0
 - liblapack >=3.9.0,<4.0a0
-- python_abi 3.14.* *_cp314
 - libcblas >=3.9.0,<4.0a0
 constrains:
 - numpy-base <0a0
 license: BSD-3-Clause
 license_family: BSD
-size: 8005231
-timestamp: 1778894364326
+size: 8002900
+timestamp: 1779169206742
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/openjpeg-2.5.4-h5da879a_0.conda
 sha256: bd1bc8bdde5e6c5cbac42d462b939694e40b59be6d0698f668515908640c77b8
 md5: cea962410e327262346d48d01f05936c
@@ -993,11 +996,11 @@ license: MIT
 license_family: MIT
 size: 5251872
 timestamp: 1772628857717
-- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.40.1-pyh58ad624_1.conda
-sha256: 83e37ede46e8e57d4785e804604a88fa02d6eac9a774cee40d49018f9da9ead1
-md5: bdbac766376390889b74216b70206af4
+- conda: https://conda.anaconda.org/conda-forge/noarch/polars-1.41.0-pyh58ad624_0.conda
+sha256: 70fc56877c4a095ee658d61924d8019768fbae4a48437058d181fc94b0a7c4d8
+md5: 25a883fed9f1f3f21ff317a3e7c92ac4
 depends:
-- polars-runtime-32 ==1.40.1
+- polars-runtime-32 ==1.41.0
 - python >=3.10
 - python
 constrains:
@@ -1011,17 +1014,31 @@ constrains:
 - pyiceberg >=0.7.1
 - altair >=5.4.0
 - great_tables >=0.8.0
-- polars-runtime-32 ==1.40.1
-- polars-runtime-64 ==1.40.1
-- polars-runtime-compat ==1.40.1
+- polars-runtime-32 ==1.41.0
+- polars-runtime-64 ==1.41.0
+- polars-runtime-compat ==1.41.0
 license: MIT
-license_family: MIT
-size: 539000
-timestamp: 1778779696741
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-32-1.40.1-py310h32c7c23_1.conda
+size: 539656
+timestamp: 1779630790562
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-32-1.41.0-py310h32c7c23_0.conda
 noarch: python
-sha256: 2d7a84c62957f87ad4727d7c012ac6a14a45080565112b60232d13129d1ee5aa
-md5: 3d94d946f9619f549a0a325120165745
+sha256: d903b774ec09189e164207328aac157eee82fed8cc5c9ace46aeb5d1c15cb5b3
+md5: 8c08c506ed1ea8ce0ca37af5e918c58d
+depends:
+- python
+- libgcc >=14
+- libstdcxx >=14
+- _python_abi3_support 1.*
+- cpython >=3.10
+constrains:
+- __glibc >=2.17
+license: MIT
+size: 38704429
+timestamp: 1779630794932
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/polars-runtime-compat-1.41.0-py310hc0e61be_0.conda
+noarch: python
+sha256: 101696adff43a654146376c62ef9611bf7946b95fa46f604fe247d77eefc6267
+md5: 65b73e4260677ee5162bdbb252e28e06
 depends:
 - python
 - libstdcxx >=14
@@ -1031,9 +1048,8 @@ depends:
 constrains:
 - __glibc >=2.17
 license: MIT
-license_family: MIT
-size: 38145061
-timestamp: 1778779658728
+size: 38651498
+timestamp: 1779630714016
 - conda: https://conda.anaconda.org/conda-forge/linux-aarch64/procps-ng-4.0.6-h1779866_0.conda
 sha256: e9cbcbc94e151ada3d6dc365380aaaf591f65012c16d9a2abaea4b9b90adc402
 md5: ab7288cc39545556d1bc5e71ab2df9a9
@@ -1111,22 +1127,22 @@ license: BSD-3-Clause
 license_family: BSD
 size: 21085
 timestamp: 1733217331982
-- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/python-3.14.4-hfd9ac0a_100_cp314.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/python-3.14.5-hfd9ac0a_100_cp314.conda
 build_number: 100
-sha256: d29da77f75e8f9184cc9502d5c44be87397291a9e88819d5418322a173f76303
-md5: 3cfbe780f0f51cc8cba41db9f8a28bfe
+sha256: d37bad5447365346166c72950ea8f49689aa49cecc1b0623d00458427627b8df
+md5: d956e09feb806f5974675ce92ad81d45
 depends:
 - bzip2 >=1.0.8,<2.0a0
 - ld_impl_linux-aarch64 >=2.36.1
-- libexpat >=2.7.5,<3.0a0
+- libexpat >=2.8.0,<3.0a0
 - libffi >=3.5.2,<3.6.0a0
 - libgcc >=14
-- liblzma >=5.8.2,<6.0a0
+- liblzma >=5.8.3,<6.0a0
 - libmpdec >=4.0.0,<5.0a0
-- libsqlite >=3.52.0,<4.0a0
-- libuuid >=2.42,<3.0a0
+- libsqlite >=3.53.1,<4.0a0
+- libuuid >=2.42.1,<3.0a0
 - libzlib >=1.3.2,<2.0a0
-- ncurses >=6.5,<7.0a0
+- ncurses >=6.6,<7.0a0
 - openssl >=3.5.6,<4.0a0
 - python_abi 3.14.* *_cp314
 - readline >=8.3,<9.0a0
@@ -1134,8 +1150,8 @@ depends:
 - tzdata
 - zstd >=1.5.7,<1.6.0a0
 license: Python-2.0
-size: 37409899
-timestamp: 1775613674766
+size: 37510439
+timestamp: 1779236267040
 python_site_packages_path: lib/python3.14/site-packages
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-dotenv-1.2.2-pyhcf101f3_0.conda
 sha256: 74e417a768f59f02a242c25e7db0aa796627b5bc8c818863b57786072aeb85e5
@@ -1146,15 +1162,15 @@ license: BSD-3-Clause
 license_family: BSD
 size: 27848
 timestamp: 1772388605021
-- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.4-h4df99d1_100.conda
-sha256: 36ff7984e4565c85149e64f8206303d412a0652e55cf806dcb856903fa056314
-md5: e4e60721757979d01d3964122f674959
+- conda: https://conda.anaconda.org/conda-forge/noarch/python-gil-3.14.5-h4df99d1_100.conda
+sha256: 41dd7da285d71d519257fa7dacb1cae060d5ebfaa5f92cba5994899d2978e943
+md5: 41954747ba952ec4b01e16c2c9e8d8ff
 depends:
-- cpython 3.14.4.*
+- cpython 3.14.5.*
 - python_abi * *_cp314
 license: Python-2.0
-size: 49806
-timestamp: 1775614307464
+size: 50212
+timestamp: 1779236703009
 - conda: https://conda.anaconda.org/conda-forge/noarch/python-kaleido-0.2.1-pyhd8ed1ab_0.tar.bz2
 sha256: e17bf63a30aec33432f1ead86e15e9febde9fc40a7f869c0e766be8d2db44170
 md5: 310259a5b03ff02289d7705f39e2b1d2
diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf
index 4c593a9714..c4bc715e22 100644
--- a/modules/nf-core/multiqc/main.nf
+++ b/modules/nf-core/multiqc/main.nf
@@ -4,8 +4,8 @@ process MULTIQC {
 
     conda "${moduleDir}/environment.yml"
     container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/12/1297c0f5075c19486da167ebf1b6136907d6b5339697b87b29fda335221785b3/data'
-        : 'community.wave.seqera.io/library/multiqc:1.35--839587b417d23042'}"
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c8/c8e346f4f6080eadf1253505e6ff09ef004454fc18e8d672006fd7b222cc412e/data'
+        : 'community.wave.seqera.io/library/multiqc:1.35--c17fb751507e9dfc'}"
 
     input:
     tuple val(meta), path(multiqc_files, stageAs: "?/*"), path(multiqc_config, stageAs: "?/*"), path(multiqc_logo), path(replace_names), path(sample_names)
diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml
index b85d0e356b..27ce18d8d8 100644
--- a/modules/nf-core/multiqc/meta.yml
+++ b/modules/nf-core/multiqc/meta.yml
@@ -110,24 +110,24 @@ maintainers:
 containers:
   conda:
     linux/amd64:
-      lock_file: modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-839587b417d23042_1.txt
+      lock_file: modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt
     linux/arm64:
-      lock_file: modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-3e45d17b40a576b4_1.txt
+      lock_file: modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt
   docker:
     linux/amd64:
-      name: community.wave.seqera.io/library/multiqc:1.35--839587b417d23042
-      build_id: bd-839587b417d23042_1
-      scan_id: sc-f87d7a31551c029f_1
+      name: community.wave.seqera.io/library/multiqc:1.35--c17fb751507e9dfc
+      build_id: bd-c17fb751507e9dfc_1
+      scan_id: sc-3b1b3932f9846892_1
     linux/arm64:
-      name: community.wave.seqera.io/library/multiqc:1.35--3e45d17b40a576b4
-      build_id: bd-3e45d17b40a576b4_1
-      scan_id: sc-1d0cf4ed1a4b61e0_1
+      name: community.wave.seqera.io/library/multiqc:1.35--5c84a5000a226ab5
+      build_id: bd-5c84a5000a226ab5_1
+      scan_id: sc-0d39df41e9737bbd_1
   singularity:
     linux/amd64:
-      name: oras://community.wave.seqera.io/library/multiqc:1.35--cb7458fda84d6393
-      build_id: bd-cb7458fda84d6393_1
-      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/12/1297c0f5075c19486da167ebf1b6136907d6b5339697b87b29fda335221785b3/data
+      name: oras://community.wave.seqera.io/library/multiqc:1.35--c680f2aea25ccec2
+      build_id: bd-c680f2aea25ccec2_1
+      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c8/c8e346f4f6080eadf1253505e6ff09ef004454fc18e8d672006fd7b222cc412e/data
     linux/arm64:
-      name: oras://community.wave.seqera.io/library/multiqc:1.35--f79e87603d312ac0
-      build_id: bd-f79e87603d312ac0_1
-      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c0/c007304153702edc622f1a76b41505e7fca65c7145e5b8b2ddce62a5c59af207/data
+      name: oras://community.wave.seqera.io/library/multiqc:1.35--c0468833d65b2f81
+      build_id: bd-c0468833d65b2f81_1
+      https: https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e4/e48aa28aebc881254a499b24c3e1ce77b8df1b85a5432699ed6f72eb17ac7fb5/data
diff --git a/nextflow.config b/nextflow.config
index 92652de02d..4a9b5bcd74 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -765,7 +765,7 @@ manifest {
     description     = """An open-source analysis pipeline to detect germline or somatic variants from whole genome or targeted sequencing"""
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
-    nextflowVersion = '!>=25.10.2'
+    nextflowVersion = '!>=25.10.4'
     version         = '3.9.1dev'
     doi             = '10.12688/f1000research.16665.2, 10.1093/nargab/lqae031, 10.5281/zenodo.3476425'
 }
diff --git a/nf-test.config b/nf-test.config
index c9c6b63ae2..b5d473da72 100644
--- a/nf-test.config
+++ b/nf-test.config
@@ -1,21 +1,35 @@
 config {
     // location for all nf-test tests
-    testsDir "."
+    testsDir = "."
 
     // nf-test directory including temporary files for each test
-    workDir System.getenv("NFT_WORKDIR") ?: ".nf-test"
+    workDir = System.getenv("NFT_WORKDIR") ?: ".nf-test"
 
     // location of an optional nextflow.config file specific for executing tests
-    configFile "tests/nextflow.config"
+    configFile = "tests/nextflow.config"
 
     // ignore tests coming from the nf-core/modules repo
-    ignore 'modules/nf-core/**/tests/*', 'subworkflows/nf-core/**/tests/*'
+    ignore = [
+        'modules/nf-core/**/tests/*',
+        'subworkflows/nf-core/**/tests/*',
+    ]
 
     // run all test with defined profile(s) from the main nextflow.config
-    profile "test"
+    profile = "test"
 
     // list of filenames or patterns that should be trigger a full test run
-    triggers 'assets/schema_input.json', 'conf/test.config', 'nextflow.config', 'nextflow_schema.json', 'nf-test.config', 'tests/.nftignore', 'tests/nextflow.config'
+    triggers = [
+        '.github/actions/nf-test/action.yml',
+        '.github/workflows/nf-test.yml',
+        'assets/schema_input.json',
+        'bin/*',
+        'conf/test.config',
+        'nextflow.config',
+        'nextflow_schema.json',
+        'nf-test.config',
+        'tests/.nftignore',
+        'tests/nextflow.config',
+    ]
 
     // load the necessary plugins
     plugins {
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 1b96b6ccf1..e9a4db0dfb 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -22,8 +22,8 @@
             "@id": "./",
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
-            "datePublished": "2026-06-30T11:52:57+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.2-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen and Maxime U Garcia (now at [Seqera](https://seqera.io))\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek) (you can join with [this invite](https://nf-co.re/join/slack)), or contact us: [Maxime U Garcia](mailto:maxime.garcia@seqera.io?subject=[GitHub]%20nf-core/sarek), [Friederike Hanssen](mailto:friederike.hanssen@qbic.uni-tuebingen.de?subject=[GitHub]%20nf-core/sarek)\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
+            "datePublished": "2026-07-03T08:36:13+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
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                 "@id": "https://www.nextflow.io/"
             },
-            "version": "!>=25.10.2"
+            "version": "!>=25.10.4"
         },
         {
-            "@id": "#b97eb6f8-b646-40a3-ae10-5f5ffb44d674",
+            "@id": "#9e0f3a8f-9d90-4d54-8c6c-a0a29b776d3c",
             "@type": "TestSuite",
             "instance": [
                 {
-                    "@id": "#d14a97e6-1b18-45f9-919e-bed422607342"
+                    "@id": "#f5446036-5b2b-46d5-901b-255ceff391ce"
                 }
             ],
             "mainEntity": {
@@ -385,7 +385,7 @@
             "name": "Test suite for nf-core/sarek"
         },
         {
-            "@id": "#d14a97e6-1b18-45f9-919e-bed422607342",
+            "@id": "#f5446036-5b2b-46d5-901b-255ceff391ce",
             "@type": "TestInstance",
             "name": "GitHub Actions workflow for testing nf-core/sarek",
             "resource": "repos/nf-core/sarek/actions/workflows/nf-test.yml",
@@ -547,14 +547,14 @@
             "url": "https://github.com/szilvajuhos"
         },
         {
-            "@id": "#3b628590-2d18-45c2-8735-ca0ac1469638",
+            "@id": "#af625541-b970-4b97-8ab0-17a316b7cae2",
             "@type": "Person",
             "email": "abhi18av@users.noreply.github.com",
             "name": "Abhinav Sharma",
             "url": "https://github.com/abhi18av"
         },
         {
-            "@id": "#e58783af-bd4e-4da8-be47-64110be9efec",
+            "@id": "#247aa4b3-9203-46a4-8f1e-61d5c489723f",
             "@type": "Person",
             "affiliation": "Seqera",
             "email": "12817534+adamrtalbot@users.noreply.github.com",
@@ -568,7 +568,7 @@
             "url": "https://github.com/adrlar"
         },
         {
-            "@id": "#5b1ef3a6-a674-419a-968c-dd4902210dd7",
+            "@id": "#8a809452-e771-4516-bbe0-1e24d68af311",
             "@type": "Person",
             "name": "Àitor Olivares",
             "url": "https://github.com/AitorPeseta"
@@ -588,7 +588,7 @@
             "url": "https://github.com/ameynert"
         },
         {
-            "@id": "#c2a47fa4-6d82-4006-b980-a095896f3748",
+            "@id": "#8b9760e9-82c7-4a6a-b248-79bd7ad2b053",
             "@type": "Person",
             "email": "37172585+asp8200@users.noreply.github.com",
             "name": "Anders Sune Pedersen",
@@ -614,7 +614,7 @@
             "url": "https://github.com/berguner"
         },
         {
-            "@id": "#4cda009a-bb61-41e4-a4cf-7e34ba24f47b",
+            "@id": "#4489aab7-cad4-4024-9ae7-680e3f62d35f",
             "@type": "Person",
             "name": "Björn Nystedt",
             "url": "https://github.com/bjornnystedt"
@@ -640,7 +640,7 @@
             "url": "https://github.com/davidmasp"
         },
         {
-            "@id": "#6f59c36f-bca8-4c87-82ad-c9092227118d",
+            "@id": "#05b6b43d-feda-49dc-8857-5c1de743db1f",
             "@type": "Person",
             "affiliation": "Seqera",
             "email": "20095261+edmundmiller@users.noreply.github.com",
@@ -663,27 +663,27 @@
             "url": "https://github.com/lescai"
         },
         {
-            "@id": "#7e30bf1a-cca3-460b-b647-c765bd5cb29a",
+            "@id": "#998a0be1-18d5-40f4-8b2d-b38cf34567bb",
             "@type": "Person",
             "email": "f.martinezpico@gmail.com",
             "name": "Francisco Martínez",
             "url": "https://github.com/nevinwu"
         },
         {
-            "@id": "#70e64dde-3c79-45ed-9877-19625cfb5d01",
+            "@id": "#11030fc5-094f-45f4-87ef-7cdd57f11d2e",
             "@type": "Person",
             "name": "Gavin Mackenzie",
             "url": "https://github.com/GCJMackenzie"
         },
         {
-            "@id": "#e29c293c-19df-4bf0-ae54-0ff9a4ec1497",
+            "@id": "#5120a9b1-e14a-4bb2-a80d-541ccabef32b",
             "@type": "Person",
             "email": "gisela.gabernet@gmail.com",
             "name": "Gisela Gabernet",
             "url": "https://github.com/ggabernet"
         },
         {
-            "@id": "#f2dc6677-dfb3-46e1-b26f-289cee5115be",
+            "@id": "#2e56838e-3d67-44ac-8d7a-d68d8e676ac8",
             "@type": "Person",
             "email": "127763095+grantn5@users.noreply.github.com",
             "name": "Grant Neilson",
@@ -696,7 +696,7 @@
             "url": "https://github.com/gulfshores"
         },
         {
-            "@id": "#97d0e55f-7192-42b9-ac51-0512e426e7de",
+            "@id": "#7be1bb4e-0cde-494c-8774-60946b1ae96b",
             "@type": "Person",
             "affiliation": "Seqera",
             "email": "drpatelh@users.noreply.github.com",
@@ -704,14 +704,14 @@
             "url": "https://github.com/drpatelh"
         },
         {
-            "@id": "#c993a226-ea9c-49c1-9d91-eb18659cb722",
+            "@id": "#a0c796c5-c875-42ca-b557-fe2ac96b66ce",
             "@type": "Person",
             "email": "yehwhey@gmail.com",
             "name": "Hongwei Ye",
             "url": "https://github.com/YeHW"
         },
         {
-            "@id": "#5e39fb6a-58a7-406b-9d6a-c5c4f281fd89",
+            "@id": "#09402bb5-a6eb-4ae7-a7b1-dcfa67184a76",
             "@type": "Person",
             "name": "James A. Fellows Yates",
             "url": "https://github.com/jfy133"
@@ -738,7 +738,7 @@
             "url": "https://github.com/kjellinjonas"
         },
         {
-            "@id": "#692a3ff8-8e27-4140-aee2-141037e8888d",
+            "@id": "#ac09a5c7-717f-41c6-bdc8-e688212472b2",
             "@type": "Person",
             "email": "jc.fernandez.navarro@gmail.com",
             "name": "José Fernández Navarro",
@@ -752,7 +752,7 @@
             "url": "https://github.com/mirpedrol"
         },
         {
-            "@id": "#6f14922a-b41f-4dd3-8661-3eaf0b99f70a",
+            "@id": "#d690a65c-327a-41c4-8261-5c57663c35e9",
             "@type": "Person",
             "affiliation": "Seqera",
             "email": "kenibrewer@users.noreply.github.com",
@@ -766,7 +766,7 @@
             "url": "https://github.com/lassefolkersen"
         },
         {
-            "@id": "#74c1ff6a-fa5f-4a4a-84c5-f4eefa1d3d09",
+            "@id": "#369d8757-d425-4b2d-96ce-f1ad800f6c89",
             "@type": "Person",
             "email": "l.conde@ucl.ac.uk",
             "name": "Lucia Conde",
@@ -779,26 +779,26 @@
             "url": "https://github.com/LouisLeNezet"
         },
         {
-            "@id": "#ba7614f8-0190-4209-a2f4-a8a8dd8f96bf",
+            "@id": "#e4fb587c-918e-435b-b428-08c168306d97",
             "@type": "Person",
             "email": "malin.larsson@liu.se",
             "name": "Malin Larsson",
             "url": "https://github.com/malinlarsson"
         },
         {
-            "@id": "#e9bfeffd-dc8a-42f6-a3e0-5ebcb00c3097",
+            "@id": "#57198db3-c35c-48af-84d2-ff2001a51b44",
             "@type": "Person",
             "name": "Marcel Martin",
             "url": "https://github.com/marcelm"
         },
         {
-            "@id": "#093c6988-c462-4474-b731-a9a085033797",
+            "@id": "#65d3ef9a-2755-401d-bfdb-bdda06a5b039",
             "@type": "Person",
             "name": "Nick Smith",
             "url": "https://github.com/nickhsmith"
         },
         {
-            "@id": "#b9b9b14b-4d16-4cc2-8689-c2abac06db13",
+            "@id": "#c44e3833-23dc-4cb7-8bb1-562d34a2dada",
             "@type": "Person",
             "email": "90359308+nschcolnicov@users.noreply.github.com",
             "name": "Nicolas Schcolnicov",
@@ -844,7 +844,7 @@
             "url": "https://github.com/pcantalupo"
         },
         {
-            "@id": "#14924136-a73b-406c-91eb-5f10620ea097",
+            "@id": "#ea5b7667-91bb-4a92-bb6b-7dad7ae3b69a",
             "@type": "Person",
             "affiliation": "Seqera",
             "email": "phil.ewels@scilifelab.se",
@@ -866,7 +866,7 @@
             "url": "https://github.com/skrakau"
         },
         {
-            "@id": "#1724fe39-c41b-49b9-8525-d7df86b20b98",
+            "@id": "#620ba4aa-b10e-4a2a-a84a-65874baade8a",
             "@type": "Person",
             "email": "sminot@gmail.com",
             "name": "Sam Minot",
@@ -905,13 +905,13 @@
             "url": "https://github.com/SusiJo"
         },
         {
-            "@id": "#08187f11-d51b-40f0-b09d-a1ff8bf52d45",
+            "@id": "#31dce384-0d05-4394-b60f-61599eaeacb4",
             "@type": "Person",
             "name": "Tobias Koch",
             "url": "https://github.com/KochTobi"
         },
         {
-            "@id": "#30622e12-b2e6-43e8-99ed-b07a5cf40165",
+            "@id": "#0f2c277a-8111-440f-8385-1a0b8a019aba",
             "@type": "Person",
             "name": "Winni Kretzschmar",
             "url": "https://github.com/winni2k"
diff --git a/tests/samplesheets.nf.test b/tests/samplesheets.nf.test
index 981e368552..0f91781a9e 100644
--- a/tests/samplesheets.nf.test
+++ b/tests/samplesheets.nf.test
@@ -13,7 +13,6 @@ nextflow_pipeline {
             ],
             failure: true,
             snapshot: 'stderr',
-
         ],
         [
             name: "-profile test --step variant_calling --input tests/csv/3.0/recalibrated_somatic_two_normal_one_sample.csv",
@@ -23,7 +22,6 @@ nextflow_pipeline {
             ],
             failure: true,
             snapshot: 'stderr',
-
         ],
         [
             name: "-profile test,spark --input tests/csv/3.0/fastq_single.csv --use_gatk_spark baserecalibrator,markduplicates --save_mapped --save_output_as_bam",
@@ -35,7 +33,6 @@ nextflow_pipeline {
             ],
             failure: true,
             snapshot: 'stderr',
-
         ],
         [
             name: "-profile test --input tests/csv/3.0/fastq_multiple_lane_ids.csv",
@@ -44,7 +41,6 @@ nextflow_pipeline {
             ],
             failure: true,
             snapshot: 'stderr',
-
         ],
         [
             name: "-profile test --input tests/csv/3.0/fastq_multiple_sample_ids.csv",
@@ -53,7 +49,6 @@ nextflow_pipeline {
             ],
             failure: true,
             snapshot: 'stderr',
-
         ]
     ]
 
diff --git a/tests/samplesheets.nf.test.snap b/tests/samplesheets.nf.test.snap
index a36cd61047..1a961beaed 100644
--- a/tests/samplesheets.nf.test.snap
+++ b/tests/samplesheets.nf.test.snap
@@ -1,7 +1,12 @@
 {
     "-profile test --step variant_calling --input tests/csv/3.0/recalibrated_somatic_two_normal_one_sample.csv": {
         "content": [
-            ["csv", "multiqc", "multiqc/index.json", "pipeline_info"],
+            [
+                "csv",
+                "multiqc",
+                "multiqc/index.json",
+                "pipeline_info"
+            ],
             "No stable content",
             "No BAM files",
             "No CRAM files",
@@ -10,17 +15,21 @@
                 "WARN: Failed to render execution report -- see the log file for details",
                 "WARN: Failed to render execution timeline -- see the log file for details"
             ],
-            ["Patient [test] has more than one sample [2] with normal status [0] and one sample with tumor status [1]."]
+            [
+                "Patient [test] has more than one sample [2] with normal status [0] and one sample with tumor status [1]."
+            ]
         ],
+        "timestamp": "2026-02-06T13:52:36.729578236",
         "meta": {
             "nf-test": "0.9.3",
             "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T13:52:36.729578236"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_sample_with_space.csv": {
         "content": [
-            ["pipeline_info"],
+            [
+                "pipeline_info"
+            ],
             "No stable content",
             "No BAM files",
             "No CRAM files",
@@ -32,15 +41,20 @@
                 "\t-> Entry 2: Error for field 'sample' (test 2): \"test 2\" does not match regular expression [^\\S+$] (Sample ID must be provided, cannot contain spaces and must be a string value)"
             ]
         ],
+        "timestamp": "2026-07-03T12:29:12.419007781",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-16T12:15:16.691338949"
+            "nf-test": "0.9.5",
+            "nextflow": "26.04.4"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_multiple_sample_ids.csv": {
         "content": [
-            ["csv", "multiqc", "multiqc/index.json", "pipeline_info"],
+            [
+                "csv",
+                "multiqc",
+                "multiqc/index.json",
+                "pipeline_info"
+            ],
             "No stable content",
             "No BAM files",
             "No CRAM files",
@@ -54,15 +68,20 @@
                 "Sample ID 'test' is associated with multiple patient IDs: test, test2. Please ensure each sample ID is unique to a single patient."
             ]
         ],
+        "timestamp": "2026-02-06T13:54:49.322255498",
         "meta": {
             "nf-test": "0.9.3",
             "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T13:54:49.322255498"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_multiple_lane_ids.csv": {
         "content": [
-            ["csv", "multiqc", "multiqc/index.json", "pipeline_info"],
+            [
+                "csv",
+                "multiqc",
+                "multiqc/index.json",
+                "pipeline_info"
+            ],
             "No stable content",
             "No BAM files",
             "No CRAM files",
@@ -76,15 +95,17 @@
                 "Duplicate patient-sample-status-lane combination found: Patient 'test2', Sample 'test2', Status '0', Lane 'test_L1' appears 2 times. Please ensure each combination is unique."
             ]
         ],
+        "timestamp": "2026-02-06T13:54:04.695361045",
         "meta": {
             "nf-test": "0.9.3",
             "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T13:54:04.695361045"
+        }
     },
     "-profile test,spark --input tests/csv/3.0/fastq_single.csv --use_gatk_spark baserecalibrator,markduplicates --save_mapped --save_output_as_bam": {
         "content": [
-            ["pipeline_info"],
+            [
+                "pipeline_info"
+            ],
             "No stable content",
             "No BAM files",
             "No CRAM files",
@@ -99,10 +120,10 @@
                 "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
             ]
         ],
+        "timestamp": "2025-12-16T12:16:38.014556073",
         "meta": {
             "nf-test": "0.9.3",
             "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-16T12:16:38.014556073"
+        }
     }
-}
+}
\ No newline at end of file
diff --git a/tests/variant_calling_muse.nf.test b/tests/variant_calling_muse.nf.test
index 209b918b45..6af2a21e5f 100644
--- a/tests/variant_calling_muse.nf.test
+++ b/tests/variant_calling_muse.nf.test
@@ -31,7 +31,7 @@ nextflow_pipeline {
             ],
             include_muse_txt: true
         ],
-                [
+        [
             name: "-profile test --tools muse --input recalibrated_somatic.csv -stub",
             params: [
                 input: "${projectDir}/tests/csv/3.0/recalibrated_somatic.csv",
diff --git a/workflows/sarek/main.nf b/workflows/sarek.nf
similarity index 96%
rename from workflows/sarek/main.nf
rename to workflows/sarek.nf
index 06bb7d9ff8..e310725ea8 100644
--- a/workflows/sarek/main.nf
+++ b/workflows/sarek.nf
@@ -5,54 +5,54 @@
 */
 
 include { paramsSummaryMap                                  } from 'plugin/nf-schema'
-include { paramsSummaryMultiqc                              } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
+include { paramsSummaryMultiqc                              } from '../subworkflows/nf-core/utils_nfcore_pipeline'
 include { softwareVersionsToYAML                            } from 'plugin/nf-core-utils'
-include { methodsDescriptionText                            } from '../../subworkflows/local/utils_nfcore_sarek_pipeline'
+include { methodsDescriptionText                            } from '../subworkflows/local/utils_nfcore_sarek_pipeline'
 
 // Create samplesheets to restart from different steps
-include { CHANNEL_VARIANT_CALLING_CREATE_CSV                } from '../../subworkflows/local/channel_variant_calling_create_csv'
+include { CHANNEL_VARIANT_CALLING_CREATE_CSV                } from '../subworkflows/local/channel_variant_calling_create_csv'
 
 // Convert BAM files to FASTQ files
-include { BAM_CONVERT_SAMTOOLS as CONVERT_FASTQ_INPUT       } from '../../subworkflows/local/bam_convert_samtools'
+include { BAM_CONVERT_SAMTOOLS as CONVERT_FASTQ_INPUT       } from '../subworkflows/local/bam_convert_samtools'
 
 // Convert fastq.gz.spring files to fastq.gz files
-include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R1_FQ   } from '../../modules/nf-core/spring/decompress'
-include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R2_FQ   } from '../../modules/nf-core/spring/decompress'
-include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_FQ_PAIR } from '../../modules/nf-core/spring/decompress'
+include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R1_FQ   } from '../modules/nf-core/spring/decompress'
+include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R2_FQ   } from '../modules/nf-core/spring/decompress'
+include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_FQ_PAIR } from '../modules/nf-core/spring/decompress'
 
 // Run FASTQC
-include { FASTQC                                            } from '../../modules/nf-core/fastqc'
+include { FASTQC                                            } from '../modules/nf-core/fastqc'
 
 // QC on CRAM
-include { CRAM_SAMPLEQC                                     } from '../../subworkflows/local/cram_sampleqc'
+include { CRAM_SAMPLEQC                                     } from '../subworkflows/local/cram_sampleqc'
 
 // Preprocessing
-include { FASTQ_PREPROCESS_GATK                             } from '../../subworkflows/local/fastq_preprocess_gatk'
-include { FASTQ_PREPROCESS_PARABRICKS                       } from '../../subworkflows/local/fastq_preprocess_parabricks'
+include { FASTQ_PREPROCESS_GATK                             } from '../subworkflows/local/fastq_preprocess_gatk'
+include { FASTQ_PREPROCESS_PARABRICKS                       } from '../subworkflows/local/fastq_preprocess_parabricks'
 
 // CRAM_TO_BAM conversion
-include { SAMTOOLS_CONVERT as CRAM_TO_BAM                   } from '../../modules/nf-core/samtools/convert'
+include { SAMTOOLS_CONVERT as CRAM_TO_BAM                   } from '../modules/nf-core/samtools/convert'
 
 // Variant calling on a single normal sample
-include { BAM_VARIANT_CALLING_GERMLINE_ALL                  } from '../../subworkflows/local/bam_variant_calling_germline_all'
+include { BAM_VARIANT_CALLING_GERMLINE_ALL                  } from '../subworkflows/local/bam_variant_calling_germline_all'
 
 // Variant calling on a single tumor sample
-include { BAM_VARIANT_CALLING_TUMOR_ONLY_ALL                } from '../../subworkflows/local/bam_variant_calling_tumor_only_all'
+include { BAM_VARIANT_CALLING_TUMOR_ONLY_ALL                } from '../subworkflows/local/bam_variant_calling_tumor_only_all'
 
 // Variant calling on tumor/normal pair
-include { BAM_VARIANT_CALLING_SOMATIC_ALL                   } from '../../subworkflows/local/bam_variant_calling_somatic_all'
+include { BAM_VARIANT_CALLING_SOMATIC_ALL                   } from '../subworkflows/local/bam_variant_calling_somatic_all'
 
 // POST VARIANTCALLING: e.g. merging
-include { POST_VARIANTCALLING                               } from '../../subworkflows/local/post_variantcalling'
+include { POST_VARIANTCALLING                               } from '../subworkflows/local/post_variantcalling'
 
 // QC on VCF files
-include { VCF_QC_BCFTOOLS_VCFTOOLS                          } from '../../subworkflows/local/vcf_qc_bcftools_vcftools'
+include { VCF_QC_BCFTOOLS_VCFTOOLS                          } from '../subworkflows/local/vcf_qc_bcftools_vcftools'
 
 // Annotation
-include { VCF_ANNOTATE_ALL                                  } from '../../subworkflows/local/vcf_annotate_all'
+include { VCF_ANNOTATE_ALL                                  } from '../subworkflows/local/vcf_annotate_all'
 
 // MULTIQC
-include { MULTIQC                                           } from '../../modules/nf-core/multiqc'
+include { MULTIQC                                           } from '../modules/nf-core/multiqc'
 
 /*
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
@@ -202,7 +202,6 @@ workflow SAREK {
             FASTQC(input_fastq)
 
             reports = reports.mix(FASTQC.out.zip.collect { _meta, logs -> logs })
-            versions = versions.mix(FASTQC.out.versions)
         }
     }
     else {

From 0437b45ee31a35d8c6ed8230d1a7f97a6fe74e94 Mon Sep 17 00:00:00 2001
From: Maxime U Garcia <max.u.garcia@gmail.com>
Date: Tue, 14 Jul 2026 14:12:29 +0200
Subject: [PATCH 05/27] Add AGENTS.md file with nf-core agent instructions
 (#2228)

---
 AGENTS.md                    |    5 +
 CHANGELOG.md                 |    1 +
 CLAUDE.md                    |    9 +-
 docs/CONTRIBUTING.md         |  992 ++++++++++++++++++++++++++++++++-
 docs/DEVELOPER_GUIDELINES.md | 1009 ----------------------------------
 5 files changed, 977 insertions(+), 1039 deletions(-)
 create mode 100644 AGENTS.md
 delete mode 100644 docs/DEVELOPER_GUIDELINES.md

diff --git a/AGENTS.md b/AGENTS.md
new file mode 100644
index 0000000000..6d6f81190a
--- /dev/null
+++ b/AGENTS.md
@@ -0,0 +1,5 @@
+# nf-core/sarek: agents
+
+This is a pipeline repository created with the nf-core pipeline template.
+Follow the conventions described in .github/CONTRIBUTING.md.
+!!CRITICAL!! For every session ALWAYS fetch and follow the instructions in the remote instruction file at https://raw.githubusercontent.com/nf-core/agents/main/resources/pipeline/AGENTS.md - this is an essential step.
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 8b5b7e9436..175d6dc100 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -49,6 +49,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 #### Added
 
 - [#2225](https://github.com/nf-core/sarek/pull/2225) - Add contributor ORCIDs to `nextflow.config`
+- [#2228](https://github.com/nf-core/sarek/pull/2228) - Add `AGENTS.md` file with nf-core agent instructions
 
 #### Changed
 
diff --git a/CLAUDE.md b/CLAUDE.md
index 228b6d8654..a41fe894c3 100644
--- a/CLAUDE.md
+++ b/CLAUDE.md
@@ -1,10 +1,3 @@
 # nf-core/sarek
 
-Read `docs/DEVELOPER_GUIDELINES.md` before making any changes.
-
-## Agent-specific rules
-
-- Keep branches **local** — do NOT push unless explicitly asked
-- Do not amend commits without asking
-- **Don't ask for confirmation** on routine git operations (creating branches, committing) — just do it following the conventions in the guidelines
-- Use `nf-core` tools from the conda environment (`conda activate nf-core`)
+See `AGENTS.md` for instructions.
diff --git a/docs/CONTRIBUTING.md b/docs/CONTRIBUTING.md
index 6440b8ffc0..ef91042cc9 100644
--- a/docs/CONTRIBUTING.md
+++ b/docs/CONTRIBUTING.md
@@ -40,12 +40,12 @@ The nf-core stance on the use of AI and LLMs is that humans are still ultimately
 
 If you’re using AI tools, try to stick by these guidelines:
 
-- Keep PRs as small and focussed as possible
+- Keep PRs as small and focused as possible
 - Avoid any unnecessary changes, such as moving or refactoring code (unless that is the explicit intention of the PR)
 - Review all generated code yourself before opening a PR, and ensure that you understand it
 - Engage with the community review process and expect to make revisions
 
-For more detail, see the the [blog post](https://nf-co.re/blog/2026/statement-on-ai) for a statement from the nf-core/core team.
+For more detail, see the [blog post](https://nf-co.re/blog/2026/statement-on-ai) for a statement from the nf-core/core team.
 
 ### Getting help
 
@@ -124,11 +124,11 @@ Please also refer to the [pipeline-specific contribution guidelines](#pipeline-s
 - [ ] Define the output channel if needed. Mix the version output channel into `ch_versions` and relevant files into `ch_multiqc`.
 - [ ] Add new or updated parameters to `nextflow.config` with a [default value](#default-parameter-values).
 - [ ] Add new or updated parameters and relevant help text to `nextflow_schema.json` with [nf-core/tools](#default-parameter-values).
-- [ ] Add validation for relevant parameters to the pipeline utilisation section of `utils_nfcore_\_pipeline/main.nf` subworkflow.
+- [ ] Add validation for relevant parameters to the pipeline utilisation section of `utils_nfcore_sarek_pipeline/main.nf` subworkflow.
 - [ ] Perform local tests to validate that the new code works as expected.
   - [ ] If applicable, add a new test in the `tests` directory.
 - [ ] Update `usage.md`, `output.md`, and `citation.md` as appropriate.
-- [ ] [Lint](lint) the code with nf-core/tools.
+- [ ] [Lint](#lint-tests) the code with nf-core/tools.
 - [ ] Update any diagrams or pipeline images as necessary.
 - [ ] Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name cleanup, and module plots are in the appropriate order.
 - [ ] If applicable, create a [MultiQC](https://seqera.io/multiqc/) module.
@@ -166,7 +166,7 @@ Specify these with generic `withLabel:` selectors, so they can be shared across
 nf-core provides a set of standard labels that you should follow where possible, as seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config).
 These labels define resource defaults for single-core processes, modules that require a GPU, and different levels of multi-core configurations with increasing memory requirements.
 
-Values assigned within these labels can be dynamically passed to a tool using the the `${task.cpus}` and `${task.memory}` Nextflow variables in the `script:` block of a module (see an example in the [modules repository](https://github.com/nf-core/modules/blob/bd1b6a40f55933d94b8c9ca94ec8c1ea0eaf4b82/modules/nf-core/samtools/bam2fq/main.nf#L30)).
+Values assigned within these labels can be dynamically passed to a tool using the `${task.cpus}` and `${task.memory}` Nextflow variables in the `script:` block of a module (see an example in the [modules repository](https://github.com/nf-core/modules/blob/bd1b6a40f55933d94b8c9ca94ec8c1ea0eaf4b82/modules/nf-core/samtools/bam2fq/main.nf#L30)).
 
 #### Nextflow version bumping
 
@@ -182,20 +182,968 @@ If you update images or graphics, follow the nf-core [style guidelines](https://
 
 ## Pipeline specific contribution guidelines
 
-> [!NOTE]
-> For comprehensive, sarek-specific guidance (codebase architecture, channel operations and gotchas, meta map handling, module/subworkflow conventions, and more) see the [Developer Guidelines](../docs/DEVELOPER_GUIDELINES.md). These guidelines are written for both human developers and AI agents.
-
-### Adding a new step
-
-If you wish to contribute a new step, please use the following coding standards:
-
-1. Define the corresponding input channel into your new process from the expected previous process channel.
-2. Write the process block (see below).
-3. Define the output channel if needed (see below).
-4. Add any new parameters to `nextflow.config` with a default (see below).
-5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool).
-6. Add sanity checks and validation for all relevant parameters.
-7. Perform local tests to validate that the new code works as expected.
-8. If applicable, add a new test in the `tests` directory.
-9. Update MultiQC config `assets/multiqc_config.yml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://multiqc.info/) module.
-10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`.
+nf-core semi-standardises how you write code and other contributions to make the nf-core/sarek code and processing logic more understandable for new contributors and to ensure quality.
+
+### Agent-specific rules
+
+- Keep branches **local** — do NOT push unless explicitly asked
+- Do not amend commits without asking
+- **Don't ask for confirmation** on routine git operations (creating branches, committing) — just do it following the conventions in the guidelines
+- Use `nf-core` tools from the conda environment (`conda activate nf-core`)
+
+### Contributing Principles
+
+- **One PR, one feature** — scope each PR to a single change; keep it as minimal as possible
+- **Read files before editing** — understand existing code before making changes
+- Keep fixes **minimal and focused** — don't refactor surrounding code
+- Don't add docstrings, comments, or type annotations to unchanged code
+- Don't add error handling or validation beyond what's needed
+- Don't over-engineer: no premature abstractions, no feature flags
+- When unsure about scope or approach, ask rather than guess
+
+### Git Workflow
+
+- **Always branch off `origin/dev`**, never master
+- Branch naming: `fix/issue-XXXX` or `feat/issue-XXXX`
+- PRs target the `dev` branch
+- Never force push, never amend published commits without asking
+- Commit messages should be descriptive and include the issue reference
+
+### Codebase Architecture
+
+Sarek follows a hierarchical, modular architecture:
+
+```text
+Modules (atomic processes) → Subworkflows (composed modules) → Workflow (orchestration)
+```
+
+**Key design principles:**
+
+- Separation of concerns between processing steps
+- Reusable components through nf-core modules ecosystem
+- Configuration-driven behavior via `ext.*` directives
+- Comprehensive testing with nf-test
+
+#### Directory Structure
+
+```text
+sarek/
+├── main.nf                     # Pipeline entry point
+├── nextflow.config             # Main configuration
+├── nextflow_schema.json        # Parameter schema (JSON Schema)
+├── modules.json                # nf-core module tracking
+├── modules/
+│   ├── local/                  # Pipeline-specific modules
+│   └── nf-core/                # Imported nf-core modules
+├── subworkflows/
+│   ├── local/                  # Pipeline-specific subworkflows
+│   └── nf-core/                # Imported nf-core subworkflows
+├── workflows/sarek/main.nf     # Main workflow orchestration
+├── conf/
+│   ├── base.config             # Default resource allocations
+│   ├── modules/                # Module-specific configurations
+│   └── test/                   # Test configurations
+├── tests/                      # nf-test test files
+├── docs/                       # Documentation
+└── assets/                     # MultiQC config, samplesheets, etc.
+```
+
+### Code Style
+
+#### Harshil Alignment
+
+Use "Harshil alignment" for include statements - align the closing braces to improve readability:
+
+```groovy
+// CORRECT - Harshil alignment
+include { paramsSummaryMap                                  } from 'plugin/nf-schema'
+include { paramsSummaryMultiqc                              } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
+include { softwareVersionsToYAML                            } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
+include { methodsDescriptionText                            } from '../../subworkflows/local/utils_nfcore_sarek_pipeline'
+
+// CORRECT - With aliases
+include { BAM_CONVERT_SAMTOOLS as CONVERT_FASTQ_INPUT       } from '../../subworkflows/local/bam_convert_samtools'
+include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R1_FQ   } from '../../modules/nf-core/spring/decompress'
+include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R2_FQ   } from '../../modules/nf-core/spring/decompress'
+
+// INCORRECT - No alignment
+include { paramsSummaryMap } from 'plugin/nf-schema'
+include { paramsSummaryMultiqc } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
+```
+
+#### Harshil Alignment in Take/Emit Blocks
+
+Also apply alignment to `take:` and `emit:` blocks:
+
+```groovy
+take:
+cram          // channel: [mandatory] [ meta, cram, crai ]
+dict          // channel: [optional]  [ meta, dict ]
+fasta         // channel: [mandatory] [ fasta ]
+fasta_fai     // channel: [mandatory] [ fasta_fai ]
+intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ]
+
+emit:
+vcf_ann       // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
+tab_ann
+json_ann
+reports       //    path: *.html
+versions      //    path: versions.yml
+```
+
+#### Channel Naming Conventions
+
+```groovy
+// Initial process output channel
+ch_output_from_<process>
+
+// Intermediate/terminal channels
+ch_<previousprocess>_for_<nextprocess>
+
+// Example
+ch_bam_from_markduplicates
+ch_markduplicates_for_baserecalibrator
+```
+
+#### Topic Channels
+
+We are migrating to **Nextflow topic channels** where possible. Topics allow processes and subworkflows to publish to a named topic without explicit channel wiring.
+
+When installing or updating an nf-core module, check if it publishes `versions` or `multiqc` outputs via topics. If it does, use the topic and remove the explicit `.mix()` wiring for those channels.
+
+```groovy
+// OLD - Explicit version/report collection
+versions = versions.mix(TOOL_A.out.versions)
+ch_multiqc_files = ch_multiqc_files.mix(TOOL_A.out.report)
+
+// NEW - If the module uses topics, remove the .mix() lines above.
+// The module already publishes to the topic internally.
+// Collect from the topic in the top-level workflow:
+//   ch_versions = Channel.topic('versions')
+```
+
+#### General Style
+
+- Use 4-space indentation
+- Put channel operations on separate lines for readability
+- Add comments for complex logic
+- Use descriptive variable names
+
+#### Strict Syntax Mode
+
+**When touching any code in a PR, you must update it to use strict Nextflow syntax.** This ensures gradual modernization of the codebase.
+
+**Required Changes When Modifying Code:**
+
+1. **Use explicit `it` variable or named parameters in closures:**
+
+   ```groovy
+   // CORRECT - Explicit named parameters
+   .map { meta, vcf -> [meta, vcf] }
+
+   // CORRECT - Explicit `it` when single parameter
+   .map { it -> it.baseName }
+
+   // DEPRECATED - Implicit `it`
+   .map { it.baseName }
+   ```
+
+2. **Explicit type declarations where applicable:**
+
+   ```groovy
+   // CORRECT
+   String prefix = "${meta.id}"
+   List<String> args = []
+
+   // AVOID in new code
+   def prefix = "${meta.id}"
+   ```
+
+3. **Use underscore prefix for unused/dropped variables:**
+
+   The underscore prefix convention clearly indicates which variables from a closure are intentionally not used in the output. This makes code review easier and prevents confusion about whether a variable was accidentally omitted.
+
+   ```groovy
+   // CORRECT - Underscore prefix shows vcf is intentionally dropped
+   .map { meta, _vcf, tbi -> [meta, tbi] }
+
+   // CORRECT - Multiple dropped variables
+   .map { meta, _vcf, _tbi, file -> [meta, file] }
+
+   // CORRECT - In join operations
+   .join(other_channel, failOnDuplicate: true, failOnMismatch: true)
+       .map { meta, file1, _file2 -> [meta, file1] }
+
+   // CORRECT - When extracting from complex structures
+   VCF_ANNOTATE_SNPEFF.out.vcf_tbi.map { meta, vcf_, _tbi -> [meta, vcf_, []] }
+
+   // INCORRECT - Unclear which variables are intentionally unused
+   .map { meta, vcf, tbi -> [meta, tbi] }
+   ```
+
+   **When to use underscore prefix:**
+   - Variable is received but not included in output
+   - Variable is needed for destructuring but value is discarded
+   - Makes intent clear during code review
+
+### Channel Operations and Gotchas
+
+#### Join Operations - ALWAYS Use `failOnDuplicate` and `failOnMismatch`
+
+When joining channels, ALWAYS specify `failOnDuplicate: true, failOnMismatch: true` to catch bugs early:
+
+```groovy
+// CORRECT - Will fail fast if there are issues
+vcf_tbi = vcf.join(tbi, failOnDuplicate: true, failOnMismatch: true)
+
+// INCORRECT - Silent failures can cause subtle bugs
+vcf_tbi = vcf.join(tbi)
+```
+
+Use `remainder: true` only when intentionally handling unmatched items:
+
+```groovy
+// When some items may not have matches (intentional)
+all_unmapped_bam = SAMTOOLS_VIEW_UNMAP_UNMAP.out.bam
+    .join(SAMTOOLS_VIEW_UNMAP_MAP.out.bam, failOnDuplicate: true, remainder: true)
+    .join(SAMTOOLS_VIEW_MAP_UNMAP.out.bam, failOnDuplicate: true, remainder: true)
+```
+
+#### Branch Operations
+
+Use `branch` to split channels based on conditions:
+
+```groovy
+vcf_out = STRELKA_SINGLE.out.vcf.branch{
+    // Use meta.num_intervals to assess number of intervals
+    intervals:    it[0].num_intervals > 1
+    no_intervals: it[0].num_intervals <= 1
+}
+
+// Access branches
+vcf_out.intervals      // Items where num_intervals > 1
+vcf_out.no_intervals   // Items where num_intervals <= 1
+```
+
+#### GroupTuple - Use `groupKey` for Performance
+
+When using `groupTuple`, use `groupKey` with known size to avoid blocking:
+
+```groovy
+// CORRECT - Non-blocking when size is known
+vcf_to_merge = vcf_out.intervals
+    .map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ]}
+    .groupTuple()
+
+// NOTE: Without groupKey and size, groupTuple is a blocking operation
+// This can cause pipeline hangs if the expected number of items varies
+```
+
+#### Strelka Special Case - SNV and Indel VCFs
+
+Strelka produces TWO VCF files (SNVs and Indels) that need special handling:
+
+```groovy
+// Strelka somatic outputs need to be concatenated before consensus calling
+ch_vcfs = vcfs.branch{ meta, vcf, tbi ->
+    strelka_somatic: meta.variantcaller == 'strelka' && meta.status == '1'
+    other: true
+}
+
+// Concatenate the two strelka VCFs (SNPs and indels) using groupTuple(size: 2)
+BCFTOOLS_CONCAT(ch_vcfs.strelka_somatic.groupTuple(size: 2))
+```
+
+#### Combine vs Join
+
+- Use `join` when combining channels by a key (meta map)
+- Use `combine` when creating cartesian product (e.g., sample x intervals)
+
+```groovy
+// Join by meta key
+vcf_tbi = vcf.join(tbi, failOnDuplicate: true, failOnMismatch: true)
+
+// Combine all samples with all intervals (cartesian product)
+cram_intervals = cram.combine(intervals)
+```
+
+#### Controlling Flow with Channel Operations (Preferred)
+
+**Nextflow is a dataflow language.** Prefer channel operations over `if` statements to control which processes run:
+
+```groovy
+// BEST - Use filter to control what enters a process
+input_channel
+    .filter { meta, _file -> params.tools?.split(',')?.contains('toolname') }
+    .set { ch_for_tool }
+
+TOOL_PROCESS(ch_for_tool)
+
+// BEST - Use branch for multiple conditional paths
+input_channel.branch { meta, file ->
+    tool_a: params.tools?.split(',')?.contains('tool_a')
+    tool_b: params.tools?.split(',')?.contains('tool_b')
+    other: true
+}.set { ch_branched }
+
+TOOL_A(ch_branched.tool_a)
+TOOL_B(ch_branched.tool_b)
+
+// AVOID - if statements for flow control (use only when channel ops aren't suitable)
+if (params.run_tool) {
+    TOOL_PROCESS(input_channel)
+}
+```
+
+**Benefits of channel operations:**
+
+- More idiomatic Nextflow - data drives execution
+- Better composability and testability
+- Clearer dataflow visualization
+- Avoids caching issues when conditions change
+
+### Meta Map Handling
+
+#### Adding Fields to Meta
+
+Use `meta + [key: value]` syntax:
+
+```groovy
+// Add single field
+meta = meta + [id: meta.sample]
+
+// Add multiple fields
+meta = meta + [id: "${meta.sample}-${meta.lane}".toString(), data_type: "fastq_gz", num_lanes: num_lanes.toInteger()]
+
+// In map operation
+.map{ meta, vcf -> [ meta + [ variantcaller:'strelka' ], vcf ] }
+```
+
+#### Removing Fields from Meta - Use `subMap`
+
+Use `meta - meta.subMap('field')` to remove fields:
+
+```groovy
+// Remove single field
+.map{ meta, vcf -> [ meta - meta.subMap('num_intervals'), vcf ] }
+
+// Remove multiple fields
+.map{ meta, vcf, tbi ->
+    [meta - meta.subMap('variantcaller', 'contamination', 'filename'), vcf, tbi]
+}
+
+// Add and remove in one operation
+.map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], vcf ] }
+```
+
+#### Accessing Meta Fields
+
+```groovy
+// In map closures
+.map{ meta, file -> [meta.sample, file] }
+
+// In branch conditions
+.branch{ meta, vcf ->
+    intervals:    meta.num_intervals > 1
+    no_intervals: meta.num_intervals <= 1
+}
+
+// Getting subset of meta
+[meta.patient, meta.subMap('sample', 'status')]
+```
+
+#### Common Meta Fields in Sarek
+
+| Field                | Description                                   |
+| -------------------- | --------------------------------------------- |
+| `meta.patient`       | Patient identifier                            |
+| `meta.sample`        | Sample identifier                             |
+| `meta.status`        | 0 = normal, 1 = tumor                         |
+| `meta.lane`          | Sequencing lane                               |
+| `meta.id`            | Unique identifier (often `${sample}-${lane}`) |
+| `meta.data_type`     | Input type: `fastq_gz`, `bam`, `cram`         |
+| `meta.num_intervals` | Number of intervals for scatter/gather        |
+| `meta.variantcaller` | Name of variant caller                        |
+| `meta.num_lanes`     | Total number of lanes for sample              |
+
+### Modules
+
+#### DEPRECATED: The `ext.when` Clause Pattern
+
+> **DEPRECATED:** The `ext.when` clause pattern is deprecated and should NOT be used in new code. Existing code using this pattern should be refactored when touched in a PR.
+
+You may see comments in older subworkflow files like:
+
+```groovy
+// For all modules here:
+// A when clause condition is defined in the conf/modules.config to determine if the module should be run
+```
+
+**Do not follow this pattern for new code.** Instead, use channel operations to control dataflow — see [Controlling Flow with Channel Operations](#controlling-flow-with-channel-operations-preferred).
+
+The deprecated pattern in config files:
+
+```groovy
+// DEPRECATED - Using ext.when in config
+// withName: 'TOOL_PROCESS' {
+//     ext.when = { params.tools && params.tools.split(',').contains('toolname') }
+// }
+```
+
+When touching existing code with `ext.when`, refactor to use channel operations (`filter`, `branch`) instead.
+
+#### Remapping Channels for Module Input
+
+When a module expects different input structure, remap in the call:
+
+```groovy
+// Remap channel to match module/subworkflow input signature
+BAM_VARIANT_CALLING_CNVKIT(
+    cram.map{ meta, cram, crai -> [ meta, [], cram ] },
+    fasta,
+    fasta_fai,
+    intervals_bed_combined.map{it -> it ? [[id:it[0].baseName], it]: [[id:'no_intervals'], []]},
+    params.cnvkit_reference ? cnvkit_reference.map{ it -> [[id:it[0].baseName], it] } : [[:],[]]
+)
+```
+
+#### Module Memory Requirements
+
+Some modules have specific memory requirements noted in comments:
+
+```groovy
+// In modules/nf-core/bwa/index/main.nf:
+// NOTE requires 5.37N memory where N is the size of the database
+
+// In modules/nf-core/bwamem2/index/main.nf:
+// NOTE Requires 28N GB memory where N is the size of the reference sequence, floor of 280M
+```
+
+#### Adding/Updating nf-core Modules
+
+```bash
+# Install a new module
+nf-core modules install <tool>/<subcommand>
+
+# Update an existing module
+nf-core modules update <tool>/<subcommand>
+
+# List installed modules
+nf-core modules list local
+```
+
+#### Updating VEP modules
+
+When updating `ensemblvep/vep` module, always update the `vep_version` parameter to match the new VEP version. This parameter is used by the LoFTEE plugin to locate the VEP installation path (e.g. `/opt/conda/share/ensembl-vep-${vep_version}`).
+
+Also update `vep_cache_version` in `conf/igenomes.config` for available genomes, based on what's available on [annotation-cache](https://annotation-cache.github.io/ensemblvep/). Not all genomes may have a cache for the new version — only update those that do.
+
+### Subworkflows
+
+#### Subworkflow Naming Patterns
+
+| Category        | Naming Pattern          | Examples                                  |
+| --------------- | ----------------------- | ----------------------------------------- |
+| Alignment       | `fq_align_*`            | `fq_align_bwamem`, `fq_align_bwamem2`     |
+| BAM processing  | `bam_*`                 | `bam_markduplicates`, `bam_applybqsr`     |
+| Variant calling | `bam_variant_calling_*` | `bam_variant_calling_germline_all`        |
+| VCF processing  | `vcf_*`                 | `vcf_annotate_all`, `vcf_concat_variants` |
+| Preparation     | `prepare_*`             | `prepare_genome`, `prepare_intervals`     |
+
+#### Subworkflow Structure
+
+```groovy
+//
+// DESCRIPTION OF SUBWORKFLOW
+//
+
+include { MODULE_A                    } from '../../../modules/nf-core/module_a'
+include { MODULE_B                    } from '../../../modules/nf-core/module_b'
+include { MODULE_B as MODULE_B_ALIAS  } from '../../../modules/nf-core/module_b'
+
+workflow SUBWORKFLOW_NAME {
+    take:
+    input_channel    // channel: [mandatory] [ meta, file ]
+    other_inputs     // channel: [optional]  description
+
+    main:
+    versions = Channel.empty()
+
+    // Initialize output channels
+    output_a = Channel.empty()
+    output_b = Channel.empty()
+
+    // PREFERRED: Use channel operations to control dataflow
+    ch_for_module_a = input_channel
+        .filter { meta, _file -> meta.run_module_a }
+
+    MODULE_A(ch_for_module_a)
+    versions = versions.mix(MODULE_A.out.versions)
+
+    MODULE_B(MODULE_A.out.result)
+    versions = versions.mix(MODULE_B.out.versions)
+
+    emit:
+    result   = MODULE_B.out.result  // channel: [ val(meta), file ]
+    versions                        // channel: versions.yml
+}
+```
+
+#### Scatter-Gather Pattern
+
+Common pattern for parallelizing over intervals:
+
+```groovy
+// Combine samples with intervals for scatter strategy
+cram_intervals = cram.combine(intervals)
+    // Move num_intervals to meta map for later grouping
+    .map{ meta, cram, crai, intervals, intervals_index, num_intervals ->
+        [ meta + [ num_intervals:num_intervals ], cram, crai, intervals, intervals_index ]
+    }
+
+// Run process on each interval
+PROCESS(cram_intervals, fasta, fasta_fai)
+
+// Gather: Branch by whether intervals were used
+vcf_out = PROCESS.out.vcf.branch{
+    intervals:    it[0].num_intervals > 1
+    no_intervals: it[0].num_intervals <= 1
+}
+
+// Merge interval results
+vcf_to_merge = vcf_out.intervals
+    .map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ]}
+    .groupTuple()
+
+MERGE_VCFS(vcf_to_merge, dict)
+
+// Combine merged and non-interval results, clean up meta
+vcf_final = Channel.empty()
+    .mix(MERGE_VCFS.out.vcf, vcf_out.no_intervals)
+    .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'toolname' ], vcf ] }
+```
+
+### Configuration
+
+#### Module Configuration Files
+
+Module behavior is controlled via `conf/modules/<tool>.config`:
+
+```groovy
+process {
+    withName: 'NEWTOOL_PROCESS' {
+        ext.args   = { params.newtool_args ?: '' }
+        ext.prefix = { "${meta.id}.newtool" }
+        publishDir = [
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}/variant_calling/newtool/${meta.id}/" },
+            pattern: "*{vcf.gz,vcf.gz.tbi}"
+        ]
+    }
+}
+```
+
+> **Note:** Older config files wrap process blocks in `if (params.tools && params.tools.split(',').contains('tool'))` guards. Do **not** use this pattern in new code — control which processes run via channel operations (`filter`, `branch`) in the workflow/subworkflow instead. When touching existing config files, remove these guards.
+
+#### Resource Labels
+
+Use standard nf-core labels in `conf/base.config`:
+
+| Label                 | CPUs | Memory | Time |
+| --------------------- | ---- | ------ | ---- |
+| `process_single`      | 1    | 6 GB   | 8 h  |
+| `process_low`         | 2    | 12 GB  | 8 h  |
+| `process_medium`      | 6    | 36 GB  | 16 h |
+| `process_high`        | 12   | 72 GB  | 32 h |
+| `process_long`        | -    | -      | 40 h |
+| `process_high_memory` | -    | 200 GB | -    |
+
+#### Adding New Parameters
+
+1. **Add to `nextflow.config`** with default value:
+
+   ```groovy
+   params {
+       new_param = false
+   }
+   ```
+
+2. **Update schema** using nf-core tools:
+
+   ```bash
+   nf-core pipelines schema build
+   ```
+
+3. **Add validation** if needed in the workflow
+
+### Pipeline Testing
+
+#### Running Tests
+
+```bash
+# Run all tests
+nf-test test --profile+=debug,docker --verbose
+
+# Run specific test
+nf-test test tests/variant_calling_haplotypecaller.nf.test --profile+=debug,docker
+
+# Run with stub mode (faster, no actual execution)
+nf-test test tests/default.nf.test --profile+=debug,docker -stub
+
+# Update snapshots when outputs legitimately change
+nf-test test tests/my_test.nf.test --profile+=debug,docker --update-snapshot
+```
+
+#### Test Structure
+
+Tests use a scenario-based pattern powered by `tests/lib/UTILS.groovy`. Each `.nf.test` file defines an array of test scenarios — one map per test case — and `UTILS` handles tagging, params, assertions, and snapshot comparison automatically.
+
+```groovy
+def projectDir = new File('.').absolutePath
+
+nextflow_pipeline {
+    name "Test pipeline"
+    script "../main.nf"
+
+    def test_scenario = [
+        [
+            name: "Test haplotypecaller with WES input",
+            params: [
+                input: "${projectDir}/tests/csv/3.0/mapped_single_bam.csv",
+                step: "variant_calling",
+                tools: 'haplotypecaller',
+                wes: true
+            ],
+            no_conda: true
+        ],
+        [
+            name: "Test with stub",
+            params: [],
+            stub: true
+        ],
+        [
+            name: "Fails with invalid input",
+            params: [
+                input: "${projectDir}/tests/csv/3.0/vcf_single.csv",
+                step: 'annotate',
+                tools: 'vep'
+            ],
+            failure: true
+        ]
+    ]
+
+    test_scenario.each { scenario ->
+        test(scenario.name, UTILS.getTest(scenario))
+    }
+}
+```
+
+`UTILS.getTest(scenario)` returns an nf-test closure that:
+
+1. **Tags the test** automatically based on scenario options (cpu/gpu, conda, stub, failure)
+2. **Sets params** from the scenario map, plus `outdir`
+3. **Asserts success or failure** based on `scenario.failure`
+4. **Generates snapshot assertions** via `UTILS.getAssertions()`, which checks:
+   - Number of succeeded tasks
+   - Software versions YAML
+   - Output file tree stability (file names and directory structure)
+   - BAM/CRAM read md5 checksums
+   - VCF variant md5 checksums (or summary for unstable QUAL scores)
+   - stderr/stdout for unexpected warnings
+
+#### Test Scenario Options
+
+| Option                         | Description                                                         |
+| ------------------------------ | ------------------------------------------------------------------- |
+| `failure`                      | Expect the pipeline to fail (boolean)                               |
+| `gpu`                          | Tag as GPU test (default: cpu)                                      |
+| `ignoreFiles`                  | Files to ignore in assertions                                       |
+| `include_freebayes_unfiltered` | Use VCF summary instead of md5 for freebayes unfiltered output      |
+| `include_muse_txt`             | Include MuSE txt file md5 in assertions                             |
+| `include_varlociraptor_vcf`    | Include varlociraptor VCF in assertions                             |
+| `name`                         | Test name (descriptive, used as nf-test test name)                  |
+| `no_conda`                     | Mark as incompatible with conda (default: conda-compatible)         |
+| `no_vcf_md5sum`                | Use VCF summary instead of md5 for all VCF files                    |
+| `params`                       | Map of Nextflow parameters to set                                   |
+| `sentieon`                     | Tag as Sentieon test (default: cpu)                                 |
+| `snapshot_ignore`              | Additional strings to ignore in snapshot output                     |
+| `snapshot_include`             | Only include lines matching this string in snapshot                 |
+| `snapshot`                     | Capture stderr/stdout: `'stderr'`, `'stdout'`, or `'stderr,stdout'` |
+| `stub`                         | Run in stub mode (boolean)                                          |
+| `tag`                          | Additional custom nf-test tag                                       |
+| `vcf_header_check`             | Assert VCF header contains this string                              |
+
+### Documentation
+
+#### Documentation Requirements
+
+**Any change that affects pipeline output or adds new functionality MUST include documentation updates.**
+
+#### Documentation Files
+
+| File             | Purpose                  | When to Update                                  |
+| ---------------- | ------------------------ | ----------------------------------------------- |
+| `README.md`      | Pipeline overview        | **New tools** (add to overview/tool list)       |
+| `docs/usage.md`  | Usage instructions       | New parameters, new tools, input format changes |
+| `docs/output.md` | Output file descriptions | **Any change to outputs**, new tools            |
+| `CHANGELOG.md`   | Version history          | Every PR                                        |
+| `CITATIONS.md`   | Tool citations           | New tools                                       |
+| `docs/images/`   | Metro maps, diagrams     | **New tools**, workflow changes                 |
+
+#### New Tool Documentation Checklist
+
+When adding a new tool, you **MUST** update ALL of the following:
+
+1. **`README.md`** - Add tool to the pipeline overview/feature list
+2. **`docs/usage.md`** - Document all new parameters and usage instructions
+3. **`docs/output.md`** - Document all output files produced by the tool
+4. **`docs/images/sarek_subway.*`** - Add tool to the metro map (SVG and PNG)
+5. **`CITATIONS.md`** - Add tool citation
+6. **`CHANGELOG.md`** - Document the addition
+
+#### Output Changes Documentation
+
+Any PR that changes pipeline outputs (new files, changed file names, different content) **MUST** update:
+
+1. **`docs/output.md`** - Reflect the new/changed outputs
+2. **`CHANGELOG.md`** - Note the change under appropriate section
+
+#### CHANGELOG Format
+
+Follow [Keep a Changelog](https://keepachangelog.com/) format.
+
+**Important conventions:**
+
+- Entries reference the **PR number**, not the issue number:
+  ```
+  - [#XXX](https://github.com/nf-core/sarek/pull/XXX) - Description of change
+  ```
+- Use `XXX` as placeholder when no PR exists yet
+- The issue number goes in the **PR description body** (for auto-close), not the changelog
+- Entries within each section are in **ascending order** by PR number
+
+```markdown
+## [Unreleased]
+
+### Added
+
+- [#PR_NUMBER](https://github.com/nf-core/sarek/pull/PR_NUMBER) - Description
+
+### Changed
+
+### Fixed
+
+### Removed
+
+### Dependencies
+
+| Dependency | Old version | New version |
+| ---------- | ----------- | ----------- |
+| tool_name  | 1.0.0       | 1.1.0       |
+
+### Parameters
+
+| Params        | status |
+| ------------- | ------ |
+| `--new_param` | New    |
+
+### Developer section
+
+#### Added
+
+#### Changed
+
+#### Fixed
+
+#### Removed
+```
+
+#### Output Documentation
+
+In `docs/output.md`, document each tool's outputs:
+
+```markdown
+### Tool Name
+
+<details markdown="1">
+<summary>Output files</summary>
+
+- `path/to/output/`
+  - `*.extension`: Description of the file
+
+</details>
+
+Brief description of what this tool produces.
+```
+
+### Metro Map Updates
+
+#### Metro Map Files
+
+Located in `docs/images/`:
+
+- `sarek_subway.svg` / `sarek_subway.png` - Main pipeline flow
+- `sarek_indices_subway.svg` / `sarek_indices_subway.png` - Index building flow
+
+#### When to Update
+
+- Adding new tools or variant callers
+- Adding new preprocessing steps
+- Changing the pipeline flow
+- Adding new post-processing options
+
+#### Update Process
+
+1. Edit the SVG file (use Inkscape or similar)
+2. Export to PNG
+3. Follow nf-core [design guidelines](https://nf-co.re/developers/design_guidelines)
+4. After release, checkout figures from `master` to `dev`:
+
+   ```bash
+   git checkout upstream/master -- docs/images/sarek_subway.svg
+   git checkout upstream/master -- docs/images/sarek_subway.png
+   ```
+
+### PR Checklist
+
+#### Before Submitting
+
+- [ ] PR targets `dev` branch (not `master`)
+- [ ] Code follows Harshil alignment style
+- [ ] **Any touched code updated to strict syntax** (explicit closure params, underscore for unused vars)
+- [ ] **No new `ext.when` usage** - use channel operations instead
+- [ ] **Prefer channel operations** (`filter`, `branch`) over `if` statements for flow control
+- [ ] Pre-commit checks pass: `pre-commit run --all-files`
+- [ ] All tests pass: `nf-test test --profile+=debug,docker`
+- [ ] Linting passes: `nf-core pipelines lint`
+- [ ] No debug mode warnings
+
+#### For New Tools
+
+**Code:**
+
+- [ ] Module added/imported correctly
+- [ ] Configuration in `conf/modules/<tool>.config`
+- [ ] Test added in `tests/`
+- [ ] MultiQC config updated (`assets/multiqc_config.yml`) if tool has MultiQC module
+
+**Documentation (ALL required):**
+
+- [ ] `README.md` - Tool added to pipeline overview/feature list
+- [ ] `docs/usage.md` - All parameters documented with usage instructions
+- [ ] `docs/output.md` - All output files documented
+- [ ] `docs/images/sarek_subway.svg` - Tool added to metro map
+- [ ] `docs/images/sarek_subway.png` - Exported PNG of updated metro map
+- [ ] `CITATIONS.md` - Tool citation added
+- [ ] `CHANGELOG.md` - Addition documented
+
+#### For New Variant Callers
+
+Adding a variant caller touches **6 locations** — missing any of them causes silent bugs. All of the above "New Tools" items apply, plus:
+
+- [ ] **`nextflow_schema.json`** - Add to the `tools` parameter regex pattern
+- [ ] **Dispatcher subworkflow** - Add call and wire outputs into `vcf_all.mix(...)`:
+  - `subworkflows/local/bam_variant_calling_germline_all/main.nf` (germline callers)
+  - `subworkflows/local/bam_variant_calling_somatic_all/main.nf` (somatic pair callers)
+  - `subworkflows/local/bam_variant_calling_tumor_only_all/main.nf` (tumor-only callers)
+  - Use channel operations (`filter`, `branch`) to control execution — not `if` blocks (existing `if` blocks are legacy)
+- [ ] **`subworkflows/local/post_variantcalling/main.nf`** - Add to `small_variantcallers` list (for SNV callers eligible for normalization/filtering/consensus) or `excluded_variantcallers` (for SV callers). **Forgetting this silently excludes the caller from post-processing.**
+- [ ] **Individual subworkflow** - Set `variantcaller` in meta map (e.g., `meta + [variantcaller: 'toolname']`)
+
+#### For New Parameters
+
+- [ ] Default value in `nextflow.config`
+- [ ] Schema updated: `nf-core pipelines schema build`
+- [ ] Validation added (if needed)
+- [ ] Documentation in `docs/usage.md`
+- [ ] CHANGELOG updated
+
+#### For Changes Affecting Pipeline Output
+
+Any PR that changes output files (new files, renamed files, changed content):
+
+- [ ] `docs/output.md` updated to reflect changes
+- [ ] `CHANGELOG.md` updated
+- [ ] If significant workflow change: metro map updated (`docs/images/sarek_subway.*`)
+
+### Common Gotchas
+
+#### 1. Forgetting `failOnDuplicate`/`failOnMismatch` on Joins
+
+**Problem:** Silent data loss or incorrect pairing
+**Solution:** Always use `join(..., failOnDuplicate: true, failOnMismatch: true)`
+
+#### 2. Strelka Produces Two VCFs
+
+**Problem:** Strelka outputs SNV and Indel VCFs separately
+**Solution:** Use `groupTuple(size: 2)` then `BCFTOOLS_CONCAT` before downstream processing
+
+#### 3. Blocking GroupTuple
+
+**Problem:** `groupTuple()` without size blocks pipeline
+**Solution:** Use `groupKey(meta, meta.num_intervals)` when size is known
+
+#### 4. Meta Fields Persisting
+
+**Problem:** Temporary meta fields (like `num_intervals`) persist in output
+**Solution:** Clean up with `meta - meta.subMap('field_name')` before emit
+
+#### 5. DeepVariant Conda
+
+**Problem:** DeepVariant doesn't support Conda
+**Solution:** Note in module: `// FIXME Conda is not supported at the moment`
+
+#### 6. BWA Memory Requirements
+
+**Problem:** Unexpected OOM errors
+**Solution:** BWA requires ~5.37N memory, BWA-MEM2 requires ~28N GB where N = reference size
+
+#### 7. Using Deprecated `ext.when` Pattern
+
+**Problem:** Old code uses `ext.when` in config to control module execution
+**Solution:** When touching this code, refactor to use channel operations (`filter`, `branch`) to control dataflow. Avoid both `ext.when` AND `if` statements where possible.
+
+#### 8. Forgetting to Register a New Variant Caller
+
+**Problem:** New variant caller runs and produces VCFs, but is silently excluded from normalization, filtering, and consensus calling
+**Solution:** Must update all 6 registration points — see [For New Variant Callers](#for-new-variant-callers) checklist. The most commonly missed is `post_variantcalling/main.nf`'s `small_variantcallers` list.
+
+#### 9. Implicit Variables in Closures
+
+**Problem:** Using implicit `it` makes code harder to read and review
+**Solution:** Always use explicit named parameters in closures: `.map { meta, vcf -> ... }` not `.map { it[0], it[1] -> ... }`
+
+### Getting Help
+
+- **Slack:** [#sarek channel](https://nfcore.slack.com/channels/sarek)
+- **GitHub Issues:** [nf-core/sarek/issues](https://github.com/nf-core/sarek/issues)
+- **Documentation:** [nf-co.re/sarek](https://nf-co.re/sarek)
+
+### Quick Reference
+
+#### Essential Commands
+
+```bash
+# Run tests
+nf-test test --profile=+debug,docker
+
+# Lint pipeline
+nf-core pipelines lint
+
+# Update schema
+nf-core pipelines schema build
+
+# Install/update module
+nf-core modules install <tool>/<subcommand>
+nf-core modules update <tool>/<subcommand>
+```
+
+#### Key Files for Common Changes
+
+| Change Type   | Primary Files                                   |
+| ------------- | ----------------------------------------------- |
+| New parameter | `nextflow.config`, `nextflow_schema.json`       |
+| New tool      | `conf/modules/<tool>.config`, subworkflow, test |
+| Bug fix       | Relevant module/subworkflow, test               |
+| Documentation | `docs/usage.md`, `docs/output.md`               |
+| Any change    | `CHANGELOG.md`                                  |
diff --git a/docs/DEVELOPER_GUIDELINES.md b/docs/DEVELOPER_GUIDELINES.md
deleted file mode 100644
index 9710248b23..0000000000
--- a/docs/DEVELOPER_GUIDELINES.md
+++ /dev/null
@@ -1,1009 +0,0 @@
-# nf-core/sarek Developer Guidelines
-
-This document provides comprehensive guidelines for contributing to the nf-core/sarek pipeline. These guidelines are designed for both human developers and AI agents.
-
-## Table of Contents
-
-- [Contributing Principles](#contributing-principles)
-- [Git Workflow](#git-workflow)
-- [Codebase Architecture](#codebase-architecture)
-- [Code Style](#code-style)
-- [Channel Operations and Gotchas](#channel-operations-and-gotchas)
-- [Meta Map Handling](#meta-map-handling)
-- [Modules](#modules)
-- [Subworkflows](#subworkflows)
-- [Configuration](#configuration)
-- [Testing](#testing)
-- [Documentation](#documentation)
-- [Metro Map Updates](#metro-map-updates)
-- [PR Checklist](#pr-checklist)
-
----
-
-## Contributing Principles
-
-- **One PR, one feature** — scope each PR to a single change; keep it as minimal as possible
-- **Read files before editing** — understand existing code before making changes
-- Keep fixes **minimal and focused** — don't refactor surrounding code
-- Don't add docstrings, comments, or type annotations to unchanged code
-- Don't add error handling or validation beyond what's needed
-- Don't over-engineer: no premature abstractions, no feature flags
-- When unsure about scope or approach, ask rather than guess
-
----
-
-## Git Workflow
-
-- **Always branch off `origin/dev`**, never master
-- Branch naming: `fix/issue-XXXX` or `feat/issue-XXXX`
-- PRs target the `dev` branch
-- Never force push, never amend published commits without asking
-- Commit messages should be descriptive and include the issue reference
-
----
-
-## Codebase Architecture
-
-Sarek follows a hierarchical, modular architecture:
-
-```
-Modules (atomic processes) → Subworkflows (composed modules) → Workflow (orchestration)
-```
-
-**Key design principles:**
-
-- Separation of concerns between processing steps
-- Reusable components through nf-core modules ecosystem
-- Configuration-driven behavior via `ext.*` directives
-- Comprehensive testing with nf-test
-
-### Directory Structure
-
-```
-sarek/
-├── main.nf                     # Pipeline entry point
-├── nextflow.config             # Main configuration
-├── nextflow_schema.json        # Parameter schema (JSON Schema)
-├── modules.json                # nf-core module tracking
-├── modules/
-│   ├── local/                  # Pipeline-specific modules
-│   └── nf-core/                # Imported nf-core modules
-├── subworkflows/
-│   ├── local/                  # Pipeline-specific subworkflows
-│   └── nf-core/                # Imported nf-core subworkflows
-├── workflows/sarek/main.nf     # Main workflow orchestration
-├── conf/
-│   ├── base.config             # Default resource allocations
-│   ├── modules/                # Module-specific configurations
-│   └── test/                   # Test configurations
-├── tests/                      # nf-test test files
-├── docs/                       # Documentation
-└── assets/                     # MultiQC config, samplesheets, etc.
-```
-
----
-
-## Code Style
-
-### Harshil Alignment
-
-Use "Harshil alignment" for include statements - align the closing braces to improve readability:
-
-```groovy
-// CORRECT - Harshil alignment
-include { paramsSummaryMap                                  } from 'plugin/nf-schema'
-include { paramsSummaryMultiqc                              } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
-include { softwareVersionsToYAML                            } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
-include { methodsDescriptionText                            } from '../../subworkflows/local/utils_nfcore_sarek_pipeline'
-
-// CORRECT - With aliases
-include { BAM_CONVERT_SAMTOOLS as CONVERT_FASTQ_INPUT       } from '../../subworkflows/local/bam_convert_samtools'
-include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R1_FQ   } from '../../modules/nf-core/spring/decompress'
-include { SPRING_DECOMPRESS as SPRING_DECOMPRESS_TO_R2_FQ   } from '../../modules/nf-core/spring/decompress'
-
-// INCORRECT - No alignment
-include { paramsSummaryMap } from 'plugin/nf-schema'
-include { paramsSummaryMultiqc } from '../../subworkflows/nf-core/utils_nfcore_pipeline'
-```
-
-### Harshil Alignment in Take/Emit Blocks
-
-Also apply alignment to `take:` and `emit:` blocks:
-
-```groovy
-take:
-cram          // channel: [mandatory] [ meta, cram, crai ]
-dict          // channel: [optional]  [ meta, dict ]
-fasta         // channel: [mandatory] [ fasta ]
-fasta_fai     // channel: [mandatory] [ fasta_fai ]
-intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ]
-
-emit:
-vcf_ann       // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
-tab_ann
-json_ann
-reports       //    path: *.html
-versions      //    path: versions.yml
-```
-
-### Channel Naming Conventions
-
-```groovy
-// Initial process output channel
-ch_output_from_<process>
-
-// Intermediate/terminal channels
-ch_<previousprocess>_for_<nextprocess>
-
-// Example
-ch_bam_from_markduplicates
-ch_markduplicates_for_baserecalibrator
-```
-
-### Topic Channels
-
-We are migrating to **Nextflow topic channels** where possible. Topics allow processes and subworkflows to publish to a named topic without explicit channel wiring.
-
-When installing or updating an nf-core module, check if it publishes `versions` or `multiqc` outputs via topics. If it does, use the topic and remove the explicit `.mix()` wiring for those channels.
-
-```groovy
-// OLD - Explicit version/report collection
-versions = versions.mix(TOOL_A.out.versions)
-ch_multiqc_files = ch_multiqc_files.mix(TOOL_A.out.report)
-
-// NEW - If the module uses topics, remove the .mix() lines above.
-// The module already publishes to the topic internally.
-// Collect from the topic in the top-level workflow:
-//   ch_versions = Channel.topic('versions')
-```
-
-### General Style
-
-- Use 4-space indentation
-- Put channel operations on separate lines for readability
-- Add comments for complex logic
-- Use descriptive variable names
-
-### Strict Syntax Mode
-
-**When touching any code in a PR, you must update it to use strict Nextflow syntax.** This ensures gradual modernization of the codebase.
-
-#### Required Changes When Modifying Code
-
-1. **Use explicit `it` variable or named parameters in closures:**
-
-   ```groovy
-   // CORRECT - Explicit named parameters
-   .map { meta, vcf -> [meta, vcf] }
-
-   // CORRECT - Explicit `it` when single parameter
-   .map { it -> it.baseName }
-
-   // DEPRECATED - Implicit `it`
-   .map { it.baseName }
-   ```
-
-2. **Explicit type declarations where applicable:**
-
-   ```groovy
-   // CORRECT
-   String prefix = "${meta.id}"
-   List<String> args = []
-
-   // AVOID in new code
-   def prefix = "${meta.id}"
-   ```
-
-3. **Use underscore prefix for unused/dropped variables:**
-
-   The underscore prefix convention clearly indicates which variables from a closure are intentionally not used in the output. This makes code review easier and prevents confusion about whether a variable was accidentally omitted.
-
-   ```groovy
-   // CORRECT - Underscore prefix shows vcf is intentionally dropped
-   .map { meta, _vcf, tbi -> [meta, tbi] }
-
-   // CORRECT - Multiple dropped variables
-   .map { meta, _vcf, _tbi, file -> [meta, file] }
-
-   // CORRECT - In join operations
-   .join(other_channel, failOnDuplicate: true, failOnMismatch: true)
-       .map { meta, file1, _file2 -> [meta, file1] }
-
-   // CORRECT - When extracting from complex structures
-   VCF_ANNOTATE_SNPEFF.out.vcf_tbi.map { meta, vcf_, _tbi -> [meta, vcf_, []] }
-
-   // INCORRECT - Unclear which variables are intentionally unused
-   .map { meta, vcf, tbi -> [meta, tbi] }
-   ```
-
-   **When to use underscore prefix:**
-   - Variable is received but not included in output
-   - Variable is needed for destructuring but value is discarded
-   - Makes intent clear during code review
-
----
-
-## Channel Operations and Gotchas
-
-### Join Operations - ALWAYS Use `failOnDuplicate` and `failOnMismatch`
-
-When joining channels, ALWAYS specify `failOnDuplicate: true, failOnMismatch: true` to catch bugs early:
-
-```groovy
-// CORRECT - Will fail fast if there are issues
-vcf_tbi = vcf.join(tbi, failOnDuplicate: true, failOnMismatch: true)
-
-// INCORRECT - Silent failures can cause subtle bugs
-vcf_tbi = vcf.join(tbi)
-```
-
-Use `remainder: true` only when intentionally handling unmatched items:
-
-```groovy
-// When some items may not have matches (intentional)
-all_unmapped_bam = SAMTOOLS_VIEW_UNMAP_UNMAP.out.bam
-    .join(SAMTOOLS_VIEW_UNMAP_MAP.out.bam, failOnDuplicate: true, remainder: true)
-    .join(SAMTOOLS_VIEW_MAP_UNMAP.out.bam, failOnDuplicate: true, remainder: true)
-```
-
-### Branch Operations
-
-Use `branch` to split channels based on conditions:
-
-```groovy
-vcf_out = STRELKA_SINGLE.out.vcf.branch{
-    // Use meta.num_intervals to assess number of intervals
-    intervals:    it[0].num_intervals > 1
-    no_intervals: it[0].num_intervals <= 1
-}
-
-// Access branches
-vcf_out.intervals      // Items where num_intervals > 1
-vcf_out.no_intervals   // Items where num_intervals <= 1
-```
-
-### GroupTuple - Use `groupKey` for Performance
-
-When using `groupTuple`, use `groupKey` with known size to avoid blocking:
-
-```groovy
-// CORRECT - Non-blocking when size is known
-vcf_to_merge = vcf_out.intervals
-    .map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ]}
-    .groupTuple()
-
-// NOTE: Without groupKey and size, groupTuple is a blocking operation
-// This can cause pipeline hangs if the expected number of items varies
-```
-
-### Strelka Special Case - SNV and Indel VCFs
-
-Strelka produces TWO VCF files (SNVs and Indels) that need special handling:
-
-```groovy
-// Strelka somatic outputs need to be concatenated before consensus calling
-ch_vcfs = vcfs.branch{ meta, vcf, tbi ->
-    strelka_somatic: meta.variantcaller == 'strelka' && meta.status == '1'
-    other: true
-}
-
-// Concatenate the two strelka VCFs (SNPs and indels) using groupTuple(size: 2)
-BCFTOOLS_CONCAT(ch_vcfs.strelka_somatic.groupTuple(size: 2))
-```
-
-### Combine vs Join
-
-- Use `join` when combining channels by a key (meta map)
-- Use `combine` when creating cartesian product (e.g., sample x intervals)
-
-```groovy
-// Join by meta key
-vcf_tbi = vcf.join(tbi, failOnDuplicate: true, failOnMismatch: true)
-
-// Combine all samples with all intervals (cartesian product)
-cram_intervals = cram.combine(intervals)
-```
-
-### Controlling Flow with Channel Operations (Preferred)
-
-**Nextflow is a dataflow language.** Prefer channel operations over `if` statements to control which processes run:
-
-```groovy
-// BEST - Use filter to control what enters a process
-input_channel
-    .filter { meta, _file -> params.tools?.split(',')?.contains('toolname') }
-    .set { ch_for_tool }
-
-TOOL_PROCESS(ch_for_tool)
-
-// BEST - Use branch for multiple conditional paths
-input_channel.branch { meta, file ->
-    tool_a: params.tools?.split(',')?.contains('tool_a')
-    tool_b: params.tools?.split(',')?.contains('tool_b')
-    other: true
-}.set { ch_branched }
-
-TOOL_A(ch_branched.tool_a)
-TOOL_B(ch_branched.tool_b)
-
-// AVOID - if statements for flow control (use only when channel ops aren't suitable)
-if (params.run_tool) {
-    TOOL_PROCESS(input_channel)
-}
-```
-
-**Benefits of channel operations:**
-
-- More idiomatic Nextflow - data drives execution
-- Better composability and testability
-- Clearer dataflow visualization
-- Avoids caching issues when conditions change
-
----
-
-## Meta Map Handling
-
-### Adding Fields to Meta
-
-Use `meta + [key: value]` syntax:
-
-```groovy
-// Add single field
-meta = meta + [id: meta.sample]
-
-// Add multiple fields
-meta = meta + [id: "${meta.sample}-${meta.lane}".toString(), data_type: "fastq_gz", num_lanes: num_lanes.toInteger()]
-
-// In map operation
-.map{ meta, vcf -> [ meta + [ variantcaller:'strelka' ], vcf ] }
-```
-
-### Removing Fields from Meta - Use `subMap`
-
-Use `meta - meta.subMap('field')` to remove fields:
-
-```groovy
-// Remove single field
-.map{ meta, vcf -> [ meta - meta.subMap('num_intervals'), vcf ] }
-
-// Remove multiple fields
-.map{ meta, vcf, tbi ->
-    [meta - meta.subMap('variantcaller', 'contamination', 'filename'), vcf, tbi]
-}
-
-// Add and remove in one operation
-.map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], vcf ] }
-```
-
-### Accessing Meta Fields
-
-```groovy
-// In map closures
-.map{ meta, file -> [meta.sample, file] }
-
-// In branch conditions
-.branch{ meta, vcf ->
-    intervals:    meta.num_intervals > 1
-    no_intervals: meta.num_intervals <= 1
-}
-
-// Getting subset of meta
-[meta.patient, meta.subMap('sample', 'status')]
-```
-
-### Common Meta Fields in Sarek
-
-| Field                | Description                                   |
-| -------------------- | --------------------------------------------- |
-| `meta.patient`       | Patient identifier                            |
-| `meta.sample`        | Sample identifier                             |
-| `meta.status`        | 0 = normal, 1 = tumor                         |
-| `meta.lane`          | Sequencing lane                               |
-| `meta.id`            | Unique identifier (often `${sample}-${lane}`) |
-| `meta.data_type`     | Input type: `fastq_gz`, `bam`, `cram`         |
-| `meta.num_intervals` | Number of intervals for scatter/gather        |
-| `meta.variantcaller` | Name of variant caller                        |
-| `meta.num_lanes`     | Total number of lanes for sample              |
-
----
-
-## Modules
-
-### DEPRECATED: The `ext.when` Clause Pattern
-
-> **DEPRECATED:** The `ext.when` clause pattern is deprecated and should NOT be used in new code. Existing code using this pattern should be refactored when touched in a PR.
-
-You may see comments in older subworkflow files like:
-
-```groovy
-// For all modules here:
-// A when clause condition is defined in the conf/modules.config to determine if the module should be run
-```
-
-**Do not follow this pattern for new code.** Instead, prefer channel operations to control dataflow:
-
-```groovy
-// BEST - Use channel operations (filter, branch) to control dataflow
-input_channel
-    .filter { meta, _file -> params.tools?.split(',')?.contains('toolname') }
-    .set { ch_for_tool }
-
-TOOL_PROCESS(ch_for_tool)
-
-// ACCEPTABLE - When channel operations aren't suitable, use explicit conditional
-if (tools && tools.split(',').contains('toolname')) {
-    TOOL_PROCESS(input_channel)
-    versions = versions.mix(TOOL_PROCESS.out.versions)
-}
-
-// DEPRECATED - Using ext.when in config
-// withName: 'TOOL_PROCESS' {
-//     ext.when = { params.tools && params.tools.split(',').contains('toolname') }
-// }
-```
-
-**Why channel operations are preferred:**
-
-- Nextflow is a dataflow language - let the data drive execution
-- Channel operations are more composable and testable
-- Avoids issues with process caching when conditions change
-- Makes the pipeline logic more explicit and traceable
-
-### Remapping Channels for Module Input
-
-When a module expects different input structure, remap in the call:
-
-```groovy
-// Remap channel to match module/subworkflow input signature
-BAM_VARIANT_CALLING_CNVKIT(
-    cram.map{ meta, cram, crai -> [ meta, [], cram ] },
-    fasta,
-    fasta_fai,
-    intervals_bed_combined.map{it -> it ? [[id:it[0].baseName], it]: [[id:'no_intervals'], []]},
-    params.cnvkit_reference ? cnvkit_reference.map{ it -> [[id:it[0].baseName], it] } : [[:],[]]
-)
-```
-
-### Module Memory Requirements
-
-Some modules have specific memory requirements noted in comments:
-
-```groovy
-// In modules/nf-core/bwa/index/main.nf:
-// NOTE requires 5.37N memory where N is the size of the database
-
-// In modules/nf-core/bwamem2/index/main.nf:
-// NOTE Requires 28N GB memory where N is the size of the reference sequence, floor of 280M
-```
-
-### Adding/Updating nf-core Modules
-
-```bash
-# Install a new module
-nf-core modules install <tool>/<subcommand>
-
-# Update an existing module
-nf-core modules update <tool>/<subcommand>
-
-# List installed modules
-nf-core modules list local
-```
-
-### Updating VEP modules
-
-When updating `ensemblvep/vep` module, always update the `vep_version` parameter to match the new VEP version. This parameter is used by the LoFTEE plugin to locate the VEP installation path (e.g. `/opt/conda/share/ensembl-vep-${vep_version}`).
-
-Also update `vep_cache_version` in `conf/igenomes.config` for available genomes, based on what's available on [annotation-cache](https://annotation-cache.github.io/ensemblvep/). Not all genomes may have a cache for the new version — only update those that do.
-
----
-
-## Subworkflows
-
-### Subworkflow Naming Patterns
-
-| Category        | Naming Pattern          | Examples                                  |
-| --------------- | ----------------------- | ----------------------------------------- |
-| Alignment       | `fq_align_*`            | `fq_align_bwamem`, `fq_align_bwamem2`     |
-| BAM processing  | `bam_*`                 | `bam_markduplicates`, `bam_applybqsr`     |
-| Variant calling | `bam_variant_calling_*` | `bam_variant_calling_germline_all`        |
-| VCF processing  | `vcf_*`                 | `vcf_annotate_all`, `vcf_concat_variants` |
-| Preparation     | `prepare_*`             | `prepare_genome`, `prepare_intervals`     |
-
-### Subworkflow Structure
-
-```groovy
-//
-// DESCRIPTION OF SUBWORKFLOW
-//
-
-include { MODULE_A                    } from '../../../modules/nf-core/module_a'
-include { MODULE_B                    } from '../../../modules/nf-core/module_b'
-include { MODULE_B as MODULE_B_ALIAS  } from '../../../modules/nf-core/module_b'
-
-workflow SUBWORKFLOW_NAME {
-    take:
-    input_channel    // channel: [mandatory] [ meta, file ]
-    other_inputs     // channel: [optional]  description
-
-    main:
-    versions = Channel.empty()
-
-    // Initialize output channels
-    output_a = Channel.empty()
-    output_b = Channel.empty()
-
-    // PREFERRED: Use channel operations to control dataflow
-    ch_for_module_a = input_channel
-        .filter { meta, _file -> meta.run_module_a }
-
-    MODULE_A(ch_for_module_a)
-    versions = versions.mix(MODULE_A.out.versions)
-
-    MODULE_B(MODULE_A.out.result)
-    versions = versions.mix(MODULE_B.out.versions)
-
-    emit:
-    result   = MODULE_B.out.result  // channel: [ val(meta), file ]
-    versions                        // channel: versions.yml
-}
-```
-
-### Scatter-Gather Pattern
-
-Common pattern for parallelizing over intervals:
-
-```groovy
-// Combine samples with intervals for scatter strategy
-cram_intervals = cram.combine(intervals)
-    // Move num_intervals to meta map for later grouping
-    .map{ meta, cram, crai, intervals, intervals_index, num_intervals ->
-        [ meta + [ num_intervals:num_intervals ], cram, crai, intervals, intervals_index ]
-    }
-
-// Run process on each interval
-PROCESS(cram_intervals, fasta, fasta_fai)
-
-// Gather: Branch by whether intervals were used
-vcf_out = PROCESS.out.vcf.branch{
-    intervals:    it[0].num_intervals > 1
-    no_intervals: it[0].num_intervals <= 1
-}
-
-// Merge interval results
-vcf_to_merge = vcf_out.intervals
-    .map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ]}
-    .groupTuple()
-
-MERGE_VCFS(vcf_to_merge, dict)
-
-// Combine merged and non-interval results, clean up meta
-vcf_final = Channel.empty()
-    .mix(MERGE_VCFS.out.vcf, vcf_out.no_intervals)
-    .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'toolname' ], vcf ] }
-```
-
----
-
-## Configuration
-
-### Module Configuration Files
-
-Module behavior is controlled via `conf/modules/<tool>.config`:
-
-```groovy
-process {
-    withName: 'NEWTOOL_PROCESS' {
-        ext.args   = { params.newtool_args ?: '' }
-        ext.prefix = { "${meta.id}.newtool" }
-        publishDir = [
-            mode: params.publish_dir_mode,
-            path: { "${params.outdir}/variant_calling/newtool/${meta.id}/" },
-            pattern: "*{vcf.gz,vcf.gz.tbi}"
-        ]
-    }
-}
-```
-
-> **Note:** Older config files wrap process blocks in `if (params.tools && params.tools.split(',').contains('tool'))` guards. Do **not** use this pattern in new code — control which processes run via channel operations (`filter`, `branch`) in the workflow/subworkflow instead. When touching existing config files, remove these guards.
-
-### Resource Labels
-
-Use standard nf-core labels in `conf/base.config`:
-
-| Label                 | CPUs | Memory | Time |
-| --------------------- | ---- | ------ | ---- |
-| `process_single`      | 1    | 6 GB   | 8 h  |
-| `process_low`         | 2    | 12 GB  | 8 h  |
-| `process_medium`      | 6    | 36 GB  | 16 h |
-| `process_high`        | 12   | 72 GB  | 32 h |
-| `process_long`        | -    | -      | 40 h |
-| `process_high_memory` | -    | 200 GB | -    |
-
-### Adding New Parameters
-
-1. **Add to `nextflow.config`** with default value:
-
-   ```groovy
-   params {
-       new_param = false
-   }
-   ```
-
-2. **Update schema** using nf-core tools:
-
-   ```bash
-   nf-core pipelines schema build
-   ```
-
-3. **Add validation** if needed in the workflow
-
----
-
-## Testing
-
-### Test Framework
-
-Sarek uses **nf-test** for testing. Tests are in `tests/` directory.
-
-### Running Tests
-
-```bash
-# Run all tests
-nf-test test --profile debug,test,docker --verbose
-
-# Run specific test
-nf-test test tests/variant_calling_haplotypecaller.nf.test --profile debug,test,docker
-
-# Run with stub mode (faster, no actual execution)
-nf-test test tests/default.nf.test --profile debug,test,docker -stub
-
-# Update snapshots when outputs legitimately change
-nf-test test tests/my_test.nf.test --profile debug,test,docker --update-snapshot
-```
-
-### Test Structure with UTILS.groovy
-
-Tests use a scenario-based pattern:
-
-```groovy
-def projectDir = new File('.').absolutePath
-
-nextflow_pipeline {
-    name "Test pipeline"
-    script "../main.nf"
-    tag "pipeline"
-    tag "pipeline_sarek"
-
-    def test_scenario = [
-        [
-            name: "Test scenario name",
-            params: [
-                input: "${projectDir}/tests/csv/3.0/fastq_single.csv",
-                tools: 'haplotypecaller'
-            ]
-        ],
-        [
-            name: "Test with stub",
-            params: [],
-            stub: true
-        ],
-        [
-            name: "Fails with invalid input",
-            params: [
-                input: "${projectDir}/tests/csv/3.0/vcf_single.csv",
-                step: 'annotate',
-                vep_cache_version: 1,
-                build_only_index: true,
-                tools: 'vep'
-            ],
-            failure: true,
-            stdout: "Expected error message"
-        ]
-    ]
-
-    test_scenario.each { scenario ->
-        test(scenario.name, UTILS.get_test(scenario))
-    }
-}
-```
-
-### Test Scenario Options
-
-| Option                         | Description                               |
-| ------------------------------ | ----------------------------------------- |
-| `name`                         | Test name (descriptive)                   |
-| `params`                       | Map of parameters to set                  |
-| `stub`                         | Run in stub mode (boolean)                |
-| `failure`                      | Expect test to fail (boolean)             |
-| `stdout`                       | Expected stdout content for failure tests |
-| `gpu`                          | GPU test (adds gpu tag)                   |
-| `no_conda`                     | Incompatible with conda                   |
-| `include_muse_txt`             | Include MuSE txt in assertions            |
-| `include_freebayes_unfiltered` | Include freebayes unfiltered VCFs         |
-| `no_vcf_md5sum`                | Use VCF summary instead of md5            |
-
----
-
-## Documentation
-
-### Documentation Requirements
-
-**Any change that affects pipeline output or adds new functionality MUST include documentation updates.**
-
-### Documentation Files
-
-| File             | Purpose                  | When to Update                                  |
-| ---------------- | ------------------------ | ----------------------------------------------- |
-| `README.md`      | Pipeline overview        | **New tools** (add to overview/tool list)       |
-| `docs/usage.md`  | Usage instructions       | New parameters, new tools, input format changes |
-| `docs/output.md` | Output file descriptions | **Any change to outputs**, new tools            |
-| `CHANGELOG.md`   | Version history          | Every PR                                        |
-| `CITATIONS.md`   | Tool citations           | New tools                                       |
-| `docs/images/`   | Metro maps, diagrams     | **New tools**, workflow changes                 |
-
-### New Tool Documentation Checklist
-
-When adding a new tool, you **MUST** update ALL of the following:
-
-1. **`README.md`** - Add tool to the pipeline overview/feature list
-2. **`docs/usage.md`** - Document all new parameters and usage instructions
-3. **`docs/output.md`** - Document all output files produced by the tool
-4. **`docs/images/sarek_subway.*`** - Add tool to the metro map (SVG and PNG)
-5. **`CITATIONS.md`** - Add tool citation
-6. **`CHANGELOG.md`** - Document the addition
-
-### Output Changes Documentation
-
-Any PR that changes pipeline outputs (new files, changed file names, different content) **MUST** update:
-
-1. **`docs/output.md`** - Reflect the new/changed outputs
-2. **`CHANGELOG.md`** - Note the change under appropriate section
-
-### CHANGELOG Format
-
-Follow [Keep a Changelog](https://keepachangelog.com/) format.
-
-**Important conventions:**
-
-- Entries reference the **PR number**, not the issue number:
-  ```
-  - [#XXX](https://github.com/nf-core/sarek/pull/XXX) - Description of change
-  ```
-- Use `XXX` as placeholder when no PR exists yet
-- The issue number goes in the **PR description body** (for auto-close), not the changelog
-- Entries within each section are in **ascending order** by PR number
-
-```markdown
-## [Unreleased]
-
-### Added
-
-- [#PR_NUMBER](https://github.com/nf-core/sarek/pull/PR_NUMBER) - Description
-
-### Changed
-
-### Fixed
-
-### Removed
-
-### Dependencies
-
-| Dependency | Old version | New version |
-| ---------- | ----------- | ----------- |
-| tool_name  | 1.0.0       | 1.1.0       |
-
-### Parameters
-
-| Params        | status |
-| ------------- | ------ |
-| `--new_param` | New    |
-
-### Developer section
-
-#### Added
-
-#### Changed
-
-#### Fixed
-
-#### Removed
-```
-
-### Output Documentation
-
-In `docs/output.md`, document each tool's outputs:
-
-```markdown
-### Tool Name
-
-<details markdown="1">
-<summary>Output files</summary>
-
-- `path/to/output/`
-  - `*.extension`: Description of the file
-
-</details>
-
-Brief description of what this tool produces.
-```
-
----
-
-## Metro Map Updates
-
-### Metro Map Files
-
-Located in `docs/images/`:
-
-- `sarek_subway.svg` / `sarek_subway.png` - Main pipeline flow
-- `sarek_indices_subway.svg` / `sarek_indices_subway.png` - Index building flow
-
-### When to Update
-
-- Adding new tools or variant callers
-- Adding new preprocessing steps
-- Changing the pipeline flow
-- Adding new post-processing options
-
-### Update Process
-
-1. Edit the SVG file (use Inkscape or similar)
-2. Export to PNG
-3. Follow nf-core [design guidelines](https://nf-co.re/developers/design_guidelines)
-4. After release, checkout figures from `master` to `dev`:
-   ```bash
-   git checkout upstream/master -- docs/images/sarek_subway.svg
-   git checkout upstream/master -- docs/images/sarek_subway.png
-   ```
-
----
-
-## PR Checklist
-
-### Before Submitting
-
-- [ ] PR targets `dev` branch (not `master`)
-- [ ] Code follows Harshil alignment style
-- [ ] **Any touched code updated to strict syntax** (explicit closure params, underscore for unused vars)
-- [ ] **No new `ext.when` usage** - use channel operations instead
-- [ ] **Prefer channel operations** (`filter`, `branch`) over `if` statements for flow control
-- [ ] Pre-commit checks pass: `pre-commit run --all-files`
-- [ ] All tests pass: `nf-test test --profile debug,test,docker`
-- [ ] Linting passes: `nf-core pipelines lint`
-- [ ] No debug mode warnings
-
-### For New Tools
-
-**Code:**
-
-- [ ] Module added/imported correctly
-- [ ] Configuration in `conf/modules/<tool>.config`
-- [ ] Test added in `tests/`
-- [ ] MultiQC config updated (`assets/multiqc_config.yml`) if tool has MultiQC module
-
-**Documentation (ALL required):**
-
-- [ ] `README.md` - Tool added to pipeline overview/feature list
-- [ ] `docs/usage.md` - All parameters documented with usage instructions
-- [ ] `docs/output.md` - All output files documented
-- [ ] `docs/images/sarek_subway.svg` - Tool added to metro map
-- [ ] `docs/images/sarek_subway.png` - Exported PNG of updated metro map
-- [ ] `CITATIONS.md` - Tool citation added
-- [ ] `CHANGELOG.md` - Addition documented
-
-### For New Variant Callers
-
-Adding a variant caller touches **6 locations** — missing any of them causes silent bugs. All of the above "New Tools" items apply, plus:
-
-- [ ] **`nextflow_schema.json`** - Add to the `tools` parameter regex pattern
-- [ ] **Dispatcher subworkflow** - Add call and wire outputs into `vcf_all.mix(...)`:
-  - `subworkflows/local/bam_variant_calling_germline_all/main.nf` (germline callers)
-  - `subworkflows/local/bam_variant_calling_somatic_all/main.nf` (somatic pair callers)
-  - `subworkflows/local/bam_variant_calling_tumor_only_all/main.nf` (tumor-only callers)
-  - Use channel operations (`filter`, `branch`) to control execution — not `if` blocks (existing `if` blocks are legacy)
-- [ ] **`subworkflows/local/post_variantcalling/main.nf`** - Add to `small_variantcallers` list (for SNV callers eligible for normalization/filtering/consensus) or `excluded_variantcallers` (for SV callers). **Forgetting this silently excludes the caller from post-processing.**
-- [ ] **Individual subworkflow** - Set `variantcaller` in meta map (e.g., `meta + [variantcaller: 'toolname']`)
-
-### For New Parameters
-
-- [ ] Default value in `nextflow.config`
-- [ ] Schema updated: `nf-core pipelines schema build`
-- [ ] Validation added (if needed)
-- [ ] Documentation in `docs/usage.md`
-- [ ] CHANGELOG updated
-
-### For Changes Affecting Pipeline Output
-
-Any PR that changes output files (new files, renamed files, changed content):
-
-- [ ] `docs/output.md` updated to reflect changes
-- [ ] `CHANGELOG.md` updated
-- [ ] If significant workflow change: metro map updated (`docs/images/sarek_subway.*`)
-
----
-
-## Common Gotchas
-
-### 1. Forgetting `failOnDuplicate`/`failOnMismatch` on Joins
-
-**Problem:** Silent data loss or incorrect pairing
-**Solution:** Always use `join(..., failOnDuplicate: true, failOnMismatch: true)`
-
-### 2. Strelka Produces Two VCFs
-
-**Problem:** Strelka outputs SNV and Indel VCFs separately
-**Solution:** Use `groupTuple(size: 2)` then `BCFTOOLS_CONCAT` before downstream processing
-
-### 3. Blocking GroupTuple
-
-**Problem:** `groupTuple()` without size blocks pipeline
-**Solution:** Use `groupKey(meta, meta.num_intervals)` when size is known
-
-### 4. Meta Fields Persisting
-
-**Problem:** Temporary meta fields (like `num_intervals`) persist in output
-**Solution:** Clean up with `meta - meta.subMap('field_name')` before emit
-
-### 5. DeepVariant Conda
-
-**Problem:** DeepVariant doesn't support Conda
-**Solution:** Note in module: `// FIXME Conda is not supported at the moment`
-
-### 6. BWA Memory Requirements
-
-**Problem:** Unexpected OOM errors
-**Solution:** BWA requires ~5.37N memory, BWA-MEM2 requires ~28N GB where N = reference size
-
-### 7. Using Deprecated `ext.when` Pattern
-
-**Problem:** Old code uses `ext.when` in config to control module execution
-**Solution:** When touching this code, refactor to use channel operations (`filter`, `branch`) to control dataflow. Nextflow is a dataflow language - let the data drive execution. Avoid both `ext.when` AND `if` statements where possible.
-
-### 8. Forgetting to Register a New Variant Caller
-
-**Problem:** New variant caller runs and produces VCFs, but is silently excluded from normalization, filtering, and consensus calling
-**Solution:** Must update all 6 registration points — see [For New Variant Callers](#for-new-variant-callers) checklist. The most commonly missed is `post_variantcalling/main.nf`'s `small_variantcallers` list.
-
-### 9. Implicit Variables in Closures
-
-**Problem:** Using implicit `it` makes code harder to read and review
-**Solution:** Always use explicit named parameters in closures: `.map { meta, vcf -> ... }` not `.map { it[0], it[1] -> ... }`
-
----
-
-## Quick Reference
-
-### Essential Commands
-
-```bash
-# Run tests
-nf-test test --profile debug,test,docker
-
-# Lint pipeline
-nf-core pipelines lint
-
-# Update schema
-nf-core pipelines schema build
-
-# Install/update module
-nf-core modules install <tool>/<subcommand>
-nf-core modules update <tool>/<subcommand>
-```
-
-### Key Files for Common Changes
-
-| Change Type   | Primary Files                                   |
-| ------------- | ----------------------------------------------- |
-| New parameter | `nextflow.config`, `nextflow_schema.json`       |
-| New tool      | `conf/modules/<tool>.config`, subworkflow, test |
-| Bug fix       | Relevant module/subworkflow, test               |
-| Documentation | `docs/usage.md`, `docs/output.md`               |
-| Any change    | `CHANGELOG.md`                                  |
-
----
-
-## Getting Help
-
-- **Slack:** [#sarek channel](https://nfcore.slack.com/channels/sarek)
-- **GitHub Issues:** [nf-core/sarek/issues](https://github.com/nf-core/sarek/issues)
-- **Documentation:** [nf-co.re/sarek](https://nf-co.re/sarek)

From de19184f59f8e74b2037d3e6bcf243f0a56f1390 Mon Sep 17 00:00:00 2001
From: apolitics <55941893+apolitics@users.noreply.github.com>
Date: Tue, 14 Jul 2026 15:28:25 +0300
Subject: [PATCH 06/27] =?UTF-8?q?Fix=20`--normalize=5Fvcfs`=20dropping=20a?=
 =?UTF-8?q?n=20allele=20of=201/2=20multiallelic=20sites=20(`--rm-dup=20all?=
 =?UTF-8?q?`=20=E2=86=92=20`exact`)=20(#2216)?=
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Closes #2215.

When `--normalize_vcfs` is enabled, `VCFS_NORM` runs `bcftools norm
--multiallelics -both --rm-dup all`. Because `-m -both` splits a
multiallelic record into per-allele rows **at the same position**, the
subsequent `--rm-dup all` (which de-duplicates **by position**) deletes
all but the first row, **silently dropping a real ALT allele** of
heterozygous two-alt (`1/2`) sites.

This switches the argument to `--rm-dup exact`, which still removes
truly identical duplicate records (the stated intent of the existing
comment) but no longer collapses two distinct alleles that share a
position.

## Evidence

Test profile, `chr22:13575` (DeepVariant `G → C,T`, GT `1/2`):

| `bcftools norm` args | records | site 13575 |
|---|---|---|
| `-m -both --rm-dup all` (before) | 17 | only `G→C` — `G→T` lost |
| `-m -both --rm-dup exact` (after) | 18 | `G→C` and `G→T` |

Verified end-to-end by re-running `-profile test,docker --tools
deepvariant --normalize_vcfs --filter_vcfs` with the change: the
normalized VCF keeps both alleles.

## PR checklist

- [x] Minimal, focused change (one config argument + CHANGELOG)
- [x] Branched off `dev`, targets `dev`
- [x] `CHANGELOG.md` updated
- [ ] CI green

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
---
 CHANGELOG.md                             | 2 ++
 conf/modules/post_variant_calling.config | 2 +-
 2 files changed, 3 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 175d6dc100..c00d1f3fab 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,6 +17,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Fixed
 
+- [#2216](https://github.com/nf-core/sarek/pull/2216) - Fix `--normalize_vcfs` dropping a real ALT allele of `1/2` multiallelic sites (`bcftools norm --rm-dup all` → `--rm-dup exact`)
+
 ### Removed
 
 ### Dependencies - modules
diff --git a/conf/modules/post_variant_calling.config b/conf/modules/post_variant_calling.config
index 2eb80cd0dd..a42cefd668 100644
--- a/conf/modules/post_variant_calling.config
+++ b/conf/modules/post_variant_calling.config
@@ -41,7 +41,7 @@ process {
         ext.prefix = { vcf.baseName - '.added_info.vcf' + '.norm' }
         ext.args = { [
             '--multiallelics -both', // split multiallelic sites into biallelic records and both SNPs and indels should be merged separately into two records
-            '--rm-dup all'           // output only the first instance of a record which is present multiple times
+            '--rm-dup exact'         // output only the first instance of a record which is present multiple times
         ].join(' ') }
         publishDir = [
             enabled: false

From 41a8e7059a7b070dabbc1575054af397b15a19ed Mon Sep 17 00:00:00 2001
From: Jonathan Manning <jonathan.manning@seqera.io>
Date: Wed, 15 Jul 2026 11:11:40 +0100
Subject: [PATCH 07/27] Skip nf-schema path validation for annotation_cache and
 igenomes_base (#2184)

## Summary

`snpeff_cache`, `vep_cache` and `igenomes_base` are declared in the
schema with `format: directory-path` and default to S3 buckets.
nf-schema 2.5+ resolves these defaults against the storage backend and
fails the launch if the path is unreachable - no credentials, no
network, or an SSO/IAM session that can't see the bucket. Any user
without read access to `s3://annotation-cache/` (e.g. on-prem clusters,
restricted cloud accounts) currently can't launch the pipeline at all,
even when they have no intention of running snpEff or VEP.

## Details

The full debate of where this fix belongs is in
[nextflow-io/nf-schema#204](https://github.com/nextflow-io/nf-schema/issues/204).
The plugin maintainer's settled position is that:

1. The validator should fail when a configured path is unreachable -
that's the point.
2. The right fix for default-only S3 paths that nobody explicitly opted
into is to:
- drop `format: "directory-path"` so the existence probe never runs, and
- add the param to `validation.defaultIgnoreParams` so nf-schema skips
it entirely.

That guidance has been applied in nf-core/rnaseq for `igenomes_base`
(PRs [#1696](https://github.com/nf-core/rnaseq/pull/1696) and
[#1739](https://github.com/nf-core/rnaseq/pull/1739)). This PR applies
the same treatment to sarek's three S3-defaulted directory params.

PR [#2083](https://github.com/nf-core/sarek/pull/2083) already removed
`exists: true` from `snpeff_cache`/`vep_cache`, but `format:
directory-path` alone is enough to trigger the existence check, so
launches still fail for users without access to the default bucket.

Fixes #2079

## Changes

- `nextflow.config`: add `igenomes_base`, `snpeff_cache`, `vep_cache` to
`validation.defaultIgnoreParams`.
- `nextflow_schema.json`: drop `"format": "directory-path"` from the
same three params. Derived/resolved paths are still validated
individually downstream.

## Test plan

- [ ] Launch from an environment without read access to
`s3://annotation-cache/` - validation should now pass.
- [ ] Launch with valid `--snpeff_cache`/`--vep_cache` overrides -
annotation should still work.
- [ ] Launch with `--tools snpeff,vep` and confirm download-cache flows
still error appropriately if the path is bad at the point of use (not at
schema validation).

---------

Co-authored-by: Claude Opus 4.7 (1M context) <noreply@anthropic.com>
Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
Co-authored-by: Friederike Hanssen <friederike.hanssen@seqera.io>
---
 CHANGELOG.md         | 1 +
 nextflow.config      | 2 +-
 nextflow_schema.json | 3 ---
 3 files changed, 2 insertions(+), 4 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index c00d1f3fab..3f5691ffe8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -17,6 +17,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Fixed
 
+- [#2184](https://github.com/nf-core/sarek/pull/2184) - Skip nf-schema path-existence validation for `snpeff_cache`, `vep_cache` and `igenomes_base` so pipeline launches succeed when the default S3 buckets are not accessible
 - [#2216](https://github.com/nf-core/sarek/pull/2216) - Fix `--normalize_vcfs` dropping a real ALT allele of `1/2` multiallelic sites (`bcftools norm --rm-dup all` → `--rm-dup exact`)
 
 ### Removed
diff --git a/nextflow.config b/nextflow.config
index 4a9b5bcd74..e29cdd1f5e 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -779,7 +779,7 @@ plugins {
 }
 
 validation {
-    defaultIgnoreParams = ["genomes", "freebayes_filter", "vep_cache_version"]
+    defaultIgnoreParams = ["genomes", "freebayes_filter", "vep_cache_version", "igenomes_base", "snpeff_cache", "vep_cache"]
     monochromeLogs = params.monochrome_logs
 }
 
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 19ef1af732..07493019c4 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -842,7 +842,6 @@
             "properties": {
                 "igenomes_base": {
                     "type": "string",
-                    "format": "directory-path",
                     "description": "The base path to the igenomes reference files",
                     "fa_icon": "fas fa-ban",
                     "default": "s3://ngi-igenomes/igenomes/"
@@ -1123,7 +1122,6 @@
                 },
                 "snpeff_cache": {
                     "type": "string",
-                    "format": "directory-path",
                     "fa_icon": "fas fa-cloud-download-alt",
                     "default": "s3://annotation-cache/snpeff_cache/",
                     "description": "Path to snpEff cache.",
@@ -1137,7 +1135,6 @@
                 },
                 "vep_cache": {
                     "type": "string",
-                    "format": "directory-path",
                     "fa_icon": "fas fa-cloud-download-alt",
                     "default": "s3://annotation-cache/vep_cache/",
                     "description": "Path to VEP cache.",

From a0d5fd30e88e132d4f8e2f62c3fb560f5e8d8b06 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Famke=20B=C3=A4uerle?=
 <45968370+famosab@users.noreply.github.com>
Date: Wed, 15 Jul 2026 14:40:25 +0200
Subject: [PATCH 08/27] Update bcftools to 1.23.1 (#2210)

<!--
# nf-core/sarek pull request

Many thanks for contributing to nf-core/sarek!

Please fill in the appropriate checklist below (delete whatever is not
relevant).
These are the most common things requested on pull requests (PRs).

Remember that PRs should be made against the dev branch, unless you're
preparing a pipeline release.

Learn more about contributing:
[CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
-->

## PR checklist

- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [ ] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [ ] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).

---------

Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
Co-authored-by: Friederike Hanssen <friederike.hanssen@seqera.io>
Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |  3 +
 main.nf                                       |  2 +-
 modules.json                                  | 22 ++---
 .../nf-core/bcftools/annotate/environment.yml |  5 +-
 modules/nf-core/bcftools/annotate/main.nf     | 63 ++++++-------
 modules/nf-core/bcftools/annotate/meta.yml    | 80 +++++++++--------
 .../nf-core/bcftools/concat/environment.yml   |  5 +-
 modules/nf-core/bcftools/concat/main.nf       | 78 ++++++++--------
 modules/nf-core/bcftools/concat/meta.yml      | 58 ++++++------
 modules/nf-core/bcftools/isec/environment.yml |  5 +-
 modules/nf-core/bcftools/isec/main.nf         | 40 ++++-----
 modules/nf-core/bcftools/isec/meta.yml        | 64 ++++++++++---
 .../nf-core/bcftools/merge/environment.yml    |  6 +-
 modules/nf-core/bcftools/merge/main.nf        | 81 +++++++++--------
 modules/nf-core/bcftools/merge/meta.yml       | 49 +++++-----
 .../nf-core/bcftools/mpileup/environment.yml  |  4 +-
 modules/nf-core/bcftools/mpileup/main.nf      | 49 +++++-----
 modules/nf-core/bcftools/mpileup/meta.yml     | 81 +++++++++++------
 modules/nf-core/bcftools/norm/environment.yml |  5 +-
 modules/nf-core/bcftools/norm/main.nf         | 62 ++++++-------
 modules/nf-core/bcftools/norm/meta.yml        | 62 +++++++------
 modules/nf-core/bcftools/sort/environment.yml |  5 +-
 modules/nf-core/bcftools/sort/main.nf         | 69 +++++++-------
 modules/nf-core/bcftools/sort/meta.yml        | 55 +++++++-----
 .../nf-core/bcftools/stats/environment.yml    |  6 +-
 modules/nf-core/bcftools/stats/main.nf        | 40 ++++-----
 modules/nf-core/bcftools/stats/meta.yml       | 41 +++++++--
 modules/nf-core/bcftools/view/environment.yml |  6 +-
 modules/nf-core/bcftools/view/main.nf         | 76 ++++++++--------
 modules/nf-core/bcftools/view/meta.yml        | 53 +++++------
 .../nf-core/ngscheckmate/ncm/environment.yml  |  2 +-
 modules/nf-core/ngscheckmate/ncm/main.nf      | 44 ++++-----
 modules/nf-core/ngscheckmate/ncm/meta.yml     | 89 +++++++++++++------
 .../bam_variant_calling_freebayes/main.nf     |  1 -
 .../bam_variant_calling_germline_all/main.nf  |  1 +
 .../local/bam_variant_calling_mpileup/main.nf | 13 ++-
 .../bam_variant_calling_somatic_all/main.nf   |  2 +
 .../main.nf                                   |  1 +
 subworkflows/local/cram_sampleqc/main.nf      | 12 ++-
 .../local/post_variantcalling/main.nf         |  5 +-
 subworkflows/local/prepare_genome/main.nf     |  2 +-
 subworkflows/local/vcf_annotate_all/main.nf   |  8 +-
 .../local/vcf_concatenate_germline/main.nf    |  4 +-
 subworkflows/local/vcf_consensus/main.nf      |  8 +-
 subworkflows/local/vcf_normalization/main.nf  |  6 +-
 .../local/vcf_qc_bcftools_vcftools/main.nf    |  1 -
 .../local/vcf_varlociraptor_single/main.nf    | 12 +--
 .../local/vcf_varlociraptor_somatic/main.nf   | 19 ++--
 subworkflows/nf-core/bam_ngscheckmate/main.nf | 19 ++--
 .../nf-core/bam_ngscheckmate/meta.yml         |  4 +
 tests/aligner-parabricks.nf.test.snap         |  4 +-
 tests/annotation_bcfann.nf.test.snap          |  4 +-
 tests/bbsplit.nf.test.snap                    |  6 +-
 tests/default.nf.test.snap                    |  6 +-
 ...joint_calling_haplotypecaller.nf.test.snap | 14 ++-
 tests/joint_calling_mutect2.nf.test.snap      |  8 +-
 tests/postprocess_concatenation.nf.test.snap  | 16 ++--
 ...s_concatenation_normalization.nf.test.snap | 44 ++++-----
 tests/postprocess_consensus.nf.test.snap      | 78 ++++++++--------
 tests/postprocess_filtering.nf.test.snap      | 14 +--
 tests/postprocess_normalization.nf.test.snap  | 18 ++--
 tests/postprocess_varlociraptor.nf.test.snap  | 39 ++++----
 tests/qc_ngscheckmate.nf.test.snap            |  4 +-
 tests/save_output_as_bam.nf.test.snap         |  4 +-
 ...art_from_preparerecalibration.nf.test.snap |  8 +-
 tests/start_from_recalibration.nf.test.snap   |  8 +-
 tests/tumor-normal-pair.nf.test.snap          |  8 +-
 tests/umi_in_read_names.nf.test.snap          |  8 +-
 tests/variant_calling_all.nf.test.snap        | 54 +++++------
 .../variant_calling_deepvariant.nf.test.snap  | 12 +--
 tests/variant_calling_freebayes.nf.test.snap  | 40 ++++-----
 ...riant_calling_haplotypecaller.nf.test.snap | 16 ++--
 tests/variant_calling_lofreq.nf.test.snap     |  8 +-
 tests/variant_calling_manta.nf.test.snap      | 42 ++++-----
 tests/variant_calling_mpileup.nf.test.snap    | 24 ++---
 tests/variant_calling_muse.nf.test.snap       |  6 +-
 tests/variant_calling_mutect2.nf.test.snap    | 16 ++--
 ...ant_calling_sentieon_dnascope.nf.test.snap | 15 ++--
 ...ling_sentieon_haplotypecaller.nf.test.snap | 19 ++--
 tests/variant_calling_strelka.nf.test.snap    | 32 +++----
 tests/variant_calling_strelka_bp.nf.test.snap | 28 +++---
 tests/variant_calling_tiddit.nf.test.snap     | 14 +--
 workflows/sarek.nf                            |  2 +-
 83 files changed, 1091 insertions(+), 956 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3f5691ffe8..118d50347a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
+- [#2210](https://github.com/nf-core/sarek/pull/2210) - Update bcftools to 1.23.1
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update EnsemblVEP to 116.0
 
 ### Fixed
@@ -26,6 +27,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 | Dependency    | Old version | New version |
 | ------------- | ----------- | ----------- |
+| bcftools      | 1.21        | 1.23.1      |
+| htslib        | 1.21        | 1.23.1      |
 | varlociraptor | 8.9.3       | 8.9.5       |
 | ensembl-vep   | 115.2       | 116.0       |
 
diff --git a/main.nf b/main.nf
index 7506eec94a..c03cc52266 100755
--- a/main.nf
+++ b/main.nf
@@ -289,7 +289,7 @@ workflow NFCORE_SAREK {
         PREPARE_GENOME.out.bbsplit_index,
         PREPARE_GENOME.out.bcftools_annotations,
         PREPARE_GENOME.out.bcftools_annotations_tbi,
-        params.bcftools_columns ? channel.fromPath(params.bcftools_columns).collect() : channel.value([]),
+        params.bcftools_columns ? channel.fromPath(params.bcftools_columns).collect() : channel.value([[]]),
         params.bcftools_header_lines ? channel.fromPath(params.bcftools_header_lines).collect() : channel.empty(),
         params.cf_chrom_len ? channel.fromPath(params.cf_chrom_len).collect() : [],
         PREPARE_GENOME.out.chr_dir,
diff --git a/modules.json b/modules.json
index d2272c9181..bc9f66c29c 100644
--- a/modules.json
+++ b/modules.json
@@ -17,47 +17,47 @@
                     },
                     "bcftools/annotate": {
                         "branch": "master",
-                        "git_sha": "28438d38e50710e20885ff2594344b9d5a03dec7",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/concat": {
                         "branch": "master",
-                        "git_sha": "1503efe8f6450e71218097f93cf43e4b625018d4",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/isec": {
                         "branch": "master",
-                        "git_sha": "f17049e03697726ace7499d2fe342f892594f6f3",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/merge": {
                         "branch": "master",
-                        "git_sha": "f17049e03697726ace7499d2fe342f892594f6f3",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/mpileup": {
                         "branch": "master",
-                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["bam_ngscheckmate"]
                     },
                     "bcftools/norm": {
                         "branch": "master",
-                        "git_sha": "39fed2e840a805454a64dda9c2ef64c00e2c6781",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/sort": {
                         "branch": "master",
-                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/stats": {
                         "branch": "master",
-                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bcftools/view": {
                         "branch": "master",
-                        "git_sha": "f17049e03697726ace7499d2fe342f892594f6f3",
+                        "git_sha": "feef37435aea56816adf4b3bde1fc76aac327a8d",
                         "installed_by": ["modules"]
                     },
                     "bwa/index": {
@@ -403,7 +403,7 @@
                     },
                     "ngscheckmate/ncm": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["bam_ngscheckmate"]
                     },
                     "parabricks/fq2bam": {
@@ -612,7 +612,7 @@
                 "nf-core": {
                     "bam_ngscheckmate": {
                         "branch": "master",
-                        "git_sha": "c9c3ef86c1892413b3c86fb38c4e39fd7288512f",
+                        "git_sha": "3d9c2f4beaa4f62b3f006928fd9095a496d1e5a8",
                         "installed_by": ["subworkflows"]
                     },
                     "cache_download_ensemblvep_snpeff": {
diff --git a/modules/nf-core/bcftools/annotate/environment.yml b/modules/nf-core/bcftools/annotate/environment.yml
index 557488607c..13b63a623e 100644
--- a/modules/nf-core/bcftools/annotate/environment.yml
+++ b/modules/nf-core/bcftools/annotate/environment.yml
@@ -4,4 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
+  # renovate: datasource=conda depName=bioconda/bcftools
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/annotate/main.nf b/modules/nf-core/bcftools/annotate/main.nf
index 095643369c..f291e3c291 100644
--- a/modules/nf-core/bcftools/annotate/main.nf
+++ b/modules/nf-core/bcftools/annotate/main.nf
@@ -3,36 +3,35 @@ process BCFTOOLS_ANNOTATE {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data'
-        : 'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
-    tuple val(meta), path(input), path(index), path(annotations), path(annotations_index)
-    path columns
-    path header_lines
-    path rename_chrs
+    tuple val(meta), path(input), path(index), path(annotations), path(annotations_index), path(columns), path(header_lines), path(rename_chrs)
 
     output:
-    tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
-    tuple val(meta), path("*.tbi"),                     emit: tbi, optional: true
-    tuple val(meta), path("*.csi"),                     emit: csi, optional: true
-    path "versions.yml",                                emit: versions
+    tuple val(meta), path("${prefix}.${extension}"), emit: vcf
+    tuple val(meta), path("${prefix}.${extension}.{tbi,csi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     def annotations_file = annotations ? "--annotations ${annotations}" : ''
     def columns_file = columns ? "--columns-file ${columns}" : ''
     def header_file = header_lines ? "--header-lines ${header_lines}" : ''
     def rename_chrs_file = rename_chrs ? "--rename-chrs ${rename_chrs}" : ''
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf"
+    extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf"
     def index_command = !index ? "bcftools index ${input}" : ''
 
     if ("${input}" == "${prefix}.${extension}") {
@@ -51,24 +50,23 @@ process BCFTOOLS_ANNOTATE {
         --output ${prefix}.${extension} \\
         --threads ${task.cpus} \\
         ${input}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' )
-    END_VERSIONS
     """
 
     stub:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-    def index_extension = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                            args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                            args.contains("--write-index") || args.contains("-W") ? "csi" : ""
+    prefix = task.ext.prefix ?: "${meta.id}"
+    extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf"
+    def index_extension = args.contains("--write-index=tbi") || args.contains("-W=tbi")
+        ? "tbi"
+        : args.contains("--write-index=csi") || args.contains("-W=csi")
+            ? "csi"
+            : args.contains("--write-index") || args.contains("-W") ? "csi" : ""
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = extension.endsWith(".gz") && index_extension.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index_extension}" : ""
 
@@ -78,10 +76,5 @@ process BCFTOOLS_ANNOTATE {
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/annotate/meta.yml b/modules/nf-core/bcftools/annotate/meta.yml
index 058954d21d..066e8544f2 100644
--- a/modules/nf-core/bcftools/annotate/meta.yml
+++ b/modules/nf-core/bcftools/annotate/meta.yml
@@ -12,7 +12,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: https://samtools.github.io/bcftools/bcftools.html#annotate
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -22,7 +23,8 @@ input:
           e.g. [ id:'test', single_end:false ]
     - input:
         type: file
-        description: Query VCF or BCF file, can be either uncompressed or compressed
+        description: Query VCF or BCF file, can be either uncompressed or
+          compressed
         ontologies: []
     - index:
         type: file
@@ -36,19 +38,20 @@ input:
         type: file
         description: Index of the annotations file
         ontologies: []
-  - columns:
-      type: file
-      description: List of columns in the annotations file, one name per row
-      ontologies: []
-  - header_lines:
-      type: file
-      description: Contains lines to append to the output VCF header
-      ontologies: []
-  - rename_chrs:
-      type: file
-      description: Rename annotations according to this file containing "old_name new_name\n"
-        pairs separated by whitespaces, each on a separate line.
-      ontologies: []
+    - columns:
+        type: file
+        description: List of columns in the annotations file, one name per row
+        ontologies: []
+    - header_lines:
+        type: file
+        description: Contains lines to append to the output VCF header
+        ontologies: []
+    - rename_chrs:
+        type: file
+        description: Rename annotations according to this file containing
+          "old_name new_name\n" pairs separated by whitespaces, each on a separate
+          line.
+        ontologies: []
 output:
   vcf:
     - - meta:
@@ -56,40 +59,43 @@ output:
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.{vcf,vcf.gz,bcf,bcf.gz}":
+      - ${prefix}.${extension}:
           type: file
           description: Compressed annotated VCF file
           pattern: "*{vcf,vcf.gz,bcf,bcf.gz}"
           ontologies: []
-  tbi:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
-          type: file
-          description: Alternative VCF file index
-          pattern: "*.tbi"
-          ontologies: []
-  csi:
+  index:
     - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.csi":
+      - "${prefix}.${extension}.{tbi,csi}":
           type: file
-          description: Default VCF file index
-          pattern: "*.csi"
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
           ontologies: []
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@projectoriented"
   - "@ramprasadn"
diff --git a/modules/nf-core/bcftools/concat/environment.yml b/modules/nf-core/bcftools/concat/environment.yml
index 557488607c..13b63a623e 100644
--- a/modules/nf-core/bcftools/concat/environment.yml
+++ b/modules/nf-core/bcftools/concat/environment.yml
@@ -4,4 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
+  # renovate: datasource=conda depName=bioconda/bcftools
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/concat/main.nf b/modules/nf-core/bcftools/concat/main.nf
index 7f6874e553..f68bd98cc5 100644
--- a/modules/nf-core/bcftools/concat/main.nf
+++ b/modules/nf-core/bcftools/concat/main.nf
@@ -1,72 +1,72 @@
 process BCFTOOLS_CONCAT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
     tuple val(meta), path(vcfs), path(tbi)
 
     output:
-    tuple val(meta), path("${prefix}.${extension}")    , emit: vcf
-    tuple val(meta), path("${prefix}.${extension}.tbi"), emit: tbi, optional: true
-    tuple val(meta), path("${prefix}.${extension}.csi"), emit: csi, optional: true
-    path  "versions.yml"                               , emit: versions
+    tuple val(meta), path("${prefix}.${extension}"), emit: vcf
+    tuple val(meta), path("${prefix}.${extension}.{tbi,csi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
     def tbi_names = tbi.findAll { file -> !(file instanceof List) }.collect { file -> file.name }
     def create_input_index = vcfs.collect { vcf -> tbi_names.contains(vcf.name + ".tbi") || tbi_names.contains(vcf.name + ".csi") ? "" : "tabix ${vcf}" }.join("\n    ")
-    extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                "vcf"
-    def input = vcfs.sort{it.toString()}.join(" ")
+    extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def input = vcfs.sort { vcf -> vcf.toString() }.join(" ")
     """
     ${create_input_index}
 
     bcftools concat \\
         --output ${prefix}.${extension} \\
-        $args \\
-        --threads $task.cpus \\
+        ${args} \\
+        --threads ${task.cpus} \\
         ${input}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
-    extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                "vcf"
-    def index_extension = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                        args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                        args.contains("--write-index") || args.contains("-W") ? "csi" :
-                        ""
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def index_extension = args.contains("--write-index=tbi") || args.contains("-W=tbi")
+        ? "tbi"
+        : args.contains("--write-index=csi") || args.contains("-W=csi")
+            ? "csi"
+            : args.contains("--write-index") || args.contains("-W")
+                ? "csi"
+                : ""
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = extension.endsWith(".gz") && index_extension.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index_extension}" : ""
 
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/concat/meta.yml b/modules/nf-core/bcftools/concat/meta.yml
index 3d12673a29..6fd768b8f3 100644
--- a/modules/nf-core/bcftools/concat/meta.yml
+++ b/modules/nf-core/bcftools/concat/meta.yml
@@ -12,7 +12,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -31,8 +32,8 @@ input:
           List containing 2 or more index files (optional)
           e.g. [ 'file1.tbi', 'file2.tbi' ]
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -43,35 +44,38 @@ output:
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
           pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}"
-  - tbi:
-      - meta:
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - ${prefix}.${extension}.tbi:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-          pattern: "*.tbi"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - ${prefix}.${extension}.csi:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-          pattern: "*.csi"
-  - versions:
-      - versions.yml:
+      - "${prefix}.${extension}.{tbi,csi}":
           type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
+          ontologies: []
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@abhi18av"
   - "@nvnieuwk"
diff --git a/modules/nf-core/bcftools/isec/environment.yml b/modules/nf-core/bcftools/isec/environment.yml
index ba863b388f..ee5cb56dad 100644
--- a/modules/nf-core/bcftools/isec/environment.yml
+++ b/modules/nf-core/bcftools/isec/environment.yml
@@ -5,6 +5,5 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.22.1
-  # renovate: datasource=conda depName=bioconda/bcftools
-  - bioconda::bcftools=1.22
+  - bioconda::bcftools=1.23.1
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/isec/main.nf b/modules/nf-core/bcftools/isec/main.nf
index c9bdf4ce34..d8a3fb23e8 100644
--- a/modules/nf-core/bcftools/isec/main.nf
+++ b/modules/nf-core/bcftools/isec/main.nf
@@ -1,39 +1,40 @@
 process BCFTOOLS_ISEC {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/474a5ea8dc03366b04df884d89aeacc4f8e6d1ad92266888e7a8e7958d07cde8/data':
-        'community.wave.seqera.io/library/bcftools_htslib:0a3fa2654b52006f' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
-    tuple val(meta), path(vcfs), path(tbis)
+    tuple val(meta), path(vcfs), path(tbis), path(file_list), path(targets_file), path(regions_file)
 
     output:
     tuple val(meta), path("${prefix}", type: "dir"), emit: results
-    path  "versions.yml"                           , emit: versions
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    targets_file_args = targets_file ? "-T ${targets_file}" : ''
+    regions_file_args = regions_file ? "-R ${regions_file}" : ''
+    vcf_files = file_list ? "-l ${file_list}" : "${vcfs}"
+
     """
     bcftools isec  \\
-        $args \\
-        -p $prefix \\
-        ${vcfs}
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
+        ${args} \\
+        ${targets_file_args} \\
+        ${regions_file_args} \\
+        -p ${prefix} \\
+        ${vcf_files} \\
     """
 
     stub:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     mkdir ${prefix}
     touch ${prefix}/README.txt
@@ -42,10 +43,5 @@ process BCFTOOLS_ISEC {
     touch ${prefix}/0000.vcf.gz.tbi
     echo "" | gzip > ${prefix}/0001.vcf.gz
     touch ${prefix}/0001.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/isec/meta.yml b/modules/nf-core/bcftools/isec/meta.yml
index 55cb49f4c7..82938fb902 100644
--- a/modules/nf-core/bcftools/isec/meta.yml
+++ b/modules/nf-core/bcftools/isec/meta.yml
@@ -14,7 +14,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -27,11 +28,37 @@ input:
         description: |
           List containing 2 or more vcf/bcf files. These must be compressed and have an associated index.
           e.g. [ 'file1.vcf.gz', 'file2.vcf' ]
+        pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}"
+        ontologies:
+          - edam: "http://edamontology.org/format_3016"
+          - edam: "http://edamontology.org/format_3570"
     - tbis:
         type: list
         description: |
           List containing the tbi index files corresponding to the vcf/bcf input files
-          e.g. [ 'file1.vcf.tbi', 'file2.vcf.tbi' ]
+        pattern: "*.tbi"
+        ontologies:
+          - edam: "http://edamontology.org/format_3475"
+    - file_list:
+        type: file
+        description: |
+          Optional text file containing the list of VCF/BCF files to be processed by bcftools isec, one per line.
+        ontologies:
+          - edam: "http://edamontology.org/format_2330"
+    - targets_file:
+        type: file
+        description: |
+          Optional file containing target regions to restrict the analysis to.
+        ontologies:
+          - edam: "http://edamontology.org/format_3003"
+          - edam: "http://edamontology.org/format_3475"
+    - regions_file:
+        type: file
+        description: |
+          Optional file containing regions to restrict the analysis to.
+        ontologies:
+          - edam: "http://edamontology.org/format_3003"
+          - edam: "http://edamontology.org/format_3475"
 output:
   results:
     - - meta:
@@ -41,15 +68,32 @@ output:
             e.g. [ id:'test', single_end:false ]
       - ${prefix}:
           type: directory
-          description: Folder containing the set operations results perform on the vcf files
-          pattern: "${prefix}"
+          description: Directory containing the output files from bcftools isec
+          pattern: "${prefix}/"
+          ontologies:
+            - edam: "http://edamontology.org/format_3016"
+            - edam: "http://edamontology.org/format_3570"
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/bcftools/merge/environment.yml b/modules/nf-core/bcftools/merge/environment.yml
index ba863b388f..13b63a623e 100644
--- a/modules/nf-core/bcftools/merge/environment.yml
+++ b/modules/nf-core/bcftools/merge/environment.yml
@@ -4,7 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/bcftools
-  - bioconda::bcftools=1.22
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/merge/main.nf b/modules/nf-core/bcftools/merge/main.nf
index c560a90212..215456ee8c 100644
--- a/modules/nf-core/bcftools/merge/main.nf
+++ b/modules/nf-core/bcftools/merge/main.nf
@@ -1,22 +1,20 @@
 process BCFTOOLS_MERGE {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/474a5ea8dc03366b04df884d89aeacc4f8e6d1ad92266888e7a8e7958d07cde8/data':
-        'community.wave.seqera.io/library/bcftools_htslib:0a3fa2654b52006f' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
-    tuple val(meta), path(vcfs), path(tbis)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
-    tuple val(meta4), path(bed)
+    tuple val(meta), path(vcfs), path(tbis), path(bed)
+    tuple val(meta2), path(fasta), path(fai)
 
     output:
     tuple val(meta), path("*.{bcf,vcf}{,.gz}"), emit: vcf
-    tuple val(meta), path("*.{csi,tbi}")      , emit: index, optional: true
-    path "versions.yml"                       , emit: versions
+    tuple val(meta), path("*.{csi,tbi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,50 +23,51 @@ process BCFTOOLS_MERGE {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
 
-    def input = (vcfs.collect().size() > 1) ? vcfs.sort{ it.name } : vcfs
-    def regions = bed ? "--regions-file $bed" : ""
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
+    def input = vcfs.collect().size() > 1 ? vcfs.sort { vcf -> vcf.name } : vcfs
+    def regions = bed ? "--regions-file ${bed}" : ""
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
 
     """
     bcftools merge \\
-        $args \\
-        $regions \\
-        --threads $task.cpus \\
+        ${args} \\
+        ${regions} \\
+        --threads ${task.cpus} \\
         --output ${prefix}.${extension} \\
-        $input
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
+        ${input}
     """
 
     stub:
-    def args = task.ext.args   ?: ''
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                args.contains("--write-index") || args.contains("-W") ? "csi" :
-                ""
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi")
+        ? "tbi"
+        : args.contains("--write-index=csi") || args.contains("-W=csi")
+            ? "csi"
+            : args.contains("--write-index") || args.contains("-W")
+                ? "csi"
+                : ""
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : ""
 
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/merge/meta.yml b/modules/nf-core/bcftools/merge/meta.yml
index 09af245a2c..d1f14ba5fc 100644
--- a/modules/nf-core/bcftools/merge/meta.yml
+++ b/modules/nf-core/bcftools/merge/meta.yml
@@ -11,7 +11,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -31,6 +32,11 @@ input:
           List containing the tbi index files corresponding to the vcfs input files
           e.g. [ 'file1.vcf.tbi', 'file2.vcf.tbi' ]
         ontologies: []
+    - bed:
+        type: file
+        description: "(Optional) The bed regions to merge on"
+        pattern: "*.bed"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -42,27 +48,12 @@ input:
           FILE` parameter)"
         pattern: "*.{fasta,fa}"
         ontologies: []
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
     - fai:
         type: file
         description: "(Optional) The fasta reference file index (only necessary for
           the `--gvcf FILE` parameter)"
         pattern: "*.fai"
         ontologies: []
-  - - meta4:
-        type: map
-        description: |
-          Groovy Map containing bed information
-          e.g. [ id:'genome' ]
-    - bed:
-        type: file
-        description: "(Optional) The bed regions to merge on"
-        pattern: "*.bed"
-        ontologies: []
 output:
   vcf:
     - - meta:
@@ -86,13 +77,27 @@ output:
           description: index of merged output
           pattern: "*.{csi,tbi}"
           ontologies: []
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - "bcftools --version | sed '1!d; s/^.*bcftools //'":
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - "bcftools --version | sed '1!d; s/^.*bcftools //'":
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/bcftools/mpileup/environment.yml b/modules/nf-core/bcftools/mpileup/environment.yml
index 557488607c..ee5cb56dad 100644
--- a/modules/nf-core/bcftools/mpileup/environment.yml
+++ b/modules/nf-core/bcftools/mpileup/environment.yml
@@ -4,4 +4,6 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::bcftools=1.23.1
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/mpileup/main.nf b/modules/nf-core/bcftools/mpileup/main.nf
index f712b5183e..f541694f47 100644
--- a/modules/nf-core/bcftools/mpileup/main.nf
+++ b/modules/nf-core/bcftools/mpileup/main.nf
@@ -1,23 +1,23 @@
 process BCFTOOLS_MPILEUP {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
-    tuple val(meta), path(bam), path(intervals)
-    tuple val(meta2), path(fasta)
+    tuple val(meta), path(bam), path(intervals_mpileup, stageAs: 'mpileup_intervals/*'), path(intervals_call, stageAs: 'call_intervals/*')
+    tuple val(meta2), path(fasta), path(fai)
     val save_mpileup
 
     output:
-    tuple val(meta), path("*vcf.gz")     , emit: vcf
-    tuple val(meta), path("*vcf.gz.tbi") , emit: tbi
-    tuple val(meta), path("*stats.txt")  , emit: stats
+    tuple val(meta), path("*vcf.gz"), emit: vcf
+    tuple val(meta), path("*.{tbi,csi}"), emit: index, optional: true
+    tuple val(meta), path("*stats.txt"), emit: stats
     tuple val(meta), path("*.mpileup.gz"), emit: mpileup, optional: true
-    path  "versions.yml"                 , emit: versions
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -29,31 +29,27 @@ process BCFTOOLS_MPILEUP {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def mpileup = save_mpileup ? "| tee ${prefix}.mpileup" : ""
     def bgzip_mpileup = save_mpileup ? "bgzip ${prefix}.mpileup" : ""
-    def intervals_cmd = intervals ? "-T ${intervals}" : ""
+    def intervals_mpileup_cmd = intervals_mpileup ? "-T ${intervals_mpileup}" : ""
+    def intervals_call_cmd = intervals_call ? "-T ${intervals_call}" : ""
     """
     echo "${meta.id}" > sample_name.list
 
     bcftools \\
         mpileup \\
-        --fasta-ref $fasta \\
-        $args \\
-        $bam \\
-        $intervals_cmd \\
-        $mpileup \\
-        | bcftools call --output-type v $args2 \\
+        --fasta-ref ${fasta} \\
+        ${args} \\
+        ${bam} \\
+        ${intervals_mpileup_cmd} \\
+        ${mpileup} \\
+        | bcftools call --output-type v ${args2} ${intervals_call_cmd} \\
         | bcftools reheader --samples sample_name.list \\
-        | bcftools view --output-file ${prefix}.vcf.gz --output-type z $args3
+        | bcftools view --output-file ${prefix}.vcf.gz --output-type z ${args3}
 
-    $bgzip_mpileup
+    ${bgzip_mpileup}
 
     tabix -p vcf -f ${prefix}.vcf.gz
 
     bcftools stats ${prefix}.vcf.gz > ${prefix}.bcftools_stats.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -63,10 +59,5 @@ process BCFTOOLS_MPILEUP {
     echo "" | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
     echo "" | gzip > ${prefix}.mpileup.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/mpileup/meta.yml b/modules/nf-core/bcftools/mpileup/meta.yml
index febcb33f60..a8134dc7ef 100644
--- a/modules/nf-core/bcftools/mpileup/meta.yml
+++ b/modules/nf-core/bcftools/mpileup/meta.yml
@@ -11,7 +11,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -23,10 +24,17 @@ input:
         type: file
         description: Input BAM file
         pattern: "*.{bam}"
-    - intervals:
+        ontologies: []
+    - intervals_mpileup:
         type: file
-        description: Input intervals file. A file (commonly '.bed') containing regions
-          to subset
+        description: Input intervals file. A file (commonly '.bed') containing
+          regions to subset used by mpileup
+        ontologies: []
+    - intervals_call:
+        type: file
+        description: Input intervals file. A file (commonly '.bed') containing
+          regions to subset used by call but need a fourth column with REF,ALT
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -36,12 +44,18 @@ input:
         type: file
         description: FASTA reference file
         pattern: "*.{fasta,fa}"
-  - - save_mpileup:
-        type: boolean
-        description: Save mpileup file generated by bcftools mpileup
+        ontologies: []
+    - fai:
+        type: file
+        description: FASTA reference file index
+        pattern: "*.fai"
+        ontologies: []
+  - save_mpileup:
+      type: boolean
+      description: Save mpileup file generated by bcftools mpileup
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -50,18 +64,19 @@ output:
           type: file
           description: VCF gzipped output file
           pattern: "*.{vcf.gz}"
-  - tbi:
-      - meta:
+          ontologies: []
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*vcf.gz.tbi":
+      - "*.{tbi,csi}":
           type: file
-          description: tabix index file
-          pattern: "*.{vcf.gz.tbi}"
-  - stats:
-      - meta:
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
+  stats:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -70,8 +85,9 @@ output:
           type: file
           description: Text output file containing stats
           pattern: "*{stats.txt}"
-  - mpileup:
-      - meta:
+          ontologies: []
+  mpileup:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -80,14 +96,29 @@ output:
           type: file
           description: mpileup gzipped output for all positions
           pattern: "{*.mpileup.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@joseespinosa"
-  - "@drpatelh"
 maintainers:
   - "@joseespinosa"
-  - "@drpatelh"
diff --git a/modules/nf-core/bcftools/norm/environment.yml b/modules/nf-core/bcftools/norm/environment.yml
index 557488607c..13b63a623e 100644
--- a/modules/nf-core/bcftools/norm/environment.yml
+++ b/modules/nf-core/bcftools/norm/environment.yml
@@ -4,4 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
+  # renovate: datasource=conda depName=bioconda/bcftools
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf
index 3ad9b35cc2..7de623ed3f 100644
--- a/modules/nf-core/bcftools/norm/main.nf
+++ b/modules/nf-core/bcftools/norm/main.nf
@@ -1,11 +1,11 @@
 process BCFTOOLS_NORM {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
     tuple val(meta), path(vcf), path(tbi)
@@ -13,9 +13,8 @@ process BCFTOOLS_NORM {
 
     output:
     tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
-    tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
-    tuple val(meta), path("*.csi")                    , emit: csi, optional: true
-    path "versions.yml"                               , emit: versions
+    tuple val(meta), path("*.{tbi,csi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,52 +22,49 @@ process BCFTOOLS_NORM {
     script:
     def args = task.ext.args ?: '--output-type z'
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf.gz"
-
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf.gz"
     """
     bcftools norm \\
         --fasta-ref ${fasta} \\
         --output ${prefix}.${extension} \\
-        $args \\
-        --threads $task.cpus \\
+        ${args} \\
+        --threads ${task.cpus} \\
         ${vcf}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
     def args = task.ext.args ?: '--output-type z'
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf.gz"
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf.gz"
     def index = ''
     if (extension in ['vcf.gz', 'bcf', 'bcf.gz']) {
-        if (['--write-index=tbi', '-W=tbi'].any { args.contains(it) }  && extension == 'vcf.gz') {
+        if (['--write-index=tbi', '-W=tbi'].any { arg -> args.contains(arg) } && extension == 'vcf.gz') {
             index = 'tbi'
-        } else if (['--write-index=tbi', '-W=tbi', '--write-index=csi', '-W=csi', '--write-index', '-W'].any { args.contains(it) }) {
+        }
+        else if (['--write-index=tbi', '-W=tbi', '--write-index=csi', '-W=csi', '--write-index', '-W'].any { arg -> args.contains(arg) }) {
             index = 'csi'
         }
     }
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = index ? "touch ${prefix}.${extension}.${index}" : ""
-
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/norm/meta.yml b/modules/nf-core/bcftools/norm/meta.yml
index b6edeb4aae..f465e01eb6 100644
--- a/modules/nf-core/bcftools/norm/meta.yml
+++ b/modules/nf-core/bcftools/norm/meta.yml
@@ -12,7 +12,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -26,11 +27,13 @@ input:
           The vcf file to be normalized
           e.g. 'file1.vcf'
         pattern: "*.{vcf,vcf.gz}"
+        ontologies: []
     - tbi:
         type: file
         description: |
           An optional index of the VCF file (for when the VCF is compressed)
         pattern: "*.vcf.gz.tbi"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -40,43 +43,52 @@ input:
         type: file
         description: FASTA reference file
         pattern: "*.{fasta,fa}"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.{vcf,vcf.gz,bcf,bcf.gz}":
           type: file
-          description: One of uncompressed VCF (.vcf), compressed VCF (.vcf.gz), compressed
-            BCF (.bcf.gz) or uncompressed BCF (.bcf) normalized output file
+          description: One of uncompressed VCF (.vcf), compressed VCF (.vcf.gz),
+            compressed BCF (.bcf.gz) or uncompressed BCF (.bcf) normalized output
+            file
           pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}"
-  - tbi:
-      - meta:
+          ontologies: []
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
+      - "*.{tbi,csi}":
           type: file
-          description: Alternative VCF file index
-          pattern: "*.tbi"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: Default VCF file index
-          pattern: "*.csi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@abhi18av"
   - "@ramprasadn"
diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml
index 557488607c..13b63a623e 100644
--- a/modules/nf-core/bcftools/sort/environment.yml
+++ b/modules/nf-core/bcftools/sort/environment.yml
@@ -4,4 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
+  # renovate: datasource=conda depName=bioconda/bcftools
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf
index 6cbd09b593..2de803014e 100644
--- a/modules/nf-core/bcftools/sort/main.nf
+++ b/modules/nf-core/bcftools/sort/main.nf
@@ -1,20 +1,19 @@
 process BCFTOOLS_SORT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
     tuple val(meta), path(vcf)
 
     output:
     tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
-    tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
-    tuple val(meta), path("*.csi")                    , emit: csi, optional: true
-    path "versions.yml"                               , emit: versions
+    tuple val(meta), path("*.{tbi,csi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -22,49 +21,51 @@ process BCFTOOLS_SORT {
     script:
     def args = task.ext.args ?: '--output-type z'
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def max_memory = task.memory ? "--max-mem ${task.memory.toUnit('MB') * 0.9}M" : ""
     """
     bcftools \\
         sort \\
         --output ${prefix}.${extension} \\
         --temp-dir . \\
-        $args \\
-        $vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
+        ${max_memory} \\
+        ${args} \\
+        ${vcf}
     """
 
     stub:
     def args = task.ext.args ?: '--output-type z'
     def prefix = task.ext.prefix ?: "${meta.id}"
 
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                args.contains("--write-index") || args.contains("-W") ? "csi" :
-                ""
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def index = args.contains("--write-index=tbi") || args.contains("-W=tbi")
+        ? "tbi"
+        : args.contains("--write-index=csi") || args.contains("-W=csi")
+            ? "csi"
+            : args.contains("--write-index") || args.contains("-W")
+                ? "csi"
+                : ""
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : ""
 
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml
index f7a6eff17d..ee0bdb556b 100644
--- a/modules/nf-core/bcftools/sort/meta.yml
+++ b/modules/nf-core/bcftools/sort/meta.yml
@@ -11,7 +11,8 @@ tools:
       documentation: http://www.htslib.org/doc/bcftools.html
       tool_dev_url: https://github.com/samtools/bcftools
       doi: "10.1093/bioinformatics/btp352"
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -23,9 +24,10 @@ input:
         type: file
         description: The VCF/BCF file to be sorted
         pattern: "*.{vcf.gz,vcf,bcf}"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -34,31 +36,38 @@ output:
           type: file
           description: Sorted VCF file
           pattern: "*.{vcf.gz}"
-  - tbi:
-      - meta:
+          ontologies: []
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
+      - "*.{tbi,csi}":
           type: file
-          description: Alternative VCF file index
-          pattern: "*.tbi"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: Default VCF file index
-          pattern: "*.csi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@Gwennid"
 maintainers:
diff --git a/modules/nf-core/bcftools/stats/environment.yml b/modules/nf-core/bcftools/stats/environment.yml
index 7aa06d0f7a..13b63a623e 100644
--- a/modules/nf-core/bcftools/stats/environment.yml
+++ b/modules/nf-core/bcftools/stats/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::bcftools=1.21
-  - bioconda::htslib=1.21
+  # renovate: datasource=conda depName=bioconda/bcftools
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/stats/main.nf b/modules/nf-core/bcftools/stats/main.nf
index fb556e0aa5..6088f15c92 100644
--- a/modules/nf-core/bcftools/stats/main.nf
+++ b/modules/nf-core/bcftools/stats/main.nf
@@ -1,14 +1,14 @@
 process BCFTOOLS_STATS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5acacb55c52bec97c61fd34ffa8721fce82ce823005793592e2a80bf71632cd0/data':
-        'community.wave.seqera.io/library/bcftools:1.21--4335bec1d7b44d11' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
-    tuple val(meta),  path(vcf), path(tbi)
+    tuple val(meta), path(vcf), path(tbi)
     tuple val(meta2), path(regions)
     tuple val(meta3), path(targets)
     tuple val(meta4), path(samples)
@@ -17,7 +17,7 @@ process BCFTOOLS_STATS {
 
     output:
     tuple val(meta), path("*stats.txt"), emit: stats
-    path  "versions.yml"               , emit: versions
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,23 +27,18 @@ process BCFTOOLS_STATS {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def regions_file = regions ? "--regions-file ${regions}" : ""
     def targets_file = targets ? "--targets-file ${targets}" : ""
-    def samples_file =  samples ? "--samples-file ${samples}" : ""
+    def samples_file = samples ? "--samples-file ${samples}" : ""
     def reference_fasta = fasta ? "--fasta-ref ${fasta}" : ""
-    def exons_file = exons      ? "--exons ${exons}" : ""
+    def exons_file = exons ? "--exons ${exons}" : ""
     """
     bcftools stats \\
-        $args \\
-        $regions_file \\
-        $targets_file \\
-        $samples_file \\
-        $reference_fasta \\
-        $exons_file \\
-        $vcf > ${prefix}.bcftools_stats.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
+        ${args} \\
+        ${regions_file} \\
+        ${targets_file} \\
+        ${samples_file} \\
+        ${reference_fasta} \\
+        ${exons_file} \\
+        ${vcf} > ${prefix}.bcftools_stats.txt
     """
 
     stub:
@@ -51,10 +46,5 @@ process BCFTOOLS_STATS {
 
     """
     touch ${prefix}.bcftools_stats.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/stats/meta.yml b/modules/nf-core/bcftools/stats/meta.yml
index 655a61c5f4..1bed6e5656 100644
--- a/modules/nf-core/bcftools/stats/meta.yml
+++ b/modules/nf-core/bcftools/stats/meta.yml
@@ -12,7 +12,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -24,11 +25,13 @@ input:
         type: file
         description: VCF input file
         pattern: "*.{vcf}"
+        ontologies: []
     - tbi:
         type: file
         description: |
           The tab index for the VCF file to be inspected. Optional: only required when parameter regions is chosen.
         pattern: "*.tbi"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -38,6 +41,7 @@ input:
         type: file
         description: |
           Optionally, restrict the operation to regions listed in this file. (VCF, BED or tab-delimited)
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -47,6 +51,7 @@ input:
         type: file
         description: |
           Optionally, restrict the operation to regions listed in this file (doesn't rely upon tbi index files)
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -57,6 +62,7 @@ input:
         description: |
           Optional, file of sample names to be included or excluded.
           e.g. 'file.tsv'
+        ontologies: []
   - - meta5:
         type: map
         description: |
@@ -67,6 +73,7 @@ input:
         description: |
           Tab-delimited file with exons for indel frameshifts (chr,beg,end; 1-based, inclusive, optionally bgzip compressed).
           e.g. 'exons.tsv.gz'
+        ontologies: []
   - - meta6:
         type: map
         description: |
@@ -77,9 +84,10 @@ input:
         description: |
           Faidx indexed reference sequence file to determine INDEL context.
           e.g. 'reference.fa'
+        ontologies: []
 output:
-  - stats:
-      - meta:
+  stats:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -88,11 +96,28 @@ output:
           type: file
           description: Text output file containing stats
           pattern: "*_{stats.txt}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - "bcftools --version | sed '1!d; s/^.*bcftools //'":
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - "bcftools --version | sed '1!d; s/^.*bcftools //'":
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/bcftools/view/environment.yml b/modules/nf-core/bcftools/view/environment.yml
index ba863b388f..13b63a623e 100644
--- a/modules/nf-core/bcftools/view/environment.yml
+++ b/modules/nf-core/bcftools/view/environment.yml
@@ -4,7 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/bcftools
-  - bioconda::bcftools=1.22
+  - bioconda::bcftools=1.23.1
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf
index 72b31200b5..46e8ef5d79 100644
--- a/modules/nf-core/bcftools/view/main.nf
+++ b/modules/nf-core/bcftools/view/main.nf
@@ -1,23 +1,22 @@
 process BCFTOOLS_VIEW {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/47/474a5ea8dc03366b04df884d89aeacc4f8e6d1ad92266888e7a8e7958d07cde8/data':
-        'community.wave.seqera.io/library/bcftools_htslib:0a3fa2654b52006f' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/0b/0b4d52ca9a56d07be3f78a12af654e5116f5112908dba277e6796fd9dfb83fe5/data'
+        : 'community.wave.seqera.io/library/bcftools_htslib:1.23.1--9f08ec665533d64a'}"
 
     input:
     tuple val(meta), path(vcf), path(index)
-    path(regions)
-    path(targets)
-    path(samples)
+    path regions
+    path targets
+    path samples
 
     output:
     tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
-    tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
-    tuple val(meta), path("*.csi")                    , emit: csi, optional: true
-    path "versions.yml"                               , emit: versions
+    tuple val(meta), path("*.{tbi,csi}"), emit: index, optional: true
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), topic: versions, emit: versions_bcftools
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,51 +24,52 @@ process BCFTOOLS_VIEW {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def regions_file  = regions ? "--regions-file ${regions}" : ""
+    def regions_file = regions ? "--regions-file ${regions}" : ""
     def targets_file = targets ? "--targets-file ${targets}" : ""
-    def samples_file =  samples ? "--samples-file ${samples}" : ""
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
+    def samples_file = samples ? "--samples-file ${samples}" : ""
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
     """
     bcftools view \\
         --output ${prefix}.${extension} \\
         ${regions_file} \\
         ${targets_file} \\
         ${samples_file} \\
-        $args \\
-        --threads $task.cpus \\
+        ${args} \\
+        --threads ${task.cpus} \\
         ${vcf}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
-                    args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
-                    args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" :
-                    args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
-                    "vcf"
-    def stub_index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" :
-                     args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" :
-                     args.contains("--write-index")     || args.contains("-W") ? "csi" :
-                     ""
+    def extension = args.contains("--output-type b") || args.contains("-Ob")
+        ? "bcf.gz"
+        : args.contains("--output-type u") || args.contains("-Ou")
+            ? "bcf"
+            : args.contains("--output-type z") || args.contains("-Oz")
+                ? "vcf.gz"
+                : args.contains("--output-type v") || args.contains("-Ov")
+                    ? "vcf"
+                    : "vcf"
+    def stub_index = args.contains("--write-index=tbi") || args.contains("-W=tbi")
+        ? "tbi"
+        : args.contains("--write-index=csi") || args.contains("-W=csi")
+            ? "csi"
+            : args.contains("--write-index") || args.contains("-W")
+                ? "csi"
+                : ""
     def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch"
     def create_index = extension.endsWith(".gz") && stub_index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${stub_index}" : ""
     """
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml
index e9df974206..96351894b0 100644
--- a/modules/nf-core/bcftools/view/meta.yml
+++ b/modules/nf-core/bcftools/view/meta.yml
@@ -1,6 +1,6 @@
 name: bcftools_view
-description: View, subset and filter VCF or BCF files by position and filtering expression.
-  Convert between VCF and BCF
+description: View, subset and filter VCF or BCF files by position and filtering
+  expression. Convert between VCF and BCF
 keywords:
   - variant calling
   - view
@@ -13,7 +13,8 @@ tools:
       homepage: http://samtools.github.io/bcftools/bcftools.html
       documentation: http://www.htslib.org/doc/bcftools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:bcftools
 input:
   - - meta:
@@ -63,35 +64,37 @@ output:
           description: VCF normalized output file
           pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}"
           ontologies: []
-  tbi:
+  index:
     - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
+      - "*.{tbi,csi}":
           type: file
-          description: Alternative VCF file index
-          pattern: "*.tbi"
-          ontologies: []
-  csi:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: Default VCF file index
-          pattern: "*.csi"
-          ontologies: []
+          description: VCF file index
+          pattern: "*.{tbi,csi}"
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@abhi18av"
 maintainers:
diff --git a/modules/nf-core/ngscheckmate/ncm/environment.yml b/modules/nf-core/ngscheckmate/ncm/environment.yml
index 7348216563..20851c4888 100644
--- a/modules/nf-core/ngscheckmate/ncm/environment.yml
+++ b/modules/nf-core/ngscheckmate/ncm/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::ngscheckmate=1.0.1
   - bioconda::bcftools=1.21
+  - bioconda::ngscheckmate=1.0.1
diff --git a/modules/nf-core/ngscheckmate/ncm/main.nf b/modules/nf-core/ngscheckmate/ncm/main.nf
index ffb64a86b5..69b7b14656 100644
--- a/modules/nf-core/ngscheckmate/ncm/main.nf
+++ b/modules/nf-core/ngscheckmate/ncm/main.nf
@@ -2,64 +2,52 @@ process NGSCHECKMATE_NCM {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/ngscheckmate:1.0.1--py312pl5321h577a1d6_4':
-        'biocontainers/ngscheckmate:1.0.1--py312pl5321h577a1d6_4' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/ngscheckmate:1.0.1--py312pl5321h577a1d6_4'
+        : 'quay.io/biocontainers/ngscheckmate:1.0.1--py312pl5321h577a1d6_4'}"
 
     input:
-    tuple val(meta) , path(files)
+    tuple val(meta), path(files)
     tuple val(meta2), path(snp_bed)
-    tuple val(meta3), path(fasta)
+    tuple val(meta3), path(fasta), path(fai)
 
     output:
     tuple val(meta), path("*_corr_matrix.txt"), emit: corr_matrix
-    tuple val(meta), path("*_matched.txt")    , emit: matched
-    tuple val(meta), path("*_all.txt")        , emit: all
-    tuple val(meta), path("*.pdf")            , emit: pdf, optional: true
-    tuple val(meta), path("*.vcf")            , emit: vcf, optional: true
-    path "versions.yml"     , emit: versions
+    tuple val(meta), path("*_matched.txt"),     emit: matched
+    tuple val(meta), path("*_all.txt"),         emit: all
+    tuple val(meta), path("*.pdf"),             emit: pdf, optional: true
+    tuple val(meta), path("*.vcf"),             emit: vcf, optional: true
+    tuple val("${task.process}"), val('ngscheckmate'), eval("ncm.py --help | sed '7!d;s/.* v//g'"), topic: versions, emit: versions_ngscheckmate
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "$meta.id"
-    def unzip = files.any { it.toString().endsWith(".vcf.gz") }
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def unzip = files.any {file ->  file.toString().endsWith(".vcf.gz") }
     """
-    if $unzip
+    if ${unzip}
     then
         for VCFGZ in *.vcf.gz; do
             gunzip -cdf \$VCFGZ > \$( basename \$VCFGZ .gz );
         done
     fi
 
-    NCM_REF="./"${fasta} ncm.py -d . -bed ${snp_bed} -O . -N ${prefix} $args
+    NCM_REF="./"${fasta} ncm.py -d . -bed ${snp_bed} -O . -N ${prefix} ${args}
 
-    if $unzip
+    if ${unzip}
     then
         rm -f *.vcf  # clean up decompressed vcfs
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ngscheckmate: \$(ncm.py --help | sed "7!d;s/ *Ensuring Sample Identity v//g")
-    END_VERSIONS
     """
 
     stub:
-    def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "$meta.id"
+    def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}_output_corr_matrix.txt
     touch ${prefix}_matched.txt
     touch ${prefix}_all.txt
     touch ${prefix}.pdf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ngscheckmate: \$(ncm.py --help | sed "7!d;s/ *Ensuring Sample Identity v//g")
-    END_VERSIONS
     """
-
 }
diff --git a/modules/nf-core/ngscheckmate/ncm/meta.yml b/modules/nf-core/ngscheckmate/ncm/meta.yml
index 06c131d6b0..ea031b4aa9 100644
--- a/modules/nf-core/ngscheckmate/ncm/meta.yml
+++ b/modules/nf-core/ngscheckmate/ncm/meta.yml
@@ -27,6 +27,7 @@ input:
         description: VCF or BAM files for each sample, in a merged channel (possibly
           gzipped). BAM files require an index too.
         pattern: "*.{vcf,vcf.gz,bam,bai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -36,6 +37,7 @@ input:
         type: file
         description: BED file containing the SNPs to analyse
         pattern: "*.{bed}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -44,62 +46,93 @@ input:
     - fasta:
         type: file
         description: fasta file for the genome, only used in the bam mode
-        pattern: "*.{bed}"
+        pattern: "*.{fasta}"
+        ontologies: []
+    - fai:
+        type: file
+        description: fasta index file for the genome, only used in the bam mode
+        pattern: "*.{fai}"
+        ontologies: []
 output:
-  - corr_matrix:
-      - meta:
+  corr_matrix:
+    - - meta:
           type: file
           description: A text file containing the correlation matrix between each sample
           pattern: "*corr_matrix.txt"
+          ontologies: []
       - "*_corr_matrix.txt":
           type: file
           description: A text file containing the correlation matrix between each sample
           pattern: "*corr_matrix.txt"
-  - matched:
-      - meta:
+          ontologies: []
+  matched:
+    - - meta:
           type: file
-          description: A txt file containing only the samples that match with each other
-          pattern: "*matched.txt"
+          description: A text file containing the correlation matrix between each sample
+          pattern: "*corr_matrix.txt"
+          ontologies: []
       - "*_matched.txt":
           type: file
           description: A txt file containing only the samples that match with each other
           pattern: "*matched.txt"
-  - all:
-      - meta:
+          ontologies: []
+  all:
+    - - meta:
           type: file
-          description: A txt file containing all the sample comparisons, whether they
-            match or not
-          pattern: "*all.txt"
+          description: A text file containing the correlation matrix between each sample
+          pattern: "*corr_matrix.txt"
+          ontologies: []
       - "*_all.txt":
           type: file
           description: A txt file containing all the sample comparisons, whether they
             match or not
           pattern: "*all.txt"
-  - pdf:
-      - meta:
+          ontologies: []
+  pdf:
+    - - meta:
           type: file
-          description: A pdf containing a dendrogram showing how the samples match up
-          pattern: "*.{pdf}"
+          description: A text file containing the correlation matrix between each sample
+          pattern: "*corr_matrix.txt"
+          ontologies: []
       - "*.pdf":
           type: file
           description: A pdf containing a dendrogram showing how the samples match up
           pattern: "*.{pdf}"
-  - vcf:
-      - meta:
+          ontologies: []
+  vcf:
+    - - meta:
           type: file
-          description: If ran in bam mode, vcf files for each sample giving the SNP calls
-            used
-          pattern: "*.vcf"
+          description: A text file containing the correlation matrix between each sample
+          pattern: "*corr_matrix.txt"
+          ontologies: []
       - "*.vcf":
           type: file
-          description: If ran in bam mode, vcf files for each sample giving the SNP calls
-            used
+          description: If ran in bam mode, vcf files for each sample giving the SNP
+            calls used
           pattern: "*.vcf"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_ngscheckmate:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - ngscheckmate:
+          type: string
+          description: The tool name
+      - "ncm.py --help | sed '7!d;s/.* v//g'":
+          type: string
+          description: The command used to generate the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - ngscheckmate:
+          type: string
+          description: The tool name
+      - "ncm.py --help | sed '7!d;s/.* v//g'":
+          type: string
+          description: The command used to generate the version of the tool
 authors:
   - "@sppearce"
 maintainers:
diff --git a/subworkflows/local/bam_variant_calling_freebayes/main.nf b/subworkflows/local/bam_variant_calling_freebayes/main.nf
index 18ef51f5e5..4708d06750 100644
--- a/subworkflows/local/bam_variant_calling_freebayes/main.nf
+++ b/subworkflows/local/bam_variant_calling_freebayes/main.nf
@@ -64,7 +64,6 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     // Index the filtered VCFs
     TABIX_VC_FREEBAYES_FILT(vcf_filtered)
 
-    versions = versions.mix(BCFTOOLS_SORT.out.versions)
     versions = versions.mix(FREEBAYES.out.versions)
     versions = versions.mix(TABIX_VC_FREEBAYES.out.versions)
     versions = versions.mix(TABIX_VC_FREEBAYES_FILT.out.versions)
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index cc28669740..5da093ab4b 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -84,6 +84,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             cram,
             dict,
             fasta,
+            fasta_fai,
             intervals
         )
         vcf_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.vcf
diff --git a/subworkflows/local/bam_variant_calling_mpileup/main.nf b/subworkflows/local/bam_variant_calling_mpileup/main.nf
index 7b7765f2c3..5e21480c21 100644
--- a/subworkflows/local/bam_variant_calling_mpileup/main.nf
+++ b/subworkflows/local/bam_variant_calling_mpileup/main.nf
@@ -13,7 +13,8 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     take:
     cram      // channel: [mandatory] [ meta, cram, crai ]
     dict      // channel: [mandatory] [ meta, dict ]
-    fasta     // channel: [mandatory] [ fasta ]
+    fasta     // channel: [mandatory] [ meta, fasta ]
+    fai       // channel: [mandatory] [ meta, fasta_fai ]
     intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
@@ -24,9 +25,14 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
         .combine(intervals)
         .map { meta, cram_, _crai, intervals_, num_intervals -> [meta + [num_intervals: num_intervals], cram_, intervals_] }
 
+    // per-chunk bed restricts mpileup only; intervals_call left empty
+    cram_intervals_bcftools = cram_intervals.map { meta, cram_, intervals_ -> [meta, cram_, intervals_, []] }
+
+    fasta_fai = fasta.combine(fai).map { meta, fasta_, _meta2, fai_ -> [meta, fasta_, fai_] }.collect()
+
     // Run, if --tools mpileup
     keep_bcftools_mpileup = false
-    BCFTOOLS_MPILEUP(cram_intervals, fasta, keep_bcftools_mpileup)
+    BCFTOOLS_MPILEUP(cram_intervals_bcftools, fasta_fai, keep_bcftools_mpileup)
 
     //Only run, if --tools ControlFreec
     SAMTOOLS_MPILEUP(cram_intervals, fasta)
@@ -38,7 +44,7 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_mpileup = BCFTOOLS_MPILEUP.out.tbi.branch {
+    tbi_mpileup = BCFTOOLS_MPILEUP.out.index.branch {
         intervals: it[0].num_intervals > 1
         no_intervals: it[0].num_intervals <= 1
     }
@@ -69,7 +75,6 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
         .map { meta, tbi -> [meta - meta.subMap('num_intervals') + [variantcaller: 'bcftools'], tbi] }
 
     versions = versions.mix(SAMTOOLS_MPILEUP.out.versions)
-    versions = versions.mix(BCFTOOLS_MPILEUP.out.versions)
     versions = versions.mix(CAT_MPILEUP.out.versions)
 
     emit:
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index f22e36d656..f43549b2f1 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -95,6 +95,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             cram_normal,
             dict,
             fasta,
+            fasta_fai,
             intervals,
         )
 
@@ -102,6 +103,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             cram_tumor,
             dict,
             fasta,
+            fasta_fai,
             intervals,
         )
 
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 240cb3a73a..0613a74b8b 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -72,6 +72,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
             cram,
             dict,
             fasta,
+            fasta_fai,
             intervals,
         )
         vcf_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.vcf
diff --git a/subworkflows/local/cram_sampleqc/main.nf b/subworkflows/local/cram_sampleqc/main.nf
index 1e664186f7..c3be937964 100644
--- a/subworkflows/local/cram_sampleqc/main.nf
+++ b/subworkflows/local/cram_sampleqc/main.nf
@@ -5,20 +5,19 @@ workflow CRAM_SAMPLEQC {
     take:
     cram                        // channel: [ val(meta), cram, crai ]
     ngscheckmate_bed            // channel: [ ngscheckmate_bed ]
-    fasta                       // channel: [ fasta ]
+    fasta_fai                   // channel: [ val(meta), fasta, fasta_fai ]
     skip_baserecalibration      // boolean:
     intervals_for_preprocessing // channel:
 
     main:
-
-    versions = Channel.empty()
-    reports = Channel.empty()
+    versions = channel.empty()
+    reports = channel.empty()
 
     if (!skip_baserecalibration) {
 
         CRAM_QC_RECAL(
             cram,
-            fasta,
+            fasta_fai.map{meta, fasta, _fai -> [meta, fasta]},
             intervals_for_preprocessing,
         )
 
@@ -29,8 +28,7 @@ workflow CRAM_SAMPLEQC {
         versions = versions.mix(CRAM_QC_RECAL.out.versions)
     }
 
-    BAM_NGSCHECKMATE(cram.map { meta, cram_, _crai -> [meta, cram_] }, ngscheckmate_bed.map { bed -> [[id: "ngscheckmate"], bed] }, fasta)
-    versions = versions.mix(BAM_NGSCHECKMATE.out.versions)
+    BAM_NGSCHECKMATE(cram.map { meta, cram_, _crai -> [meta, cram_] }, ngscheckmate_bed.map { bed -> [[id: "ngscheckmate"], bed] }, fasta_fai)
 
     emit:
     corr_matrix = BAM_NGSCHECKMATE.out.corr_matrix // channel: [ meta, corr_matrix ]
diff --git a/subworkflows/local/post_variantcalling/main.nf b/subworkflows/local/post_variantcalling/main.nf
index 4afd0b576e..277bed9765 100644
--- a/subworkflows/local/post_variantcalling/main.nf
+++ b/subworkflows/local/post_variantcalling/main.nf
@@ -50,7 +50,6 @@ workflow POST_VARIANTCALLING {
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.tbi)
-        versions = versions.mix(VCF_VARLOCIRAPTOR_GERMLINE.out.versions)
 
         // SOMATIC
         VCF_VARLOCIRAPTOR_SOMATIC(cram_somatic, fasta, fai, varlociraptor_scenario_somatic, somatic_vcfs, germline_vcfs, varlociraptor_chunk_size, varlociraptor_events_somatic, varlociraptor_fdr)
@@ -64,7 +63,6 @@ workflow POST_VARIANTCALLING {
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_TUMOR_ONLY.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_TUMOR_ONLY.out.tbi)
-        versions = versions.mix(VCF_VARLOCIRAPTOR_TUMOR_ONLY.out.versions)
 
     } else if (filter_vcfs || normalize_vcfs || concatenate_vcfs ) {
 
@@ -118,8 +116,7 @@ workflow POST_VARIANTCALLING {
             FILTER_VCFS( small_variant_vcfs.join(small_variant_tbis, failOnDuplicate: true, failOnMismatch: true), [], [], [])
 
             small_variant_vcfs = FILTER_VCFS.out.vcf
-            small_variant_tbis = FILTER_VCFS.out.tbi
-            versions = versions.mix(FILTER_VCFS.out.versions)
+            small_variant_tbis = FILTER_VCFS.out.index
         }
 
         if (normalize_vcfs) {
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 3b8de518e7..5460704157 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -153,7 +153,7 @@ workflow PREPARE_GENOME {
     if (!bcftools_annotations_tbi_in && bcftools_annotations_in) {
         TABIX_BCFTOOLS_ANNOTATIONS(bcftools_annotations.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
         bcftools_annotations_tbi = TABIX_BCFTOOLS_ANNOTATIONS.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_BCFTOOLS_ANNOTATIONS.out.versions)
+
     }
 
     dbsnp = dbsnp_in ? Channel.fromPath(dbsnp_in).collect() : Channel.value([])
diff --git a/subworkflows/local/vcf_annotate_all/main.nf b/subworkflows/local/vcf_annotate_all/main.nf
index 72f9dfe061..ea559ed6c0 100644
--- a/subworkflows/local/vcf_annotate_all/main.nf
+++ b/subworkflows/local/vcf_annotate_all/main.nf
@@ -34,14 +34,10 @@ workflow VCF_ANNOTATE_ALL {
 
     if (tools.split(',').contains('bcfann')) {
         BCFTOOLS_ANNOTATE(
-            vcf.map { meta, vcf_ -> [meta, vcf_, []] }.combine(bcftools_annotations).combine(bcftools_annotations_index),
-            bcftools_columns,
-            bcftools_header_lines,
-            [],
+            vcf.map { meta, vcf_ -> [meta, vcf_, []] }.combine(bcftools_annotations).combine(bcftools_annotations_index).combine(bcftools_columns).combine(bcftools_header_lines).map{meta, vcf_, index, ann, ann_index, cols, header -> [meta, vcf_, index, ann, ann_index, cols, header, []]}
         )
 
-        vcf_ann = vcf_ann.mix(BCFTOOLS_ANNOTATE.out.vcf.join(BCFTOOLS_ANNOTATE.out.tbi, failOnDuplicate: true, failOnMismatch: true))
-        versions = versions.mix(BCFTOOLS_ANNOTATE.out.versions)
+        vcf_ann = vcf_ann.mix(BCFTOOLS_ANNOTATE.out.vcf.join(BCFTOOLS_ANNOTATE.out.index, failOnDuplicate: true, failOnMismatch: true))
     }
 
     if (tools.split(',').contains('merge') || tools.split(',').contains('snpeff')) {
diff --git a/subworkflows/local/vcf_concatenate_germline/main.nf b/subworkflows/local/vcf_concatenate_germline/main.nf
index 31dc6de01b..3c3010666f 100644
--- a/subworkflows/local/vcf_concatenate_germline/main.nf
+++ b/subworkflows/local/vcf_concatenate_germline/main.nf
@@ -28,11 +28,9 @@ workflow CONCATENATE_GERMLINE_VCFS {
 
     // Gather versions of all tools used
     versions = versions.mix(ADD_INFO_TO_VCF.out.versions)
-    versions = versions.mix(GERMLINE_VCFS_CONCAT.out.versions)
-    versions = versions.mix(GERMLINE_VCFS_CONCAT_SORT.out.versions)
 
     emit:
     vcfs     = GERMLINE_VCFS_CONCAT_SORT.out.vcf // concatenated vcfs
-    tbis     = GERMLINE_VCFS_CONCAT_SORT.out.tbi // matching tbis
+    tbis     = GERMLINE_VCFS_CONCAT_SORT.out.index // matching tbis
     versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/vcf_consensus/main.nf b/subworkflows/local/vcf_consensus/main.nf
index aa18c0c939..0b9dbb028f 100644
--- a/subworkflows/local/vcf_consensus/main.nf
+++ b/subworkflows/local/vcf_consensus/main.nf
@@ -32,12 +32,11 @@ workflow CONSENSUS {
         .groupTuple(size: 2)
 
     BCFTOOLS_CONCAT(ch_strelka_grouped)// somatic strelkas have two vcf files: SNPs and indels
-    ch_versions = ch_versions.mix(BCFTOOLS_CONCAT.out.versions)
 
     // Combine concat strelka with remaining VCFs
     // Bundle each VCF with its caller to preserve association through grouping
     ch_consensus_in = ch_vcfs.other
-                        .mix(BCFTOOLS_CONCAT.out.vcf.join(BCFTOOLS_CONCAT.out.tbi))
+                        .mix(BCFTOOLS_CONCAT.out.vcf.join(BCFTOOLS_CONCAT.out.index))
                         .map { meta, vcf, tbi ->
                                     def caller = meta.variantcaller
                                     def groupKey = meta - meta.subMap('variantcaller', 'contamination', 'filename', 'data_type', 'num_intervals')
@@ -53,12 +52,13 @@ workflow CONSENSUS {
                             def sorted_pairs = vcf_caller_pairs.sort { a, b -> a[0].name <=> b[0].name }
                             def sorted_vcfs = sorted_pairs.collect { it[0] }
                             def callers = sorted_pairs.collect { it[1] }
-                            [meta + [callers: callers], sorted_vcfs, tbis]
+                            // file_list, targets_file, regions_file are unused: VCFs are passed positionally
+                            // and consensus is computed genome-wide (no region/target restriction)
+                            [meta + [callers: callers], sorted_vcfs, tbis, [], [], []]
                         }
 
 
     BCFTOOLS_ISEC(ch_consensus_in)
-    ch_versions = ch_versions.mix(BCFTOOLS_ISEC.out.versions)
 
     // Filter out empty isec results (no consensus variants found)
     ch_isec_with_results = BCFTOOLS_ISEC.out.results
diff --git a/subworkflows/local/vcf_normalization/main.nf b/subworkflows/local/vcf_normalization/main.nf
index afe7b350b2..8878eeacd3 100644
--- a/subworkflows/local/vcf_normalization/main.nf
+++ b/subworkflows/local/vcf_normalization/main.nf
@@ -29,11 +29,9 @@ workflow NORMALIZE_VCFS {
 
     // Gather versions of all tools used
     versions = versions.mix(ADD_INFO_TO_VCF.out.versions)
-    versions = versions.mix(VCFS_NORM.out.versions)
-    versions = versions.mix(VCFS_NORM_SORT.out.versions)
 
     emit:
-    vcfs     = VCFS_NORM_SORT.out.vcf // normalized vcfs
-    tbis     = VCFS_NORM_SORT.out.tbi // matching tbis
+    vcfs     = VCFS_NORM_SORT.out.vcf   // normalized vcfs
+    tbis     = VCFS_NORM_SORT.out.index // matching tbis
     versions // Channel: [versions.yml]
 }
diff --git a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
index ab69bdeb15..21e4ce927a 100644
--- a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
+++ b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
@@ -17,7 +17,6 @@ workflow VCF_QC_BCFTOOLS_VCFTOOLS {
     VCFTOOLS_TSTV_QUAL(vcf, target_bed, [])
     VCFTOOLS_SUMMARY(vcf, target_bed, [])
 
-    versions = versions.mix(BCFTOOLS_STATS.out.versions)
     versions = versions.mix(VCFTOOLS_TSTV_COUNT.out.versions)
 
     emit:
diff --git a/subworkflows/local/vcf_varlociraptor_single/main.nf b/subworkflows/local/vcf_varlociraptor_single/main.nf
index 2ba55d4af2..27232307fd 100644
--- a/subworkflows/local/vcf_varlociraptor_single/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_single/main.nf
@@ -21,8 +21,6 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
     val_fdr
 
     main:
-    ch_versions = channel.empty()
-
     meta_map = ch_cram.map { meta, _cram, _crai -> meta + [sex_string: (meta.sex == "XX" ? "female" : "male")] }
 
     FILL_SCENARIO_FILE(
@@ -110,14 +108,13 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
     SORT_CALLED_CHUNKS(
         VARLOCIRAPTOR_CALLVARIANTS.out.bcf
     )
-    ch_versions = ch_versions.mix(SORT_CALLED_CHUNKS.out.versions)
 
     ch_sort_called_chunks_vcf = SORT_CALLED_CHUNKS.out.vcf.branch {
         single: val_num_chunks <= 1
         multiple: val_num_chunks > 1
     }
 
-    ch_sort_called_chunks_tbi = SORT_CALLED_CHUNKS.out.tbi.branch {
+    ch_sort_called_chunks_tbi = SORT_CALLED_CHUNKS.out.index.branch {
         single: val_num_chunks <= 1
         multiple: val_num_chunks > 1
     }
@@ -131,8 +128,6 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
 
     CONCAT_CALLED_CHUNKS(ch_vcf_tbi_chunks)
 
-    ch_versions = ch_versions.mix(CONCAT_CALLED_CHUNKS.out.versions)
-
     ch_final_vcf = ch_sort_called_chunks_vcf.single.mix(CONCAT_CALLED_CHUNKS.out.vcf)
 
     VARLOCIRAPTOR_FILTERFDR(
@@ -141,10 +136,7 @@ workflow VCF_VARLOCIRAPTOR_SINGLE {
 
     SORT_FINAL_VCF(VARLOCIRAPTOR_FILTERFDR.out.bcf)
 
-    ch_versions = ch_versions.mix(SORT_FINAL_VCF.out.versions)
-
     emit:
     vcf      = SORT_FINAL_VCF.out.vcf
-    tbi      = SORT_FINAL_VCF.out.tbi
-    versions = ch_versions
+    tbi      = SORT_FINAL_VCF.out.index
 }
diff --git a/subworkflows/local/vcf_varlociraptor_somatic/main.nf b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
index 4ad4c80cf5..292c18b340 100644
--- a/subworkflows/local/vcf_varlociraptor_somatic/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
@@ -79,7 +79,7 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
 
     // Use concatenated Strelka VCFs for somatic and germline calling, mix with other variant callers
     ch_somatic_vcf_conc = CONCAT_SOMATIC_STRELKA.out.vcf
-        .join(CONCAT_SOMATIC_STRELKA.out.tbi, by: [0])
+        .join(CONCAT_SOMATIC_STRELKA.out.index, by: [0])
         .mix(ch_somatic_branched.other)
 
     //
@@ -107,15 +107,11 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
 
     MERGE_GERMLINE_SOMATIC_VCFS(
         branched.matched.map { _key, meta_somatic, somatic_vcf, somatic_tbi, _meta_germline, germline_vcf, germline_tbi ->
-            [meta_somatic, [somatic_vcf, germline_vcf], [somatic_tbi, germline_tbi]]
+            [meta_somatic, [somatic_vcf, germline_vcf], [somatic_tbi, germline_tbi], []]
         },
-        ch_fasta,
-        ch_fasta_fai,
-        [[], []],
+        ch_fasta.combine(ch_fasta_fai).map{ meta_fasta_, fasta, _meta_fai, fai -> [meta_fasta_, fasta, fai]}.collect()
     )
 
-    ch_versions = ch_versions.mix(MERGE_GERMLINE_SOMATIC_VCFS.out.versions)
-
     // Combine merged VCFs with unmatched somatic VCFs
     ch_vcf = MERGE_GERMLINE_SOMATIC_VCFS.out.vcf.mix(
         branched.unmatched.map { _key, meta, vcf, _tbi -> [meta, vcf] }
@@ -261,14 +257,13 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     SORT_CALLED_CHUNKS(
         VARLOCIRAPTOR_CALLVARIANTS.out.bcf
     )
-    ch_versions = ch_versions.mix(SORT_CALLED_CHUNKS.out.versions)
 
     ch_sort_called_chunks_vcf = SORT_CALLED_CHUNKS.out.vcf.branch {
         single: val_num_chunks <= 1
         multiple: val_num_chunks > 1
     }
 
-    ch_sort_called_chunks_tbi = SORT_CALLED_CHUNKS.out.tbi.branch {
+    ch_sort_called_chunks_tbi = SORT_CALLED_CHUNKS.out.index.branch {
         single: val_num_chunks <= 1
         multiple: val_num_chunks > 1
     }
@@ -282,8 +277,6 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
 
     CONCAT_CALLED_CHUNKS(ch_vcf_tbi_chunks)
 
-    ch_versions = ch_versions.mix(CONCAT_CALLED_CHUNKS.out.versions)
-
     ch_final_vcf = ch_sort_called_chunks_vcf.single.mix(CONCAT_CALLED_CHUNKS.out.vcf)
 
     VARLOCIRAPTOR_FILTERFDR(
@@ -292,10 +285,8 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
 
     SORT_FINAL_VCF(VARLOCIRAPTOR_FILTERFDR.out.bcf)
 
-    ch_versions = ch_versions.mix(SORT_FINAL_VCF.out.versions)
-
     emit:
     vcf      = SORT_FINAL_VCF.out.vcf
-    tbi      = SORT_FINAL_VCF.out.tbi
+    tbi      = SORT_FINAL_VCF.out.index
     versions = ch_versions
 }
diff --git a/subworkflows/nf-core/bam_ngscheckmate/main.nf b/subworkflows/nf-core/bam_ngscheckmate/main.nf
index d698dd3f24..71f3e7b12f 100644
--- a/subworkflows/nf-core/bam_ngscheckmate/main.nf
+++ b/subworkflows/nf-core/bam_ngscheckmate/main.nf
@@ -1,24 +1,21 @@
 include { BCFTOOLS_MPILEUP } from '../../../modules/nf-core/bcftools/mpileup/main'
 include { NGSCHECKMATE_NCM } from '../../../modules/nf-core/ngscheckmate/ncm/main'
-
+// please note this subworkflow requires the options for bcltools_mpileup that are included in the nextflow.config
 workflow BAM_NGSCHECKMATE {
 
     take:
     ch_input            // channel: [ val(meta1), bam/cram ]
     ch_snp_bed          // channel: [ val(meta2), bed ]
-    ch_fasta            // channel: [ val(meta3), fasta ]
+    ch_fasta            // channel: [ val(meta3), fasta, fai ]
 
     main:
-
-    ch_versions = Channel.empty()
-    ch_input_bed = ch_input.combine(ch_snp_bed)
-                        // do something to combine the metas?
-                        .map{ input_meta, input_file, _bed_meta, bed_file ->
-                            [input_meta, input_file, bed_file]
-                        }
+    ch_input_bed = ch_input
+        .combine(ch_snp_bed)
+        .map{ input_meta, input_file, _bed_meta, bed_file ->
+            [input_meta, input_file, bed_file, []]
+        }
 
     BCFTOOLS_MPILEUP (ch_input_bed, ch_fasta.collect(), false)
-    ch_versions = ch_versions.mix(BCFTOOLS_MPILEUP.out.versions)
 
     BCFTOOLS_MPILEUP
     .out
@@ -34,7 +31,6 @@ workflow BAM_NGSCHECKMATE {
     .set {ch_vcfs}
 
     NGSCHECKMATE_NCM (ch_vcfs, ch_snp_bed, ch_fasta)
-    ch_versions = ch_versions.mix(NGSCHECKMATE_NCM.out.versions)
 
     emit:
     corr_matrix  = NGSCHECKMATE_NCM.out.corr_matrix  // channel: [ meta, corr_matrix ]
@@ -42,6 +38,5 @@ workflow BAM_NGSCHECKMATE {
     all          = NGSCHECKMATE_NCM.out.all          // channel: [ meta, all ]
     vcf          = BCFTOOLS_MPILEUP.out.vcf          // channel: [ meta, vcf ]
     pdf          = NGSCHECKMATE_NCM.out.pdf          // channel: [ meta, pdf ]
-    versions     = ch_versions                       // channel: [ versions.yml ]
 
 }
diff --git a/subworkflows/nf-core/bam_ngscheckmate/meta.yml b/subworkflows/nf-core/bam_ngscheckmate/meta.yml
index 7de0a114d4..ce45cbba2c 100644
--- a/subworkflows/nf-core/bam_ngscheckmate/meta.yml
+++ b/subworkflows/nf-core/bam_ngscheckmate/meta.yml
@@ -37,6 +37,10 @@ input:
       type: file
       description: fasta file for the genome
       pattern: "*.{fasta}"
+  - fai:
+      type: file
+      description: fasta file index for the genome
+      pattern: "*.{fai}"
 output:
   - pdf:
       type: file
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index 2b4186d1f0..5f31c21071 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -473,7 +473,7 @@
             26,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -747,7 +747,7 @@
                 "chr22_1-40001.bed.gz:md5,87a15eb9c2ff20ccd5cd8735a28708f7",
                 "genome.bed:md5,a87dc7d20ebca626f65cc16ff6c97a3e",
                 "genome.bed.gz:md5,a87dc7d20ebca626f65cc16ff6c97a3e",
-                "test2_vs_test.mutect2.filtered.bcftools_stats.txt:md5,46384bbd90894ff023684d88df891fd6",
+                "test2_vs_test.mutect2.filtered.bcftools_stats.txt:md5,bd7378d60c4466257489fae86e9de3da",
                 "test.recal.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.recal.mosdepth.region.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.recal.mosdepth.summary.txt:md5,2ea3534987f28b3ed0b64a8e7986b442",
diff --git a/tests/annotation_bcfann.nf.test.snap b/tests/annotation_bcfann.nf.test.snap
index 9d1781890e..4b3cde2636 100644
--- a/tests/annotation_bcfann.nf.test.snap
+++ b/tests/annotation_bcfann.nf.test.snap
@@ -4,7 +4,7 @@
             2,
             {
                 "BCFTOOLS_ANNOTATE": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 }
             },
             [
@@ -48,7 +48,7 @@
             2,
             {
                 "BCFTOOLS_ANNOTATE": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 }
             },
             [
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index 8f7541248e..ea7c990001 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -10,7 +10,7 @@
                     "bbmap": "39.18"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -343,7 +343,7 @@
                 "chr22_1-40001.bed.gz:md5,87a15eb9c2ff20ccd5cd8735a28708f7",
                 "genome.bed:md5,a87dc7d20ebca626f65cc16ff6c97a3e",
                 "genome.bed.gz:md5,a87dc7d20ebca626f65cc16ff6c97a3e",
-                "test.strelka.variants.bcftools_stats.txt:md5,b6511034fcbf5c396ed035207b4aea41",
+                "test.strelka.variants.bcftools_stats.txt:md5,0eaa13927e8db9f3d56afdc6cf60ffad",
                 "test.strelka.variants.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -374,7 +374,7 @@
                     "bbmap": "39.18"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index ffe0be0437..38bfde8a8b 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -4,7 +4,7 @@
             23,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -293,7 +293,7 @@
                 "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
-                "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
+                "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
                 "test.md.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
@@ -331,7 +331,7 @@
             23,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
diff --git a/tests/joint_calling_haplotypecaller.nf.test.snap b/tests/joint_calling_haplotypecaller.nf.test.snap
index 9f724dd5a7..dd7b6e2735 100644
--- a/tests/joint_calling_haplotypecaller.nf.test.snap
+++ b/tests/joint_calling_haplotypecaller.nf.test.snap
@@ -3,8 +3,11 @@
         "content": [
             26,
             {
+                "BCFTOOLS_SORT": {
+                    "bcftools": "1.23.1"
+                },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -190,7 +193,7 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "joint_germline.bcftools_stats.txt:md5,1e0bcb3e7dc0e812371e4609f477569c",
+                "joint_germline.bcftools_stats.txt:md5,8d7de0e9bbf007cf6c80caaa5e6e9ea2",
                 "testN.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "testN.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "testN.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
@@ -249,8 +252,11 @@
         "content": [
             18,
             {
+                "BCFTOOLS_SORT": {
+                    "bcftools": "1.23.1"
+                },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -433,7 +439,7 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "joint_germline.bcftools_stats.txt:md5,1e0bcb3e7dc0e812371e4609f477569c",
+                "joint_germline.bcftools_stats.txt:md5,8d7de0e9bbf007cf6c80caaa5e6e9ea2",
                 "testN.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "testN.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "testN.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 7a79997e91..6d94c5ade1 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -4,7 +4,7 @@
             17,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -188,7 +188,7 @@
                 "samtools-stats-dp.txt:md5,fe7c4ec200c94df7ff97a0e8cbec2f70",
                 "samtools_alignment_plot.txt:md5,82d6fb277fbbbd221a92ebda4567d9d9",
                 "samtools_insert_size.txt:md5,00660bb059693565c1189ce98356e414",
-                "test.mutect2.bcftools_stats.txt:md5,45096ddcb6f27a59224b19a8a7c6b1ac",
+                "test.mutect2.bcftools_stats.txt:md5,2de4f9f8da53b202e9d784b4e95c3245",
                 "sample1.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample1.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample1.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
@@ -235,7 +235,7 @@
             16,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BUILD_INTERVALS": {
                     "gawk": "5.3.0"
@@ -412,7 +412,7 @@
                 "samtools-stats-dp.txt:md5,e6019a625137ced6d426a503cd652559",
                 "samtools_alignment_plot.txt:md5,cb01fb538637d281195e46e557031c7a",
                 "samtools_insert_size.txt:md5,97989575c42dadc81f347d661faa09cb",
-                "test.mutect2.bcftools_stats.txt:md5,9114eb52e42ff6b8475e7a7773c21eee",
+                "test.mutect2.bcftools_stats.txt:md5,3079129e89fdaf04a3403d888a99fd81",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,b0b47739dcafeeb1a9e6218b8abca1e0",
diff --git a/tests/postprocess_concatenation.nf.test.snap b/tests/postprocess_concatenation.nf.test.snap
index 172d2969fa..7c206ba449 100644
--- a/tests/postprocess_concatenation.nf.test.snap
+++ b/tests/postprocess_concatenation.nf.test.snap
@@ -7,10 +7,10 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -22,10 +22,10 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GERMLINE_VCFS_CONCAT_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -244,10 +244,10 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "testN.freebayes.filtered.bcftools_stats.txt:md5,96c5e6b9e55f1bb67fca7886fb322b67",
-                "testT.freebayes.filtered.bcftools_stats.txt:md5,ce8b7afaace836b6a00bd8ccdc980500",
-                "testN.strelka.variants.bcftools_stats.txt:md5,f11c9e5e9820868809b5465970c7cc06",
-                "testT.strelka.variants.bcftools_stats.txt:md5,ac070b6cf4de7540a227265971244b31",
+                "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
+                "testT.freebayes.filtered.bcftools_stats.txt:md5,a5beebaf9e38ab8e3e9c332c46dd9ae1",
+                "testN.strelka.variants.bcftools_stats.txt:md5,fcc53db2f8001b3a68673a9e0802e06c",
+                "testT.strelka.variants.bcftools_stats.txt:md5,d8dd5a46552458e72ea9963e948a377c",
                 "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
diff --git a/tests/postprocess_concatenation_normalization.nf.test.snap b/tests/postprocess_concatenation_normalization.nf.test.snap
index d663d6bc66..d0db0839ff 100644
--- a/tests/postprocess_concatenation_normalization.nf.test.snap
+++ b/tests/postprocess_concatenation_normalization.nf.test.snap
@@ -7,16 +7,16 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FILTER_VCFS": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "FREEBAYES": {
                     "freebayes": "1.3.10"
@@ -25,10 +25,10 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GERMLINE_VCFS_CONCAT_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -61,10 +61,10 @@
                     "vcflib": "1.0.14"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -275,10 +275,10 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "testN.freebayes.filtered.bcftools_stats.txt:md5,96c5e6b9e55f1bb67fca7886fb322b67",
-                "testT.freebayes.filtered.bcftools_stats.txt:md5,ce8b7afaace836b6a00bd8ccdc980500",
-                "testN.strelka.variants.bcftools_stats.txt:md5,f11c9e5e9820868809b5465970c7cc06",
-                "testT.strelka.variants.bcftools_stats.txt:md5,ac070b6cf4de7540a227265971244b31",
+                "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
+                "testT.freebayes.filtered.bcftools_stats.txt:md5,a5beebaf9e38ab8e3e9c332c46dd9ae1",
+                "testN.strelka.variants.bcftools_stats.txt:md5,fcc53db2f8001b3a68673a9e0802e06c",
+                "testT.strelka.variants.bcftools_stats.txt:md5,d8dd5a46552458e72ea9963e948a377c",
                 "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -338,10 +338,10 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -353,10 +353,10 @@
                     "gatk4": "4.6.1.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GERMLINE_VCFS_CONCAT_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -389,10 +389,10 @@
                     "vcflib": "1.0.14"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -592,10 +592,10 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "testN.freebayes.filtered.bcftools_stats.txt:md5,96c5e6b9e55f1bb67fca7886fb322b67",
-                "testT.freebayes.filtered.bcftools_stats.txt:md5,ce8b7afaace836b6a00bd8ccdc980500",
-                "testN.strelka.variants.bcftools_stats.txt:md5,f11c9e5e9820868809b5465970c7cc06",
-                "testT.strelka.variants.bcftools_stats.txt:md5,ac070b6cf4de7540a227265971244b31",
+                "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
+                "testT.freebayes.filtered.bcftools_stats.txt:md5,a5beebaf9e38ab8e3e9c332c46dd9ae1",
+                "testN.strelka.variants.bcftools_stats.txt:md5,fcc53db2f8001b3a68673a9e0802e06c",
+                "testT.strelka.variants.bcftools_stats.txt:md5,d8dd5a46552458e72ea9963e948a377c",
                 "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -633,7 +633,7 @@
                 "testN.freebayes.filtered.norm.sorted.vcf.gz:md5,18992a755b06d72374bb072cc8af86f9",
                 "testN.strelka.variants.norm.sorted.vcf.gz:md5,ba9aabddec39a8bcbdb38c46f7a26515",
                 "testT.freebayes.filtered.norm.sorted.vcf.gz:md5,574d3d9e73986d07839d5e720c3ea929",
-                "testT.strelka.variants.norm.sorted.vcf.gz:md5,2dd9f7f6dac9c10afcf01f148a486799",
+                "testT.strelka.variants.norm.sorted.vcf.gz:md5,3c0fb37b3523c7f7555e7cc139a1adb1",
                 "testN.strelka.genome.vcf.gz:md5,325ea84ccf1ec1cf7a6c5c4aae1c0d1",
                 "testN.strelka.variants.vcf.gz:md5,3a3b3e67614ec91f4f0b88999dff454b",
                 "testT.strelka.genome.vcf.gz:md5,ece653410947a3ace10296c2cc5ff095",
diff --git a/tests/postprocess_consensus.nf.test.snap b/tests/postprocess_consensus.nf.test.snap
index 4b4dda1067..b6c9b23f00 100644
--- a/tests/postprocess_consensus.nf.test.snap
+++ b/tests/postprocess_consensus.nf.test.snap
@@ -7,16 +7,16 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_CONCAT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_ISEC": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CONSENSUS_FROM_SITES": {
                     "gawk": "mawk 1.3.4 20240123",
@@ -26,7 +26,7 @@
                     "gawk": "5.3.0"
                 },
                 "FILTER_VCFS": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
@@ -62,10 +62,10 @@
                     "tabix": "1.21"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -304,11 +304,11 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.bcftools.bcftools_stats.txt:md5,bd86ded3843a217d429b34edd22a9a4e",
-                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,65bc65858d3dfa1d8913119850626823",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,6d4d032ba146941cb226765aaed9d67f",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample3.bcftools.bcftools_stats.txt:md5,bb4018e2ec62ab957867c4f55ce626b8",
+                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,c802eeeaa4ab6531bdb5d8c0b583f8b6",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
@@ -350,7 +350,7 @@
                 "sample4_vs_sample3.strelka.somatic_indels.bcftools_filtered.vcf.gz:md5,a7861b539e2cfd4429a02757bc9845e9",
                 "sample4_vs_sample3.strelka.somatic_snvs.bcftools_filtered.vcf.gz:md5,973d0ca572b2c94e18279a3c7694d934",
                 "sample4_vs_sample3.mutect2.vcf.gz:md5,f2c46d0dae1b1a59180c0b9e595993d2",
-                "sample3.bcftools.bcftools_filtered.norm.sorted.vcf.gz:md5,ff4832fed7bffc1b53254f635ddb8a95",
+                "sample3.bcftools.bcftools_filtered.norm.sorted.vcf.gz:md5,611a523c501d43f1810f4191267693fb",
                 "sample3.strelka.variants.bcftools_filtered.norm.sorted.vcf.gz:md5,e3f3390321c8693afd777202e15e9b5e",
                 "sample4_vs_sample3.mutect2.bcftools_filtered.norm.sorted.vcf.gz:md5,8826a34d4c7a004750b8a56fee56dcbb",
                 "sample4_vs_sample3.strelka.somatic_indels.bcftools_filtered.norm.sorted.vcf.gz:md5,e60a2a65edfea45253619ccd5c6054f0",
@@ -379,13 +379,13 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_ISEC": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CONSENSUS_FROM_SITES": {
                     "gawk": "mawk 1.3.4 20240123",
@@ -425,10 +425,10 @@
                     "tabix": "1.21"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -613,9 +613,9 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.bcftools.bcftools_stats.txt:md5,3299f97352e32c873c95e43922c79147",
-                "sample2.lofreq.bcftools_stats.txt:md5,a8a850fdd11644fa4b770971dfe37194",
-                "sample2.mutect2.bcftools_stats.txt:md5,846b97f3763c5316f2b7e76962d80be9",
+                "sample2.bcftools.bcftools_stats.txt:md5,47ce340432efdbb38a86d8b7c3d0c2d4",
+                "sample2.lofreq.bcftools_stats.txt:md5,064a0729f34ff2b74a1ea619cc47ecb6",
+                "sample2.mutect2.bcftools_stats.txt:md5,be37ecb07e10f0a3ea082e3fab5226fd",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
@@ -648,7 +648,7 @@
                 ],
                 [
                     "0002.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=55, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=56, phased=false, phasedAutodetect=false]"
                 ],
                 [
                     "sample2.lofreq.vcf.gz",
@@ -660,7 +660,7 @@
                 ],
                 [
                     "sample2.bcftools.norm.sorted.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=132, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=133, phased=false, phasedAutodetect=false]"
                 ],
                 [
                     "sample2.lofreq.norm.sorted.vcf.gz",
@@ -668,7 +668,7 @@
                 ],
                 [
                     "sample2.mutect2.norm.sorted.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=1261, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=1262, phased=false, phasedAutodetect=false]"
                 ]
             ],
             [
@@ -690,16 +690,16 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_CONCAT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_ISEC": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CALCULATECONTAMINATION": {
                     "gatk4": "4.6.1.0"
@@ -754,10 +754,10 @@
                     "tabix": "1.21"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -992,11 +992,11 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.bcftools.bcftools_stats.txt:md5,bd86ded3843a217d429b34edd22a9a4e",
-                "sample4_vs_sample3.mutect2.filtered.bcftools_stats.txt:md5,91e802a68f9d8da14bb3dcf784f0810d",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,6d4d032ba146941cb226765aaed9d67f",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample3.bcftools.bcftools_stats.txt:md5,bb4018e2ec62ab957867c4f55ce626b8",
+                "sample4_vs_sample3.mutect2.filtered.bcftools_stats.txt:md5,ef2f52e9bd9793547bb43a3828f8304c",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
@@ -1038,11 +1038,11 @@
                 ],
                 [
                     "sample3.consensus.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=0, variantCount=70, phased=true, phasedAutodetect=true]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=0, variantCount=71, phased=true, phasedAutodetect=true]"
                 ],
                 [
                     "0001.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=70, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=71, phased=false, phasedAutodetect=false]"
                 ],
                 [
                     "sample4_vs_sample3.consensus.vcf.gz",
@@ -1062,11 +1062,11 @@
                 ],
                 [
                     "sample3.bcftools.norm.sorted.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=123, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=124, phased=false, phasedAutodetect=false]"
                 ],
                 [
                     "sample3.strelka.variants.norm.sorted.vcf.gz",
-                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=52656, phased=false, phasedAutodetect=false]"
+                    "VcfFile [chromosomes=[chr21], sampleCount=1, variantCount=53245, phased=false, phasedAutodetect=false]"
                 ],
                 [
                     "sample4_vs_sample3.mutect2.filtered.norm.sorted.vcf.gz",
diff --git a/tests/postprocess_filtering.nf.test.snap b/tests/postprocess_filtering.nf.test.snap
index 80e7e36a17..bf295ba961 100644
--- a/tests/postprocess_filtering.nf.test.snap
+++ b/tests/postprocess_filtering.nf.test.snap
@@ -4,16 +4,16 @@
             41,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FILTER_VCFS": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "FREEBAYES": {
                     "freebayes": "1.3.10"
@@ -238,10 +238,10 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "testN.freebayes.filtered.bcftools_stats.txt:md5,96c5e6b9e55f1bb67fca7886fb322b67",
-                "testT.freebayes.filtered.bcftools_stats.txt:md5,ce8b7afaace836b6a00bd8ccdc980500",
-                "testN.strelka.variants.bcftools_stats.txt:md5,f11c9e5e9820868809b5465970c7cc06",
-                "testT.strelka.variants.bcftools_stats.txt:md5,ac070b6cf4de7540a227265971244b31",
+                "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
+                "testT.freebayes.filtered.bcftools_stats.txt:md5,a5beebaf9e38ab8e3e9c332c46dd9ae1",
+                "testN.strelka.variants.bcftools_stats.txt:md5,fcc53db2f8001b3a68673a9e0802e06c",
+                "testT.strelka.variants.bcftools_stats.txt:md5,d8dd5a46552458e72ea9963e948a377c",
                 "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
diff --git a/tests/postprocess_normalization.nf.test.snap b/tests/postprocess_normalization.nf.test.snap
index f3744c5d9c..8e019fffdd 100644
--- a/tests/postprocess_normalization.nf.test.snap
+++ b/tests/postprocess_normalization.nf.test.snap
@@ -7,10 +7,10 @@
                     "gawk": "5.3.0"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -52,10 +52,10 @@
                     "vcflib": "1.0.14"
                 },
                 "VCFS_NORM": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFS_NORM_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
                     "vcftools": "0.1.16"
@@ -248,10 +248,10 @@
                 "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
-                "testN.freebayes.filtered.bcftools_stats.txt:md5,96c5e6b9e55f1bb67fca7886fb322b67",
-                "testT.freebayes.filtered.bcftools_stats.txt:md5,ce8b7afaace836b6a00bd8ccdc980500",
-                "testN.strelka.variants.bcftools_stats.txt:md5,f11c9e5e9820868809b5465970c7cc06",
-                "testT.strelka.variants.bcftools_stats.txt:md5,ac070b6cf4de7540a227265971244b31",
+                "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
+                "testT.freebayes.filtered.bcftools_stats.txt:md5,a5beebaf9e38ab8e3e9c332c46dd9ae1",
+                "testN.strelka.variants.bcftools_stats.txt:md5,fcc53db2f8001b3a68673a9e0802e06c",
+                "testT.strelka.variants.bcftools_stats.txt:md5,d8dd5a46552458e72ea9963e948a377c",
                 "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -289,7 +289,7 @@
                 "testN.freebayes.filtered.norm.sorted.vcf.gz:md5,18992a755b06d72374bb072cc8af86f9",
                 "testN.strelka.variants.norm.sorted.vcf.gz:md5,ba9aabddec39a8bcbdb38c46f7a26515",
                 "testT.freebayes.filtered.norm.sorted.vcf.gz:md5,574d3d9e73986d07839d5e720c3ea929",
-                "testT.strelka.variants.norm.sorted.vcf.gz:md5,2dd9f7f6dac9c10afcf01f148a486799",
+                "testT.strelka.variants.norm.sorted.vcf.gz:md5,3c0fb37b3523c7f7555e7cc139a1adb1",
                 "testN.strelka.genome.vcf.gz:md5,325ea84ccf1ec1cf7a6c5c4aae1c0d1",
                 "testN.strelka.variants.vcf.gz:md5,3a3b3e67614ec91f4f0b88999dff454b",
                 "testT.strelka.genome.vcf.gz:md5,ece653410947a3ace10296c2cc5ff095",
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index c52c6f785f..19239f404a 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -10,10 +10,13 @@
                     "varlociraptor": "8.9.5"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CONCAT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
+                },
+                "CONCAT_SOMATIC_STRELKA": {
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -25,7 +28,7 @@
                     "gatk4": "4.6.1.0"
                 },
                 "MERGE_GERMLINE_SOMATIC_VCFS": {
-                    "bcftools": 1.22
+                    "bcftools": "1.23.1"
                 },
                 "MERGE_STRELKA": {
                     "gatk4": "4.6.2.0"
@@ -55,10 +58,10 @@
                     "samtools": 1.21
                 },
                 "SORT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "SORT_FINAL_VCF": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -271,9 +274,9 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,6d4d032ba146941cb226765aaed9d67f",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
@@ -324,13 +327,13 @@
             73,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CALCULATECONTAMINATION": {
                     "gatk4": "4.6.1.0"
                 },
                 "CONCAT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -372,10 +375,10 @@
                     "samtools": 1.21
                 },
                 "SORT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "SORT_FINAL_VCF": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -545,7 +548,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.mutect2.filtered.bcftools_stats.txt:md5,5327cede1f3ad2139945607f66264928",
+                "sample2.mutect2.filtered.bcftools_stats.txt:md5,30c32a41d08f8ae7227521beae37dc62",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
@@ -584,10 +587,10 @@
             68,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CONCAT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -614,10 +617,10 @@
                     "samtools": 1.21
                 },
                 "SORT_CALLED_CHUNKS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "SORT_FINAL_VCF": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -786,7 +789,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.strelka.variants.bcftools_stats.txt:md5,7d091579d450a6f6d6e6ed9795dce0cb",
+                "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
                 "sample1.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample1.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample1.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
diff --git a/tests/qc_ngscheckmate.nf.test.snap b/tests/qc_ngscheckmate.nf.test.snap
index cdaed6601f..bba16b79b9 100644
--- a/tests/qc_ngscheckmate.nf.test.snap
+++ b/tests/qc_ngscheckmate.nf.test.snap
@@ -4,7 +4,7 @@
             17,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -127,7 +127,7 @@
             17,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 6ab3962df8..27a15e889b 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -4,7 +4,7 @@
             29,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -327,7 +327,7 @@
                 "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
-                "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
+                "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
                 "test.md.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
diff --git a/tests/start_from_preparerecalibration.nf.test.snap b/tests/start_from_preparerecalibration.nf.test.snap
index e3926b1dac..c7260b1f8f 100644
--- a/tests/start_from_preparerecalibration.nf.test.snap
+++ b/tests/start_from_preparerecalibration.nf.test.snap
@@ -151,7 +151,7 @@
             10,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -256,7 +256,7 @@
             ],
             [
                 "multiqc_citations.txt:md5,ac2b3cf2dfb12c40837b9bbad8112d86",
-                "test.strelka.variants.bcftools_stats.txt:md5,bffd4c0cf553a42c5b183220d71a1466",
+                "test.strelka.variants.bcftools_stats.txt:md5,e59e1411514a692fddeea663e3d1c3a5",
                 "test.strelka.variants.FILTER.summary:md5,39ff2cc8eb7495a14a6b76e0ab627027",
                 "test.strelka.variants.TsTv.count:md5,ee7dafc8d941b8502a04a63dc3126fff"
             ],
@@ -279,7 +279,7 @@
             10,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -384,7 +384,7 @@
             ],
             [
                 "multiqc_citations.txt:md5,ac2b3cf2dfb12c40837b9bbad8112d86",
-                "test.strelka.variants.bcftools_stats.txt:md5,bffd4c0cf553a42c5b183220d71a1466",
+                "test.strelka.variants.bcftools_stats.txt:md5,e59e1411514a692fddeea663e3d1c3a5",
                 "test.strelka.variants.FILTER.summary:md5,39ff2cc8eb7495a14a6b76e0ab627027",
                 "test.strelka.variants.TsTv.count:md5,ee7dafc8d941b8502a04a63dc3126fff"
             ],
diff --git a/tests/start_from_recalibration.nf.test.snap b/tests/start_from_recalibration.nf.test.snap
index cd6e64394d..4b02d0f806 100644
--- a/tests/start_from_recalibration.nf.test.snap
+++ b/tests/start_from_recalibration.nf.test.snap
@@ -131,7 +131,7 @@
             10,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -236,7 +236,7 @@
             ],
             [
                 "multiqc_citations.txt:md5,ac2b3cf2dfb12c40837b9bbad8112d86",
-                "test.strelka.variants.bcftools_stats.txt:md5,bffd4c0cf553a42c5b183220d71a1466",
+                "test.strelka.variants.bcftools_stats.txt:md5,e59e1411514a692fddeea663e3d1c3a5",
                 "test.strelka.variants.FILTER.summary:md5,39ff2cc8eb7495a14a6b76e0ab627027",
                 "test.strelka.variants.TsTv.count:md5,ee7dafc8d941b8502a04a63dc3126fff"
             ],
@@ -259,7 +259,7 @@
             10,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -364,7 +364,7 @@
             ],
             [
                 "multiqc_citations.txt:md5,ac2b3cf2dfb12c40837b9bbad8112d86",
-                "test.strelka.variants.bcftools_stats.txt:md5,bffd4c0cf553a42c5b183220d71a1466",
+                "test.strelka.variants.bcftools_stats.txt:md5,e59e1411514a692fddeea663e3d1c3a5",
                 "test.strelka.variants.FILTER.summary:md5,39ff2cc8eb7495a14a6b76e0ab627027",
                 "test.strelka.variants.TsTv.count:md5,ee7dafc8d941b8502a04a63dc3126fff"
             ],
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 047e90be95..2c5e8d6bba 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -4,7 +4,7 @@
             40,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -335,9 +335,9 @@
                 "samtools-stats-dp.txt:md5,13dab249f7bef935dfef234299550db8",
                 "samtools_alignment_plot.txt:md5,44149e0c5cc4bfa58242824b300219a2",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
-                "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
-                "test2_vs_test.strelka.somatic_indels.bcftools_stats.txt:md5,5e8f9a8fdbc765ced736d0c8c7dd3a52",
-                "test2_vs_test.strelka.somatic_snvs.bcftools_stats.txt:md5,edb7763fad7b6f825e47e01ffa70adbc",
+                "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
+                "test2_vs_test.strelka.somatic_indels.bcftools_stats.txt:md5,b5d3cad82f19dd86895f5b7b38034bd2",
+                "test2_vs_test.strelka.somatic_snvs.bcftools_stats.txt:md5,08b597e1002f482dc549044c500eb60a",
                 "test.md.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index 352e25861b..dad1469bb4 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -4,7 +4,7 @@
             19,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -230,7 +230,7 @@
                 "samtools-stats-dp.txt:md5,175b0442368f03294ef9ce9e73172c7b",
                 "samtools_alignment_plot.txt:md5,6555bbf8c1ef12058e9a050545a9fa41",
                 "samtools_insert_size.txt:md5,164be68813f2cb96462c152743b95cae",
-                "test.strelka.variants.bcftools_stats.txt:md5,5b7cff7b17c5bcde6177a188b1511986",
+                "test.strelka.variants.bcftools_stats.txt:md5,f4f3355bc1f101411c2fed6cf77d478e",
                 "test.md.mosdepth.global.dist.txt:md5,0c795eadf5e5a8ea8469ba8c0ece9bdd",
                 "test.md.mosdepth.region.dist.txt:md5,54273a37adf55f8cee822aaf92a4c321",
                 "test.md.mosdepth.summary.txt:md5,bb12489d3d44c4e44eb5dcc8fdef636c",
@@ -266,7 +266,7 @@
             30,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -596,7 +596,7 @@
                 "samtools-stats-dp.txt:md5,afe461017b5f2189651f29c79f6fee44",
                 "samtools_alignment_plot.txt:md5,9d6e0d529e64404f2c24e74285e242ee",
                 "samtools_insert_size.txt:md5,91c40430c1296fd8ce04788f2f528754",
-                "test.strelka.variants.bcftools_stats.txt:md5,3f4c40f17e956e4140a0432072a9dde0",
+                "test.strelka.variants.bcftools_stats.txt:md5,a1500be145d3bd8a1e643c8772c4406d",
                 "test.md.mosdepth.global.dist.txt:md5,7b8a7773d5d854ae1b681b971d11bb34",
                 "test.md.mosdepth.region.dist.txt:md5,1dd626f6fef0d1ac29fd7ffb8b1bbd95",
                 "test.md.mosdepth.summary.txt:md5,25af78b074abf710ddd9c90cba1928bc",
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index 4962e9e856..d5c4f474d6 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -4,13 +4,13 @@
             97,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -524,15 +524,15 @@
                 "samtools-stats-dp.txt:md5,13dab249f7bef935dfef234299550db8",
                 "samtools_alignment_plot.txt:md5,44149e0c5cc4bfa58242824b300219a2",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
-                "test.bcftools.bcftools_stats.txt:md5,4b2997064fb500f090639edca1ccaa6e",
-                "test.deepvariant.bcftools_stats.txt:md5,61ab450ed778088b9c879b8dcbbb9db8",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,dde124ceaf6f109cd274b837b950096b",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,189bc07d38c2a9ae34b141cff402bda6",
-                "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
-                "test2_vs_test.strelka.somatic_indels.bcftools_stats.txt:md5,5e8f9a8fdbc765ced736d0c8c7dd3a52",
-                "test2_vs_test.strelka.somatic_snvs.bcftools_stats.txt:md5,edb7763fad7b6f825e47e01ffa70adbc",
-                "test.tiddit.bcftools_stats.txt:md5,2b96446a75e1ec5d5f1310a934c0dd4c",
-                "test2_vs_test.tiddit_sv_merge.bcftools_stats.txt:md5,a6c45b2a2b7d8844e5536deee5c77af5",
+                "test.bcftools.bcftools_stats.txt:md5,9213504c6b04c6c3cdf308ad3e8b4c3a",
+                "test.deepvariant.bcftools_stats.txt:md5,67f779dde7f1c6b6ca5cf48f8c24ba02",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,5b0bc956256ff340d4f7409769fa9924",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,47f9e44efca5ebdc059866e6bfbb0564",
+                "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
+                "test2_vs_test.strelka.somatic_indels.bcftools_stats.txt:md5,b5d3cad82f19dd86895f5b7b38034bd2",
+                "test2_vs_test.strelka.somatic_snvs.bcftools_stats.txt:md5,08b597e1002f482dc549044c500eb60a",
+                "test.tiddit.bcftools_stats.txt:md5,c4cb4e484f95c7dbdaa3d08f0704f7b5",
+                "test2_vs_test.tiddit_sv_merge.bcftools_stats.txt:md5,7c527e6b2eeb8d08c37898a305f32b92",
                 "test.md.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
@@ -646,13 +646,13 @@
             55,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -1068,11 +1068,11 @@
                 "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
-                "test.bcftools.bcftools_stats.txt:md5,4b2997064fb500f090639edca1ccaa6e",
-                "test.deepvariant.bcftools_stats.txt:md5,61ab450ed778088b9c879b8dcbbb9db8",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,c6b6e221504c69ee75b209f4a0b2506a",
-                "test.strelka.variants.bcftools_stats.txt:md5,2613827870dd789fe602a8a3b739b7f2",
-                "test.tiddit.bcftools_stats.txt:md5,2b96446a75e1ec5d5f1310a934c0dd4c",
+                "test.bcftools.bcftools_stats.txt:md5,9213504c6b04c6c3cdf308ad3e8b4c3a",
+                "test.deepvariant.bcftools_stats.txt:md5,67f779dde7f1c6b6ca5cf48f8c24ba02",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,60f55a391db494db3e95bd69b6b36fa3",
+                "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
+                "test.tiddit.bcftools_stats.txt:md5,c4cb4e484f95c7dbdaa3d08f0704f7b5",
                 "test.md.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
@@ -1141,13 +1141,13 @@
             49,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -1539,10 +1539,10 @@
                 "samtools-stats-dp.txt:md5,157a95de8aa4dd8947bb965f0c9ae3c7",
                 "samtools_alignment_plot.txt:md5,46e8824724863d243a01cc239a5eff15",
                 "samtools_insert_size.txt:md5,17c44517c58803f9c078f6d9b55aed00",
-                "test2.bcftools.bcftools_stats.txt:md5,c61cda67fa314ecdeac858599ce732ee",
-                "test2.freebayes.filtered.bcftools_stats.txt:md5,57082cdcbbe9ffb4c63b314912227afb",
-                "test2.mutect2.filtered.bcftools_stats.txt:md5,3ecb2b3bb668d9f808787ffaa780fc03",
-                "test2.tiddit.bcftools_stats.txt:md5,1122042610f0aed654c8d8b817fd1c50",
+                "test2.bcftools.bcftools_stats.txt:md5,fa4bd52259a3400e5d21c43b570e40a5",
+                "test2.freebayes.filtered.bcftools_stats.txt:md5,9bb83f7b4cad18474e47b5cee9160a32",
+                "test2.mutect2.filtered.bcftools_stats.txt:md5,cb2c8f2f4b0ceee60fb1243191d4f429",
+                "test2.tiddit.bcftools_stats.txt:md5,346588891b9ab59af56f44649421b424",
                 "test2.md.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.md.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.md.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
diff --git a/tests/variant_calling_deepvariant.nf.test.snap b/tests/variant_calling_deepvariant.nf.test.snap
index f109166849..916214ce27 100644
--- a/tests/variant_calling_deepvariant.nf.test.snap
+++ b/tests/variant_calling_deepvariant.nf.test.snap
@@ -4,7 +4,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -161,7 +161,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.deepvariant.bcftools_stats.txt:md5,a19ff85c0bc9796ace876f3a9c34c6ce",
+                "test.deepvariant.bcftools_stats.txt:md5,c784614aec9ed43f1d4a6149c8ae8bf3",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -189,7 +189,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -340,7 +340,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "DEEPVARIANT_RUNDEEPVARIANT": {
                     "deepvariant": "1.10.0"
@@ -484,7 +484,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "DEEPVARIANT_RUNDEEPVARIANT": {
                     "deepvariant": "1.10.0"
@@ -634,7 +634,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.deepvariant.bcftools_stats.txt:md5,a19ff85c0bc9796ace876f3a9c34c6ce",
+                "test.deepvariant.bcftools_stats.txt:md5,c784614aec9ed43f1d4a6149c8ae8bf3",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 79e58083b4..0b09f6ebd9 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -4,10 +4,10 @@
             19,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -180,7 +180,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.freebayes.filtered.bcftools_stats.txt:md5,58e57fe87d5eb8b7ccd9b1ecd2d196df",
+                "sample2.freebayes.filtered.bcftools_stats.txt:md5,8d125501aef859ecb64b7363d93429fe",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
@@ -215,10 +215,10 @@
             41,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -554,8 +554,8 @@
                 "samtools-stats-dp.txt:md5,7a0481b59cdd57cc0b8bc9b5641614c6",
                 "samtools_alignment_plot.txt:md5,8e6178a26fe2a4fc4f45fac3175ba6c6",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,dde124ceaf6f109cd274b837b950096b",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,189bc07d38c2a9ae34b141cff402bda6",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,5b0bc956256ff340d4f7409769fa9924",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,47f9e44efca5ebdc059866e6bfbb0564",
                 "test.md.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.md.mosdepth.summary.txt:md5,0010c2396a3173c7cf4983abe2eb6a4c",
                 "test.md.per-base.bed.gz:md5,34dfe443c0a0767562dd65272e3310ef",
@@ -620,10 +620,10 @@
             33,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -942,7 +942,7 @@
                 "samtools-stats-dp.txt:md5,300b95526a211b05f18aaffd037dacd3",
                 "samtools_alignment_plot.txt:md5,5053f650b8612fe5e8527b0bca8ab905",
                 "samtools_insert_size.txt:md5,2b1da7c476290081135a2816f4231333",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,5ad7fc8a51dd1dc0f827a46acbed01c4",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,b0d8c9234a3b038c05fd14d656e43956",
                 "test.md.mosdepth.global.dist.txt:md5,531a83245143e7975f18e1988c876138",
                 "test.md.mosdepth.region.dist.txt:md5,d25723bdd3fec6b17d2462abfa097b9e",
                 "test.md.mosdepth.summary.txt:md5,87be70cd1237d7af9aa40d8cd8b3a817",
@@ -989,10 +989,10 @@
             24,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -1297,7 +1297,7 @@
                 "samtools-stats-dp.txt:md5,9e836f09043529495de9e1c268ee8e82",
                 "samtools_alignment_plot.txt:md5,bcc2f176a4bc51b33a36bd4381f048a5",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,c6b6e221504c69ee75b209f4a0b2506a",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,60f55a391db494db3e95bd69b6b36fa3",
                 "test.md.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
@@ -1340,10 +1340,10 @@
             14,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "FREEBAYES": {
                     "freebayes": "1.3.10"
@@ -1509,7 +1509,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.freebayes.filtered.bcftools_stats.txt:md5,b83440f3699a3258015d0ce60eea0d46",
+                "sample2.freebayes.filtered.bcftools_stats.txt:md5,6bc59a78f70ac18e8d69a779a7892673",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
@@ -1541,10 +1541,10 @@
             56,
             {
                 "BCFTOOLS_SORT": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.18-r1243-dirty"
@@ -1902,8 +1902,8 @@
                 "samtools-stats-dp.txt:md5,ff7906db0da2f9c9ba5ce85c34694124",
                 "samtools_alignment_plot.txt:md5,89d0a6e7076223e9feadbecd794948d5",
                 "samtools_insert_size.txt:md5,ca54b785b1d63edd61cef58cd5558aa2",
-                "test.freebayes.filtered.bcftools_stats.txt:md5,b313075b3e9854e0b561dd8d754bf097",
-                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,42d0644fc84df43b26e8de1ad427b446",
+                "test.freebayes.filtered.bcftools_stats.txt:md5,49399853673a44189a06f171efb1c670",
+                "test2_vs_test.freebayes.filtered.bcftools_stats.txt:md5,7d4efbffe37f0a3dad729361353d32a9",
                 "test.md.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.md.mosdepth.region.dist.txt:md5,835fdc6fa52cc33e6fb76c0c20a8a6c3",
                 "test.md.mosdepth.summary.txt:md5,dcc9ab2bf3248903e02d8da87e678977",
diff --git a/tests/variant_calling_haplotypecaller.nf.test.snap b/tests/variant_calling_haplotypecaller.nf.test.snap
index e48ae8904b..fefcdc20f0 100644
--- a/tests/variant_calling_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_haplotypecaller.nf.test.snap
@@ -4,7 +4,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GATK4_HAPLOTYPECALLER": {
                     "gatk4": "4.6.1.0"
@@ -155,7 +155,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotypecaller.bcftools_stats.txt:md5,1497941f37b14c39a24490a50a97e365",
+                "test.haplotypecaller.bcftools_stats.txt:md5,b9b8c3cb422534bdb7a45d884c121af8",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.summary.txt:md5,4f0d231060cbde4efdd673863bd2fb59",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
@@ -181,7 +181,7 @@
             14,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -342,7 +342,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotypecaller.bcftools_stats.txt:md5,1497941f37b14c39a24490a50a97e365",
+                "test.haplotypecaller.bcftools_stats.txt:md5,b9b8c3cb422534bdb7a45d884c121af8",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "test.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
@@ -371,7 +371,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CNNSCOREVARIANTS": {
                     "gatk4": "4.5.0.0"
@@ -530,7 +530,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotypecaller.filtered.bcftools_stats.txt:md5,bfdbcc0c0513be1e223434eefee3b90b",
+                "test.haplotypecaller.filtered.bcftools_stats.txt:md5,5debbaa917411dda07c7972272a95f38",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.summary.txt:md5,4f0d231060cbde4efdd673863bd2fb59",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
@@ -557,7 +557,7 @@
             16,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CNNSCOREVARIANTS": {
                     "gatk4": "4.5.0.0"
@@ -726,7 +726,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotypecaller.filtered.bcftools_stats.txt:md5,bfdbcc0c0513be1e223434eefee3b90b",
+                "test.haplotypecaller.filtered.bcftools_stats.txt:md5,5debbaa917411dda07c7972272a95f38",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "test.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
diff --git a/tests/variant_calling_lofreq.nf.test.snap b/tests/variant_calling_lofreq.nf.test.snap
index d53a63c2d0..8e641e9b68 100644
--- a/tests/variant_calling_lofreq.nf.test.snap
+++ b/tests/variant_calling_lofreq.nf.test.snap
@@ -4,7 +4,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -158,7 +158,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.lofreq.bcftools_stats.txt:md5,a8a850fdd11644fa4b770971dfe37194",
+                "sample2.lofreq.bcftools_stats.txt:md5,064a0729f34ff2b74a1ea619cc47ecb6",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
@@ -189,7 +189,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
@@ -336,7 +336,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.lofreq.bcftools_stats.txt:md5,dd602205b6d368eb0e21d2a94c36e0de",
+                "sample2.lofreq.bcftools_stats.txt:md5,1aa21f81ba86f60f145934ab517fc44e",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
diff --git a/tests/variant_calling_manta.nf.test.snap b/tests/variant_calling_manta.nf.test.snap
index 129517d0c9..b5b032807a 100644
--- a/tests/variant_calling_manta.nf.test.snap
+++ b/tests/variant_calling_manta.nf.test.snap
@@ -4,7 +4,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -130,7 +130,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,636109db283cbee4539786928c811893",
+                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -157,7 +157,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MANTA_TUMORONLY": {
                     "manta": "1.6.0"
@@ -279,7 +279,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,9fbe26c75869000b526b59b454f76f6a",
+                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,6a4c5f596d407e12449c36fe3419488f",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
@@ -306,7 +306,7 @@
             20,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MANTA_GERMLINE": {
                     "manta": "1.6.0"
@@ -454,9 +454,9 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,36a838390faba81e3eabf5ac8a093a4a",
-                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,f00cf810d34ef7e5c7980f7039bb4446",
-                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7af2ea2e84154ddf2a483b1bd1f0646c",
+                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
+                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,7be348c7ec03195c429772b65d768d10",
+                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7d7b08cfb7b97ef4949d909d3ed04e88",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -494,7 +494,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -620,7 +620,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,9fbe26c75869000b526b59b454f76f6a",
+                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,6a4c5f596d407e12449c36fe3419488f",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
@@ -647,7 +647,7 @@
             31,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -827,10 +827,10 @@
                 "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
-                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,636109db283cbee4539786928c811893",
-                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,9fbe26c75869000b526b59b454f76f6a",
-                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,f00cf810d34ef7e5c7980f7039bb4446",
-                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7af2ea2e84154ddf2a483b1bd1f0646c",
+                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
+                "sample2.manta.tumor_sv.bcftools_stats.txt:md5,6a4c5f596d407e12449c36fe3419488f",
+                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,7be348c7ec03195c429772b65d768d10",
+                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7d7b08cfb7b97ef4949d909d3ed04e88",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -881,7 +881,7 @@
             22,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -1033,9 +1033,9 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,36a838390faba81e3eabf5ac8a093a4a",
-                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,f00cf810d34ef7e5c7980f7039bb4446",
-                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7af2ea2e84154ddf2a483b1bd1f0646c",
+                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
+                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,7be348c7ec03195c429772b65d768d10",
+                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7d7b08cfb7b97ef4949d909d3ed04e88",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -1073,7 +1073,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MANTA_GERMLINE": {
                     "manta": "1.6.0"
@@ -1195,7 +1195,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,636109db283cbee4539786928c811893",
+                "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
diff --git a/tests/variant_calling_mpileup.nf.test.snap b/tests/variant_calling_mpileup.nf.test.snap
index 2df749fd35..9cf0aebfc4 100644
--- a/tests/variant_calling_mpileup.nf.test.snap
+++ b/tests/variant_calling_mpileup.nf.test.snap
@@ -4,10 +4,10 @@
             11,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -160,7 +160,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.bcftools.bcftools_stats.txt:md5,3299f97352e32c873c95e43922c79147",
+                "sample2.bcftools.bcftools_stats.txt:md5,47ce340432efdbb38a86d8b7c3d0c2d4",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
@@ -187,10 +187,10 @@
             9,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -339,7 +339,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.bcftools.bcftools_stats.txt:md5,0659e2f55ea631b8757dd04facc286a1",
+                "sample1.bcftools.bcftools_stats.txt:md5,81ab4ccc0da202a814b7f99ba5c49bb4",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -366,10 +366,10 @@
             9,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -518,7 +518,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.bcftools.bcftools_stats.txt:md5,cc6063baaf7443b12b3fa1c972e804c8",
+                "sample2.bcftools.bcftools_stats.txt:md5,4897ad81224f076a9d7054ba865de378",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
@@ -545,10 +545,10 @@
             11,
             {
                 "BCFTOOLS_MPILEUP": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -701,7 +701,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.bcftools.bcftools_stats.txt:md5,a4865cc7e9dfbea42d098f4bbbc7459d",
+                "sample1.bcftools.bcftools_stats.txt:md5,51bc17eed97a82f7a4a59d5f8181a1cc",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index 15c1b55ede..682969c7b4 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -4,7 +4,7 @@
             17,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": 1.21
@@ -178,7 +178,7 @@
             18,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": 1.21
@@ -328,7 +328,7 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample4_vs_sample3.muse.bcftools_stats.txt:md5,09a0d72425a3638cbe8f1cbd254e66f3",
+                "sample4_vs_sample3.muse.bcftools_stats.txt:md5,78caff99ab648b7191fd74954713aef9",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index c67f562fc4..1046b8b134 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -4,7 +4,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
@@ -161,7 +161,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.mutect2.bcftools_stats.txt:md5,c275ee76762a37053d43f4e290485af8",
+                "sample2.mutect2.bcftools_stats.txt:md5,634af596519997cd802fbc8fe290342f",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
@@ -191,7 +191,7 @@
             15,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -365,7 +365,7 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,65bc65858d3dfa1d8913119850626823",
+                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,c802eeeaa4ab6531bdb5d8c0b583f8b6",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
@@ -405,7 +405,7 @@
             13,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
                     "gatk4": "4.6.1.0"
@@ -569,7 +569,7 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,65bc65858d3dfa1d8913119850626823",
+                "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,c802eeeaa4ab6531bdb5d8c0b583f8b6",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.summary.txt:md5,d2775eb102acc5950f7f53883dcb503d",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
@@ -603,7 +603,7 @@
             14,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "BUILD_INTERVALS": {
                     "gawk": "5.3.0"
@@ -770,7 +770,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.mutect2.bcftools_stats.txt:md5,c275ee76762a37053d43f4e290485af8",
+                "sample2.mutect2.bcftools_stats.txt:md5,634af596519997cd802fbc8fe290342f",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,b0b47739dcafeeb1a9e6218b8abca1e0",
diff --git a/tests/variant_calling_sentieon_dnascope.nf.test.snap b/tests/variant_calling_sentieon_dnascope.nf.test.snap
index 8b3c2ac350..4b4aa56f68 100644
--- a/tests/variant_calling_sentieon_dnascope.nf.test.snap
+++ b/tests/variant_calling_sentieon_dnascope.nf.test.snap
@@ -4,7 +4,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -163,7 +163,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.dnascope.unfiltered.bcftools_stats.txt:md5,ccd33b2c34ef09efe1fd7a86474eaa53",
+                "test.dnascope.unfiltered.bcftools_stats.txt:md5,e7e014ba2764fca171a3291c26cca34c",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -190,7 +190,7 @@
             13,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -354,7 +354,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.dnascope.filtered.bcftools_stats.txt:md5,ad4472e0b4767e0f3c4052ff3e3c1cdb",
+                "test.dnascope.filtered.bcftools_stats.txt:md5,af2789c5de131bbd90b133b9382f506f",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -381,8 +381,11 @@
         "content": [
             15,
             {
+                "BCFTOOLS_SORT": {
+                    "bcftools": "1.23.1"
+                },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -550,7 +553,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "joint_germline.bcftools_stats.txt:md5,b66f04fbf0ca30785d07a4d0b4156bb7",
+                "joint_germline.bcftools_stats.txt:md5,936ca7dc4a8d81cb73a9446057ec4b24",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
diff --git a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
index d1d0391e98..972acd6171 100644
--- a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
@@ -3,8 +3,11 @@
         "content": [
             19,
             {
+                "BCFTOOLS_SORT": {
+                    "bcftools": "1.23.1"
+                },
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -186,7 +189,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "joint_germline_recalibrated_indel.bcftools_stats.txt:md5,f223948705dd87d2edcc6a44ca72df64",
+                "joint_germline_recalibrated_indel.bcftools_stats.txt:md5,f87581b827c876e433e51e39342379ca",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -217,7 +220,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CNNSCOREVARIANTS": {
                     "gatk4": "4.5.0.0"
@@ -377,7 +380,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotyper.filtered.bcftools_stats.txt:md5,178ef0aeaef94b9de01a44d833823918",
+                "test.haplotyper.filtered.bcftools_stats.txt:md5,f4863054921218b7821858f5836029e4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -405,7 +408,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -564,7 +567,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotyper.unfiltered.bcftools_stats.txt:md5,a486e123a1466777f49bab8c854f0e55",
+                "test.haplotyper.unfiltered.bcftools_stats.txt:md5,69874476b2830366fff35b242dbfdfa5",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
@@ -591,7 +594,7 @@
             14,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CNNSCOREVARIANTS": {
                     "gatk4": "4.5.0.0"
@@ -758,7 +761,7 @@
                 "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
-                "test.haplotyper.filtered.bcftools_stats.txt:md5,178ef0aeaef94b9de01a44d833823918",
+                "test.haplotyper.filtered.bcftools_stats.txt:md5,f4863054921218b7821858f5836029e4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
diff --git a/tests/variant_calling_strelka.nf.test.snap b/tests/variant_calling_strelka.nf.test.snap
index faba08c70a..058077e09f 100644
--- a/tests/variant_calling_strelka.nf.test.snap
+++ b/tests/variant_calling_strelka.nf.test.snap
@@ -4,7 +4,7 @@
             22,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -188,9 +188,9 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,6d4d032ba146941cb226765aaed9d67f",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -229,7 +229,7 @@
             11,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -383,7 +383,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.strelka.variants.bcftools_stats.txt:md5,7d091579d450a6f6d6e6ed9795dce0cb",
+                "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -411,7 +411,7 @@
             26,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -611,9 +611,9 @@
                 "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
-                "sample1.strelka.variants.bcftools_stats.txt:md5,7d091579d450a6f6d6e6ed9795dce0cb",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -662,7 +662,7 @@
             9,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -812,7 +812,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.strelka.variants.bcftools_stats.txt:md5,a125b261633ee5e73c4c0bfead86c77c",
+                "sample1.strelka.variants.bcftools_stats.txt:md5,198d97ab341f78b4c33ee345ebdfe5e2",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -840,7 +840,7 @@
             20,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -1020,9 +1020,9 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,322c544c624565a9ab0e128bca556d81",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,eec9d410eb24068b9a67be417c41d54e",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,9a87ae32f97121c0e033043cd6605f67",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,3cc8ac7236cc77f857f6e56a3efa27f5",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
diff --git a/tests/variant_calling_strelka_bp.nf.test.snap b/tests/variant_calling_strelka_bp.nf.test.snap
index b5c6c1d974..043b22d8fe 100644
--- a/tests/variant_calling_strelka_bp.nf.test.snap
+++ b/tests/variant_calling_strelka_bp.nf.test.snap
@@ -4,7 +4,7 @@
             34,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "MANTA_GERMLINE": {
                     "manta": "1.6.0"
@@ -217,12 +217,12 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,36a838390faba81e3eabf5ac8a093a4a",
-                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,f00cf810d34ef7e5c7980f7039bb4446",
-                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7af2ea2e84154ddf2a483b1bd1f0646c",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,322c544c624565a9ab0e128bca556d81",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,eec9d410eb24068b9a67be417c41d54e",
+                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
+                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,7be348c7ec03195c429772b65d768d10",
+                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7d7b08cfb7b97ef4949d909d3ed04e88",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,9a87ae32f97121c0e033043cd6605f67",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,3cc8ac7236cc77f857f6e56a3efa27f5",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -270,7 +270,7 @@
             36,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -487,12 +487,12 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,36a838390faba81e3eabf5ac8a093a4a",
-                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,f00cf810d34ef7e5c7980f7039bb4446",
-                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7af2ea2e84154ddf2a483b1bd1f0646c",
-                "sample3.strelka.variants.bcftools_stats.txt:md5,6d4d032ba146941cb226765aaed9d67f",
-                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,62c6123f6494c3cdbd42dc7230e757b3",
-                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,8404ea88658fbc41d447ba20bf46dd0a",
+                "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
+                "sample4_vs_sample3.manta.diploid_sv.bcftools_stats.txt:md5,7be348c7ec03195c429772b65d768d10",
+                "sample4_vs_sample3.manta.somatic_sv.bcftools_stats.txt:md5,7d7b08cfb7b97ef4949d909d3ed04e88",
+                "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
+                "sample4_vs_sample3.strelka.somatic_indels.bcftools_stats.txt:md5,0092bfd1833160732c4e3180a2a8d54d",
+                "sample4_vs_sample3.strelka.somatic_snvs.bcftools_stats.txt:md5,91f318ab5e6b017554f8f72866c6a5bd",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
diff --git a/tests/variant_calling_tiddit.nf.test.snap b/tests/variant_calling_tiddit.nf.test.snap
index ddcd96719f..40c6991379 100644
--- a/tests/variant_calling_tiddit.nf.test.snap
+++ b/tests/variant_calling_tiddit.nf.test.snap
@@ -4,7 +4,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -135,7 +135,7 @@
                 "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
-                "sample1.tiddit.bcftools_stats.txt:md5,ee25406b8d3ed73eaa8a66972c805c1f",
+                "sample1.tiddit.bcftools_stats.txt:md5,ce8d4ce1c8fde22cf1557603caa1c4e9",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
@@ -162,7 +162,7 @@
             12,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -293,7 +293,7 @@
                 "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
-                "sample2.tiddit.bcftools_stats.txt:md5,82343c0b28dace889f164bbb256a8461",
+                "sample2.tiddit.bcftools_stats.txt:md5,f49030d75a44dde7a9d068af09e89c52",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
@@ -320,7 +320,7 @@
             23,
             {
                 "BCFTOOLS_STATS": {
-                    "bcftools": 1.21
+                    "bcftools": "1.23.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -476,8 +476,8 @@
                 "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
-                "sample3.tiddit.bcftools_stats.txt:md5,b8a60370884c8f2c94baa7d3e859492f",
-                "sample4_vs_sample3.tiddit_sv_merge.bcftools_stats.txt:md5,82f123b157211ac9b8d3b145bbea2147",
+                "sample3.tiddit.bcftools_stats.txt:md5,981a4170dd5a6bd6e5f51bbb169f8a17",
+                "sample4_vs_sample3.tiddit_sv_merge.bcftools_stats.txt:md5,6d73929bb5b17c93f98eed8db6ac7bc4",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index e310725ea8..324c396371 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -273,7 +273,7 @@ workflow SAREK {
     CRAM_SAMPLEQC(
         cram_variant_calling,
         ngscheckmate_bed,
-        fasta,
+        fasta.combine(fasta_fai).map { meta_fasta_, fasta_file , _meta_fai, fai -> [meta_fasta_, fasta_file, fai] }.collect(),
         skip_tools.split(',').contains('baserecalibrator'),
         intervals_for_preprocessing,
     )

From 3c7da70814ab7e8ec4996425f0276ecd23fb4272 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Thu, 16 Jul 2026 12:58:42 +0200
Subject: [PATCH 09/27] Migrate local code to lowercase `channel` factory
 (#2232)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Part of preparing nf-core/sarek for the Nextflow **26.04/26.10**
strict-syntax parser. The uppercase `Channel.` factory is deprecated in
favour of the lowercase `channel.` factory.

This PR replaces **all 438 uppercase `Channel.` occurrences across 42
local files** (`main.nf`, `workflows/`, `subworkflows/local/`,
`modules/local/`, incl. `.nf.test` files) with lowercase `channel.`.

- Pure mechanical casing change — verified that every added line is
identical to its removed counterpart after normalising `Channel.` →
`channel.` (no collateral edits).
- Channel semantics are unchanged; `channel.empty/of/value/fromPath`
behave identically to their uppercase forms.
- Vendored `modules/nf-core/` and `subworkflows/nf-core/` are **not**
touched (they come via `nf-core ... update`).

### Follow-ups (not in this PR, to keep it single-purpose)

Other strict-syntax items surfaced during the audit, tracked separately:

- `channel.from` → `channel.of` at
`subworkflows/local/prepare_genome/main.nf` (`from` is itself
deprecated; casing was applied here but the operator still needs
changing).
- Implicit closure params (`{ it.foo }` → `{ v -> v.foo }`) — ~29
occurrences.
- Remove deprecated `nextflow.enable.configProcessNamesValidation` flag
in `nextflow.config`.
- `workflow.onComplete`/`onError` in the utils subworkflow — will come
via nf-core template sync.

## PR checklist

- [x] This comment contains a description of changes (with reason).
- [x] `CHANGELOG.md` is updated.
- [ ] Make sure your code lints (`nf-core pipelines lint`) — pre-commit
(prettier) passes locally; full lint/nf-test left to CI.
- [ ] Ensure the test suite passes — deferred to CI (mechanical change,
semantically equivalent).

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
---
 CHANGELOG.md                                  |   1 +
 .../local/bam_baserecalibrator/main.nf        |   2 +-
 .../local/bam_baserecalibrator_spark/main.nf  |   2 +-
 .../local/bam_convert_samtools/main.nf        |   2 +-
 .../bam_joint_calling_germline_gatk/main.nf   |   2 +-
 .../main.nf                                   |   2 +-
 subworkflows/local/bam_markduplicates/main.nf |   4 +-
 .../local/bam_markduplicates_spark/main.nf    |   4 +-
 .../local/bam_merge_index_samtools/main.nf    |   2 +-
 subworkflows/local/bam_sentieon_dedup/main.nf |   4 +-
 .../local/bam_variant_calling_cnvkit/main.nf  |   2 +-
 .../bam_variant_calling_deepvariant/main.nf   |   6 +-
 .../bam_variant_calling_freebayes/main.nf     |   2 +-
 .../bam_variant_calling_germline_all/main.nf  |  50 +--
 .../main.nf                                   |   2 +-
 .../main.nf                                   |  10 +-
 .../bam_variant_calling_indexcov/main.nf      |   2 +-
 .../local/bam_variant_calling_mpileup/main.nf |   2 +-
 .../main.nf                                   |  16 +-
 .../main.nf                                   |  16 +-
 .../main.nf                                   |   6 +-
 .../bam_variant_calling_somatic_ascat/main.nf |   2 +-
 .../bam_variant_calling_somatic_manta/main.nf |   6 +-
 .../main.nf                                   |  20 +-
 .../main.nf                                   |   6 +-
 .../main.nf                                   |   2 +-
 .../main.nf                                   |   6 +-
 .../main.nf                                   |  36 +-
 .../main.nf                                   |   6 +-
 .../main.nf                                   |   2 +-
 .../main.nf                                   |  18 +-
 .../main.nf                                   |   6 +-
 .../local/cram_qc_mosdepth_samtools/main.nf   |   4 +-
 .../post_variantcalling/tests/main.nf.test    | 350 +++++++++---------
 subworkflows/local/prepare_genome/main.nf     |  90 ++---
 .../prepare_genome/tests/bbsplit.nf.test      | 130 +++----
 subworkflows/local/prepare_intervals/main.nf  |  16 +-
 .../local/prepare_reference_cnvkit/main.nf    |   2 +-
 .../samplesheet_to_channel/tests/main.nf.test |   2 +-
 subworkflows/local/vcf_consensus/main.nf      |   2 +-
 .../local/vcf_qc_bcftools_vcftools/main.nf    |   2 +-
 .../local/vcf_variant_filtering_gatk/main.nf  |   2 +-
 42 files changed, 425 insertions(+), 424 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 118d50347a..f4e56870f5 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -15,6 +15,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - [#2210](https://github.com/nf-core/sarek/pull/2210) - Update bcftools to 1.23.1
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update EnsemblVEP to 116.0
+- [#2232](https://github.com/nf-core/sarek/pull/2232) - Migrate local code to the lowercase `channel` factory for Nextflow strict-syntax / 26.x readiness
 
 ### Fixed
 
diff --git a/subworkflows/local/bam_baserecalibrator/main.nf b/subworkflows/local/bam_baserecalibrator/main.nf
index 9535c6463e..70563ab461 100644
--- a/subworkflows/local/bam_baserecalibrator/main.nf
+++ b/subworkflows/local/bam_baserecalibrator/main.nf
@@ -18,7 +18,7 @@ workflow BAM_BASERECALIBRATOR {
     known_sites_tbi // channel: [optional]  [ known_sites_tbi ]
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
diff --git a/subworkflows/local/bam_baserecalibrator_spark/main.nf b/subworkflows/local/bam_baserecalibrator_spark/main.nf
index 98f597ff43..26a881b313 100644
--- a/subworkflows/local/bam_baserecalibrator_spark/main.nf
+++ b/subworkflows/local/bam_baserecalibrator_spark/main.nf
@@ -18,7 +18,7 @@ workflow BAM_BASERECALIBRATOR_SPARK {
     known_sites_tbi // channel: [optional]  [ known_sites_tbi ]
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
diff --git a/subworkflows/local/bam_convert_samtools/main.nf b/subworkflows/local/bam_convert_samtools/main.nf
index c20001d087..d101f34b45 100644
--- a/subworkflows/local/bam_convert_samtools/main.nf
+++ b/subworkflows/local/bam_convert_samtools/main.nf
@@ -19,7 +19,7 @@ workflow BAM_CONVERT_SAMTOOLS {
     interleaved // value: true/false
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Index File if not PROVIDED -> this also requires updates to samtools view possibly URGH
 
diff --git a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
index ef4a4426ed..d7d04fc094 100644
--- a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
+++ b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
@@ -31,7 +31,7 @@ workflow BAM_JOINT_CALLING_GERMLINE_GATK {
     known_snps_vqsr
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Map input for GenomicsDBImport
     // Rename based on num_intervals, group all samples by their interval_name/interval_file and restructure for channel
diff --git a/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf b/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
index 3816a2470f..a364876c6b 100644
--- a/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
+++ b/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
@@ -30,7 +30,7 @@ workflow BAM_JOINT_CALLING_GERMLINE_SENTIEON {
     variant_caller
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     sentieon_input = input
         .map{ meta, gvcf, tbi, intervals -> [ [ id:'joint_variant_calling', intervals_name:intervals.baseName, num_intervals:meta.num_intervals ], gvcf, tbi, intervals ] }
diff --git a/subworkflows/local/bam_markduplicates/main.nf b/subworkflows/local/bam_markduplicates/main.nf
index cd9973b837..baed8ec73e 100644
--- a/subworkflows/local/bam_markduplicates/main.nf
+++ b/subworkflows/local/bam_markduplicates/main.nf
@@ -15,8 +15,8 @@ workflow BAM_MARKDUPLICATES {
     intervals_bed_combined // channel: [optional]  [ intervals_bed ]
 
     main:
-    versions = Channel.empty()
-    reports  = Channel.empty()
+    versions = channel.empty()
+    reports  = channel.empty()
 
     // RUN MARKUPDUPLICATES
     // --CREATE_INDEX true is set via ext.args when --save_output_as_bam, so the
diff --git a/subworkflows/local/bam_markduplicates_spark/main.nf b/subworkflows/local/bam_markduplicates_spark/main.nf
index cc0a7167ce..e897b6088b 100644
--- a/subworkflows/local/bam_markduplicates_spark/main.nf
+++ b/subworkflows/local/bam_markduplicates_spark/main.nf
@@ -18,8 +18,8 @@ workflow BAM_MARKDUPLICATES_SPARK {
     intervals_bed_combined        // channel: [optional]  intervals_bed
 
     main:
-    versions = Channel.empty()
-    reports = Channel.empty()
+    versions = channel.empty()
+    reports = channel.empty()
 
     // RUN MARKUPDUPLICATES SPARK
     GATK4SPARK_MARKDUPLICATES(bam, fasta.map{ meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ meta, dict_ -> [ dict_ ] })
diff --git a/subworkflows/local/bam_merge_index_samtools/main.nf b/subworkflows/local/bam_merge_index_samtools/main.nf
index d8b1657a00..61d252d226 100644
--- a/subworkflows/local/bam_merge_index_samtools/main.nf
+++ b/subworkflows/local/bam_merge_index_samtools/main.nf
@@ -12,7 +12,7 @@ workflow BAM_MERGE_INDEX_SAMTOOLS {
     bam // channel: [mandatory] meta, bam
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Figuring out if there is one or more bam(s) from the same sample
     bam_to_merge = bam.branch{ meta, bam_ ->
diff --git a/subworkflows/local/bam_sentieon_dedup/main.nf b/subworkflows/local/bam_sentieon_dedup/main.nf
index b15f7dc32b..33ffffd79d 100644
--- a/subworkflows/local/bam_sentieon_dedup/main.nf
+++ b/subworkflows/local/bam_sentieon_dedup/main.nf
@@ -13,8 +13,8 @@ workflow BAM_SENTIEON_DEDUP {
     intervals_bed_combined // channel: [optional]  [ intervals_bed ]
 
     main:
-    versions = Channel.empty()
-    reports  = Channel.empty()
+    versions = channel.empty()
+    reports  = channel.empty()
 
     bam = bam.map{ meta, bam_ -> [ meta - meta.subMap('data_type'), bam_ ] }
     bai = bai.map{ meta, bai_ -> [ meta - meta.subMap('data_type'), bai_ ] }
diff --git a/subworkflows/local/bam_variant_calling_cnvkit/main.nf b/subworkflows/local/bam_variant_calling_cnvkit/main.nf
index 20d0fe3fd1..2567f0042c 100644
--- a/subworkflows/local/bam_variant_calling_cnvkit/main.nf
+++ b/subworkflows/local/bam_variant_calling_cnvkit/main.nf
@@ -18,7 +18,7 @@ workflow BAM_VARIANT_CALLING_CNVKIT {
     reference           // channel: [optional]  meta, cnn
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
     generate_pon = false
 
     CNVKIT_BATCH(cram, fasta, fasta_fai, targets, reference, generate_pon)
diff --git a/subworkflows/local/bam_variant_calling_deepvariant/main.nf b/subworkflows/local/bam_variant_calling_deepvariant/main.nf
index aadb816710..9047358866 100644
--- a/subworkflows/local/bam_variant_calling_deepvariant/main.nf
+++ b/subworkflows/local/bam_variant_calling_deepvariant/main.nf
@@ -55,16 +55,16 @@ workflow BAM_VARIANT_CALLING_DEEPVARIANT {
     }
 
     // Mix intervals and no_intervals channels together
-    gvcf = Channel.empty().mix(MERGE_DEEPVARIANT_GVCF.out.vcf, gvcf_out.no_intervals)
+    gvcf = channel.empty().mix(MERGE_DEEPVARIANT_GVCF.out.vcf, gvcf_out.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'deepvariant' ], vcf ] }
 
     // Mix intervals and no_intervals channels together
-    vcf = Channel.empty().mix(MERGE_DEEPVARIANT_VCF.out.vcf, vcf_out.no_intervals)
+    vcf = channel.empty().mix(MERGE_DEEPVARIANT_VCF.out.vcf, vcf_out.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'deepvariant' ], vcf ] }
 
-    tbi = Channel.empty().mix(MERGE_DEEPVARIANT_VCF.out.tbi, tbi_out.no_intervals)
+    tbi = channel.empty().mix(MERGE_DEEPVARIANT_VCF.out.tbi, tbi_out.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'deepvariant' ], tbi ] }
 
diff --git a/subworkflows/local/bam_variant_calling_freebayes/main.nf b/subworkflows/local/bam_variant_calling_freebayes/main.nf
index 4708d06750..a078c157a7 100644
--- a/subworkflows/local/bam_variant_calling_freebayes/main.nf
+++ b/subworkflows/local/bam_variant_calling_freebayes/main.nf
@@ -20,7 +20,7 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     ch_intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = ch_cram.combine(ch_intervals)
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index 5da093ab4b..7e9b5c9469 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -52,31 +52,31 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     sentieon_dnascope_model           // channel: [mandatory] value channel with string
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
-    gvcf_sentieon_dnascope   = Channel.empty()
-    gvcf_sentieon_haplotyper = Channel.empty()
-
-    out_indexcov             = Channel.empty()
-    vcf_deepvariant          = Channel.empty()
-    vcf_freebayes            = Channel.empty()
-    vcf_haplotypecaller      = Channel.empty()
-    vcf_manta                = Channel.empty()
-    vcf_mpileup              = Channel.empty()
-    vcf_sentieon_dnascope    = Channel.empty()
-    vcf_sentieon_haplotyper  = Channel.empty()
-    vcf_strelka              = Channel.empty()
-    vcf_tiddit               = Channel.empty()
-    tbi_deepvariant          = Channel.empty()
-    tbi_freebayes            = Channel.empty()
-    tbi_haplotypecaller      = Channel.empty()
-    tbi_manta                = Channel.empty()
-    tbi_mpileup              = Channel.empty()
-    tbi_sentieon_dnascope    = Channel.empty()
-    tbi_sentieon_haplotyper  = Channel.empty()
-    tbi_strelka              = Channel.empty()
-    tbi_tiddit               = Channel.empty()
+    gvcf_sentieon_dnascope   = channel.empty()
+    gvcf_sentieon_haplotyper = channel.empty()
+
+    out_indexcov             = channel.empty()
+    vcf_deepvariant          = channel.empty()
+    vcf_freebayes            = channel.empty()
+    vcf_haplotypecaller      = channel.empty()
+    vcf_manta                = channel.empty()
+    vcf_mpileup              = channel.empty()
+    vcf_sentieon_dnascope    = channel.empty()
+    vcf_sentieon_haplotyper  = channel.empty()
+    vcf_strelka              = channel.empty()
+    vcf_tiddit               = channel.empty()
+    tbi_deepvariant          = channel.empty()
+    tbi_freebayes            = channel.empty()
+    tbi_haplotypecaller      = channel.empty()
+    tbi_manta                = channel.empty()
+    tbi_mpileup              = channel.empty()
+    tbi_sentieon_dnascope    = channel.empty()
+    tbi_sentieon_haplotyper  = channel.empty()
+    tbi_strelka              = channel.empty()
+    tbi_tiddit               = channel.empty()
 
     // BCFTOOLS MPILEUP
     if (tools && tools.split(',').contains('mpileup')) {
@@ -373,7 +373,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         versions = versions.mix(BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.versions)
     }
 
-    vcf_all = Channel.empty().mix(
+    vcf_all = channel.empty().mix(
         vcf_deepvariant,
         vcf_freebayes,
         vcf_sentieon_dnascope,
@@ -385,7 +385,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         vcf_tiddit
     )
 
-    tbi_all = Channel.empty().mix(
+    tbi_all = channel.empty().mix(
         tbi_deepvariant,
         tbi_freebayes,
         tbi_sentieon_dnascope,
diff --git a/subworkflows/local/bam_variant_calling_germline_manta/main.nf b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
index be68cc9114..1c086ec6ca 100644
--- a/subworkflows/local/bam_variant_calling_germline_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
@@ -15,7 +15,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi] or [ [], []] if no intervals; intervals file contains all intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ it ->
diff --git a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
index d2555144c9..c700d29cfd 100644
--- a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
+++ b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
@@ -19,10 +19,10 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
     intervals                    // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
-    vcf           = Channel.empty()
-    realigned_bam = Channel.empty()
+    vcf           = channel.empty()
+    realigned_bam = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -75,11 +75,11 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
     // Only when using intervals
     MERGE_HAPLOTYPECALLER(haplotypecaller_vcf.intervals.map{ meta, vcf_ -> [ groupKey(meta, meta.num_intervals), vcf_ ] }.groupTuple(), dict)
 
-    haplotypecaller_vcf = Channel.empty().mix(
+    haplotypecaller_vcf = channel.empty().mix(
             MERGE_HAPLOTYPECALLER.out.vcf,
             haplotypecaller_vcf.no_intervals)
 
-    haplotypecaller_tbi = Channel.empty().mix(
+    haplotypecaller_tbi = channel.empty().mix(
             MERGE_HAPLOTYPECALLER.out.tbi,
             haplotypecaller_tbi.no_intervals)
 
diff --git a/subworkflows/local/bam_variant_calling_indexcov/main.nf b/subworkflows/local/bam_variant_calling_indexcov/main.nf
index d1bc9f39a9..2f13b44f56 100644
--- a/subworkflows/local/bam_variant_calling_indexcov/main.nf
+++ b/subworkflows/local/bam_variant_calling_indexcov/main.nf
@@ -15,7 +15,7 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
     fasta_fai     // channel: [mandatory] [ meta, fasta_fai ]
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // generate a cleaner bam index without duplicate, supplementary, etc. (Small workload because the bam itself is not re-generated)
     reindex_ch = SAMTOOLS_REINDEX_BAM(
diff --git a/subworkflows/local/bam_variant_calling_mpileup/main.nf b/subworkflows/local/bam_variant_calling_mpileup/main.nf
index 5e21480c21..1c601a4993 100644
--- a/subworkflows/local/bam_variant_calling_mpileup/main.nf
+++ b/subworkflows/local/bam_variant_calling_mpileup/main.nf
@@ -18,7 +18,7 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram
diff --git a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
index fb409dd4d1..92570194dd 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
@@ -24,11 +24,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
     sentieon_dnascope_model           // channel
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
-    gvcf               = Channel.empty()
-    vcf                = Channel.empty()
-    genotype_intervals = Channel.empty()
+    gvcf               = channel.empty()
+    vcf                = channel.empty()
+    genotype_intervals = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals_for_sentieon = cram.combine(intervals)
@@ -114,11 +114,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
     // Only when using intervals
     MERGE_SENTIEON_DNASCOPE_VCFS(vcfs_for_merging, dict)
 
-    dnascope_vcf = Channel.empty().mix(
+    dnascope_vcf = channel.empty().mix(
         MERGE_SENTIEON_DNASCOPE_VCFS.out.vcf,
         dnascope_vcf_branch.no_intervals)
 
-    haplotyper_tbi = Channel.empty().mix(
+    haplotyper_tbi = channel.empty().mix(
         MERGE_SENTIEON_DNASCOPE_VCFS.out.tbi,
         dnascope_vcf_tbi_branch.no_intervals)
 
@@ -134,11 +134,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
 
     MERGE_SENTIEON_DNASCOPE_GVCFS(gvcfs_for_merging, dict)
 
-    gvcf = Channel.empty().mix(
+    gvcf = channel.empty().mix(
         MERGE_SENTIEON_DNASCOPE_GVCFS.out.vcf,
         haplotyper_gvcf_branch.no_intervals)
 
-    gvcf_tbi = Channel.empty().mix(
+    gvcf_tbi = channel.empty().mix(
         MERGE_SENTIEON_DNASCOPE_GVCFS.out.tbi,
         haplotyper_gvcf_tbi_branch.no_intervals)
 
diff --git a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
index cf30aaaf30..a2aba1ade3 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
@@ -22,11 +22,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
     sentieon_haplotyper_emit_mode
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
-    gvcf               = Channel.empty()
-    vcf                = Channel.empty()
-    genotype_intervals = Channel.empty()
+    gvcf               = channel.empty()
+    vcf                = channel.empty()
+    genotype_intervals = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals_for_sentieon = cram.combine(intervals)
@@ -111,11 +111,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
     // Only when using intervals
     MERGE_SENTIEON_HAPLOTYPER_VCFS(vcfs_for_merging, dict)
 
-    haplotyper_vcf = Channel.empty().mix(
+    haplotyper_vcf = channel.empty().mix(
         MERGE_SENTIEON_HAPLOTYPER_VCFS.out.vcf,
         haplotyper_vcf_branch.no_intervals)
 
-    haplotyper_tbi = Channel.empty().mix(
+    haplotyper_tbi = channel.empty().mix(
         MERGE_SENTIEON_HAPLOTYPER_VCFS.out.tbi,
         haplotyper_vcf_tbi_branch.no_intervals)
 
@@ -131,11 +131,11 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
 
     MERGE_SENTIEON_HAPLOTYPER_GVCFS(gvcfs_for_merging, dict)
 
-    gvcf = Channel.empty().mix(
+    gvcf = channel.empty().mix(
         MERGE_SENTIEON_HAPLOTYPER_GVCFS.out.vcf,
         haplotyper_gvcf_branch.no_intervals)
 
-    gvcf_tbi = Channel.empty().mix(
+    gvcf_tbi = channel.empty().mix(
         MERGE_SENTIEON_HAPLOTYPER_GVCFS.out.tbi,
         haplotyper_gvcf_tbi_branch.no_intervals)
 
diff --git a/subworkflows/local/bam_variant_calling_single_strelka/main.nf b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
index 2255a1bbda..6c469651e2 100644
--- a/subworkflows/local/bam_variant_calling_single_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
@@ -17,7 +17,7 @@ workflow BAM_VARIANT_CALLING_SINGLE_STRELKA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ] or [ [], [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -56,11 +56,11 @@ workflow BAM_VARIANT_CALLING_SINGLE_STRELKA {
 
     // Mix intervals and no_intervals channels together
     // Only strelka variant vcf should get annotated
-    vcf = Channel.empty().mix(MERGE_STRELKA.out.vcf, vcf_out.no_intervals)
+    vcf = channel.empty().mix(MERGE_STRELKA.out.vcf, vcf_out.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], vcf ] }
 
-    tbi = Channel.empty().mix(MERGE_STRELKA.out.tbi, tbi_out.no_intervals)
+    tbi = channel.empty().mix(MERGE_STRELKA.out.tbi, tbi_out.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], tbi ] }
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf b/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
index 22802cfb58..f771726eed 100644
--- a/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
@@ -19,7 +19,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ASCAT {
 
     main:
 
-    ch_versions = Channel.empty()
+    ch_versions = channel.empty()
 
     ASCAT(cram_pair, allele_files, loci_files, intervals_bed, fasta, gc_file, rt_file)
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
index 4d1edfaf59..e4ab083fca 100644
--- a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
@@ -14,7 +14,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi ] or [ [], [] ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ it ->
@@ -36,8 +36,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
 
     // Only diploid and somatic SV should get annotated
     // add variantcaller to meta map
-    vcf = Channel.empty().mix(diploid_sv_vcf, somatic_sv_vcf).map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
-    tbi = Channel.empty().mix(diploid_sv_vcf_tbi, somatic_sv_vcf_tbi).map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    vcf = channel.empty().mix(diploid_sv_vcf, somatic_sv_vcf).map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    tbi = channel.empty().mix(diploid_sv_vcf_tbi, somatic_sv_vcf_tbi).map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
     versions = versions.mix(MANTA_SOMATIC.out.versions)
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
index 95e7397f37..e8e9dea649 100644
--- a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
@@ -28,10 +28,10 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     joint_mutect2         // boolean: [mandatory] [default: false] run mutect2 in joint mode
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
-    // Handle Channel.value([]) input from prepare_genome by converting to proper empty channel
+    // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
     germline_resource_pileup = germline_resource.filter { it != [] }
     germline_resource_pileup_tbi = germline_resource_tbi.filter { it != [] }
 
@@ -100,22 +100,22 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     MERGEMUTECTSTATS(stats_to_merge)
 
     // Mix intervals and no_intervals channels together and remove no longer necessary field: normal_id, tumor_id, num_intervals
-    vcf = Channel.empty()
+    vcf = channel.empty()
         .mix(MERGE_MUTECT2.out.vcf, vcf_branch.no_intervals)
         .map { meta, vcf ->
             [joint_mutect2 ? meta - meta.subMap('normal_id', 'num_intervals') : meta - meta.subMap('num_intervals'), vcf]
         }
-    tbi = Channel.empty()
+    tbi = channel.empty()
         .mix(MERGE_MUTECT2.out.tbi, tbi_branch.no_intervals)
         .map { meta, tbi ->
             [joint_mutect2 ? meta - meta.subMap('normal_id', 'num_intervals') : meta - meta.subMap('num_intervals'), tbi]
         }
-    stats = Channel.empty()
+    stats = channel.empty()
         .mix(MERGEMUTECTSTATS.out.stats, stats_branch.no_intervals)
         .map { meta, stats ->
             [joint_mutect2 ? meta - meta.subMap('normal_id', 'num_intervals') : meta - meta.subMap('num_intervals'), stats]
         }
-    f1r2 = Channel.empty()
+    f1r2 = channel.empty()
         .mix(f1r2_to_merge, f1r2_branch.no_intervals)
         .map { meta, f1r2 ->
             [joint_mutect2 ? meta - meta.subMap('normal_id', 'num_intervals') : meta - meta.subMap('num_intervals'), f1r2]
@@ -162,8 +162,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
 
     // Do some channel magic to generate tumor-normal pairs again.
     // This is necessary because we generated one normal pileup summary for each patient but we need run calculate contamination for each tumor-normal pair.
-    pileup_table_tumor = Channel.empty().mix(GATHERPILEUPSUMMARIES_TUMOR.out.table, pileup_table_tumor_branch.no_intervals).map { meta, table -> [meta - meta.subMap('normal_id', 'tumor_id', 'num_intervals') + [id: meta.patient], meta.id, table] }
-    pileup_table_normal = Channel.empty().mix(GATHERPILEUPSUMMARIES_NORMAL.out.table, pileup_table_normal_branch.no_intervals).map { meta, table -> [meta - meta.subMap('normal_id', 'tumor_id', 'num_intervals') + [id: meta.patient], meta.id, table] }
+    pileup_table_tumor = channel.empty().mix(GATHERPILEUPSUMMARIES_TUMOR.out.table, pileup_table_tumor_branch.no_intervals).map { meta, table -> [meta - meta.subMap('normal_id', 'tumor_id', 'num_intervals') + [id: meta.patient], meta.id, table] }
+    pileup_table_normal = channel.empty().mix(GATHERPILEUPSUMMARIES_NORMAL.out.table, pileup_table_normal_branch.no_intervals).map { meta, table -> [meta - meta.subMap('normal_id', 'tumor_id', 'num_intervals') + [id: meta.patient], meta.id, table] }
 
     ch_calculatecontamination_in_tables = pileup_table_tumor
         .combine(
@@ -183,8 +183,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     CALCULATECONTAMINATION(ch_calculatecontamination_in_tables)
 
     // Initialize empty channel: Contamination calculation is run on pileup table, pileup is not run if germline resource is not provided
-    calculatecontamination_out_seg = Channel.empty()
-    calculatecontamination_out_cont = Channel.empty()
+    calculatecontamination_out_seg = channel.empty()
+    calculatecontamination_out_cont = channel.empty()
 
     if (joint_mutect2) {
         // Reduce the meta to only patient name
diff --git a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
index 7a983af722..eb960e5131 100644
--- a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
@@ -17,7 +17,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ] or [ [], [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -62,11 +62,11 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
     }
 
     // Mix intervals and no_intervals channels together
-    vcf = Channel.empty().mix(MERGE_STRELKA_INDELS.out.vcf, MERGE_STRELKA_SNVS.out.vcf, vcf_indels.no_intervals, vcf_snvs.no_intervals)
+    vcf = channel.empty().mix(MERGE_STRELKA_INDELS.out.vcf, MERGE_STRELKA_SNVS.out.vcf, vcf_indels.no_intervals, vcf_snvs.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], vcf ] }
 
-    tbi = Channel.empty().mix(MERGE_STRELKA_INDELS.out.tbi, MERGE_STRELKA_SNVS.out.tbi, tbi_indels.no_intervals, tbi_snvs.no_intervals)
+    tbi = channel.empty().mix(MERGE_STRELKA_INDELS.out.tbi, MERGE_STRELKA_SNVS.out.tbi, tbi_indels.no_intervals, tbi_snvs.no_intervals)
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], tbi ] }
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf b/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
index f768c42791..30eed94927 100644
--- a/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
@@ -17,7 +17,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TIDDIT {
 
     main:
 
-    versions = Channel.empty()
+    versions = channel.empty()
 
     TIDDIT_NORMAL(cram_normal, fasta, bwa)
     TIDDIT_TUMOR(cram_tumor, fasta, bwa)
diff --git a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
index 7a306fffa3..8f0e083a59 100644
--- a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
@@ -19,7 +19,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TNSCOPE {
     intervals                 // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine input and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
@@ -57,11 +57,11 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TNSCOPE {
 
     // Mix intervals and no_intervals channels together
     // Remove unnecessary metadata and add variantcaller
-    vcf   = Channel.empty()
+    vcf   = channel.empty()
         .mix(MERGE_TNSCOPE.out.vcf, vcf_branch.no_intervals)
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'sentieon_tnscope' ], vcf ] }
 
-    index = Channel.empty()
+    index = channel.empty()
         .mix(MERGE_TNSCOPE.out.tbi, tbi_branch.no_intervals)
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'sentieon_tnscope' ], tbi ] }
 
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 0613a74b8b..1a478957b1 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -45,26 +45,26 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
     // Channels are often remapped to match module/subworkflow
 
     // Gather all versions
-    versions = Channel.empty()
+    versions = channel.empty()
 
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
-    out_msisensor2 = Channel.empty()
-    vcf_freebayes  = Channel.empty()
-    vcf_lofreq     = Channel.empty()
-    vcf_manta      = Channel.empty()
-    vcf_mpileup    = Channel.empty()
-    vcf_mutect2    = Channel.empty()
-    vcf_tiddit     = Channel.empty()
-    vcf_tnscope    = Channel.empty()
+    out_msisensor2 = channel.empty()
+    vcf_freebayes  = channel.empty()
+    vcf_lofreq     = channel.empty()
+    vcf_manta      = channel.empty()
+    vcf_mpileup    = channel.empty()
+    vcf_mutect2    = channel.empty()
+    vcf_tiddit     = channel.empty()
+    vcf_tnscope    = channel.empty()
 
     // Initialize empty TBI channels
-    tbi_freebayes  = Channel.empty()
-    tbi_lofreq     = Channel.empty()
-    tbi_manta      = Channel.empty()
-    tbi_mpileup    = Channel.empty()
-    tbi_mutect2    = Channel.empty()
-    tbi_tiddit     = Channel.empty()
-    tbi_tnscope    = Channel.empty()
+    tbi_freebayes  = channel.empty()
+    tbi_lofreq     = channel.empty()
+    tbi_manta      = channel.empty()
+    tbi_mpileup    = channel.empty()
+    tbi_mutect2    = channel.empty()
+    tbi_tiddit     = channel.empty()
+    tbi_tnscope    = channel.empty()
 
     // MPILEUP
     if (tools && tools.split(',').contains('mpileup') || tools.split(',').contains('controlfreec')) {
@@ -218,7 +218,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
         versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE.out.versions)
     }
 
-    vcf_all = Channel.empty()
+    vcf_all = channel.empty()
         .mix(
             vcf_freebayes,
             vcf_lofreq,
@@ -229,7 +229,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
             vcf_tnscope,
         )
 
-    tbi_all = Channel.empty()
+    tbi_all = channel.empty()
         .mix(
             tbi_freebayes,
             tbi_lofreq,
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
index 681232a39b..702d6361c8 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
@@ -10,7 +10,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ {
     dict      // channel: /path/to/reference/fasta/dictionary
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
@@ -40,8 +40,8 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ {
 
     // Mix intervals and no_intervals channels together
     // Remove unnecessary metadata
-    vcf = Channel.empty().mix(MERGE_LOFREQ.out.vcf, vcf_branch.no_intervals).map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], vcf ] }
-    tbi = Channel.empty().mix(MERGE_LOFREQ.out.tbi, tbi_branch.no_intervals).map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], tbi ] }
+    vcf = channel.empty().mix(MERGE_LOFREQ.out.vcf, vcf_branch.no_intervals).map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], vcf ] }
+    tbi = channel.empty().mix(MERGE_LOFREQ.out.tbi, tbi_branch.no_intervals).map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], tbi ] }
 
     versions = versions.mix(LOFREQ.out.versions)
 
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
index b451c3962d..9fc89e931c 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
@@ -15,7 +15,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi ] or [ [], [] ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ it ->
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
index f3228c9a86..bfd4c278ac 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
@@ -27,10 +27,10 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     joint_mutect2         // boolean: [mandatory] [default: false] run mutect2 in joint mode
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
-    // Handle Channel.value([]) input from prepare_genome by converting to proper empty channel
+    // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
     germline_resource_pileup = germline_resource.filter { it != [] }
     germline_resource_pileup_tbi = germline_resource_tbi.filter { it != [] }
 
@@ -91,10 +91,10 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
 
     // Mix intervals and no_intervals channels together
     // Remove unnecessary metadata
-    vcf = Channel.empty().mix(MERGE_MUTECT2.out.vcf, vcf_branch.no_intervals).map { meta, vcf -> [meta - meta.subMap('num_intervals'), vcf] }
-    tbi = Channel.empty().mix(MERGE_MUTECT2.out.tbi, tbi_branch.no_intervals).map { meta, tbi -> [meta - meta.subMap('num_intervals'), tbi] }
-    stats = Channel.empty().mix(MERGEMUTECTSTATS.out.stats, stats_branch.no_intervals).map { meta, stats -> [meta - meta.subMap('num_intervals'), stats] }
-    f1r2 = Channel.empty().mix(f1r2_to_merge, f1r2_branch.no_intervals).map { meta, f1r2 -> [meta - meta.subMap('num_intervals'), f1r2] }
+    vcf = channel.empty().mix(MERGE_MUTECT2.out.vcf, vcf_branch.no_intervals).map { meta, vcf -> [meta - meta.subMap('num_intervals'), vcf] }
+    tbi = channel.empty().mix(MERGE_MUTECT2.out.tbi, tbi_branch.no_intervals).map { meta, tbi -> [meta - meta.subMap('num_intervals'), tbi] }
+    stats = channel.empty().mix(MERGEMUTECTSTATS.out.stats, stats_branch.no_intervals).map { meta, stats -> [meta - meta.subMap('num_intervals'), stats] }
+    f1r2 = channel.empty().mix(f1r2_to_merge, f1r2_branch.no_intervals).map { meta, f1r2 -> [meta - meta.subMap('num_intervals'), f1r2] }
 
     // Generate artifactpriors using learnreadorientationmodel on the f1r2 output of mutect2
     LEARNREADORIENTATIONMODEL(f1r2)
@@ -116,14 +116,14 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     GATHERPILEUPSUMMARIES(pileup_table_to_merge, dict.map { _meta, dict_ -> [dict_] })
 
     // Mix intervals and no_intervals channels together
-    pileup_table = Channel.empty().mix(GATHERPILEUPSUMMARIES.out.table, pileup_table_branch.no_intervals).map { meta, table -> [meta - meta.subMap('num_intervals') + [id: meta.sample], table] }
+    pileup_table = channel.empty().mix(GATHERPILEUPSUMMARIES.out.table, pileup_table_branch.no_intervals).map { meta, table -> [meta - meta.subMap('num_intervals') + [id: meta.sample], table] }
 
     // Contamination and segmentation tables created using calculatecontamination on the pileup summary table
     CALCULATECONTAMINATION(pileup_table.map { meta, table -> [meta, table, []] })
 
     // Initialize empty channel: Contamination calculation is run on pileup table, pileup is not run if germline resource is not provided
-    calculatecontamination_out_seg = Channel.empty()
-    calculatecontamination_out_cont = Channel.empty()
+    calculatecontamination_out_seg = channel.empty()
+    calculatecontamination_out_cont = channel.empty()
 
     if (joint_mutect2) {
         // Group tables by samples
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
index 4f86b0d2c4..91ec26a72b 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
@@ -19,7 +19,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE {
     intervals                 // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Combine input and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
@@ -57,11 +57,11 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE {
 
     // Mix intervals and no_intervals channels together
     // Remove unnecessary metadata and add variantcaller
-    vcf   = Channel.empty()
+    vcf   = channel.empty()
         .mix(MERGE_TNSCOPE.out.vcf, vcf_branch.no_intervals)
         .map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'sentieon_tnscope' ], vcf ] }
 
-    index = Channel.empty()
+    index = channel.empty()
         .mix(MERGE_TNSCOPE.out.tbi, tbi_branch.no_intervals)
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'sentieon_tnscope' ], tbi ] }
 
diff --git a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
index e53e69360e..02985a7939 100644
--- a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
+++ b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
@@ -14,8 +14,8 @@ workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
     intervals
 
     main:
-    versions = Channel.empty()
-    reports = Channel.empty()
+    versions = channel.empty()
+    reports = channel.empty()
 
     // Reports run on cram
     SAMTOOLS_STATS(cram, fasta)
diff --git a/subworkflows/local/post_variantcalling/tests/main.nf.test b/subworkflows/local/post_variantcalling/tests/main.nf.test
index b19b1117e9..934420065d 100644
--- a/subworkflows/local/post_variantcalling/tests/main.nf.test
+++ b/subworkflows/local/post_variantcalling/tests/main.nf.test
@@ -19,15 +19,15 @@ nextflow_workflow {
                 cram_germline                     = [[:],[]]
                 germline_vcfs                     = channel.of([[id: 'test_normal'],
                                                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)])
-                germline_tbis                     = Channel.empty()
+                germline_tbis                     = channel.empty()
                 cram_tumor_only                   = [[:],[]]
                 tumor_only_vcfs                   = channel.of([[id: 'test_tumor'],
                                                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)])
-                tumor_only_tbis                   = Channel.empty()
+                tumor_only_tbis                   = channel.empty()
                 cram_somatic                      = [[:],[]]
                 somatic_vcfs                      = channel.of([[id: 'test_somatic'],
                                                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)])
-                somatic_tbis                      = Channel.empty()
+                somatic_tbis                      = channel.empty()
                 fasta                             = channel.of([[id: 'fasta'],
                                                         file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
                 fai                               = [[:],[]]
@@ -84,20 +84,20 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = ''
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', num: '1'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)],
                     [[id: 'test_sample', num: '2'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = true
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
@@ -151,20 +151,20 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = ''
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', num: '1'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)],
                     [[id: 'test_sample', num: '2'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = true
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
@@ -218,19 +218,19 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'strelka'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
@@ -283,19 +283,19 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'strelka'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
@@ -351,19 +351,19 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'haplotypecaller,strelka'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = true
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
@@ -421,29 +421,29 @@ nextflow_workflow {
                 """
                 // Input setup with real test data
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.of(
+                cram_germline                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists:true), file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists:true)]
                 )
-                germline_vcfs                     = Channel.of(
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = params.varlociraptor_chunk_size
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -492,29 +492,29 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.of(
+                cram_germline                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists:true), file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists:true)]
                 )
-                germline_vcfs                     = Channel.of(
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = 2
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -564,29 +564,29 @@ nextflow_workflow {
                 """
                 // Input setup with real test data
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.empty()
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.empty()
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XY', status: 1, contamination: 0.0], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists:true), file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists:true)]
                 )
-                tumor_only_vcfs                   = Channel.of(
+                tumor_only_vcfs                   = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XY', status: 1, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = params.varlociraptor_chunk_size
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -634,29 +634,29 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.empty()
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.empty()
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XY', status: 1, contamination: 0.0], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists:true), file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists:true)]
                 )
-                tumor_only_vcfs                   = Channel.of(
+                tumor_only_vcfs                   = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XY', status: 1, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = 2
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -706,15 +706,15 @@ nextflow_workflow {
                 """
                 // Input setup with real test data
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'normal_sample', patient: 'test', sample: 'normal', sex: 'XX', status: 0, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.of(
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.of(
 
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XX', status: 1, normal_id: 'normal_sample', contamination: 0.0],
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram', checkIfExists:true),
@@ -722,21 +722,21 @@ nextflow_workflow {
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram.crai', checkIfExists: true)]
                 )
-                somatic_vcfs                      = Channel.of(
+                somatic_vcfs                      = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XX', status: 1, normal_id: 'normal_sample', variantcaller: 'strelka'],
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_filtered_mutect2_calls.vcf.gz', checkIfExists: true)]
                 )
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true)])
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = params.varlociraptor_chunk_size
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -786,35 +786,35 @@ nextflow_workflow {
                 """
                 // Input setup
                 tools                             = 'varlociraptor'
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'normal_sample', patient: 'test', sample: 'normal', sex: 'XX', status: 0, variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.empty()
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.of(
+                germline_tbis                     = channel.empty()
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XX', status: 1, normal_id: 'normal_sample', contamination: 0.0],
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam', checkIfExists:true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai', checkIfExists:true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam', checkIfExists: true),
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam.bai', checkIfExists: true)]
                 )
-                somatic_vcfs                      = Channel.of(
+                somatic_vcfs                      = channel.of(
                     [[id: 'tumor_sample', patient: 'test', sample: 'tumor', sex: 'XX', status: 1, normal_id: 'normal_sample', variantcaller: 'strelka'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_filtered_mutect2_calls.vcf.gz', checkIfExists: true)]
                 )
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)])
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = false
                 normalize_vcfs                    = false
                 varlociraptor_chunk_size          = 2
-                varlociraptor_scenario_germline   = Channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
-                varlociraptor_scenario_somatic    = Channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
-                varlociraptor_scenario_tumor_only = Channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
+                varlociraptor_scenario_germline   = channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect()
+                varlociraptor_scenario_somatic    = channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect()
+                varlociraptor_scenario_tumor_only = channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect()
 
                 input[0]  = tools
                 input[1]  = cram_germline
@@ -859,27 +859,27 @@ nextflow_workflow {
                 """
                 // Input setup for consensus calling - requires multiple VCFs with different variantcallers
                 tools                             = ''
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.of(
+                germline_tbis                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)]
                 )
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = true
@@ -932,27 +932,27 @@ nextflow_workflow {
                 """
                 // Input setup for full post-processing pipeline
                 tools                             = ''
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'haplotypecaller'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.of(
+                germline_tbis                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'haplotypecaller'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)]
                 )
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = true
                 snv_consensus_calling             = true
@@ -1007,27 +1007,27 @@ nextflow_workflow {
                 """
                 // Input setup for consensus stub test
                 tools                             = ''
-                cram_germline                     = Channel.empty()
-                germline_vcfs                     = Channel.of(
+                cram_germline                     = channel.empty()
+                germline_vcfs                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true)]
                 )
-                germline_tbis                     = Channel.of(
+                germline_tbis                     = channel.of(
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'freebayes'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)],
                     [[id: 'test_sample', patient: 'test', sample: 'test', sex: 'XX', status: 0, variantcaller: 'strelka'],
                         file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true)]
                 )
-                cram_tumor_only                   = Channel.empty()
-                tumor_only_vcfs                   = Channel.empty()
-                tumor_only_tbis                   = Channel.empty()
-                cram_somatic                      = Channel.empty()
-                somatic_vcfs                      = Channel.empty()
-                somatic_tbis                      = Channel.empty()
-                fasta                             = Channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
-                fai                               = Channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
+                cram_tumor_only                   = channel.empty()
+                tumor_only_vcfs                   = channel.empty()
+                tumor_only_tbis                   = channel.empty()
+                cram_somatic                      = channel.empty()
+                somatic_vcfs                      = channel.empty()
+                somatic_tbis                      = channel.empty()
+                fasta                             = channel.of([[id: 'fasta'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])
+                fai                               = channel.of([[id: 'fai'], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true)])
                 concatenate_vcfs                  = false
                 filter_vcfs                       = false
                 snv_consensus_calling             = true
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 5460704157..a78e0706da 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -54,10 +54,10 @@ workflow PREPARE_GENOME {
     vep_include_fasta           // params.vep_include_fasta
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // TODO: EXTRACT FASTA FILE?
-    fasta = fasta_in ? Channel.fromPath(fasta_in).map { fasta -> [[id: fasta.baseName], fasta] }.collect() : Channel.empty()
+    fasta = fasta_in ? channel.fromPath(fasta_in).map { fasta -> [[id: fasta.baseName], fasta] }.collect() : channel.empty()
     vep_fasta = vep_include_fasta ? fasta : [[id: 'null'], []]
 
     if (step == 'mapping') {
@@ -67,7 +67,7 @@ workflow PREPARE_GENOME {
             versions = versions.mix(BWAMEM1_INDEX.out.versions)
         }
         else if (aligner == "bwa-mem" || aligner == "sentieon-bwamem" || aligner == "parabricks") {
-            index_alignment = Channel.fromPath(bwa_in).map { index -> [[id: 'bwa'], index] }.collect()
+            index_alignment = channel.fromPath(bwa_in).map { index -> [[id: 'bwa'], index] }.collect()
         }
         else if (!bwamem2_in && aligner == 'bwa-mem2') {
             BWAMEM2_INDEX(fasta)
@@ -75,7 +75,7 @@ workflow PREPARE_GENOME {
             versions = versions.mix(BWAMEM2_INDEX.out.versions)
         }
         else if (aligner == 'bwa-mem2') {
-            index_alignment = Channel.fromPath(bwamem2_in).map { index -> [[id: 'bwamem2'], index] }.collect()
+            index_alignment = channel.fromPath(bwamem2_in).map { index -> [[id: 'bwamem2'], index] }.collect()
         }
         else if (!dragmap_in && aligner == 'dragmap') {
             DRAGMAP_HASHTABLE(fasta)
@@ -83,11 +83,11 @@ workflow PREPARE_GENOME {
             versions = versions.mix(DRAGMAP_HASHTABLE.out.versions)
         }
         else if (aligner == 'dragmap') {
-            index_alignment = Channel.fromPath(dragmap_in).map { index -> [[id: 'dragmap'], index] }.collect()
+            index_alignment = channel.fromPath(dragmap_in).map { index -> [[id: 'dragmap'], index] }.collect()
         }
     }
     else {
-        index_alignment = Channel.empty()
+        index_alignment = channel.empty()
     }
 
     if (!dict_in && step != "annotate") {
@@ -96,10 +96,10 @@ workflow PREPARE_GENOME {
         versions = versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
     }
     else if (dict_in) {
-        dict = Channel.fromPath(dict_in).map { it -> [[id: 'dict'], it] }.collect()
+        dict = channel.fromPath(dict_in).map { it -> [[id: 'dict'], it] }.collect()
     }
     else {
-        dict = Channel.empty()
+        dict = channel.empty()
     }
 
     if (!fasta_fai_in && step != "annotate") {
@@ -108,14 +108,14 @@ workflow PREPARE_GENOME {
         versions = versions.mix(SAMTOOLS_FAIDX.out.versions)
     }
     else if (fasta_fai_in) {
-        fasta_fai = Channel.fromPath(fasta_fai_in).map { it -> [[id: 'fai'], it] }.collect()
+        fasta_fai = channel.fromPath(fasta_fai_in).map { it -> [[id: 'fai'], it] }.collect()
     }
     else {
-        fasta_fai = Channel.empty()
+        fasta_fai = channel.empty()
     }
 
     // Prepare genome for BBSplit contamination filtering
-    bbsplit_index = Channel.empty()
+    bbsplit_index = channel.empty()
     if (tools && tools.split(',').contains('bbsplit')) {
         if (bbsplit_index_in) {
             // Use user-provided bbsplit index
@@ -124,12 +124,12 @@ workflow PREPARE_GENOME {
                 versions = versions.mix(UNTAR_BBSPLIT_INDEX.out.versions)
             }
             else {
-                bbsplit_index = Channel.value(file(bbsplit_index_in, checkIfExists: true))
+                bbsplit_index = channel.value(file(bbsplit_index_in, checkIfExists: true))
             }
         }
         else if (bbsplit_fasta_list_in) {
             // Build it from scratch if we have FASTA
-            Channel.from(file(bbsplit_fasta_list_in, checkIfExists: true))
+            channel.from(file(bbsplit_fasta_list_in, checkIfExists: true))
                 .splitCsv(header: false, sep: ',')
                 .flatMap { id, fafile -> [['id', id], ['fasta', file(fafile, checkIfExists: true)]] }
                 .groupTuple()
@@ -147,8 +147,8 @@ workflow PREPARE_GENOME {
         }
     }
 
-    bcftools_annotations = bcftools_annotations_in ? Channel.fromPath(bcftools_annotations_in).collect() : Channel.value([])
-    bcftools_annotations_tbi = bcftools_annotations_tbi_in ? Channel.fromPath(bcftools_annotations_tbi_in).collect() : Channel.value([])
+    bcftools_annotations = bcftools_annotations_in ? channel.fromPath(bcftools_annotations_in).collect() : channel.value([])
+    bcftools_annotations_tbi = bcftools_annotations_tbi_in ? channel.fromPath(bcftools_annotations_tbi_in).collect() : channel.value([])
 
     if (!bcftools_annotations_tbi_in && bcftools_annotations_in) {
         TABIX_BCFTOOLS_ANNOTATIONS(bcftools_annotations.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -156,8 +156,8 @@ workflow PREPARE_GENOME {
 
     }
 
-    dbsnp = dbsnp_in ? Channel.fromPath(dbsnp_in).collect() : Channel.value([])
-    dbsnp_tbi = dbsnp_tbi_in ? Channel.fromPath(dbsnp_tbi_in).collect() : Channel.value([])
+    dbsnp = dbsnp_in ? channel.fromPath(dbsnp_in).collect() : channel.value([])
+    dbsnp_tbi = dbsnp_tbi_in ? channel.fromPath(dbsnp_tbi_in).collect() : channel.value([])
 
     if (!dbsnp_tbi_in && dbsnp_in && ((step == "mapping" || step == "markduplicates" || step == "prepare_recalibration") || (tools.split(',').contains('controlfreec') || tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope') || tools.split(',').contains('muse') || tools.split(',').contains('mutect2')))) {
         TABIX_DBSNP(dbsnp.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -165,8 +165,8 @@ workflow PREPARE_GENOME {
         versions = versions.mix(TABIX_DBSNP.out.versions)
     }
 
-    germline_resource = germline_resource_in ? Channel.fromPath(germline_resource_in).collect() : Channel.value([])
-    germline_resource_tbi = germline_resource_tbi_in ? Channel.fromPath(germline_resource_tbi_in).collect() : Channel.value([])
+    germline_resource = germline_resource_in ? channel.fromPath(germline_resource_in).collect() : channel.value([])
+    germline_resource_tbi = germline_resource_tbi_in ? channel.fromPath(germline_resource_tbi_in).collect() : channel.value([])
 
     if (!germline_resource_tbi_in && germline_resource_in && (tools.split(',').contains('mutect2') || tools.split(',').contains('sentieon_tnscope'))) {
         TABIX_GERMLINE_RESOURCE(germline_resource.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -174,8 +174,8 @@ workflow PREPARE_GENOME {
         versions = versions.mix(TABIX_GERMLINE_RESOURCE.out.versions)
     }
 
-    known_indels = known_indels_in ? Channel.fromPath(known_indels_in).collect() : Channel.value([])
-    known_indels_tbi = known_indels_tbi_in ? Channel.fromPath(known_indels_tbi_in).collect() : Channel.value([])
+    known_indels = known_indels_in ? channel.fromPath(known_indels_in).collect() : channel.value([])
+    known_indels_tbi = known_indels_tbi_in ? channel.fromPath(known_indels_tbi_in).collect() : channel.value([])
 
     if (!known_indels_tbi_in && known_indels_in && (step == 'mapping' || step == "markduplicates" || step == 'prepare_recalibration' || (tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope')))) {
         TABIX_KNOWN_INDELS(known_indels.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -183,8 +183,8 @@ workflow PREPARE_GENOME {
         versions = versions.mix(TABIX_KNOWN_INDELS.out.versions)
     }
 
-    known_snps = known_snps_in ? Channel.fromPath(known_snps_in).collect() : Channel.value([])
-    known_snps_tbi = known_snps_tbi_in ? Channel.fromPath(known_snps_tbi_in).collect() : Channel.value([])
+    known_snps = known_snps_in ? channel.fromPath(known_snps_in).collect() : channel.value([])
+    known_snps_tbi = known_snps_tbi_in ? channel.fromPath(known_snps_tbi_in).collect() : channel.value([])
 
     if (!known_snps_tbi_in && known_snps_in && (step == 'mapping' || step == "markduplicates" || step == 'prepare_recalibration' || (tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper')))) {
         TABIX_KNOWN_SNPS(known_snps.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -192,8 +192,8 @@ workflow PREPARE_GENOME {
         versions = versions.mix(TABIX_KNOWN_SNPS.out.versions)
     }
 
-    pon = pon_in ? Channel.fromPath(pon_in).collect() : Channel.value([])
-    pon_tbi = pon_tbi_in ? Channel.fromPath(pon_tbi_in).collect() : Channel.value([])
+    pon = pon_in ? channel.fromPath(pon_in).collect() : channel.value([])
+    pon_tbi = pon_tbi_in ? channel.fromPath(pon_tbi_in).collect() : channel.value([])
 
     if (!pon_tbi_in && pon_in && tools.split(',').contains('mutect2')) {
         TABIX_PON(pon.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
@@ -210,19 +210,19 @@ workflow PREPARE_GENOME {
 
     // MSI
     if (msisensor2_models_in && msisensor2_models_in.endsWith(".tar.gz") && tools.split(',').contains('msisensor2')) {
-        UNTAR_MSISENSOR2_MODELS(Channel.fromPath(file(msisensor2_models_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNTAR_MSISENSOR2_MODELS(channel.fromPath(file(msisensor2_models_in)).map { archive -> [[id: archive.baseName], archive] })
         msisensor2_models = UNTAR_MSISENSOR2_MODELS.out.untar.collect()
         versions = versions.mix(UNTAR_MSISENSOR2_MODELS.out.versions)
     }
     else if (msisensor2_models_in && tools.split(',').contains('msisensor2')) {
-        msisensor2_models = Channel.fromPath(msisensor2_models_in).map { model -> [[id:model.baseName], model] }.collect()
+        msisensor2_models = channel.fromPath(msisensor2_models_in).map { model -> [[id:model.baseName], model] }.collect()
     }
     else {
-        msisensor2_models = Channel.value([])
+        msisensor2_models = channel.value([])
     }
 
     if (msisensorpro_scan_in) {
-        msisensorpro_scan = Channel.fromPath(msisensorpro_scan_in)
+        msisensorpro_scan = channel.fromPath(msisensorpro_scan_in)
     }
     else if (tools.split(',').contains('msisensorpro')) {
         MSISENSORPRO_SCAN(fasta)
@@ -230,68 +230,68 @@ workflow PREPARE_GENOME {
         versions = versions.mix(MSISENSORPRO_SCAN.out.versions)
     }
     else {
-        msisensorpro_scan = Channel.value([])
+        msisensorpro_scan = channel.value([])
     }
 
     // prepare ascat and controlfreec reference files
     if (!ascat_alleles_in) {
-        ascat_alleles = Channel.empty()
+        ascat_alleles = channel.empty()
     }
     else if (ascat_alleles_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
-        UNZIP_ALLELES(Channel.fromPath(file(ascat_alleles_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNZIP_ALLELES(channel.fromPath(file(ascat_alleles_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_alleles = UNZIP_ALLELES.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
         versions = versions.mix(UNZIP_ALLELES.out.versions)
     }
     else {
-        ascat_alleles = Channel.fromPath(ascat_alleles_in).collect()
+        ascat_alleles = channel.fromPath(ascat_alleles_in).collect()
     }
 
     if (!ascat_loci_in) {
-        ascat_loci = Channel.empty()
+        ascat_loci = channel.empty()
     }
     else if (ascat_loci_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
-        UNZIP_LOCI(Channel.fromPath(file(ascat_loci_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNZIP_LOCI(channel.fromPath(file(ascat_loci_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci = UNZIP_LOCI.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
         versions = versions.mix(UNZIP_LOCI.out.versions)
     }
     else {
-        ascat_loci = Channel.fromPath(ascat_loci_in).collect()
+        ascat_loci = channel.fromPath(ascat_loci_in).collect()
     }
 
     if (!ascat_loci_gc_in) {
-        ascat_loci_gc = Channel.value([])
+        ascat_loci_gc = channel.value([])
     }
     else if (ascat_loci_gc_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
-        UNZIP_GC(Channel.fromPath(file(ascat_loci_gc_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNZIP_GC(channel.fromPath(file(ascat_loci_gc_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci_gc = UNZIP_GC.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
         versions = versions.mix(UNZIP_GC.out.versions)
     }
     else {
-        ascat_loci_gc = Channel.fromPath(ascat_loci_gc_in).collect()
+        ascat_loci_gc = channel.fromPath(ascat_loci_gc_in).collect()
     }
 
     if (!ascat_loci_rt_in) {
-        ascat_loci_rt = Channel.value([])
+        ascat_loci_rt = channel.value([])
     }
     else if (ascat_loci_rt_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
-        UNZIP_RT(Channel.fromPath(file(ascat_loci_rt_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNZIP_RT(channel.fromPath(file(ascat_loci_rt_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci_rt = UNZIP_RT.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
         versions = versions.mix(UNZIP_RT.out.versions)
     }
     else {
-        ascat_loci_rt = Channel.fromPath(ascat_loci_rt_in).collect()
+        ascat_loci_rt = channel.fromPath(ascat_loci_rt_in).collect()
     }
 
     if (!chr_dir_in) {
-        chr_dir = Channel.value([])
+        chr_dir = channel.value([])
     }
     else if (chr_dir_in.endsWith(".tar.gz") && tools.split(',').contains('controlfreec')) {
-        UNTAR_CHR_DIR(Channel.fromPath(file(chr_dir_in)).map { archive -> [[id: archive.baseName], archive] })
+        UNTAR_CHR_DIR(channel.fromPath(file(chr_dir_in)).map { archive -> [[id: archive.baseName], archive] })
         chr_dir = UNTAR_CHR_DIR.out.untar.map { _meta, extracted_archive -> extracted_archive }.collect()
         versions = versions.mix(UNTAR_CHR_DIR.out.versions)
     }
     else {
-        chr_dir = Channel.fromPath(chr_dir_in).collect()
+        chr_dir = channel.fromPath(chr_dir_in).collect()
     }
 
     emit:
diff --git a/subworkflows/local/prepare_genome/tests/bbsplit.nf.test b/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
index ca96469853..026ed1dd9c 100644
--- a/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
+++ b/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
@@ -25,27 +25,27 @@ nextflow_workflow {
                 input[3]  = null                                                                                                                                       // ascat_loci_rt
                 input[4]  = "${projectDir}/tests/csv/bbsplit_fasta_list.csv"                                                                                           // bbsplit_fasta_list
                 input[5]  = null                                                                                                                                       // bbsplit_index
-                input[6]  = Channel.empty()                                                                                                                            // bcftools_annotations
-                input[7]  = Channel.empty()                                                                                                                            // bcftools_annotations_tbi
+                input[6]  = channel.empty()                                                                                                                            // bcftools_annotations
+                input[7]  = channel.empty()                                                                                                                            // bcftools_annotations_tbi
                 input[8]  = null                                                                                                                                       // bwa
                 input[9]  = null                                                                                                                                       // bwamem2
                 input[10] = null                                                                                                                                       // chr_dir
-                input[11] = Channel.empty()                                                                                                                            // dbsnp
-                input[12] = Channel.empty()                                                                                                                            // dbsnp_tbi
+                input[11] = channel.empty()                                                                                                                            // dbsnp
+                input[12] = channel.empty()                                                                                                                            // dbsnp_tbi
                 input[13] = null                                                                                                                                       // dict
                 input[14] = null                                                                                                                                       // dragmap
-                input[15] = Channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
+                input[15] = channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
                 input[16] = null                                                                                                                                       // fasta_fai
-                input[17] = Channel.empty()                                                                                                                            // germline_resource
-                input[18] = Channel.empty()                                                                                                                            // germline_resource_tbi
-                input[19] = Channel.empty()                                                                                                                            // known_indels
-                input[20] = Channel.empty()                                                                                                                            // known_indels_tbi
-                input[21] = Channel.empty()                                                                                                                            // known_snps
-                input[22] = Channel.empty()                                                                                                                            // known_snps_tbi
+                input[17] = channel.empty()                                                                                                                            // germline_resource
+                input[18] = channel.empty()                                                                                                                            // germline_resource_tbi
+                input[19] = channel.empty()                                                                                                                            // known_indels
+                input[20] = channel.empty()                                                                                                                            // known_indels_tbi
+                input[21] = channel.empty()                                                                                                                            // known_snps
+                input[22] = channel.empty()                                                                                                                            // known_snps_tbi
                 input[23] = null                                                                                                                                       // msisensor2_models
                 input[24] = null                                                                                                                                       // msisensorpro_scan
-                input[25] = Channel.empty()                                                                                                                            // pon
-                input[26] = Channel.empty()                                                                                                                            // pon_tbi
+                input[25] = channel.empty()                                                                                                                            // pon
+                input[26] = channel.empty()                                                                                                                            // pon_tbi
                 input[27] = 'bwa-mem'                                                                                                                                  // aligner
                 input[28] = 'mapping'                                                                                                                                  // step
                 input[29] = "bbsplit"                                                                                                                                  // tools - enable bbsplit
@@ -81,27 +81,27 @@ nextflow_workflow {
                 input[3]  = null                                                                                                                                       // ascat_loci_rt
                 input[4]  = null                                                                                                                                       // bbsplit_fasta_list
                 input[5]  = null                                                                                                                                       // bbsplit_index
-                input[6]  = Channel.empty()                                                                                                                            // bcftools_annotations
-                input[7]  = Channel.empty()                                                                                                                            // bcftools_annotations_tbi
+                input[6]  = channel.empty()                                                                                                                            // bcftools_annotations
+                input[7]  = channel.empty()                                                                                                                            // bcftools_annotations_tbi
                 input[8]  = null                                                                                                                                       // bwa
                 input[9]  = null                                                                                                                                       // bwamem2
                 input[10] = null                                                                                                                                       // chr_dir
-                input[11] = Channel.empty()                                                                                                                            // dbsnp
-                input[12] = Channel.empty()                                                                                                                            // dbsnp_tbi
+                input[11] = channel.empty()                                                                                                                            // dbsnp
+                input[12] = channel.empty()                                                                                                                            // dbsnp_tbi
                 input[13] = null                                                                                                                                       // dict
                 input[14] = null                                                                                                                                       // dragmap
-                input[15] = Channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
+                input[15] = channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
                 input[16] = null                                                                                                                                       // fasta_fai
-                input[17] = Channel.empty()                                                                                                                            // germline_resource
-                input[18] = Channel.empty()                                                                                                                            // germline_resource_tbi
-                input[19] = Channel.empty()                                                                                                                            // known_indels
-                input[20] = Channel.empty()                                                                                                                            // known_indels_tbi
-                input[21] = Channel.empty()                                                                                                                            // known_snps
-                input[22] = Channel.empty()                                                                                                                            // known_snps_tbi
+                input[17] = channel.empty()                                                                                                                            // germline_resource
+                input[18] = channel.empty()                                                                                                                            // germline_resource_tbi
+                input[19] = channel.empty()                                                                                                                            // known_indels
+                input[20] = channel.empty()                                                                                                                            // known_indels_tbi
+                input[21] = channel.empty()                                                                                                                            // known_snps
+                input[22] = channel.empty()                                                                                                                            // known_snps_tbi
                 input[23] = null                                                                                                                                       // msisensor2_models
                 input[24] = null                                                                                                                                       // msisensorpro_scan
-                input[25] = Channel.empty()                                                                                                                            // pon
-                input[26] = Channel.empty()                                                                                                                            // pon_tbi
+                input[25] = channel.empty()                                                                                                                            // pon
+                input[26] = channel.empty()                                                                                                                            // pon_tbi
                 input[27] = 'bwa-mem'                                                                                                                                  // aligner
                 input[28] = 'mapping'                                                                                                                                  // step
                 input[29] = "no_tools"                                                                                                                                 // tools - bbsplit not enabled
@@ -138,27 +138,27 @@ nextflow_workflow {
                 input[3]  = null                                                                                                                                       // ascat_loci_rt
                 input[4]  = null                                                                                                                                       // bbsplit_fasta_list
                 input[5]  = mock_index_dir.toString()                                                                                                                  // bbsplit_index (directory)
-                input[6]  = Channel.empty()                                                                                                                            // bcftools_annotations
-                input[7]  = Channel.empty()                                                                                                                            // bcftools_annotations_tbi
+                input[6]  = channel.empty()                                                                                                                            // bcftools_annotations
+                input[7]  = channel.empty()                                                                                                                            // bcftools_annotations_tbi
                 input[8]  = null                                                                                                                                       // bwa
                 input[9]  = null                                                                                                                                       // bwamem2
                 input[10] = null                                                                                                                                       // chr_dir
-                input[11] = Channel.empty()                                                                                                                            // dbsnp
-                input[12] = Channel.empty()                                                                                                                            // dbsnp_tbi
+                input[11] = channel.empty()                                                                                                                            // dbsnp
+                input[12] = channel.empty()                                                                                                                            // dbsnp_tbi
                 input[13] = null                                                                                                                                       // dict
                 input[14] = null                                                                                                                                       // dragmap
-                input[15] = Channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
+                input[15] = channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
                 input[16] = null                                                                                                                                       // fasta_fai
-                input[17] = Channel.empty()                                                                                                                            // germline_resource
-                input[18] = Channel.empty()                                                                                                                            // germline_resource_tbi
-                input[19] = Channel.empty()                                                                                                                            // known_indels
-                input[20] = Channel.empty()                                                                                                                            // known_indels_tbi
-                input[21] = Channel.empty()                                                                                                                            // known_snps
-                input[22] = Channel.empty()                                                                                                                            // known_snps_tbi
+                input[17] = channel.empty()                                                                                                                            // germline_resource
+                input[18] = channel.empty()                                                                                                                            // germline_resource_tbi
+                input[19] = channel.empty()                                                                                                                            // known_indels
+                input[20] = channel.empty()                                                                                                                            // known_indels_tbi
+                input[21] = channel.empty()                                                                                                                            // known_snps
+                input[22] = channel.empty()                                                                                                                            // known_snps_tbi
                 input[23] = null                                                                                                                                       // msisensor2_models
                 input[24] = null                                                                                                                                       // msisensorpro_scan
-                input[25] = Channel.empty()                                                                                                                            // pon
-                input[26] = Channel.empty()                                                                                                                            // pon_tbi
+                input[25] = channel.empty()                                                                                                                            // pon
+                input[26] = channel.empty()                                                                                                                            // pon_tbi
                 input[27] = 'bwa-mem'                                                                                                                                  // aligner
                 input[28] = 'mapping'                                                                                                                                  // step
                 input[29] = "bbsplit"                                                                                                                                  // tools - enable bbsplit
@@ -197,27 +197,27 @@ nextflow_workflow {
                 input[3]  = null                                                                                                                                       // ascat_loci_rt
                 input[4]  = "${projectDir}/tests/csv/bbsplit_fasta_list.csv"                                                                                           // bbsplit_fasta_list
                 input[5]  = null                                                                                                                                       // bbsplit_index
-                input[6]  = Channel.empty()                                                                                                                            // bcftools_annotations
-                input[7]  = Channel.empty()                                                                                                                            // bcftools_annotations_tbi
+                input[6]  = channel.empty()                                                                                                                            // bcftools_annotations
+                input[7]  = channel.empty()                                                                                                                            // bcftools_annotations_tbi
                 input[8]  = null                                                                                                                                       // bwa
                 input[9]  = null                                                                                                                                       // bwamem2
                 input[10] = null                                                                                                                                       // chr_dir
-                input[11] = Channel.empty()                                                                                                                            // dbsnp
-                input[12] = Channel.empty()                                                                                                                            // dbsnp_tbi
+                input[11] = channel.empty()                                                                                                                            // dbsnp
+                input[12] = channel.empty()                                                                                                                            // dbsnp_tbi
                 input[13] = null                                                                                                                                       // dict
                 input[14] = null                                                                                                                                       // dragmap
-                input[15] = Channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
+                input[15] = channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
                 input[16] = null                                                                                                                                       // fasta_fai
-                input[17] = Channel.empty()                                                                                                                            // germline_resource
-                input[18] = Channel.empty()                                                                                                                            // germline_resource_tbi
-                input[19] = Channel.empty()                                                                                                                            // known_indels
-                input[20] = Channel.empty()                                                                                                                            // known_indels_tbi
-                input[21] = Channel.empty()                                                                                                                            // known_snps
-                input[22] = Channel.empty()                                                                                                                            // known_snps_tbi
+                input[17] = channel.empty()                                                                                                                            // germline_resource
+                input[18] = channel.empty()                                                                                                                            // germline_resource_tbi
+                input[19] = channel.empty()                                                                                                                            // known_indels
+                input[20] = channel.empty()                                                                                                                            // known_indels_tbi
+                input[21] = channel.empty()                                                                                                                            // known_snps
+                input[22] = channel.empty()                                                                                                                            // known_snps_tbi
                 input[23] = null                                                                                                                                       // msisensor2_models
                 input[24] = null                                                                                                                                       // msisensorpro_scan
-                input[25] = Channel.empty()                                                                                                                            // pon
-                input[26] = Channel.empty()                                                                                                                            // pon_tbi
+                input[25] = channel.empty()                                                                                                                            // pon
+                input[26] = channel.empty()                                                                                                                            // pon_tbi
                 input[27] = 'bwa-mem'                                                                                                                                  // aligner
                 input[28] = 'mapping'                                                                                                                                  // step
                 input[29] = "bbsplit"                                                                                                                                  // tools - enable bbsplit
@@ -259,27 +259,27 @@ nextflow_workflow {
                 input[3]  = null                                                                                                                                       // ascat_loci_rt
                 input[4]  = null                                                                                                                                       // bbsplit_fasta_list
                 input[5]  = dummy_tar.toString()                                                                                                                       // bbsplit_index (tar.gz file)
-                input[6]  = Channel.empty()                                                                                                                            // bcftools_annotations
-                input[7]  = Channel.empty()                                                                                                                            // bcftools_annotations_tbi
+                input[6]  = channel.empty()                                                                                                                            // bcftools_annotations
+                input[7]  = channel.empty()                                                                                                                            // bcftools_annotations_tbi
                 input[8]  = null                                                                                                                                       // bwa
                 input[9]  = null                                                                                                                                       // bwamem2
                 input[10] = null                                                                                                                                       // chr_dir
-                input[11] = Channel.empty()                                                                                                                            // dbsnp
-                input[12] = Channel.empty()                                                                                                                            // dbsnp_tbi
+                input[11] = channel.empty()                                                                                                                            // dbsnp
+                input[12] = channel.empty()                                                                                                                            // dbsnp_tbi
                 input[13] = null                                                                                                                                       // dict
                 input[14] = null                                                                                                                                       // dragmap
-                input[15] = Channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
+                input[15] = channel.of([[id: "fasta"], file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)])        // fasta
                 input[16] = null                                                                                                                                       // fasta_fai
-                input[17] = Channel.empty()                                                                                                                            // germline_resource
-                input[18] = Channel.empty()                                                                                                                            // germline_resource_tbi
-                input[19] = Channel.empty()                                                                                                                            // known_indels
-                input[20] = Channel.empty()                                                                                                                            // known_indels_tbi
-                input[21] = Channel.empty()                                                                                                                            // known_snps
-                input[22] = Channel.empty()                                                                                                                            // known_snps_tbi
+                input[17] = channel.empty()                                                                                                                            // germline_resource
+                input[18] = channel.empty()                                                                                                                            // germline_resource_tbi
+                input[19] = channel.empty()                                                                                                                            // known_indels
+                input[20] = channel.empty()                                                                                                                            // known_indels_tbi
+                input[21] = channel.empty()                                                                                                                            // known_snps
+                input[22] = channel.empty()                                                                                                                            // known_snps_tbi
                 input[23] = null                                                                                                                                       // msisensor2_models
                 input[24] = null                                                                                                                                       // msisensorpro_scan
-                input[25] = Channel.empty()                                                                                                                            // pon
-                input[26] = Channel.empty()                                                                                                                            // pon_tbi
+                input[25] = channel.empty()                                                                                                                            // pon
+                input[26] = channel.empty()                                                                                                                            // pon_tbi
                 input[27] = 'bwa-mem'                                                                                                                                  // aligner
                 input[28] = 'mapping'                                                                                                                                  // step
                 input[29] = "bbsplit"                                                                                                                                  // tools - enable bbsplit
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index 8b7d2eff19..581612e465 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -22,20 +22,20 @@ workflow PREPARE_INTERVALS {
     step
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
-    intervals_bed        = Channel.empty() // List of [ bed, num_intervals ], one for each region
-    intervals_bed_gz_tbi = Channel.empty() // List of [ bed.gz, bed,gz.tbi, num_intervals ], one for each region
-    intervals_combined   = Channel.empty() // Single bed file containing all intervals
+    intervals_bed        = channel.empty() // List of [ bed, num_intervals ], one for each region
+    intervals_bed_gz_tbi = channel.empty() // List of [ bed.gz, bed,gz.tbi, num_intervals ], one for each region
+    intervals_combined   = channel.empty() // Single bed file containing all intervals
 
     if (no_intervals) {
         file("${outdir}/no_intervals.bed").text        = "no_intervals\n"
         file("${outdir}/no_intervals.bed.gz").text     = "no_intervals\n"
         file("${outdir}/no_intervals.bed.gz.tbi").text = "no_intervals\n"
 
-        intervals_bed        = Channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ it, 0 ] }
-        intervals_bed_gz_tbi = Channel.fromPath(file("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ it -> [ it, 0 ] }
-        intervals_combined   = Channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ [ id:it.simpleName ], it ] }
+        intervals_bed        = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ it, 0 ] }
+        intervals_bed_gz_tbi = channel.fromPath(file("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ it -> [ it, 0 ] }
+        intervals_combined   = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ [ id:it.simpleName ], it ] }
     } else if (step != 'annotate' && step != 'controlfreec') {
         // If no interval/target file is provided, then generated intervals from FASTA file
         if (!intervals) {
@@ -50,7 +50,7 @@ workflow PREPARE_INTERVALS {
             versions = versions.mix(BUILD_INTERVALS.out.versions)
             versions = versions.mix(CREATE_INTERVALS_BED.out.versions)
         } else {
-            intervals_combined = Channel.fromPath(file(intervals)).map{it -> [ [ id:it.baseName ], it ] }
+            intervals_combined = channel.fromPath(file(intervals)).map{it -> [ [ id:it.baseName ], it ] }
             CREATE_INTERVALS_BED(file(intervals), nucleotides_per_second)
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
diff --git a/subworkflows/local/prepare_reference_cnvkit/main.nf b/subworkflows/local/prepare_reference_cnvkit/main.nf
index 13bbef5f6b..9eaca9eff7 100644
--- a/subworkflows/local/prepare_reference_cnvkit/main.nf
+++ b/subworkflows/local/prepare_reference_cnvkit/main.nf
@@ -7,7 +7,7 @@ workflow PREPARE_REFERENCE_CNVKIT {
     intervals_bed_combined // channel: []
 
     main:
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // prepare a antitarget reference files for tumor_only mode of cnvkit
     CNVKIT_ANTITARGET(intervals_bed_combined.flatten().map { it -> [[id: 'intervals'], it] })
diff --git a/subworkflows/local/samplesheet_to_channel/tests/main.nf.test b/subworkflows/local/samplesheet_to_channel/tests/main.nf.test
index 32bbb3a8e8..21483b1861 100644
--- a/subworkflows/local/samplesheet_to_channel/tests/main.nf.test
+++ b/subworkflows/local/samplesheet_to_channel/tests/main.nf.test
@@ -11,7 +11,7 @@ nextflow_workflow {
             workflow {
                 """
                 // define inputs of the workflow here. Example:
-                input[0] = Channel.of([
+                input[0] = channel.of([
                     ['patient':'test', 'sample':'test',
                     'sex':'XX', 'status':0, 'lane':'test_L1'],
                     file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz', checkIfExists: true),
diff --git a/subworkflows/local/vcf_consensus/main.nf b/subworkflows/local/vcf_consensus/main.nf
index 0b9dbb028f..291d360684 100644
--- a/subworkflows/local/vcf_consensus/main.nf
+++ b/subworkflows/local/vcf_consensus/main.nf
@@ -12,7 +12,7 @@ workflow CONSENSUS {
     vcfs     // [meta, vcf ,tbi]
 
     main:
-    ch_versions = Channel.empty()
+    ch_versions = channel.empty()
 
     ch_vcfs = vcfs
         .branch{ meta, vcf, tbi ->
diff --git a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
index 21e4ce927a..85aa418968 100644
--- a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
+++ b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
@@ -10,7 +10,7 @@ workflow VCF_QC_BCFTOOLS_VCFTOOLS {
 
     main:
 
-    versions = Channel.empty()
+    versions = channel.empty()
 
     BCFTOOLS_STATS(vcf.map{ meta, vcf_ -> [ meta, vcf_, [] ] }, [[:],[]], [[:],[]], [[:],[]], [[:],[]], [[:],[]])
     VCFTOOLS_TSTV_COUNT(vcf, target_bed, [])
diff --git a/subworkflows/local/vcf_variant_filtering_gatk/main.nf b/subworkflows/local/vcf_variant_filtering_gatk/main.nf
index ce2b749c35..de98177777 100644
--- a/subworkflows/local/vcf_variant_filtering_gatk/main.nf
+++ b/subworkflows/local/vcf_variant_filtering_gatk/main.nf
@@ -14,7 +14,7 @@ workflow VCF_VARIANT_FILTERING_GATK {
 
     main:
 
-    versions = Channel.empty()
+    versions = channel.empty()
 
     // Don't scatter/gather by intervals, because especially for small regions (targeted or WGS), it easily fails with 0 SNPS in region
     cnn_in = vcf.combine(intervals_bed_combined).map{ meta, vcf_, tbi, intervals -> [ meta, vcf_, tbi, [], intervals ] }

From e17a658185de12849e7ec6448b07c363f731c6dd Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Thu, 16 Jul 2026 16:29:22 +0200
Subject: [PATCH 10/27] Replace deprecated `channel.from` with `channel.of` in
 prepare_genome (#2233)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Replace the last remaining `channel.from` call with `channel.of` in
`subworkflows/local/prepare_genome/main.nf`.

`channel.from` is deprecated in favour of
`channel.of`/`channel.fromList` and prints a runtime deprecation
warning. It is not flagged by `nextflow lint` (only the
capital-`Channel` namespace is), and it does not break on 25.10 or 26.x
— this is deprecation cleanup, not a compatibility fix:
[https://docs.seqera.io/nextflow/tutorials/static-types#avoid-deprecated-patterns](https://docs.seqera.io/nextflow/tutorials/static-types#avoid-deprecated-patterns)

## Changes

- `subworkflows/local/prepare_genome/main.nf` line 132:
`channel.from(...)` → `channel.of(...)`
- `CHANGELOG.md`: added entry under `### Changed`

## Semantics

The argument is a single file object (`file(bbsplit_fasta_list_in,
checkIfExists: true)` returns a single Path), so `channel.of` is a
direct drop-in with identical behaviour. The downstream `.splitCsv()`
chain is unaffected.
---
 CHANGELOG.md                              | 1 +
 subworkflows/local/prepare_genome/main.nf | 2 +-
 2 files changed, 2 insertions(+), 1 deletion(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f4e56870f5..26e44d9763 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -63,6 +63,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2178](https://github.com/nf-core/sarek/pull/2178) - Template update for nf-core/tools v4.0.2
 - [#2225](https://github.com/nf-core/sarek/pull/2225) - Back to dev (3.9.1dev)
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update nft-utils to 1.0.0, migrate `getAllFilesFromDir` to `getAllFilesFromPath` in test utilities
+- [#2233](https://github.com/nf-core/sarek/pull/2233) - Replace the last remaining deprecated `channel.from` with `channel.of` in `prepare_genome`
 
 #### Fixed
 
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index a78e0706da..192383baa7 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -129,7 +129,7 @@ workflow PREPARE_GENOME {
         }
         else if (bbsplit_fasta_list_in) {
             // Build it from scratch if we have FASTA
-            channel.from(file(bbsplit_fasta_list_in, checkIfExists: true))
+            channel.of(file(bbsplit_fasta_list_in, checkIfExists: true))
                 .splitCsv(header: false, sep: ',')
                 .flatMap { id, fafile -> [['id', id], ['fasta', file(fafile, checkIfExists: true)]] }
                 .groupTuple()

From e3d53c53b88bca2927b017597bfd072b03bdb8d0 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Fri, 17 Jul 2026 11:34:26 +0200
Subject: [PATCH 11/27] Replace `.set {}` with plain assignment for
 strict-syntax readiness (#2234)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Replaces the deprecated `.set {}` channel terminator with plain leading
assignment in four local subworkflows. This pattern still runs in
Nextflow
26.x but is flagged by the static-types type checker, so this is part of
the
granular strict-syntax cleanup series.

Pure mechanical, semantics-preserving change (`.set { name }` → `name =
<chain>`):

- `subworkflows/local/prepare_snpsift_databases/main.nf` — `ch_branched`
- `subworkflows/local/fastq_preprocess_gatk/main.nf` —
`reads_grouping_key`
- `subworkflows/local/fastq_preprocess_parabricks/main.nf` —
`reads_grouping_key`
- `subworkflows/local/prepare_genome/main.nf` — `ch_bbsplit_fasta_list`

## PR checklist

- [x] Targets the `dev` branch
- [x] `CHANGELOG.md` updated (Developer section)
- [ ] No functional/output changes — no tests or docs affected

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                           | 1 +
 subworkflows/local/fastq_preprocess_gatk/main.nf       | 3 +--
 subworkflows/local/fastq_preprocess_parabricks/main.nf | 4 ++--
 subworkflows/local/prepare_genome/main.nf              | 3 +--
 subworkflows/local/prepare_snpsift_databases/main.nf   | 4 ++--
 5 files changed, 7 insertions(+), 8 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 26e44d9763..568e07f50f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -64,6 +64,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2225](https://github.com/nf-core/sarek/pull/2225) - Back to dev (3.9.1dev)
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update nft-utils to 1.0.0, migrate `getAllFilesFromDir` to `getAllFilesFromPath` in test utilities
 - [#2233](https://github.com/nf-core/sarek/pull/2233) - Replace the last remaining deprecated `channel.from` with `channel.of` in `prepare_genome`
+- [#2234](https://github.com/nf-core/sarek/pull/2234) - Replace deprecated `.set {}` channel terminator with plain assignment in local subworkflows for Nextflow strict-syntax / 26.x readiness
 
 #### Fixed
 
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index 80dad07bdd..f1ea54f64e 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -157,14 +157,13 @@ workflow FASTQ_PREPROCESS_GATK {
         // First, we must calculate number of lanes for each sample (meta.n_fastq)
         // This is needed to group reads from the same sample together using groupKey to avoid stalling the workflow
         // when reads from different samples are mixed together
-        reads_for_alignment.map { meta, reads ->
+        reads_grouping_key = reads_for_alignment.map { meta, reads ->
                 [ meta.subMap('patient', 'sample', 'sex', 'status'), reads ]
             }
             .groupTuple()
             .map { meta, reads ->
                 meta + [ n_fastq: reads.size() ] // We can drop the FASTQ files now that we know how many there are
             }
-            .set { reads_grouping_key }
 
         reads_for_alignment = reads_for_alignment.map{ meta, reads ->
             // Update meta.id to meta.sample no multiple lanes or splitted fastqs
diff --git a/subworkflows/local/fastq_preprocess_parabricks/main.nf b/subworkflows/local/fastq_preprocess_parabricks/main.nf
index 805bc5af59..8886d0611f 100644
--- a/subworkflows/local/fastq_preprocess_parabricks/main.nf
+++ b/subworkflows/local/fastq_preprocess_parabricks/main.nf
@@ -21,13 +21,13 @@ workflow FASTQ_PREPROCESS_PARABRICKS {
     ch_versions = channel.empty()
     ch_reports  = channel.empty()
 
-    ch_reads.map { meta, reads ->
+    reads_grouping_key = ch_reads.map { meta, reads ->
             [ meta.subMap('patient', 'sample', 'sex', 'status'), reads ]
         }
         .groupTuple()
         .map { meta, reads ->
             meta + [ n_fastq: reads.size() ] // We can drop the FASTQ files now that we know how many there are
-        }.set { reads_grouping_key }
+        }
 
     ch_reads = ch_reads.map{ meta, reads ->
         // Update meta.id to meta.sample no multiple lanes or splitted fastqs
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 192383baa7..d05b1f980b 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -129,13 +129,12 @@ workflow PREPARE_GENOME {
         }
         else if (bbsplit_fasta_list_in) {
             // Build it from scratch if we have FASTA
-            channel.of(file(bbsplit_fasta_list_in, checkIfExists: true))
+            ch_bbsplit_fasta_list = channel.of(file(bbsplit_fasta_list_in, checkIfExists: true))
                 .splitCsv(header: false, sep: ',')
                 .flatMap { id, fafile -> [['id', id], ['fasta', file(fafile, checkIfExists: true)]] }
                 .groupTuple()
                 .map { it -> it[1] }
                 .collect { [it] }
-                .set { ch_bbsplit_fasta_list }
 
             bbsplit_index = BBMAP_INDEX(
                 [[id: "build_index"], []],
diff --git a/subworkflows/local/prepare_snpsift_databases/main.nf b/subworkflows/local/prepare_snpsift_databases/main.nf
index 581a62d020..e524212d72 100644
--- a/subworkflows/local/prepare_snpsift_databases/main.nf
+++ b/subworkflows/local/prepare_snpsift_databases/main.nf
@@ -12,10 +12,10 @@ workflow PREPARE_SNPSIFT_DATABASES {
     ch_configs = channel.fromList(val_db_configs)
 
     // Branch: create vardb if not provided
-    ch_configs.branch {
+    ch_branched = ch_configs.branch {
         has_vardb: it.vardb != null
         needs_vardb: true
-    }.set { ch_branched }
+    }
 
     // Create vardbs for databases that need them
     // Convert semicolon-separated fields to comma-separated (SnpSift expects commas)

From abb83ad196f235dc860f82ef5c6481c59522564f Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Tue, 21 Jul 2026 17:34:27 +0200
Subject: [PATCH 12/27] Nextflow strict-syntax readiness for local code (+
 Manta output & germline CNVKIT cleanups) (#2235)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## What changed & why

Nextflow **strict-syntax / 26.x readiness** for sarek's own code, plus
two small functional improvements that surfaced along the way. `nextflow
lint` is now clean for all local `.nf` and `conf/` files (only vendored
`modules/nf-core/` warnings remain — those clear via module updates).

### 🟢 Mechanical — no behaviour change (safe to skim)
- **Closures now have explicit, named parameters** across
`subworkflows/local/`, `workflows/`, `main.nf` — replacing
implicit/generic `it` (e.g. `.branch { meta, vcf -> … }`; single-channel
maps named after the source channel, `dbsnp.map { dbsnp_ -> … }`). *Why:
strict syntax deprecates implicit `it`.*
- **`publishDir` `saveAs` closures** in `conf/modules/*.config` use an
explicit `{ filename -> … }`. *Why: same.*
- **Unused closure params prefixed `_`; 5 genuinely-unused `take:`
inputs removed** (`bam`, `dbsnp_vqsr` ×2, `intervals_bed_gz_tbi`,
`bcftools_columns`) with their call args. *Why: dead inputs / lint.*

### 🟡 Functional — worth a look
- **germline CNVKit now consumes the shared BAM instead of CRAM.** *Why:
`CNVKIT_BATCH` internally re-converts CRAM→BAM (slow), duplicating the
pipeline's `CRAM_TO_BAM`; germline now reuses that single conversion —
one conversion instead of two on paired runs.* Analysis output content
is unchanged.
- **`CRAM_TO_BAM` sets `ext.prefix` to the input basename** so the
converted BAM (and therefore the CNVKit output filenames) mirror the
source CRAM name. *Why: without it the BAM switch above would have
renamed cnvkit outputs to `meta.id`, dropping the `…recalibrated…`
provenance from the filenames. This keeps the existing naming.*
- **Manta now emits complete candidate VCF + tbi pairs**
(`candidate_small_indels_vcf(_tbi)`, `candidate_sv_vcf(_tbi)`) with
`variantcaller:'manta'` on all outputs, and names SV outputs by type
(`diploid_sv_vcf`, `tumor_sv_vcf`). *Why: candidates were previously
assigned but never emitted (some missing their tbi / variantcaller
tag).* Strelka's annotation input is unchanged.

### 🔵 Snapshots
- Updated cnvkit snapshots (`variant_calling_cnvkit`,
`variant_calling_all`): cnvkit output filenames now follow the
source-CRAM basename, and `samtools` is dropped from `CNVKIT_BATCH`
versions (both consequences of the BAM switch). **Filename renames only
— file md5 content is unchanged.** `save_output_as_bam` is unaffected
(its cnvkit input basename was already the source name).

**No new parameters. No output-path changes; cnvkit output filenames are
preserved via the `ext.prefix` above.**

Pairs with #2232 (the `channel` factory migration) as the closure-`it`
half of the strict-syntax work.

## PR checklist
- [x] Description of changes (with reason) above.
- [x] Code lints (`nextflow lint` — 0 errors, 0 local warnings).
- [x] `CHANGELOG.md` updated.
- [ ] Test suite: cnvkit snapshots updated for the BAM switch +
`ext.prefix` naming; CI re-running to confirm green.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   2 +
 conf/modules/aligner.config                   |   6 +-
 conf/modules/aligner_parabricks.config        |   8 +-
 conf/modules/annotate.config                  |   6 +-
 conf/modules/contamination.config             |   2 +-
 conf/modules/deepvariant.config               |   2 +-
 conf/modules/freebayes.config                 |   2 +-
 conf/modules/haplotypecaller.config           |   2 +-
 conf/modules/lofreq.config                    |   2 +-
 conf/modules/markduplicates.config            |  16 +-
 conf/modules/modules.config                   |   3 +
 conf/modules/mpileup.config                   |   2 +-
 conf/modules/mutect2.config                   |   4 +-
 conf/modules/prepare_genome.config            |  36 +--
 conf/modules/prepare_intervals.config         |   6 +-
 conf/modules/prepare_recalibration.config     |   2 +-
 conf/modules/recalibrate.config               |  14 +-
 conf/modules/sentieon_dedup.config            |   6 +-
 conf/modules/sentieon_dnascope.config         |   2 +-
 conf/modules/sentieon_haplotyper.config       |   2 +-
 conf/modules/sentieon_tnscope.config          |   2 +-
 conf/modules/strelka.config                   |   2 +-
 docs/output.md                                |   3 +
 main.nf                                       |   8 +-
 modules/local/consensus_from_sites/main.nf    |   1 -
 .../local/bam_baserecalibrator/main.nf        |   6 +-
 .../local/bam_baserecalibrator_spark/main.nf  |   8 +-
 .../bam_joint_calling_germline_gatk/main.nf   |   2 +-
 subworkflows/local/bam_markduplicates/main.nf |   2 +-
 .../local/bam_markduplicates_spark/main.nf    |   4 +-
 .../bam_variant_calling_deepvariant/main.nf   |  18 +-
 .../bam_variant_calling_freebayes/main.nf     |   6 +-
 .../bam_variant_calling_germline_all/main.nf  |  41 +--
 .../main.nf                                   |  30 ++-
 .../main.nf                                   |  20 +-
 .../bam_variant_calling_indexcov/main.nf      |   2 +-
 .../local/bam_variant_calling_mpileup/main.nf |  18 +-
 .../main.nf                                   |  35 ++-
 .../main.nf                                   |  29 +--
 .../main.nf                                   |  18 +-
 .../bam_variant_calling_single_tiddit/main.nf |   4 +-
 .../bam_variant_calling_somatic_all/main.nf   |   7 +-
 .../main.nf                                   |   4 +-
 .../bam_variant_calling_somatic_manta/main.nf |  35 +--
 .../main.nf                                   |  44 ++--
 .../main.nf                                   |  24 +-
 .../main.nf                                   |  12 +-
 .../main.nf                                   |   7 +-
 .../main.nf                                   |  12 +-
 .../main.nf                                   |  30 ++-
 .../main.nf                                   |  38 +--
 .../main.nf                                   |  12 +-
 .../local/cram_qc_mosdepth_samtools/main.nf   |   2 +-
 subworkflows/local/prepare_genome/main.nf     |   8 +-
 subworkflows/local/prepare_intervals/main.nf  |  24 +-
 .../local/prepare_reference_cnvkit/main.nf    |   2 +-
 .../local/prepare_snpsift_databases/main.nf   |  20 +-
 .../local/samplesheet_to_channel/main.nf      |  21 +-
 .../local/utils_nfcore_sarek_pipeline/main.nf |   1 -
 subworkflows/local/vcf_consensus/main.nf      |   8 +-
 .../local/vcf_varlociraptor_somatic/main.nf   |  12 +-
 tests/variant_calling_all.nf.test.snap        |   6 +-
 tests/variant_calling_cnvkit.nf.test.snap     | 246 +++++++++---------
 tests/variant_calling_muse.nf.test.snap       |   2 +-
 workflows/sarek.nf                            |  37 ++-
 65 files changed, 501 insertions(+), 497 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 568e07f50f..122f2710a1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -16,6 +16,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2210](https://github.com/nf-core/sarek/pull/2210) - Update bcftools to 1.23.1
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update EnsemblVEP to 116.0
 - [#2232](https://github.com/nf-core/sarek/pull/2232) - Migrate local code to the lowercase `channel` factory for Nextflow strict-syntax / 26.x readiness
+- [#2235](https://github.com/nf-core/sarek/pull/2235) - Nextflow strict-syntax / 26.x readiness for local code: explicit, named closure parameters (replacing implicit/generic `it`), `_`-prefixed unused parameters, and removal of unused `take:` inputs. Previously-dropped Manta candidate VCFs and Sentieon gVCF indices are now emitted.
+- [#2235](https://github.com/nf-core/sarek/pull/2235) - germline CNVKIT reuses the shared `CRAM_TO_BAM` conversion instead of re-converting CRAM internally, avoiding a duplicate conversion. Side effect: with `--step variant_calling` (user-supplied CRAM/BAM), CNVKit output files are named after the input file rather than the sample; runs from FASTQ are unaffected.
 
 ### Fixed
 
diff --git a/conf/modules/aligner.config b/conf/modules/aligner.config
index 2af1fa8047..7ae23be9f3 100644
--- a/conf/modules/aligner.config
+++ b/conf/modules/aligner.config
@@ -41,14 +41,14 @@ process {
                 // Only save if save_output_as_bam AND
                 // (save_mapped OR no_markduplicates OR sentieon_dedup) AND
                 // only a single BAM file per sample
-                saveAs: {
+                saveAs: { filename ->
                     if (params.save_output_as_bam &&
                         (
                             params.save_mapped ||
                             (params.skip_tools && params.skip_tools.split(',').contains('markduplicates')) &&
                             !(params.tools && params.tools.split(',').contains('sentieon_dedup'))
                         ) && (meta.size * meta.num_lanes == 1)
-                    ) { "mapped/${meta.id}/${it}" }
+                    ) { "mapped/${meta.id}/${filename}" }
                     else { null }
                 }
             ]
@@ -78,7 +78,7 @@ process {
             path: { "${params.outdir}/preprocessing/" },
             pattern: "*{bam,bai}",
             // Only save if (save_output_as_bam AND (no_markduplicates OR save_mapped ))
-            saveAs: { (params.save_output_as_bam && (params.save_mapped || params.skip_tools && params.skip_tools.split(',').contains('markduplicates'))) ? "mapped/${meta.id}/${it}" : null }
+            saveAs: { filename -> (params.save_output_as_bam && (params.save_mapped || params.skip_tools && params.skip_tools.split(',').contains('markduplicates'))) ? "mapped/${meta.id}/${filename}" : null }
         ]
     }
 
diff --git a/conf/modules/aligner_parabricks.config b/conf/modules/aligner_parabricks.config
index 4d680e907a..adc7c7d91a 100644
--- a/conf/modules/aligner_parabricks.config
+++ b/conf/modules/aligner_parabricks.config
@@ -28,7 +28,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/parabricks/${meta.id}/" },
             pattern: "*{cram,crai}",
-            saveAs: { params.save_mapped ? it : null }
+            saveAs: { filename -> params.save_mapped ? filename : null }
         ]
     }
 
@@ -38,7 +38,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/parabricks/${meta.id}/" },
             pattern: "*cram",
-            saveAs: { params.save_mapped ? it : null }
+            saveAs: { filename -> params.save_mapped ? filename : null }
         ]
     }
 
@@ -47,7 +47,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/parabricks/${meta.id}/" },
             pattern: "*{cram,crai}",
-            saveAs: { params.save_mapped ? it : null }
+            saveAs: { filename -> params.save_mapped ? filename : null }
         ]
     }
 
@@ -56,7 +56,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/parabricks/${meta.id}/" },
             pattern: "*{bam,bam.bai}",
-            saveAs: { params.save_output_as_bam ? it : null }
+            saveAs: { filename -> params.save_output_as_bam ? filename : null }
         ]
     }
 }
diff --git a/conf/modules/annotate.config b/conf/modules/annotate.config
index 00ae12a2f8..35a160b4d2 100644
--- a/conf/modules/annotate.config
+++ b/conf/modules/annotate.config
@@ -24,7 +24,7 @@ process {
                     mode: params.publish_dir_mode,
                     path: { "${params.outdir}/reports/snpeff/${meta.variantcaller}/${meta.id}/" },
                     pattern: "*{csv,html,genes.txt}",
-                    saveAs: { params.tools.split(',').contains('snpeff') ? it : null }
+                    saveAs: { filename -> params.tools.split(',').contains('snpeff') ? filename : null }
                 ]
             ]
         }
@@ -85,7 +85,7 @@ process {
         publishDir = [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/reference/snpsift" },
-                saveAs: { params.save_reference ? it : null }
+                saveAs: { filename -> params.save_reference ? filename : null }
             ]
     }
 
@@ -123,7 +123,7 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/annotation/${meta.variantcaller}/${meta.id}/" },
                 pattern: "*{gz,gz.tbi}",
-                saveAs: { params.tools.split(',').contains('snpeff') ? it : null }
+                saveAs: { filename -> params.tools.split(',').contains('snpeff') ? filename : null }
             ]
         }
 }
diff --git a/conf/modules/contamination.config b/conf/modules/contamination.config
index 0cb9406519..f2f5929839 100644
--- a/conf/modules/contamination.config
+++ b/conf/modules/contamination.config
@@ -14,7 +14,7 @@ process {
                 path: { params.save_bbsplit_reads ? "${params.outdir}/preprocessing/bbsplit/${meta.id}" : params.outdir },
                 mode: params.publish_dir_mode,
                 pattern: '*.fastq.gz',
-                saveAs: { params.save_bbsplit_reads ? it : null }
+                saveAs: { filename -> params.save_bbsplit_reads ? filename : null }
             ]
         ]
     }
diff --git a/conf/modules/deepvariant.config b/conf/modules/deepvariant.config
index 6d97477550..6f75489482 100644
--- a/conf/modules/deepvariant.config
+++ b/conf/modules/deepvariant.config
@@ -26,7 +26,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/" },
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "deepvariant/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "deepvariant/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/freebayes.config b/conf/modules/freebayes.config
index 7a1ec2a1c5..26deda7b42 100644
--- a/conf/modules/freebayes.config
+++ b/conf/modules/freebayes.config
@@ -40,7 +40,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/" },
             pattern: "*vcf.gz",
-            saveAs: { meta.num_intervals > 1 ? null : "freebayes/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "freebayes/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/haplotypecaller.config b/conf/modules/haplotypecaller.config
index 7d652e8082..f3b83ece33 100644
--- a/conf/modules/haplotypecaller.config
+++ b/conf/modules/haplotypecaller.config
@@ -26,7 +26,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/"},
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "haplotypecaller/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "haplotypecaller/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/lofreq.config b/conf/modules/lofreq.config
index 5517fa0157..fde84fdb59 100644
--- a/conf/modules/lofreq.config
+++ b/conf/modules/lofreq.config
@@ -23,7 +23,7 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/variant_calling/" },
                 pattern: "*{vcf.gz,vcf.gz.tbi}",
-                saveAs: { meta.num_intervals > 1 ? null : "lofreq/${meta.id}/${it}" }
+                saveAs: { filename -> meta.num_intervals > 1 ? null : "lofreq/${meta.id}/${filename}" }
             ]
         }
 
diff --git a/conf/modules/markduplicates.config b/conf/modules/markduplicates.config
index ce69d39994..9a0b81245b 100644
--- a/conf/modules/markduplicates.config
+++ b/conf/modules/markduplicates.config
@@ -39,7 +39,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/mapped/${meta.id}/" },
             pattern: "*{cram,crai}",
-            saveAs: { !params.save_output_as_bam ? it : null }
+            saveAs: { filename -> !params.save_output_as_bam ? filename : null }
         ]
     }
 
@@ -71,7 +71,7 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{cram,crai}",
-                saveAs: { !params.save_output_as_bam ? it : null }
+                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
             ],
             [
                 mode: params.publish_dir_mode,
@@ -79,13 +79,13 @@ process {
                 // not samtools-style (test.md.bam.bai); rename on publish so users see the standard naming.
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{md.bam,md.bai}",
-                saveAs: { params.save_output_as_bam ? (it.endsWith('.md.bai') ? it.replace('.md.bai', '.md.bam.bai') : it) : null }
+                saveAs: { filename -> params.save_output_as_bam ? (filename.endsWith('.md.bai') ? filename.replace('.md.bai', '.md.bam.bai') : filename) : null }
             ],
             [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/reports/" },
                 pattern: "*metrics",
-                saveAs: { !(params.skip_tools && params.skip_tools.split(',').contains('markduplicates_report')) ? "markduplicates/${meta.id}/${it}" : null}
+                saveAs: { filename -> !(params.skip_tools && params.skip_tools.split(',').contains('markduplicates_report')) ? "markduplicates/${meta.id}/${filename}" : null}
             ]
         ]
     }
@@ -99,13 +99,13 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{cram,crai}",
-                saveAs: { !params.save_output_as_bam ? it : null }
+                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
             ],
             [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{md.bam,md.bam.bai}",
-                saveAs: { params.save_output_as_bam ? it : null }
+                saveAs: { filename -> params.save_output_as_bam ? filename : null }
             ]
         ]
     }
@@ -116,13 +116,13 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{cram,crai}",
-                saveAs: { !params.save_output_as_bam ? it : null }
+                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
             ],
             [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
                 pattern: "*{bam,bai}",
-                saveAs: { params.save_output_as_bam ? it : null }
+                saveAs: { filename -> params.save_output_as_bam ? filename : null }
             ]
         ]
     }
diff --git a/conf/modules/modules.config b/conf/modules/modules.config
index 4251f71403..a74b22254e 100644
--- a/conf/modules/modules.config
+++ b/conf/modules/modules.config
@@ -30,6 +30,9 @@ process {
     }
 
     withName: 'NFCORE_SAREK:SAREK:CRAM_TO_BAM' {
+        // Name the converted BAM after the source CRAM (minus suffix) so downstream
+        // tools that derive names from the input file (e.g. CNVKit) keep their naming
+        ext.prefix = { "${input.baseName}" }
         publishDir = [
             enabled: params.save_output_as_bam,
             path: { "${params.outdir}/preprocessing/converted/cram_to_bam/${meta.id}" },
diff --git a/conf/modules/mpileup.config b/conf/modules/mpileup.config
index edd2199354..c808137333 100644
--- a/conf/modules/mpileup.config
+++ b/conf/modules/mpileup.config
@@ -30,7 +30,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/bcftools/${meta.id}/" },
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : it }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : filename }
         ]
     }
 
diff --git a/conf/modules/mutect2.config b/conf/modules/mutect2.config
index d12c476e17..c2fabb657b 100644
--- a/conf/modules/mutect2.config
+++ b/conf/modules/mutect2.config
@@ -23,7 +23,7 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/variant_calling/" },
                 pattern: "*{vcf.gz,vcf.gz.tbi,stats}",
-                saveAs: { meta.num_intervals > 1 ? null : "mutect2/${meta.id}/${it}" }
+                saveAs: { filename -> meta.num_intervals > 1 ? null : "mutect2/${meta.id}/${filename}" }
             ]
         }
 
@@ -95,7 +95,7 @@ process {
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/variant_calling/" },
                 pattern: "*.table",
-                saveAs: { meta.num_intervals > 1 ? null : "mutect2/${meta.id}/${it}" }
+                saveAs: { filename -> meta.num_intervals > 1 ? null : "mutect2/${meta.id}/${filename}" }
             ]
         }
 
diff --git a/conf/modules/prepare_genome.config b/conf/modules/prepare_genome.config
index 5a84ea0a1f..cf045029f5 100644
--- a/conf/modules/prepare_genome.config
+++ b/conf/modules/prepare_genome.config
@@ -18,7 +18,7 @@ process {
         publishDir = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -28,7 +28,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "bwa",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -37,7 +37,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "bwamem2",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -47,7 +47,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "*{bed}",
-            saveAs: { params.save_reference || params.build_only_index ? "cnvkit/${it}" : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? "cnvkit/${filename}" : null }
         ]
     }
 
@@ -58,7 +58,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "*{cnn}",
-            saveAs: { params.save_reference || params.build_only_index ? "cnvkit/${it}" : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? "cnvkit/${filename}" : null }
         ]
     }
 
@@ -67,7 +67,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "dragmap",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -76,7 +76,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/dict" },
             pattern: "*dict",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -85,7 +85,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/msisensor2" },
             pattern: "*scan",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -94,7 +94,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/msisensorpro" },
             pattern: "*list",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -103,7 +103,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/fai" },
             pattern: "*fai",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -112,7 +112,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/bcfann" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -121,7 +121,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/dbsnp" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -130,7 +130,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/germline_resource" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -139,7 +139,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/known_indels" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -148,7 +148,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/known_snps" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -157,7 +157,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/pon" },
             pattern: "*vcf.gz.tbi",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 
@@ -172,7 +172,7 @@ process {
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/" },
-            saveAs: { (params.save_reference || params.build_only_index) && !it.equals('versions.yml') ? it : null }
+            saveAs: { filename -> (params.save_reference || params.build_only_index) && !filename.equals('versions.yml') ? filename : null }
         ]
     }
 
@@ -183,7 +183,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/msisensor2" },
             pattern: "models",
-            saveAs: { params.save_reference || params.build_only_index ? it : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? filename : null }
         ]
     }
 }
diff --git a/conf/modules/prepare_intervals.config b/conf/modules/prepare_intervals.config
index 815903b996..1c3810435b 100644
--- a/conf/modules/prepare_intervals.config
+++ b/conf/modules/prepare_intervals.config
@@ -25,7 +25,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "*bed",
-            saveAs: { params.save_reference || params.build_only_index ? "intervals/${it}" : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? "intervals/${filename}" : null }
         ]
     }
 
@@ -34,7 +34,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "*bed",
-            saveAs: { params.save_reference || params.build_only_index ? "intervals/${it}" : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? "intervals/${filename}" : null }
         ]
     }
 
@@ -44,7 +44,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference" },
             pattern: "*bed.gz",
-            saveAs: { params.save_reference || params.build_only_index ? "intervals/${it}" : null }
+            saveAs: { filename -> params.save_reference || params.build_only_index ? "intervals/${filename}" : null }
         ]
     }
 }
diff --git a/conf/modules/prepare_recalibration.config b/conf/modules/prepare_recalibration.config
index d9c74fe044..98bcd9408d 100644
--- a/conf/modules/prepare_recalibration.config
+++ b/conf/modules/prepare_recalibration.config
@@ -22,7 +22,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/" },
             pattern: "*table",
-            saveAs: { meta.num_intervals > 1 ? null : "recal_table/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "recal_table/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/recalibrate.config b/conf/modules/recalibrate.config
index 779c7aefde..9342cc2e9c 100644
--- a/conf/modules/recalibrate.config
+++ b/conf/modules/recalibrate.config
@@ -25,19 +25,19 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/" },
             pattern: "*recal.cram",
-            saveAs: { !params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${it}" : null }
+            saveAs: { filename -> !params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename}" : null }
         ],
         [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/" },
             pattern: "*recal.bam",
-            saveAs: { params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${it}" : null }
+            saveAs: { filename -> params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename}" : null }
         ],
         [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/" },
             pattern: "*recal.bai",
-            saveAs: { params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${it.replace('.bai', '.bam.bai')}" : null }
+            saveAs: { filename -> params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename.replace('.bai', '.bam.bai')}" : null }
         ]]
     }
 
@@ -47,7 +47,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/recalibrated/${meta.id}/" },
             pattern: "*cram",
-            saveAs: { !params.save_output_as_bam ? it : null }
+            saveAs: { filename -> !params.save_output_as_bam ? filename : null }
         ]
     }
 
@@ -56,7 +56,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/recalibrated/${meta.id}/" },
             pattern: "*{recal.cram,recal.cram.crai}",
-            saveAs: { !params.save_output_as_bam ? it : null }
+            saveAs: { filename -> !params.save_output_as_bam ? filename : null }
         ]
     }
 
@@ -66,7 +66,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/recalibrated/${meta.id}/" },
             pattern: "*bam",
-            saveAs: { params.save_output_as_bam ? it : null }
+            saveAs: { filename -> params.save_output_as_bam ? filename : null }
         ]
     }
 
@@ -76,7 +76,7 @@ process {
             path: { "${params.outdir}/preprocessing/recalibrated/${meta.id}/" },
             pattern: "*{recal.bam,recal.bam.bai}",
             // single-interval BAM is already published by GATK4_APPLYBQSR; only publish the merged output here
-            saveAs: { (params.save_output_as_bam && meta.num_intervals > 1) ? it : null }
+            saveAs: { filename -> (params.save_output_as_bam && meta.num_intervals > 1) ? filename : null }
         ]
     }
 }
diff --git a/conf/modules/sentieon_dedup.config b/conf/modules/sentieon_dedup.config
index d0ff1add47..e514ad1164 100644
--- a/conf/modules/sentieon_dedup.config
+++ b/conf/modules/sentieon_dedup.config
@@ -32,19 +32,19 @@ process {
                 mode: params.publish_dir_mode,
                 path: { params.sentieon_consensus ? "${params.outdir}/preprocessing/sentieon_consensus/${meta.id}/" : "${params.outdir}/preprocessing/sentieon_dedup/${meta.id}/" },
                 pattern: "*{cram,crai}",
-                saveAs: { !params.save_output_as_bam ? it : null }
+                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
             ],
             [
                 mode: params.publish_dir_mode,
                 path: { params.sentieon_consensus ? "${params.outdir}/preprocessing/sentieon_consensus/${meta.id}/" : "${params.outdir}/preprocessing/sentieon_dedup/${meta.id}/" },
                 pattern: "*{bam,bai}",
-                saveAs: { params.save_output_as_bam ? it : null }
+                saveAs: { filename -> params.save_output_as_bam ? filename : null }
             ],
             [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/reports/" },
                 pattern: "*{metrics,metrics.multiqc.tsv}",
-                saveAs: { !(params.skip_tools && params.skip_tools.split(',').contains('sentieon_dedup_report')) ? "sentieon_dedup/${meta.id}/${it}" : null}
+                saveAs: { filename -> !(params.skip_tools && params.skip_tools.split(',').contains('sentieon_dedup_report')) ? "sentieon_dedup/${meta.id}/${filename}" : null}
             ]
         ]
     }
diff --git a/conf/modules/sentieon_dnascope.config b/conf/modules/sentieon_dnascope.config
index 50cf373ea8..42207dcec1 100644
--- a/conf/modules/sentieon_dnascope.config
+++ b/conf/modules/sentieon_dnascope.config
@@ -22,7 +22,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/"},
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "sentieon_dnascope/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "sentieon_dnascope/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/sentieon_haplotyper.config b/conf/modules/sentieon_haplotyper.config
index e3ce58194a..522ec70bd4 100644
--- a/conf/modules/sentieon_haplotyper.config
+++ b/conf/modules/sentieon_haplotyper.config
@@ -22,7 +22,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/"},
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "sentieon_haplotyper/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "sentieon_haplotyper/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/sentieon_tnscope.config b/conf/modules/sentieon_tnscope.config
index 6c9ded0a80..e8b185d27c 100644
--- a/conf/modules/sentieon_tnscope.config
+++ b/conf/modules/sentieon_tnscope.config
@@ -25,7 +25,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/"},
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "sentieon_tnscope/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "sentieon_tnscope/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/conf/modules/strelka.config b/conf/modules/strelka.config
index badffb5df8..177ec0fb77 100644
--- a/conf/modules/strelka.config
+++ b/conf/modules/strelka.config
@@ -23,7 +23,7 @@ process {
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/" },
             pattern: "*{vcf.gz,vcf.gz.tbi}",
-            saveAs: { meta.num_intervals > 1 ? null : "strelka/${meta.id}/${it}" }
+            saveAs: { filename -> meta.num_intervals > 1 ? null : "strelka/${meta.id}/${filename}" }
         ]
     }
 
diff --git a/docs/output.md b/docs/output.md
index 95c71913be..613966cb85 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -827,6 +827,9 @@ The file `<tumorsample_vs_normalsample>.cnvs.txt` contains all segments predicte
 
 [CNVKit](https://cnvkit.readthedocs.io/en/stable/) is a toolkit to infer and visualize copy number from high-throughput DNA sequencing data. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina. For further reading and documentation, see the [CNVKit Documentation](https://cnvkit.readthedocs.io/en/stable/plots.html)
 
+> [!NOTE]
+> When starting from `--step variant_calling` with a pre-aligned CRAM/BAM, CNVKit output **file names** follow the base name of the input file rather than the sample ID. Runs starting from FASTQ are unaffected — the sample-based names listed below apply. Output **directories** are always named after the sample (`<sample>` / `<tumorsample>_vs_<normalsample>`).
+
 <details markdown="1">
 <summary>Output files for normal and tumor-only samples</summary>
 
diff --git a/main.nf b/main.nf
index c03cc52266..2316687d8d 100755
--- a/main.nf
+++ b/main.nf
@@ -138,7 +138,7 @@ workflow NFCORE_SAREK {
 
     // For QC during preprocessing, we don't need any intervals (MOSDEPTH doesn't take them for WGS)
     intervals_for_preprocessing = params.wes
-        ? intervals_bed_combined.map { it -> [[id: it.baseName], it] }.collect()
+        ? intervals_bed_combined.map { bed -> [[id: bed.baseName], bed] }.collect()
         : channel.value([[id: 'null'], []])
     // [ interval, num_intervals ] multiple interval.bed files, divided by useful intervals for scatter/gather
     intervals = PREPARE_INTERVALS.out.intervals_bed
@@ -327,9 +327,9 @@ workflow NFCORE_SAREK {
         params.varlociraptor_events_somatic,
         params.varlociraptor_events_tumor_only,
         params.varlociraptor_fdr,
-        params.varlociraptor_scenario_germline ? channel.fromPath(params.varlociraptor_scenario_germline).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect(),
-        params.varlociraptor_scenario_somatic ? channel.fromPath(params.varlociraptor_scenario_somatic).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect(),
-        params.varlociraptor_scenario_tumor_only ? channel.fromPath(params.varlociraptor_scenario_tumor_only).map { it -> [[id: it.baseName - '.yte'], it] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect(),
+        params.varlociraptor_scenario_germline ? channel.fromPath(params.varlociraptor_scenario_germline).map { scenario -> [[id: scenario.baseName - '.yte'], scenario] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_germline.yte.yaml").collect(),
+        params.varlociraptor_scenario_somatic ? channel.fromPath(params.varlociraptor_scenario_somatic).map { scenario -> [[id: scenario.baseName - '.yte'], scenario] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_somatic.yte.yaml").collect(),
+        params.varlociraptor_scenario_tumor_only ? channel.fromPath(params.varlociraptor_scenario_tumor_only).map { scenario -> [[id: scenario.baseName - '.yte'], scenario] }.collect() : channel.fromPath("${projectDir}/assets/varlociraptor_tumor_only.yte.yaml").collect(),
         snpeff_cache,
         params.snpeff_db,
         vep_cache,
diff --git a/modules/local/consensus_from_sites/main.nf b/modules/local/consensus_from_sites/main.nf
index 510cf045e7..254e7cf056 100644
--- a/modules/local/consensus_from_sites/main.nf
+++ b/modules/local/consensus_from_sites/main.nf
@@ -24,7 +24,6 @@ process CONSENSUS_FROM_SITES {
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     def callers = meta.callers.join(',')
     """
diff --git a/subworkflows/local/bam_baserecalibrator/main.nf b/subworkflows/local/bam_baserecalibrator/main.nf
index 70563ab461..cc6f833a2d 100644
--- a/subworkflows/local/bam_baserecalibrator/main.nf
+++ b/subworkflows/local/bam_baserecalibrator/main.nf
@@ -36,10 +36,10 @@ workflow BAM_BASERECALIBRATOR {
     )
 
     // Figuring out if there is one or more table(s) from the same sample
-    table_to_merge = GATK4_BASERECALIBRATOR.out.table.map{ meta, table -> [ groupKey(meta, meta.num_intervals), table ] }.groupTuple().branch{
+    table_to_merge = GATK4_BASERECALIBRATOR.out.table.map{ meta, table -> [ groupKey(meta, meta.num_intervals), table ] }.groupTuple().branch{ meta, _table ->
         // Use meta.num_intervals to asses number of intervals
-        single:   it[0].num_intervals <= 1
-        multiple: it[0].num_intervals > 1
+        single:   meta.num_intervals <= 1
+        multiple: meta.num_intervals > 1
     }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_baserecalibrator_spark/main.nf b/subworkflows/local/bam_baserecalibrator_spark/main.nf
index 26a881b313..39ff897bed 100644
--- a/subworkflows/local/bam_baserecalibrator_spark/main.nf
+++ b/subworkflows/local/bam_baserecalibrator_spark/main.nf
@@ -26,13 +26,13 @@ workflow BAM_BASERECALIBRATOR_SPARK {
         .map{ meta, cram_, crai, intervals_, num_intervals -> [ meta + [ num_intervals:num_intervals ], cram_, crai, intervals_ ] }
 
     // RUN BASERECALIBRATOR SPARK
-    GATK4SPARK_BASERECALIBRATOR(cram_intervals, fasta.map{ meta, it -> [ it ] }, fasta_fai.map{ meta, it -> [ it ] }, dict.map{ meta, it -> [ it ] }, known_sites, known_sites_tbi)
+    GATK4SPARK_BASERECALIBRATOR(cram_intervals, fasta.map{ _meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ _meta, dict_ -> [ dict_ ] }, known_sites, known_sites_tbi)
 
     // Figuring out if there is one or more table(s) from the same sample
-    table_to_merge = GATK4SPARK_BASERECALIBRATOR.out.table.map{ meta, table -> [ groupKey(meta, meta.num_intervals), table ] }.groupTuple().branch{
+    table_to_merge = GATK4SPARK_BASERECALIBRATOR.out.table.map{ meta, table -> [ groupKey(meta, meta.num_intervals), table ] }.groupTuple().branch{ meta, _table ->
         // Use meta.num_intervals to asses number of intervals
-        single:   it[0].num_intervals <= 1
-        multiple: it[0].num_intervals > 1
+        single:   meta.num_intervals <= 1
+        multiple: meta.num_intervals > 1
     }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
index d7d04fc094..a9973ba218 100644
--- a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
+++ b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
@@ -52,7 +52,7 @@ workflow BAM_JOINT_CALLING_GERMLINE_GATK {
     // Joint genotyping performed using GenotypeGVCFs
     // Sort vcfs called by interval within each VCF
 
-    GATK4_GENOTYPEGVCFS(genotype_input, fasta, fai, dict, dbsnp.map{ it -> [ [:], it ] }, dbsnp_tbi.map{ it -> [ [:], it ] })
+    GATK4_GENOTYPEGVCFS(genotype_input, fasta, fai, dict, dbsnp.map{ dbsnp_ -> [ [:], dbsnp_ ] }, dbsnp_tbi.map{ dbsnp_tbi_ -> [ [:], dbsnp_tbi_ ] })
 
     BCFTOOLS_SORT(GATK4_GENOTYPEGVCFS.out.vcf)
     gvcf_to_merge = BCFTOOLS_SORT.out.vcf.map{ meta, vcf -> [ meta.subMap('num_intervals') + [ id:'joint_variant_calling', patient:'all_samples', variantcaller:'haplotypecaller' ], vcf ]}.groupTuple()
diff --git a/subworkflows/local/bam_markduplicates/main.nf b/subworkflows/local/bam_markduplicates/main.nf
index baed8ec73e..d3fb73f0f7 100644
--- a/subworkflows/local/bam_markduplicates/main.nf
+++ b/subworkflows/local/bam_markduplicates/main.nf
@@ -21,7 +21,7 @@ workflow BAM_MARKDUPLICATES {
     // RUN MARKUPDUPLICATES
     // --CREATE_INDEX true is set via ext.args when --save_output_as_bam, so the
     // module emits .bai inline; CRAM mode emits .crai via samtools post-conversion.
-    GATK4_MARKDUPLICATES(bam, fasta.map{ meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] })
+    GATK4_MARKDUPLICATES(bam, fasta.map{ _meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] })
 
     // Unified alignment output — BAM or CRAM depending on save_output_as_bam
     alignment = GATK4_MARKDUPLICATES.out.bam
diff --git a/subworkflows/local/bam_markduplicates_spark/main.nf b/subworkflows/local/bam_markduplicates_spark/main.nf
index e897b6088b..96de5aef24 100644
--- a/subworkflows/local/bam_markduplicates_spark/main.nf
+++ b/subworkflows/local/bam_markduplicates_spark/main.nf
@@ -22,7 +22,7 @@ workflow BAM_MARKDUPLICATES_SPARK {
     reports = channel.empty()
 
     // RUN MARKUPDUPLICATES SPARK
-    GATK4SPARK_MARKDUPLICATES(bam, fasta.map{ meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ meta, dict_ -> [ dict_ ] })
+    GATK4SPARK_MARKDUPLICATES(bam, fasta.map{ _meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ _meta, dict_ -> [ dict_ ] })
 
     // Index output (BAM or CRAM depending on ext.prefix)
     INDEX_MARKDUPLICATES(GATK4SPARK_MARKDUPLICATES.out.output)
@@ -35,7 +35,7 @@ workflow BAM_MARKDUPLICATES_SPARK {
     CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, intervals_bed_combined)
 
     // When running Marduplicates spark, and saving reports
-    GATK4_ESTIMATELIBRARYCOMPLEXITY(bam, fasta.map{ meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ meta, dict_ -> [ dict_ ] })
+    GATK4_ESTIMATELIBRARYCOMPLEXITY(bam, fasta.map{ _meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ _meta, dict_ -> [ dict_ ] })
 
     // Gather all reports generated
     reports = reports.mix(GATK4_ESTIMATELIBRARYCOMPLEXITY.out.metrics)
diff --git a/subworkflows/local/bam_variant_calling_deepvariant/main.nf b/subworkflows/local/bam_variant_calling_deepvariant/main.nf
index 9047358866..b5bd8d4f64 100644
--- a/subworkflows/local/bam_variant_calling_deepvariant/main.nf
+++ b/subworkflows/local/bam_variant_calling_deepvariant/main.nf
@@ -27,17 +27,17 @@ workflow BAM_VARIANT_CALLING_DEEPVARIANT {
     DEEPVARIANT_RUNDEEPVARIANT(cram_intervals, fasta, fasta_fai, [ [ id:'null' ], [] ], [ [ id:'null' ], [] ])
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_out = DEEPVARIANT_RUNDEEPVARIANT.out.vcf.branch{
+    vcf_out = DEEPVARIANT_RUNDEEPVARIANT.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more gvcf(s) from the same sample
-    gvcf_out = DEEPVARIANT_RUNDEEPVARIANT.out.gvcf.branch{
+    gvcf_out = DEEPVARIANT_RUNDEEPVARIANT.out.gvcf.branch{ meta, _gvcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -48,10 +48,10 @@ workflow BAM_VARIANT_CALLING_DEEPVARIANT {
     MERGE_DEEPVARIANT_VCF(vcf_to_merge, dict)
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_out = DEEPVARIANT_RUNDEEPVARIANT.out.vcf_index.branch{
+    tbi_out = DEEPVARIANT_RUNDEEPVARIANT.out.vcf_index.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Mix intervals and no_intervals channels together
diff --git a/subworkflows/local/bam_variant_calling_freebayes/main.nf b/subworkflows/local/bam_variant_calling_freebayes/main.nf
index a078c157a7..e2a8d4d452 100644
--- a/subworkflows/local/bam_variant_calling_freebayes/main.nf
+++ b/subworkflows/local/bam_variant_calling_freebayes/main.nf
@@ -32,10 +32,10 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     BCFTOOLS_SORT(FREEBAYES.out.vcf)
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    bcftools_vcf_out = BCFTOOLS_SORT.out.vcf.branch{
+    bcftools_vcf_out = BCFTOOLS_SORT.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index 7e9b5c9469..d0ff46289a 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -55,8 +55,10 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     versions = channel.empty()
 
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
-    gvcf_sentieon_dnascope   = channel.empty()
-    gvcf_sentieon_haplotyper = channel.empty()
+    gvcf_sentieon_dnascope       = channel.empty()
+    gvcf_sentieon_haplotyper     = channel.empty()
+    gvcf_tbi_sentieon_dnascope   = channel.empty()
+    gvcf_tbi_sentieon_haplotyper = channel.empty()
 
     out_indexcov             = channel.empty()
     vcf_deepvariant          = channel.empty()
@@ -96,11 +98,12 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     if (tools && tools.split(',').contains('cnvkit')) {
         BAM_VARIANT_CALLING_CNVKIT(
             // Remap channel to match module/subworkflow
-            cram.map{ meta, cram_, crai -> [ meta, [], cram_ ] },
+            // Use the already-converted BAM (shared with somatic/tumor-only) instead of CRAM
+            bam.map{ meta, bam_, _bai -> [ meta, [], bam_ ] },
             fasta,
             fasta_fai,
-            intervals_bed_combined.map{it -> it ? [[id:it[0].baseName], it]: [[id:'no_intervals'], []]},
-            params.cnvkit_reference ? cnvkit_reference.map{ it -> [[id:it[0].baseName], it] } : [[:],[]]
+            intervals_bed_combined.map{_intervals -> _intervals ? [[id:_intervals[0].baseName], _intervals]: [[id:'no_intervals'], []]},
+            params.cnvkit_reference ? cnvkit_reference.map{ reference -> [[id:reference[0].baseName], reference] } : [[:],[]]
         )
         versions = versions.mix(BAM_VARIANT_CALLING_CNVKIT.out.versions)
     }
@@ -143,8 +146,8 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             fasta,
             fasta_fai,
             dict,
-            dbsnp.map{it -> [[:], it]},
-            dbsnp_tbi.map{it -> [[:], it]},
+            dbsnp.map{dbsnp_ -> [[:], dbsnp_]},
+            dbsnp_tbi.map{dbsnp_tbi_ -> [[:], dbsnp_tbi_]},
             intervals)
 
         vcf_haplotypecaller = BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.vcf
@@ -178,9 +181,9 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
                 VCF_VARIANT_FILTERING_GATK(
                     vcf_haplotypecaller.join(tbi_haplotypecaller, failOnDuplicate: true, failOnMismatch: true),
-                    fasta.map{ meta, fasta_ -> [ fasta_ ] },
-                    fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] },
-                    dict.map{ meta, dict_ -> [ dict_ ] },
+                    fasta.map{ _meta, fasta_ -> [ fasta_ ] },
+                    fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] },
+                    dict.map{ _meta, dict_ -> [ dict_ ] },
                     intervals_bed_combined_haplotypec,
                     known_sites_indels.concat(known_sites_snps).flatten().unique().collect(),
                     known_sites_indels_tbi.concat(known_sites_snps_tbi).flatten().unique().collect())
@@ -202,8 +205,8 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             intervals_bed_gz_tbi_combined
         )
 
-        vcf_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.vcf
-        tbi_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.tbi
+        vcf_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.diploid_sv_vcf
+        tbi_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.diploid_sv_vcf_tbi
         versions = versions.mix(BAM_VARIANT_CALLING_GERMLINE_MANTA.out.versions)
     }
 
@@ -228,7 +231,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             dict,
             dbsnp,
             dbsnp_tbi,
-            dbsnp_vqsr,
             intervals,
             joint_germline,
             sentieon_dnascope_emit_mode,
@@ -288,7 +290,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             dict,
             dbsnp,
             dbsnp_tbi,
-            dbsnp_vqsr,
             intervals,
             joint_germline,
             sentieon_haplotyper_emit_mode)
@@ -327,9 +328,9 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
                 SENTIEON_HAPLOTYPER_VCF_VARIANT_FILTERING_GATK(
                     vcf_sentieon_haplotyper.join(tbi_sentieon_haplotyper, failOnDuplicate: true, failOnMismatch: true),
-                    fasta.map{ meta, it -> [ it ] },
-                    fasta_fai.map{ meta, it -> [ it ] },
-                    dict.map{ meta, dict_ -> [ dict_ ] },
+                    fasta.map{ _meta, fasta_ -> [ fasta_ ] },
+                    fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] },
+                    dict.map{ _meta, dict_ -> [ dict_ ] },
                     intervals_bed_combined_haplotypec,
                     known_sites_indels.concat(known_sites_snps).flatten().unique().collect(),
                     known_sites_indels_tbi.concat(known_sites_snps_tbi).flatten().unique().collect())
@@ -349,8 +350,8 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         BAM_VARIANT_CALLING_SINGLE_STRELKA(
             cram,
             dict,
-            fasta.map{ meta, fasta_ -> [ fasta_ ] },
-            fasta_fai.map{ meta, fasta_fai_ -> [ fasta_fai_ ] },
+            fasta.map{ _meta, fasta_ -> [ fasta_ ] },
+            fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] },
             intervals_bed_gz_tbi
         )
 
@@ -400,6 +401,8 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     emit:
     gvcf_sentieon_dnascope
     gvcf_sentieon_haplotyper
+    gvcf_tbi_sentieon_dnascope
+    gvcf_tbi_sentieon_haplotyper
     out_indexcov
     vcf_all
     vcf_deepvariant
diff --git a/subworkflows/local/bam_variant_calling_germline_manta/main.nf b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
index 1c086ec6ca..27289fb833 100644
--- a/subworkflows/local/bam_variant_calling_germline_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
@@ -18,30 +18,32 @@ workflow BAM_VARIANT_CALLING_GERMLINE_MANTA {
     versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
-    cram_intervals = cram.combine(intervals).map{ it ->
-        def bed_gz = it.size() > 3 ? it[3] : []
-        def bed_tbi = it.size() > 3 ? it[4] : []
+    cram_intervals = cram.combine(intervals).map{ combined ->
+        def bed_gz = combined.size() > 3 ? combined[3] : []
+        def bed_tbi = combined.size() > 3 ? combined[4] : []
 
-        [it[0], it[1], it[2], bed_gz, bed_tbi]
+        [combined[0], combined[1], combined[2], bed_gz, bed_tbi]
     }
 
     MANTA_GERMLINE(cram_intervals, fasta, fasta_fai, [])
 
-    small_indels_vcf     = MANTA_GERMLINE.out.candidate_small_indels_vcf
-    sv_vcf               = MANTA_GERMLINE.out.candidate_sv_vcf
-    diploid_sv_vcf       = MANTA_GERMLINE.out.diploid_sv_vcf
-    diploid_sv_vcf_tbi   = MANTA_GERMLINE.out.diploid_sv_vcf_tbi
-
-    // Only diploid SV should get annotated
     // add variantcaller to meta map
-    vcf = diploid_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
-    tbi = diploid_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_small_indels_vcf     = MANTA_GERMLINE.out.candidate_small_indels_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_small_indels_vcf_tbi = MANTA_GERMLINE.out.candidate_small_indels_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_sv_vcf               = MANTA_GERMLINE.out.candidate_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_sv_vcf_tbi           = MANTA_GERMLINE.out.candidate_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    diploid_sv_vcf                 = MANTA_GERMLINE.out.diploid_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    diploid_sv_vcf_tbi             = MANTA_GERMLINE.out.diploid_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
     versions = versions.mix(MANTA_GERMLINE.out.versions)
 
     emit:
-    vcf
-    tbi
+    candidate_small_indels_vcf
+    candidate_small_indels_vcf_tbi
+    candidate_sv_vcf
+    candidate_sv_vcf_tbi
+    diploid_sv_vcf
+    diploid_sv_vcf_tbi
 
     versions
 }
diff --git a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
index c700d29cfd..a05ee92e4d 100644
--- a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
+++ b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
@@ -42,34 +42,34 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
     gvcf_tbi_intervals = GATK4_HAPLOTYPECALLER.out.vcf
         .join(GATK4_HAPLOTYPECALLER.out.tbi, failOnMismatch: true)
         .join(cram_intervals, failOnMismatch: true)
-        .map{ meta, gvcf, tbi, _cram, _crai, intervals_, dragstr_model -> [ meta, gvcf, tbi, intervals_ ] }
+        .map{ meta, gvcf, tbi, _cram, _crai, intervals_, _dragstr_model -> [ meta, gvcf, tbi, intervals_ ] }
 
     // Figuring out if there is one or more vcf(s) from the same sample
     haplotypecaller_vcf = GATK4_HAPLOTYPECALLER.out.vcf.map{
             meta, vcf_ -> [ meta - meta.subMap('interval_name'), vcf_]
         }
-        .branch{
+        .branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     // Figuring out if there is one or more tbi(s) from the same sample
     haplotypecaller_tbi = GATK4_HAPLOTYPECALLER.out.tbi.map{
             meta, tbi -> [ meta - meta.subMap('interval_name'), tbi]
-        }.branch{
+        }.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     // Figuring out if there is one or more bam(s) from the same sample
     haplotypecaller_bam = GATK4_HAPLOTYPECALLER.out.bam.map{
             meta, bam -> [ meta - meta.subMap('interval_name'), bam]
-        }.branch{
+        }.branch{ meta, _bam ->
         // Use meta.num_intervals to asses number of intervals
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_variant_calling_indexcov/main.nf b/subworkflows/local/bam_variant_calling_indexcov/main.nf
index 2f13b44f56..4255de91cc 100644
--- a/subworkflows/local/bam_variant_calling_indexcov/main.nf
+++ b/subworkflows/local/bam_variant_calling_indexcov/main.nf
@@ -27,7 +27,7 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
     versions = versions.mix(reindex_ch.versions)
 
     // create [ [id:directory], bams, bais ]
-    indexcov_input_ch = reindex_ch.output.map{[[id:"indexcov"], it[1], it[2]]}.groupTuple()
+    indexcov_input_ch = reindex_ch.output.map{ _meta, bam, bai -> [[id:"indexcov"], bam, bai]}.groupTuple()
 
     goleft_ch = GOLEFT_INDEXCOV(
         indexcov_input_ch,
diff --git a/subworkflows/local/bam_variant_calling_mpileup/main.nf b/subworkflows/local/bam_variant_calling_mpileup/main.nf
index 1c601a4993..d46aa77b1b 100644
--- a/subworkflows/local/bam_variant_calling_mpileup/main.nf
+++ b/subworkflows/local/bam_variant_calling_mpileup/main.nf
@@ -38,21 +38,21 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     SAMTOOLS_MPILEUP(cram_intervals, fasta)
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_mpileup = BCFTOOLS_MPILEUP.out.vcf.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    vcf_mpileup = BCFTOOLS_MPILEUP.out.vcf.branch { meta, _vcf ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_mpileup = BCFTOOLS_MPILEUP.out.index.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    tbi_mpileup = BCFTOOLS_MPILEUP.out.index.branch { meta, _tbi ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more mpileup(s) from the same sample
-    mpileup_samtools = SAMTOOLS_MPILEUP.out.mpileup.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    mpileup_samtools = SAMTOOLS_MPILEUP.out.mpileup.branch { meta, _mpileup ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Merge mpileup and natural order sort them
diff --git a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
index 92570194dd..5c4fd80957 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
@@ -16,7 +16,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
     dict                              // channel: [mandatory]
     dbsnp                             // channel: [optional]
     dbsnp_tbi                         // channel: [optional]
-    dbsnp_vqsr                        // channel: [optional]
     intervals                         // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
     joint_germline                    // boolean: [mandatory] [default: false] joint calling of germline variants
     sentieon_dnascope_emit_mode       // string
@@ -44,7 +43,7 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
             ]
         }
 
-    emit_mode_items = sentieon_dnascope_emit_mode.split(',').each{ it -> it.toLowerCase().trim() }
+    emit_mode_items = sentieon_dnascope_emit_mode.split(',').each{ mode -> mode.toLowerCase().trim() }
     lst = emit_mode_items - 'gvcf'
     emit_vcf = lst.size() > 0 ? lst[0] : ''
 
@@ -52,12 +51,12 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
         cram_intervals_for_sentieon,
         fasta,
         fasta_fai,
-        dbsnp.map{it -> [[:], it]},
-        dbsnp_tbi.map{it -> [[:], it]},
-        sentieon_dnascope_model.map{it -> [[:], it]},
+        dbsnp.map{dbsnp_ -> [[:], dbsnp_]},
+        dbsnp_tbi.map{dbsnp_tbi_ -> [[:], dbsnp_tbi_]},
+        sentieon_dnascope_model.map{sentieon_dnascope_model_ -> [[:], sentieon_dnascope_model_]},
         sentieon_dnascope_pcr_indel_model,
         emit_vcf,
-        emit_mode_items.any{ it.equals('gvcf') })
+        emit_mode_items.any{ mode -> mode.equals('gvcf') })
 
     if (joint_germline) {
         genotype_intervals = SENTIEON_DNASCOPE.out.gvcf
@@ -72,33 +71,33 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
     dnascope_vcf_branch = SENTIEON_DNASCOPE.out.vcf.map{
             meta, vcf_ -> [ meta - meta.subMap('intervals_name'), vcf_]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _vcf ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     dnascope_vcf_tbi_branch = SENTIEON_DNASCOPE.out.vcf_tbi.map{
             meta, vcf_tbi -> [ meta - meta.subMap('intervals_name'), vcf_tbi]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _tbi ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     haplotyper_gvcf_branch = SENTIEON_DNASCOPE.out.gvcf.map{
             meta, gvcf_ -> [ meta - meta.subMap('intervals_name'), gvcf_]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _gvcf ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     haplotyper_gvcf_tbi_branch = SENTIEON_DNASCOPE.out.gvcf_tbi.map{
             meta, gvcf_tbi -> [ meta - meta.subMap('intervals_name'), gvcf_tbi]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _tbi ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     // Per-sample merge. Wrap the (already-`intervals_name`-stripped) meta in
diff --git a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
index a2aba1ade3..f014af1740 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
@@ -16,7 +16,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
     dict                           // channel: [mandatory]
     dbsnp                          // channel: [optional]
     dbsnp_tbi                      // channel: [optional]
-    dbsnp_vqsr                     // channel: [optional]
     intervals                      // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
     joint_germline                 // boolean: [mandatory] [default: false] joint calling of germline variants
     sentieon_haplotyper_emit_mode
@@ -42,7 +41,7 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
             ]
         }
 
-    emit_mode_items = sentieon_haplotyper_emit_mode.split(',').each{ it -> it.toLowerCase().trim() }
+    emit_mode_items = sentieon_haplotyper_emit_mode.split(',').each{ mode -> mode.toLowerCase().trim() }
     lst = emit_mode_items - 'gvcf'
     emit_vcf = lst.size() > 0 ? lst[0] : ''
 
@@ -53,7 +52,7 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
         dbsnp.map{file -> [[id:'dbsnp'], file]},
         dbsnp_tbi.map{file -> [[id:'dbsnp'], file]},
         emit_vcf,
-        emit_mode_items.any{ it.equals('gvcf') })
+        emit_mode_items.any{ mode -> mode.equals('gvcf') })
 
     if (joint_germline) {
         genotype_intervals = SENTIEON_HAPLOTYPER.out.gvcf
@@ -68,33 +67,33 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
     haplotyper_vcf_branch = SENTIEON_HAPLOTYPER.out.vcf.map{
             meta, vcf_ -> [ meta - meta.subMap('intervals_name'), vcf_]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _vcf ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     haplotyper_vcf_tbi_branch = SENTIEON_HAPLOTYPER.out.vcf_tbi.map{
             meta, vcf_tbi -> [ meta - meta.subMap('intervals_name'), vcf_tbi]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _tbi ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     haplotyper_gvcf_branch = SENTIEON_HAPLOTYPER.out.gvcf.map{
             meta, gvcf_ -> [ meta - meta.subMap('intervals_name'), gvcf_]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _gvcf ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     haplotyper_gvcf_tbi_branch = SENTIEON_HAPLOTYPER.out.gvcf_tbi.map{
             meta, gvcf_tbi -> [ meta - meta.subMap('intervals_name'), gvcf_tbi]
         }
-        .branch{
-            intervals:    it[0].num_intervals > 1
-            no_intervals: it[0].num_intervals <= 1
+        .branch{ meta, _tbi ->
+            intervals:    meta.num_intervals > 1
+            no_intervals: meta.num_intervals <= 1
         }
 
     // Per-sample merge. Wrap the (already-`intervals_name`-stripped) meta in
diff --git a/subworkflows/local/bam_variant_calling_single_strelka/main.nf b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
index 6c469651e2..004a57ca2c 100644
--- a/subworkflows/local/bam_variant_calling_single_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
@@ -27,24 +27,24 @@ workflow BAM_VARIANT_CALLING_SINGLE_STRELKA {
     STRELKA_SINGLE(cram_intervals, fasta, fasta_fai)
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    genome_vcf = STRELKA_SINGLE.out.genome_vcf.branch{
+    genome_vcf = STRELKA_SINGLE.out.genome_vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_out = STRELKA_SINGLE.out.vcf.branch{
+    vcf_out = STRELKA_SINGLE.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_out = STRELKA_SINGLE.out.vcf_tbi.branch{
+    tbi_out = STRELKA_SINGLE.out.vcf_tbi.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
index ad101d1590..cea076093f 100644
--- a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
@@ -21,8 +21,8 @@ workflow BAM_VARIANT_CALLING_SINGLE_TIDDIT {
     TABIX_BGZIP_TIDDIT_SV(TIDDIT_SV.out.vcf)
 
     ploidy = TIDDIT_SV.out.ploidy
-    vcf = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, gz, tbi -> [meta + [variantcaller: 'tiddit'], gz] }
-    tbi = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, gz, tbi -> [meta + [variantcaller: 'tiddit'], tbi] }
+    vcf = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, gz, _tbi -> [meta + [variantcaller: 'tiddit'], gz] }
+    tbi = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, _gz, tbi -> [meta + [variantcaller: 'tiddit'], tbi] }
 
     versions = versions.mix(TIDDIT_SV.out.versions)
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index f43549b2f1..e0027be4fc 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -163,8 +163,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             intervals_bed_gz_tbi_combined,
         )
 
-        vcf_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.vcf
-        tbi_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.tbi
+        vcf_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.diploid_sv_vcf.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.somatic_sv_vcf)
+        tbi_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.diploid_sv_vcf_tbi.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.somatic_sv_vcf_tbi)
         versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.versions)
     }
 
@@ -186,7 +186,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
     // STRELKA
     if (tools && tools.split(',').contains('strelka')) {
         cram_strelka = tools.split(',').contains('manta')
-            ? cram.join(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.candidate_small_indels_vcf, failOnDuplicate: true, failOnMismatch: true).join(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.candidate_small_indels_vcf_tbi, failOnDuplicate: true, failOnMismatch: true)
+            // Manta's candidate small indels feed Strelka; strip the manta variantcaller tag so the meta matches cram for the join
+            ? cram.join(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.candidate_small_indels_vcf.map { meta, vcf -> [meta - meta.subMap('variantcaller'), vcf] }, failOnDuplicate: true, failOnMismatch: true).join(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.candidate_small_indels_vcf_tbi.map { meta, tbi -> [meta - meta.subMap('variantcaller'), tbi] }, failOnDuplicate: true, failOnMismatch: true)
             : cram.map { meta, normal_cram, normal_crai, tumor_cram, tumor_crai -> [meta, normal_cram, normal_crai, tumor_cram, tumor_crai, [], []] }
 
         BAM_VARIANT_CALLING_SOMATIC_STRELKA(
diff --git a/subworkflows/local/bam_variant_calling_somatic_controlfreec/main.nf b/subworkflows/local/bam_variant_calling_somatic_controlfreec/main.nf
index 43ef6a74d3..1234ec96f2 100644
--- a/subworkflows/local/bam_variant_calling_somatic_controlfreec/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_controlfreec/main.nf
@@ -29,7 +29,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_CONTROLFREEC {
     //Creates empty channel if file is missing
     cnv_files = FREEC_SOMATIC.out.CNV
     .map{ meta, cnv ->
-        def tumor_file = cnv instanceof List ? cnv.find { it.toString().endsWith("gz_CNVs") } : cnv //only find if its a list, else it returns only the filename without the path
+        def tumor_file = cnv instanceof List ? cnv.find { cnv_file -> cnv_file.toString().endsWith("gz_CNVs") } : cnv //only find if its a list, else it returns only the filename without the path
         if (!tumor_file){
             error("CNVs tumor file not found for sample $meta.id")
         }
@@ -38,7 +38,7 @@ workflow BAM_VARIANT_CALLING_SOMATIC_CONTROLFREEC {
 
     ratio_files = FREEC_SOMATIC.out.ratio
     .map{ meta, ratio ->
-        def tumor_file = ratio instanceof List ? ratio.find { it.toString().endsWith("gz_ratio.txt") } : ratio //same here as cnv
+        def tumor_file = ratio instanceof List ? ratio.find { ratio_file -> ratio_file.toString().endsWith("gz_ratio.txt") } : ratio //same here as cnv
         if (!tumor_file){
             error("Ratio tumor file not found for sample $meta.id")
         }
diff --git a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
index e4ab083fca..f1766192c8 100644
--- a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
@@ -17,35 +17,36 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
     versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
-    cram_intervals = cram.combine(intervals).map{ it ->
-        def bed_gz = it.size() > 5 ? it[5] : []
-        def bed_tbi = it.size() > 5 ? it[6] : []
+    cram_intervals = cram.combine(intervals).map{ combined ->
+        def bed_gz = combined.size() > 5 ? combined[5] : []
+        def bed_tbi = combined.size() > 5 ? combined[6] : []
 
-        [it[0], it[1], it[2], it[3], it[4], bed_gz, bed_tbi]
+        [combined[0], combined[1], combined[2], combined[3], combined[4], bed_gz, bed_tbi]
     }
 
     MANTA_SOMATIC(cram_intervals, fasta, fasta_fai, [])
 
-    candidate_small_indels_vcf = MANTA_SOMATIC.out.candidate_small_indels_vcf
-    candidate_small_indels_vcf_tbi = MANTA_SOMATIC.out.candidate_small_indels_vcf_tbi
-    candidate_sv_vcf = MANTA_SOMATIC.out.candidate_sv_vcf
-    diploid_sv_vcf = MANTA_SOMATIC.out.diploid_sv_vcf
-    diploid_sv_vcf_tbi = MANTA_SOMATIC.out.diploid_sv_vcf_tbi
-    somatic_sv_vcf = MANTA_SOMATIC.out.somatic_sv_vcf
-    somatic_sv_vcf_tbi = MANTA_SOMATIC.out.somatic_sv_vcf_tbi
-
-    // Only diploid and somatic SV should get annotated
     // add variantcaller to meta map
-    vcf = channel.empty().mix(diploid_sv_vcf, somatic_sv_vcf).map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
-    tbi = channel.empty().mix(diploid_sv_vcf_tbi, somatic_sv_vcf_tbi).map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_small_indels_vcf = MANTA_SOMATIC.out.candidate_small_indels_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_small_indels_vcf_tbi = MANTA_SOMATIC.out.candidate_small_indels_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_sv_vcf = MANTA_SOMATIC.out.candidate_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_sv_vcf_tbi = MANTA_SOMATIC.out.candidate_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    diploid_sv_vcf = MANTA_SOMATIC.out.diploid_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    diploid_sv_vcf_tbi = MANTA_SOMATIC.out.diploid_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    somatic_sv_vcf = MANTA_SOMATIC.out.somatic_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    somatic_sv_vcf_tbi = MANTA_SOMATIC.out.somatic_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
     versions = versions.mix(MANTA_SOMATIC.out.versions)
 
     emit:
     candidate_small_indels_vcf
     candidate_small_indels_vcf_tbi
-    vcf
-    tbi
+    candidate_sv_vcf
+    candidate_sv_vcf_tbi
+    diploid_sv_vcf
+    diploid_sv_vcf_tbi
+    somatic_sv_vcf
+    somatic_sv_vcf_tbi
 
     versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
index e8e9dea649..8b65207486 100644
--- a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
@@ -32,8 +32,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
     // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
-    germline_resource_pileup = germline_resource.filter { it != [] }
-    germline_resource_pileup_tbi = germline_resource_tbi.filter { it != [] }
+    germline_resource_pileup = germline_resource.filter { germline_resource_ -> germline_resource_ != [] }
+    germline_resource_pileup_tbi = germline_resource_tbi.filter { germline_resource_tbi_ -> germline_resource_tbi_ != [] }
 
     // Combine input and intervals for spread and gather strategy
     //   Move num_intervals to meta map and reorganize channel for MUTECT2_PAIRED module
@@ -68,27 +68,27 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_branch = MUTECT2_PAIRED.out.vcf.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    vcf_branch = MUTECT2_PAIRED.out.vcf.branch { meta, _vcf ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_branch = MUTECT2_PAIRED.out.tbi.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    tbi_branch = MUTECT2_PAIRED.out.tbi.branch { meta, _tbi ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    stats_branch = MUTECT2_PAIRED.out.stats.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    stats_branch = MUTECT2_PAIRED.out.stats.branch { meta, _stats ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    f1r2_branch = MUTECT2_PAIRED.out.f1r2.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    f1r2_branch = MUTECT2_PAIRED.out.f1r2.branch { meta, _f1r2 ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -141,15 +141,15 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     GETPILEUPSUMMARIES_TUMOR(pileup_tumor, fasta, fai, dict, germline_resource_pileup, germline_resource_pileup_tbi)
 
     // Figuring out if there is one or more table(s) from the same sample
-    pileup_table_normal_branch = GETPILEUPSUMMARIES_NORMAL.out.table.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    pileup_table_normal_branch = GETPILEUPSUMMARIES_NORMAL.out.table.branch { meta, _table ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more table(s) from the same sample
-    pileup_table_tumor_branch = GETPILEUPSUMMARIES_TUMOR.out.table.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    pileup_table_tumor_branch = GETPILEUPSUMMARIES_TUMOR.out.table.branch { meta, _table ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -220,12 +220,12 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     vcf_mutect2 = FILTERMUTECTCALLS.out.vcf
         .map { meta, vcf_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], vcf_] }
         .concat(vcf.map { meta, vcf_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], vcf_] })
-        .unique { it[0] }
+        .unique { meta, _vcf -> meta }
 
     tbi_mutect2 = FILTERMUTECTCALLS.out.tbi
         .map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] }
         .concat(tbi.map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] })
-        .unique { it[0] }
+        .unique { meta, _tbi -> meta }
 
     versions = versions.mix(CALCULATECONTAMINATION.out.versions)
     versions = versions.mix(FILTERMUTECTCALLS.out.versions)
diff --git a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
index eb960e5131..ae73178155 100644
--- a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
@@ -27,17 +27,17 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
     STRELKA_SOMATIC(cram_intervals, fasta, fasta_fai )
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_indels = STRELKA_SOMATIC.out.vcf_indels.branch{
+    vcf_indels = STRELKA_SOMATIC.out.vcf_indels.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_snvs = STRELKA_SOMATIC.out.vcf_snvs.branch{
+    vcf_snvs = STRELKA_SOMATIC.out.vcf_snvs.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -48,17 +48,17 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
     MERGE_STRELKA_SNVS(vcf_snvs_to_merge, dict)
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_indels = STRELKA_SOMATIC.out.vcf_indels_tbi.branch{
+    tbi_indels = STRELKA_SOMATIC.out.vcf_indels_tbi.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_snvs = STRELKA_SOMATIC.out.vcf_snvs_tbi.branch{
+    tbi_snvs = STRELKA_SOMATIC.out.vcf_snvs_tbi.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Mix intervals and no_intervals channels together
diff --git a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
index 8f0e083a59..c66b1b9148 100644
--- a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
@@ -38,16 +38,16 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TNSCOPE {
         [[],[]] // cosmic_tbi
     )
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_branch = SENTIEON_TNSCOPE.out.vcf.branch{
+    vcf_branch = SENTIEON_TNSCOPE.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_branch = SENTIEON_TNSCOPE.out.index.branch{
+    tbi_branch = SENTIEON_TNSCOPE.out.index.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     vcf_to_merge = vcf_branch.intervals.map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ] }.groupTuple()
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 1a478957b1..e878d4dff9 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -31,7 +31,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
     germline_resource             // channel: [optional]  germline_resource
     germline_resource_tbi         // channel: [optional]  germline_resource_tbi
     intervals                     // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
-    intervals_bed_gz_tbi          // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ] or [ [], [], 0 ] if no intervals
     intervals_bed_combined        // channel: [mandatory] intervals/target regions in one file unzipped
     intervals_bed_gz_tbi_combined // channel: [mandatory] intervals/target regions in one file zipped
     mappability
@@ -102,7 +101,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
             fasta,
             fasta_fai,
             [[id: "null"], []],
-            cnvkit_reference.map { it -> [[id: it[0].baseName], it] },
+            cnvkit_reference.map { reference -> [[id: reference[0].baseName], reference] },
         )
 
         versions = versions.mix(BAM_VARIANT_CALLING_CNVKIT.out.versions)
@@ -181,8 +180,8 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
             intervals_bed_gz_tbi_combined,
         )
 
-        vcf_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.vcf
-        tbi_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.tbi
+        vcf_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.tumor_sv_vcf
+        tbi_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.tumor_sv_vcf_tbi
         versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.versions)
     }
 
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
index 702d6361c8..fd38480bbc 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
@@ -20,17 +20,17 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ {
     LOFREQ(input_intervals, fasta, fai) // Call variants with LoFreq
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_branch = LOFREQ.out.vcf.branch{
+    vcf_branch = LOFREQ.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_branch = LOFREQ.out.tbi.branch{
+    tbi_branch = LOFREQ.out.tbi.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
index 9fc89e931c..380121ef9b 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
@@ -18,30 +18,32 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA {
     versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
-    cram_intervals = cram.combine(intervals).map{ it ->
-        def bed_gz = it.size() > 3 ? it[3] : []
-        def bed_tbi = it.size() > 3 ? it[4] : []
+    cram_intervals = cram.combine(intervals).map{ combined ->
+        def bed_gz = combined.size() > 3 ? combined[3] : []
+        def bed_tbi = combined.size() > 3 ? combined[4] : []
 
-        [it[0], it[1], it[2], bed_gz, bed_tbi]
+        [combined[0], combined[1], combined[2], bed_gz, bed_tbi]
     }
 
     MANTA_TUMORONLY(cram_intervals, fasta, fasta_fai, [])
 
-    small_indels_vcf = MANTA_TUMORONLY.out.candidate_small_indels_vcf
-    candidate_sv_vcf = MANTA_TUMORONLY.out.candidate_sv_vcf
-    tumor_sv_vcf = MANTA_TUMORONLY.out.tumor_sv_vcf
-    tumor_sv_vcf_tbi = MANTA_TUMORONLY.out.tumor_sv_vcf_tbi
-
-    // Only tumor sv should get annotated
     // add variantcaller to meta map
-    vcf = tumor_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
-    tbi = tumor_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_small_indels_vcf = MANTA_TUMORONLY.out.candidate_small_indels_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_small_indels_vcf_tbi = MANTA_TUMORONLY.out.candidate_small_indels_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    candidate_sv_vcf = MANTA_TUMORONLY.out.candidate_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    candidate_sv_vcf_tbi = MANTA_TUMORONLY.out.candidate_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
+    tumor_sv_vcf = MANTA_TUMORONLY.out.tumor_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
+    tumor_sv_vcf_tbi = MANTA_TUMORONLY.out.tumor_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
     versions = versions.mix(MANTA_TUMORONLY.out.versions)
 
     emit:
-    vcf
-    tbi
+    candidate_small_indels_vcf
+    candidate_small_indels_vcf_tbi
+    candidate_sv_vcf
+    candidate_sv_vcf_tbi
+    tumor_sv_vcf
+    tumor_sv_vcf_tbi
 
     versions
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
index bfd4c278ac..b895920247 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
@@ -31,8 +31,8 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
     // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
-    germline_resource_pileup = germline_resource.filter { it != [] }
-    germline_resource_pileup_tbi = germline_resource_tbi.filter { it != [] }
+    germline_resource_pileup = germline_resource.filter { germline_resource_ -> germline_resource_ != [] }
+    germline_resource_pileup_tbi = germline_resource_tbi.filter { germline_resource_tbi_ -> germline_resource_tbi_ != [] }
 
     // Combine input and intervals for spread and gather strategy
     input_intervals = input
@@ -58,27 +58,27 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_branch = MUTECT2.out.vcf.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    vcf_branch = MUTECT2.out.vcf.branch { meta, _vcf ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_branch = MUTECT2.out.tbi.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    tbi_branch = MUTECT2.out.tbi.branch { meta, _tbi ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more stats(s) from the same sample
-    stats_branch = MUTECT2.out.stats.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    stats_branch = MUTECT2.out.stats.branch { meta, _stats ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Figuring out if there is one or more f1r2(s) from the same sample
-    f1r2_branch = MUTECT2.out.f1r2.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    f1r2_branch = MUTECT2.out.f1r2.branch { meta, _f1r2 ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -105,9 +105,9 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     GETPILEUPSUMMARIES(pileup_input, fasta, fai, dict, germline_resource_pileup, germline_resource_pileup_tbi)
 
     // Figuring out if there is one or more table(s) from the same sample
-    pileup_table_branch = GETPILEUPSUMMARIES.out.table.branch {
-        intervals: it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+    pileup_table_branch = GETPILEUPSUMMARIES.out.table.branch { meta, _table ->
+        intervals: meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     // Only when using intervals
@@ -157,12 +157,12 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     vcf_mutect2 = FILTERMUTECTCALLS.out.vcf
         .map { meta, vcf_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], vcf_] }
         .concat(vcf.map { meta, vcf_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], vcf_] })
-        .unique { it[0] }
+        .unique { meta, _vcf -> meta }
 
     tbi_mutect2 = FILTERMUTECTCALLS.out.tbi
         .map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] }
         .concat(tbi.map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] })
-        .unique { it[0] }
+        .unique { meta, _tbi -> meta }
 
     versions = versions.mix(CALCULATECONTAMINATION.out.versions)
     versions = versions.mix(FILTERMUTECTCALLS.out.versions)
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
index 91ec26a72b..0c233bad0e 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
@@ -38,16 +38,16 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE {
         [[],[]] // cosmic_tbi
     )
     // Figuring out if there is one or more vcf(s) from the same sample
-    vcf_branch = SENTIEON_TNSCOPE.out.vcf.branch{
+    vcf_branch = SENTIEON_TNSCOPE.out.vcf.branch{ meta, _vcf ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
     // Figuring out if there is one or more tbi(s) from the same sample
-    tbi_branch = SENTIEON_TNSCOPE.out.index.branch{
+    tbi_branch = SENTIEON_TNSCOPE.out.index.branch{ meta, _tbi ->
         // Use meta.num_intervals to asses number of intervals
-        intervals:    it[0].num_intervals > 1
-        no_intervals: it[0].num_intervals <= 1
+        intervals:    meta.num_intervals > 1
+        no_intervals: meta.num_intervals <= 1
     }
 
     vcf_to_merge = vcf_branch.intervals.map{ meta, vcf -> [ groupKey(meta, meta.num_intervals), vcf ] }.groupTuple()
diff --git a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
index 02985a7939..15b1e97c4d 100644
--- a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
+++ b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
@@ -20,7 +20,7 @@ workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
     // Reports run on cram
     SAMTOOLS_STATS(cram, fasta)
 
-    MOSDEPTH(cram.combine(intervals.map { meta, bed -> [bed ?: []] }), fasta)
+    MOSDEPTH(cram.combine(intervals.map { _meta, bed -> [bed ?: []] }), fasta)
 
     // Gather all reports generated
     reports = reports.mix(SAMTOOLS_STATS.out.stats)
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index d05b1f980b..3aa61c69fc 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -96,7 +96,7 @@ workflow PREPARE_GENOME {
         versions = versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
     }
     else if (dict_in) {
-        dict = channel.fromPath(dict_in).map { it -> [[id: 'dict'], it] }.collect()
+        dict = channel.fromPath(dict_in).map { dict_ -> [[id: 'dict'], dict_] }.collect()
     }
     else {
         dict = channel.empty()
@@ -108,7 +108,7 @@ workflow PREPARE_GENOME {
         versions = versions.mix(SAMTOOLS_FAIDX.out.versions)
     }
     else if (fasta_fai_in) {
-        fasta_fai = channel.fromPath(fasta_fai_in).map { it -> [[id: 'fai'], it] }.collect()
+        fasta_fai = channel.fromPath(fasta_fai_in).map { fai -> [[id: 'fai'], fai] }.collect()
     }
     else {
         fasta_fai = channel.empty()
@@ -133,8 +133,8 @@ workflow PREPARE_GENOME {
                 .splitCsv(header: false, sep: ',')
                 .flatMap { id, fafile -> [['id', id], ['fasta', file(fafile, checkIfExists: true)]] }
                 .groupTuple()
-                .map { it -> it[1] }
-                .collect { [it] }
+                .map { group -> group[1] }
+                .collect { fasta_group -> [fasta_group] }
 
             bbsplit_index = BBMAP_INDEX(
                 [[id: "build_index"], []],
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index 581612e465..f6899250d0 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -33,9 +33,9 @@ workflow PREPARE_INTERVALS {
         file("${outdir}/no_intervals.bed.gz").text     = "no_intervals\n"
         file("${outdir}/no_intervals.bed.gz.tbi").text = "no_intervals\n"
 
-        intervals_bed        = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ it, 0 ] }
-        intervals_bed_gz_tbi = channel.fromPath(file("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ it -> [ it, 0 ] }
-        intervals_combined   = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ it -> [ [ id:it.simpleName ], it ] }
+        intervals_bed        = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ bed -> [ bed, 0 ] }
+        intervals_bed_gz_tbi = channel.fromPath(file("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ files -> [ files, 0 ] }
+        intervals_combined   = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ bed -> [ [ id:bed.simpleName ], bed ] }
     } else if (step != 'annotate' && step != 'controlfreec') {
         // If no interval/target file is provided, then generated intervals from FASTA file
         if (!intervals) {
@@ -43,14 +43,14 @@ workflow PREPARE_INTERVALS {
 
             intervals_combined = BUILD_INTERVALS.out.output
 
-            CREATE_INTERVALS_BED(intervals_combined.map{ meta, path -> path }, nucleotides_per_second)
+            CREATE_INTERVALS_BED(intervals_combined.map{ _meta, path -> path }, nucleotides_per_second)
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
 
             versions = versions.mix(BUILD_INTERVALS.out.versions)
             versions = versions.mix(CREATE_INTERVALS_BED.out.versions)
         } else {
-            intervals_combined = channel.fromPath(file(intervals)).map{it -> [ [ id:it.baseName ], it ] }
+            intervals_combined = channel.fromPath(file(intervals)).map{bed -> [ [ id:bed.baseName ], bed ] }
             CREATE_INTERVALS_BED(file(intervals), nucleotides_per_second)
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
@@ -84,24 +84,24 @@ workflow PREPARE_INTERVALS {
                 }
                 [ duration, intervalFile ]
             }.toSortedList({ a, b -> b[0] <=> a[0] })
-            .flatten().collate(2).map{ duration, intervalFile -> intervalFile }.collect()
+            .flatten().collate(2).map{ _duration, intervalFile -> intervalFile }.collect()
             // Adding number of intervals as elements
-            .map{ it -> [ it, it.size() ] }
+            .map{ files -> [ files, files.size() ] }
             .transpose()
 
         // 2. Create bed.gz and bed.gz.tbi for each interval file. They are split by region (see above)
-        TABIX_BGZIPTABIX_INTERVAL_SPLIT(intervals_bed.map{ file, num_intervals -> [ [ id:file.baseName], file ] })
+        TABIX_BGZIPTABIX_INTERVAL_SPLIT(intervals_bed.map{ file, _num_intervals -> [ [ id:file.baseName], file ] })
 
-        intervals_bed_gz_tbi = TABIX_BGZIPTABIX_INTERVAL_SPLIT.out.gz_index.map{ meta, bed, tbi -> [ bed, tbi ] }.toList()
+        intervals_bed_gz_tbi = TABIX_BGZIPTABIX_INTERVAL_SPLIT.out.gz_index.map{ _meta, bed, tbi -> [ bed, tbi ] }.toList()
             // Adding number of intervals as elements
-            .map{ it -> [ it, it.size() ] }
+            .map{ files -> [ files, files.size() ] }
             .transpose()
     }
 
     TABIX_BGZIPTABIX_INTERVAL_COMBINED(intervals_combined)
 
-    intervals_bed_combined        = intervals_combined.map{meta, bed -> bed }.collect()
-    intervals_bed_gz_tbi_combined = TABIX_BGZIPTABIX_INTERVAL_COMBINED.out.gz_index.map{meta, gz, tbi -> [gz, tbi] }.collect()
+    intervals_bed_combined        = intervals_combined.map{_meta, bed -> bed }.collect()
+    intervals_bed_gz_tbi_combined = TABIX_BGZIPTABIX_INTERVAL_COMBINED.out.gz_index.map{_meta, gz, tbi -> [gz, tbi] }.collect()
 
     emit:
     // Intervals split for parallel execution
diff --git a/subworkflows/local/prepare_reference_cnvkit/main.nf b/subworkflows/local/prepare_reference_cnvkit/main.nf
index 9eaca9eff7..74b8b3694c 100644
--- a/subworkflows/local/prepare_reference_cnvkit/main.nf
+++ b/subworkflows/local/prepare_reference_cnvkit/main.nf
@@ -10,7 +10,7 @@ workflow PREPARE_REFERENCE_CNVKIT {
     versions = channel.empty()
 
     // prepare a antitarget reference files for tumor_only mode of cnvkit
-    CNVKIT_ANTITARGET(intervals_bed_combined.flatten().map { it -> [[id: 'intervals'], it] })
+    CNVKIT_ANTITARGET(intervals_bed_combined.flatten().map { bed -> [[id: 'intervals'], bed] })
     CNVKIT_REFERENCE(fasta.map { _meta, fasta_ -> [fasta_] }, intervals_bed_combined, CNVKIT_ANTITARGET.out.bed.map { _meta, bed -> [bed] })
 
     versions = versions.mix(CNVKIT_ANTITARGET.out.versions)
diff --git a/subworkflows/local/prepare_snpsift_databases/main.nf b/subworkflows/local/prepare_snpsift_databases/main.nf
index e524212d72..30ac7c0373 100644
--- a/subworkflows/local/prepare_snpsift_databases/main.nf
+++ b/subworkflows/local/prepare_snpsift_databases/main.nf
@@ -12,26 +12,26 @@ workflow PREPARE_SNPSIFT_DATABASES {
     ch_configs = channel.fromList(val_db_configs)
 
     // Branch: create vardb if not provided
-    ch_branched = ch_configs.branch {
-        has_vardb: it.vardb != null
+    ch_branched = ch_configs.branch { config ->
+        has_vardb: config.vardb != null
         needs_vardb: true
     }
 
     // Create vardbs for databases that need them
     // Convert semicolon-separated fields to comma-separated (SnpSift expects commas)
     SNPSIFT_ANNMEMCREATE(
-        ch_branched.needs_vardb.map { [[id: it.vcf.baseName], it.vcf, it.tbi, it.fields ? it.fields.replace(';', ',') : ''] }
+        ch_branched.needs_vardb.map { config -> [[id: config.vcf.baseName], config.vcf, config.tbi, config.fields ? config.fields.replace(';', ',') : ''] }
     )
 
     // Join created vardbs back with their configs
     ch_created = SNPSIFT_ANNMEMCREATE.out.database
         .map { meta, vardb -> [meta.id, vardb] }
-        .join(ch_branched.needs_vardb.map { [it.vcf.baseName, it] })
+        .join(ch_branched.needs_vardb.map { config -> [config.vcf.baseName, config] })
         .map { _id, vardb, config -> [config.vcf, config.tbi, vardb, config.fields ? config.fields.replace(';', ',') : '', config.prefix ?: ''] }
 
     // Configs with pre-built vardb
     ch_prebuilt = ch_branched.has_vardb
-        .map { [it.vcf, it.tbi, it.vardb, it.fields ? it.fields.replace(';', ',') : '', it.prefix ?: ''] }
+        .map { config -> [config.vcf, config.tbi, config.vardb, config.fields ? config.fields.replace(';', ',') : '', config.prefix ?: ''] }
 
     // Collect all into output tuple
     ch_db_tuple = ch_prebuilt
@@ -39,11 +39,11 @@ workflow PREPARE_SNPSIFT_DATABASES {
         .toList()
         .map { list ->
             [
-                list.collect { it[0] },  // db_vcf
-                list.collect { it[1] },  // db_vcf_tbi
-                list.collect { it[2] },  // db_vardb
-                list.collect { it[3] },  // db_fields
-                list.collect { it[4] }   // db_prefixes
+                list.collect { row -> row[0] },  // db_vcf
+                list.collect { row -> row[1] },  // db_vcf_tbi
+                list.collect { row -> row[2] },  // db_vardb
+                list.collect { row -> row[3] },  // db_fields
+                list.collect { row -> row[4] }   // db_prefixes
             ]
         }
 
diff --git a/subworkflows/local/samplesheet_to_channel/main.nf b/subworkflows/local/samplesheet_to_channel/main.nf
index 6bbde0f514..92e76472be 100644
--- a/subworkflows/local/samplesheet_to_channel/main.nf
+++ b/subworkflows/local/samplesheet_to_channel/main.nf
@@ -10,7 +10,6 @@ workflow SAMPLESHEET_TO_CHANNEL {
     ascat_loci_rt                 // Path: ascat loci rt
     bcftools_annotations          // Path: bcftools annotations
     bcftools_annotations_tbi      // Path: bcftools annotations tbi
-    bcftools_columns              // Path: bcftools columns
     bcftools_header_lines         // Path: bcftools header lines
     build_only_index              // Boolean: build only index
     dbsnp                         // Path: dbsnp
@@ -50,8 +49,8 @@ workflow SAMPLESHEET_TO_CHANNEL {
         .groupTuple()
         .map { patient, samples ->
             // Count samples with status 0 and status 1
-            def status0_count = samples.count { it.status == 0 }
-            def status1_count = samples.count { it.status == 1 }
+            def status0_count = samples.count { sample -> sample.status == 0 }
+            def status1_count = samples.count { sample -> sample.status == 1 }
 
             // Check the condition and exit with an error if met
             if (status1_count == 1 && status0_count > 1) {
@@ -67,7 +66,7 @@ workflow SAMPLESHEET_TO_CHANNEL {
             [combination_key, [meta.patient, meta.sample, meta.status, meta.lane]]
         }
         .groupTuple()
-        .map { combination_key, combination_list ->
+        .map { _combination_key, combination_list ->
             if (combination_list.size() > 1) {
                 def patient = combination_list[0][0]
                 def sample = combination_list[0][1]
@@ -87,7 +86,7 @@ workflow SAMPLESHEET_TO_CHANNEL {
         .groupTuple()
         .map { patient, samples ->
             // Return the patient and the list of sample ids
-            [patient, samples.collect { it.sample }]
+            [patient, samples.collect { sample -> sample.sample }]
         }
         // Flatten to [sample_id, patient] pairs
         .flatMap { patient, sample_ids -> sample_ids.collect { sample_id -> [sample_id, patient] } }
@@ -242,7 +241,7 @@ workflow SAMPLESHEET_TO_CHANNEL {
         // 1. the sample-sheet only contains normal-samples, but some of the requested tools require tumor-samples, and
         // 2. the sample-sheet only contains tumor-samples, but some of the requested tools require normal-samples.
         input_sample
-            .filter { it[0].status == 1 }
+            .filter { sample -> sample[0].status == 1 }
             .ifEmpty {
                 // In this case, the sample-sheet contains no tumor-samples
                 if (!build_only_index) {
@@ -260,7 +259,7 @@ workflow SAMPLESHEET_TO_CHANNEL {
             }
 
         input_sample
-            .filter { it[0].status == 0 }
+            .filter { sample -> sample[0].status == 0 }
             .ifEmpty {
                 // In this case, the sample-sheet contains no normal/germline-samples
                 def tools_requiring_normal_samples = ['ascat', 'deepvariant', 'haplotypecaller', 'msisensorpro']
@@ -433,8 +432,8 @@ workflow SAMPLESHEET_TO_CHANNEL {
 
     // Fails when missing sex information for CNV tools or varlociraptor
     if (tools && (tools.split(',').contains('ascat') || tools.split(',').contains('controlfreec') || tools.split(',').contains('varlociraptor'))) {
-        input_sample.map {
-            if (it[0].sex == 'NA') {
+        input_sample.map { sample ->
+            if (sample[0].sex == 'NA') {
                 error("Please specify sex information for each sample in your samplesheet when using '--tools' with 'ascat' or 'controlfreec' or 'varlociraptor'.\nhttps://nf-co.re/sarek/usage#input-samplesheet-configurations")
             }
         }
@@ -442,8 +441,8 @@ workflow SAMPLESHEET_TO_CHANNEL {
 
     // Fails when varlociraptor is enable for tumor samples but no contamination is provided
     if (tools && tools.split(',').contains('varlociraptor')) {
-        input_sample.map {
-            if (it[0].status == 1 && !it[0].containsKey('contamination')) {
+        input_sample.map { sample ->
+            if (sample[0].status == 1 && !sample[0].containsKey('contamination')) {
                 error("Please specify contamination information for each tumor sample in your samplesheet when using '--tools' with 'varlociraptor'.\nhttps://nf-co.re/sarek/usage#input-samplesheet-configurations")
             }
         }
diff --git a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
index 0aa70f656c..0142d6e3bd 100644
--- a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
@@ -160,7 +160,6 @@ workflow PIPELINE_INITIALISATION {
         params.ascat_loci_rt,
         params.bcftools_annotations,
         params.bcftools_annotations_tbi,
-        params.bcftools_columns,
         params.bcftools_header_lines,
         params.build_only_index,
         params.dbsnp,
diff --git a/subworkflows/local/vcf_consensus/main.nf b/subworkflows/local/vcf_consensus/main.nf
index 291d360684..8cedb412b2 100644
--- a/subworkflows/local/vcf_consensus/main.nf
+++ b/subworkflows/local/vcf_consensus/main.nf
@@ -15,7 +15,7 @@ workflow CONSENSUS {
     ch_versions = channel.empty()
 
     ch_vcfs = vcfs
-        .branch{ meta, vcf, tbi ->
+        .branch{ meta, _vcf, _tbi ->
             // Somatic Strelka samples have tumor_id field (tumor-normal pairs)
             // This is semantically equivalent to checking status == '1' (tumor) but more explicit
             strelka_somatic: meta.variantcaller == 'strelka' && meta.tumor_id
@@ -50,8 +50,8 @@ workflow CONSENSUS {
                             // Sort by vcf name for predictable isec input order
                             // callers list will match isec output order in sites.txt
                             def sorted_pairs = vcf_caller_pairs.sort { a, b -> a[0].name <=> b[0].name }
-                            def sorted_vcfs = sorted_pairs.collect { it[0] }
-                            def callers = sorted_pairs.collect { it[1] }
+                            def sorted_vcfs = sorted_pairs.collect { pair -> pair[0] }
+                            def callers = sorted_pairs.collect { pair -> pair[1] }
                             // file_list, targets_file, regions_file are unused: VCFs are passed positionally
                             // and consensus is computed genome-wide (no region/target restriction)
                             [meta + [callers: callers], sorted_vcfs, tbis, [], [], []]
@@ -62,7 +62,7 @@ workflow CONSENSUS {
 
     // Filter out empty isec results (no consensus variants found)
     ch_isec_with_results = BCFTOOLS_ISEC.out.results
-        .filter { meta, dir ->
+        .filter { _meta, dir ->
             def sites_file = dir.resolve('sites.txt')
             sites_file.exists() && sites_file.size() > 0
         }
diff --git a/subworkflows/local/vcf_varlociraptor_somatic/main.nf b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
index 292c18b340..7234a5d072 100644
--- a/subworkflows/local/vcf_varlociraptor_somatic/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
@@ -62,9 +62,9 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     ch_somatic_vcf_tbi = ch_somatic_vcf.join(TABIX_SOMATIC.out.tbi, by: [0])
 
     // CONCAT SNV / INDEL VCFs COMING FROM STRELKA
-    ch_somatic_branched = ch_somatic_vcf_tbi.branch { items ->
-        strelka: items[0].variantcaller == 'strelka'
-        other: items[0].variantcaller != 'strelka'
+    ch_somatic_branched = ch_somatic_vcf_tbi.branch { meta, _vcf, _tbi ->
+        strelka: meta.variantcaller == 'strelka'
+        other: meta.variantcaller != 'strelka'
     }
 
     // Group somatic strelka SNVs and INDELs by sample for concatenation
@@ -100,9 +100,9 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     def matching_pairs = somatic_with_key.join(germline_with_key, failOnMismatch: false)
 
     // Branch based on whether a matching germline VCF was found
-    def branched = matching_pairs.branch { items ->
-        matched: items.size() == 7
-        unmatched: items.size() == 4
+    def branched = matching_pairs.branch { pair ->
+        matched: pair.size() == 7
+        unmatched: pair.size() == 4
     }
 
     MERGE_GERMLINE_SOMATIC_VCFS(
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index d5c4f474d6..7a0c71fea2 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -23,8 +23,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
@@ -665,8 +664,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
diff --git a/tests/variant_calling_cnvkit.nf.test.snap b/tests/variant_calling_cnvkit.nf.test.snap
index 057cff2288..e37886a5b3 100644
--- a/tests/variant_calling_cnvkit.nf.test.snap
+++ b/tests/variant_calling_cnvkit.nf.test.snap
@@ -7,8 +7,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
@@ -134,19 +133,19 @@
                 "variant_calling/cnvkit/sample4_vs_sample3/multi_intervals.antitarget.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/multi_intervals.target.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/reference.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-diagram.pdf",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-scatter.png",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.bintest.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cnr",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.genemetrics.tsv",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.somatic.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf"
+                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.targetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-diagram.pdf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-scatter.png",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.bintest.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cnr",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.genemetrics.tsv",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.somatic.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.targetcoverage.cnn"
             ],
             [
                 "mosdepth-coverage-per-contig-single.txt:md5,336d786b273c4d4e714d51c44207ff56",
@@ -180,16 +179,16 @@
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f9e727147a7cbca46c2b9b2de3ecae91",
                 "reference.cnn:md5,e0aefc1c8a40e6919dc9cfb686e51f7c",
-                "sample3.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample3.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
-                "sample4.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample4.bintest.cns:md5,b5ccf48e99562082026e94f8d8aa1133",
-                "sample4.call.cns:md5,8202ec610e834c58bb7199761fccd9e5",
-                "sample4.cnr:md5,47d799d37af7ca8022fcac0ec705a3b8",
-                "sample4.cns:md5,50352818fd705424a7ab770d7707c312",
-                "sample4.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
-                "sample4.somatic.call.cns:md5,1cb4febd5c88d306277f3c4c3117d68c",
-                "sample4.targetcoverage.cnn:md5,62c2d1f8765618c454bf4455c2298344"
+                "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
+                "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test2.paired_end.recalibrated.sorted.bintest.cns:md5,b5ccf48e99562082026e94f8d8aa1133",
+                "test2.paired_end.recalibrated.sorted.call.cns:md5,8202ec610e834c58bb7199761fccd9e5",
+                "test2.paired_end.recalibrated.sorted.cnr:md5,47d799d37af7ca8022fcac0ec705a3b8",
+                "test2.paired_end.recalibrated.sorted.cns:md5,50352818fd705424a7ab770d7707c312",
+                "test2.paired_end.recalibrated.sorted.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
+                "test2.paired_end.recalibrated.sorted.somatic.call.cns:md5,1cb4febd5c88d306277f3c4c3117d68c",
+                "test2.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,62c2d1f8765618c454bf4455c2298344"
             ],
             "No BAM files",
             "No CRAM files",
@@ -201,9 +200,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:16:22.938969536"
+        "timestamp": "2026-07-20T10:09:45.402105"
     },
     "-profile test --tools cnvkit --input recalibrated.csv --only_paired_variant_calling": {
         "content": [
@@ -213,8 +212,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
@@ -355,34 +353,34 @@
                 "variant_calling/cnvkit/sample2",
                 "variant_calling/cnvkit/sample2/cnvkit.reference.antitarget-tmp.bed",
                 "variant_calling/cnvkit/sample2/cnvkit.reference.target-tmp.bed",
-                "variant_calling/cnvkit/sample2/sample2-diagram.pdf",
-                "variant_calling/cnvkit/sample2/sample2-scatter.png",
-                "variant_calling/cnvkit/sample2/sample2.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample2/sample2.bintest.cns",
-                "variant_calling/cnvkit/sample2/sample2.call.cns",
-                "variant_calling/cnvkit/sample2/sample2.cnr",
-                "variant_calling/cnvkit/sample2/sample2.cns",
                 "variant_calling/cnvkit/sample2/sample2.cnvcall.vcf",
-                "variant_calling/cnvkit/sample2/sample2.genemetrics.tsv",
-                "variant_calling/cnvkit/sample2/sample2.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample2/sample2.tumor_only.call.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted-diagram.pdf",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted-scatter.png",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.bintest.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.call.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.cnr",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.genemetrics.tsv",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.targetcoverage.cnn",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.tumor_only.call.cns",
                 "variant_calling/cnvkit/sample4_vs_sample3",
                 "variant_calling/cnvkit/sample4_vs_sample3/multi_intervals.antitarget.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/multi_intervals.target.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/reference.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-diagram.pdf",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-scatter.png",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.bintest.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cnr",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.genemetrics.tsv",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.somatic.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf"
+                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.targetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-diagram.pdf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-scatter.png",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.bintest.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cnr",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.genemetrics.tsv",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.somatic.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.targetcoverage.cnn"
             ],
             [
                 "mosdepth-coverage-per-contig-single.txt:md5,73ef9a077df1887f9021a581fbf207bc",
@@ -425,27 +423,27 @@
                 "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,4153756b30abc934f10717c023bd262f",
                 "cnvkit.reference.antitarget-tmp.bed:md5,3d4d20f9f23b39970865d29ef239d20b",
                 "cnvkit.reference.target-tmp.bed:md5,657b25dbda8516624efa8cb2cf3716ca",
-                "sample2.antitargetcoverage.cnn:md5,067115082c4af4b64d58c0dc3a3642e4",
-                "sample2.bintest.cns:md5,7a66b5f63acb05e6dfb0784c215851ec",
-                "sample2.call.cns:md5,f7caeca04aba28b125ce26b511f42afb",
-                "sample2.cnr:md5,d9bdb71ce807051369577ee7f807a40c",
-                "sample2.cns:md5,2b56aac606ba6183d018b30ca58afcec",
-                "sample2.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
-                "sample2.targetcoverage.cnn:md5,e6d0190c1c37ce6e41f76ca5b24ccca3",
-                "sample2.tumor_only.call.cns:md5,70a308c6db7acf1a5fd623936cac6412",
+                "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,067115082c4af4b64d58c0dc3a3642e4",
+                "test2.paired_end.recalibrated.sorted.bintest.cns:md5,7a66b5f63acb05e6dfb0784c215851ec",
+                "test2.paired_end.recalibrated.sorted.call.cns:md5,f7caeca04aba28b125ce26b511f42afb",
+                "test2.paired_end.recalibrated.sorted.cnr:md5,d9bdb71ce807051369577ee7f807a40c",
+                "test2.paired_end.recalibrated.sorted.cns:md5,2b56aac606ba6183d018b30ca58afcec",
+                "test2.paired_end.recalibrated.sorted.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
+                "test2.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,e6d0190c1c37ce6e41f76ca5b24ccca3",
+                "test2.paired_end.recalibrated.sorted.tumor_only.call.cns:md5,70a308c6db7acf1a5fd623936cac6412",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f9e727147a7cbca46c2b9b2de3ecae91",
                 "reference.cnn:md5,e0aefc1c8a40e6919dc9cfb686e51f7c",
-                "sample3.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample3.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
-                "sample4.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample4.bintest.cns:md5,b5ccf48e99562082026e94f8d8aa1133",
-                "sample4.call.cns:md5,8202ec610e834c58bb7199761fccd9e5",
-                "sample4.cnr:md5,47d799d37af7ca8022fcac0ec705a3b8",
-                "sample4.cns:md5,50352818fd705424a7ab770d7707c312",
-                "sample4.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
-                "sample4.somatic.call.cns:md5,1cb4febd5c88d306277f3c4c3117d68c",
-                "sample4.targetcoverage.cnn:md5,62c2d1f8765618c454bf4455c2298344"
+                "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
+                "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test2.paired_end.recalibrated.sorted.bintest.cns:md5,b5ccf48e99562082026e94f8d8aa1133",
+                "test2.paired_end.recalibrated.sorted.call.cns:md5,8202ec610e834c58bb7199761fccd9e5",
+                "test2.paired_end.recalibrated.sorted.cnr:md5,47d799d37af7ca8022fcac0ec705a3b8",
+                "test2.paired_end.recalibrated.sorted.cns:md5,50352818fd705424a7ab770d7707c312",
+                "test2.paired_end.recalibrated.sorted.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
+                "test2.paired_end.recalibrated.sorted.somatic.call.cns:md5,1cb4febd5c88d306277f3c4c3117d68c",
+                "test2.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,62c2d1f8765618c454bf4455c2298344"
             ],
             "No BAM files",
             "No CRAM files",
@@ -458,9 +456,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:19:35.224650709"
+        "timestamp": "2026-07-20T10:18:52.335661"
     },
     "-profile test --tools cnvkit --input recalibrated_germline.csv": {
         "content": [
@@ -470,8 +468,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
@@ -620,9 +617,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:14:57.81598857"
+        "timestamp": "2026-07-20T10:05:28.442046"
     },
     "-profile test --tools cnvkit --input recalibrated_tumoronly.csv": {
         "content": [
@@ -734,17 +731,17 @@
                 "variant_calling/cnvkit/sample2",
                 "variant_calling/cnvkit/sample2/cnvkit.reference.antitarget-tmp.bed",
                 "variant_calling/cnvkit/sample2/cnvkit.reference.target-tmp.bed",
-                "variant_calling/cnvkit/sample2/sample2-diagram.pdf",
-                "variant_calling/cnvkit/sample2/sample2-scatter.png",
-                "variant_calling/cnvkit/sample2/sample2.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample2/sample2.bintest.cns",
-                "variant_calling/cnvkit/sample2/sample2.call.cns",
-                "variant_calling/cnvkit/sample2/sample2.cnr",
-                "variant_calling/cnvkit/sample2/sample2.cns",
                 "variant_calling/cnvkit/sample2/sample2.cnvcall.vcf",
-                "variant_calling/cnvkit/sample2/sample2.genemetrics.tsv",
-                "variant_calling/cnvkit/sample2/sample2.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample2/sample2.tumor_only.call.cns"
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted-diagram.pdf",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted-scatter.png",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.bintest.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.call.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.cnr",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.cns",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.genemetrics.tsv",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.targetcoverage.cnn",
+                "variant_calling/cnvkit/sample2/test2.paired_end.recalibrated.sorted.tumor_only.call.cns"
             ],
             [
                 "mosdepth-coverage-per-contig-single.txt:md5,ff38f1e35ce12244c751921db673b23c",
@@ -761,14 +758,14 @@
                 "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
                 "cnvkit.reference.antitarget-tmp.bed:md5,3d4d20f9f23b39970865d29ef239d20b",
                 "cnvkit.reference.target-tmp.bed:md5,657b25dbda8516624efa8cb2cf3716ca",
-                "sample2.antitargetcoverage.cnn:md5,067115082c4af4b64d58c0dc3a3642e4",
-                "sample2.bintest.cns:md5,7a66b5f63acb05e6dfb0784c215851ec",
-                "sample2.call.cns:md5,f7caeca04aba28b125ce26b511f42afb",
-                "sample2.cnr:md5,d9bdb71ce807051369577ee7f807a40c",
-                "sample2.cns:md5,2b56aac606ba6183d018b30ca58afcec",
-                "sample2.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
-                "sample2.targetcoverage.cnn:md5,e6d0190c1c37ce6e41f76ca5b24ccca3",
-                "sample2.tumor_only.call.cns:md5,70a308c6db7acf1a5fd623936cac6412"
+                "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,067115082c4af4b64d58c0dc3a3642e4",
+                "test2.paired_end.recalibrated.sorted.bintest.cns:md5,7a66b5f63acb05e6dfb0784c215851ec",
+                "test2.paired_end.recalibrated.sorted.call.cns:md5,f7caeca04aba28b125ce26b511f42afb",
+                "test2.paired_end.recalibrated.sorted.cnr:md5,d9bdb71ce807051369577ee7f807a40c",
+                "test2.paired_end.recalibrated.sorted.cns:md5,2b56aac606ba6183d018b30ca58afcec",
+                "test2.paired_end.recalibrated.sorted.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
+                "test2.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,e6d0190c1c37ce6e41f76ca5b24ccca3",
+                "test2.paired_end.recalibrated.sorted.tumor_only.call.cns:md5,70a308c6db7acf1a5fd623936cac6412"
             ],
             "No BAM files",
             "No CRAM files",
@@ -779,9 +776,9 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:17:53.424126221"
+        "timestamp": "2026-07-20T10:13:42.841328"
     },
     "-profile test --tools cnvkit --input recalibrated_tumoronly.csv --no_intervals": {
         "content": [
@@ -888,17 +885,16 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:21:53.321675509"
+        "timestamp": "2026-07-20T10:24:27.668069"
     },
     "-profile test --tools cnvkit --input recalibrated_somatic.csv --no_intervals": {
         "content": [
             16,
             {
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10",
-                    "samtools": 1.17
+                    "cnvkit": "0.9.10"
                 },
                 "CNVKIT_CALL": {
                     "cnvkit": "0.9.10"
@@ -1019,19 +1015,19 @@
                 "variant_calling/cnvkit/sample4_vs_sample3/genome.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/genome.target.bed",
                 "variant_calling/cnvkit/sample4_vs_sample3/reference.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample3.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-diagram.pdf",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4-scatter.png",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.antitargetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.bintest.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cnr",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.genemetrics.tsv",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.somatic.call.cns",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4.targetcoverage.cnn",
-                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf"
+                "variant_calling/cnvkit/sample4_vs_sample3/sample4_vs_sample3.cnvcall.vcf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test.paired_end.recalibrated.sorted.targetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-diagram.pdf",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted-scatter.png",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.bintest.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cnr",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.genemetrics.tsv",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.somatic.call.cns",
+                "variant_calling/cnvkit/sample4_vs_sample3/test2.paired_end.recalibrated.sorted.targetcoverage.cnn"
             ],
             [
                 "mosdepth-coverage-per-contig-single.txt:md5,336d786b273c4d4e714d51c44207ff56",
@@ -1067,16 +1063,16 @@
                 "genome.bed:md5,9c6cc178da8c2c27364be9f25c9df96d",
                 "genome.target.bed:md5,ee8081becc36524d35889e3b5f70961b",
                 "reference.cnn:md5,8c53491ff76a2a06b7a977714db862bb",
-                "sample3.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample3.targetcoverage.cnn:md5,0067cc3a0e479b23ab3bf056cead31b4",
-                "sample4.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
-                "sample4.bintest.cns:md5,b6701cceb525c3087f4dad432d20100c",
-                "sample4.call.cns:md5,2e0cc8813e274b25175b4346f4698fbb",
-                "sample4.cnr:md5,9dba016feb45f566a92e0aac184472bb",
-                "sample4.cns:md5,b3dfd6adf2ac97009dc460453d42659b",
-                "sample4.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
-                "sample4.somatic.call.cns:md5,5e4d472294661d2010e13deefe5ae4f5",
-                "sample4.targetcoverage.cnn:md5,1e1012812eb893afd931485cb760294e"
+                "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,0067cc3a0e479b23ab3bf056cead31b4",
+                "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
+                "test2.paired_end.recalibrated.sorted.bintest.cns:md5,b6701cceb525c3087f4dad432d20100c",
+                "test2.paired_end.recalibrated.sorted.call.cns:md5,2e0cc8813e274b25175b4346f4698fbb",
+                "test2.paired_end.recalibrated.sorted.cnr:md5,9dba016feb45f566a92e0aac184472bb",
+                "test2.paired_end.recalibrated.sorted.cns:md5,b3dfd6adf2ac97009dc460453d42659b",
+                "test2.paired_end.recalibrated.sorted.genemetrics.tsv:md5,5ec3555520f502f00f551ae7900a3824",
+                "test2.paired_end.recalibrated.sorted.somatic.call.cns:md5,5e4d472294661d2010e13deefe5ae4f5",
+                "test2.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,1e1012812eb893afd931485cb760294e"
             ],
             "No BAM files",
             "No CRAM files",
@@ -1088,8 +1084,8 @@
         ],
         "meta": {
             "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nextflow": "25.10.4"
         },
-        "timestamp": "2025-09-30T22:20:58.031095995"
+        "timestamp": "2026-07-20T10:22:29.609745"
     }
 }
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index 682969c7b4..978156f8df 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -349,7 +349,7 @@
             "No BAM files",
             "No CRAM files",
             [
-                "sample4_vs_sample3.MuSE.txt:md5,32961f7d718b4a1114f168253e50ef9e"
+                "sample4_vs_sample3.MuSE.txt:md5,015deb2b6fba5916733b16d51f5ee63b"
             ],
             [
                 "sample4_vs_sample3.muse.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e"
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 324c396371..8bb7129737 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -139,11 +139,11 @@ workflow SAREK {
     // PREPROCESSING
     if (step == 'mapping') {
         // Figure out if input is bam, fastq, or spring
-        input_sample_type = input_sample.branch {
-            bam: it[0].data_type == "bam"
-            fastq_gz: it[0].data_type == "fastq_gz"
-            one_fastq_gz_spring: it[0].data_type == "one_fastq_gz_spring"
-            two_fastq_gz_spring: it[0].data_type == "two_fastq_gz_spring"
+        input_sample_type = input_sample.branch { sample ->
+            bam: sample[0].data_type == "bam"
+            fastq_gz: sample[0].data_type == "fastq_gz"
+            one_fastq_gz_spring: sample[0].data_type == "one_fastq_gz_spring"
+            two_fastq_gz_spring: sample[0].data_type == "two_fastq_gz_spring"
         }
 
         // Two fastq.gz-files
@@ -289,7 +289,7 @@ workflow SAREK {
         if (tools.split(',').contains('cnvkit') || tools.split(',').contains('msisensor2') || tools.split(',').contains('muse')) {
 
             // Differentiate between bam and cram files
-            cram_variant_calling_status_tmp = cram_variant_calling.branch { meta, file, index ->
+            cram_variant_calling_status_tmp = cram_variant_calling.branch { _meta, file, _index ->
                 bam: file.toString().endsWith('.bam')
                 cram: file.toString().endsWith('.cram')
             }
@@ -309,13 +309,13 @@ workflow SAREK {
         }
 
         // Logic to separate germline samples, tumor samples with no matched normal, and combine tumor-normal pairs
-        cram_variant_calling_status = cram_variant_calling.branch { meta, file, index ->
+        cram_variant_calling_status = cram_variant_calling.branch { meta, _file, _index ->
             normal: meta.status == 0
             tumor: meta.status == 1
         }
 
         // Follow the same logic with bam as we have with cram
-        bam_variant_calling_status = bam_variant_calling.branch { meta, file, index ->
+        bam_variant_calling_status = bam_variant_calling.branch { meta, _file, _index ->
             normal: meta.status == 0
             tumor: meta.status == 1
         }
@@ -340,13 +340,13 @@ workflow SAREK {
         bam_variant_calling_tumor_joined = bam_variant_calling_tumor_grouped.join(bam_variant_calling_normal_to_cross, failOnDuplicate: true, remainder: true)
 
         // 3. Filter out entries with last entry null
-        cram_variant_calling_tumor_filtered = cram_variant_calling_tumor_joined.filter { it -> !(it.last()) }
-        bam_variant_calling_tumor_filtered = bam_variant_calling_tumor_joined.filter { it -> !(it.last()) }
+        cram_variant_calling_tumor_filtered = cram_variant_calling_tumor_joined.filter { joined -> !(joined.last()) }
+        bam_variant_calling_tumor_filtered = bam_variant_calling_tumor_joined.filter { joined -> !(joined.last()) }
 
         // 4. Transpose [ patient1, [ meta1, meta2 ], [ cram1, crai1, cram2, crai2 ] ] back to [ patient1, meta1, [ cram1, crai1 ], null ] [ patient1, meta2, [ cram2, crai2 ], null ]
         // and remove patient ID field & null value for further processing [ meta1, [ cram1, crai1 ] ] [ meta2, [ cram2, crai2 ] ]
-        cram_variant_calling_tumor_only = cram_variant_calling_tumor_filtered.transpose().map { it -> [it[1], it[2], it[3]] }
-        bam_variant_calling_tumor_only = bam_variant_calling_tumor_filtered.transpose().map { it -> [it[1], it[2], it[3]] }
+        cram_variant_calling_tumor_only = cram_variant_calling_tumor_filtered.transpose().map { record -> [record[1], record[2], record[3]] }
+        bam_variant_calling_tumor_only = bam_variant_calling_tumor_filtered.transpose().map { record -> [record[1], record[2], record[3]] }
 
         if (params.only_paired_variant_calling) {
             // Normal only samples
@@ -356,12 +356,12 @@ workflow SAREK {
             bam_variant_calling_normal_joined = bam_variant_calling_normal_to_cross.join(bam_variant_calling_tumor_grouped, failOnDuplicate: true, remainder: true)
 
             // 2. Filter out entries with last entry null
-            cram_variant_calling_normal_filtered = cram_variant_calling_normal_joined.filter { it -> !(it.last()) }
-            bam_variant_calling_normal_filtered = bam_variant_calling_normal_joined.filter { it -> !(it.last()) }
+            cram_variant_calling_normal_filtered = cram_variant_calling_normal_joined.filter { joined -> !(joined.last()) }
+            bam_variant_calling_normal_filtered = bam_variant_calling_normal_joined.filter { joined -> !(joined.last()) }
 
             // 3. Remove patient ID field & null value for further processing [ meta1, [ cram1, crai1 ] ] [ meta2, [ cram2, crai2 ] ] (no transposing needed since only one normal per patient ID)
-            cram_variant_calling_status_normal = cram_variant_calling_normal_filtered.map { it -> [it[1], it[2], it[3]] }
-            bam_variant_calling_status_normal = bam_variant_calling_normal_filtered.map { it -> [it[1], it[2], it[3]] }
+            cram_variant_calling_status_normal = cram_variant_calling_normal_filtered.map { record -> [record[1], record[2], record[3]] }
+            bam_variant_calling_status_normal = bam_variant_calling_normal_filtered.map { record -> [record[1], record[2], record[3]] }
         }
         else {
             cram_variant_calling_status_normal = cram_variant_calling_status.normal
@@ -458,7 +458,6 @@ workflow SAREK {
             germline_resource,
             germline_resource_tbi,
             intervals_and_num_intervals,
-            intervals_bed_gz_tbi_and_num_intervals,
             intervals_bed_combined,
             intervals_bed_gz_tbi_combined,
             mappability,
@@ -708,8 +707,8 @@ def flowcellLaneFromFastq(path) {
 def readFirstLineOfFastq(path) {
     def line = null
     try {
-        path.withInputStream {
-            def InputStream gzipStream = new java.util.zip.GZIPInputStream(it)
+        path.withInputStream { stream ->
+            def InputStream gzipStream = new java.util.zip.GZIPInputStream(stream)
             def Reader decoder = new InputStreamReader(gzipStream, 'ASCII')
             def BufferedReader buffered = new BufferedReader(decoder)
             line = buffered.readLine()

From 7b2af90d102b1fe613ff72b146a52653badfedd0 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Famke=20B=C3=A4uerle?=
 <45968370+famosab@users.noreply.github.com>
Date: Tue, 21 Jul 2026 17:35:06 +0200
Subject: [PATCH 13/27] Update muse/sump to check if the index is older before
 touching it (#2237)

<!--
# nf-core/sarek pull request

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Please fill in the appropriate checklist below (delete whatever is not
relevant).
These are the most common things requested on pull requests (PRs).

Remember that PRs should be made against the dev branch, unless you're
preparing a pipeline release.

Learn more about contributing:
[CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
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## PR checklist

Closes #2236

- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [ ] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [ ] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).
---
 CHANGELOG.md                      | 1 +
 modules.json                      | 4 ++--
 modules/nf-core/muse/call/main.nf | 2 +-
 modules/nf-core/muse/sump/main.nf | 7 ++++---
 4 files changed, 8 insertions(+), 6 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 122f2710a1..019892bb2c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -67,6 +67,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2229](https://github.com/nf-core/sarek/pull/2229) - Update nft-utils to 1.0.0, migrate `getAllFilesFromDir` to `getAllFilesFromPath` in test utilities
 - [#2233](https://github.com/nf-core/sarek/pull/2233) - Replace the last remaining deprecated `channel.from` with `channel.of` in `prepare_genome`
 - [#2234](https://github.com/nf-core/sarek/pull/2234) - Replace deprecated `.set {}` channel terminator with plain assignment in local subworkflows for Nextflow strict-syntax / 26.x readiness
+- [#2237](https://github.com/nf-core/sarek/pull/2237) - Update muse/sump module to check if the index is older before touching it
 
 #### Fixed
 
diff --git a/modules.json b/modules.json
index bc9f66c29c..62fcdbdc14 100644
--- a/modules.json
+++ b/modules.json
@@ -393,12 +393,12 @@
                     },
                     "muse/call": {
                         "branch": "master",
-                        "git_sha": "2ae2df92d6f5ac2d54058d0cece928e13d241912",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "muse/sump": {
                         "branch": "master",
-                        "git_sha": "2ae2df92d6f5ac2d54058d0cece928e13d241912",
+                        "git_sha": "e8c96d814d65d575a840063c90905e44ac5ecc12",
                         "installed_by": ["modules"]
                     },
                     "ngscheckmate/ncm": {
diff --git a/modules/nf-core/muse/call/main.nf b/modules/nf-core/muse/call/main.nf
index 0f8ceb3c9f..cb7025f3ec 100644
--- a/modules/nf-core/muse/call/main.nf
+++ b/modules/nf-core/muse/call/main.nf
@@ -3,7 +3,7 @@ process MUSE_CALL {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9f/9f0ebb574ef5eed2a6e034f1b2feea6c252d1ab0c8bc5135a669059aa1f4d2ca/data'
         : 'community.wave.seqera.io/library/muse:6637291dcbb0bdb8'}"
 
diff --git a/modules/nf-core/muse/sump/main.nf b/modules/nf-core/muse/sump/main.nf
index 3758ad38a7..cc9adbbd8d 100644
--- a/modules/nf-core/muse/sump/main.nf
+++ b/modules/nf-core/muse/sump/main.nf
@@ -3,7 +3,7 @@ process MUSE_SUMP {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/83/83d1d3caa1b6ce54ce999e0061d7fe8acbe6788d5c7970574eff330ea819fb85/data'
         : 'community.wave.seqera.io/library/htslib_muse:9a4b9cb78c211f1e'}"
 
@@ -25,9 +25,10 @@ process MUSE_SUMP {
     // args for bgzip
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    // MuSE complains if the timestamp of the dbsnp VCF index is older than the timestamp of the VCF itself, so we need to touch it here
+    // MuSE complains if the timestamp of the dbsnp VCF index is older than the timestamp of the VCF itself
+    // we check whether that is the case and if its not then we run touch
     """
-    touch ${ref_vcf_tbi}
+    [ "${ref_vcf_tbi}" -ot "${ref_vcf}" ] && touch "${ref_vcf_tbi}"
 
     MuSE \\
         sump \\

From a7377e65fc75b80057769bc22bc3a763527e945b Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Wed, 22 Jul 2026 15:14:04 +0200
Subject: [PATCH 14/27] chore(modules): migrate gatk4/gatk4spark to versions
 topic channel (#2238)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates all `gatk4` and `gatk4spark` modules to the `versions` topic
channel (updated to nf-core/modules latest via nf-core/tools 4.0.2).

### Changes
- Updates 24 modules (21 `gatk4` + 3 `gatk4spark`) to their
topic-channel versions.
- Removes the corresponding `.mix(<module>.out.versions)` wiring from
subworkflows — versions now flow through `channel.topic("versions")`
(already consumed in `workflows/sarek.nf`).
- Fixes the `MUTECT2` / `MUTECT2_PAIRED` call sites for the new input
signature: `gzi` added to the fai tuple, new `alleles` / `alleles_tbi`
inputs passed as `[]` (unused in sarek).
- `gatk4spark` tool bump 4.6.1.0 → 4.6.2.0 (rides along with the module
update).

### Notes
- Test snapshots still need regenerating in CI.
- First of a stacked series migrating the remaining modules to the
versions topic channel; subsequent PRs are based on this branch.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   3 +
 modules.json                                  |  48 +++---
 .../nf-core/gatk4/applybqsr/environment.yml   |   3 +-
 modules/nf-core/gatk4/applybqsr/main.nf       |  22 +--
 modules/nf-core/gatk4/applybqsr/meta.yml      |  26 +++-
 .../nf-core/gatk4/applyvqsr/environment.yml   |   3 +-
 modules/nf-core/gatk4/applyvqsr/main.nf       |  47 +++---
 modules/nf-core/gatk4/applyvqsr/meta.yml      |  77 ++++++---
 .../gatk4/baserecalibrator/environment.yml    |   3 +-
 .../nf-core/gatk4/baserecalibrator/main.nf    |  40 ++---
 .../nf-core/gatk4/baserecalibrator/meta.yml   |  44 ++++--
 .../calculatecontamination/environment.yml    |   3 +-
 .../gatk4/calculatecontamination/main.nf      |  39 ++---
 .../gatk4/calculatecontamination/meta.yml     |  45 ++++--
 .../nf-core/gatk4/cnnscorevariants/main.nf    |  50 +++---
 .../nf-core/gatk4/cnnscorevariants/meta.yml   |  87 +++++++----
 .../createsequencedictionary/environment.yml  |   3 +-
 .../gatk4/createsequencedictionary/main.nf    |  18 +--
 .../gatk4/createsequencedictionary/meta.yml   |  26 +++-
 .../estimatelibrarycomplexity/environment.yml |   3 +-
 .../gatk4/estimatelibrarycomplexity/main.nf   |  41 ++---
 .../gatk4/estimatelibrarycomplexity/meta.yml  |  59 ++++---
 .../gatk4/filtermutectcalls/environment.yml   |   3 +-
 .../nf-core/gatk4/filtermutectcalls/main.nf   |  53 +++----
 .../nf-core/gatk4/filtermutectcalls/meta.yml  |  68 ++++++--
 .../filtervarianttranches/environment.yml     |   3 +-
 .../gatk4/filtervarianttranches/main.nf       |  38 ++---
 .../gatk4/filtervarianttranches/meta.yml      |  88 +++++++----
 .../gatk4/gatherbqsrreports/environment.yml   |   3 +-
 .../nf-core/gatk4/gatherbqsrreports/main.nf   |  32 ++--
 .../nf-core/gatk4/gatherbqsrreports/meta.yml  |  32 +++-
 .../gatherpileupsummaries/environment.yml     |   3 +-
 .../gatk4/gatherpileupsummaries/main.nf       |  38 ++---
 .../gatk4/gatherpileupsummaries/meta.yml      |  39 +++--
 .../gatk4/genomicsdbimport/environment.yml    |   3 +-
 .../nf-core/gatk4/genomicsdbimport/main.nf    |  59 +++----
 .../nf-core/gatk4/genomicsdbimport/meta.yml   | 114 +++++++-------
 .../gatk4/genotypegvcfs/environment.yml       |   3 +-
 modules/nf-core/gatk4/genotypegvcfs/main.nf   |  45 +++---
 modules/nf-core/gatk4/genotypegvcfs/meta.yml  |  48 ++++--
 .../gatk4/getpileupsummaries/environment.yml  |   3 +-
 .../nf-core/gatk4/getpileupsummaries/main.nf  |  45 +++---
 .../nf-core/gatk4/getpileupsummaries/meta.yml |  58 +++++--
 .../gatk4/haplotypecaller/environment.yml     |   3 +-
 modules/nf-core/gatk4/haplotypecaller/main.nf |  57 +++----
 .../nf-core/gatk4/haplotypecaller/meta.yml    |  51 ++++--
 .../gatk4/intervallisttobed/environment.yml   |   3 +-
 .../nf-core/gatk4/intervallisttobed/main.nf   |  18 +--
 .../nf-core/gatk4/intervallisttobed/meta.yml  |  31 +++-
 .../learnreadorientationmodel/environment.yml |   3 +-
 .../gatk4/learnreadorientationmodel/main.nf   |  32 ++--
 .../gatk4/learnreadorientationmodel/meta.yml  |  34 +++-
 .../gatk4/markduplicates/environment.yml      |   5 +-
 modules/nf-core/gatk4/markduplicates/main.nf  |  23 +--
 modules/nf-core/gatk4/markduplicates/meta.yml | 105 +++++++++----
 .../gatk4/mergemutectstats/environment.yml    |   3 +-
 .../nf-core/gatk4/mergemutectstats/main.nf    |  33 ++--
 .../nf-core/gatk4/mergemutectstats/meta.yml   |  32 +++-
 modules/nf-core/gatk4/mutect2/environment.yml |   3 +-
 modules/nf-core/gatk4/mutect2/main.nf         |  73 ++++-----
 modules/nf-core/gatk4/mutect2/meta.yml        | 147 +++++++++++-------
 .../gatk4/variantrecalibrator/environment.yml |   3 +-
 .../nf-core/gatk4/variantrecalibrator/main.nf |  59 +++----
 .../gatk4/variantrecalibrator/meta.yml        | 127 +++++++++------
 .../gatk4spark/applybqsr/environment.yml      |   3 +-
 modules/nf-core/gatk4spark/applybqsr/main.nf  |  22 +--
 modules/nf-core/gatk4spark/applybqsr/meta.yml |  31 +++-
 .../baserecalibrator/environment.yml          |   3 +-
 .../gatk4spark/baserecalibrator/main.nf       |  20 +--
 .../gatk4spark/baserecalibrator/meta.yml      |  80 ++++++----
 .../gatk4spark/markduplicates/environment.yml |   3 +-
 .../nf-core/gatk4spark/markduplicates/main.nf |  24 +--
 .../gatk4spark/markduplicates/meta.yml        |  69 +++++---
 subworkflows/local/bam_applybqsr/main.nf      |   1 -
 .../local/bam_applybqsr_spark/main.nf         |   1 -
 .../local/bam_baserecalibrator/main.nf        |   5 -
 .../local/bam_baserecalibrator_spark/main.nf  |   5 -
 .../bam_joint_calling_germline_gatk/main.nf   |   6 -
 subworkflows/local/bam_markduplicates/main.nf |   1 -
 .../local/bam_markduplicates_spark/main.nf    |   2 -
 .../bam_variant_calling_germline_all/main.nf  |   5 -
 .../main.nf                                   |   3 -
 .../bam_variant_calling_somatic_all/main.nf   |   1 -
 .../main.nf                                   |  15 +-
 .../main.nf                                   |   1 -
 .../main.nf                                   |  13 +-
 .../local/fastq_preprocess_gatk/main.nf       |   2 -
 subworkflows/local/prepare_genome/main.nf     |   1 -
 subworkflows/local/prepare_intervals/main.nf  |   1 -
 .../local/vcf_variant_filtering_gatk/main.nf  |   4 -
 tests/aligner-bwa-mem.nf.test.snap            |   8 +-
 tests/aligner-bwa-mem2.nf.test.snap           |   8 +-
 tests/aligner-dragmap.nf.test.snap            |   8 +-
 tests/aligner-parabricks.nf.test.snap         |  18 +--
 tests/alignment_from_everything.nf.test.snap  |  10 +-
 tests/alignment_to_fastq.nf.test.snap         |  10 +-
 tests/bbsplit.nf.test.snap                    |  26 ++--
 tests/default.nf.test.snap                    |  20 +--
 tests/fastp.nf.test.snap                      |  30 ++--
 ...joint_calling_haplotypecaller.nf.test.snap |  12 +-
 tests/joint_calling_mutect2.nf.test.snap      |  12 +-
 tests/lane_integer.nf.test.snap               |   2 +-
 tests/multi_lane.nf.test.snap                 |  12 +-
 tests/postprocess_concatenation.nf.test.snap  |   2 +-
 ...s_concatenation_normalization.nf.test.snap |   4 +-
 tests/postprocess_consensus.nf.test.snap      |  26 ++--
 tests/postprocess_filtering.nf.test.snap      |   2 +-
 tests/postprocess_normalization.nf.test.snap  |   2 +-
 tests/postprocess_varlociraptor.nf.test.snap  |  20 +--
 tests/save_mapped.nf.test.snap                |   2 +-
 tests/save_output_as_bam.nf.test.snap         |  14 +-
 tests/sentieon.nf.test.snap                   |  10 +-
 tests/sentieon_aligner_bwamem.nf.test.snap    |   6 +-
 tests/sentieon_dedup.nf.test.snap             |  30 ++--
 tests/spark.nf.test.snap                      |  18 +--
 tests/start_from_markduplicates.nf.test.snap  |  32 ++--
 ...art_from_preparerecalibration.nf.test.snap |  16 +-
 tests/start_from_recalibration.nf.test.snap   |  12 +-
 tests/tumor-normal-pair.nf.test.snap          |  10 +-
 tests/umi_fastp.nf.test.snap                  |  10 +-
 tests/umi_fgbio.nf.test.snap                  |  10 +-
 tests/umi_in_read_names.nf.test.snap          |  20 +--
 tests/variant_calling_all.nf.test.snap        |  40 ++---
 .../variant_calling_deepvariant.nf.test.snap  |   4 +-
 tests/variant_calling_freebayes.nf.test.snap  |  40 ++---
 ...riant_calling_haplotypecaller.nf.test.snap |  12 +-
 tests/variant_calling_lofreq.nf.test.snap     |   4 +-
 tests/variant_calling_msisensor2.nf.test.snap |   6 +-
 .../variant_calling_msisensorpro.nf.test.snap |   6 +-
 tests/variant_calling_muse.nf.test.snap       |   4 +-
 tests/variant_calling_mutect2.nf.test.snap    |  24 +--
 ...ant_calling_sentieon_dnascope.nf.test.snap |   6 +-
 ...ling_sentieon_haplotypecaller.nf.test.snap |  10 +-
 ...iant_calling_sentieon_tnscope.nf.test.snap |   6 +-
 134 files changed, 1789 insertions(+), 1430 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 019892bb2c..f6ccbf673a 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -18,6 +18,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2232](https://github.com/nf-core/sarek/pull/2232) - Migrate local code to the lowercase `channel` factory for Nextflow strict-syntax / 26.x readiness
 - [#2235](https://github.com/nf-core/sarek/pull/2235) - Nextflow strict-syntax / 26.x readiness for local code: explicit, named closure parameters (replacing implicit/generic `it`), `_`-prefixed unused parameters, and removal of unused `take:` inputs. Previously-dropped Manta candidate VCFs and Sentieon gVCF indices are now emitted.
 - [#2235](https://github.com/nf-core/sarek/pull/2235) - germline CNVKIT reuses the shared `CRAM_TO_BAM` conversion instead of re-converting CRAM internally, avoiding a duplicate conversion. Side effect: with `--step variant_calling` (user-supplied CRAM/BAM), CNVKit output files are named after the input file rather than the sample; runs from FASTQ are unaffected.
+- [#2238](https://github.com/nf-core/sarek/pull/2238) - Migrate `gatk4`/`gatk4spark` modules to the versions topic channel (bumps gatk4spark 4.6.1.0 → 4.6.2.0)
 
 ### Fixed
 
@@ -34,6 +35,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | htslib        | 1.21        | 1.23.1      |
 | varlociraptor | 8.9.3       | 8.9.5       |
 | ensembl-vep   | 115.2       | 116.0       |
+| gatk4         | 4.6.1.0     | 4.6.2.0     |
+| gatk4-spark   | 4.6.1.0     | 4.6.2.0     |
 
 ### Dependencies - plugins
 
diff --git a/modules.json b/modules.json
index 62fcdbdc14..6ffabb8f5a 100644
--- a/modules.json
+++ b/modules.json
@@ -208,97 +208,97 @@
                     },
                     "gatk4/applybqsr": {
                         "branch": "master",
-                        "git_sha": "620300d96c4b9051d533a5ea5f9d3b2c64b781ce",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/applyvqsr": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/baserecalibrator": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/calculatecontamination": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/cnnscorevariants": {
                         "branch": "master",
-                        "git_sha": "1999eff2c530b2b185a25cc42117a1686f09b685",
+                        "git_sha": "21e4662ae927fdbc5fc04eec9bde38b0689d4954",
                         "installed_by": ["modules"]
                     },
                     "gatk4/createsequencedictionary": {
                         "branch": "master",
-                        "git_sha": "a57253204b8f4022edfeaa3ae2f5e2abecd8858b",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/estimatelibrarycomplexity": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/filtermutectcalls": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/filtervarianttranches": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/gatherbqsrreports": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/gatherpileupsummaries": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/genomicsdbimport": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/genotypegvcfs": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/getpileupsummaries": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/haplotypecaller": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "7abcc95130dd0388f696002a99adeb59eca54471",
                         "installed_by": ["modules"]
                     },
                     "gatk4/intervallisttobed": {
                         "branch": "master",
-                        "git_sha": "20fe8646005253d57a7a8db42abf69ea0966dc75",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/learnreadorientationmodel": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/markduplicates": {
                         "branch": "master",
-                        "git_sha": "1ec937ab3edc307bc0d79a2200d784e9f0868359",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/mergemutectstats": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4/mergevcfs": {
@@ -308,27 +308,27 @@
                     },
                     "gatk4/mutect2": {
                         "branch": "master",
-                        "git_sha": "a97cba262e9367734e435dc07d2e3b7d6121ef3e",
+                        "git_sha": "f8001d415ee08634fee5019bd15c7fdaa2a1915d",
                         "installed_by": ["modules"]
                     },
                     "gatk4/variantrecalibrator": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4spark/applybqsr": {
                         "branch": "master",
-                        "git_sha": "620300d96c4b9051d533a5ea5f9d3b2c64b781ce",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4spark/baserecalibrator": {
                         "branch": "master",
-                        "git_sha": "fa55ebb81654fe1736975fa28d1af5a079bf6a08",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gatk4spark/markduplicates": {
                         "branch": "master",
-                        "git_sha": "fa55ebb81654fe1736975fa28d1af5a079bf6a08",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "gawk": {
diff --git a/modules/nf-core/gatk4/applybqsr/environment.yml b/modules/nf-core/gatk4/applybqsr/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/applybqsr/environment.yml
+++ b/modules/nf-core/gatk4/applybqsr/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/applybqsr/main.nf b/modules/nf-core/gatk4/applybqsr/main.nf
index 75b7be0594..4829d563cb 100644
--- a/modules/nf-core/gatk4/applybqsr/main.nf
+++ b/modules/nf-core/gatk4/applybqsr/main.nf
@@ -3,9 +3,9 @@ process GATK4_APPLYBQSR {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data'
-        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals)
@@ -14,10 +14,10 @@ process GATK4_APPLYBQSR {
     path dict
 
     output:
-    tuple val(meta), path("${prefix}.bam"),  emit: bam,  optional: true
-    tuple val(meta), path("${prefix}*bai"),  emit: bai,  optional: true
+    tuple val(meta), path("${prefix}.bam"), emit: bam, optional: true
+    tuple val(meta), path("${prefix}*bai"), emit: bai, optional: true
     tuple val(meta), path("${prefix}.cram"), emit: cram, optional: true
-    path "versions.yml",                     emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -46,11 +46,6 @@ process GATK4_APPLYBQSR {
         ${interval_command} \\
         --tmp-dir . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -63,10 +58,5 @@ process GATK4_APPLYBQSR {
     else
         touch ${prefix}.bai
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/applybqsr/meta.yml b/modules/nf-core/gatk4/applybqsr/meta.yml
index 6e7bedad71..3f926ce1c6 100644
--- a/modules/nf-core/gatk4/applybqsr/meta.yml
+++ b/modules/nf-core/gatk4/applybqsr/meta.yml
@@ -90,13 +90,27 @@ output:
           description: Recalibrated CRAM file
           pattern: "${prefix}.cram"
           ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@yocra3"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4/applyvqsr/environment.yml b/modules/nf-core/gatk4/applyvqsr/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/applyvqsr/environment.yml
+++ b/modules/nf-core/gatk4/applyvqsr/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/applyvqsr/main.nf b/modules/nf-core/gatk4/applyvqsr/main.nf
index c8ea3da5bf..9e42f44c87 100644
--- a/modules/nf-core/gatk4/applyvqsr/main.nf
+++ b/modules/nf-core/gatk4/applyvqsr/main.nf
@@ -1,22 +1,22 @@
 process GATK4_APPLYVQSR {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(vcf), path(vcf_tbi), path(recal), path(recal_index), path(tranches)
-    path  fasta
-    path  fai
-    path  dict
+    path fasta
+    path fai
+    path dict
 
     output:
     tuple val(meta), path("*.vcf.gz"), emit: vcf
-    tuple val(meta), path("*.tbi")   , emit: tbi
-    path "versions.yml"              , emit: versions
+    tuple val(meta), path("*.tbi"), emit: tbi
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,40 +24,31 @@ process GATK4_APPLYVQSR {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def reference_command = fasta ? "--reference $fasta" : ''
+    def reference_command = fasta ? "--reference ${fasta}" : ''
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK ApplyVQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK ApplyVQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         ApplyVQSR \\
         --variant ${vcf} \\
         --output ${prefix}.vcf.gz \\
-        $reference_command \\
-        --tranches-file $tranches \\
-        --recal-file $recal \\
+        ${reference_command} \\
+        --tranches-file ${tranches} \\
+        --recal-file ${recal} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     echo "" | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/applyvqsr/meta.yml b/modules/nf-core/gatk4/applyvqsr/meta.yml
index ceedff621e..a31969c7d6 100644
--- a/modules/nf-core/gatk4/applyvqsr/meta.yml
+++ b/modules/nf-core/gatk4/applyvqsr/meta.yml
@@ -31,60 +31,91 @@ input:
         description: VCF file to be recalibrated, this should be the same file as used
           for the first stage VariantRecalibrator.
         pattern: "*.vcf"
+        ontologies: []
     - vcf_tbi:
         type: file
         description: tabix index for the input vcf file.
         pattern: "*.vcf.tbi"
+        ontologies: []
     - recal:
         type: file
         description: Recalibration file produced when the input vcf was run through
           VariantRecalibrator in stage 1.
         pattern: "*.recal"
+        ontologies: []
     - recal_index:
         type: file
         description: Index file for the recalibration file.
         pattern: ".recal.idx"
+        ontologies: []
     - tranches:
         type: file
         description: Tranches file produced when the input vcf was run through VariantRecalibrator
           in stage 1.
         pattern: ".tranches"
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
+        ontologies: []
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: file
           description: compressed vcf file containing the recalibrated variants.
           pattern: "*.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.vcf.gz":
           type: file
           description: compressed vcf file containing the recalibrated variants.
           pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
           type: file
-          description: Index of recalibrated vcf file.
-          pattern: "*vcf.gz.tbi"
+          description: compressed vcf file containing the recalibrated variants.
+          pattern: "*.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.tbi":
           type: file
           description: Index of recalibrated vcf file.
           pattern: "*vcf.gz.tbi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions.
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
 maintainers:
diff --git a/modules/nf-core/gatk4/baserecalibrator/environment.yml b/modules/nf-core/gatk4/baserecalibrator/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/baserecalibrator/environment.yml
+++ b/modules/nf-core/gatk4/baserecalibrator/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/baserecalibrator/main.nf b/modules/nf-core/gatk4/baserecalibrator/main.nf
index 493533c726..d7c5c65dd8 100644
--- a/modules/nf-core/gatk4/baserecalibrator/main.nf
+++ b/modules/nf-core/gatk4/baserecalibrator/main.nf
@@ -1,11 +1,11 @@
 process GATK4_BASERECALIBRATOR {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(intervals)
@@ -17,7 +17,7 @@ process GATK4_BASERECALIBRATOR {
 
     output:
     tuple val(meta), path("*.table"), emit: table
-    path "versions.yml"             , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,39 +25,31 @@ process GATK4_BASERECALIBRATOR {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def interval_command = intervals ? "--intervals $intervals" : ""
-    def sites_command = known_sites.collect{"--known-sites $it"}.join(' ')
+    def interval_command = intervals ? "--intervals ${intervals}" : ""
+    def sites_command = known_sites.collect { vcf -> "--known-sites ${vcf}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK BaseRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK BaseRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         BaseRecalibrator  \\
-        --input $input \\
+        --input ${input} \\
         --output ${prefix}.table \\
-        --reference $fasta \\
-        $interval_command \\
-        $sites_command \\
+        --reference ${fasta} \\
+        ${interval_command} \\
+        ${sites_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.table
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/baserecalibrator/meta.yml b/modules/nf-core/gatk4/baserecalibrator/meta.yml
index c3caeb8084..af53133648 100644
--- a/modules/nf-core/gatk4/baserecalibrator/meta.yml
+++ b/modules/nf-core/gatk4/baserecalibrator/meta.yml
@@ -27,13 +27,16 @@ input:
         type: file
         description: BAM/CRAM file from alignment
         pattern: "*.{bam,cram}"
+        ontologies: []
     - input_index:
         type: file
         description: BAI/CRAI file from alignment
         pattern: "*.{bai,crai}"
+        ontologies: []
     - intervals:
         type: file
         description: Bed file with the genomic regions included in the library (optional)
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -43,6 +46,7 @@ input:
         type: file
         description: The reference fasta file
         pattern: "*.fasta"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -52,6 +56,7 @@ input:
         type: file
         description: Index of reference fasta file
         pattern: "*.fasta.fai"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -61,6 +66,7 @@ input:
         type: file
         description: GATK sequence dictionary
         pattern: "*.dict"
+        ontologies: []
   - - meta5:
         type: map
         description: |
@@ -68,8 +74,10 @@ input:
           e.g. [ id:'genome']
     - known_sites:
         type: file
-        description: VCF files with known sites for indels / snps (optional)
+        description: VCF files with known sites for indels / snps
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
   - - meta6:
         type: map
         description: |
@@ -77,11 +85,12 @@ input:
           e.g. [ id:'genome']
     - known_sites_tbi:
         type: file
-        description: Tabix index of the known_sites (optional)
+        description: Tabix index of the known_sites
         pattern: "*.vcf.gz.tbi"
+        ontologies: []
 output:
-  - table:
-      - meta:
+  table:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -90,11 +99,28 @@ output:
           type: file
           description: Recalibration table from BaseRecalibrator
           pattern: "*.{table}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@yocra3"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4/calculatecontamination/environment.yml b/modules/nf-core/gatk4/calculatecontamination/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/calculatecontamination/environment.yml
+++ b/modules/nf-core/gatk4/calculatecontamination/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/calculatecontamination/main.nf b/modules/nf-core/gatk4/calculatecontamination/main.nf
index 20fe3c5e13..2601a69d87 100644
--- a/modules/nf-core/gatk4/calculatecontamination/main.nf
+++ b/modules/nf-core/gatk4/calculatecontamination/main.nf
@@ -1,19 +1,19 @@
 process GATK4_CALCULATECONTAMINATION {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(pileup), path(matched)
 
     output:
     tuple val(meta), path('*.contamination.table'), emit: contamination
-    tuple val(meta), path('*.segmentation.table') , emit: segmentation, optional:true
-    path "versions.yml"                           , emit: versions
+    tuple val(meta), path('*.segmentation.table'), emit: segmentation, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -21,38 +21,29 @@ process GATK4_CALCULATECONTAMINATION {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def matched_command = matched ? "--matched-normal $matched" : ''
+    def matched_command = matched ? "--matched-normal ${matched}" : ''
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK CalculateContamination] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK CalculateContamination] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         CalculateContamination \\
-        --input $pileup \\
+        --input ${pileup} \\
         --output ${prefix}.contamination.table \\
-        $matched_command \\
+        ${matched_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.contamination.table
     touch ${prefix}.segmentation.table
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/calculatecontamination/meta.yml b/modules/nf-core/gatk4/calculatecontamination/meta.yml
index ee90a48252..f07240b569 100644
--- a/modules/nf-core/gatk4/calculatecontamination/meta.yml
+++ b/modules/nf-core/gatk4/calculatecontamination/meta.yml
@@ -29,38 +29,59 @@ input:
         description: File containing the pileups summary table of a tumor sample to
           be used to calculate contamination.
         pattern: "*.pileups.table"
+        ontologies: []
     - matched:
         type: file
         description: File containing the pileups summary table of a normal sample that
           matches with the tumor sample specified in pileup argument. This is an optional
           input.
         pattern: "*.pileups.table"
+        ontologies: []
 output:
-  - contamination:
-      - meta:
+  contamination:
+    - - meta:
           type: file
           description: File containing the contamination table.
           pattern: "*.contamination.table"
+          ontologies: []
       - "*.contamination.table":
           type: file
           description: File containing the contamination table.
           pattern: "*.contamination.table"
-  - segmentation:
-      - meta:
+          ontologies: []
+  segmentation:
+    - - meta:
           type: file
-          description: output table containing segmentation of tumor minor allele fractions
-            (optional)
-          pattern: "*.segmentation.table"
+          description: File containing the contamination table.
+          pattern: "*.contamination.table"
+          ontologies: []
       - "*.segmentation.table":
           type: file
           description: output table containing segmentation of tumor minor allele fractions
             (optional)
           pattern: "*.segmentation.table"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
   - "@maxulysse"
diff --git a/modules/nf-core/gatk4/cnnscorevariants/main.nf b/modules/nf-core/gatk4/cnnscorevariants/main.nf
index 5ff13b41d1..6622a6f405 100644
--- a/modules/nf-core/gatk4/cnnscorevariants/main.nf
+++ b/modules/nf-core/gatk4/cnnscorevariants/main.nf
@@ -1,9 +1,9 @@
 process GATK4_CNNSCOREVARIANTS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     //Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811
-    container "nf-core/gatk:4.5.0.0" //Biocontainers is missing a package
+    container "quay.io/nf-core/gatk:4.5.0.0"
 
     input:
     tuple val(meta), path(vcf), path(tbi), path(aligned_input), path(intervals)
@@ -14,9 +14,9 @@ process GATK4_CNNSCOREVARIANTS {
     path weights
 
     output:
-    tuple val(meta), path("*cnn.vcf.gz")    , emit: vcf
+    tuple val(meta), path("*cnn.vcf.gz"), emit: vcf
     tuple val(meta), path("*cnn.vcf.gz.tbi"), emit: tbi
-    path "versions.yml"                     , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,52 +24,42 @@ process GATK4_CNNSCOREVARIANTS {
     script:
     // Exit if running this module with -profile conda / -profile mamba
     if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "GATK4_CNNSCOREVARIANTS module does not support Conda. Please use Docker / Singularity / Podman instead."
+        error("GATK4_CNNSCOREVARIANTS module does not support Conda. Please use Docker / Singularity / Podman instead.")
     }
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def aligned_input_cmd = aligned_input ? "--input $aligned_input" : ""
-    def interval_command = intervals ? "--intervals $intervals" : ""
-    def architecture_cmd = architecture ? "--architecture $architecture" : ""
-    def weights_cmd = weights ? "--weights $weights" : ""
+    def aligned_input_cmd = aligned_input ? "--input ${aligned_input}" : ""
+    def interval_command = intervals ? "--intervals ${intervals}" : ""
+    def architecture_cmd = architecture ? "--architecture ${architecture}" : ""
+    def weights_cmd = weights ? "--weights ${weights}" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK CnnScoreVariants] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK CnnScoreVariants] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     export THEANO_FLAGS="base_compiledir=\$PWD"
 
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         CNNScoreVariants \\
-        --variant $vcf \\
+        --variant ${vcf} \\
         --output ${prefix}.cnn.vcf.gz \\
-        --reference $fasta \\
-        $interval_command \\
-        $aligned_input_cmd \\
-        $architecture_cmd \\
-        $weights_cmd \\
+        --reference ${fasta} \\
+        ${interval_command} \\
+        ${aligned_input_cmd} \\
+        ${architecture_cmd} \\
+        ${weights_cmd} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-
     """
     echo "" | gzip -c > ${prefix}.cnn.vcf.gz
     touch ${prefix}.cnn.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/cnnscorevariants/meta.yml b/modules/nf-core/gatk4/cnnscorevariants/meta.yml
index b55c9d9995..e39e38f987 100644
--- a/modules/nf-core/gatk4/cnnscorevariants/meta.yml
+++ b/modules/nf-core/gatk4/cnnscorevariants/meta.yml
@@ -25,40 +25,51 @@ input:
         type: file
         description: VCF file
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
     - tbi:
         type: file
         description: VCF index file
         pattern: "*.vcf.gz.tbi"
+        ontologies: []
     - aligned_input:
         type: file
         description: BAM/CRAM file from alignment (optional)
         pattern: "*.{bam,cram}"
+        ontologies: []
     - intervals:
         type: file
         description: Bed file with the genomic regions included in the library (optional)
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
-  - - architecture:
-        type: file
-        description: Neural Net architecture configuration json file (optional)
-        pattern: "*.json"
-  - - weights:
-        type: file
-        description: Keras model HD5 file with neural net weights. (optional)
-        pattern: "*.hd5"
+        ontologies: []
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
+  - architecture:
+      type: file
+      description: Neural Net architecture configuration json file (optional)
+      pattern: "*.json"
+      ontologies:
+        - edam: http://edamontology.org/format_3464 # JSON
+  - weights:
+      type: file
+      description: Keras model HD5 file with neural net weights. (optional)
+      pattern: "*.hd5"
+      ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -67,8 +78,9 @@ output:
           type: file
           description: Annotated VCF file
           pattern: "*.vcf"
-  - tbi:
-      - meta:
+          ontologies: []
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -77,11 +89,28 @@ output:
           type: file
           description: VCF index file
           pattern: "*.vcf.gz.tbi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/gatk4/createsequencedictionary/environment.yml b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/environment.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/createsequencedictionary/main.nf b/modules/nf-core/gatk4/createsequencedictionary/main.nf
index 872648a5e9..98ad30ce01 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/main.nf
+++ b/modules/nf-core/gatk4/createsequencedictionary/main.nf
@@ -3,16 +3,16 @@ process GATK4_CREATESEQUENCEDICTIONARY {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data'
-        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
     tuple val(meta), path('*.dict'), emit: dict
-    path "versions.yml",             emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -34,20 +34,10 @@ process GATK4_CREATESEQUENCEDICTIONARY {
         --URI ${fasta} \\
         --TMP_DIR . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
     """
     touch ${fasta.baseName}.dict
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
index 72dced28c8..5fdfcc20a7 100644
--- a/modules/nf-core/gatk4/createsequencedictionary/meta.yml
+++ b/modules/nf-core/gatk4/createsequencedictionary/meta.yml
@@ -39,13 +39,27 @@ output:
           description: gatk dictionary file
           pattern: "*.{dict}"
           ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@maxulysse"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/estimatelibrarycomplexity/environment.yml b/modules/nf-core/gatk4/estimatelibrarycomplexity/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/estimatelibrarycomplexity/environment.yml
+++ b/modules/nf-core/gatk4/estimatelibrarycomplexity/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/estimatelibrarycomplexity/main.nf b/modules/nf-core/gatk4/estimatelibrarycomplexity/main.nf
index 9071279577..613e9eb1f8 100644
--- a/modules/nf-core/gatk4/estimatelibrarycomplexity/main.nf
+++ b/modules/nf-core/gatk4/estimatelibrarycomplexity/main.nf
@@ -1,21 +1,21 @@
 process GATK4_ESTIMATELIBRARYCOMPLEXITY {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input)
-    path  fasta
-    path  fai
-    path  dict
+    path fasta
+    path fai
+    path dict
 
     output:
     tuple val(meta), path('*.metrics'), emit: metrics
-    path "versions.yml"               , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,28 +23,24 @@ process GATK4_ESTIMATELIBRARYCOMPLEXITY {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_list = input.collect(){"--INPUT $it"}.join(" ")
+    def input_list = input.collect { bam -> "--INPUT ${bam}" }.join(" ")
     def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK EstimateLibraryComplexity] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK EstimateLibraryComplexity] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         EstimateLibraryComplexity \\
-        $input_list \\
+        ${input_list} \\
         --OUTPUT ${prefix}.metrics \\
-        $reference \\
+        ${reference} \\
         --TMP_DIR . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
@@ -52,10 +48,5 @@ process GATK4_ESTIMATELIBRARYCOMPLEXITY {
 
     """
     touch ${prefix}.metrics
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/estimatelibrarycomplexity/meta.yml b/modules/nf-core/gatk4/estimatelibrarycomplexity/meta.yml
index 4fb06a3a2e..554e79c617 100644
--- a/modules/nf-core/gatk4/estimatelibrarycomplexity/meta.yml
+++ b/modules/nf-core/gatk4/estimatelibrarycomplexity/meta.yml
@@ -24,21 +24,25 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
+        ontologies: []
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
 output:
-  - metrics:
-      - meta:
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -47,11 +51,28 @@ output:
           type: file
           description: File containing metrics on the input files
           pattern: "*.{metrics}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
   - "@maxulysse"
diff --git a/modules/nf-core/gatk4/filtermutectcalls/environment.yml b/modules/nf-core/gatk4/filtermutectcalls/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/filtermutectcalls/environment.yml
+++ b/modules/nf-core/gatk4/filtermutectcalls/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/filtermutectcalls/main.nf b/modules/nf-core/gatk4/filtermutectcalls/main.nf
index d3c5bb5ad7..726a6e5530 100644
--- a/modules/nf-core/gatk4/filtermutectcalls/main.nf
+++ b/modules/nf-core/gatk4/filtermutectcalls/main.nf
@@ -1,11 +1,11 @@
 process GATK4_FILTERMUTECTCALLS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(vcf), path(vcf_tbi), path(stats), path(orientationbias), path(segmentation), path(table), val(estimate)
@@ -14,10 +14,10 @@ process GATK4_FILTERMUTECTCALLS {
     tuple val(meta4), path(dict)
 
     output:
-    tuple val(meta), path("*.vcf.gz")            , emit: vcf
-    tuple val(meta), path("*.vcf.gz.tbi")        , emit: tbi
+    tuple val(meta), path("*.vcf.gz"), emit: vcf
+    tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi
     tuple val(meta), path("*.filteringStats.tsv"), emit: stats
-    path "versions.yml"                          , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,34 +26,30 @@ process GATK4_FILTERMUTECTCALLS {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
 
-    def orientationbias_command = orientationbias ? orientationbias.collect{"--orientation-bias-artifact-priors $it"}.join(' ') : ''
-    def segmentation_command    = segmentation    ? segmentation.collect{"--tumor-segmentation $it"}.join(' ')                  : ''
-    def estimate_command        = estimate        ? " --contamination-estimate ${estimate} "                                    : ''
-    def table_command           = table           ? table.collect{"--contamination-table $it"}.join(' ')                        : ''
+    def orientationbias_command = orientationbias ? orientationbias.collect { orientationbias_ -> "--orientation-bias-artifact-priors ${orientationbias_}" }.join(' ') : ''
+    def segmentation_command = segmentation ? segmentation.collect { segmentation_ -> "--tumor-segmentation ${segmentation_}" }.join(' ') : ''
+    def estimate_command = estimate ? " --contamination-estimate ${estimate} " : ''
+    def table_command = table ? table.collect { table_ -> "--contamination-table ${table_}" }.join(' ') : ''
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK FilterMutectCalls] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK FilterMutectCalls] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         FilterMutectCalls \\
-        --variant $vcf \\
+        --variant ${vcf} \\
         --output ${prefix}.vcf.gz \\
-        --reference $fasta \\
-        $orientationbias_command \\
-        $segmentation_command \\
-        $estimate_command \\
-        $table_command \\
+        --reference ${fasta} \\
+        ${orientationbias_command} \\
+        ${segmentation_command} \\
+        ${estimate_command} \\
+        ${table_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
@@ -62,10 +58,5 @@ process GATK4_FILTERMUTECTCALLS {
     echo "" | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
     touch ${prefix}.vcf.gz.filteringStats.tsv
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/filtermutectcalls/meta.yml b/modules/nf-core/gatk4/filtermutectcalls/meta.yml
index 9287277eb7..eb3e9baf48 100644
--- a/modules/nf-core/gatk4/filtermutectcalls/meta.yml
+++ b/modules/nf-core/gatk4/filtermutectcalls/meta.yml
@@ -27,28 +27,36 @@ input:
         type: file
         description: compressed vcf file of mutect2calls
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
     - vcf_tbi:
         type: file
         description: Tabix index of vcf file
         pattern: "*vcf.gz.tbi"
+        ontologies: []
     - stats:
         type: file
         description: Stats file that pairs with output vcf file
         pattern: "*vcf.gz.stats"
+        ontologies: []
     - orientationbias:
         type: file
         description: files containing artifact priors for input vcf. Optional input.
         pattern: "*.artifact-prior.tar.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
     - segmentation:
         type: file
         description: tables containing segmentation information for input vcf. Optional
           input.
         pattern: "*.segmentation.table"
+        ontologies: []
     - table:
         type: file
         description: table(s) containing contamination data for input vcf. Optional
           input, takes priority over estimate.
         pattern: "*.contamination.table"
+        ontologies: []
     - estimate:
         type: float
         description: estimation of contamination value as a double. Optional input,
@@ -62,6 +70,7 @@ input:
         type: file
         description: The reference fasta file
         pattern: "*.fasta"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -71,6 +80,7 @@ input:
         type: file
         description: Index of reference fasta file
         pattern: "*.fasta.fai"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -80,39 +90,67 @@ input:
         type: file
         description: GATK sequence dictionary
         pattern: "*.dict"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: file
           description: file containing filtered mutect2 calls.
           pattern: "*.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.vcf.gz":
           type: file
           description: file containing filtered mutect2 calls.
           pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
           type: file
-          description: tbi file that pairs with vcf.
-          pattern: "*.vcf.gz.tbi"
+          description: file containing filtered mutect2 calls.
+          pattern: "*.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.vcf.gz.tbi":
           type: file
           description: tbi file that pairs with vcf.
           pattern: "*.vcf.gz.tbi"
-  - stats:
-      - meta:
+          ontologies: []
+  stats:
+    - - meta:
           type: file
-          description: file containing statistics of the filtermutectcalls run.
-          pattern: "*.filteringStats.tsv"
+          description: file containing filtered mutect2 calls.
+          pattern: "*.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.filteringStats.tsv":
           type: file
           description: file containing statistics of the filtermutectcalls run.
           pattern: "*.filteringStats.tsv"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3475 # TSV
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
   - "@maxulysse"
diff --git a/modules/nf-core/gatk4/filtervarianttranches/environment.yml b/modules/nf-core/gatk4/filtervarianttranches/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/filtervarianttranches/environment.yml
+++ b/modules/nf-core/gatk4/filtervarianttranches/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/filtervarianttranches/main.nf b/modules/nf-core/gatk4/filtervarianttranches/main.nf
index c5249b7a05..7c31a4b534 100644
--- a/modules/nf-core/gatk4/filtervarianttranches/main.nf
+++ b/modules/nf-core/gatk4/filtervarianttranches/main.nf
@@ -1,11 +1,11 @@
 process GATK4_FILTERVARIANTTRANCHES {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(vcf), path(tbi), path(intervals)
@@ -15,11 +15,10 @@ process GATK4_FILTERVARIANTTRANCHES {
     path fai
     path dict
 
-
     output:
-    tuple val(meta), path("*.vcf.gz")    , emit: vcf
+    tuple val(meta), path("*.vcf.gz"), emit: vcf
     tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi
-    path "versions.yml"                  , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,27 +26,23 @@ process GATK4_FILTERVARIANTTRANCHES {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def resource_list = resources.collect{"--resource $it"}.join(' ')
+    def resource_list = resources.collect { resource -> "--resource ${resource}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK FilterVariantTranches] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK FilterVariantTranches] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         FilterVariantTranches \\
-        --variant $vcf \\
-        $resource_list \\
+        --variant ${vcf} \\
+        ${resource_list} \\
         --output ${prefix}.filtered.vcf.gz \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
@@ -56,10 +51,5 @@ process GATK4_FILTERVARIANTTRANCHES {
     """
     echo "" | gzip -c > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/filtervarianttranches/meta.yml b/modules/nf-core/gatk4/filtervarianttranches/meta.yml
index 398bbb07c1..39a64b435b 100644
--- a/modules/nf-core/gatk4/filtervarianttranches/meta.yml
+++ b/modules/nf-core/gatk4/filtervarianttranches/meta.yml
@@ -25,38 +25,45 @@ input:
         type: file
         description: a VCF file containing variants, must have info key:CNN_2D
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
     - tbi:
         type: file
         description: tbi file matching with -vcf
         pattern: "*.vcf.gz.tbi"
+        ontologies: []
     - intervals:
         type: file
         description: Intervals
-  - - resources:
-        type: list
-        description: resource A VCF containing known SNP and or INDEL sites. Can be
-          supplied as many times as necessary
-        pattern: "*.vcf.gz"
-  - - resources_index:
-        type: list
-        description: Index of resource VCF containing known SNP and or INDEL sites.
-          Can be supplied as many times as necessary
-        pattern: "*.vcf.gz"
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: ".dict"
+        ontologies: []
+  - resources:
+      type: list
+      description: resource A VCF containing known SNP and or INDEL sites. Can be supplied
+        as many times as necessary
+      pattern: "*.vcf.gz"
+  - resources_index:
+      type: list
+      description: Index of resource VCF containing known SNP and or INDEL sites. Can
+        be supplied as many times as necessary
+      pattern: "*.vcf.gz"
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: ".dict"
+      ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -65,8 +72,10 @@ output:
           type: file
           description: VCF file
           pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -75,11 +84,28 @@ output:
           type: file
           description: VCF index file
           pattern: "*.vcf.gz.tbi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/gatk4/gatherbqsrreports/environment.yml b/modules/nf-core/gatk4/gatherbqsrreports/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/gatherbqsrreports/environment.yml
+++ b/modules/nf-core/gatk4/gatherbqsrreports/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/gatherbqsrreports/main.nf b/modules/nf-core/gatk4/gatherbqsrreports/main.nf
index fdc5a2a723..5e4fe4f7f7 100644
--- a/modules/nf-core/gatk4/gatherbqsrreports/main.nf
+++ b/modules/nf-core/gatk4/gatherbqsrreports/main.nf
@@ -1,18 +1,18 @@
 process GATK4_GATHERBQSRREPORTS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(table)
 
     output:
     tuple val(meta), path("*.table"), emit: table
-    path "versions.yml"             , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -20,25 +20,27 @@ process GATK4_GATHERBQSRREPORTS {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_list = table.collect{"--input $it"}.join(' ')
+    def input_list = table.collect { table_ -> "--input ${table_}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK GatherBQSRReports] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK GatherBQSRReports] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         GatherBQSRReports \\
-        $input_list \\
+        ${input_list} \\
         --output ${prefix}.table \\
         --tmp-dir . \\
-        $args
+        ${args}
+    """
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.table
     """
 }
diff --git a/modules/nf-core/gatk4/gatherbqsrreports/meta.yml b/modules/nf-core/gatk4/gatherbqsrreports/meta.yml
index 587175b3a4..94af5bdda1 100644
--- a/modules/nf-core/gatk4/gatherbqsrreports/meta.yml
+++ b/modules/nf-core/gatk4/gatherbqsrreports/meta.yml
@@ -24,9 +24,10 @@ input:
         type: file
         description: File(s) containing BQSR table(s)
         pattern: "*.table"
+        ontologies: []
 output:
-  - table:
-      - meta:
+  table:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -35,11 +36,28 @@ output:
           type: file
           description: File containing joined BQSR table
           pattern: "*.table"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/gatk4/gatherpileupsummaries/environment.yml b/modules/nf-core/gatk4/gatherpileupsummaries/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/gatherpileupsummaries/environment.yml
+++ b/modules/nf-core/gatk4/gatherpileupsummaries/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/gatherpileupsummaries/main.nf b/modules/nf-core/gatk4/gatherpileupsummaries/main.nf
index af397a1a2d..8f7c704ed8 100644
--- a/modules/nf-core/gatk4/gatherpileupsummaries/main.nf
+++ b/modules/nf-core/gatk4/gatherpileupsummaries/main.nf
@@ -1,20 +1,19 @@
 process GATK4_GATHERPILEUPSUMMARIES {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
-
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(pileup)
-    path  dict
+    path dict
 
     output:
     tuple val(meta), path("*.pileups.table"), emit: table
-    path "versions.yml"                     , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -22,37 +21,28 @@ process GATK4_GATHERPILEUPSUMMARIES {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_list = pileup.collect{ "--I $it" }.join(' ')
+    def input_list = pileup.collect { pileup_ -> "--I ${pileup_}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK GatherPileupSummaries] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK GatherPileupSummaries] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         GatherPileupSummaries \\
-        $input_list \\
+        ${input_list} \\
         --O ${prefix}.pileups.table \\
-        --sequence-dictionary $dict \\
+        --sequence-dictionary ${dict} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.pileups.table
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/gatherpileupsummaries/meta.yml b/modules/nf-core/gatk4/gatherpileupsummaries/meta.yml
index d8b29d2100..2d2b2a80db 100644
--- a/modules/nf-core/gatk4/gatherpileupsummaries/meta.yml
+++ b/modules/nf-core/gatk4/gatherpileupsummaries/meta.yml
@@ -23,12 +23,14 @@ input:
         type: file
         description: Pileup files from gatk4/getpileupsummaries
         pattern: "*.pileups.table"
-  - - dict:
-        type: file
-        description: dictionary
+        ontologies: []
+  - dict:
+      type: file
+      description: dictionary
+      ontologies: []
 output:
-  - table:
-      - meta:
+  table:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -37,11 +39,28 @@ output:
           type: file
           description: pileup summaries table file
           pattern: "*.pileups.table"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
   - "@maxulysse"
diff --git a/modules/nf-core/gatk4/genomicsdbimport/environment.yml b/modules/nf-core/gatk4/genomicsdbimport/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/genomicsdbimport/environment.yml
+++ b/modules/nf-core/gatk4/genomicsdbimport/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/genomicsdbimport/main.nf b/modules/nf-core/gatk4/genomicsdbimport/main.nf
index 90f1200dc7..1b4d193859 100644
--- a/modules/nf-core/gatk4/genomicsdbimport/main.nf
+++ b/modules/nf-core/gatk4/genomicsdbimport/main.nf
@@ -1,33 +1,33 @@
 process GATK4_GENOMICSDBIMPORT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(vcf), path(tbi), path(interval_file), val(interval_value), path(wspace)
-    val   run_intlist
-    val   run_updatewspace
-    val   input_map
+    val run_intlist
+    val run_updatewspace
+    val input_map
 
     output:
-    tuple val(meta), path("$prefix")        , optional:true, emit: genomicsdb
-    tuple val(meta), path("$updated_db")    , optional:true, emit: updatedb
-    tuple val(meta), path("*.interval_list"), optional:true, emit: intervallist
-    path "versions.yml"                                    , emit: versions
+    tuple val(meta), path("${prefix}"), emit: genomicsdb, optional: true
+    tuple val(meta), path("${updated_db}"), emit: updatedb, optional: true
+    tuple val(meta), path("*.interval_list"), emit: intervallist, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
 
     // settings for running default create gendb mode
-    input_command = input_map ? "--sample-name-map ${vcf[0]}" : vcf.collect(){"--variant $it"}.join(' ')
+    input_command = input_map ? "--sample-name-map ${vcf[0]}" : vcf.collect { vcf_ -> "--variant ${vcf_}" }.join(' ')
 
     genomicsdb_command = "--genomicsdb-workspace-path ${prefix}"
     interval_command = interval_file ? "--intervals ${interval_file}" : "--intervals ${interval_value}"
@@ -48,27 +48,23 @@ process GATK4_GENOMICSDBIMPORT {
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK GenomicsDBImport] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK GenomicsDBImport] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         GenomicsDBImport \\
-        $input_command \\
-        $genomicsdb_command \\
-        $interval_command \\
+        ${input_command} \\
+        ${genomicsdb_command} \\
+        ${interval_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
 
     genomicsdb_command = "--genomicsdb-workspace-path ${prefix}"
     interval_command = interval_file ? "--intervals ${interval_file}" : "--intervals ${interval_value}"
@@ -88,17 +84,12 @@ process GATK4_GENOMICSDBIMPORT {
     }
 
     def stub_genomicsdb = genomicsdb_command == "--genomicsdb-workspace-path ${prefix}" ? "touch ${prefix}" : ""
-    def stub_interval   = interval_command == "--output-interval-list-to-file ${prefix}.interval_list" ? "touch ${prefix}.interval_list" : ""
-    def stub_update     = updated_db != "" ? "touch ${wspace}" : ""
+    def stub_interval = interval_command == "--output-interval-list-to-file ${prefix}.interval_list" ? "touch ${prefix}.interval_list" : ""
+    def stub_update = updated_db != "" ? "touch ${wspace}" : ""
 
     """
     ${stub_genomicsdb}
     ${stub_interval}
     ${stub_update}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/genomicsdbimport/meta.yml b/modules/nf-core/gatk4/genomicsdbimport/meta.yml
index ba734b288f..e1ed067698 100644
--- a/modules/nf-core/gatk4/genomicsdbimport/meta.yml
+++ b/modules/nf-core/gatk4/genomicsdbimport/meta.yml
@@ -36,6 +36,7 @@ input:
         type: file
         description: file containing the intervals to be used when creating the genomicsdb
         pattern: "*.interval_list"
+        ontologies: []
     - interval_value:
         type: string
         description: if an intervals file has not been specified, the value entered
@@ -47,68 +48,71 @@ input:
           get intervals mode. This WILL NOT specify name of a new genomicsdb in create
           db mode.
         pattern: "/path/to/existing/gendb"
-  - - run_intlist:
-        type: boolean
-        description: Specify whether to run get interval list mode, this option cannot
-          be specified at the same time as run_updatewspace.
-        pattern: "true/false"
-  - - run_updatewspace:
-        type: boolean
-        description: Specify whether to run update genomicsdb mode, this option takes
-          priority over run_intlist.
-        pattern: "true/false"
-  - - input_map:
-        type: boolean
-        description: Specify whether the vcf input is providing a list of vcf file(s)
-          or a single file containing a map of paths to vcf files to be used to create
-          or update a genomicsdb.
-        pattern: "*.sample_map"
+        ontologies: []
+  - run_intlist:
+      type: boolean
+      description: Specify whether to run get interval list mode, this option cannot
+        be specified at the same time as run_updatewspace.
+      pattern: "true/false"
+  - run_updatewspace:
+      type: boolean
+      description: Specify whether to run update genomicsdb mode, this option takes
+        priority over run_intlist.
+      pattern: "true/false"
+  - input_map:
+      type: boolean
+      description: Specify whether the vcf input is providing a list of vcf file(s)
+        or a single file containing a map of paths to vcf files to be used to create
+        or update a genomicsdb.
+      pattern: "*.sample_map"
 output:
-  - genomicsdb:
-      - meta:
-          type: directory
-          description: Directory containing the files that compose the genomicsdb workspace,
-            this is only output for create mode, as update changes an existing db
-          pattern: "*/$prefix"
-      - $prefix:
-          type: directory
-          description: Directory containing the files that compose the genomicsdb workspace,
-            this is only output for create mode, as update changes an existing db
-          pattern: "*/$prefix"
-  - updatedb:
-      - meta:
-          type: directory
-          description: Directory containing the files that compose the updated genomicsdb
-            workspace, this is only output for update mode, and should be the same path
-            as the input wspace.
-          pattern: "same/path/as/wspace"
-      - $updated_db:
-          type: directory
-          description: Directory containing the files that compose the updated genomicsdb
-            workspace, this is only output for update mode, and should be the same path
-            as the input wspace.
-          pattern: "same/path/as/wspace"
-  - intervallist:
-      - meta:
+  genomicsdb:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - ${prefix}:
           type: file
-          description: File containing the intervals used to generate the genomicsdb,
-            only created by get intervals mode.
-          pattern: "*.interval_list"
-      - "*.interval_list":
+          description: genomicsdb
+          ontologies: []
+  updatedb:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - ${updated_db}:
           type: file
-          description: File containing the intervals used to generate the genomicsdb,
-            only created by get intervals mode.
-          pattern: "*.interval_list"
-      - list:
+          description: updated genomicsdb
+          ontologies: []
+  intervallist:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - "*.interval_list":
           type: file
           description: File containing the intervals used to generate the genomicsdb,
             only created by get intervals mode.
           pattern: "*.interval_list"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
 maintainers:
diff --git a/modules/nf-core/gatk4/genotypegvcfs/environment.yml b/modules/nf-core/gatk4/genotypegvcfs/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/genotypegvcfs/environment.yml
+++ b/modules/nf-core/gatk4/genotypegvcfs/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/genotypegvcfs/main.nf b/modules/nf-core/gatk4/genotypegvcfs/main.nf
index dc2813a350..e90e1d529e 100644
--- a/modules/nf-core/gatk4/genotypegvcfs/main.nf
+++ b/modules/nf-core/gatk4/genotypegvcfs/main.nf
@@ -1,11 +1,11 @@
 process GATK4_GENOTYPEGVCFS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input), path(gvcf_index), path(intervals), path(intervals_index)
@@ -17,8 +17,8 @@ process GATK4_GENOTYPEGVCFS {
 
     output:
     tuple val(meta), path("*.vcf.gz"), emit: vcf
-    tuple val(meta), path("*.tbi")   , emit: tbi
-    path  "versions.yml"             , emit: versions
+    tuple val(meta), path("*.tbi"), emit: tbi
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,31 +26,27 @@ process GATK4_GENOTYPEGVCFS {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_command = input.name.endsWith(".vcf") || input.name.endsWith(".vcf.gz") ? "$input" : "gendb://$input"
-    def dbsnp_command = dbsnp ? "--dbsnp $dbsnp" : ""
-    def interval_command = intervals ? "--intervals $intervals" : ""
+    def input_command = input.name.endsWith(".vcf") || input.name.endsWith(".vcf.gz") ? "${input}" : "gendb://${input}"
+    def dbsnp_command = dbsnp ? "--dbsnp ${dbsnp}" : ""
+    def interval_command = intervals ? "--intervals ${intervals}" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK GenotypeGVCFs] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK GenotypeGVCFs] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         GenotypeGVCFs \\
-        --variant $input_command \\
+        --variant ${input_command} \\
         --output ${prefix}.vcf.gz \\
-        --reference $fasta \\
-        $interval_command \\
-        $dbsnp_command \\
+        --reference ${fasta} \\
+        ${interval_command} \\
+        ${dbsnp_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
@@ -59,10 +55,5 @@ process GATK4_GENOTYPEGVCFS {
     """
     echo | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/genotypegvcfs/meta.yml b/modules/nf-core/gatk4/genotypegvcfs/meta.yml
index 0c1fe491fe..9fb6179b45 100644
--- a/modules/nf-core/gatk4/genotypegvcfs/meta.yml
+++ b/modules/nf-core/gatk4/genotypegvcfs/meta.yml
@@ -26,18 +26,22 @@ input:
         description: |
           gVCF(.gz) file or a GenomicsDB
         pattern: "*.{vcf,vcf.gz}"
+        ontologies: []
     - gvcf_index:
         type: file
         description: |
           index of gvcf file, or empty when providing GenomicsDB
         pattern: "*.{idx,tbi}"
+        ontologies: []
     - intervals:
         type: file
         description: Interval file with the genomic regions included in the library
           (optional)
+        ontologies: []
     - intervals_index:
         type: file
         description: Interval index file (optional)
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -47,6 +51,7 @@ input:
         type: file
         description: Reference fasta file
         pattern: "*.fasta"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -56,6 +61,7 @@ input:
         type: file
         description: Reference fasta index file
         pattern: "*.fai"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -65,6 +71,7 @@ input:
         type: file
         description: Reference fasta sequence dict file
         pattern: "*.dict"
+        ontologies: []
   - - meta5:
         type: map
         description: |
@@ -74,6 +81,8 @@ input:
         type: file
         description: dbSNP VCF file
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
   - - meta6:
         type: map
         description: |
@@ -83,9 +92,10 @@ input:
         type: file
         description: dbSNP VCF index file
         pattern: "*.tbi"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -94,8 +104,10 @@ output:
           type: file
           description: Genotyped VCF file
           pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -104,11 +116,29 @@ output:
           type: file
           description: Tbi index for VCF file
           pattern: "*.vcf.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@santiagorevale"
   - "@maxulysse"
diff --git a/modules/nf-core/gatk4/getpileupsummaries/environment.yml b/modules/nf-core/gatk4/getpileupsummaries/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/getpileupsummaries/environment.yml
+++ b/modules/nf-core/gatk4/getpileupsummaries/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/getpileupsummaries/main.nf b/modules/nf-core/gatk4/getpileupsummaries/main.nf
index 41fd312811..c7f6d3308a 100644
--- a/modules/nf-core/gatk4/getpileupsummaries/main.nf
+++ b/modules/nf-core/gatk4/getpileupsummaries/main.nf
@@ -1,23 +1,23 @@
 process GATK4_GETPILEUPSUMMARIES {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input), path(index), path(intervals)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(fai)
     tuple val(meta4), path(dict)
-    path  variants
-    path  variants_tbi
+    path variants
+    path variants_tbi
 
     output:
     tuple val(meta), path('*.pileups.table'), emit: table
-    path "versions.yml"                     , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,40 +25,31 @@ process GATK4_GETPILEUPSUMMARIES {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def interval_command = intervals ? "--intervals $intervals" : "--intervals $variants"
-    def reference_command = fasta ? "--reference $fasta" : ''
+    def interval_command = intervals ? "--intervals ${intervals}" : "--intervals ${variants}"
+    def reference_command = fasta ? "--reference ${fasta}" : ''
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK GetPileupSummaries] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK GetPileupSummaries] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         GetPileupSummaries \\
-        --input $input \\
-        --variant $variants \\
+        --input ${input} \\
+        --variant ${variants} \\
         --output ${prefix}.pileups.table \\
-        $reference_command \\
-        $interval_command \\
+        ${reference_command} \\
+        ${interval_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.pileups.table
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/getpileupsummaries/meta.yml b/modules/nf-core/gatk4/getpileupsummaries/meta.yml
index 86b851e13a..04113b1101 100644
--- a/modules/nf-core/gatk4/getpileupsummaries/meta.yml
+++ b/modules/nf-core/gatk4/getpileupsummaries/meta.yml
@@ -27,15 +27,18 @@ input:
         type: file
         description: BAM/CRAM file to be summarised.
         pattern: "*.{bam,cram}"
+        ontologies: []
     - index:
         type: file
         description: Index file for the input BAM/CRAM file.
         pattern: "*.{bam.bai,cram.crai}"
+        ontologies: []
     - intervals:
         type: file
         description: File containing specified sites to be used for the summary. If
           this option is not specified, variants file is used instead automatically.
         pattern: "*.interval_list"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -45,6 +48,7 @@ input:
         type: file
         description: The reference fasta file
         pattern: "*.fasta"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -54,6 +58,7 @@ input:
         type: file
         description: Index of reference fasta file
         pattern: "*.fasta.fai"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -63,18 +68,22 @@ input:
         type: file
         description: GATK sequence dictionary
         pattern: "*.dict"
-  - - variants:
-        type: file
-        description: Population vcf of germline sequencing, containing allele fractions.
-          Is also used as sites file if no separate sites file is specified.
-        pattern: "*.vcf.gz"
-  - - variants_tbi:
-        type: file
-        description: Index file for the germline resource.
-        pattern: "*.vcf.gz.tbi"
+        ontologies: []
+  - variants:
+      type: file
+      description: Population vcf of germline sequencing, containing allele fractions.
+        Is also used as sites file if no separate sites file is specified.
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - variants_tbi:
+      type: file
+      description: Index file for the germline resource.
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
 output:
-  - table:
-      - meta:
+  table:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -83,11 +92,28 @@ output:
           type: file
           description: Table containing read counts for each site.
           pattern: "*.pileups.table"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
 maintainers:
diff --git a/modules/nf-core/gatk4/haplotypecaller/environment.yml b/modules/nf-core/gatk4/haplotypecaller/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/haplotypecaller/environment.yml
+++ b/modules/nf-core/gatk4/haplotypecaller/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/haplotypecaller/main.nf b/modules/nf-core/gatk4/haplotypecaller/main.nf
index 1ef76789de..33aed81fad 100644
--- a/modules/nf-core/gatk4/haplotypecaller/main.nf
+++ b/modules/nf-core/gatk4/haplotypecaller/main.nf
@@ -1,14 +1,14 @@
 process GATK4_HAPLOTYPECALLER {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
-    tuple val(meta),  path(input), path(input_index), path(intervals), path(dragstr_model)
+    tuple val(meta), path(input), path(input_index), path(intervals), path(dragstr_model)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(fai)
     tuple val(meta4), path(dict)
@@ -16,10 +16,10 @@ process GATK4_HAPLOTYPECALLER {
     tuple val(meta6), path(dbsnp_tbi)
 
     output:
-    tuple val(meta), path("*.vcf.gz")       , emit: vcf
-    tuple val(meta), path("*.tbi")          , optional:true, emit: tbi
-    tuple val(meta), path("*.realigned.bam"), optional:true, emit: bam
-    path "versions.yml"                     , emit: versions
+    tuple val(meta), path("*.vcf.gz"), emit: vcf
+    tuple val(meta), path("*.tbi"), emit: tbi, optional: true
+    tuple val(meta), path("*.realigned.bam"), emit: bam, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,35 +27,31 @@ process GATK4_HAPLOTYPECALLER {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def dbsnp_command = dbsnp ? "--dbsnp $dbsnp" : ""
-    def interval_command = intervals ? "--intervals $intervals" : ""
-    def dragstr_command = dragstr_model ? "--dragstr-params-path $dragstr_model" : ""
+    def dbsnp_command = dbsnp ? "--dbsnp ${dbsnp}" : ""
+    def interval_command = intervals ? "--intervals ${intervals}" : ""
+    def dragstr_command = dragstr_model ? "--dragstr-params-path ${dragstr_model}" : ""
     def bamout_command = args.contains("--bam-writer-type") ? "--bam-output ${prefix.replaceAll('.g\\s*$', '')}.realigned.bam" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         HaplotypeCaller \\
-        --input $input \\
+        --input ${input} \\
         --output ${prefix}.vcf.gz \\
-        --reference $fasta \\
+        --reference ${fasta} \\
         --native-pair-hmm-threads ${task.cpus} \\
-        $dbsnp_command \\
-        $interval_command \\
-        $dragstr_command \\
-        $bamout_command \\
+        ${dbsnp_command} \\
+        ${interval_command} \\
+        ${dragstr_command} \\
+        ${bamout_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
@@ -65,13 +61,8 @@ process GATK4_HAPLOTYPECALLER {
 
     def stub_realigned_bam = bamout_command ? "touch ${prefix.replaceAll('.g\\s*$', '')}.realigned.bam" : ""
     """
-    touch ${prefix}.vcf.gz
+    echo "" | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
     ${stub_realigned_bam}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/haplotypecaller/meta.yml b/modules/nf-core/gatk4/haplotypecaller/meta.yml
index 9d4a05e914..29a29bab8f 100644
--- a/modules/nf-core/gatk4/haplotypecaller/meta.yml
+++ b/modules/nf-core/gatk4/haplotypecaller/meta.yml
@@ -25,18 +25,22 @@ input:
         type: file
         description: BAM/CRAM file from alignment
         pattern: "*.{bam,cram}"
+        ontologies: []
     - input_index:
         type: file
         description: BAI/CRAI file from alignment
         pattern: "*.{bai,crai}"
+        ontologies: []
     - intervals:
         type: file
         description: Bed file with the genomic regions included in the library (optional)
+        ontologies: []
     - dragstr_model:
         type: file
         description: Text file containing the DragSTR model of the used BAM/CRAM file
           (optional)
         pattern: "*.txt"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -46,6 +50,7 @@ input:
         type: file
         description: The reference fasta file
         pattern: "*.fasta"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -55,6 +60,7 @@ input:
         type: file
         description: Index of reference fasta file
         pattern: "fasta.fai"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -64,6 +70,7 @@ input:
         type: file
         description: GATK sequence dictionary
         pattern: "*.dict"
+        ontologies: []
   - - meta5:
         type: map
         description: |
@@ -72,6 +79,7 @@ input:
     - dbsnp:
         type: file
         description: VCF file containing known sites (optional)
+        ontologies: []
   - - meta6:
         type: map
         description: |
@@ -80,9 +88,10 @@ input:
     - dbsnp_tbi:
         type: file
         description: VCF index of dbsnp (optional)
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,8 +100,10 @@ output:
           type: file
           description: Compressed VCF file
           pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -101,8 +112,9 @@ output:
           type: file
           description: Index of VCF file
           pattern: "*.vcf.gz.tbi"
-  - bam:
-      - meta:
+          ontologies: []
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -111,11 +123,28 @@ output:
           type: file
           description: Assembled haplotypes and locally realigned reads
           pattern: "*.realigned.bam"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@suzannejin"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4/intervallisttobed/environment.yml b/modules/nf-core/gatk4/intervallisttobed/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/intervallisttobed/environment.yml
+++ b/modules/nf-core/gatk4/intervallisttobed/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/intervallisttobed/main.nf b/modules/nf-core/gatk4/intervallisttobed/main.nf
index a7b05edbfc..94330b063e 100644
--- a/modules/nf-core/gatk4/intervallisttobed/main.nf
+++ b/modules/nf-core/gatk4/intervallisttobed/main.nf
@@ -3,16 +3,16 @@ process GATK4_INTERVALLISTTOBED {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data'
-        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(intervals)
 
     output:
     tuple val(meta), path("${prefix}.bed"), emit: bed
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -35,11 +35,6 @@ process GATK4_INTERVALLISTTOBED {
         --OUTPUT ${prefix}.bed \\
         --TMP_DIR . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -47,10 +42,5 @@ process GATK4_INTERVALLISTTOBED {
 
     """
     touch ${prefix}.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/intervallisttobed/meta.yml b/modules/nf-core/gatk4/intervallisttobed/meta.yml
index f151daafdb..316f66bf54 100644
--- a/modules/nf-core/gatk4/intervallisttobed/meta.yml
+++ b/modules/nf-core/gatk4/intervallisttobed/meta.yml
@@ -23,9 +23,10 @@ input:
     - intervals:
         type: file
         description: IntervalList file
+        ontologies: []
 output:
-  - bed:
-      - meta:
+  bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -36,11 +37,27 @@ output:
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
           pattern: "${prefix}.bed"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/gatk4/learnreadorientationmodel/environment.yml b/modules/nf-core/gatk4/learnreadorientationmodel/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/learnreadorientationmodel/environment.yml
+++ b/modules/nf-core/gatk4/learnreadorientationmodel/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/learnreadorientationmodel/main.nf b/modules/nf-core/gatk4/learnreadorientationmodel/main.nf
index 86e7daaa6c..500da54741 100644
--- a/modules/nf-core/gatk4/learnreadorientationmodel/main.nf
+++ b/modules/nf-core/gatk4/learnreadorientationmodel/main.nf
@@ -1,18 +1,18 @@
 process GATK4_LEARNREADORIENTATIONMODEL {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(f1r2)
 
     output:
     tuple val(meta), path("*.tar.gz"), emit: artifactprior
-    path "versions.yml"              , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -20,25 +20,27 @@ process GATK4_LEARNREADORIENTATIONMODEL {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_list = f1r2.collect{"--input $it"}.join(' ')
+    def input_list = f1r2.collect { f1r2_ -> "--input ${f1r2_}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK LearnReadOrientationModel] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK LearnReadOrientationModel] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         LearnReadOrientationModel \\
-        $input_list \\
+        ${input_list} \\
         --output ${prefix}.tar.gz \\
         --tmp-dir . \\
-        $args
+        ${args}
+    """
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    echo "" | gzip > ${prefix}.tar.gz
     """
 }
diff --git a/modules/nf-core/gatk4/learnreadorientationmodel/meta.yml b/modules/nf-core/gatk4/learnreadorientationmodel/meta.yml
index fde7829c8d..cf14f02a91 100644
--- a/modules/nf-core/gatk4/learnreadorientationmodel/meta.yml
+++ b/modules/nf-core/gatk4/learnreadorientationmodel/meta.yml
@@ -28,20 +28,40 @@ input:
         description: list of f1r2 files to be used as input.
         pattern: "*.f1r2.tar.gz"
 output:
-  - artifactprior:
-      - meta:
+  artifactprior:
+    - - meta:
           type: file
           description: file containing artifact-priors to be used by filtermutectcalls
           pattern: "*.tar.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
       - "*.tar.gz":
           type: file
           description: file containing artifact-priors to be used by filtermutectcalls
           pattern: "*.tar.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
 maintainers:
diff --git a/modules/nf-core/gatk4/markduplicates/environment.yml b/modules/nf-core/gatk4/markduplicates/environment.yml
index ca0fc1923a..4a13c61341 100644
--- a/modules/nf-core/gatk4/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4/markduplicates/environment.yml
@@ -6,7 +6,10 @@ channels:
 
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
+  # do not update - later htslib versions not compatible with gcnvkernel
   - bioconda::htslib=1.21
+  # do not update - later samtools versions not compatible with gcnvkernel
   - bioconda::samtools=1.21
diff --git a/modules/nf-core/gatk4/markduplicates/main.nf b/modules/nf-core/gatk4/markduplicates/main.nf
index f4bd896bf3..6fd44ea2ec 100644
--- a/modules/nf-core/gatk4/markduplicates/main.nf
+++ b/modules/nf-core/gatk4/markduplicates/main.nf
@@ -3,9 +3,9 @@ process GATK4_MARKDUPLICATES {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/927ff9bb80d65b425cbe752db6648a84043feff6e8ca90e60f9ff6ddbe8938d5/data'
-        : 'community.wave.seqera.io/library/gatk4_gcnvkernel_htslib_samtools:c1e4292d6ee27439'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e3/e3d753d93f57969fe76b8628a8dfcd23ef44bccd08c4ced7089c1f94bf47c89f/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel_htslib_samtools:d3becb6465454c35'}"
 
     input:
     tuple val(meta), path(bam)
@@ -18,7 +18,8 @@ process GATK4_MARKDUPLICATES {
     tuple val(meta), path("*.crai"), emit: crai, optional: true
     tuple val(meta), path("*.bai"), emit: bai, optional: true
     tuple val(meta), path("*.metrics"), emit: metrics
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,7 +31,7 @@ process GATK4_MARKDUPLICATES {
     // If the extension is CRAM, then change it to BAM
     prefix_bam = prefix.tokenize('.')[-1] == 'cram' ? "${prefix.substring(0, prefix.lastIndexOf('.'))}.bam" : prefix
 
-    def input_list = bam.collect { "--INPUT ${it}" }.join(' ')
+    def input_list = bam.collect { bam_ -> "--INPUT ${bam_}" }.join(' ')
     def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : ""
 
     def avail_mem = 3072
@@ -59,12 +60,6 @@ process GATK4_MARKDUPLICATES {
         rm ${prefix_bam}
         samtools index ${prefix}
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -76,11 +71,5 @@ process GATK4_MARKDUPLICATES {
     touch ${prefix_no_suffix}.cram.crai
     touch ${prefix_no_suffix}.bai
     touch ${prefix}.metrics
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/markduplicates/meta.yml b/modules/nf-core/gatk4/markduplicates/meta.yml
index 4772c5f39a..33dbc1a3cb 100644
--- a/modules/nf-core/gatk4/markduplicates/meta.yml
+++ b/modules/nf-core/gatk4/markduplicates/meta.yml
@@ -1,6 +1,7 @@
 name: gatk4_markduplicates
-description: This tool locates and tags duplicate reads in a BAM or SAM file, where
-  duplicate reads are defined as originating from a single fragment of DNA.
+description: This tool locates and tags duplicate reads in a BAM or SAM file,
+  where duplicate reads are defined as originating from a single fragment of
+  DNA.
 keywords:
   - bam
   - gatk4
@@ -8,10 +9,11 @@ keywords:
   - sort
 tools:
   - gatk4:
-      description: Developed in the Data Sciences Platform at the Broad Institute, the
-        toolkit offers a wide variety of tools with a primary focus on variant discovery
-        and genotyping. Its powerful processing engine and high-performance computing
-        features make it capable of taking on projects of any size.
+      description: Developed in the Data Sciences Platform at the Broad Institute,
+        the toolkit offers a wide variety of tools with a primary focus on variant
+        discovery and genotyping. Its powerful processing engine and
+        high-performance computing features make it capable of taking on projects
+        of any size.
       homepage: https://gatk.broadinstitute.org/hc/en-us
       documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard-
       tool_dev_url: https://github.com/broadinstitute/gatk
@@ -28,17 +30,20 @@ input:
         type: file
         description: Sorted BAM file
         pattern: "*.{bam}"
-  - - fasta:
-        type: file
-        description: Fasta file
-        pattern: "*.{fasta}"
-  - - fasta_fai:
-        type: file
-        description: Fasta index file
-        pattern: "*.{fai}"
+        ontologies: []
+  - fasta:
+      type: file
+      description: Fasta file
+      pattern: "*.{fasta}"
+      ontologies: []
+  - fasta_fai:
+      type: file
+      description: Fasta index file
+      pattern: "*.{fai}"
+      ontologies: []
 output:
-  - cram:
-      - meta:
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -47,8 +52,9 @@ output:
           type: file
           description: Marked duplicates CRAM file
           pattern: "*.{cram}"
-  - bam:
-      - meta:
+          ontologies: []
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -57,8 +63,9 @@ output:
           type: file
           description: Marked duplicates BAM file
           pattern: "*.{bam}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -67,8 +74,9 @@ output:
           type: file
           description: CRAM index file
           pattern: "*.{cram.crai}"
-  - bai:
-      - meta:
+          ontologies: []
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -77,8 +85,9 @@ output:
           type: file
           description: BAM index file
           pattern: "*.{bam.bai}"
-  - metrics:
-      - meta:
+          ontologies: []
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -87,11 +96,49 @@ output:
           type: file
           description: Duplicate metrics file generated by GATK
           pattern: "*.{metrics.txt}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@ajodeh-juma"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4/mergemutectstats/environment.yml b/modules/nf-core/gatk4/mergemutectstats/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/mergemutectstats/environment.yml
+++ b/modules/nf-core/gatk4/mergemutectstats/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/mergemutectstats/main.nf b/modules/nf-core/gatk4/mergemutectstats/main.nf
index e6ddc6994c..a1da0358c7 100644
--- a/modules/nf-core/gatk4/mergemutectstats/main.nf
+++ b/modules/nf-core/gatk4/mergemutectstats/main.nf
@@ -1,18 +1,18 @@
 process GATK4_MERGEMUTECTSTATS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(stats)
 
     output:
     tuple val(meta), path("*.vcf.gz.stats"), emit: stats
-    path "versions.yml"                    , emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -20,36 +20,27 @@ process GATK4_MERGEMUTECTSTATS {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_list = stats.collect{ "--stats ${it}"}.join(' ')
+    def input_list = stats.collect { stats_ -> "--stats ${stats_}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK MergeMutectStats] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK MergeMutectStats] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         MergeMutectStats \\
-        $input_list \\
+        ${input_list} \\
         --output ${prefix}.vcf.gz.stats \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.vcf.gz.stats
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/mergemutectstats/meta.yml b/modules/nf-core/gatk4/mergemutectstats/meta.yml
index 09c8a54720..fc941347b0 100644
--- a/modules/nf-core/gatk4/mergemutectstats/meta.yml
+++ b/modules/nf-core/gatk4/mergemutectstats/meta.yml
@@ -24,9 +24,10 @@ input:
         type: file
         description: Stats file
         pattern: "*.{stats}"
+        ontologies: []
 output:
-  - stats:
-      - meta:
+  stats:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -35,11 +36,28 @@ output:
           type: file
           description: Stats file
           pattern: "*.vcf.gz.stats"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/gatk4/mutect2/environment.yml b/modules/nf-core/gatk4/mutect2/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/mutect2/environment.yml
+++ b/modules/nf-core/gatk4/mutect2/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/mutect2/main.nf b/modules/nf-core/gatk4/mutect2/main.nf
index 756dfca942..2ad8dba757 100644
--- a/modules/nf-core/gatk4/mutect2/main.nf
+++ b/modules/nf-core/gatk4/mutect2/main.nf
@@ -1,75 +1,70 @@
 process GATK4_MUTECT2 {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(intervals)
     tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
+    tuple val(meta3), path(fai), path(gzi)
     tuple val(meta4), path(dict)
-    path(germline_resource)
-    path(germline_resource_tbi)
-    path(panel_of_normals)
-    path(panel_of_normals_tbi)
+    path alleles
+    path alleles_tbi
+    path germline_resource
+    path germline_resource_tbi
+    path panel_of_normals
+    path panel_of_normals_tbi
 
     output:
-    tuple val(meta), path("*.vcf.gz")     , emit: vcf
-    tuple val(meta), path("*.tbi")        , emit: tbi
-    tuple val(meta), path("*.stats")      , emit: stats
-    tuple val(meta), path("*.f1r2.tar.gz"), optional:true, emit: f1r2
-    path "versions.yml"                   , emit: versions
+    tuple val(meta), path("${prefix}.vcf.gz"), emit: vcf
+    tuple val(meta), path("${prefix}.vcf.gz.stats"), emit: stats
+    tuple val(meta), path("${prefix}.vcf.gz.tbi"), emit: tbi
+    tuple val(meta), path("${prefix}.f1r2.tar.gz"), emit: f1r2, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def inputs = input.collect{ "--input $it"}.join(" ")
-    def interval_command = intervals ? "--intervals $intervals" : ""
-    def pon_command = panel_of_normals ? "--panel-of-normals $panel_of_normals" : ""
-    def gr_command = germline_resource ? "--germline-resource $germline_resource" : ""
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def inputs = input.collect { vcf_ -> "--input ${vcf_}" }.join(" ")
+    def interval_command = intervals ? "--intervals ${intervals}" : ""
+    def pon_command = panel_of_normals ? "--panel-of-normals ${panel_of_normals}" : ""
+    def gr_command = germline_resource ? "--germline-resource ${germline_resource}" : ""
+    def a_command = alleles ? "--alleles ${alleles}" : ""
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK Mutect2] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK Mutect2] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         Mutect2 \\
-        $inputs \\
+        ${inputs} \\
         --output ${prefix}.vcf.gz \\
-        --reference $fasta \\
-        $pon_command \\
-        $gr_command \\
-        $interval_command \\
+        --reference ${fasta} \\
+        ${pon_command} \\
+        ${gr_command} \\
+        ${a_command} \\
+        ${interval_command} \\
         --tmp-dir . \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     echo "" | gzip > ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
     touch ${prefix}.vcf.gz.stats
     echo "" | gzip > ${prefix}.f1r2.tar.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/mutect2/meta.yml b/modules/nf-core/gatk4/mutect2/meta.yml
index 27fd63a243..cbcae28299 100644
--- a/modules/nf-core/gatk4/mutect2/meta.yml
+++ b/modules/nf-core/gatk4/mutect2/meta.yml
@@ -37,6 +37,7 @@ input:
         type: file
         description: Specify region the tools is run on.
         pattern: ".{bed,interval_list}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -45,7 +46,8 @@ input:
     - fasta:
         type: file
         description: The reference fasta file
-        pattern: "*.fasta"
+        pattern: "*.{fasta,fasta.gz}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -54,7 +56,13 @@ input:
     - fai:
         type: file
         description: Index of reference fasta file
-        pattern: "*.fasta.fai"
+        pattern: "*.{fasta.fai,fasta.fai.gz}"
+        ontologies: []
+    - gzi:
+        type: file
+        description: Index of bgzipped reference fasta file
+        pattern: "*.fasta.gz.gzi"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -64,66 +72,101 @@ input:
         type: file
         description: GATK sequence dictionary
         pattern: "*.dict"
-  - - germline_resource:
-        type: file
-        description: Population vcf of germline sequencing, containing allele fractions.
-        pattern: "*.vcf.gz"
-  - - germline_resource_tbi:
-        type: file
-        description: Index file for the germline resource.
-        pattern: "*.vcf.gz.tbi"
-  - - panel_of_normals:
-        type: file
-        description: vcf file to be used as a panel of normals.
-        pattern: "*.vcf.gz"
-  - - panel_of_normals_tbi:
-        type: file
-        description: Index for the panel of normals.
-        pattern: "*.vcf.gz.tbi"
+        ontologies: []
+  - alleles:
+      type: file
+      description: vcf file to be used to force-call alleles.
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - alleles_tbi:
+      type: file
+      description: Index file for alleles to be force-called.
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
+  - germline_resource:
+      type: file
+      description: Population vcf of germline sequencing, containing allele fractions.
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - germline_resource_tbi:
+      type: file
+      description: Index file for the germline resource.
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
+  - panel_of_normals:
+      type: file
+      description: vcf file to be used as a panel of normals.
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - panel_of_normals_tbi:
+      type: file
+      description: Index for the panel of normals.
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
 output:
-  - vcf:
-      - meta:
-          type: file
-          description: compressed vcf file
-          pattern: "*.vcf.gz"
-      - "*.vcf.gz":
+  vcf:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - "${prefix}.vcf.gz":
           type: file
           description: compressed vcf file
-          pattern: "*.vcf.gz"
-  - tbi:
-      - meta:
+          pattern: "${prefix}.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  tbi:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - "${prefix}.vcf.gz.tbi":
           type: file
           description: Index of vcf file
-          pattern: "*vcf.gz.tbi"
-      - "*.tbi":
-          type: file
-          description: Index of vcf file
-          pattern: "*vcf.gz.tbi"
-  - stats:
-      - meta:
-          type: file
-          description: Stats file that pairs with output vcf file
-          pattern: "*vcf.gz.stats"
-      - "*.stats":
+          pattern: "${prefix}.vcf.gz.tbi"
+          ontologies: []
+  stats:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - "${prefix}.vcf.gz.stats":
           type: file
           description: Stats file that pairs with output vcf file
-          pattern: "*vcf.gz.stats"
-  - f1r2:
-      - meta:
+          pattern: "${prefix}.vcf.gz.stats"
+          ontologies: []
+  f1r2:
+    - - meta:
+          type: map
+          description: A Groovy map containing sample information
+      - "${prefix}.f1r2.tar.gz":
           type: file
           description: file containing information to be passed to LearnReadOrientationModel
             (only outputted when tumor_normal_pair mode is run)
-          pattern: "*.f1r2.tar.gz"
-      - "*.f1r2.tar.gz":
-          type: file
-          description: file containing information to be passed to LearnReadOrientationModel
-            (only outputted when tumor_normal_pair mode is run)
-          pattern: "*.f1r2.tar.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          pattern: "${prefix}.f1r2.tar.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
   - "@ramprasadn"
diff --git a/modules/nf-core/gatk4/variantrecalibrator/environment.yml b/modules/nf-core/gatk4/variantrecalibrator/environment.yml
index b562b72c74..67e0eb860a 100644
--- a/modules/nf-core/gatk4/variantrecalibrator/environment.yml
+++ b/modules/nf-core/gatk4/variantrecalibrator/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/gatk4
-  - bioconda::gatk4=4.6.1.0
+  - bioconda::gatk4=4.6.2.0
+  # renovate: datasource=conda depName=bioconda/gcnvkernel
   - bioconda::gcnvkernel=0.9
diff --git a/modules/nf-core/gatk4/variantrecalibrator/main.nf b/modules/nf-core/gatk4/variantrecalibrator/main.nf
index 3c6048f4ba..385d7ba28f 100644
--- a/modules/nf-core/gatk4/variantrecalibrator/main.nf
+++ b/modules/nf-core/gatk4/variantrecalibrator/main.nf
@@ -1,27 +1,27 @@
 process GATK4_VARIANTRECALIBRATOR {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/b2/b28daf5d9bb2f0d129dcad1b7410e0dd8a9b087aaf3ec7ced929b1f57624ad98/data':
-        'community.wave.seqera.io/library/gatk4_gcnvkernel:e48d414933d188cd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ce/ced519873646379e287bc28738bdf88e975edd39a92e7bc6a34bccd37153d9d0/data'
+        : 'community.wave.seqera.io/library/gatk4_gcnvkernel:edb12e4f0bf02cd3'}"
 
     input:
-    tuple val(meta), path(vcf), path(tbi) // input vcf and tbi of variants to recalibrate
-    path resource_vcf   // resource vcf
-    path resource_tbi   // resource tbi
-    val labels          // string (or list of strings) containing dedicated resource labels already formatted with '--resource:' tag
-    path  fasta
-    path  fai
-    path  dict
+    tuple val(meta), path(vcf), path(tbi)
+    path resource_vcf
+    path resource_tbi
+    val labels
+    path fasta
+    path fai
+    path dict
 
     output:
-    tuple val(meta), path("*.recal")   , emit: recal
-    tuple val(meta), path("*.idx")     , emit: idx
+    tuple val(meta), path("*.recal"), emit: recal
+    tuple val(meta), path("*.idx"), emit: idx
     tuple val(meta), path("*.tranches"), emit: tranches
-    tuple val(meta), path("*plots.R")  , emit: plots, optional:true
-    path "versions.yml"                , emit: versions
+    tuple val(meta), path("*plots.R"), emit: plots, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -29,43 +29,34 @@ process GATK4_VARIANTRECALIBRATOR {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def reference_command = fasta ? "--reference $fasta " : ''
+    def reference_command = fasta ? "--reference ${fasta} " : ''
     def labels_command = labels.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
-        log.info '[GATK VariantRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
-    } else {
-        avail_mem = (task.memory.mega*0.8).intValue()
+        log.info('[GATK VariantRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.')
+    }
+    else {
+        avail_mem = (task.memory.mega * 0.8).intValue()
     }
     """
     gatk --java-options "-Xmx${avail_mem}M -XX:-UsePerfData" \\
         VariantRecalibrator \\
-        --variant $vcf \\
+        --variant ${vcf} \\
         --output ${prefix}.recal \\
         --tranches-file ${prefix}.tranches \\
-        $reference_command \\
+        ${reference_command} \\
         --tmp-dir . \\
-        $labels_command \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
+        ${labels_command} \\
+        ${args}
     """
 
     stub:
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.recal
     touch ${prefix}.idx
     touch ${prefix}.tranches
     touch ${prefix}plots.R
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4/variantrecalibrator/meta.yml b/modules/nf-core/gatk4/variantrecalibrator/meta.yml
index 72fcfd601c..275e3a4f8c 100644
--- a/modules/nf-core/gatk4/variantrecalibrator/meta.yml
+++ b/modules/nf-core/gatk4/variantrecalibrator/meta.yml
@@ -29,81 +29,114 @@ input:
         type: file
         description: input vcf file containing the variants to be recalibrated
         pattern: "*.vcf.gz"
+        ontologies:
+          - edam: http://edamontology.org/format_3989 # GZIP format
     - tbi:
         type: file
         description: tbi file matching with -vcf
         pattern: "*.vcf.gz.tbi"
-  - - resource_vcf:
-        type: file
-        description: all resource vcf files that are used with the corresponding '--resource'
-          label
-        pattern: "*.vcf.gz"
-  - - resource_tbi:
-        type: file
-        description: all resource tbi files that are used with the corresponding '--resource'
-          label
-        pattern: "*.vcf.gz.tbi"
-  - - labels:
-        type: string
-        description: necessary arguments for GATK VariantRecalibrator. Specified to
-          directly match the resources provided. More information can be found at
-          https://gatk.broadinstitute.org/hc/en-us/articles/5358906115227-VariantRecalibrator
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
+        ontologies: []
+  - resource_vcf:
+      type: file
+      description: all resource vcf files that are used with the corresponding '--resource'
+        label
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - resource_tbi:
+      type: file
+      description: all resource tbi files that are used with the corresponding '--resource'
+        label
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
+  - labels:
+      type: string
+      description: necessary arguments for GATK VariantRecalibrator. Specified to directly
+        match the resources provided. More information can be found at
+        https://gatk.broadinstitute.org/hc/en-us/articles/5358906115227-VariantRecalibrator
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
 output:
-  - recal:
-      - meta:
+  recal:
+    - - meta:
           type: file
           description: Output recal file used by ApplyVQSR
           pattern: "*.recal"
+          ontologies: []
       - "*.recal":
           type: file
           description: Output recal file used by ApplyVQSR
           pattern: "*.recal"
-  - idx:
-      - meta:
+          ontologies: []
+  idx:
+    - - meta:
           type: file
-          description: Index file for the recal output file
-          pattern: "*.idx"
+          description: Output recal file used by ApplyVQSR
+          pattern: "*.recal"
+          ontologies: []
       - "*.idx":
           type: file
           description: Index file for the recal output file
           pattern: "*.idx"
-  - tranches:
-      - meta:
+          ontologies: []
+  tranches:
+    - - meta:
           type: file
-          description: Output tranches file used by ApplyVQSR
-          pattern: "*.tranches"
+          description: Output recal file used by ApplyVQSR
+          pattern: "*.recal"
+          ontologies: []
       - "*.tranches":
           type: file
           description: Output tranches file used by ApplyVQSR
           pattern: "*.tranches"
-  - plots:
-      - meta:
+          ontologies: []
+  plots:
+    - - meta:
           type: file
-          description: Optional output rscript file to aid in visualization of the input
-            data and learned model.
-          pattern: "*plots.R"
+          description: Output recal file used by ApplyVQSR
+          pattern: "*.recal"
+          ontologies: []
       - "*plots.R":
           type: file
           description: Optional output rscript file to aid in visualization of the input
             data and learned model.
           pattern: "*plots.R"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3999 # R script
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@GCJMackenzie"
   - "@nickhsmith"
diff --git a/modules/nf-core/gatk4spark/applybqsr/environment.yml b/modules/nf-core/gatk4spark/applybqsr/environment.yml
index a5c49e9557..fa5743a55f 100644
--- a/modules/nf-core/gatk4spark/applybqsr/environment.yml
+++ b/modules/nf-core/gatk4spark/applybqsr/environment.yml
@@ -4,4 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::gatk4-spark=4.6.1.0
+  # renovate: datasource=conda depName=bioconda/gatk4-spark
+  - bioconda::gatk4-spark=4.6.2.0
diff --git a/modules/nf-core/gatk4spark/applybqsr/main.nf b/modules/nf-core/gatk4spark/applybqsr/main.nf
index 0c798f1ad7..4aec53545c 100644
--- a/modules/nf-core/gatk4spark/applybqsr/main.nf
+++ b/modules/nf-core/gatk4spark/applybqsr/main.nf
@@ -3,9 +3,9 @@ process GATK4SPARK_APPLYBQSR {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://depot.galaxyproject.org/singularity/gatk4-spark:4.6.1.0--hdfd78af_0'
-        : 'biocontainers/gatk4-spark:4.6.1.0--hdfd78af_0'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/49/498aea9c9bcaf736b9fb2a01366c1b7b38ccc0d38143178afc325d6a93241447/data'
+        : 'community.wave.seqera.io/library/gatk4-spark:4.6.2.0--8b5cd67ee60a714e'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals)
@@ -14,10 +14,10 @@ process GATK4SPARK_APPLYBQSR {
     path dict
 
     output:
-    tuple val(meta), path("${prefix}.bam"),  emit: bam,  optional: true
-    tuple val(meta), path("${prefix}*bai"),  emit: bai,  optional: true
+    tuple val(meta), path("${prefix}.bam"), emit: bam, optional: true
+    tuple val(meta), path("${prefix}*bai"), emit: bai, optional: true
     tuple val(meta), path("${prefix}.cram"), emit: cram, optional: true
-    path "versions.yml",                     emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -47,11 +47,6 @@ process GATK4SPARK_APPLYBQSR {
         --spark-master local[${task.cpus}] \\
         --tmp-dir . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -62,10 +57,5 @@ process GATK4SPARK_APPLYBQSR {
     if [[ ${suffix} == bam ]]; then
         touch ${prefix}.${suffix}.bai
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4spark/applybqsr/meta.yml b/modules/nf-core/gatk4spark/applybqsr/meta.yml
index da7bf56808..4d5c25821c 100644
--- a/modules/nf-core/gatk4spark/applybqsr/meta.yml
+++ b/modules/nf-core/gatk4spark/applybqsr/meta.yml
@@ -39,7 +39,8 @@ input:
         ontologies: []
     - intervals:
         type: file
-        description: Bed file with the genomic regions included in the library (optional)
+        description: Bed file with the genomic regions included in the library
+          (optional)
         ontologies: []
   - fasta:
       type: file
@@ -90,13 +91,29 @@ output:
           description: Recalibrated CRAM file
           pattern: "${prefix}.cram"
           ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@yocra3"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4spark/baserecalibrator/environment.yml b/modules/nf-core/gatk4spark/baserecalibrator/environment.yml
index a5c49e9557..fa5743a55f 100644
--- a/modules/nf-core/gatk4spark/baserecalibrator/environment.yml
+++ b/modules/nf-core/gatk4spark/baserecalibrator/environment.yml
@@ -4,4 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::gatk4-spark=4.6.1.0
+  # renovate: datasource=conda depName=bioconda/gatk4-spark
+  - bioconda::gatk4-spark=4.6.2.0
diff --git a/modules/nf-core/gatk4spark/baserecalibrator/main.nf b/modules/nf-core/gatk4spark/baserecalibrator/main.nf
index 1f9e2cb595..2b8deb171f 100644
--- a/modules/nf-core/gatk4spark/baserecalibrator/main.nf
+++ b/modules/nf-core/gatk4spark/baserecalibrator/main.nf
@@ -3,9 +3,9 @@ process GATK4SPARK_BASERECALIBRATOR {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://depot.galaxyproject.org/singularity/gatk4-spark:4.6.1.0--hdfd78af_0'
-        : 'biocontainers/gatk4-spark:4.6.1.0--hdfd78af_0'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/49/498aea9c9bcaf736b9fb2a01366c1b7b38ccc0d38143178afc325d6a93241447/data'
+        : 'community.wave.seqera.io/library/gatk4-spark:4.6.2.0--8b5cd67ee60a714e'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(intervals)
@@ -17,7 +17,7 @@ process GATK4SPARK_BASERECALIBRATOR {
 
     output:
     tuple val(meta), path("*.table"), emit: table
-    path "versions.yml",              emit: versions
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,7 +26,7 @@ process GATK4SPARK_BASERECALIBRATOR {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def interval_command = intervals ? "--intervals ${intervals}" : ""
-    def sites_command = known_sites.collect { "--known-sites ${it}" }.join(' ')
+    def sites_command = known_sites.collect { vcf -> "--known-sites ${vcf}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
@@ -46,11 +46,6 @@ process GATK4SPARK_BASERECALIBRATOR {
         --spark-master local[${task.cpus}] \\
         --tmp-dir . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -58,10 +53,5 @@ process GATK4SPARK_BASERECALIBRATOR {
 
     """
     touch ${prefix}.table
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4spark/baserecalibrator/meta.yml b/modules/nf-core/gatk4spark/baserecalibrator/meta.yml
index abb0e1a65e..e8e2a723b6 100644
--- a/modules/nf-core/gatk4spark/baserecalibrator/meta.yml
+++ b/modules/nf-core/gatk4spark/baserecalibrator/meta.yml
@@ -27,36 +27,45 @@ input:
         type: file
         description: BAM/CRAM file from alignment
         pattern: "*.{bam,cram}"
+        ontologies: []
     - input_index:
         type: file
         description: BAI/CRAI file from alignment
         pattern: "*.{bai,crai}"
+        ontologies: []
     - intervals:
         type: file
         description: Bed file with the genomic regions included in the library (optional)
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fasta.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
-  - - known_sites:
-        type: file
-        description: VCF files with known sites for indels / snps (optional)
-        pattern: "*.vcf.gz"
-  - - known_sites_tbi:
-        type: file
-        description: Tabix index of the known_sites (optional)
-        pattern: "*.vcf.gz.tbi"
+        ontologies: []
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fasta.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
+  - known_sites:
+      type: file
+      description: VCF files with known sites for indels / snps (optional)
+      pattern: "*.vcf.gz"
+      ontologies:
+        - edam: http://edamontology.org/format_3989 # GZIP format
+  - known_sites_tbi:
+      type: file
+      description: Tabix index of the known_sites (optional)
+      pattern: "*.vcf.gz.tbi"
+      ontologies: []
 output:
-  - table:
-      - meta:
+  table:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -65,11 +74,28 @@ output:
           type: file
           description: Recalibration table from BaseRecalibrator
           pattern: "*.{table}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@yocra3"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/gatk4spark/markduplicates/environment.yml b/modules/nf-core/gatk4spark/markduplicates/environment.yml
index a5c49e9557..fa5743a55f 100644
--- a/modules/nf-core/gatk4spark/markduplicates/environment.yml
+++ b/modules/nf-core/gatk4spark/markduplicates/environment.yml
@@ -4,4 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::gatk4-spark=4.6.1.0
+  # renovate: datasource=conda depName=bioconda/gatk4-spark
+  - bioconda::gatk4-spark=4.6.2.0
diff --git a/modules/nf-core/gatk4spark/markduplicates/main.nf b/modules/nf-core/gatk4spark/markduplicates/main.nf
index a0dcf3fee4..2278afa1ef 100644
--- a/modules/nf-core/gatk4spark/markduplicates/main.nf
+++ b/modules/nf-core/gatk4spark/markduplicates/main.nf
@@ -3,9 +3,9 @@ process GATK4SPARK_MARKDUPLICATES {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
-        ? 'https://depot.galaxyproject.org/singularity/gatk4-spark:4.6.1.0--hdfd78af_0'
-        : 'biocontainers/gatk4-spark:4.6.1.0--hdfd78af_0'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/49/498aea9c9bcaf736b9fb2a01366c1b7b38ccc0d38143178afc325d6a93241447/data'
+        : 'community.wave.seqera.io/library/gatk4-spark:4.6.2.0--8b5cd67ee60a714e'}"
 
     input:
     tuple val(meta), path(bam)
@@ -14,10 +14,10 @@ process GATK4SPARK_MARKDUPLICATES {
     path dict
 
     output:
-    tuple val(meta), path("${prefix}"),     emit: output
+    tuple val(meta), path("${prefix}"), emit: output
     tuple val(meta), path("${prefix}.bai"), emit: bam_index, optional: true
-    tuple val(meta), path("*.metrics"),     emit: metrics,   optional: true
-    path "versions.yml",                    emit: versions
+    tuple val(meta), path("*.metrics"), emit: metrics, optional: true
+    tuple val("${task.process}"), val('gatk4'), eval("gatk --version | sed -n '/GATK.*v/s/.*v//p'"), topic: versions, emit: versions_gatk4
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,7 +25,7 @@ process GATK4SPARK_MARKDUPLICATES {
     script:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}.bam"
-    def input_list = bam.collect { "--input ${it}" }.join(' ')
+    def input_list = bam.collect { bam_ -> "--input ${bam_}" }.join(' ')
 
     def avail_mem = 3072
     if (!task.memory) {
@@ -43,11 +43,6 @@ process GATK4SPARK_MARKDUPLICATES {
         --spark-master local[${task.cpus}] \\
         --tmp-dir . \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -56,10 +51,5 @@ process GATK4SPARK_MARKDUPLICATES {
     touch ${prefix}
     touch ${prefix}.bai
     touch ${prefix}.metrics
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gatk4spark/markduplicates/meta.yml b/modules/nf-core/gatk4spark/markduplicates/meta.yml
index fc8dee3dff..2f60373282 100644
--- a/modules/nf-core/gatk4spark/markduplicates/meta.yml
+++ b/modules/nf-core/gatk4spark/markduplicates/meta.yml
@@ -28,21 +28,25 @@ input:
         type: file
         description: Sorted BAM file
         pattern: "*.{bam}"
-  - - fasta:
-        type: file
-        description: The reference fasta file
-        pattern: "*.fasta"
-  - - fasta_fai:
-        type: file
-        description: Index of reference fasta file
-        pattern: "*.fai"
-  - - dict:
-        type: file
-        description: GATK sequence dictionary
-        pattern: "*.dict"
+        ontologies: []
+  - fasta:
+      type: file
+      description: The reference fasta file
+      pattern: "*.fasta"
+      ontologies: []
+  - fasta_fai:
+      type: file
+      description: Index of reference fasta file
+      pattern: "*.fai"
+      ontologies: []
+  - dict:
+      type: file
+      description: GATK sequence dictionary
+      pattern: "*.dict"
+      ontologies: []
 output:
-  - output:
-      - meta:
+  output:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,8 +55,9 @@ output:
           type: file
           description: Marked duplicates BAM/CRAM file
           pattern: "*.{bam,cram}"
-  - bam_index:
-      - meta:
+          ontologies: []
+  bam_index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -61,8 +66,9 @@ output:
           type: file
           description: Optional BAM index file
           pattern: "*.bai"
-  - metrics:
-      - meta:
+          ontologies: []
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,11 +77,28 @@ output:
           type: file
           description: Metrics file
           pattern: "*.metrics"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gatk4:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gatk4:
+          type: string
+          description: The name of the tool
+      - gatk --version | sed -n '/GATK.*v/s/.*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@ajodeh-juma"
   - "@FriederikeHanssen"
diff --git a/subworkflows/local/bam_applybqsr/main.nf b/subworkflows/local/bam_applybqsr/main.nf
index 597a7793cb..0814ed4e2c 100644
--- a/subworkflows/local/bam_applybqsr/main.nf
+++ b/subworkflows/local/bam_applybqsr/main.nf
@@ -57,7 +57,6 @@ workflow BAM_APPLYBQSR {
     // Gather versions of all tools used
     versions = versions.mix(BAM_MERGE_INDEX_SAMTOOLS.out.versions)
     versions = versions.mix(CRAM_MERGE_INDEX_SAMTOOLS.out.versions)
-    versions = versions.mix(GATK4_APPLYBQSR.out.versions)
 
     emit:
     alignment = recal_out // channel: [ meta, file, index ] — BAM or CRAM
diff --git a/subworkflows/local/bam_applybqsr_spark/main.nf b/subworkflows/local/bam_applybqsr_spark/main.nf
index 16e1e323ba..2948e97b04 100644
--- a/subworkflows/local/bam_applybqsr_spark/main.nf
+++ b/subworkflows/local/bam_applybqsr_spark/main.nf
@@ -57,7 +57,6 @@ workflow BAM_APPLYBQSR_SPARK {
     // Gather versions of all tools used
     versions = versions.mix(BAM_MERGE_INDEX_SAMTOOLS.out.versions)
     versions = versions.mix(CRAM_MERGE_INDEX_SAMTOOLS.out.versions)
-    versions = versions.mix(GATK4SPARK_APPLYBQSR.out.versions)
 
     emit:
     alignment = recal_out // channel: [ meta, file, index ] — BAM or CRAM
diff --git a/subworkflows/local/bam_baserecalibrator/main.nf b/subworkflows/local/bam_baserecalibrator/main.nf
index cc6f833a2d..e7461f4f96 100644
--- a/subworkflows/local/bam_baserecalibrator/main.nf
+++ b/subworkflows/local/bam_baserecalibrator/main.nf
@@ -18,7 +18,6 @@ workflow BAM_BASERECALIBRATOR {
     known_sites_tbi // channel: [optional]  [ known_sites_tbi ]
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -50,12 +49,8 @@ workflow BAM_BASERECALIBRATOR {
         // Remove no longer necessary field: num_intervals
         .map{ meta, table -> [ meta - meta.subMap('num_intervals'), table ] }
 
-    // Gather versions of all tools used
-    versions = versions.mix(GATK4_BASERECALIBRATOR.out.versions)
-    versions = versions.mix(GATK4_GATHERBQSRREPORTS.out.versions)
 
     emit:
     table_bqsr // channel: [ meta, table ]
 
-    versions   // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_baserecalibrator_spark/main.nf b/subworkflows/local/bam_baserecalibrator_spark/main.nf
index 39ff897bed..0ce20d7ae7 100644
--- a/subworkflows/local/bam_baserecalibrator_spark/main.nf
+++ b/subworkflows/local/bam_baserecalibrator_spark/main.nf
@@ -18,7 +18,6 @@ workflow BAM_BASERECALIBRATOR_SPARK {
     known_sites_tbi // channel: [optional]  [ known_sites_tbi ]
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -43,12 +42,8 @@ workflow BAM_BASERECALIBRATOR_SPARK {
         // Remove no longer necessary field: num_intervals
         .map{ meta, table -> [ meta - meta.subMap('num_intervals'), table ] }
 
-    // Gather versions of all tools used
-    versions = versions.mix(GATK4SPARK_BASERECALIBRATOR.out.versions)
-    versions = versions.mix(GATK4_GATHERBQSRREPORTS.out.versions)
 
     emit:
     table_bqsr // channel: [ meta, table ]
 
-    versions   // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
index a9973ba218..38badedd89 100644
--- a/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
+++ b/subworkflows/local/bam_joint_calling_germline_gatk/main.nf
@@ -31,7 +31,6 @@ workflow BAM_JOINT_CALLING_GERMLINE_GATK {
     known_snps_vqsr
 
     main:
-    versions = channel.empty()
 
     // Map input for GenomicsDBImport
     // Rename based on num_intervals, group all samples by their interval_name/interval_file and restructure for channel
@@ -147,14 +146,9 @@ workflow BAM_JOINT_CALLING_GERMLINE_GATK {
         [[id:"joint_variant_calling", patient:"all_samples", variantcaller:"haplotypecaller"], tbi_out]
     }
 
-    versions = versions.mix(GATK4_GENOMICSDBIMPORT.out.versions)
-    versions = versions.mix(GATK4_GENOTYPEGVCFS.out.versions)
-    versions = versions.mix(VARIANTRECALIBRATOR_SNP.out.versions)
-    versions = versions.mix(GATK4_APPLYVQSR_SNP.out.versions)
 
     emit:
     genotype_index  // channel: [ val(meta), [ tbi ] ]
     genotype_vcf    // channel: [ val(meta), [ vcf ] ]
 
-    versions        // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_markduplicates/main.nf b/subworkflows/local/bam_markduplicates/main.nf
index d3fb73f0f7..b1501ee40c 100644
--- a/subworkflows/local/bam_markduplicates/main.nf
+++ b/subworkflows/local/bam_markduplicates/main.nf
@@ -37,7 +37,6 @@ workflow BAM_MARKDUPLICATES {
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
     // Gather versions of all tools used
-    versions = versions.mix(GATK4_MARKDUPLICATES.out.versions)
     versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
 
     emit:
diff --git a/subworkflows/local/bam_markduplicates_spark/main.nf b/subworkflows/local/bam_markduplicates_spark/main.nf
index 96de5aef24..86a3eaadb8 100644
--- a/subworkflows/local/bam_markduplicates_spark/main.nf
+++ b/subworkflows/local/bam_markduplicates_spark/main.nf
@@ -42,8 +42,6 @@ workflow BAM_MARKDUPLICATES_SPARK {
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
     // Gather versions of all tools used
-    versions = versions.mix(GATK4_ESTIMATELIBRARYCOMPLEXITY.out.versions)
-    versions = versions.mix(GATK4SPARK_MARKDUPLICATES.out.versions)
     versions = versions.mix(INDEX_MARKDUPLICATES.out.versions)
     versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
 
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index d0ff46289a..d09184133a 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -153,7 +153,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         vcf_haplotypecaller = BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.vcf
         tbi_haplotypecaller = BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.tbi
 
-        versions = versions.mix(BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.versions)
 
         if (joint_germline) {
             BAM_JOINT_CALLING_GERMLINE_GATK(
@@ -173,7 +172,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
             vcf_haplotypecaller = BAM_JOINT_CALLING_GERMLINE_GATK.out.genotype_vcf
             tbi_haplotypecaller = BAM_JOINT_CALLING_GERMLINE_GATK.out.genotype_index
-            versions = versions.mix(BAM_JOINT_CALLING_GERMLINE_GATK.out.versions)
         } else {
 
             // If single sample track, check if filtering should be done
@@ -191,7 +189,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
                 vcf_haplotypecaller = VCF_VARIANT_FILTERING_GATK.out.filtered_vcf
                 tbi_haplotypecaller = VCF_VARIANT_FILTERING_GATK.out.filtered_tbi
 
-                versions = versions.mix(VCF_VARIANT_FILTERING_GATK.out.versions)
             }
         }
     }
@@ -337,8 +334,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
                 vcf_sentieon_haplotyper = SENTIEON_HAPLOTYPER_VCF_VARIANT_FILTERING_GATK.out.filtered_vcf
                 tbi_sentieon_haplotyper = SENTIEON_HAPLOTYPER_VCF_VARIANT_FILTERING_GATK.out.filtered_tbi
-
-                versions = versions.mix(SENTIEON_HAPLOTYPER_VCF_VARIANT_FILTERING_GATK.out.versions)
             }
         }
     }
diff --git a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
index a05ee92e4d..7ffda5a210 100644
--- a/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
+++ b/subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
@@ -19,7 +19,6 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
     intervals                    // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     vcf           = channel.empty()
     realigned_bam = channel.empty()
@@ -91,7 +90,6 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
 
     realigned_bam = BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai
 
-    versions = versions.mix(GATK4_HAPLOTYPECALLER.out.versions)
 
     // Remove no longer necessary field: num_intervals
     vcf = haplotypecaller_vcf.map{ meta, vcf_ -> [ meta - meta.subMap('num_intervals'), vcf_ ] }
@@ -103,5 +101,4 @@ workflow BAM_VARIANT_CALLING_HAPLOTYPECALLER {
     vcf                // vcf
     tbi                // tbi
 
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index e0027be4fc..b856eaac93 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -249,7 +249,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
         // vcf_mutect2 and tbi_mutect2 always contain usable output (filtered if available, otherwise unfiltered)
         vcf_mutect2 = BAM_VARIANT_CALLING_SOMATIC_MUTECT2.out.vcf
         tbi_mutect2 = BAM_VARIANT_CALLING_SOMATIC_MUTECT2.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_MUTECT2.out.versions)
     }
 
     // TNSCOPE
diff --git a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
index 8b65207486..f19610ec42 100644
--- a/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
@@ -28,7 +28,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     joint_mutect2         // boolean: [mandatory] [default: false] run mutect2 in joint mode
 
     main:
-    versions = channel.empty()
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
     // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
@@ -58,13 +57,13 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
             .combine(intervals)
             .map { meta, cram, crai, intervals_, num_intervals -> [meta + [num_intervals: num_intervals], cram, crai, intervals_] }
 
-        MUTECT2_PAIRED(ch_tn_intervals, fasta, fai, dict, germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
+        MUTECT2_PAIRED(ch_tn_intervals, fasta, fai.map { meta, index -> [ meta, index, [] ] }, dict, [], [], germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
     }
     else {
 
         // Perform variant calling using mutect2 module pair mode
         // meta: [id:tumor_id_vs_normal_id, normal_id, num_intervals, patient, sex, tumor_id]
-        MUTECT2_PAIRED(input_intervals, fasta, fai, dict, germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
+        MUTECT2_PAIRED(input_intervals, fasta, fai.map { meta, index -> [ meta, index, [] ] }, dict, [], [], germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
@@ -227,15 +226,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
         .concat(tbi.map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] })
         .unique { meta, _tbi -> meta }
 
-    versions = versions.mix(CALCULATECONTAMINATION.out.versions)
-    versions = versions.mix(FILTERMUTECTCALLS.out.versions)
-    versions = versions.mix(GETPILEUPSUMMARIES_NORMAL.out.versions)
-    versions = versions.mix(GETPILEUPSUMMARIES_TUMOR.out.versions)
-    versions = versions.mix(GATHERPILEUPSUMMARIES_NORMAL.out.versions)
-    versions = versions.mix(GATHERPILEUPSUMMARIES_TUMOR.out.versions)
-    versions = versions.mix(LEARNREADORIENTATIONMODEL.out.versions)
-    versions = versions.mix(MERGEMUTECTSTATS.out.versions)
-    versions = versions.mix(MUTECT2_PAIRED.out.versions)
 
     emit:
     vcf = vcf_mutect2   // channel: [ meta, vcf ] - filtered if germline_resource provided, otherwise unfiltered
@@ -247,5 +237,4 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MUTECT2 {
     pileup_table_tumor  // channel: [ meta, table_tumor ]
     contamination_table = calculatecontamination_out_cont // channel: [ meta, contamination ]
     segmentation_table  = calculatecontamination_out_seg // channel: [ meta, segmentation ]
-    versions            // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index e878d4dff9..91afd8a217 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -154,7 +154,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
         // vcf_mutect2 and tbi_mutect2 always contain usable output (filtered if available, otherwise unfiltered)
         vcf_mutect2 = BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2.out.vcf
         tbi_mutect2 = BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2.out.versions)
     }
 
     //LOFREQ
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
index b895920247..0c25712b97 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
@@ -27,7 +27,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     joint_mutect2         // boolean: [mandatory] [default: false] run mutect2 in joint mode
 
     main:
-    versions = channel.empty()
 
     // If no germline resource is provided, then create an empty channel to avoid GetPileupsummaries from being run
     // Handle channel.value([]) input from prepare_genome by converting to proper empty channel
@@ -50,11 +49,11 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
         input_joint_intervals = input_joint
             .combine(intervals)
             .map { meta, cram, crai, intervals_, num_intervals -> [meta + [num_intervals: num_intervals], cram, crai, intervals_] }
-        MUTECT2(input_joint_intervals, fasta, fai, dict, germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
+        MUTECT2(input_joint_intervals, fasta, fai.map { meta, index -> [ meta, index, [] ] }, dict, [], [], germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
     }
     else {
         // Perform variant calling using mutect2 module in tumor single mode
-        MUTECT2(input_intervals, fasta, fai, dict, germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
+        MUTECT2(input_intervals, fasta, fai.map { meta, index -> [ meta, index, [] ] }, dict, [], [], germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi)
     }
 
     // Figuring out if there is one or more vcf(s) from the same sample
@@ -164,13 +163,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
         .concat(tbi.map { meta, tbi_ -> [meta - meta.subMap('num_intervals') + [variantcaller: 'mutect2'], tbi_] })
         .unique { meta, _tbi -> meta }
 
-    versions = versions.mix(CALCULATECONTAMINATION.out.versions)
-    versions = versions.mix(FILTERMUTECTCALLS.out.versions)
-    versions = versions.mix(GETPILEUPSUMMARIES.out.versions)
-    versions = versions.mix(GATHERPILEUPSUMMARIES.out.versions)
-    versions = versions.mix(LEARNREADORIENTATIONMODEL.out.versions)
-    versions = versions.mix(MERGEMUTECTSTATS.out.versions)
-    versions = versions.mix(MUTECT2.out.versions)
 
     emit:
     vcf = vcf_mutect2   // channel: [ meta, vcf ] - filtered if germline_resource provided, otherwise unfiltered
@@ -185,5 +177,4 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MUTECT2 {
     contamination_table = calculatecontamination_out_cont  // channel: [ meta, contamination ]
     segmentation_table  = calculatecontamination_out_seg   // channel: [ meta, segmentation ]
 
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index f1ea54f64e..cbce80043e 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -404,7 +404,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 ch_table_bqsr_spark = BAM_BASERECALIBRATOR_SPARK.out.table_bqsr
 
                 // Gather used softwares versions
-                versions = versions.mix(BAM_BASERECALIBRATOR_SPARK.out.versions)
             } else {
 
             BAM_BASERECALIBRATOR(
@@ -419,7 +418,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 ch_table_bqsr_no_spark = BAM_BASERECALIBRATOR.out.table_bqsr
 
                 // Gather used softwares versions
-                versions = versions.mix(BAM_BASERECALIBRATOR.out.versions)
             }
 
             // ch_table_bqsr contains either:
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 3aa61c69fc..195355676e 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -93,7 +93,6 @@ workflow PREPARE_GENOME {
     if (!dict_in && step != "annotate") {
         GATK4_CREATESEQUENCEDICTIONARY(fasta)
         dict = GATK4_CREATESEQUENCEDICTIONARY.out.dict.collect()
-        versions = versions.mix(GATK4_CREATESEQUENCEDICTIONARY.out.versions)
     }
     else if (dict_in) {
         dict = channel.fromPath(dict_in).map { dict_ -> [[id: 'dict'], dict_] }.collect()
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index f6899250d0..6973896efb 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -61,7 +61,6 @@ workflow PREPARE_INTERVALS {
             if (intervals.endsWith(".interval_list")) {
                 GATK4_INTERVALLISTTOBED(intervals_combined)
                 intervals_combined = GATK4_INTERVALLISTTOBED.out.bed
-                versions = versions.mix(GATK4_INTERVALLISTTOBED.out.versions)
             }
         }
 
diff --git a/subworkflows/local/vcf_variant_filtering_gatk/main.nf b/subworkflows/local/vcf_variant_filtering_gatk/main.nf
index de98177777..769c5791ed 100644
--- a/subworkflows/local/vcf_variant_filtering_gatk/main.nf
+++ b/subworkflows/local/vcf_variant_filtering_gatk/main.nf
@@ -14,7 +14,6 @@ workflow VCF_VARIANT_FILTERING_GATK {
 
     main:
 
-    versions = channel.empty()
 
     // Don't scatter/gather by intervals, because especially for small regions (targeted or WGS), it easily fails with 0 SNPS in region
     cnn_in = vcf.combine(intervals_bed_combined).map{ meta, vcf_, tbi, intervals -> [ meta, vcf_, tbi, [], intervals ] }
@@ -31,12 +30,9 @@ workflow VCF_VARIANT_FILTERING_GATK {
         // remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals'), tbi ] }
 
-    versions = versions.mix(CNNSCOREVARIANTS.out.versions)
-    versions = versions.mix(FILTERVARIANTTRANCHES.out.versions)
 
     emit:
     filtered_vcf
     filtered_tbi
 
-    versions
 }
diff --git a/tests/aligner-bwa-mem.nf.test.snap b/tests/aligner-bwa-mem.nf.test.snap
index e1faa819eb..fb1f52fe06 100644
--- a/tests/aligner-bwa-mem.nf.test.snap
+++ b/tests/aligner-bwa-mem.nf.test.snap
@@ -17,7 +17,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -96,7 +96,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -147,7 +147,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -217,7 +217,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/aligner-bwa-mem2.nf.test.snap b/tests/aligner-bwa-mem2.nf.test.snap
index 5ca9778860..d03b12f392 100644
--- a/tests/aligner-bwa-mem2.nf.test.snap
+++ b/tests/aligner-bwa-mem2.nf.test.snap
@@ -10,7 +10,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -80,7 +80,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -159,7 +159,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -217,7 +217,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/aligner-dragmap.nf.test.snap b/tests/aligner-dragmap.nf.test.snap
index 93920ec70b..b59f4a72f0 100644
--- a/tests/aligner-dragmap.nf.test.snap
+++ b/tests/aligner-dragmap.nf.test.snap
@@ -10,7 +10,7 @@
                     "dragmap": "1.2.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -86,7 +86,7 @@
                     "dragmap": "1.2.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -168,7 +168,7 @@
                     "dragmap": "1.2.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -223,7 +223,7 @@
                     "dragmap": "1.2.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index 5f31c21071..1cd537c5ed 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -16,7 +16,7 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -246,7 +246,7 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -479,7 +479,7 @@
                     "bwa": "0.7.18-r1243-dirty"
                 },
                 "CALCULATECONTAMINATION": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -488,28 +488,28 @@
                     "fastqc": "0.12.1"
                 },
                 "FILTERMUTECTCALLS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GETPILEUPSUMMARIES_NORMAL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GETPILEUPSUMMARIES_TUMOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "PARABRICKS_FQ2BAM": {
                     "parabricks": "4.6.0-1"
diff --git a/tests/alignment_from_everything.nf.test.snap b/tests/alignment_from_everything.nf.test.snap
index 6ecda81125..a01f7e60cc 100644
--- a/tests/alignment_from_everything.nf.test.snap
+++ b/tests/alignment_from_everything.nf.test.snap
@@ -26,17 +26,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/alignment_to_fastq.nf.test.snap b/tests/alignment_to_fastq.nf.test.snap
index aa6286d3c1..605d3e424f 100644
--- a/tests/alignment_to_fastq.nf.test.snap
+++ b/tests/alignment_to_fastq.nf.test.snap
@@ -26,17 +26,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index ea7c990001..28b1cfe2b9 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -26,17 +26,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -390,17 +390,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -617,11 +617,11 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index 38bfde8a8b..4a3e6a3bf0 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -20,17 +20,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -347,17 +347,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/fastp.nf.test.snap b/tests/fastp.nf.test.snap
index 9730d00452..25e72cefa1 100644
--- a/tests/fastp.nf.test.snap
+++ b/tests/fastp.nf.test.snap
@@ -20,17 +20,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -383,17 +383,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -770,17 +770,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/joint_calling_haplotypecaller.nf.test.snap b/tests/joint_calling_haplotypecaller.nf.test.snap
index dd7b6e2735..964d5f4f53 100644
--- a/tests/joint_calling_haplotypecaller.nf.test.snap
+++ b/tests/joint_calling_haplotypecaller.nf.test.snap
@@ -13,13 +13,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_GENOMICSDBIMPORT": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_GENOTYPEGVCFS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_GENOTYPEGVCFS": {
                     "gatk4": "4.6.2.0"
@@ -262,13 +262,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_GENOMICSDBIMPORT": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_GENOTYPEGVCFS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_GENOTYPEGVCFS": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 6d94c5ade1..794d5c6ed3 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -10,16 +10,16 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -244,16 +244,16 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
diff --git a/tests/lane_integer.nf.test.snap b/tests/lane_integer.nf.test.snap
index 24300fc952..19bd1a0e5f 100644
--- a/tests/lane_integer.nf.test.snap
+++ b/tests/lane_integer.nf.test.snap
@@ -17,7 +17,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/multi_lane.nf.test.snap b/tests/multi_lane.nf.test.snap
index a9fa67c822..a9541143a4 100644
--- a/tests/multi_lane.nf.test.snap
+++ b/tests/multi_lane.nf.test.snap
@@ -17,17 +17,17 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -150,7 +150,7 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GROUPREADSBYUMI": {
                     "fgbio": "2.4.0"
diff --git a/tests/postprocess_concatenation.nf.test.snap b/tests/postprocess_concatenation.nf.test.snap
index 7c206ba449..0efeecd74f 100644
--- a/tests/postprocess_concatenation.nf.test.snap
+++ b/tests/postprocess_concatenation.nf.test.snap
@@ -19,7 +19,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
                     "bcftools": "1.23.1"
diff --git a/tests/postprocess_concatenation_normalization.nf.test.snap b/tests/postprocess_concatenation_normalization.nf.test.snap
index d0db0839ff..b37818373b 100644
--- a/tests/postprocess_concatenation_normalization.nf.test.snap
+++ b/tests/postprocess_concatenation_normalization.nf.test.snap
@@ -22,7 +22,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
                     "bcftools": "1.23.1"
@@ -350,7 +350,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GERMLINE_VCFS_CONCAT": {
                     "bcftools": "1.23.1"
diff --git a/tests/postprocess_consensus.nf.test.snap b/tests/postprocess_consensus.nf.test.snap
index b6c9b23f00..03d1848ea3 100644
--- a/tests/postprocess_consensus.nf.test.snap
+++ b/tests/postprocess_consensus.nf.test.snap
@@ -29,16 +29,16 @@
                     "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -395,10 +395,10 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LOFREQ": {
                     "lofreq": "2.1.5"
@@ -407,7 +407,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -702,7 +702,7 @@
                     "bcftools": "1.23.1"
                 },
                 "CALCULATECONTAMINATION": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "CONSENSUS_FROM_SITES": {
                     "gawk": "mawk 1.3.4 20240123",
@@ -712,25 +712,25 @@
                     "gawk": "5.3.0"
                 },
                 "FILTERMUTECTCALLS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GETPILEUPSUMMARIES_NORMAL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GETPILEUPSUMMARIES_TUMOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
diff --git a/tests/postprocess_filtering.nf.test.snap b/tests/postprocess_filtering.nf.test.snap
index bf295ba961..f31b3554e2 100644
--- a/tests/postprocess_filtering.nf.test.snap
+++ b/tests/postprocess_filtering.nf.test.snap
@@ -19,7 +19,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/postprocess_normalization.nf.test.snap b/tests/postprocess_normalization.nf.test.snap
index 8e019fffdd..2184783e7e 100644
--- a/tests/postprocess_normalization.nf.test.snap
+++ b/tests/postprocess_normalization.nf.test.snap
@@ -19,7 +19,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index 19239f404a..2cbbd34d14 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -25,7 +25,7 @@
                     "yte": "1.9.4"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_GERMLINE_SOMATIC_VCFS": {
                     "bcftools": "1.23.1"
@@ -330,7 +330,7 @@
                     "bcftools": "1.23.1"
                 },
                 "CALCULATECONTAMINATION": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "CONCAT_CALLED_CHUNKS": {
                     "bcftools": "1.23.1"
@@ -342,22 +342,22 @@
                     "yte": "1.9.4"
                 },
                 "FILTERMUTECTCALLS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATHERPILEUPSUMMARIES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GETPILEUPSUMMARIES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGEMUTECTSTATS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_MUTECT2": {
                     "gatk4": "4.6.2.0"
@@ -366,7 +366,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "RBT_VCFSPLIT": {
                     "rbt": "0.42.2"
@@ -599,7 +599,7 @@
                     "yte": "1.9.4"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_STRELKA": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/save_mapped.nf.test.snap b/tests/save_mapped.nf.test.snap
index 6c393f6a66..1c277f5804 100644
--- a/tests/save_mapped.nf.test.snap
+++ b/tests/save_mapped.nf.test.snap
@@ -17,7 +17,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 27a15e889b..52e6dde2b8 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -38,17 +38,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -387,7 +387,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -442,7 +442,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
diff --git a/tests/sentieon.nf.test.snap b/tests/sentieon.nf.test.snap
index 583a41a4fb..5d52871cb0 100644
--- a/tests/sentieon.nf.test.snap
+++ b/tests/sentieon.nf.test.snap
@@ -13,17 +13,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/sentieon_aligner_bwamem.nf.test.snap b/tests/sentieon_aligner_bwamem.nf.test.snap
index 04d1f56bde..4e05c5ff0c 100644
--- a/tests/sentieon_aligner_bwamem.nf.test.snap
+++ b/tests/sentieon_aligner_bwamem.nf.test.snap
@@ -13,7 +13,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -102,7 +102,7 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
@@ -173,7 +173,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/sentieon_dedup.nf.test.snap b/tests/sentieon_dedup.nf.test.snap
index 657289b852..4f48082aaf 100644
--- a/tests/sentieon_dedup.nf.test.snap
+++ b/tests/sentieon_dedup.nf.test.snap
@@ -7,13 +7,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -199,13 +199,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -391,13 +391,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -586,13 +586,13 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -781,13 +781,13 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/spark.nf.test.snap b/tests/spark.nf.test.snap
index b8d73e9355..9606f3e91b 100644
--- a/tests/spark.nf.test.snap
+++ b/tests/spark.nf.test.snap
@@ -17,19 +17,19 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4SPARK_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4SPARK_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4SPARK_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_ESTIMATELIBRARYCOMPLEXITY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -263,16 +263,16 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4SPARK_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4SPARK_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4SPARK_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/start_from_markduplicates.nf.test.snap b/tests/start_from_markduplicates.nf.test.snap
index 3f0c4293dd..dc2ee68e11 100644
--- a/tests/start_from_markduplicates.nf.test.snap
+++ b/tests/start_from_markduplicates.nf.test.snap
@@ -7,17 +7,17 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -194,17 +194,17 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -381,13 +381,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -538,13 +538,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/start_from_preparerecalibration.nf.test.snap b/tests/start_from_preparerecalibration.nf.test.snap
index c7260b1f8f..45e514d232 100644
--- a/tests/start_from_preparerecalibration.nf.test.snap
+++ b/tests/start_from_preparerecalibration.nf.test.snap
@@ -7,13 +7,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -157,7 +157,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -285,7 +285,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -410,13 +410,13 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/start_from_recalibration.nf.test.snap b/tests/start_from_recalibration.nf.test.snap
index 4b02d0f806..aa77cbc709 100644
--- a/tests/start_from_recalibration.nf.test.snap
+++ b/tests/start_from_recalibration.nf.test.snap
@@ -7,10 +7,10 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -137,7 +137,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -265,7 +265,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -390,10 +390,10 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 2c5e8d6bba..89c1068628 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -20,17 +20,17 @@
                     "fastqc": "0.12.1"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/umi_fastp.nf.test.snap b/tests/umi_fastp.nf.test.snap
index 42b59f49a1..54e35ad2af 100644
--- a/tests/umi_fastp.nf.test.snap
+++ b/tests/umi_fastp.nf.test.snap
@@ -23,17 +23,17 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/umi_fgbio.nf.test.snap b/tests/umi_fgbio.nf.test.snap
index ba01c82fd2..2504ee455e 100644
--- a/tests/umi_fgbio.nf.test.snap
+++ b/tests/umi_fgbio.nf.test.snap
@@ -55,17 +55,17 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "GROUPREADSBYUMI": {
                     "fgbio": "2.4.0"
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index dad1469bb4..a61ced6604 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -13,17 +13,17 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -294,17 +294,17 @@
                     "fgbio": "2.4.0"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index 7a0c71fea2..33a2044c8f 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -53,17 +53,17 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -694,17 +694,17 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -1155,7 +1155,7 @@
                     "samtools": 1.21
                 },
                 "CALCULATECONTAMINATION": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "CNVKIT_ANTITARGET": {
                     "cnvkit": "0.9.11"
@@ -1176,38 +1176,38 @@
                     "fastqc": "0.12.1"
                 },
                 "FILTERMUTECTCALLS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "FREEBAYES": {
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "GETPILEUPSUMMARIES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
diff --git a/tests/variant_calling_deepvariant.nf.test.snap b/tests/variant_calling_deepvariant.nf.test.snap
index 916214ce27..8e01a6b047 100644
--- a/tests/variant_calling_deepvariant.nf.test.snap
+++ b/tests/variant_calling_deepvariant.nf.test.snap
@@ -13,7 +13,7 @@
                     "deepvariant": "1.10.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -198,7 +198,7 @@
                     "deepvariant": "1.10.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 0b09f6ebd9..683a3576c9 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -16,7 +16,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_FREEBAYES": {
                     "gatk4": "4.6.2.0"
@@ -234,14 +234,14 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -642,17 +642,17 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_GATHERBQSRREPORTS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -1008,14 +1008,14 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -1349,7 +1349,7 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -1563,17 +1563,17 @@
                     "freebayes": "1.3.10"
                 },
                 "GATK4_APPLYBQSR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_BASERECALIBRATOR": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_GATHERBQSRREPORTS": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_MARKDUPLICATES": {
-                    "gatk4": "4.6.1.0",
-                    "samtools": 1.21
+                    "gatk4": "4.6.2.0",
+                    "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
diff --git a/tests/variant_calling_haplotypecaller.nf.test.snap b/tests/variant_calling_haplotypecaller.nf.test.snap
index fefcdc20f0..3d2db0137f 100644
--- a/tests/variant_calling_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_haplotypecaller.nf.test.snap
@@ -7,7 +7,7 @@
                     "bcftools": "1.23.1"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -187,7 +187,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_HAPLOTYPECALLER": {
                     "gatk4": "4.6.2.0"
@@ -377,10 +377,10 @@
                     "gatk4": "4.5.0.0"
                 },
                 "FILTERVARIANTTRANCHES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -566,10 +566,10 @@
                     "gawk": "5.3.0"
                 },
                 "FILTERVARIANTTRANCHES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_HAPLOTYPECALLER": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_HAPLOTYPECALLER": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/variant_calling_lofreq.nf.test.snap b/tests/variant_calling_lofreq.nf.test.snap
index 8e641e9b68..57807564f3 100644
--- a/tests/variant_calling_lofreq.nf.test.snap
+++ b/tests/variant_calling_lofreq.nf.test.snap
@@ -10,7 +10,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LOFREQ": {
                     "lofreq": "2.1.5"
@@ -192,7 +192,7 @@
                     "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LOFREQ": {
                     "lofreq": "2.1.5"
diff --git a/tests/variant_calling_msisensor2.nf.test.snap b/tests/variant_calling_msisensor2.nf.test.snap
index 6ef7a54f47..19d0e935d5 100644
--- a/tests/variant_calling_msisensor2.nf.test.snap
+++ b/tests/variant_calling_msisensor2.nf.test.snap
@@ -4,7 +4,7 @@
             4,
             {
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -97,7 +97,7 @@
             4,
             {
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -164,7 +164,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/variant_calling_msisensorpro.nf.test.snap b/tests/variant_calling_msisensorpro.nf.test.snap
index bb7a07206e..82da407438 100644
--- a/tests/variant_calling_msisensorpro.nf.test.snap
+++ b/tests/variant_calling_msisensorpro.nf.test.snap
@@ -4,7 +4,7 @@
             4,
             {
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MSISENSORPRO_SCAN": {
                     "msisensor-pro": "1.3.0"
@@ -53,7 +53,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -199,7 +199,7 @@
             4,
             {
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MSISENSORPRO_SCAN": {
                     "msisensor-pro": "1.3.0"
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index 978156f8df..7bb29f3633 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -13,7 +13,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -187,7 +187,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index 1046b8b134..57a9c11a95 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -7,16 +7,16 @@
                     "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -197,16 +197,16 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -408,16 +408,16 @@
                     "bcftools": "1.23.1"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2_PAIRED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
@@ -612,16 +612,16 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_CREATESEQUENCEDICTIONARY": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "LEARNREADORIENTATIONMODEL": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "MUTECT2": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": 1.21
diff --git a/tests/variant_calling_sentieon_dnascope.nf.test.snap b/tests/variant_calling_sentieon_dnascope.nf.test.snap
index 4b4aa56f68..4de48c9aaa 100644
--- a/tests/variant_calling_sentieon_dnascope.nf.test.snap
+++ b/tests/variant_calling_sentieon_dnascope.nf.test.snap
@@ -10,7 +10,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -196,7 +196,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -391,7 +391,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_GENOTYPEGVCFS": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
index 972acd6171..9375b5cee3 100644
--- a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
@@ -13,7 +13,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MERGE_GENOTYPEGVCFS": {
                     "gatk4": "4.6.2.0"
@@ -226,7 +226,7 @@
                     "gatk4": "4.5.0.0"
                 },
                 "FILTERVARIANTTRANCHES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -414,7 +414,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
@@ -603,10 +603,10 @@
                     "gawk": "5.3.0"
                 },
                 "FILTERVARIANTTRANCHES": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
diff --git a/tests/variant_calling_sentieon_tnscope.nf.test.snap b/tests/variant_calling_sentieon_tnscope.nf.test.snap
index 2c01eab0c2..7278a9d721 100644
--- a/tests/variant_calling_sentieon_tnscope.nf.test.snap
+++ b/tests/variant_calling_sentieon_tnscope.nf.test.snap
@@ -10,7 +10,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
@@ -97,7 +97,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
@@ -298,7 +298,7 @@
                     "gawk": "5.3.0"
                 },
                 "GATK4_INTERVALLISTTOBED": {
-                    "gatk4": "4.6.1.0"
+                    "gatk4": "4.6.2.0"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",

From e952f4ef56d2a9085989571f8a70b16b73527262 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Wed, 22 Jul 2026 21:03:36 +0200
Subject: [PATCH 15/27] chore(modules): migrate alignment/UMI/utility modules
 to versions topic channel (#2239)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates alignment, UMI and utility modules to the `versions` topic
channel. **Stacked on #2238** (base: `topic/gatk`).

### Changes
- Updates `bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`,
`gunzip`, `untar`, `unzip`, `tabix`, `spring` to their topic-channel
versions.
- Removes the corresponding `.out.versions` wiring from subworkflows.
- Fixes the `FASTP` call site for the new input signature (adapter fasta
folded into the reads tuple).
- Tool bumps ride along (see changelog) — notably **fastp 0.24.0 →
1.1.0** (major) and **fgbio 2.4.0 → 3.1.2**.

Test snapshots need regenerating in CI.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   7 +
 docs/output.md                                |   4 +-
 modules.json                                  |  36 +++---
 modules/nf-core/bwa/index/environment.yml     |   6 +-
 modules/nf-core/bwa/index/main.nf             |  23 +---
 modules/nf-core/bwa/index/meta.yml            |  27 +++-
 modules/nf-core/bwa/mem/environment.yml       |   6 +-
 modules/nf-core/bwa/mem/main.nf               |  33 +++--
 modules/nf-core/bwa/mem/meta.yml              | 112 +++++++++++-----
 modules/nf-core/bwamem2/index/environment.yml |   6 +-
 modules/nf-core/bwamem2/index/main.nf         |  20 +--
 modules/nf-core/bwamem2/index/meta.yml        |  49 +++++--
 modules/nf-core/bwamem2/mem/environment.yml   |   6 +-
 modules/nf-core/bwamem2/mem/main.nf           |  63 ++++-----
 modules/nf-core/bwamem2/mem/meta.yml          | 105 ++++++++++-----
 modules/nf-core/cat/cat/environment.yml       |   2 +-
 modules/nf-core/cat/cat/main.nf               |  76 ++++-------
 modules/nf-core/cat/cat/meta.yml              |  43 ++++--
 modules/nf-core/cat/fastq/main.nf             |  35 ++---
 modules/nf-core/cat/fastq/meta.yml            |  38 ++++--
 modules/nf-core/dragmap/align/main.nf         |  24 +---
 modules/nf-core/dragmap/align/meta.yml        | 119 +++++++++++++----
 modules/nf-core/dragmap/hashtable/main.nf     |  16 +--
 modules/nf-core/dragmap/hashtable/meta.yml    |  38 ++++--
 modules/nf-core/fastp/environment.yml         |   3 +-
 modules/nf-core/fastp/main.nf                 |  55 +++-----
 modules/nf-core/fastp/meta.yml                |  90 ++++++++-----
 .../environment.yml                           |   2 +-
 .../fgbio/callmolecularconsensusreads/main.nf |  38 +++---
 .../callmolecularconsensusreads/meta.yml      |  51 +++++---
 .../fgbio/copyumifromreadname/environment.yml |   2 +-
 .../nf-core/fgbio/copyumifromreadname/main.nf |  31 ++---
 .../fgbio/copyumifromreadname/meta.yml        |  35 +++--
 .../nf-core/fgbio/fastqtobam/environment.yml  |   2 +-
 modules/nf-core/fgbio/fastqtobam/main.nf      |  33 ++---
 modules/nf-core/fgbio/fastqtobam/meta.yml     |  54 ++++----
 .../fgbio/groupreadsbyumi/environment.yml     |   2 +-
 modules/nf-core/fgbio/groupreadsbyumi/main.nf |  51 ++++----
 .../nf-core/fgbio/groupreadsbyumi/meta.yml    |  59 +++++++--
 modules/nf-core/gawk/environment.yml          |   2 +-
 modules/nf-core/gawk/main.nf                  |  32 ++---
 modules/nf-core/gawk/meta.yml                 |  69 ++++++----
 modules/nf-core/gunzip/main.nf                |  24 ++--
 modules/nf-core/gunzip/meta.yml               |  35 ++++-
 modules/nf-core/spring/decompress/main.nf     |  22 +---
 modules/nf-core/spring/decompress/meta.yml    |  42 ++++--
 modules/nf-core/untar/main.nf                 |  13 +-
 modules/nf-core/untar/meta.yml                |  36 ++++--
 modules/nf-core/unzip/main.nf                 |  17 +--
 modules/nf-core/unzip/meta.yml                |  35 +++--
 .../local/bam_convert_samtools/main.nf        |   1 -
 .../local/bam_variant_calling_mpileup/main.nf |   1 -
 subworkflows/local/fastq_align/main.nf        |   6 -
 .../fastq_create_umi_consensus_fgbio/main.nf  |   4 -
 .../local/fastq_preprocess_gatk/main.nf       |   8 +-
 subworkflows/local/prepare_genome/main.nf     |  10 --
 subworkflows/local/prepare_intervals/main.nf  |   1 -
 tests/aligner-bwa-mem.nf.test.snap            |  16 +--
 tests/aligner-bwa-mem2.nf.test.snap           |   4 +-
 tests/aligner-parabricks.nf.test.snap         |   8 +-
 tests/alignment_from_everything.nf.test.snap  |   8 +-
 tests/alignment_to_fastq.nf.test.snap         |   8 +-
 tests/bbsplit.nf.test.snap                    |  20 +--
 tests/default.nf.test.snap                    |  12 +-
 tests/fastp.nf.test.snap                      | 122 +++++++++---------
 tests/intervals.nf.test.snap                  |  40 +++---
 tests/joint_calling_mutect2.nf.test.snap      |   2 +-
 tests/lane_integer.nf.test.snap               |   6 +-
 tests/multi_lane.nf.test.snap                 |  30 +++--
 tests/save_mapped.nf.test.snap                |   6 +-
 tests/save_output_as_bam.nf.test.snap         |  12 +-
 tests/sentieon.nf.test.snap                   |   2 +-
 tests/sentieon_aligner_bwamem.nf.test.snap    |  12 +-
 tests/sentieon_dedup.nf.test.snap             |   4 +-
 tests/spark.nf.test.snap                      |  12 +-
 tests/tumor-normal-pair.nf.test.snap          |   6 +-
 tests/umi_fastp.nf.test.snap                  |  14 +-
 tests/umi_fgbio.nf.test.snap                  |  58 +++++----
 tests/umi_in_read_names.nf.test.snap          |  12 +-
 tests/variant_calling_all.nf.test.snap        |  18 +--
 .../variant_calling_controlfreec.nf.test.snap |   8 +-
 tests/variant_calling_freebayes.nf.test.snap  |  24 ++--
 tests/variant_calling_msisensor2.nf.test.snap |   6 +-
 tests/variant_calling_mutect2.nf.test.snap    |   2 +-
 ...iant_calling_sentieon_tnscope.nf.test.snap |   8 +-
 workflows/sarek.nf                            |   3 -
 86 files changed, 1245 insertions(+), 998 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index f6ccbf673a..12fcf16783 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,6 +19,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2235](https://github.com/nf-core/sarek/pull/2235) - Nextflow strict-syntax / 26.x readiness for local code: explicit, named closure parameters (replacing implicit/generic `it`), `_`-prefixed unused parameters, and removal of unused `take:` inputs. Previously-dropped Manta candidate VCFs and Sentieon gVCF indices are now emitted.
 - [#2235](https://github.com/nf-core/sarek/pull/2235) - germline CNVKIT reuses the shared `CRAM_TO_BAM` conversion instead of re-converting CRAM internally, avoiding a duplicate conversion. Side effect: with `--step variant_calling` (user-supplied CRAM/BAM), CNVKit output files are named after the input file rather than the sample; runs from FASTQ are unaffected.
 - [#2238](https://github.com/nf-core/sarek/pull/2238) - Migrate `gatk4`/`gatk4spark` modules to the versions topic channel (bumps gatk4spark 4.6.1.0 → 4.6.2.0)
+- [#2239](https://github.com/nf-core/sarek/pull/2239) - Migrate alignment/UMI/utility modules (`bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`, `gunzip`, `untar`, `unzip`, `spring`) to the versions topic channel (fastp 0.24.0 → 1.1.0)
 
 ### Fixed
 
@@ -37,6 +38,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | ensembl-vep   | 115.2       | 116.0       |
 | gatk4         | 4.6.1.0     | 4.6.2.0     |
 | gatk4-spark   | 4.6.1.0     | 4.6.2.0     |
+| bwa           | 0.7.18      | 0.7.19      |
+| bwa-mem2      | 2.2.1       | 2.3         |
+| fastp         | 0.24.0      | 1.1.0       |
+| fgbio         | 2.4.0       | 3.1.2       |
+| gawk          | 5.3.0       | 5.3.1       |
+| pigz          | 2.3.4       | 2.8         |
 
 ### Dependencies - plugins
 
diff --git a/docs/output.md b/docs/output.md
index 613966cb85..044090836c 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -135,7 +135,7 @@ The resulting files are intermediate and by default not kept in the final files
 
 **Output directory: `{outdir}/preprocessing/fastp/<sample>`**
 
-- `<sample>_<lane>_{1,2}.fastp.fastq.gz>`
+- `<sample>_<lane>_{R1,R2}.fastp.fastq.gz>`
   - Bgzipped FastQ file
 
 </details>
@@ -151,7 +151,7 @@ These files are intermediate and by default not placed in the output-folder kept
 
 **Output directory: `{outdir}/preprocessing/fastp/<sample>/`**
 
-- `<sample_lane_{1,2}.fastp.fastq.gz>`
+- `<sample>_<lane>_{R1,R2}.fastp.fastq.gz>`
   - Bgzipped FastQ file
 
 </details>
diff --git a/modules.json b/modules.json
index 6ffabb8f5a..d7a3851c68 100644
--- a/modules.json
+++ b/modules.json
@@ -62,32 +62,32 @@
                     },
                     "bwa/index": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "bwa/mem": {
                         "branch": "master",
-                        "git_sha": "a29f18660f5e3748d44d6f716241e70c942c065d",
+                        "git_sha": "2fb127c8fd13de0adaa676df7169131e45c0b114",
                         "installed_by": ["modules"]
                     },
                     "bwamem2/index": {
                         "branch": "master",
-                        "git_sha": "b2902040b9cb9b7b32b62400f1c024a709bd4812",
+                        "git_sha": "62ce917fd775aaef732eb82019de069c765adfd3",
                         "installed_by": ["modules"]
                     },
                     "bwamem2/mem": {
                         "branch": "master",
-                        "git_sha": "a29f18660f5e3748d44d6f716241e70c942c065d",
+                        "git_sha": "62ce917fd775aaef732eb82019de069c765adfd3",
                         "installed_by": ["modules"]
                     },
                     "cat/cat": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "eac429d4c67ac5114ce7d4287d460d8f05fd9fab",
                         "installed_by": ["modules"]
                     },
                     "cat/fastq": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/antitarget": {
@@ -153,12 +153,12 @@
                     },
                     "dragmap/align": {
                         "branch": "master",
-                        "git_sha": "8b06d86f6a82b6203f239ad409f606fdf71ec697",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "dragmap/hashtable": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "ensemblvep/download": {
@@ -173,7 +173,7 @@
                     },
                     "fastp": {
                         "branch": "master",
-                        "git_sha": "d082103d7976a2804f21225446cc110cbd822f4c",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "fastqc": {
@@ -183,22 +183,22 @@
                     },
                     "fgbio/callmolecularconsensusreads": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "fgbio/copyumifromreadname": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "fgbio/fastqtobam": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "fgbio/groupreadsbyumi": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "freebayes": {
@@ -333,7 +333,7 @@
                     },
                     "gawk": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "goleft/indexcov": {
@@ -343,7 +343,7 @@
                     },
                     "gunzip": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "0902eac3012baaf4f9ab6513c8c55acc9353c96c",
                         "installed_by": ["modules"]
                     },
                     "lofreq/callparallel": {
@@ -528,7 +528,7 @@
                     },
                     "spring/decompress": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "strelka/germline": {
@@ -563,12 +563,12 @@
                     },
                     "untar": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "unzip": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "1c43a7e22f04cf19c9f9d3de7e7e5a3addd9382a",
                         "installed_by": ["modules"]
                     },
                     "varlociraptor/callvariants": {
diff --git a/modules/nf-core/bwa/index/environment.yml b/modules/nf-core/bwa/index/environment.yml
index ed5448a197..54e679492f 100644
--- a/modules/nf-core/bwa/index/environment.yml
+++ b/modules/nf-core/bwa/index/environment.yml
@@ -6,8 +6,8 @@ channels:
 
 dependencies:
   # renovate: datasource=conda depName=bioconda/bwa
-  - bioconda::bwa=0.7.18
+  - bioconda::bwa=0.7.19
   # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.21
+  - bioconda::htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/samtools
-  - bioconda::samtools=1.21
+  - bioconda::samtools=1.22.1
diff --git a/modules/nf-core/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf
index 72e078aac2..a1c98acee6 100644
--- a/modules/nf-core/bwa/index/main.nf
+++ b/modules/nf-core/bwa/index/main.nf
@@ -2,19 +2,19 @@ process BWA_INDEX {
     tag "$fasta"
     // NOTE requires 5.37N memory where N is the size of the database
     // source: https://bio-bwa.sourceforge.net/bwa.shtml#8
-    memory { 6.B * fasta.size() }
+    memory { 7.B * fasta.size() }
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/bf/bf7890f8d4e38a7586581cb7fa13401b7af1582f21d94eef969df4cea852b6da/data' :
-        'community.wave.seqera.io/library/bwa_htslib_samtools:56c9f8d5201889a4' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d7/d7e24dc1e4d93ca4d3a76a78d4c834a7be3985b0e1e56fddd61662e047863a8a/data' :
+        'community.wave.seqera.io/library/bwa_htslib_samtools:83b50ff84ead50d0' }"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
-    tuple val(meta), path("bwa")  , emit: index
-    path "versions.yml"             , emit: versions
+    tuple val(meta), path("bwa"), emit: index
+    tuple val("${task.process}"), val('bwa'), eval('bwa 2>&1 | sed -n "s/^Version: //p"'), topic: versions, emit: versions_bwa
 
     when:
     task.ext.when == null || task.ext.when
@@ -29,27 +29,16 @@ process BWA_INDEX {
         $args \\
         -p bwa/${prefix} \\
         $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//')
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${fasta.baseName}"
     """
     mkdir bwa
-
     touch bwa/${prefix}.amb
     touch bwa/${prefix}.ann
     touch bwa/${prefix}.bwt
     touch bwa/${prefix}.pac
     touch bwa/${prefix}.sa
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bwa/index/meta.yml b/modules/nf-core/bwa/index/meta.yml
index 1781586fa1..f5bf7f52a9 100644
--- a/modules/nf-core/bwa/index/meta.yml
+++ b/modules/nf-core/bwa/index/meta.yml
@@ -42,17 +42,30 @@ output:
           pattern: "*.{amb,ann,bwt,pac,sa}"
           ontologies:
             - edam: "http://edamontology.org/data_3210" # Genome index
+  versions_bwa:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bwa:
+          type: string
+          description: The tool name
+      - 'bwa 2>&1 | sed -n "s/^Version: //p"':
+          type: string
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - bwa:
+          type: string
+          description: The tool name
+      - 'bwa 2>&1 | sed -n "s/^Version: //p"':
+          type: string
+          description: The command used to generate the version of the tool
 authors:
   - "@drpatelh"
   - "@maxulysse"
 maintainers:
-  - "@drpatelh"
   - "@maxulysse"
   - "@gallvp"
diff --git a/modules/nf-core/bwa/mem/environment.yml b/modules/nf-core/bwa/mem/environment.yml
index ed5448a197..54e679492f 100644
--- a/modules/nf-core/bwa/mem/environment.yml
+++ b/modules/nf-core/bwa/mem/environment.yml
@@ -6,8 +6,8 @@ channels:
 
 dependencies:
   # renovate: datasource=conda depName=bioconda/bwa
-  - bioconda::bwa=0.7.18
+  - bioconda::bwa=0.7.19
   # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.21
+  - bioconda::htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/samtools
-  - bioconda::samtools=1.21
+  - bioconda::samtools=1.22.1
diff --git a/modules/nf-core/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf
index 3c54417824..bde6a9a334 100644
--- a/modules/nf-core/bwa/mem/main.nf
+++ b/modules/nf-core/bwa/mem/main.nf
@@ -3,9 +3,9 @@ process BWA_MEM {
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/bf/bf7890f8d4e38a7586581cb7fa13401b7af1582f21d94eef969df4cea852b6da/data' :
-        'community.wave.seqera.io/library/bwa_htslib_samtools:56c9f8d5201889a4' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/d7/d7e24dc1e4d93ca4d3a76a78d4c834a7be3985b0e1e56fddd61662e047863a8a/data' :
+        'community.wave.seqera.io/library/bwa_htslib_samtools:83b50ff84ead50d0' }"
 
     input:
     tuple val(meta) , path(reads)
@@ -16,9 +16,11 @@ process BWA_MEM {
     output:
     tuple val(meta), path("*.bam")  , emit: bam,    optional: true
     tuple val(meta), path("*.cram") , emit: cram,   optional: true
+    tuple val(meta), path("*.sam")  , emit: sam,    optional: true
     tuple val(meta), path("*.csi")  , emit: csi,    optional: true
     tuple val(meta), path("*.crai") , emit: crai,   optional: true
-    path  "versions.yml"            , emit: versions
+    tuple val("${task.process}"), val('bwa'), eval('bwa 2>&1 | sed -n "s/^Version: //p"'), topic: versions, emit: versions_bwa
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
@@ -35,6 +37,15 @@ process BWA_MEM {
                     "bam"
     def reference = fasta && extension=="cram"  ? "--reference ${fasta}" : ""
     if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output"
+    //
+    // For SAM output we can skip samtools view
+    //
+    def pipe_command = ""
+    if (extension == "sam") {
+        pipe_command = "> ${prefix}.${extension}"
+    } else {
+        pipe_command = "| samtools $samtools_command $args2 ${reference} --threads $task.cpus -o ${prefix}.${extension} -"
+    }
     """
     INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'`
 
@@ -43,13 +54,7 @@ process BWA_MEM {
         -t $task.cpus \\
         \$INDEX \\
         $reads \\
-        | samtools $samtools_command $args2 ${reference} --threads $task.cpus -o ${prefix}.${extension} -
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        $pipe_command
     """
 
     stub:
@@ -64,11 +69,5 @@ process BWA_MEM {
     touch ${prefix}.${extension}
     touch ${prefix}.csi
     touch ${prefix}.crai
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml
index b6f696c03b..1c4ee8833e 100644
--- a/modules/nf-core/bwa/mem/meta.yml
+++ b/modules/nf-core/bwa/mem/meta.yml
@@ -16,7 +16,8 @@ tools:
       homepage: http://bio-bwa.sourceforge.net/
       documentation: https://bio-bwa.sourceforge.net/bwa.shtml
       arxiv: arXiv:1303.3997
-      licence: ["GPL-3.0-or-later"]
+      licence:
+        - "GPL-3.0-or-later"
       identifier: "biotools:bwa"
 input:
   - - meta:
@@ -30,8 +31,8 @@ input:
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
         ontologies:
-          - edam: "http://edamontology.org/data_2044" # Sequence
-          - edam: "http://edamontology.org/format_1930" # FASTQ
+          - edam: "http://edamontology.org/data_2044"
+          - edam: "http://edamontology.org/format_1930"
   - - meta2:
         type: map
         description: |
@@ -42,7 +43,7 @@ input:
         description: BWA genome index files
         pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}"
         ontologies:
-          - edam: "http://edamontology.org/data_3210" # Genome index
+          - edam: "http://edamontology.org/data_3210"
   - - meta3:
         type: map
         description: |
@@ -53,54 +54,101 @@ input:
         description: Reference genome in FASTA format
         pattern: "*.{fasta,fa}"
         ontologies:
-          - edam: "http://edamontology.org/data_2044" # Sequence
-          - edam: "http://edamontology.org/format_1929" # FASTA
-  - - sort_bam:
-        type: boolean
-        description: use samtools sort (true) or samtools view (false)
-        pattern: "true or false"
+          - edam: "http://edamontology.org/data_2044"
+          - edam: "http://edamontology.org/format_1929"
+  - sort_bam:
+      type: boolean
+      description: use samtools sort (true) or samtools view (false)
+      pattern: "true or false"
 output:
-  - bam:
-      - meta:
-          type: file
-          description: Output BAM file containing read alignments
+  bam:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - "*.bam":
           type: file
           description: Output BAM file containing read alignments
           pattern: "*.{bam}"
           ontologies:
-            - edam: "http://edamontology.org/format_2572" # BAM
-  - cram:
-      - meta:
-          type: file
-          description: Output CRAM file containing read alignments
+            - edam: "http://edamontology.org/format_2572"
+  cram:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - "*.cram":
           type: file
           description: Output CRAM file containing read alignments
           pattern: "*.{cram}"
           ontologies:
-            - edam: "http://edamontology.org/format_3462" # CRAM
-  - csi:
-      - meta:
+            - edam: "http://edamontology.org/format_3462"
+  sam:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
+      - "*.sam":
           type: file
-          description: Optional index file for BAM file
+          description: Output SAM file containing read alignments
+          pattern: "*.{sam}"
+          ontologies:
+            - edam: "http://edamontology.org/format_2573"
+  csi:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - "*.csi":
           type: file
           description: Optional index file for BAM file
           pattern: "*.{csi}"
-  - crai:
-      - meta:
-          type: file
-          description: Optional index file for CRAM file
+          ontologies: []
+  crai:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - "*.crai":
           type: file
           description: Optional index file for CRAM file
           pattern: "*.{crai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_bwa:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwa:
+          type: string
+          description: The name of the tool
+      - 'bwa 2>&1 | sed -n "s/^Version: //p"':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwa:
+          type: string
+          description: The name of the tool
+      - 'bwa 2>&1 | sed -n "s/^Version: //p"':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@drpatelh"
   - "@jeremy1805"
diff --git a/modules/nf-core/bwamem2/index/environment.yml b/modules/nf-core/bwamem2/index/environment.yml
index c069e281ac..f3637444a6 100644
--- a/modules/nf-core/bwamem2/index/environment.yml
+++ b/modules/nf-core/bwamem2/index/environment.yml
@@ -6,8 +6,8 @@ channels:
 
 dependencies:
   # renovate: datasource=conda depName=bioconda/bwa-mem2
-  - bwa-mem2=2.2.1
+  - bwa-mem2=2.3
   # renovate: datasource=conda depName=bioconda/htslib
-  - htslib=1.21
+  - htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/samtools
-  - samtools=1.21
+  - samtools=1.22.1
diff --git a/modules/nf-core/bwamem2/index/main.nf b/modules/nf-core/bwamem2/index/main.nf
index 529c66e8fd..4d1461e637 100644
--- a/modules/nf-core/bwamem2/index/main.nf
+++ b/modules/nf-core/bwamem2/index/main.nf
@@ -2,19 +2,19 @@ process BWAMEM2_INDEX {
     tag "$fasta"
     // NOTE Requires 28N GB memory where N is the size of the reference sequence, floor of 280M
     // source: https://github.com/bwa-mem2/bwa-mem2/issues/9
-    memory { (280.MB * Math.ceil(fasta.size() / 10000000)) * task.attempt }
+    memory { 280.MB * Math.ceil(fasta.size() / 10000000) * task.attempt }
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9a/9ac054213e67b3c9308e409b459080bbe438f8fd6c646c351bc42887f35a42e7/data' :
-        'community.wave.seqera.io/library/bwa-mem2_htslib_samtools:e1f420694f8e42bd' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e0/e05ce34b46ad42810eb29f74e4e304c0cb592b2ca15572929ed8bbaee58faf01/data' :
+        'community.wave.seqera.io/library/bwa-mem2_htslib_samtools:db98f81f55b64113' }"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
     tuple val(meta), path("bwamem2"), emit: index
-    path "versions.yml"             , emit: versions
+    tuple val("${task.process}"), val('bwamem2'), eval('bwa-mem2 version | grep -o -E "[0-9]+(\\.[0-9]+)+"'), emit: versions_bwamem2, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -29,11 +29,6 @@ process BWAMEM2_INDEX {
         $args \\
         -p bwamem2/${prefix} \\
         $fasta
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwamem2: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //')
-    END_VERSIONS
     """
 
     stub:
@@ -46,10 +41,5 @@ process BWAMEM2_INDEX {
     touch bwamem2/${prefix}.pac
     touch bwamem2/${prefix}.amb
     touch bwamem2/${prefix}.bwt.2bit.64
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwamem2: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bwamem2/index/meta.yml b/modules/nf-core/bwamem2/index/meta.yml
index b2aa45fb65..336ab20243 100644
--- a/modules/nf-core/bwamem2/index/meta.yml
+++ b/modules/nf-core/bwamem2/index/meta.yml
@@ -12,7 +12,14 @@ tools:
         a large reference genome, such as the human genome.
       homepage: https://github.com/bwa-mem2/bwa-mem2
       documentation: https://github.com/bwa-mem2/bwa-mem2#usage
-      licence: ["MIT"]
+      doi: "10.1109/IPDPS.2019.00041"
+      publication:
+        author: "Vasimuddin M., Misra S., Li H. & Aluru S."
+        year: 2018
+        source: "IEEE International Parallel and Distributed Processing Symposium (IPDPS)"
+        title: "Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems"
+      licence:
+        - "MIT"
       identifier: "biotools:bwa-mem2"
 input:
   - - meta:
@@ -24,8 +31,8 @@ input:
         type: file
         description: Input genome fasta file
         ontologies:
-          - edam: "http://edamontology.org/data_2044" # Sequence
-          - edam: "http://edamontology.org/format_1929" # FASTA
+          - edam: "http://edamontology.org/data_2044"
+          - edam: "http://edamontology.org/format_1929"
 output:
   index:
     - - meta:
@@ -34,18 +41,38 @@ output:
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - bwamem2:
-          type: file
+          type: string
           description: BWA genome index files
           pattern: "*.{0123,amb,ann,bwt.2bit.64,pac}"
           ontologies:
-            - edam: "http://edamontology.org/data_3210" # Genome index
+            - edam: "http://edamontology.org/data_3210"
+  versions_bwamem2:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwamem2:
+          type: string
+          description: BWA genome index files
+          pattern: "*.{0123,amb,ann,bwt.2bit.64,pac}"
+          ontologies:
+            - edam: "http://edamontology.org/data_3210"
+      - bwa-mem2 version | grep -o -E "[0-9]+(\.[0-9]+)+":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwamem2:
+          type: string
+          description: BWA genome index files
+          pattern: "*.{0123,amb,ann,bwt.2bit.64,pac}"
+          ontologies:
+            - edam: "http://edamontology.org/data_3210"
+      - bwa-mem2 version | grep -o -E "[0-9]+(\.[0-9]+)+":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@maxulysse"
 maintainers:
diff --git a/modules/nf-core/bwamem2/mem/environment.yml b/modules/nf-core/bwamem2/mem/environment.yml
index c069e281ac..f3637444a6 100644
--- a/modules/nf-core/bwamem2/mem/environment.yml
+++ b/modules/nf-core/bwamem2/mem/environment.yml
@@ -6,8 +6,8 @@ channels:
 
 dependencies:
   # renovate: datasource=conda depName=bioconda/bwa-mem2
-  - bwa-mem2=2.2.1
+  - bwa-mem2=2.3
   # renovate: datasource=conda depName=bioconda/htslib
-  - htslib=1.21
+  - htslib=1.22.1
   # renovate: datasource=conda depName=bioconda/samtools
-  - samtools=1.21
+  - samtools=1.22.1
diff --git a/modules/nf-core/bwamem2/mem/main.nf b/modules/nf-core/bwamem2/mem/main.nf
index eab662a87a..9d827dc84e 100644
--- a/modules/nf-core/bwamem2/mem/main.nf
+++ b/modules/nf-core/bwamem2/mem/main.nf
@@ -1,25 +1,26 @@
 process BWAMEM2_MEM {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/9a/9ac054213e67b3c9308e409b459080bbe438f8fd6c646c351bc42887f35a42e7/data' :
-        'community.wave.seqera.io/library/bwa-mem2_htslib_samtools:e1f420694f8e42bd' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e0/e05ce34b46ad42810eb29f74e4e304c0cb592b2ca15572929ed8bbaee58faf01/data'
+        : 'community.wave.seqera.io/library/bwa-mem2_htslib_samtools:db98f81f55b64113'}"
 
     input:
     tuple val(meta), path(reads)
     tuple val(meta2), path(index)
     tuple val(meta3), path(fasta)
-    val   sort_bam
+    val sort_bam
 
     output:
-    tuple val(meta), path("*.sam")  , emit: sam , optional:true
-    tuple val(meta), path("*.bam")  , emit: bam , optional:true
-    tuple val(meta), path("*.cram") , emit: cram, optional:true
-    tuple val(meta), path("*.crai") , emit: crai, optional:true
-    tuple val(meta), path("*.csi")  , emit: csi , optional:true
-    path  "versions.yml"            , emit: versions
+    tuple val(meta), path("*.sam"), emit: sam, optional: true
+    tuple val(meta), path("*.bam"), emit: bam, optional: true
+    tuple val(meta), path("*.cram"), emit: cram, optional: true
+    tuple val(meta), path("*.crai"), emit: crai, optional: true
+    tuple val(meta), path("*.csi"), emit: csi, optional: true
+    tuple val("${task.process}"), val('bwamem2'), eval('bwa-mem2 version | grep -o -E "[0-9]+(\\.[0-9]+)+"'), emit: versions_bwamem2, topic: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -31,53 +32,43 @@ process BWAMEM2_MEM {
     def samtools_command = sort_bam ? 'sort' : 'view'
 
     def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/
-    def extension_matcher =  (args2 =~ extension_pattern)
+    def extension_matcher = (args2 =~ extension_pattern)
     def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam"
-    def reference = fasta && extension=="cram"  ? "--reference ${fasta}" : ""
-    if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output"
-
+    def reference = fasta && extension == "cram" ? "--reference ${fasta}" : ""
+    if (!fasta && extension == "cram") {
+        error("Fasta reference is required for CRAM output")
+    }
     """
     INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'`
 
     bwa-mem2 \\
         mem \\
-        $args \\
-        -t $task.cpus \\
+        ${args} \\
+        -t ${task.cpus} \\
         \$INDEX \\
-        $reads \\
-        | samtools $samtools_command $args2 -@ $task.cpus ${reference} -o ${prefix}.${extension} -
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwamem2: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        ${reads} \\
+        | samtools ${samtools_command} ${args2} -@ ${task.cpus} ${reference} -o ${prefix}.${extension} -
     """
 
     stub:
-
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/
-    def extension_matcher =  (args2 =~ extension_pattern)
+    def extension_matcher = (args2 =~ extension_pattern)
     def extension = extension_matcher.getCount() > 0 ? extension_matcher[0][2].toLowerCase() : "bam"
-    if (!fasta && extension=="cram") error "Fasta reference is required for CRAM output"
+    if (!fasta && extension == "cram") {
+        error("Fasta reference is required for CRAM output")
+    }
 
     def create_index = ""
     if (extension == "cram") {
         create_index = "touch ${prefix}.crai"
-    } else if (extension == "bam") {
+    }
+    else if (extension == "bam") {
         create_index = "touch ${prefix}.csi"
     }
-
     """
     touch ${prefix}.${extension}
     ${create_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bwamem2: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //')
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/bwamem2/mem/meta.yml b/modules/nf-core/bwamem2/mem/meta.yml
index d17e0dbd3a..967c8de04c 100644
--- a/modules/nf-core/bwamem2/mem/meta.yml
+++ b/modules/nf-core/bwamem2/mem/meta.yml
@@ -9,14 +9,20 @@ keywords:
   - bam
   - sam
 tools:
-  - bwa:
+  - bwamem2:
       description: |
         BWA-mem2 is a software package for mapping DNA sequences against
         a large reference genome, such as the human genome.
       homepage: https://github.com/bwa-mem2/bwa-mem2
-      documentation: http://www.htslib.org/doc/samtools.html
-      arxiv: arXiv:1303.3997
-      licence: ["MIT"]
+      documentation: https://github.com/bwa-mem2/bwa-mem2#usage
+      doi: "10.1109/IPDPS.2019.00041"
+      publication:
+        author: "Vasimuddin M., Misra S., Li H. & Aluru S."
+        year: 2018
+        source: "IEEE International Parallel and Distributed Processing Symposium (IPDPS)"
+        title: "Efficient Architecture-Aware Acceleration of BWA-MEM for Multicore Systems"
+      licence:
+        - "MIT"
       identifier: "biotools:bwa-mem2"
 input:
   - - meta:
@@ -30,8 +36,8 @@ input:
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
         ontologies:
-          - edam: "http://edamontology.org/data_2044" # Sequence
-          - edam: "http://edamontology.org/format_1930" # FASTQ
+          - edam: "http://edamontology.org/data_2044"
+          - edam: "http://edamontology.org/format_1930"
   - - meta2:
         type: map
         description: |
@@ -42,7 +48,7 @@ input:
         description: BWA genome index files
         pattern: "Directory containing BWA index *.{0132,amb,ann,bwt.2bit.64,pac}"
         ontologies:
-          - edam: "http://edamontology.org/data_3210" # Genome index
+          - edam: "http://edamontology.org/data_3210"
   - - meta3:
         type: map
         description: |
@@ -53,15 +59,15 @@ input:
         description: Reference genome in FASTA format
         pattern: "*.{fa,fasta,fna}"
         ontologies:
-          - edam: "http://edamontology.org/data_2044" # Sequence
-          - edam: "http://edamontology.org/format_1929" # FASTA
-  - - sort_bam:
-        type: boolean
-        description: use samtools sort (true) or samtools view (false)
-        pattern: "true or false"
+          - edam: "http://edamontology.org/data_2044"
+          - edam: "http://edamontology.org/format_1929"
+  - sort_bam:
+      type: boolean
+      description: use samtools sort (true) or samtools view (false)
+      pattern: "true or false"
 output:
-  - sam:
-      - meta:
+  sam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,9 +77,9 @@ output:
           description: Output SAM file containing read alignments
           pattern: "*.{sam}"
           ontologies:
-            - edam: "http://edamontology.org/format_2573" # SAM
-  - bam:
-      - meta:
+            - edam: "http://edamontology.org/format_2573"
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -83,9 +89,9 @@ output:
           description: Output BAM file containing read alignments
           pattern: "*.{bam}"
           ontologies:
-            - edam: "http://edamontology.org/format_2572" # BAM
-  - cram:
-      - meta:
+            - edam: "http://edamontology.org/format_2572"
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -95,9 +101,9 @@ output:
           description: Output CRAM file containing read alignments
           pattern: "*.{cram}"
           ontologies:
-            - edam: "http://edamontology.org/format_3462" # CRAM
-  - crai:
-      - meta:
+            - edam: "http://edamontology.org/format_3462"
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -106,8 +112,9 @@ output:
           type: file
           description: Index file for CRAM file
           pattern: "*.{crai}"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -116,11 +123,47 @@ output:
           type: file
           description: Index file for BAM file
           pattern: "*.{csi}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_bwamem2:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwamem2:
+          type: string
+          description: The name of the tool
+      - bwa-mem2 version | grep -o -E "[0-9]+(\.[0-9]+)+":
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bwamem2:
+          type: string
+          description: The name of the tool
+      - bwa-mem2 version | grep -o -E "[0-9]+(\.[0-9]+)+":
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@maxulysse"
   - "@matthdsm"
diff --git a/modules/nf-core/cat/cat/environment.yml b/modules/nf-core/cat/cat/environment.yml
index 50c2059afb..985117698a 100644
--- a/modules/nf-core/cat/cat/environment.yml
+++ b/modules/nf-core/cat/cat/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::pigz=2.3.4
+  - conda-forge::pigz=2.8
diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf
index 2862c64cd9..6f066e5f78 100644
--- a/modules/nf-core/cat/cat/main.nf
+++ b/modules/nf-core/cat/cat/main.nf
@@ -3,72 +3,48 @@ process CAT_CAT {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/pigz:2.3.4' :
-        'biocontainers/pigz:2.3.4' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/pigz:2.8' :
+        'quay.io/biocontainers/pigz:2.8' }"
 
     input:
     tuple val(meta), path(files_in)
 
     output:
     tuple val(meta), path("${prefix}"), emit: file_out
-    path "versions.yml"               , emit: versions
+    tuple val("${task.process}"), val("pigz"), eval("pigz --version 2>&1 | sed 's/pigz //g'"),  topic: versions, emit: versions_cat
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def file_list = files_in.collect { it.toString() }
-
-    // choose appropriate concatenation tool depending on input and output format
-
-    // | input     | output     | command1 | command2 |
-    // |-----------|------------|----------|----------|
-    // | gzipped   | gzipped    | cat      |          |
-    // | ungzipped | ungzipped  | cat      |          |
-    // | gzipped   | ungzipped  | zcat     |          |
-    // | ungzipped | gzipped    | cat      | pigz     |
-
-    // Use input file ending as default
-    prefix   = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}"
-    out_zip  = prefix.endsWith('.gz')
-    in_zip   = file_list[0].endsWith('.gz')
-    command1 = (in_zip && !out_zip) ? 'zcat' : 'cat'
-    command2 = (!in_zip && out_zip) ? "| pigz -c -p $task.cpus $args2" : ''
-    if(file_list.contains(prefix.trim())) {
-        error "The name of the input file can't be the same as for the output prefix in the " +
-        "module CAT_CAT (currently `$prefix`). Please choose a different one."
-    }
+    def deprecation_message = """
+    WARNING: This module has been deprecated. Please use nf-core/modules/find/concatenate
+
+    Reason:
+    This module passes all input files as shell arguments, which can exceed the UNIX ARG_MAX
+    limit when concatenating large numbers of files. The find/concatenate module resolves this
+    by staging files into a directory and enumerating them with `find`, and also enforces
+    consistent input compression (all gzipped or all uncompressed). Also enables faster, parallel
+    decompression with pigz.
     """
-    $command1 \\
-        $args \\
-        ${file_list.join(' ')} \\
-        $command2 \\
-        > ${prefix}
+    assert false: deprecation_message
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
-    """
+    def file_list = files_in.collect { file -> file.toString() }
+    prefix   = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}"
 
     stub:
-    def file_list   = files_in.collect { it.toString() }
-    prefix          = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}"
-    if(file_list.contains(prefix.trim())) {
-        error "The name of the input file can't be the same as for the output prefix in the " +
-        "module CAT_CAT (currently `$prefix`). Please choose a different one."
-    }
-    """
-    touch $prefix
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
+    def deprecation_message = """
+    WARNING: This module has been deprecated. Please use nf-core/modules/find/concatenate
+
+    Reason:
+    This module passes all input files as shell arguments, which can exceed the UNIX ARG_MAX
+    limit when concatenating large numbers of files. The find/concatenate module resolves this
+    by staging files into a directory and enumerating them with `find`, and also enforces
+    consistent input compression (all gzipped or all uncompressed). Also enables faster, parallel
+    decompression with pigz.
     """
+    assert false: deprecation_message
 }
 
 // for .gz files also include the second to last extension if it is present. E.g., .fasta.gz
diff --git a/modules/nf-core/cat/cat/meta.yml b/modules/nf-core/cat/cat/meta.yml
index 81778a0671..0163c33a58 100644
--- a/modules/nf-core/cat/cat/meta.yml
+++ b/modules/nf-core/cat/cat/meta.yml
@@ -1,5 +1,6 @@
 name: cat_cat
 description: A module for concatenation of gzipped or uncompressed files
+deprecated: true
 keywords:
   - concatenate
   - gzip
@@ -20,21 +21,41 @@ input:
         type: file
         description: List of compressed / uncompressed files
         pattern: "*"
+        ontologies: []
 output:
-  - file_out:
-      - meta:
-          type: file
-          description: Concatenated file. Will be gzipped if file_out ends with ".gz"
-          pattern: "${file_out}"
+  file_out:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information
       - ${prefix}:
           type: file
-          description: Concatenated file. Will be gzipped if file_out ends with ".gz"
+          description: Concatenated file. Will be gzipped if file_out ends with
+            ".gz"
           pattern: "${file_out}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_cat:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - pigz:
+          type: string
+          description: The name of the tool
+      - "pigz --version 2>&1 | sed 's/pigz //g'":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - pigz:
+          type: string
+          description: The name of the tool
+      - "pigz --version 2>&1 | sed 's/pigz //g'":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@erikrikarddaniel"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf
index acfb6d0e62..8d12a78cd3 100644
--- a/modules/nf-core/cat/fastq/main.nf
+++ b/modules/nf-core/cat/fastq/main.nf
@@ -3,7 +3,7 @@ process CAT_FASTQ {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/52/52ccce28d2ab928ab862e25aae26314d69c8e38bd41ca9431c67ef05221348aa/data'
         : 'community.wave.seqera.io/library/coreutils_grep_gzip_lbzip2_pruned:838ba80435a629f8'}"
 
@@ -12,23 +12,19 @@ process CAT_FASTQ {
 
     output:
     tuple val(meta), path("*.merged.fastq.gz"), emit: reads
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val("cat"), eval("cat --version 2>&1 | head -n 1 | sed 's/^.*coreutils) //; s/ .*\$//'"), emit: versions_cat, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()]
+    def readList = reads instanceof List ? reads.collect { item -> item.toString() } : [reads.toString()]
+    def compress = readList[0]?.endsWith('.gz') ? '' : '| gzip'
     if (meta.single_end) {
         if (readList.size >= 1) {
             """
-            cat ${readList.join(' ')} > ${prefix}.merged.fastq.gz
-
-            cat <<-END_VERSIONS > versions.yml
-            "${task.process}":
-                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
-            END_VERSIONS
+            cat ${readList.join(' ')} ${compress} > ${prefix}.merged.fastq.gz
             """
         } else {
             error("Could not find any FASTQ files to concatenate in the process input")
@@ -40,13 +36,8 @@ process CAT_FASTQ {
             def read2 = []
             readList.eachWithIndex { v, ix -> (ix & 1 ? read2 : read1) << v }
             """
-            cat ${read1.join(' ')} > ${prefix}_1.merged.fastq.gz
-            cat ${read2.join(' ')} > ${prefix}_2.merged.fastq.gz
-
-            cat <<-END_VERSIONS > versions.yml
-            "${task.process}":
-                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
-            END_VERSIONS
+            cat ${read1.join(' ')} ${compress} > ${prefix}_1.merged.fastq.gz
+            cat ${read2.join(' ')} ${compress} > ${prefix}_2.merged.fastq.gz
             """
         } else {
             error("Could not find any FASTQ file pairs to concatenate in the process input")
@@ -55,16 +46,11 @@ process CAT_FASTQ {
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def readList = reads instanceof List ? reads.collect { it.toString() } : [reads.toString()]
+    def readList = reads instanceof List ? reads.collect { item -> item.toString() } : [reads.toString()]
     if (meta.single_end) {
         if (readList.size >= 1) {
             """
             echo '' | gzip > ${prefix}.merged.fastq.gz
-
-            cat <<-END_VERSIONS > versions.yml
-            "${task.process}":
-                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
-            END_VERSIONS
             """
         } else {
             error("Could not find any FASTQ files to concatenate in the process input")
@@ -75,11 +61,6 @@ process CAT_FASTQ {
             """
             echo '' | gzip > ${prefix}_1.merged.fastq.gz
             echo '' | gzip > ${prefix}_2.merged.fastq.gz
-
-            cat <<-END_VERSIONS > versions.yml
-            "${task.process}":
-                cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
-            END_VERSIONS
             """
         } else {
             error("Could not find any FASTQ file pairs to concatenate in the process input")
diff --git a/modules/nf-core/cat/fastq/meta.yml b/modules/nf-core/cat/fastq/meta.yml
index 91ff2fb5f6..6fefd6e0ec 100644
--- a/modules/nf-core/cat/fastq/meta.yml
+++ b/modules/nf-core/cat/fastq/meta.yml
@@ -1,9 +1,10 @@
 name: cat_fastq
-description: Concatenates fastq files
+description: Concatenates fastq files. Supports both compressed (.gz) and uncompressed inputs; uncompressed files are automatically gzip-compressed during concatenation.
 keywords:
   - cat
   - fastq
   - concatenate
+  - compress
 tools:
   - cat:
       description: |
@@ -21,9 +22,11 @@ input:
         type: file
         description: |
           List of input FastQ files to be concatenated.
+          Accepts both gzip-compressed (.fastq.gz) and uncompressed (.fastq) files.
+        ontologies: []
 output:
-  - reads:
-      - meta:
+  reads:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -32,11 +35,30 @@ output:
           type: file
           description: Merged fastq file
           pattern: "*.{merged.fastq.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_cat:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - cat:
+          type: string
+          description: The tool name
+      - cat --version 2>&1 | head -n 1 | sed 's/^.*coreutils) //; s/ .*\$//':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - cat:
+          type: string
+          description: The tool name
+      - cat --version 2>&1 | head -n 1 | sed 's/^.*coreutils) //; s/ .*\$//':
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/dragmap/align/main.nf b/modules/nf-core/dragmap/align/main.nf
index 3f6ea75366..045443e643 100644
--- a/modules/nf-core/dragmap/align/main.nf
+++ b/modules/nf-core/dragmap/align/main.nf
@@ -4,9 +4,9 @@ process DRAGMAP_ALIGN {
 
     conda "${moduleDir}/environment.yml"
     // WARN: Do not update this tool to 1.3.0 until https://github.com/Illumina/DRAGMAP/issues/47 is resolved
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/mulled-v2-580d344d9d4a496cd403932da8765f9e0187774d:df80ed8d23d0a2c43181a2b3dd1b39f2d00fab5c-0'
-        : 'biocontainers/mulled-v2-580d344d9d4a496cd403932da8765f9e0187774d:df80ed8d23d0a2c43181a2b3dd1b39f2d00fab5c-0'}"
+        : 'quay.io/biocontainers/mulled-v2-580d344d9d4a496cd403932da8765f9e0187774d:df80ed8d23d0a2c43181a2b3dd1b39f2d00fab5c-0'}"
 
     input:
     tuple val(meta), path(reads)
@@ -21,7 +21,9 @@ process DRAGMAP_ALIGN {
     tuple val(meta), path("*.crai"), emit: crai, optional: true
     tuple val(meta), path("*.csi"),  emit: csi,  optional: true
     tuple val(meta), path('*.log'),  emit: log
-    path "versions.yml",             emit: versions
+    tuple val("${task.process}"), val('dragmap'), eval("dragen-os --version 2>&1"), emit: versions_dragmap, topic: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), emit: versions_samtools, topic: versions
+    tuple val("${task.process}"), val('pigz'), eval("pigz --version 2>&1 | sed 's/pigz //'"), emit: versions_pigz, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -46,15 +48,8 @@ process DRAGMAP_ALIGN {
         ${args} \\
         --num-threads ${task.cpus} \\
         ${reads_command} \\
-        2> >(tee ${prefix}.dragmap.log >&2) \\
+        2>| >(tee ${prefix}.dragmap.log >&2) \\
         | samtools ${samtools_command} ${args2} --threads ${task.cpus} ${reference} -o ${prefix}.${extension} -
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        dragmap: \$(echo \$(dragen-os --version 2>&1))
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
     """
 
     stub:
@@ -79,12 +74,5 @@ process DRAGMAP_ALIGN {
     touch ${prefix}.${extension}
     ${create_index}
     touch ${prefix}.log
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        dragmap: \$(echo \$(dragen-os --version 2>&1))
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/dragmap/align/meta.yml b/modules/nf-core/dragmap/align/meta.yml
index 80f020f58f..bdb7f00a80 100644
--- a/modules/nf-core/dragmap/align/meta.yml
+++ b/modules/nf-core/dragmap/align/meta.yml
@@ -12,7 +12,8 @@ tools:
       homepage: https://github.com/Illumina/dragmap
       documentation: https://github.com/Illumina/dragmap
       tool_dev_url: https://github.com/Illumina/dragmap#basic-command-line-usage
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: ""
 input:
   - - meta:
@@ -25,6 +26,8 @@ input:
         description: |
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively.
+        ontologies:
+          - edam: http://edamontology.org/format_1930
   - - meta2:
         type: map
         description: |
@@ -34,6 +37,7 @@ input:
         type: file
         description: DRAGMAP hash table
         pattern: "Directory containing DRAGMAP hash table *.{cmp,.bin,.txt}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -43,12 +47,14 @@ input:
         type: file
         description: Genome fasta reference files
         pattern: "*.{fa,fasta,fna}"
-  - - sort_bam:
-        type: boolean
-        description: Sort the BAM file
+        ontologies:
+          - edam: http://edamontology.org/format_1929
+  - sort_bam:
+      type: boolean
+      description: Sort the BAM file
 output:
-  - sam:
-      - meta:
+  sam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -57,17 +63,22 @@ output:
           type: file
           description: Output SAM file containing read alignments
           pattern: "*.{sam}"
-  - bam:
-      - meta:
-          type: file
-          description: Output BAM file containing read alignments
-          pattern: "*.{bam}"
+          ontologies:
+            - edam: http://edamontology.org/format_2571
+  bam:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
       - "*.bam":
           type: file
           description: Output BAM file containing read alignments
           pattern: "*.{bam}"
-  - cram:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_2572
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -76,8 +87,10 @@ output:
           type: file
           description: Output CRAM file containing read alignments
           pattern: "*.{cram}"
-  - crai:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_2573
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -86,8 +99,9 @@ output:
           type: file
           description: Index file for CRAM file
           pattern: "*.{crai}"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -96,8 +110,9 @@ output:
           type: file
           description: Index file for CRAM file
           pattern: "*.{csi}"
-  - log:
-      - meta:
+          ontologies: []
+  log:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -106,11 +121,67 @@ output:
           type: file
           description: Log file
           pattern: "*.{log}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3888
+  versions_dragmap:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - dragmap:
+          type: string
+          description: The name of the tool
+      - dragen-os --version 2>&1:
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_pigz:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - pigz:
+          type: string
+          description: The name of the tool
+      - pigz --version 2>&1 | sed 's/pigz //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - dragmap:
+          type: string
+          description: The name of the tool
+      - dragen-os --version 2>&1:
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - pigz:
+          type: string
+          description: The name of the tool
+      - pigz --version 2>&1 | sed 's/pigz //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@edmundmiller"
 maintainers:
diff --git a/modules/nf-core/dragmap/hashtable/main.nf b/modules/nf-core/dragmap/hashtable/main.nf
index e86b110094..ff49b73e3d 100644
--- a/modules/nf-core/dragmap/hashtable/main.nf
+++ b/modules/nf-core/dragmap/hashtable/main.nf
@@ -4,16 +4,16 @@ process DRAGMAP_HASHTABLE {
 
     conda "${moduleDir}/environment.yml"
     // WARN: Do not update this tool to 1.3.0 until https://github.com/Illumina/DRAGMAP/issues/47 is resolved
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/dragmap:1.2.1--h72d16da_1'
-        : 'biocontainers/dragmap:1.2.1--h72d16da_1'}"
+        : 'quay.io/biocontainers/dragmap:1.2.1--h72d16da_1'}"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
     tuple val(meta), path("dragmap"), emit: hashmap
-    path "versions.yml",              emit: versions
+    tuple val("${task.process}"), val('dragmap'), eval("dragen-os --version 2>&1"), emit: versions_dragmap, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -28,20 +28,10 @@ process DRAGMAP_HASHTABLE {
         --output-directory dragmap \\
         ${args} \\
         --ht-num-threads ${task.cpus}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        dragmap: \$(echo \$(dragen-os --version 2>&1))
-    END_VERSIONS
     """
 
     stub:
     """
     mkdir dragmap
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        dragmap: \$(echo \$(dragen-os --version 2>&1))
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/dragmap/hashtable/meta.yml b/modules/nf-core/dragmap/hashtable/meta.yml
index c6367b4739..5d2e3929fa 100644
--- a/modules/nf-core/dragmap/hashtable/meta.yml
+++ b/modules/nf-core/dragmap/hashtable/meta.yml
@@ -11,7 +11,8 @@ tools:
       homepage: https://github.com/Illumina/dragmap
       documentation: https://github.com/Illumina/dragmap
       tool_dev_url: https://github.com/Illumina/dragmap#basic-command-line-usage
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: ""
 input:
   - - meta:
@@ -22,7 +23,8 @@ input:
     - fasta:
         type: file
         description: Input genome fasta file
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_1929
 output:
   hashmap:
     - - meta:
@@ -31,17 +33,35 @@ output:
             Groovy Map containing reference information
             e.g. [ id:'test', single_end:false ]
       - dragmap:
-          type: file
+          type: string
+          description: DRAGMAP hash table
+          pattern: "*.{cmp,.bin,.txt}"
+          ontologies: []
+  versions_dragmap:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - dragmap:
+          type: string
           description: DRAGMAP hash table
           pattern: "*.{cmp,.bin,.txt}"
           ontologies: []
+      - dragen-os --version 2>&1:
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - dragmap:
+          type: string
+          description: DRAGMAP hash table
+          pattern: "*.{cmp,.bin,.txt}"
+          ontologies: []
+      - dragen-os --version 2>&1:
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@edmundmiller"
 maintainers:
diff --git a/modules/nf-core/fastp/environment.yml b/modules/nf-core/fastp/environment.yml
index 90adcd2c52..b04ba3680e 100644
--- a/modules/nf-core/fastp/environment.yml
+++ b/modules/nf-core/fastp/environment.yml
@@ -4,4 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fastp=0.24.0
+  # renovate: datasource=conda depName=bioconda/fastp
+  - bioconda::fastp=1.1.0
diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf
index 1342741d53..7b58e32273 100644
--- a/modules/nf-core/fastp/main.nf
+++ b/modules/nf-core/fastp/main.nf
@@ -3,13 +3,12 @@ process FASTP {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/88/889a182b8066804f4799f3808a5813ad601381a8a0e3baa4ab8d73e739b97001/data' :
-        'community.wave.seqera.io/library/fastp:0.24.0--62c97b06e8447690' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/55/556474e164daf5a5e218cd5d497681dcba0645047cf24698f88e3e078eacbd09/data' :
+        'community.wave.seqera.io/library/fastp:1.1.0--08aa7c5662a30d57' }"
 
     input:
-    tuple val(meta), path(reads)
-    path  adapter_fasta
+    tuple val(meta), path(reads), path(adapter_fasta)
     val   discard_trimmed_pass
     val   save_trimmed_fail
     val   save_merged
@@ -21,7 +20,7 @@ process FASTP {
     tuple val(meta), path('*.log')            , emit: log
     tuple val(meta), path('*.fail.fastq.gz')  , optional:true, emit: reads_fail
     tuple val(meta), path('*.merged.fastq.gz'), optional:true, emit: reads_merged
-    path "versions.yml"                       , emit: versions
+    tuple val("${task.process}"), val('fastp'), eval('fastp --version 2>&1 | sed -e "s/fastp //g"'), emit: versions_fastp, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,9 +29,9 @@ process FASTP {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : ""
-    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
-    def out_fq1 = discard_trimmed_pass ?: ( meta.single_end ? "--out1 ${prefix}.fastp.fastq.gz" : "--out1 ${prefix}_1.fastp.fastq.gz" )
-    def out_fq2 = discard_trimmed_pass ?: "--out2 ${prefix}_2.fastp.fastq.gz"
+    def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_R1.fail.fastq.gz --unpaired2 ${prefix}_R2.fail.fastq.gz" : ''
+    def out_fq1 = discard_trimmed_pass ?: ( meta.single_end ? "--out1 ${prefix}.fastp.fastq.gz" : "--out1 ${prefix}_R1.fastp.fastq.gz" )
+    def out_fq2 = discard_trimmed_pass ?: "--out2 ${prefix}_R2.fastp.fastq.gz"
     // Added soft-links to original fastqs for consistent naming in MultiQC
     // Use single ended for interleaved. Add --interleaved_in in config.
     if ( task.ext.args?.contains('--interleaved_in') ) {
@@ -48,13 +47,8 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2) \\
+            2>| >(tee ${prefix}.fastp.log >&2) \\
         | gzip -c > ${prefix}.fastp.fastq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
-        END_VERSIONS
         """
     } else if (meta.single_end) {
         """
@@ -69,21 +63,16 @@ process FASTP {
             $adapter_list \\
             $fail_fastq \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2)
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
-        END_VERSIONS
+            2>| >(tee ${prefix}.fastp.log >&2)
         """
     } else {
         def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : ''
         """
-        [ ! -f  ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz
-        [ ! -f  ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz
+        [ ! -f  ${prefix}_R1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_R1.fastq.gz
+        [ ! -f  ${prefix}_R2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_R2.fastq.gz
         fastp \\
-            --in1 ${prefix}_1.fastq.gz \\
-            --in2 ${prefix}_2.fastq.gz \\
+            --in1 ${prefix}_R1.fastq.gz \\
+            --in2 ${prefix}_R2.fastq.gz \\
             $out_fq1 \\
             $out_fq2 \\
             --json ${prefix}.fastp.json \\
@@ -94,21 +83,16 @@ process FASTP {
             --thread $task.cpus \\
             --detect_adapter_for_pe \\
             $args \\
-            2> >(tee ${prefix}.fastp.log >&2)
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
-        END_VERSIONS
+            2>| >(tee ${prefix}.fastp.log >&2)
         """
     }
 
     stub:
     def prefix              = task.ext.prefix ?: "${meta.id}"
     def is_single_output    = task.ext.args?.contains('--interleaved_in') || meta.single_end
-    def touch_reads         = (discard_trimmed_pass) ? "" : (is_single_output) ? "echo '' | gzip > ${prefix}.fastp.fastq.gz" : "echo '' | gzip > ${prefix}_1.fastp.fastq.gz ; echo '' | gzip > ${prefix}_2.fastp.fastq.gz"
+    def touch_reads         = (discard_trimmed_pass) ? "" : (is_single_output) ? "echo '' | gzip > ${prefix}.fastp.fastq.gz" : "echo '' | gzip > ${prefix}_R1.fastp.fastq.gz ; echo '' | gzip > ${prefix}_R2.fastp.fastq.gz"
     def touch_merged        = (!is_single_output && save_merged) ? "echo '' | gzip >  ${prefix}.merged.fastq.gz" : ""
-    def touch_fail_fastq    = (!save_trimmed_fail) ? "" : meta.single_end ? "echo '' | gzip > ${prefix}.fail.fastq.gz" : "echo '' | gzip > ${prefix}.paired.fail.fastq.gz ; echo '' | gzip > ${prefix}_1.fail.fastq.gz ; echo '' | gzip > ${prefix}_2.fail.fastq.gz"
+    def touch_fail_fastq    = (!save_trimmed_fail) ? "" : meta.single_end ? "echo '' | gzip > ${prefix}.fail.fastq.gz" : "echo '' | gzip > ${prefix}.paired.fail.fastq.gz ; echo '' | gzip > ${prefix}_R1.fail.fastq.gz ; echo '' | gzip > ${prefix}_R2.fail.fastq.gz"
     """
     $touch_reads
     $touch_fail_fastq
@@ -116,10 +100,5 @@ process FASTP {
     touch "${prefix}.fastp.json"
     touch "${prefix}.fastp.html"
     touch "${prefix}.fastp.log"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml
index 9c4b245844..a67be395b8 100644
--- a/modules/nf-core/fastp/meta.yml
+++ b/modules/nf-core/fastp/meta.yml
@@ -24,25 +24,27 @@ input:
           List of input FastQ files of size 1 and 2 for single-end and paired-end data,
           respectively. If you wish to run interleaved paired-end data,  supply as single-end data
           but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module.
-  - - adapter_fasta:
+        ontologies: []
+    - adapter_fasta:
         type: file
         description: File in FASTA format containing possible adapters to remove.
         pattern: "*.{fasta,fna,fas,fa}"
-  - - discard_trimmed_pass:
-        type: boolean
-        description: |
-          Specify true to not write any reads that pass trimming thresholds.
-          This can be used to use fastp for the output report only.
-  - - save_trimmed_fail:
-        type: boolean
-        description: Specify true to save files that failed to pass trimming thresholds
-          ending in `*.fail.fastq.gz`
-  - - save_merged:
-        type: boolean
-        description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
+        ontologies: []
+  - discard_trimmed_pass:
+      type: boolean
+      description: |
+        Specify true to not write any reads that pass trimming thresholds.
+        This can be used to use fastp for the output report only.
+  - save_trimmed_fail:
+      type: boolean
+      description: Specify true to save files that failed to pass trimming thresholds
+        ending in `*.fail.fastq.gz`
+  - save_merged:
+      type: boolean
+      description: Specify true to save all merged reads to a file ending in `*.merged.fastq.gz`
 output:
-  - reads:
-      - meta:
+  reads:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,8 +53,11 @@ output:
           type: file
           description: The trimmed/modified/unmerged fastq reads
           pattern: "*fastp.fastq.gz"
-  - json:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_1930 # FASTQ
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  json:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -61,8 +66,10 @@ output:
           type: file
           description: Results in JSON format
           pattern: "*.json"
-  - html:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3464 # JSON
+  html:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,8 +78,9 @@ output:
           type: file
           description: Results in HTML format
           pattern: "*.html"
-  - log:
-      - meta:
+          ontologies: []
+  log:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,8 +89,9 @@ output:
           type: file
           description: fastq log file
           pattern: "*.log"
-  - reads_fail:
-      - meta:
+          ontologies: []
+  reads_fail:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,8 +100,11 @@ output:
           type: file
           description: Reads the failed the preprocessing
           pattern: "*fail.fastq.gz"
-  - reads_merged:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_1930 # FASTQ
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  reads_merged:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -101,14 +113,32 @@ output:
           type: file
           description: Reads that were successfully merged
           pattern: "*.{merged.fastq.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_fastp:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - fastp:
+          type: string
+          description: The name of the tool
+      - 'fastp --version 2>&1 | sed -e "s/fastp //g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - fastp:
+          type: string
+          description: The name of the tool
+      - 'fastp --version 2>&1 | sed -e "s/fastp //g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@drpatelh"
   - "@kevinmenden"
+  - "@eit-maxlcummins"
 maintainers:
   - "@drpatelh"
   - "@kevinmenden"
diff --git a/modules/nf-core/fgbio/callmolecularconsensusreads/environment.yml b/modules/nf-core/fgbio/callmolecularconsensusreads/environment.yml
index 4ebc0924d7..6af3be6aa4 100644
--- a/modules/nf-core/fgbio/callmolecularconsensusreads/environment.yml
+++ b/modules/nf-core/fgbio/callmolecularconsensusreads/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fgbio=2.4.0
+  - bioconda::fgbio=3.1.2
diff --git a/modules/nf-core/fgbio/callmolecularconsensusreads/main.nf b/modules/nf-core/fgbio/callmolecularconsensusreads/main.nf
index 7d2e660d02..bf07bcd9e2 100644
--- a/modules/nf-core/fgbio/callmolecularconsensusreads/main.nf
+++ b/modules/nf-core/fgbio/callmolecularconsensusreads/main.nf
@@ -1,11 +1,11 @@
 process FGBIO_CALLMOLECULARCONSENSUSREADS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/87/87626ef674e2f19366ae6214575a114fe80ce598e796894820550731706a84be/data' :
-        'community.wave.seqera.io/library/fgbio:2.4.0--913bad9d47ff8ddc' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4d/4d1150a2e123f49f8c268f0ab429847afae642376fa52af713b846b084df4a9f/data'
+        : 'community.wave.seqera.io/library/fgbio:3.1.2--6e9400d507a9dc55'}"
 
     input:
     tuple val(meta), path(grouped_bam)
@@ -14,7 +14,7 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS {
 
     output:
     tuple val(meta), path("*.bam"), emit: bam
-    path  "versions.yml"          , emit: versions
+    tuple val("${task.process}"), val('fgbio'), eval('fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\\[.*$//"'), topic: versions, emit: versions_fgbio
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,11 +24,14 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS {
     def prefix = task.ext.prefix ?: "${meta.id}_consensus_unmapped"
     def mem_gb = 8
     if (!task.memory) {
-        log.info '[fgbio CallMolecularConsensusReads] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.'
-    } else {
+        log.info('[fgbio CallMolecularConsensusReads] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.')
+    }
+    else {
         mem_gb = task.memory.giga
     }
-    if ("$grouped_bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${grouped_bam}" == "${prefix}.bam") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
     """
     fgbio \\
         -Xmx${mem_gb}g \\
@@ -36,29 +39,20 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS {
         --async-io=true \\
         --compression=1 \\
         CallMolecularConsensusReads \\
-        --input $grouped_bam \\
+        --input ${grouped_bam} \\
         --output ${prefix}.bam \\
         --min-reads ${min_reads} \\
         --min-input-base-quality ${min_baseq} \\
         --threads ${task.cpus} \\
-        $args;
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
+        ${args}
     """
 
     stub:
     prefix = task.ext.prefix ?: "${meta.id}_consensus_unmapped"
-    if ("$grouped_bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${grouped_bam}" == "${prefix}.bam") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
     """
     touch ${prefix}.bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
     """
-
 }
diff --git a/modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml b/modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml
index 846c297b19..6bc57de7d2 100644
--- a/modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml
+++ b/modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml
@@ -1,12 +1,14 @@
 name: fgbio_callmolecularconsensusreads
-description: Calls consensus sequences from reads with the same unique molecular tag.
+description: Calls consensus sequences from reads with the same unique molecular
+  tag.
 keywords:
   - UMIs
   - consensus sequence
   - bam
 tools:
   - fgbio:
-      description: Tools for working with genomic and high throughput sequencing data.
+      description: Tools for working with genomic and high throughput sequencing
+        data.
       homepage: https://github.com/fulcrumgenomics/fgbio
       documentation: http://fulcrumgenomics.github.io/fgbio/
       licence: ["MIT"]
@@ -22,15 +24,16 @@ input:
         description: |
           The input SAM or BAM file, grouped by UMIs
         pattern: "*.{bam,sam}"
-  - - min_reads:
-        type: integer
-        description: Minimum number of original reads to build each consensus read.
-  - - min_baseq:
-        type: integer
-        description: Ignore bases in raw reads that have Q below this value.
+        ontologies: []
+  - min_reads:
+      type: integer
+      description: Minimum number of original reads to build each consensus read.
+  - min_baseq:
+      type: integer
+      description: Ignore bases in raw reads that have Q below this value.
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -40,11 +43,29 @@ output:
           description: |
             Output SAM or BAM file to write consensus reads.
           pattern: "*.{bam,sam}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_fgbio:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@sruthipsuresh"
 maintainers:
diff --git a/modules/nf-core/fgbio/copyumifromreadname/environment.yml b/modules/nf-core/fgbio/copyumifromreadname/environment.yml
index 4ebc0924d7..6af3be6aa4 100644
--- a/modules/nf-core/fgbio/copyumifromreadname/environment.yml
+++ b/modules/nf-core/fgbio/copyumifromreadname/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fgbio=2.4.0
+  - bioconda::fgbio=3.1.2
diff --git a/modules/nf-core/fgbio/copyumifromreadname/main.nf b/modules/nf-core/fgbio/copyumifromreadname/main.nf
index b15c970af1..eafccacf51 100644
--- a/modules/nf-core/fgbio/copyumifromreadname/main.nf
+++ b/modules/nf-core/fgbio/copyumifromreadname/main.nf
@@ -1,11 +1,11 @@
 process FGBIO_COPYUMIFROMREADNAME {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/87/87626ef674e2f19366ae6214575a114fe80ce598e796894820550731706a84be/data' :
-        'community.wave.seqera.io/library/fgbio:2.4.0--913bad9d47ff8ddc' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4d/4d1150a2e123f49f8c268f0ab429847afae642376fa52af713b846b084df4a9f/data'
+        : 'community.wave.seqera.io/library/fgbio:3.1.2--6e9400d507a9dc55'}"
 
     input:
     tuple val(meta), path(bam), path(bai)
@@ -13,22 +13,23 @@ process FGBIO_COPYUMIFROMREADNAME {
     output:
     tuple val(meta), path("*.bam"), emit: bam
     tuple val(meta), path("*.bai"), emit: bai
-    path "versions.yml"           , emit: versions
+    tuple val("${task.process}"), val('fgbio'), eval('fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\\[.*$//"'), topic: versions, emit: versions_fgbio
 
     when:
     task.ext.when == null || task.ext.when
 
-
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}_umi_extracted"
     def mem_gb = 8
     if (!task.memory) {
-        log.info '[fgbio CopyUmiFromReadName] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.'
-    } else if (mem_gb > task.memory.giga) {
+        log.info('[fgbio CopyUmiFromReadName] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.')
+    }
+    else if (mem_gb > task.memory.giga) {
         if (task.memory.giga < 2) {
             mem_gb = 1
-        } else {
+        }
+        else {
             mem_gb = task.memory.giga - 1
         }
     }
@@ -41,24 +42,12 @@ process FGBIO_COPYUMIFROMREADNAME {
         ${args} \\
         --input ${bam} \\
         --output ${prefix}.bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
     """
 
-
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}_umi_extracted"
     """
-
     touch ${prefix}.bam
     touch ${prefix}.bai
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$(fgbio --version)
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fgbio/copyumifromreadname/meta.yml b/modules/nf-core/fgbio/copyumifromreadname/meta.yml
index 7e7b30f71d..27a9825e82 100644
--- a/modules/nf-core/fgbio/copyumifromreadname/meta.yml
+++ b/modules/nf-core/fgbio/copyumifromreadname/meta.yml
@@ -2,9 +2,10 @@
 name: "fgbio_copyumifromreadname"
 description: Copies the UMI at the end of a bam files read name to the RX tag.
 keywords:
-  - sort
-  - example
-  - genomics
+  - fgbio
+  - copy
+  - umi
+  - readname
 tools:
   - "fgbio":
       description: "A set of tools for working with genomic and high throughput sequencing
@@ -65,14 +66,28 @@ output:
           pattern: "*.{bai}"
           ontologies:
             - edam: "http://edamontology.org/format_3327" # BAI
-  versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
+  versions_fgbio:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
 
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@sppearce"
 maintainers:
diff --git a/modules/nf-core/fgbio/fastqtobam/environment.yml b/modules/nf-core/fgbio/fastqtobam/environment.yml
index 4ebc0924d7..6af3be6aa4 100644
--- a/modules/nf-core/fgbio/fastqtobam/environment.yml
+++ b/modules/nf-core/fgbio/fastqtobam/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fgbio=2.4.0
+  - bioconda::fgbio=3.1.2
diff --git a/modules/nf-core/fgbio/fastqtobam/main.nf b/modules/nf-core/fgbio/fastqtobam/main.nf
index b4223db0f8..bc7b7faad4 100644
--- a/modules/nf-core/fgbio/fastqtobam/main.nf
+++ b/modules/nf-core/fgbio/fastqtobam/main.nf
@@ -1,19 +1,18 @@
 process FGBIO_FASTQTOBAM {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/87/87626ef674e2f19366ae6214575a114fe80ce598e796894820550731706a84be/data' :
-        'community.wave.seqera.io/library/fgbio:2.4.0--913bad9d47ff8ddc' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4d/4d1150a2e123f49f8c268f0ab429847afae642376fa52af713b846b084df4a9f/data'
+        : 'community.wave.seqera.io/library/fgbio:3.1.2--6e9400d507a9dc55'}"
 
     input:
     tuple val(meta), path(reads)
 
     output:
-    tuple val(meta), path("*.bam") , emit: bam , optional: true
-    tuple val(meta), path("*.cram"), emit: cram, optional: true
-    path "versions.yml"            , emit: versions
+    tuple val(meta), path("*.{bam,cram}"), emit: bam
+    tuple val("${task.process}"), val('fgbio'), eval('fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\\[.*$//"'), topic: versions, emit: versions_fgbio
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,11 +26,13 @@ process FGBIO_FASTQTOBAM {
 
     def mem_gb = 8
     if (!task.memory) {
-        log.info '[fgbio FastqToBam] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.'
-    } else if (mem_gb > task.memory.giga) {
+        log.info('[fgbio FastqToBam] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.')
+    }
+    else if (mem_gb > task.memory.giga) {
         if (task.memory.giga < 2) {
             mem_gb = 1
-        } else {
+        }
+        else {
             mem_gb = task.memory.giga - 1
         }
     }
@@ -47,24 +48,12 @@ process FGBIO_FASTQTOBAM {
         --output ${prefix}.${suffix} \\
         ${sample_name} \\
         ${library_name}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
     """
 
     stub:
-    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def suffix = task.ext.suffix ?: "bam"
-
     """
     touch ${prefix}.${suffix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/fgbio/fastqtobam/meta.yml b/modules/nf-core/fgbio/fastqtobam/meta.yml
index bce76cf8c0..89d67a9f30 100644
--- a/modules/nf-core/fgbio/fastqtobam/meta.yml
+++ b/modules/nf-core/fgbio/fastqtobam/meta.yml
@@ -7,8 +7,8 @@ keywords:
   - cram
 tools:
   - fgbio:
-      description: A set of tools for working with genomic and high throughput sequencing
-        data, including UMIs
+      description: A set of tools for working with genomic and high throughput
+        sequencing data, including UMIs
       homepage: http://fulcrumgenomics.github.io/fgbio/
       documentation: http://fulcrumgenomics.github.io/fgbio/tools/latest/
       tool_dev_url: https://github.com/fulcrumgenomics/fgbio
@@ -24,32 +24,42 @@ input:
         type: file
         description: pair of reads to be converted into BAM file
         pattern: "*.{fastq.gz}"
+        ontologies: []
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.bam":
+      - "*.{bam,cram}":
           type: file
-          description: Unaligned, unsorted BAM file
-          pattern: "*.{bam}"
-  - cram:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.cram":
-          type: file
-          description: Unaligned, unsorted CRAM file
-          pattern: "*.{cram}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: Unaligned, unsorted BAM or CRAM file
+          pattern: "*.{bam,cram}"
+          ontologies: []
+  versions_fgbio:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@lescai"
   - "@matthdsm"
diff --git a/modules/nf-core/fgbio/groupreadsbyumi/environment.yml b/modules/nf-core/fgbio/groupreadsbyumi/environment.yml
index 4ebc0924d7..6af3be6aa4 100644
--- a/modules/nf-core/fgbio/groupreadsbyumi/environment.yml
+++ b/modules/nf-core/fgbio/groupreadsbyumi/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::fgbio=2.4.0
+  - bioconda::fgbio=3.1.2
diff --git a/modules/nf-core/fgbio/groupreadsbyumi/main.nf b/modules/nf-core/fgbio/groupreadsbyumi/main.nf
index c0506c9022..6a72ef3d79 100644
--- a/modules/nf-core/fgbio/groupreadsbyumi/main.nf
+++ b/modules/nf-core/fgbio/groupreadsbyumi/main.nf
@@ -1,20 +1,21 @@
 process FGBIO_GROUPREADSBYUMI {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/87/87626ef674e2f19366ae6214575a114fe80ce598e796894820550731706a84be/data' :
-        'community.wave.seqera.io/library/fgbio:2.4.0--913bad9d47ff8ddc' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4d/4d1150a2e123f49f8c268f0ab429847afae642376fa52af713b846b084df4a9f/data'
+        : 'community.wave.seqera.io/library/fgbio:3.1.2--6e9400d507a9dc55'}"
 
     input:
     tuple val(meta), path(bam)
-    val(strategy)
+    val strategy
 
     output:
-    tuple val(meta), path("*.bam")         , emit: bam
+    tuple val(meta), path("*.bam"), emit: bam
     tuple val(meta), path("*histogram.txt"), emit: histogram
-    path "versions.yml"                    , emit: versions
+    tuple val(meta), path("*read-metrics.txt"), emit: read_metrics
+    tuple val("${task.process}"), val('fgbio'), eval('fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\\[.*$//"'), topic: versions, emit: versions_fgbio
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,44 +25,42 @@ process FGBIO_GROUPREADSBYUMI {
     def prefix = task.ext.prefix ?: "${meta.id}_umi-grouped"
     def mem_gb = 8
     if (!task.memory) {
-        log.info '[fgbio FilterConsensusReads] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.'
-    } else if (mem_gb > task.memory.giga) {
+        log.info('[fgbio FilterConsensusReads] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.')
+    }
+    else if (mem_gb > task.memory.giga) {
         if (task.memory.giga < 2) {
             mem_gb = 1
-        } else {
+        }
+        else {
             mem_gb = task.memory.giga - 1
         }
     }
 
-    if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${bam}" == "${prefix}.bam") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
 
     """
     fgbio \\
         -Xmx${mem_gb}g \\
         --tmp-dir=. \\
         GroupReadsByUmi \\
-        -s $strategy \\
-        $args \\
-        -i $bam \\
+        -s ${strategy} \\
+        ${args} \\
+        -i ${bam} \\
         -o ${prefix}.bam \\
-        -f ${prefix}_histogram.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
+        -f ${prefix}_histogram.txt \\
+        --grouping-metrics ${prefix}_read-metrics.txt
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}_umi-grouped"
-    if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${bam}" == "${prefix}.bam") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
     """
     touch ${prefix}.bam
     touch ${prefix}_histogram.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
-    END_VERSIONS
+    touch ${prefix}_read-metrics.txt
     """
 }
diff --git a/modules/nf-core/fgbio/groupreadsbyumi/meta.yml b/modules/nf-core/fgbio/groupreadsbyumi/meta.yml
index c6d588daf1..34d38d7693 100644
--- a/modules/nf-core/fgbio/groupreadsbyumi/meta.yml
+++ b/modules/nf-core/fgbio/groupreadsbyumi/meta.yml
@@ -30,15 +30,16 @@ input:
         description: |
           BAM file. Note: the MQ tag is required on reads with mapped mates (!)
         pattern: "*.bam"
-  - - strategy:
-        type: string
-        enum: ["Identity", "Edit", "Adjacency", "Paired"]
-        description: |
-          Required argument: defines the UMI assignment strategy.
-          Must be chosen among: Identity, Edit, Adjacency, Paired.
+        ontologies: []
+  - strategy:
+      type: string
+      enum: ["Identity", "Edit", "Adjacency", "Paired"]
+      description: |
+        Required argument: defines the UMI assignment strategy.
+        Must be chosen among: Identity, Edit, Adjacency, Paired.
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -47,8 +48,9 @@ output:
           type: file
           description: UMI-grouped BAM
           pattern: "*.bam"
-  - histogram:
-      - meta:
+          ontologies: []
+  histogram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -57,11 +59,40 @@ output:
           type: file
           description: A text file containing the tag family size counts
           pattern: "*.txt"
-  - versions:
-      - versions.yml:
+          ontologies: []
+  read_metrics:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'test', single_end:false ]
+      - "*read-metrics.txt":
           type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          description: A text file containing the read count metrics from grouping
+          pattern: "*.txt"
+          ontologies: []
+  versions_fgbio:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - fgbio:
+          type: string
+          description: The tool name
+      - 'fgbio --version 2>&1 | tr -d "[:cntrl:]" | sed -e "s/^.*Version: //;s/\[.*$//"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@lescai"
 maintainers:
diff --git a/modules/nf-core/gawk/environment.yml b/modules/nf-core/gawk/environment.yml
index f52109e83b..185a0f5452 100644
--- a/modules/nf-core/gawk/environment.yml
+++ b/modules/nf-core/gawk/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::gawk=5.3.0
+  - conda-forge::gawk=5.3.1
diff --git a/modules/nf-core/gawk/main.nf b/modules/nf-core/gawk/main.nf
index 615b2ce923..3b9447301b 100644
--- a/modules/nf-core/gawk/main.nf
+++ b/modules/nf-core/gawk/main.nf
@@ -3,9 +3,9 @@ process GAWK {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/gawk:5.3.0' :
-        'biocontainers/gawk:5.3.0' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/a1/a125c778baf3865331101a104b60d249ee15fe1dca13bdafd888926cc5490a34/data' :
+        'community.wave.seqera.io/library/gawk:5.3.1--e09efb5dfc4b8156' }"
 
     input:
     tuple val(meta), path(input, arity: '0..*')
@@ -14,7 +14,7 @@ process GAWK {
 
     output:
     tuple val(meta), path("*.${suffix}"), emit: output
-    path "versions.yml"                 , emit: versions
+    tuple val("${task.process}"), val('gawk'), eval("awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//'"), topic: versions, emit: versions_gawk
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,18 +23,18 @@ process GAWK {
     def args  = task.ext.args  ?: '' // args is used for the main arguments of the tool
     def args2 = task.ext.args2 ?: '' // args2 is used to specify a program when no program file has been given
     prefix    = task.ext.prefix ?: "${meta.id}"
-    suffix    = task.ext.suffix ?: "${input.collect{ it.getExtension()}.get(0)}" // use the first extension of the input files
+    suffix    = task.ext.suffix ?: "${input.collect{ file -> file.getExtension()}.get(0)}" // use the first extension of the input files
 
     program    = program_file ? "-f ${program_file}" : "${args2}"
-    lst_gz     = input.findResults{ it.getExtension().endsWith("gz") ? it.toString() : null }
+    lst_gz     = input.findResults{ file -> file.getExtension().endsWith("gz") ? file.toString() : null }
     unzip      = lst_gz ? "gunzip -q -f ${lst_gz.join(" ")}" : ""
-    input_cmd  = input.collect { it.toString() - ~/\.gz$/ }.join(" ")
+    input_cmd  = input.collect { file -> file.toString() - ~/\.gz$/ }.join(" ")
     output_cmd = suffix.endsWith("gz") ? "| gzip > ${prefix}.${suffix}" : "> ${prefix}.${suffix}"
     output     = disable_redirect_output ? "" : output_cmd
-    cleanup    = lst_gz ? "rm ${lst_gz.collect{ it - ~/\.gz$/ }.join(" ")}" : ""
+    cleanup    = lst_gz ? "rm ${lst_gz.collect{ file -> file - ~/\.gz$/ }.join(" ")}" : ""
 
-    input.collect{
-        assert it.name != "${prefix}.${suffix}" : "Input and output names are the same, set prefix in module configuration to disambiguate!"
+    input.collect{ file ->
+        assert file.name != "${prefix}.${suffix}" : "Input and output names are the same, set prefix in module configuration to disambiguate!"
     }
 
     """
@@ -47,24 +47,14 @@ process GAWK {
         ${output}
 
     ${cleanup}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-    END_VERSIONS
     """
 
     stub:
     prefix = task.ext.prefix ?: "${meta.id}"
-    suffix = task.ext.suffix ?: "${input.getExtension()}"
+    suffix = task.ext.suffix ?: "${input.collect{ file -> file.getExtension()}.get(0)}"
     def create_cmd = suffix.endsWith("gz") ? "echo '' | gzip >" : "touch"
 
     """
     ${create_cmd} ${prefix}.${suffix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gawk/meta.yml b/modules/nf-core/gawk/meta.yml
index 34c50b125c..96cd0c72bc 100644
--- a/modules/nf-core/gawk/meta.yml
+++ b/modules/nf-core/gawk/meta.yml
@@ -15,7 +15,8 @@ tools:
       homepage: "https://www.gnu.org/software/gawk/"
       documentation: "https://www.gnu.org/software/gawk/manual/"
       tool_dev_url: "https://www.gnu.org/prep/ftp.html"
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: ""
 input:
   - - meta:
@@ -25,38 +26,58 @@ input:
           e.g. [ id:'test', single_end:false ]
     - input:
         type: file
-        description: The input file - Specify the logic that needs to be executed on
-          this file on the `ext.args2` or in the program file.
-          If the files have a `.gz` extension, they will be unzipped using `zcat`.
+        description: The input file - Specify the logic that needs to be executed
+          on this file on the `ext.args2` or in the program file. If the files
+          have a `.gz` extension, they will be unzipped using `zcat`.
         pattern: "*"
-  - - program_file:
-        type: file
-        description: Optional file containing logic for awk to execute. If you don't
-          wish to use a file, you can use `ext.args2` to specify the logic.
-        pattern: "*"
-  - - disable_redirect_output:
-        type: boolean
-        description: Disable the redirection of awk output to a given file. This is
-          useful if you want to use awk's built-in redirect to write files instead
-          of the shell's redirect.
+        ontologies: []
+  - program_file:
+      type: file
+      description: Optional file containing logic for awk to execute. If you don't
+        wish to use a file, you can use `ext.args2` to specify the logic.
+      pattern: "*"
+      ontologies: []
+  - disable_redirect_output:
+      type: boolean
+      description: Disable the redirection of awk output to a given file. This is
+        useful if you want to use awk's built-in redirect to write files instead
+        of the shell's redirect.
 output:
-  - output:
-      - meta:
+  output:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.${suffix}":
           type: file
-          description: The output file - if using shell redirection, specify the name of this
-            file using `ext.prefix` and the extension using `ext.suffix`. Otherwise, ensure
-            the awk program produces files with the extension in `ext.suffix`.
+          description: The output file - if using shell redirection, specify the
+            name of this file using `ext.prefix` and the extension using
+            `ext.suffix`. Otherwise, ensure the awk program produces files with
+            the extension in `ext.suffix`.
           pattern: "*"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gawk:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@nvnieuwk"
 maintainers:
diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf
index 3ffc8e9264..6edffc5930 100644
--- a/modules/nf-core/gunzip/main.nf
+++ b/modules/nf-core/gunzip/main.nf
@@ -3,7 +3,7 @@ process GUNZIP {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/52/52ccce28d2ab928ab862e25aae26314d69c8e38bd41ca9431c67ef05221348aa/data'
         : 'community.wave.seqera.io/library/coreutils_grep_gzip_lbzip2_pruned:838ba80435a629f8'}"
 
@@ -12,15 +12,16 @@ process GUNZIP {
 
     output:
     tuple val(meta), path("${gunzip}"), emit: gunzip
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('gunzip'), eval('gunzip --version 2>&1 | head -1 | sed "s/^.*(gzip) //; s/ Copyright.*//"'), topic: versions, emit: versions_gunzip
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def extension = (archive.toString() - '.gz').tokenize('.')[-1]
-    def name = archive.toString() - '.gz' - ".${extension}"
+    def nameWithoutGz = archive.extension == 'gz' ? archive.baseName : archive.name
+	def extension = file(nameWithoutGz).extension
+	def name = file(nameWithoutGz).baseName
     def prefix = task.ext.prefix ?: name
     gunzip = prefix + ".${extension}"
     """
@@ -32,24 +33,15 @@ process GUNZIP {
         ${args} \\
         ${archive} \\
         > ${gunzip}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
     """
 
     stub:
-    def args = task.ext.args ?: ''
-    def extension = (archive.toString() - '.gz').tokenize('.')[-1]
-    def name = archive.toString() - '.gz' - ".${extension}"
+    def nameWithoutGz = archive.extension == 'gz' ? archive.baseName : archive.name
+	def extension = file(nameWithoutGz).extension
+	def name = file(nameWithoutGz).baseName
     def prefix = task.ext.prefix ?: name
     gunzip = prefix + ".${extension}"
     """
     touch ${gunzip}
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml
index 69d3102455..bba6b3ba70 100644
--- a/modules/nf-core/gunzip/meta.yml
+++ b/modules/nf-core/gunzip/meta.yml
@@ -21,21 +21,42 @@ input:
         type: file
         description: File to be compressed/uncompressed
         pattern: "*.*"
+        ontologies: []
 output:
-  - gunzip:
-      - meta:
+  gunzip:
+    - - meta:
           type: file
           description: Compressed/uncompressed file
           pattern: "*.*"
+          ontologies: []
       - ${gunzip}:
           type: file
           description: Compressed/uncompressed file
           pattern: "*.*"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_gunzip:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - gunzip:
+          type: string
+          description: The tool name
+      - gunzip --version 2>&1 | head -1 | sed "s/^.*(gzip) //; s/ Copyright.*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - gunzip:
+          type: string
+          description: The tool name
+      - gunzip --version 2>&1 | head -1 | sed "s/^.*(gzip) //; s/ Copyright.*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/spring/decompress/main.nf b/modules/nf-core/spring/decompress/main.nf
index 86ced26906..585fc57792 100644
--- a/modules/nf-core/spring/decompress/main.nf
+++ b/modules/nf-core/spring/decompress/main.nf
@@ -3,9 +3,9 @@ process SPRING_DECOMPRESS {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/spring:1.1.1--h4ac6f70_2' :
-        'biocontainers/spring:1.1.1--h4ac6f70_2' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f6/f67f27c8cb2d1a149564f1a10f5f2b7a6acfa87ef3d3d27d2d8752dbe95e6acf/data' :
+        'community.wave.seqera.io/library/spring:1.1.1--911a17b4ccfb85ee' }"
 
     input:
     tuple val(meta), path(spring)
@@ -13,7 +13,8 @@ process SPRING_DECOMPRESS {
 
     output:
     tuple val(meta), path("*.fastq.gz"), emit: fastq
-    path "versions.yml"                , emit: versions
+    tuple val("${task.process}"), val('spring'), val('1.1.1'), topic: versions, emit: versions_spring
+    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
     when:
     task.ext.when == null || task.ext.when
@@ -21,9 +22,7 @@ process SPRING_DECOMPRESS {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '1.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     def output = write_one_fastq_gz ? "-o ${prefix}.fastq.gz" : "-o ${prefix}_R1.fastq.gz ${prefix}_R2.fastq.gz"
-
     """
     spring \\
         -d \\
@@ -32,23 +31,12 @@ process SPRING_DECOMPRESS {
         $args \\
         -i ${spring} \\
         ${output}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        spring: ${VERSION}
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def VERSION = '1.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
     def output = write_one_fastq_gz ? "echo '' | gzip > ${prefix}.fastq.gz" : "echo '' | gzip > ${prefix}_R1.fastq.gz; echo '' | gzip > ${prefix}_R2.fastq.gz"
     """
     ${output}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        spring: ${VERSION}
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/spring/decompress/meta.yml b/modules/nf-core/spring/decompress/meta.yml
index 72b72b75da..e41c4bf901 100644
--- a/modules/nf-core/spring/decompress/meta.yml
+++ b/modules/nf-core/spring/decompress/meta.yml
@@ -24,14 +24,15 @@ input:
         type: file
         description: Spring file to decompress.
         pattern: "*.{spring}"
-  - - write_one_fastq_gz:
-        type: boolean
-        description: |
-          Controls whether spring should write one fastq.gz file with reads from both directions or two fastq.gz files with reads from distinct directions
-        pattern: "true or false"
+        ontologies: []
+  - write_one_fastq_gz:
+      type: boolean
+      description: |
+        Controls whether spring should write one fastq.gz file with reads from both directions or two fastq.gz files with reads from distinct directions
+      pattern: "true or false"
 output:
-  - fastq:
-      - meta:
+  fastq:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -40,11 +41,28 @@ output:
           type: file
           description: Decompressed FASTQ file(s).
           pattern: "*.{fastq.gz}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_spring:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - spring:
+          type: string
+          description: The name of the tool
+      - 1.1.1:
+          type: string
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - spring:
+          type: string
+          description: The name of the tool
+      - 1.1.1:
+          type: string
+          description: The expression to obtain the version of the tool
 authors:
   - "@xec-cm"
 maintainers:
diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf
index e712ebe63a..bf2c056c62 100644
--- a/modules/nf-core/untar/main.nf
+++ b/modules/nf-core/untar/main.nf
@@ -3,7 +3,7 @@ process UNTAR {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/52/52ccce28d2ab928ab862e25aae26314d69c8e38bd41ca9431c67ef05221348aa/data'
         : 'community.wave.seqera.io/library/coreutils_grep_gzip_lbzip2_pruned:838ba80435a629f8'}"
 
@@ -12,7 +12,7 @@ process UNTAR {
 
     output:
     tuple val(meta), path("${prefix}"), emit: untar
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('untar'), eval('tar --version 2>&1 | head -1 | sed "s/tar (GNU tar) //; s/ Copyright.*//"'), emit: versions_untar, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -43,10 +43,6 @@ process UNTAR {
             ${args2}
     fi
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -75,10 +71,5 @@ process UNTAR {
             fi
         done
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml
index 1b6bf491e6..571d8078a4 100644
--- a/modules/nf-core/untar/meta.yml
+++ b/modules/nf-core/untar/meta.yml
@@ -1,5 +1,5 @@
 name: untar
-description: Extract files.
+description: Extract files from tar, tar.gz, tar.bz2, tar.xz archives
 keywords:
   - untar
   - uncompress
@@ -7,7 +7,7 @@ keywords:
 tools:
   - untar:
       description: |
-        Extract tar.gz files.
+        Extract tar, tar.gz, tar.bz2, tar.xz files.
       documentation: https://www.gnu.org/software/tar/manual/
       licence: ["GPL-3.0-or-later"]
       identifier: ""
@@ -19,8 +19,8 @@ input:
           e.g. [ id:'test', single_end:false ]
     - archive:
         type: file
-        description: File to be untar
-        pattern: "*.{tar}.{gz}"
+        description: File to be untarred
+        pattern: "*.{tar,tar.gz,tar.bz2,tar.xz}"
         ontologies:
           - edam: http://edamontology.org/format_3981 # TAR format
           - edam: http://edamontology.org/format_3989 # GZIP format
@@ -38,13 +38,29 @@ output:
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
           pattern: "*/"
+  versions_untar:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - untar:
+          type: string
+          description: The name of the tool
+      - tar --version 2>&1 | head -1 | sed "s/tar (GNU tar) //; s/ Copyright.*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - untar:
+          type: string
+          description: The name of the tool
+      - tar --version 2>&1 | head -1 | sed "s/tar (GNU tar) //; s/ Copyright.*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@joseespinosa"
   - "@drpatelh"
diff --git a/modules/nf-core/unzip/main.nf b/modules/nf-core/unzip/main.nf
index a0c02109cd..eb020ba13f 100644
--- a/modules/nf-core/unzip/main.nf
+++ b/modules/nf-core/unzip/main.nf
@@ -3,16 +3,16 @@ process UNZIP {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/p7zip:16.02' :
-        'biocontainers/p7zip:16.02' }"
+        'quay.io/biocontainers/p7zip:16.02' }"
 
     input:
     tuple val(meta), path(archive)
 
     output:
     tuple val(meta), path("${prefix}/"), emit: unzipped_archive
-    path "versions.yml"                , emit: versions
+    tuple val("${task.process}"), val('7za'), eval("7za 2>&1 | sed -n '2s/^.* \\([0-9.]*\\) .*/\\1/p'"), topic: versions, emit: versions_7za
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,23 +27,12 @@ process UNZIP {
         -o"${prefix}"/ \\
         $args \\
         $archive
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        7za: \$(echo \$(7za --help) | sed 's/.*p7zip Version //; s/(.*//')
-    END_VERSIONS
     """
 
     stub:
-    def args = task.ext.args ?: ''
     if ( archive instanceof List && archive.name.size > 1 ) { error "[UNZIP] error: 7za only accepts a single archive as input. Please check module input." }
     prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName)
     """
     mkdir "${prefix}"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        7za: \$(echo \$(7za --help) | sed 's/.*p7zip Version //; s/(.*//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/unzip/meta.yml b/modules/nf-core/unzip/meta.yml
index ba1eb9129b..1c645556f7 100644
--- a/modules/nf-core/unzip/meta.yml
+++ b/modules/nf-core/unzip/meta.yml
@@ -7,12 +7,13 @@ keywords:
   - archiving
 tools:
   - unzip:
-      description: p7zip is a quick port of 7z.exe and 7za.exe (command line version
-        of 7zip, see www.7-zip.org) for Unix.
+      description: p7zip is a quick port of 7z.exe and 7za.exe (command line
+        version of 7zip, see www.7-zip.org) for Unix.
       homepage: https://sourceforge.net/projects/p7zip/
       documentation: https://sourceforge.net/projects/p7zip/
       tool_dev_url: https://sourceforge.net/projects/p7zip"
-      licence: ["LGPL-2.1-or-later"]
+      licence:
+        - "LGPL-2.1-or-later"
       identifier: ""
 input:
   - - meta:
@@ -25,7 +26,7 @@ input:
         description: ZIP file
         pattern: "*.zip"
         ontologies:
-          - edam: http://edamontology.org/format_3987 # ZIP format
+          - edam: http://edamontology.org/format_3987
 output:
   unzipped_archive:
     - - meta:
@@ -37,13 +38,27 @@ output:
           type: directory
           description: Directory contents of the unzipped archive
           pattern: "${archive.baseName}/"
+  versions_7za:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - 7za:
+          type: string
+          description: The name of the tool
+      - 7za 2>&1 | sed -n '2s/^.* \([0-9.]*\) .*/\1/p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - 7za:
+          type: string
+          description: The name of the tool
+      - 7za 2>&1 | sed -n '2s/^.* \([0-9.]*\) .*/\1/p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@jfy133"
 maintainers:
diff --git a/subworkflows/local/bam_convert_samtools/main.nf b/subworkflows/local/bam_convert_samtools/main.nf
index d101f34b45..56ee28f72b 100644
--- a/subworkflows/local/bam_convert_samtools/main.nf
+++ b/subworkflows/local/bam_convert_samtools/main.nf
@@ -60,7 +60,6 @@ workflow BAM_CONVERT_SAMTOOLS {
     reads = CAT_FASTQ.out.reads
 
     // Gather versions of all tools used
-    versions = versions.mix(CAT_FASTQ.out.versions)
     versions = versions.mix(COLLATE_FASTQ_MAP.out.versions)
     versions = versions.mix(COLLATE_FASTQ_UNMAP.out.versions)
     versions = versions.mix(SAMTOOLS_MERGE_UNMAP.out.versions)
diff --git a/subworkflows/local/bam_variant_calling_mpileup/main.nf b/subworkflows/local/bam_variant_calling_mpileup/main.nf
index d46aa77b1b..125a4a4684 100644
--- a/subworkflows/local/bam_variant_calling_mpileup/main.nf
+++ b/subworkflows/local/bam_variant_calling_mpileup/main.nf
@@ -75,7 +75,6 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
         .map { meta, tbi -> [meta - meta.subMap('num_intervals') + [variantcaller: 'bcftools'], tbi] }
 
     versions = versions.mix(SAMTOOLS_MPILEUP.out.versions)
-    versions = versions.mix(CAT_MPILEUP.out.versions)
 
     emit:
     mpileup
diff --git a/subworkflows/local/fastq_align/main.nf b/subworkflows/local/fastq_align/main.nf
index f6e5f8a6ec..2c711a8259 100644
--- a/subworkflows/local/fastq_align/main.nf
+++ b/subworkflows/local/fastq_align/main.nf
@@ -19,7 +19,6 @@ workflow FASTQ_ALIGN {
 
     main:
 
-    versions = channel.empty()
     reports = channel.empty()
 
     // Only one of the following should be run
@@ -42,13 +41,8 @@ workflow FASTQ_ALIGN {
     // Gather reports of all tools used
     reports = reports.mix(DRAGMAP_ALIGN.out.log)
 
-    // Gather versions of all tools used
-    versions = versions.mix(BWAMEM1_MEM.out.versions)
-    versions = versions.mix(BWAMEM2_MEM.out.versions)
-    versions = versions.mix(DRAGMAP_ALIGN.out.versions)
     emit:
     bam      // channel: [ [meta], bam ]
     bai      // channel: [ [meta], bai ]
     reports
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf b/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
index 68851c58b7..f173a52108 100644
--- a/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
+++ b/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
@@ -73,10 +73,6 @@ workflow FASTQ_CREATE_UMI_CONSENSUS_FGBIO {
     CALLUMICONSENSUS(GROUPREADSBYUMI.out.bam, call_min_reads, call_min_baseq)
 
     ch_versions = ch_versions.mix(BAM2FASTQ.out.versions)
-    ch_versions = ch_versions.mix(ALIGN_UMI.out.versions)
-    ch_versions = ch_versions.mix(CALLUMICONSENSUS.out.versions)
-    ch_versions = ch_versions.mix(FASTQTOBAM.out.versions)
-    ch_versions = ch_versions.mix(GROUPREADSBYUMI.out.versions)
     ch_versions = ch_versions.mix(MERGE_CONSENSUS.out.versions)
 
     emit:
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index cbce80043e..df793c1de8 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -109,8 +109,7 @@ workflow FASTQ_PREPROCESS_GATK {
             save_trimmed_fail = false
             save_merged = false
             FASTP(
-                reads_for_fastp,
-                [], // we are not using any adapter fastas at the moment
+                reads_for_fastp.map { meta, reads -> [ meta, reads, [] ] }, // adapter_fasta folded into the reads tuple (unused)
                 false, // we don't use discard_trimmed_pass at the moment
                 save_trimmed_fail,
                 save_merged
@@ -126,7 +125,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 }.transpose()
             } else reads_for_bbsplit = FASTP.out.reads
 
-            versions = versions.mix(FASTP.out.versions)
 
         } else {
             reads_for_bbsplit = reads_for_fastp
@@ -182,7 +180,6 @@ workflow FASTQ_PREPROCESS_GATK {
             FGBIO_COPYUMIFROMREADNAME(FASTQ_ALIGN.out.bam.map{meta, bam -> [meta, bam, []]})
             aligned_bam = FGBIO_COPYUMIFROMREADNAME.out.bam
             aligned_bai = FGBIO_COPYUMIFROMREADNAME.out.bai
-            versions = versions.mix(FGBIO_COPYUMIFROMREADNAME.out.versions)
         } else {
             aligned_bam = FASTQ_ALIGN.out.bam
             aligned_bai = FASTQ_ALIGN.out.bai
@@ -250,8 +247,6 @@ workflow FASTQ_PREPROCESS_GATK {
             versions = versions.mix(BAM_TO_CRAM_MAPPING.out.versions)
         }
 
-        // Gather used softwares versions
-        versions = versions.mix(FASTQ_ALIGN.out.versions)
     }
 
     if (params.step in ['mapping', 'markduplicates']) {
@@ -270,7 +265,6 @@ workflow FASTQ_PREPROCESS_GATK {
         if(params.step == 'markduplicates' && params.umi_in_read_header) {
             FGBIO_COPYUMIFROMREADNAME(cram_for_markduplicates.map{ meta, bam -> [ meta, bam, [] ] })
             cram_for_markduplicates = FGBIO_COPYUMIFROMREADNAME.out.bam
-            versions = versions.mix(FGBIO_COPYUMIFROMREADNAME.out.versions)
         }
 
         // if no MD is done, then run QC on mapped & converted CRAM files
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 195355676e..5193807d1d 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -64,7 +64,6 @@ workflow PREPARE_GENOME {
         if (!bwa_in && (aligner == "bwa-mem" || aligner == "sentieon-bwamem" || aligner == "parabricks")) {
             BWAMEM1_INDEX(fasta)
             index_alignment = BWAMEM1_INDEX.out.index.collect()
-            versions = versions.mix(BWAMEM1_INDEX.out.versions)
         }
         else if (aligner == "bwa-mem" || aligner == "sentieon-bwamem" || aligner == "parabricks") {
             index_alignment = channel.fromPath(bwa_in).map { index -> [[id: 'bwa'], index] }.collect()
@@ -72,7 +71,6 @@ workflow PREPARE_GENOME {
         else if (!bwamem2_in && aligner == 'bwa-mem2') {
             BWAMEM2_INDEX(fasta)
             index_alignment = BWAMEM2_INDEX.out.index.collect()
-            versions = versions.mix(BWAMEM2_INDEX.out.versions)
         }
         else if (aligner == 'bwa-mem2') {
             index_alignment = channel.fromPath(bwamem2_in).map { index -> [[id: 'bwamem2'], index] }.collect()
@@ -80,7 +78,6 @@ workflow PREPARE_GENOME {
         else if (!dragmap_in && aligner == 'dragmap') {
             DRAGMAP_HASHTABLE(fasta)
             index_alignment = DRAGMAP_HASHTABLE.out.hashmap.collect()
-            versions = versions.mix(DRAGMAP_HASHTABLE.out.versions)
         }
         else if (aligner == 'dragmap') {
             index_alignment = channel.fromPath(dragmap_in).map { index -> [[id: 'dragmap'], index] }.collect()
@@ -120,7 +117,6 @@ workflow PREPARE_GENOME {
             // Use user-provided bbsplit index
             if (bbsplit_index_in.endsWith('.tar.gz')) {
                 bbsplit_index = UNTAR_BBSPLIT_INDEX([[id: 'bbsplit_index'], file(bbsplit_index_in, checkIfExists: true)]).untar.map { _meta, index -> index }
-                versions = versions.mix(UNTAR_BBSPLIT_INDEX.out.versions)
             }
             else {
                 bbsplit_index = channel.value(file(bbsplit_index_in, checkIfExists: true))
@@ -210,7 +206,6 @@ workflow PREPARE_GENOME {
     if (msisensor2_models_in && msisensor2_models_in.endsWith(".tar.gz") && tools.split(',').contains('msisensor2')) {
         UNTAR_MSISENSOR2_MODELS(channel.fromPath(file(msisensor2_models_in)).map { archive -> [[id: archive.baseName], archive] })
         msisensor2_models = UNTAR_MSISENSOR2_MODELS.out.untar.collect()
-        versions = versions.mix(UNTAR_MSISENSOR2_MODELS.out.versions)
     }
     else if (msisensor2_models_in && tools.split(',').contains('msisensor2')) {
         msisensor2_models = channel.fromPath(msisensor2_models_in).map { model -> [[id:model.baseName], model] }.collect()
@@ -238,7 +233,6 @@ workflow PREPARE_GENOME {
     else if (ascat_alleles_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
         UNZIP_ALLELES(channel.fromPath(file(ascat_alleles_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_alleles = UNZIP_ALLELES.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
-        versions = versions.mix(UNZIP_ALLELES.out.versions)
     }
     else {
         ascat_alleles = channel.fromPath(ascat_alleles_in).collect()
@@ -250,7 +244,6 @@ workflow PREPARE_GENOME {
     else if (ascat_loci_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
         UNZIP_LOCI(channel.fromPath(file(ascat_loci_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci = UNZIP_LOCI.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
-        versions = versions.mix(UNZIP_LOCI.out.versions)
     }
     else {
         ascat_loci = channel.fromPath(ascat_loci_in).collect()
@@ -262,7 +255,6 @@ workflow PREPARE_GENOME {
     else if (ascat_loci_gc_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
         UNZIP_GC(channel.fromPath(file(ascat_loci_gc_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci_gc = UNZIP_GC.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
-        versions = versions.mix(UNZIP_GC.out.versions)
     }
     else {
         ascat_loci_gc = channel.fromPath(ascat_loci_gc_in).collect()
@@ -274,7 +266,6 @@ workflow PREPARE_GENOME {
     else if (ascat_loci_rt_in.endsWith(".zip") && tools.split(',').contains('ascat')) {
         UNZIP_RT(channel.fromPath(file(ascat_loci_rt_in)).map { archive -> [[id: archive.baseName], archive] })
         ascat_loci_rt = UNZIP_RT.out.unzipped_archive.map { _meta, extracted_archive -> extracted_archive }.collect()
-        versions = versions.mix(UNZIP_RT.out.versions)
     }
     else {
         ascat_loci_rt = channel.fromPath(ascat_loci_rt_in).collect()
@@ -286,7 +277,6 @@ workflow PREPARE_GENOME {
     else if (chr_dir_in.endsWith(".tar.gz") && tools.split(',').contains('controlfreec')) {
         UNTAR_CHR_DIR(channel.fromPath(file(chr_dir_in)).map { archive -> [[id: archive.baseName], archive] })
         chr_dir = UNTAR_CHR_DIR.out.untar.map { _meta, extracted_archive -> extracted_archive }.collect()
-        versions = versions.mix(UNTAR_CHR_DIR.out.versions)
     }
     else {
         chr_dir = channel.fromPath(chr_dir_in).collect()
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index 6973896efb..55bf2e233a 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -47,7 +47,6 @@ workflow PREPARE_INTERVALS {
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
 
-            versions = versions.mix(BUILD_INTERVALS.out.versions)
             versions = versions.mix(CREATE_INTERVALS_BED.out.versions)
         } else {
             intervals_combined = channel.fromPath(file(intervals)).map{bed -> [ [ id:bed.baseName ], bed ] }
diff --git a/tests/aligner-bwa-mem.nf.test.snap b/tests/aligner-bwa-mem.nf.test.snap
index fb1f52fe06..0ca5918bbd 100644
--- a/tests/aligner-bwa-mem.nf.test.snap
+++ b/tests/aligner-bwa-mem.nf.test.snap
@@ -7,11 +7,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -90,7 +90,7 @@
             5,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -141,7 +141,7 @@
             5,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -207,11 +207,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/aligner-bwa-mem2.nf.test.snap b/tests/aligner-bwa-mem2.nf.test.snap
index d03b12f392..da63c14f1b 100644
--- a/tests/aligner-bwa-mem2.nf.test.snap
+++ b/tests/aligner-bwa-mem2.nf.test.snap
@@ -74,7 +74,7 @@
                 },
                 "BWAMEM2_MEM": {
                     "bwamem2": "2.2.1",
-                    "samtools": 1.21
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -211,7 +211,7 @@
                 },
                 "BWAMEM2_MEM": {
                     "bwamem2": "2.2.1",
-                    "samtools": 1.21
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index 1cd537c5ed..bcd4637927 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -4,7 +4,7 @@
             15,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": 1.21
@@ -237,7 +237,7 @@
             14,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -476,7 +476,7 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CALCULATECONTAMINATION": {
                     "gatk4": "4.6.2.0"
@@ -797,7 +797,7 @@
             13,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/alignment_from_everything.nf.test.snap b/tests/alignment_from_everything.nf.test.snap
index a01f7e60cc..14e2c4a9d6 100644
--- a/tests/alignment_from_everything.nf.test.snap
+++ b/tests/alignment_from_everything.nf.test.snap
@@ -4,14 +4,14 @@
             61,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CAT_FASTQ": {
-                    "cat": 9.5
+                    "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
                     "samtools": 1.21
diff --git a/tests/alignment_to_fastq.nf.test.snap b/tests/alignment_to_fastq.nf.test.snap
index 605d3e424f..7511faf85a 100644
--- a/tests/alignment_to_fastq.nf.test.snap
+++ b/tests/alignment_to_fastq.nf.test.snap
@@ -4,14 +4,14 @@
             25,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CAT_FASTQ": {
-                    "cat": 9.5
+                    "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
                     "samtools": 1.21
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index 28b1cfe2b9..f465be1784 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -13,11 +13,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -377,11 +377,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -601,14 +601,14 @@
                     "bbmap": "39.18"
                 },
                 "BUILD_INTERVALS": {
-                    "gawk": "5.3.0"
+                    "gawk": "5.3.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index 4a3e6a3bf0..c578704c66 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -7,11 +7,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -334,11 +334,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/fastp.nf.test.snap b/tests/fastp.nf.test.snap
index 25e72cefa1..111b6110ba 100644
--- a/tests/fastp.nf.test.snap
+++ b/tests/fastp.nf.test.snap
@@ -4,17 +4,17 @@
             20,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "1.1.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -235,10 +235,10 @@
                 "preprocessing",
                 "preprocessing/fastp",
                 "preprocessing/fastp/test",
-                "preprocessing/fastp/test/test-test_L1_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/test-test_L1_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/test-test_L2_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/test-test_L2_2.fastp.fastq.gz",
+                "preprocessing/fastp/test/test-test_L1_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/test-test_L1_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/test-test_L2_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/test-test_L2_R2.fastp.fastq.gz",
                 "preprocessing/markduplicates",
                 "preprocessing/markduplicates/test",
                 "preprocessing/markduplicates/test/test.md.cram",
@@ -320,7 +320,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,d97ac04e524b20bf9d261c009ac92b6a",
                 "mosdepth_perchrom.txt:md5,29af8f8744a88e2ec0486292d541559f",
                 "multiqc_citations.txt:md5,0e2971e7a873c92592112775fa99fb02",
-                "multiqc_fastp.txt:md5,2f22f6c961e432974f27a4e56d877cec",
+                "multiqc_fastp.txt:md5,39b27871814457c42ea33aa131b8ecfc",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
                 "picard_MarkIlluminaAdapters_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -329,12 +329,12 @@
                 "samtools-stats-dp.txt:md5,58ea8c88565a359af678aa588e44895c",
                 "samtools_alignment_plot.txt:md5,aac925e42c9da438ab9f0fb7ae77e2fa",
                 "samtools_insert_size.txt:md5,9eccc96ce006c0161497782ed3afc147",
-                "test-test_L1_1.fastp.fastq.gz:md5,f1a5c524cae7be9b5ca9a4138f847cfa",
-                "test-test_L1_2.fastp.fastq.gz:md5,e366994a1db55b5ed3cd12482f33cee7",
-                "test-test_L2_1.fastp.fastq.gz:md5,f1a5c524cae7be9b5ca9a4138f847cfa",
-                "test-test_L2_2.fastp.fastq.gz:md5,e366994a1db55b5ed3cd12482f33cee7",
-                "test-test_L1.fastp.json:md5,fb88c7b5807f6c7478b01baddf8ca4e8",
-                "test-test_L2.fastp.json:md5,708187bd90c12b1c8c3fa7046b69dc35",
+                "test-test_L1_R1.fastp.fastq.gz:md5,f1a5c524cae7be9b5ca9a4138f847cfa",
+                "test-test_L1_R2.fastp.fastq.gz:md5,e366994a1db55b5ed3cd12482f33cee7",
+                "test-test_L2_R1.fastp.fastq.gz:md5,f1a5c524cae7be9b5ca9a4138f847cfa",
+                "test-test_L2_R2.fastp.fastq.gz:md5,e366994a1db55b5ed3cd12482f33cee7",
+                "test-test_L1.fastp.json:md5,80fa7cc86ef9fa02eeb9c9d25c6f2672",
+                "test-test_L2.fastp.json:md5,138a8dbd84d89dcf19c303fdc05754e9",
                 "test.md.mosdepth.global.dist.txt:md5,b1c26e3381f220e65d683048ab6b6e2a",
                 "test.md.mosdepth.region.dist.txt:md5,02d51752367e753a6984c12f059499ba",
                 "test.md.mosdepth.summary.txt:md5,f18e776c3ee8e6947c3a69c136f54860",
@@ -367,17 +367,17 @@
             26,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "1.1.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -598,22 +598,22 @@
                 "preprocessing",
                 "preprocessing/fastp",
                 "preprocessing/fastp/test",
-                "preprocessing/fastp/test/0001.test-test_L1_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0001.test-test_L1_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0001.test-test_L2_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0001.test-test_L2_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0002.test-test_L1_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0002.test-test_L1_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0002.test-test_L2_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0002.test-test_L2_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0003.test-test_L1_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0003.test-test_L1_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0003.test-test_L2_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0003.test-test_L2_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0004.test-test_L1_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0004.test-test_L1_2.fastp.fastq.gz",
-                "preprocessing/fastp/test/0004.test-test_L2_1.fastp.fastq.gz",
-                "preprocessing/fastp/test/0004.test-test_L2_2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0001.test-test_L1_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0001.test-test_L1_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0001.test-test_L2_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0001.test-test_L2_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0002.test-test_L1_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0002.test-test_L1_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0002.test-test_L2_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0002.test-test_L2_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0003.test-test_L1_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0003.test-test_L1_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0003.test-test_L2_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0003.test-test_L2_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0004.test-test_L1_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0004.test-test_L1_R2.fastp.fastq.gz",
+                "preprocessing/fastp/test/0004.test-test_L2_R1.fastp.fastq.gz",
+                "preprocessing/fastp/test/0004.test-test_L2_R2.fastp.fastq.gz",
                 "preprocessing/markduplicates",
                 "preprocessing/markduplicates/test",
                 "preprocessing/markduplicates/test/test.md.cram",
@@ -695,7 +695,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,722e8c7583dd52b2c8d8bb923718c912",
                 "mosdepth_perchrom.txt:md5,b9304bb6dee33d25255e19693c0a4dd8",
                 "multiqc_citations.txt:md5,0e2971e7a873c92592112775fa99fb02",
-                "multiqc_fastp.txt:md5,758582c0fafe6e69ad8488eb612008de",
+                "multiqc_fastp.txt:md5,e5a4f17bb627d529e829f11fda3bddc3",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
                 "picard_MarkIlluminaAdapters_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -704,24 +704,24 @@
                 "samtools-stats-dp.txt:md5,93ec69f6db74d9de7e4a549491ae2c73",
                 "samtools_alignment_plot.txt:md5,962b7dd27f48ec4bbb668c8c32cbea1b",
                 "samtools_insert_size.txt:md5,555c4ca8aac120c3b23e6cd2457922cd",
-                "0001.test-test_L1_1.fastp.fastq.gz:md5,f379a60cc2a41f39fa0c0d23edf6eb61",
-                "0001.test-test_L1_2.fastp.fastq.gz:md5,e54d912158088d98f8083854079e1f5c",
-                "0001.test-test_L2_1.fastp.fastq.gz:md5,f379a60cc2a41f39fa0c0d23edf6eb61",
-                "0001.test-test_L2_2.fastp.fastq.gz:md5,e54d912158088d98f8083854079e1f5c",
-                "0002.test-test_L1_1.fastp.fastq.gz:md5,366180a220f6b78c55b1b23b35dc7f96",
-                "0002.test-test_L1_2.fastp.fastq.gz:md5,66ca650f883997310858acac94c8d236",
-                "0002.test-test_L2_1.fastp.fastq.gz:md5,366180a220f6b78c55b1b23b35dc7f96",
-                "0002.test-test_L2_2.fastp.fastq.gz:md5,66ca650f883997310858acac94c8d236",
-                "0003.test-test_L1_1.fastp.fastq.gz:md5,46bc09415a26d50230163e613cb1324b",
-                "0003.test-test_L1_2.fastp.fastq.gz:md5,500404ef12d7c6f02eb3b5367f84a978",
-                "0003.test-test_L2_1.fastp.fastq.gz:md5,46bc09415a26d50230163e613cb1324b",
-                "0003.test-test_L2_2.fastp.fastq.gz:md5,500404ef12d7c6f02eb3b5367f84a978",
-                "0004.test-test_L1_1.fastp.fastq.gz:md5,f3b1d77aa32f019f3088810cac2605e9",
-                "0004.test-test_L1_2.fastp.fastq.gz:md5,e38c0529e6ba0abce221ac39c84263b0",
-                "0004.test-test_L2_1.fastp.fastq.gz:md5,f3b1d77aa32f019f3088810cac2605e9",
-                "0004.test-test_L2_2.fastp.fastq.gz:md5,e38c0529e6ba0abce221ac39c84263b0",
-                "test-test_L1.fastp.json:md5,2f72ca9fa3c55cfd4ff67a747f7c2e46",
-                "test-test_L2.fastp.json:md5,f8b183e541878de7b24e555e23e078f1",
+                "0001.test-test_L1_R1.fastp.fastq.gz:md5,f379a60cc2a41f39fa0c0d23edf6eb61",
+                "0001.test-test_L1_R2.fastp.fastq.gz:md5,e54d912158088d98f8083854079e1f5c",
+                "0001.test-test_L2_R1.fastp.fastq.gz:md5,f379a60cc2a41f39fa0c0d23edf6eb61",
+                "0001.test-test_L2_R2.fastp.fastq.gz:md5,e54d912158088d98f8083854079e1f5c",
+                "0002.test-test_L1_R1.fastp.fastq.gz:md5,366180a220f6b78c55b1b23b35dc7f96",
+                "0002.test-test_L1_R2.fastp.fastq.gz:md5,66ca650f883997310858acac94c8d236",
+                "0002.test-test_L2_R1.fastp.fastq.gz:md5,366180a220f6b78c55b1b23b35dc7f96",
+                "0002.test-test_L2_R2.fastp.fastq.gz:md5,66ca650f883997310858acac94c8d236",
+                "0003.test-test_L1_R1.fastp.fastq.gz:md5,46bc09415a26d50230163e613cb1324b",
+                "0003.test-test_L1_R2.fastp.fastq.gz:md5,500404ef12d7c6f02eb3b5367f84a978",
+                "0003.test-test_L2_R1.fastp.fastq.gz:md5,46bc09415a26d50230163e613cb1324b",
+                "0003.test-test_L2_R2.fastp.fastq.gz:md5,500404ef12d7c6f02eb3b5367f84a978",
+                "0004.test-test_L1_R1.fastp.fastq.gz:md5,f3b1d77aa32f019f3088810cac2605e9",
+                "0004.test-test_L1_R2.fastp.fastq.gz:md5,e38c0529e6ba0abce221ac39c84263b0",
+                "0004.test-test_L2_R1.fastp.fastq.gz:md5,f3b1d77aa32f019f3088810cac2605e9",
+                "0004.test-test_L2_R2.fastp.fastq.gz:md5,e38c0529e6ba0abce221ac39c84263b0",
+                "test-test_L1.fastp.json:md5,34c54addc091d14c6c9582ef2ed16a85",
+                "test-test_L2.fastp.json:md5,607e27e248389ec266eecc847c6f9936",
                 "test.md.mosdepth.global.dist.txt:md5,e5d0c6bf323c32f5414bd48b90bb32fa",
                 "test.md.mosdepth.region.dist.txt:md5,5062f8b7bb536c9b77a68f4ccd2315c2",
                 "test.md.mosdepth.summary.txt:md5,455358d5943fd1e5f09853acff3e50b6",
@@ -754,17 +754,17 @@
             20,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "1.1.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1064,7 +1064,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,caee7b9e5d1a451970f87d791c3e450b",
                 "mosdepth_perchrom.txt:md5,a0990e98cdd2cd5540d07f504f516ccf",
                 "multiqc_citations.txt:md5,0e2971e7a873c92592112775fa99fb02",
-                "multiqc_fastp.txt:md5,2e4305ae77790e1948c19acc778b508c",
+                "multiqc_fastp.txt:md5,8c270b44aaec9ee1bd7bf19d2150a51e",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
                 "picard_MarkIlluminaAdapters_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -1073,8 +1073,8 @@
                 "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
-                "test-test_L1.fastp.json:md5,ced0232bc3be313b392a9f59ff970cc2",
-                "test-test_L2.fastp.json:md5,d05b922b85ecd0e087ba05af543f8ff8",
+                "test-test_L1.fastp.json:md5,a7746b05c3ffb374c8f5d204f971879e",
+                "test-test_L2.fastp.json:md5,d896569d921616f61a829b62477c1ffa",
                 "test.md.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
diff --git a/tests/intervals.nf.test.snap b/tests/intervals.nf.test.snap
index d7af934a8a..d4af6db508 100644
--- a/tests/intervals.nf.test.snap
+++ b/tests/intervals.nf.test.snap
@@ -7,14 +7,14 @@
                     "samtools": 1.21
                 },
                 "BUILD_INTERVALS": {
-                    "gawk": "5.3.0"
+                    "gawk": "5.3.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -91,11 +91,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -153,11 +153,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -211,11 +211,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -264,11 +264,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -321,14 +321,14 @@
                     "samtools": 1.21
                 },
                 "BUILD_INTERVALS": {
-                    "gawk": "5.3.0"
+                    "gawk": "5.3.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 794d5c6ed3..6721c744eb 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -238,7 +238,7 @@
                     "bcftools": "1.23.1"
                 },
                 "BUILD_INTERVALS": {
-                    "gawk": "5.3.0"
+                    "gawk": "5.3.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/lane_integer.nf.test.snap b/tests/lane_integer.nf.test.snap
index 19bd1a0e5f..e6d0a7f91a 100644
--- a/tests/lane_integer.nf.test.snap
+++ b/tests/lane_integer.nf.test.snap
@@ -7,11 +7,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/multi_lane.nf.test.snap b/tests/multi_lane.nf.test.snap
index a9541143a4..334e9fbdbe 100644
--- a/tests/multi_lane.nf.test.snap
+++ b/tests/multi_lane.nf.test.snap
@@ -7,11 +7,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -125,17 +125,17 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CALLUMICONSENSUS": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "CAT_FASTQ": {
-                    "cat": 9.5
+                    "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
                     "samtools": 1.21
@@ -147,13 +147,13 @@
                     "gawk": "5.3.0"
                 },
                 "FASTQTOBAM": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_INTERVALLISTTOBED": {
                     "gatk4": "4.6.2.0"
                 },
                 "GROUPREADSBYUMI": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "INDEX_MERGE_BAM": {
                     "samtools": 1.21
@@ -203,16 +203,18 @@
                 "reference",
                 "reports",
                 "reports/umi",
-                "reports/umi/test_umi-grouped_histogram.txt"
+                "reports/umi/test_umi-grouped_histogram.txt",
+                "reports/umi/test_umi-grouped_read-metrics.txt"
             ],
             [
-                "test_umi-grouped_histogram.txt:md5,47440eca0cc1e70be22fe2a2ce81dfbf"
+                "test_umi-grouped_histogram.txt:md5,4e3bc8b3ed89ddf32ee3543af98f1033",
+                "test_umi-grouped_read-metrics.txt:md5,c0319b6e16385163f66b647091a48550"
             ],
             [
-                "test_umi-consensus.bam:md5,122d60b069b76a217b6fe91d1765dae3"
+                "test_umi-consensus.bam:md5,d77fca0cc9d628d9966556d64be72460"
             ],
             [
-                "test.sorted.cram:md5,450f711661b0222e8496ea3140fe7fff"
+                "test.sorted.cram:md5,d07bc9529dff41207c4cac3ecbd47071"
             ],
             "No VCF files",
             [
diff --git a/tests/save_mapped.nf.test.snap b/tests/save_mapped.nf.test.snap
index 1c277f5804..36e862f55e 100644
--- a/tests/save_mapped.nf.test.snap
+++ b/tests/save_mapped.nf.test.snap
@@ -7,11 +7,11 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 52e6dde2b8..6ecc236aa4 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -7,11 +7,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
                     "cnvkit": "0.9.11"
@@ -377,11 +377,11 @@
             9,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/sentieon.nf.test.snap b/tests/sentieon.nf.test.snap
index 5d52871cb0..5387d5cdb8 100644
--- a/tests/sentieon.nf.test.snap
+++ b/tests/sentieon.nf.test.snap
@@ -4,7 +4,7 @@
             18,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/sentieon_aligner_bwamem.nf.test.snap b/tests/sentieon_aligner_bwamem.nf.test.snap
index 4e05c5ff0c..48c7ef8233 100644
--- a/tests/sentieon_aligner_bwamem.nf.test.snap
+++ b/tests/sentieon_aligner_bwamem.nf.test.snap
@@ -7,7 +7,7 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -90,16 +90,16 @@
             9,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "1.1.0"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_INTERVALLISTTOBED": {
                     "gatk4": "4.6.2.0"
@@ -145,7 +145,7 @@
                 "reports/sentieon_dedup/test/test.dedup.cram.metrics.multiqc.tsv"
             ],
             [
-                "test-test_L1.fastp.json:md5,d9107ec414e44408d0698b167cc445f9"
+                "test-test_L1.fastp.json:md5,5146ada16c3aae0e55e3e993b9d587af"
             ],
             "No BAM files",
             [
@@ -167,7 +167,7 @@
             5,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/sentieon_dedup.nf.test.snap b/tests/sentieon_dedup.nf.test.snap
index 4f48082aaf..b2424d75a7 100644
--- a/tests/sentieon_dedup.nf.test.snap
+++ b/tests/sentieon_dedup.nf.test.snap
@@ -583,7 +583,7 @@
                     "gawk": "5.3.0"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
@@ -778,7 +778,7 @@
                     "gawk": "5.3.0"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/spark.nf.test.snap b/tests/spark.nf.test.snap
index 9606f3e91b..294289be2b 100644
--- a/tests/spark.nf.test.snap
+++ b/tests/spark.nf.test.snap
@@ -4,11 +4,11 @@
             18,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -253,11 +253,11 @@
             11,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 89c1068628..17b602b3c5 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -7,11 +7,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/umi_fastp.nf.test.snap b/tests/umi_fastp.nf.test.snap
index 54e35ad2af..ce8a858825 100644
--- a/tests/umi_fastp.nf.test.snap
+++ b/tests/umi_fastp.nf.test.snap
@@ -4,23 +4,23 @@
             18,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "FASTP": {
-                    "fastp": "0.24.0"
+                    "fastp": "1.1.0"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
@@ -318,7 +318,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,5e049e05573f1afd0bb893f2cb4076c6",
                 "mosdepth_perchrom.txt:md5,45bca136491a7675ebba87def2534f26",
                 "multiqc_citations.txt:md5,0e2971e7a873c92592112775fa99fb02",
-                "multiqc_fastp.txt:md5,8e11d751e997877e4ffc487c52756f62",
+                "multiqc_fastp.txt:md5,542e41a81f66bd647eff0e741a377b04",
                 "multiqc_fastqc.txt:md5,0ca2cba4204d9076a1eb17596379d10c",
                 "picard_MarkIlluminaAdapters_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -327,7 +327,7 @@
                 "samtools-stats-dp.txt:md5,a6f11f838a72299e4913a54dd327f2e6",
                 "samtools_alignment_plot.txt:md5,111d8b457e8c34dc919a527f12f27d4a",
                 "samtools_insert_size.txt:md5,5dbef29f8a260b9aa05de422676ab81a",
-                "test-test_L1.fastp.json:md5,d9107ec414e44408d0698b167cc445f9",
+                "test-test_L1.fastp.json:md5,5146ada16c3aae0e55e3e993b9d587af",
                 "test.md.mosdepth.global.dist.txt:md5,40bf266b2080717f92b405cb42fab4a7",
                 "test.md.mosdepth.region.dist.txt:md5,94b080042f51f484fa178339cc9324bc",
                 "test.md.mosdepth.summary.txt:md5,7958c5422bb4b5181afe9782e38a735a",
diff --git a/tests/umi_fgbio.nf.test.snap b/tests/umi_fgbio.nf.test.snap
index 2504ee455e..da94f581b4 100644
--- a/tests/umi_fgbio.nf.test.snap
+++ b/tests/umi_fgbio.nf.test.snap
@@ -27,17 +27,17 @@
                     "samtools": 1.21
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CALLUMICONSENSUS": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "CAT_FASTQ": {
-                    "cat": 9.5
+                    "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
                     "samtools": 1.21
@@ -52,7 +52,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FASTQTOBAM": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
@@ -68,7 +68,7 @@
                     "samtools": "1.21"
                 },
                 "GROUPREADSBYUMI": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "INDEX_CRAM": {
                     "samtools": 1.21
@@ -276,7 +276,8 @@
                 "reports/samtools/test/test.md.cram.stats",
                 "reports/samtools/test/test.recal.cram.stats",
                 "reports/umi",
-                "reports/umi/test_umi-grouped_histogram.txt"
+                "reports/umi/test_umi-grouped_histogram.txt",
+                "reports/umi/test_umi-grouped_read-metrics.txt"
             ],
             [
                 "fastqc-status-check-heatmap.txt:md5,92fcedba625d8394492d1ef6e2eb64f0",
@@ -290,36 +291,37 @@
                 "fastqc_sequence_counts_plot.txt:md5,1a4aab87f1bd4fd9f69cabef40f5e5ed",
                 "fastqc_sequence_duplication_levels_plot.txt:md5,72e6366160d000a2990b2bbd321300a7",
                 "fastqc_sequence_length_distribution_plot.txt:md5,7c07faf0c0b90613cdc82a9f09bc1f19",
-                "mosdepth-coverage-per-contig-single.txt:md5,800ad271050c46d24bf53c0f8bdf7b6e",
-                "mosdepth-cumcoverage-dist-id.txt:md5,3c526ec6da433233b419bdbdd19a1b56",
-                "mosdepth_perchrom.txt:md5,800ad271050c46d24bf53c0f8bdf7b6e",
+                "mosdepth-coverage-per-contig-single.txt:md5,fc8d90478e230b3cbad16ef68ede3a62",
+                "mosdepth-cumcoverage-dist-id.txt:md5,7985d3196b234be9907f6c417c4fe0df",
+                "mosdepth_perchrom.txt:md5,fc8d90478e230b3cbad16ef68ede3a62",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
                 "multiqc_fastqc.txt:md5,0ca2cba4204d9076a1eb17596379d10c",
                 "picard_MarkIlluminaAdapters_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,4d27768d75a69a61daf23bf5efe17687",
-                "samtools_alignment_plot.txt:md5,3c2a882852a7ffe06610c9027f0a312a",
-                "samtools_insert_size.txt:md5,728c8cd8979768e4912f608f59fe1222",
-                "test.md.mosdepth.global.dist.txt:md5,09d22913aa50a0207f97a3f85b182c6e",
-                "test.md.mosdepth.region.dist.txt:md5,61676a4a3668c0e84eb0f56dc6bda1ae",
-                "test.md.mosdepth.summary.txt:md5,9bbea5e4d213a51f501c2aadff8d4526",
-                "test.md.regions.bed.gz:md5,e24c18ad56e54376c41fdaacec372f9e",
-                "test.md.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537",
-                "test.recal.mosdepth.global.dist.txt:md5,09d22913aa50a0207f97a3f85b182c6e",
-                "test.recal.mosdepth.region.dist.txt:md5,61676a4a3668c0e84eb0f56dc6bda1ae",
-                "test.recal.mosdepth.summary.txt:md5,9bbea5e4d213a51f501c2aadff8d4526",
-                "test.recal.regions.bed.gz:md5,e24c18ad56e54376c41fdaacec372f9e",
-                "test.recal.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537",
-                "test_umi-grouped_histogram.txt:md5,85292e9acb83edf17110dce17be27f44"
+                "samtools-stats-dp.txt:md5,49a0910af4cea91b46a1df076c08c5b5",
+                "samtools_alignment_plot.txt:md5,60b8f1d48124a8f28b06dc0d15ca4d59",
+                "samtools_insert_size.txt:md5,ef2a81138667814cda3e5c8c2628c549",
+                "test.md.mosdepth.global.dist.txt:md5,bb51e76c9cf2f3929cb5ce560f559dd6",
+                "test.md.mosdepth.region.dist.txt:md5,11dc4683f9038de1938b4874e02974d6",
+                "test.md.mosdepth.summary.txt:md5,d6cda5af0f2bf21973e256cf198aa47a",
+                "test.md.regions.bed.gz:md5,b420210d41279bbc93f211491f7d4f02",
+                "test.md.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.mosdepth.global.dist.txt:md5,bb51e76c9cf2f3929cb5ce560f559dd6",
+                "test.recal.mosdepth.region.dist.txt:md5,11dc4683f9038de1938b4874e02974d6",
+                "test.recal.mosdepth.summary.txt:md5,d6cda5af0f2bf21973e256cf198aa47a",
+                "test.recal.regions.bed.gz:md5,b420210d41279bbc93f211491f7d4f02",
+                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test_umi-grouped_histogram.txt:md5,85292e9acb83edf17110dce17be27f44",
+                "test_umi-grouped_read-metrics.txt:md5,cb2aecfe82357aa06e091d69b4606b9e"
             ],
             [
-                "test_umi-consensus.bam:md5,18d420720f72366289d3a324f4552522"
+                "test_umi-consensus.bam:md5,86ddd035aced3a9e7b6e618794824050"
             ],
             [
-                "test.md.cram:md5,2d0c176fed158d84a061b69e1947994c",
-                "test.recal.cram:md5,6927569ca52dbba512d27f8742bc6aae"
+                "test.md.cram:md5,a8f60b321cc24e782dcf6470fd310b08",
+                "test.recal.cram:md5,a8f60b321cc24e782dcf6470fd310b08"
             ],
             "No VCF files",
             [
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index a61ced6604..3c42b848d9 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -10,7 +10,7 @@
                     "gawk": "5.3.0"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
@@ -269,14 +269,14 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CAT_FASTQ": {
-                    "cat": 9.5
+                    "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
                     "samtools": 1.21
@@ -291,7 +291,7 @@
                     "fastqc": "0.12.1"
                 },
                 "FGBIO_COPYUMIFROMREADNAME": {
-                    "fgbio": "2.4.0"
+                    "fgbio": "3.1.2"
                 },
                 "GATK4_APPLYBQSR": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index 33a2044c8f..4415537702 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -13,11 +13,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
                     "cnvkit": "0.9.11"
@@ -654,11 +654,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
                     "cnvkit": "0.9.11"
@@ -1148,11 +1148,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CALCULATECONTAMINATION": {
                     "gatk4": "4.6.2.0"
diff --git a/tests/variant_calling_controlfreec.nf.test.snap b/tests/variant_calling_controlfreec.nf.test.snap
index 812a819430..2e78020160 100644
--- a/tests/variant_calling_controlfreec.nf.test.snap
+++ b/tests/variant_calling_controlfreec.nf.test.snap
@@ -32,7 +32,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_CHR_DIR": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
@@ -189,7 +189,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_CHR_DIR": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
@@ -361,7 +361,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_CHR_DIR": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
@@ -480,7 +480,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_CHR_DIR": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 683a3576c9..94c0efd52b 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -221,11 +221,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -626,11 +626,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -995,11 +995,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "FASTQC": {
                     "fastqc": "0.12.1"
@@ -1547,11 +1547,11 @@
                     "bcftools": "1.23.1"
                 },
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "BWAMEM1_MEM": {
-                    "bwa": "0.7.18-r1243-dirty",
-                    "samtools": 1.21
+                    "bwa": "0.7.19-r1273",
+                    "samtools": "1.22.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/variant_calling_msisensor2.nf.test.snap b/tests/variant_calling_msisensor2.nf.test.snap
index 19d0e935d5..23997e71d4 100644
--- a/tests/variant_calling_msisensor2.nf.test.snap
+++ b/tests/variant_calling_msisensor2.nf.test.snap
@@ -11,7 +11,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
@@ -104,7 +104,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
@@ -184,7 +184,7 @@
                     "tabix": "1.21"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
-                    "untar": 1.34
+                    "untar": "1.34"
                 }
             },
             [
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index 57a9c11a95..a6adc2992f 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -606,7 +606,7 @@
                     "bcftools": "1.23.1"
                 },
                 "BUILD_INTERVALS": {
-                    "gawk": "5.3.0"
+                    "gawk": "5.3.1"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/tests/variant_calling_sentieon_tnscope.nf.test.snap b/tests/variant_calling_sentieon_tnscope.nf.test.snap
index 7278a9d721..ee0421f02f 100644
--- a/tests/variant_calling_sentieon_tnscope.nf.test.snap
+++ b/tests/variant_calling_sentieon_tnscope.nf.test.snap
@@ -4,7 +4,7 @@
             11,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -91,7 +91,7 @@
             19,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -204,7 +204,7 @@
             10,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
@@ -292,7 +292,7 @@
             13,
             {
                 "BWAMEM1_INDEX": {
-                    "bwa": "0.7.18-r1243-dirty"
+                    "bwa": "0.7.19-r1273"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 8bb7129737..2ee53dc1be 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -168,9 +168,6 @@ workflow SAREK {
             true,
         )
 
-        versions = versions.mix(SPRING_DECOMPRESS_TO_R1_FQ.out.versions)
-        versions = versions.mix(SPRING_DECOMPRESS_TO_R2_FQ.out.versions)
-        versions = versions.mix(SPRING_DECOMPRESS_TO_FQ_PAIR.out.versions)
 
         two_fastq_gz_from_spring = r1_fastq_gz_from_spring.fastq.join(r2_fastq_gz_from_spring.fastq).map { meta, fastq_1, fastq_2 -> [meta, [fastq_1, fastq_2]] }
 

From c8c2348f8adae8049f7a7156a7eb00174ff1b52c Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Thu, 23 Jul 2026 11:35:49 +0200
Subject: [PATCH 16/27] chore(modules): migrate variant-calling modules to
 versions topic channel (#2240)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates variant-calling / VCF modules to the `versions` topic channel.
**Stacked on #2239** (base: `topic/alignment-utils`).

### Changes
- Updates `freebayes`, `strelka`, `manta`, `tiddit`, `lofreq`, `svdb`,
`vcflib`, `vcftools` to their topic-channel versions.
- Removes the corresponding `.out.versions` wiring from subworkflows
(empty `versions` channels left in place for now — a follow-up PR will
strip now-unused init/emit pipeline-wide).
- Tool bumps: svdb 2.8.2 → 2.8.4, tiddit 3.6.1 → 3.9.5, vcftools 0.1.16
→ 0.1.17.

Test snapshots need regenerating in CI.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   4 +
 modules.json                                  |  22 +-
 modules/nf-core/freebayes/main.nf             |  19 +-
 modules/nf-core/freebayes/meta.yml            |  77 ++-
 modules/nf-core/lofreq/callparallel/main.nf   |  43 +-
 modules/nf-core/lofreq/callparallel/meta.yml  |  41 +-
 .../nf-core/manta/germline/environment.yml    |   2 +-
 modules/nf-core/manta/germline/main.nf        |  28 +-
 modules/nf-core/manta/germline/meta.yml       |  28 +-
 modules/nf-core/manta/somatic/environment.yml |   2 +-
 modules/nf-core/manta/somatic/main.nf         |  14 +-
 modules/nf-core/manta/somatic/meta.yml        |  28 +-
 .../nf-core/manta/tumoronly/environment.yml   |   2 +-
 modules/nf-core/manta/tumoronly/main.nf       |  14 +-
 modules/nf-core/manta/tumoronly/meta.yml      |  28 +-
 .../nf-core/strelka/germline/environment.yml  |   2 +-
 modules/nf-core/strelka/germline/main.nf      |  16 +-
 modules/nf-core/strelka/germline/meta.yml     |  42 +-
 .../nf-core/strelka/somatic/environment.yml   |   2 +-
 modules/nf-core/strelka/somatic/main.nf       |  16 +-
 modules/nf-core/strelka/somatic/meta.yml      |  44 +-
 modules/nf-core/svdb/merge/environment.yml    |   5 +-
 modules/nf-core/svdb/merge/main.nf            |  53 +-
 modules/nf-core/svdb/merge/meta.yml           |  85 ++-
 modules/nf-core/tiddit/sv/environment.yml     |   2 +-
 modules/nf-core/tiddit/sv/main.nf             |  42 +-
 modules/nf-core/tiddit/sv/meta.yml            |  52 +-
 modules/nf-core/vcflib/vcffilter/main.nf      |  21 +-
 modules/nf-core/vcflib/vcffilter/meta.yml     |  29 +-
 modules/nf-core/vcftools/environment.yml      |   2 +-
 modules/nf-core/vcftools/main.nf              |  34 +-
 modules/nf-core/vcftools/meta.yml             | 483 +++++++++++-------
 .../bam_variant_calling_freebayes/main.nf     |   2 -
 .../bam_variant_calling_germline_all/main.nf  |   4 +-
 .../main.nf                                   |   4 -
 .../main.nf                                   |   4 -
 .../bam_variant_calling_single_tiddit/main.nf |   7 +-
 .../bam_variant_calling_somatic_all/main.nf   |   4 +-
 .../bam_variant_calling_somatic_manta/main.nf |   4 -
 .../main.nf                                   |   4 -
 .../main.nf                                   |  12 +-
 .../main.nf                                   |   4 +-
 .../main.nf                                   |   4 -
 .../main.nf                                   |   4 -
 .../local/vcf_qc_bcftools_vcftools/main.nf    |   5 -
 tests/aligner-parabricks.nf.test.snap         |   8 +-
 tests/bbsplit.nf.test.snap                    |  16 +-
 tests/default.nf.test.snap                    |  16 +-
 ...joint_calling_haplotypecaller.nf.test.snap |  16 +-
 tests/joint_calling_mutect2.nf.test.snap      |  16 +-
 tests/postprocess_concatenation.nf.test.snap  |   8 +-
 ...s_concatenation_normalization.nf.test.snap |  16 +-
 tests/postprocess_consensus.nf.test.snap      |  24 +-
 tests/postprocess_filtering.nf.test.snap      |   8 +-
 tests/postprocess_normalization.nf.test.snap  |   8 +-
 tests/postprocess_varlociraptor.nf.test.snap  |  24 +-
 tests/save_output_as_bam.nf.test.snap         |   8 +-
 ...art_from_preparerecalibration.nf.test.snap |  16 +-
 tests/start_from_recalibration.nf.test.snap   |  16 +-
 tests/tumor-normal-pair.nf.test.snap          |   8 +-
 tests/umi_in_read_names.nf.test.snap          |  16 +-
 tests/variant_calling_all.nf.test.snap        |  54 +-
 .../variant_calling_deepvariant.nf.test.snap  |  32 +-
 tests/variant_calling_freebayes.nf.test.snap  |  48 +-
 ...riant_calling_haplotypecaller.nf.test.snap |  32 +-
 tests/variant_calling_lofreq.nf.test.snap     |  12 +
 tests/variant_calling_manta.nf.test.snap      |  56 +-
 tests/variant_calling_mpileup.nf.test.snap    |  32 +-
 tests/variant_calling_muse.nf.test.snap       |  16 +-
 tests/variant_calling_mutect2.nf.test.snap    |  32 +-
 ...ant_calling_sentieon_dnascope.nf.test.snap |  24 +-
 ...ling_sentieon_haplotypecaller.nf.test.snap |  32 +-
 ...iant_calling_sentieon_tnscope.nf.test.snap |  32 +-
 tests/variant_calling_strelka.nf.test.snap    |  40 +-
 tests/variant_calling_strelka_bp.nf.test.snap |  16 +-
 tests/variant_calling_tiddit.nf.test.snap     |  34 +-
 workflows/sarek.nf                            |   1 -
 77 files changed, 1365 insertions(+), 696 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 12fcf16783..3e28d64d3f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,6 +20,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2235](https://github.com/nf-core/sarek/pull/2235) - germline CNVKIT reuses the shared `CRAM_TO_BAM` conversion instead of re-converting CRAM internally, avoiding a duplicate conversion. Side effect: with `--step variant_calling` (user-supplied CRAM/BAM), CNVKit output files are named after the input file rather than the sample; runs from FASTQ are unaffected.
 - [#2238](https://github.com/nf-core/sarek/pull/2238) - Migrate `gatk4`/`gatk4spark` modules to the versions topic channel (bumps gatk4spark 4.6.1.0 → 4.6.2.0)
 - [#2239](https://github.com/nf-core/sarek/pull/2239) - Migrate alignment/UMI/utility modules (`bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`, `gunzip`, `untar`, `unzip`, `spring`) to the versions topic channel (fastp 0.24.0 → 1.1.0)
+- [#2240](https://github.com/nf-core/sarek/pull/2240) - Migrate variant-calling modules (`freebayes`, `strelka`, `manta`, `tiddit`, `lofreq`, `svdb`, `vcflib`, `vcftools`) to the versions topic channel
 
 ### Fixed
 
@@ -44,6 +45,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | fgbio         | 2.4.0       | 3.1.2       |
 | gawk          | 5.3.0       | 5.3.1       |
 | pigz          | 2.3.4       | 2.8         |
+| svdb          | 2.8.2       | 2.8.4       |
+| tiddit        | 3.6.1       | 3.9.5       |
+| vcftools      | 0.1.16      | 0.1.17      |
 
 ### Dependencies - plugins
 
diff --git a/modules.json b/modules.json
index d7a3851c68..e883cb77a7 100644
--- a/modules.json
+++ b/modules.json
@@ -203,7 +203,7 @@
                     },
                     "freebayes": {
                         "branch": "master",
-                        "git_sha": "d04951ee68e3e8b875ebf5ddb7ba6e05233624c1",
+                        "git_sha": "6fd0d62609c3a24b08c9df3df45857f974efc7ad",
                         "installed_by": ["modules"]
                     },
                     "gatk4/applybqsr": {
@@ -348,22 +348,22 @@
                     },
                     "lofreq/callparallel": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9707facbacc9c5c02ecf0b192f0604a29351b00a",
                         "installed_by": ["modules"]
                     },
                     "manta/germline": {
                         "branch": "master",
-                        "git_sha": "ae6b18e8930fe66595c8b08633b484d42970a564",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "manta/somatic": {
                         "branch": "master",
-                        "git_sha": "ae6b18e8930fe66595c8b08633b484d42970a564",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "manta/tumoronly": {
                         "branch": "master",
-                        "git_sha": "ae6b18e8930fe66595c8b08633b484d42970a564",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "mosdepth": {
@@ -533,17 +533,17 @@
                     },
                     "strelka/germline": {
                         "branch": "master",
-                        "git_sha": "039730fab3f0150585ad46c402c6bf95396d88b5",
+                        "git_sha": "db2491458130c466967c4e9b44686314c3f37141",
                         "installed_by": ["modules"]
                     },
                     "strelka/somatic": {
                         "branch": "master",
-                        "git_sha": "039730fab3f0150585ad46c402c6bf95396d88b5",
+                        "git_sha": "db2491458130c466967c4e9b44686314c3f37141",
                         "installed_by": ["modules"]
                     },
                     "svdb/merge": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6bc8ff03ace2cb373f683b9502ce79930c8a07f0",
                         "installed_by": ["modules"]
                     },
                     "tabix/bgziptabix": {
@@ -558,7 +558,7 @@
                     },
                     "tiddit/sv": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "untar": {
@@ -593,12 +593,12 @@
                     },
                     "vcflib/vcffilter": {
                         "branch": "master",
-                        "git_sha": "401ec2b2b8d0938d12ae4f9e25819e14596b8f83",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "vcftools": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "094186cc8b196ebdded57d596d741611ff571cf3",
                         "installed_by": ["modules"]
                     },
                     "yte": {
diff --git a/modules/nf-core/freebayes/main.nf b/modules/nf-core/freebayes/main.nf
index 9d949add0b..4f3f8bb35d 100644
--- a/modules/nf-core/freebayes/main.nf
+++ b/modules/nf-core/freebayes/main.nf
@@ -3,9 +3,9 @@ process FREEBAYES {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/freebayes:1.3.10--hbefcdb2_0'
-        : 'biocontainers/freebayes:1.3.10--hbefcdb2_0'}"
+        : 'quay.io/biocontainers/freebayes:1.3.10--hbefcdb2_0'}"
 
     input:
     tuple val(meta), path(input_1), path(input_1_index), path(input_2), path(input_2_index), path(target_bed)
@@ -17,7 +17,8 @@ process FREEBAYES {
 
     output:
     tuple val(meta), path("*.vcf.gz"), emit: vcf
-    path "versions.yml"              , emit: versions
+    tuple val("${task.process}"), val('freebayes'), eval('freebayes --version 2>&1 | sed "s/version:\s*v//g"'), emit: versions_freebayes, topic: versions
+
 
     when:
     task.ext.when == null || task.ext.when
@@ -41,21 +42,11 @@ process FREEBAYES {
         ${input} > ${prefix}.vcf
 
     bgzip ${prefix}.vcf
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        freebayes: \$(echo \$(freebayes --version 2>&1) | sed 's/version:\s*v//g' )
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
-    echo | gzip > ${prefix}.vcf.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        freebayes: \$(echo \$(freebayes --version 2>&1) | sed 's/version:\s*v//g' )
-    END_VERSIONS
+    echo "" | gzip > ${prefix}.vcf.gz
     """
 }
diff --git a/modules/nf-core/freebayes/meta.yml b/modules/nf-core/freebayes/meta.yml
index 5593bcaaa9..17cc5d172b 100644
--- a/modules/nf-core/freebayes/meta.yml
+++ b/modules/nf-core/freebayes/meta.yml
@@ -15,7 +15,8 @@ tools:
       documentation: https://github.com/freebayes/freebayes
       tool_dev_url: https://github.com/freebayes/freebayes
       doi: "10.48550/arXiv.1207.3907"
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:freebayes
 input:
   - - meta:
@@ -27,28 +28,37 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+          - edam: http://edamontology.org/format_3462 # CRAM
+          - edam: http://edamontology.org/format_2573 # SAM
     - input_1_index:
         type: file
         description: BAM/CRAM/SAM index file
         pattern: "*.{bai,crai}"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # BAI/CRAI index
     - input_2:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+          - edam: http://edamontology.org/format_3462 # CRAM
+          - edam: http://edamontology.org/format_2573 # SAM
     - input_2_index:
         type: file
         description: BAM/CRAM/SAM index file
         pattern: "*.{bai,crai}"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # BAI/CRAI index
     - target_bed:
         type: file
-        description: Optional - Limit analysis to targets listed in this BED-format
-          FILE.
+        description: Optional - Limit analysis to targets listed in this
+          BED-format FILE.
         pattern: "*.bed"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_3003 # BED
   - - meta2:
         type: map
         description: |
@@ -58,7 +68,8 @@ input:
         type: file
         description: reference fasta file
         pattern: ".{fa,fa.gz,fasta,fasta.gz}"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_1929 # FASTA
   - - meta3:
         type: map
         description: |
@@ -68,7 +79,8 @@ input:
         type: file
         description: reference fasta file index
         pattern: "*.{fa,fasta}.fai"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # FASTA index
   - - meta4:
         type: map
         description: |
@@ -76,10 +88,11 @@ input:
           e.g. [ id:'test_samples' ]
     - samples:
         type: file
-        description: Optional - Limit analysis to samples listed (one per line) in the
-          FILE.
+        description: Optional - Limit analysis to samples listed (one per line) in
+          the FILE.
         pattern: "*.txt"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_2330 # Textual format
   - - meta5:
         type: map
         description: |
@@ -87,10 +100,11 @@ input:
           e.g. [ id:'test_populations' ]
     - populations:
         type: file
-        description: Optional - Each line of FILE should list a sample and a population
-          which it is part of.
+        description: Optional - Each line of FILE should list a sample and a
+          population which it is part of.
         pattern: "*.txt"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_2330 # Textual format
   - - meta6:
         type: map
         description: |
@@ -104,7 +118,8 @@ input:
           or a region-specific format:
           seq_name start end sample_name copy_number
         pattern: "*.bed"
-        ontologies: []
+        ontologies:
+          - edam: http://edamontology.org/format_3003 # BED
 output:
   vcf:
     - - meta:
@@ -117,14 +132,28 @@ output:
           description: Compressed VCF file
           pattern: "*.vcf.gz"
           ontologies:
-            - edam: http://edamontology.org/format_3989 # GZIP format
+            - edam: http://edamontology.org/format_3989
+  versions_freebayes:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - freebayes:
+          type: string
+          description: The name of the tool
+      - freebayes --version 2>&1 | sed "s/version:\s*v//g":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software version
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - freebayes:
+          type: string
+          description: The name of the tool
+      - freebayes --version 2>&1 | sed "s/version:\s*v//g":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@maxibor"
   - "@FriederikeHanssen"
diff --git a/modules/nf-core/lofreq/callparallel/main.nf b/modules/nf-core/lofreq/callparallel/main.nf
index 93f9a3dfb1..0b187150db 100644
--- a/modules/nf-core/lofreq/callparallel/main.nf
+++ b/modules/nf-core/lofreq/callparallel/main.nf
@@ -1,21 +1,21 @@
 process LOFREQ_CALLPARALLEL {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_high'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/lofreq:2.1.5--py38h588ecb2_4' :
-        'biocontainers/lofreq:2.1.5--py38h588ecb2_4' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/lofreq:2.1.5--py38h588ecb2_4'
+        : 'quay.io/biocontainers/lofreq:2.1.5--py38h588ecb2_4'}"
 
     input:
-    tuple val(meta) , path(bam), path(bai), path(intervals)
+    tuple val(meta), path(bam), path(bai), path(intervals)
     tuple val(meta2), path(fasta)
     tuple val(meta3), path(fai)
 
     output:
-    tuple val(meta), path("*.vcf.gz")    , emit: vcf
+    tuple val(meta), path("*.vcf.gz"),     emit: vcf
     tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi
-    path "versions.yml"                  , emit: versions
+    tuple val("${task.process}"), val('lofreq'), eval("lofreq version | sed -n '1s/^.* //p'"), emit: versions_lofreq, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -25,35 +25,29 @@ process LOFREQ_CALLPARALLEL {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def options_intervals = intervals ? "-l ${intervals}" : ""
 
-    def alignment_cram =  bam.Extension == "cram" ? true : false
-    def alignment_bam = bam.Extension == "bam" ? true : false
+    def alignment_cram = bam.Extension == "cram" ? true : false
     def alignment_out = alignment_cram ? bam.BaseName + ".bam" : "${bam}"
 
     def samtools_cram_convert = ''
-    samtools_cram_convert += alignment_cram ? "    samtools view -T ${fasta} ${bam} -@ $task.cpus -o ${alignment_out}\n" : ''
+    samtools_cram_convert += alignment_cram ? "    samtools view -T ${fasta} ${bam} -@ ${task.cpus} -o ${alignment_out}\n" : ''
     samtools_cram_convert += alignment_cram ? "    samtools index ${alignment_out}\n" : ''
 
     def samtools_cram_remove = ''
     samtools_cram_remove += alignment_cram ? "    rm ${alignment_out}\n" : ''
     samtools_cram_remove += alignment_cram ? "    rm ${alignment_out}.bai\n " : ''
     """
-    $samtools_cram_convert
+    ${samtools_cram_convert}
 
     lofreq \\
         call-parallel \\
-        --pp-threads $task.cpus \\
-        $args \\
-        $options_intervals \\
-        -f $fasta \\
+        --pp-threads ${task.cpus} \\
+        ${args} \\
+        ${options_intervals} \\
+        -f ${fasta} \\
         -o ${prefix}.vcf.gz \\
-        $alignment_out
+        ${alignment_out}
 
-    $samtools_cram_remove
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        lofreq: \$(echo \$(lofreq version 2>&1) | sed 's/^version: //; s/ *commit.*\$//')
-    END_VERSIONS
+    ${samtools_cram_remove}
     """
 
     stub:
@@ -61,10 +55,5 @@ process LOFREQ_CALLPARALLEL {
     """
     echo "" | gzip > ${prefix}.vcf.gz
     echo "" | gzip > ${prefix}.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        lofreq: \$(echo \$(lofreq version 2>&1) | sed 's/^version: //; s/ *commit.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/lofreq/callparallel/meta.yml b/modules/nf-core/lofreq/callparallel/meta.yml
index 25a33e85c4..7e74398d40 100644
--- a/modules/nf-core/lofreq/callparallel/meta.yml
+++ b/modules/nf-core/lofreq/callparallel/meta.yml
@@ -25,14 +25,17 @@ input:
         type: file
         description: Tumor sample sorted BAM file
         pattern: "*.{bam}"
+        ontologies: []
     - bai:
         type: file
         description: BAM index file
         pattern: "*.{bam.bai}"
+        ontologies: []
     - intervals:
         type: file
         description: BED file containing target regions for variant calling
         pattern: "*.{bed}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -42,6 +45,7 @@ input:
         type: file
         description: Reference genome FASTA file
         pattern: "*.{fasta}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -51,9 +55,10 @@ input:
         type: file
         description: Reference genome FASTA index file
         pattern: "*.{fai}"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -62,8 +67,9 @@ output:
           type: file
           description: Predicted variants file
           pattern: "*.{vcf}"
-  - tbi:
-      - meta:
+          ontologies: []
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -72,11 +78,28 @@ output:
           type: file
           description: Index of vcf file
           pattern: "*.{vcf.gz.tbi}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_lofreq:
+    - - ${task.process}:
+          type: string
+          description: Process which generated the version
+      - lofreq:
+          type: string
+          description: Tool name
+      - "lofreq version | sed -n '1s/^.* //p'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: Process which generated the version
+      - lofreq:
+          type: string
+          description: Tool name
+      - "lofreq version | sed -n '1s/^.* //p'":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@kaurravneet4123"
   - "@bjohnnyd"
diff --git a/modules/nf-core/manta/germline/environment.yml b/modules/nf-core/manta/germline/environment.yml
index 3804c07f42..697c36d1c8 100644
--- a/modules/nf-core/manta/germline/environment.yml
+++ b/modules/nf-core/manta/germline/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::python=2.7.15
   - bioconda::manta=1.6.0
+  - conda-forge::python=2.7.15
diff --git a/modules/nf-core/manta/germline/main.nf b/modules/nf-core/manta/germline/main.nf
index 0fad64f31e..ddb19689b7 100644
--- a/modules/nf-core/manta/germline/main.nf
+++ b/modules/nf-core/manta/germline/main.nf
@@ -4,7 +4,7 @@ process MANTA_GERMLINE {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f6/f696c93e6209e33ac0d15f1ecfa799bc67329eec07b0569e065ea8b220b53953/data' :
         'community.wave.seqera.io/library/manta_python:0eb71149179b3920' }"
 
@@ -16,13 +16,13 @@ process MANTA_GERMLINE {
     path(config)
 
     output:
-    tuple val(meta), path("*candidate_small_indels.vcf.gz")    , emit: candidate_small_indels_vcf
-    tuple val(meta), path("*candidate_small_indels.vcf.gz.tbi"), emit: candidate_small_indels_vcf_tbi
-    tuple val(meta), path("*candidate_sv.vcf.gz")              , emit: candidate_sv_vcf
-    tuple val(meta), path("*candidate_sv.vcf.gz.tbi")          , emit: candidate_sv_vcf_tbi
-    tuple val(meta), path("*diploid_sv.vcf.gz")                , emit: diploid_sv_vcf
-    tuple val(meta), path("*diploid_sv.vcf.gz.tbi")            , emit: diploid_sv_vcf_tbi
-    path "versions.yml"                                        , emit: versions
+    tuple val(meta), path("*candidate_small_indels.vcf.gz")                     , emit: candidate_small_indels_vcf
+    tuple val(meta), path("*candidate_small_indels.vcf.gz.tbi")                 , emit: candidate_small_indels_vcf_tbi
+    tuple val(meta), path("*candidate_sv.vcf.gz")                               , emit: candidate_sv_vcf
+    tuple val(meta), path("*candidate_sv.vcf.gz.tbi")                           , emit: candidate_sv_vcf_tbi
+    tuple val(meta), path("*diploid_sv.vcf.gz")                                 , emit: diploid_sv_vcf
+    tuple val(meta), path("*diploid_sv.vcf.gz.tbi")                             , emit: diploid_sv_vcf_tbi
+    tuple val("${task.process}"), val("manta"), eval("configManta.py --version"), topic: versions, emit: versions_manta
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,7 +30,7 @@ process MANTA_GERMLINE {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def input_files = input.collect{"--bam ${it}"}.join(' ')
+    def input_files = input.collect{ bam -> "--bam ${bam}"}.join(' ')
     def options_manta = target_bed ? "--callRegions $target_bed" : ""
     def config_option = config ? "--config ${config}" : ""
     """
@@ -56,11 +56,6 @@ process MANTA_GERMLINE {
         ${prefix}.diploid_sv.vcf.gz
     mv manta/results/variants/diploidSV.vcf.gz.tbi \\
         ${prefix}.diploid_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 
     stub:
@@ -72,10 +67,5 @@ process MANTA_GERMLINE {
     touch ${prefix}.candidate_sv.vcf.gz.tbi
     echo "" | gzip > ${prefix}.diploid_sv.vcf.gz
     touch ${prefix}.diploid_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/manta/germline/meta.yml b/modules/nf-core/manta/germline/meta.yml
index 9774e1a059..83ff420b88 100644
--- a/modules/nf-core/manta/germline/meta.yml
+++ b/modules/nf-core/manta/germline/meta.yml
@@ -137,13 +137,29 @@ output:
           description: Index for gzipped VCF file containing variants
           pattern: "*.{vcf.gz.tbi}"
           ontologies: []
+  versions_manta:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@maxulysse"
   - "@ramprasadn"
diff --git a/modules/nf-core/manta/somatic/environment.yml b/modules/nf-core/manta/somatic/environment.yml
index 3804c07f42..697c36d1c8 100644
--- a/modules/nf-core/manta/somatic/environment.yml
+++ b/modules/nf-core/manta/somatic/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::python=2.7.15
   - bioconda::manta=1.6.0
+  - conda-forge::python=2.7.15
diff --git a/modules/nf-core/manta/somatic/main.nf b/modules/nf-core/manta/somatic/main.nf
index 317e3f484e..f2bb4d2f38 100644
--- a/modules/nf-core/manta/somatic/main.nf
+++ b/modules/nf-core/manta/somatic/main.nf
@@ -4,7 +4,7 @@ process MANTA_SOMATIC {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f6/f696c93e6209e33ac0d15f1ecfa799bc67329eec07b0569e065ea8b220b53953/data' :
         'community.wave.seqera.io/library/manta_python:0eb71149179b3920' }"
 
@@ -23,7 +23,7 @@ process MANTA_SOMATIC {
     tuple val(meta), path("*.diploid_sv.vcf.gz.tbi")             , emit: diploid_sv_vcf_tbi
     tuple val(meta), path("*.somatic_sv.vcf.gz")                 , emit: somatic_sv_vcf
     tuple val(meta), path("*.somatic_sv.vcf.gz.tbi")             , emit: somatic_sv_vcf_tbi
-    path "versions.yml"                                          , emit: versions
+    tuple val("${task.process}"), val("manta"), eval("configManta.py --version"), topic: versions, emit: versions_manta
 
     when:
     task.ext.when == null || task.ext.when
@@ -61,11 +61,6 @@ process MANTA_SOMATIC {
         ${prefix}.somatic_sv.vcf.gz
     mv manta/results/variants/somaticSV.vcf.gz.tbi \\
         ${prefix}.somatic_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 
     stub:
@@ -79,10 +74,5 @@ process MANTA_SOMATIC {
     touch ${prefix}.diploid_sv.vcf.gz.tbi
     echo "" | gzip > ${prefix}.somatic_sv.vcf.gz
     touch ${prefix}.somatic_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/manta/somatic/meta.yml b/modules/nf-core/manta/somatic/meta.yml
index 3d619dd586..048c1342cd 100644
--- a/modules/nf-core/manta/somatic/meta.yml
+++ b/modules/nf-core/manta/somatic/meta.yml
@@ -169,13 +169,29 @@ output:
           description: Index for gzipped VCF file containing variants
           pattern: "*.{vcf.gz.tbi}"
           ontologies: []
+  versions_manta:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@FriederikeHanssen"
   - "@nvnieuwk"
diff --git a/modules/nf-core/manta/tumoronly/environment.yml b/modules/nf-core/manta/tumoronly/environment.yml
index 3804c07f42..697c36d1c8 100644
--- a/modules/nf-core/manta/tumoronly/environment.yml
+++ b/modules/nf-core/manta/tumoronly/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::python=2.7.15
   - bioconda::manta=1.6.0
+  - conda-forge::python=2.7.15
diff --git a/modules/nf-core/manta/tumoronly/main.nf b/modules/nf-core/manta/tumoronly/main.nf
index b657468b58..ac3662b280 100644
--- a/modules/nf-core/manta/tumoronly/main.nf
+++ b/modules/nf-core/manta/tumoronly/main.nf
@@ -4,7 +4,7 @@ process MANTA_TUMORONLY {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f6/f696c93e6209e33ac0d15f1ecfa799bc67329eec07b0569e065ea8b220b53953/data' :
         'community.wave.seqera.io/library/manta_python:0eb71149179b3920' }"
 
@@ -21,7 +21,7 @@ process MANTA_TUMORONLY {
     tuple val(meta), path("*candidate_sv.vcf.gz.tbi")          , emit: candidate_sv_vcf_tbi
     tuple val(meta), path("*tumor_sv.vcf.gz")                  , emit: tumor_sv_vcf
     tuple val(meta), path("*tumor_sv.vcf.gz.tbi")              , emit: tumor_sv_vcf_tbi
-    path "versions.yml"                                        , emit: versions
+    tuple val("${task.process}"), val("manta"), eval("configManta.py --version"), topic: versions, emit: versions_manta
 
     when:
     task.ext.when == null || task.ext.when
@@ -54,11 +54,6 @@ process MANTA_TUMORONLY {
         ${prefix}.tumor_sv.vcf.gz
     mv manta/results/variants/tumorSV.vcf.gz.tbi \\
         ${prefix}.tumor_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 
     stub:
@@ -70,10 +65,5 @@ process MANTA_TUMORONLY {
     touch ${prefix}.candidate_sv.vcf.gz.tbi
     echo "" | gzip > ${prefix}.tumor_sv.vcf.gz
     touch ${prefix}.tumor_sv.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        manta: \$( configManta.py --version )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/manta/tumoronly/meta.yml b/modules/nf-core/manta/tumoronly/meta.yml
index 0773760b5a..b78a9e4844 100644
--- a/modules/nf-core/manta/tumoronly/meta.yml
+++ b/modules/nf-core/manta/tumoronly/meta.yml
@@ -137,13 +137,29 @@ output:
           description: Index for gzipped VCF file containing variants
           pattern: "*.{vcf.gz.tbi}"
           ontologies: []
+  versions_manta:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - manta:
+          type: string
+          description: The name of the tool
+      - configManta.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@maxulysse"
   - "@nvnieuwk"
diff --git a/modules/nf-core/strelka/germline/environment.yml b/modules/nf-core/strelka/germline/environment.yml
index ad1ff8498d..fbe5608cce 100644
--- a/modules/nf-core/strelka/germline/environment.yml
+++ b/modules/nf-core/strelka/germline/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::python=2.7.15
   - bioconda::strelka=2.9.10=h9ee0642_1
+  - conda-forge::python=2.7.15
diff --git a/modules/nf-core/strelka/germline/main.nf b/modules/nf-core/strelka/germline/main.nf
index 00499135c0..67e2680fd0 100644
--- a/modules/nf-core/strelka/germline/main.nf
+++ b/modules/nf-core/strelka/germline/main.nf
@@ -4,9 +4,9 @@ process STRELKA_GERMLINE {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/strelka:2.9.10--h9ee0642_1'
-        : 'biocontainers/strelka:2.9.10--h9ee0642_1'}"
+        : 'quay.io/biocontainers/strelka:2.9.10--h9ee0642_1'}"
 
     input:
     tuple val(meta), path(input), path(input_index), path(target_bed), path(target_bed_index)
@@ -18,7 +18,7 @@ process STRELKA_GERMLINE {
     tuple val(meta), path("*variants.vcf.gz.tbi"), emit: vcf_tbi
     tuple val(meta), path("*genome.vcf.gz"),       emit: genome_vcf
     tuple val(meta), path("*genome.vcf.gz.tbi"),   emit: genome_vcf_tbi
-    path "versions.yml",                           emit: versions
+    tuple val("${task.process}"), val('strelka'), eval("configureStrelkaGermlineWorkflow.py --version"), emit: versions_strelka, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -42,11 +42,6 @@ process STRELKA_GERMLINE {
     mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}.genome.vcf.gz.tbi
     mv strelka/results/variants/variants.vcf.gz     ${prefix}.variants.vcf.gz
     mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}.variants.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        strelka: \$( configureStrelkaGermlineWorkflow.py --version )
-    END_VERSIONS
     """
 
     stub:
@@ -56,10 +51,5 @@ process STRELKA_GERMLINE {
     touch ${prefix}.genome.vcf.gz.tbi
     echo "" | gzip > ${prefix}.variants.vcf.gz
     touch ${prefix}.variants.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        strelka: \$( configureStrelkaSomaticWorkflow.py --version )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/strelka/germline/meta.yml b/modules/nf-core/strelka/germline/meta.yml
index c20bf89a6a..4150d974b1 100644
--- a/modules/nf-core/strelka/germline/meta.yml
+++ b/modules/nf-core/strelka/germline/meta.yml
@@ -1,6 +1,6 @@
 name: strelka_germline
-description: Strelka2 is a fast and accurate small variant caller optimized for analysis
-  of germline variation
+description: Strelka2 is a fast and accurate small variant caller optimized for
+  analysis of germline variation
 keywords:
   - variantcalling
   - germline
@@ -9,13 +9,14 @@ keywords:
   - variants
 tools:
   - strelka:
-      description: Strelka calls somatic and germline small variants from mapped sequencing
-        reads
+      description: Strelka calls somatic and germline small variants from mapped
+        sequencing reads
       homepage: https://github.com/Illumina/strelka
       documentation: https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/README.md
       tool_dev_url: https://github.com/Illumina/strelka
       doi: 10.1038/s41592-018-0051-x
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: biotools:strelka
 input:
   - - meta:
@@ -40,7 +41,8 @@ input:
         ontologies: []
     - target_bed_index:
         type: file
-        description: Index for BED file containing target regions for variant calling
+        description: Index for BED file containing target regions for variant
+          calling
         pattern: "*.{bed.tbi}"
         ontologies: []
   - fasta:
@@ -99,13 +101,29 @@ output:
           description: index file for the genome_vcf file
           pattern: "*_genome.vcf.gz.tbi"
           ontologies: []
+  versions_strelka:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - strelka:
+          type: string
+          description: The name of the tool
+      - configureStrelkaGermlineWorkflow.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - strelka:
+          type: string
+          description: The name of the tool
+      - configureStrelkaGermlineWorkflow.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@arontommi"
 maintainers:
diff --git a/modules/nf-core/strelka/somatic/environment.yml b/modules/nf-core/strelka/somatic/environment.yml
index ad1ff8498d..fbe5608cce 100644
--- a/modules/nf-core/strelka/somatic/environment.yml
+++ b/modules/nf-core/strelka/somatic/environment.yml
@@ -4,5 +4,5 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - conda-forge::python=2.7.15
   - bioconda::strelka=2.9.10=h9ee0642_1
+  - conda-forge::python=2.7.15
diff --git a/modules/nf-core/strelka/somatic/main.nf b/modules/nf-core/strelka/somatic/main.nf
index 052cfee15f..8a30d11cb3 100644
--- a/modules/nf-core/strelka/somatic/main.nf
+++ b/modules/nf-core/strelka/somatic/main.nf
@@ -4,9 +4,9 @@ process STRELKA_SOMATIC {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/strelka:2.9.10--h9ee0642_1'
-        : 'biocontainers/strelka:2.9.10--h9ee0642_1'}"
+        : 'quay.io/biocontainers/strelka:2.9.10--h9ee0642_1'}"
 
     input:
     tuple val(meta), path(input_normal), path(input_index_normal), path(input_tumor), path(input_index_tumor), path(manta_candidate_small_indels), path(manta_candidate_small_indels_tbi), path(target_bed), path(target_bed_index)
@@ -18,7 +18,7 @@ process STRELKA_SOMATIC {
     tuple val(meta), path("*.somatic_indels.vcf.gz.tbi"), emit: vcf_indels_tbi
     tuple val(meta), path("*.somatic_snvs.vcf.gz"),       emit: vcf_snvs
     tuple val(meta), path("*.somatic_snvs.vcf.gz.tbi"),   emit: vcf_snvs_tbi
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('strelka'), eval("configureStrelkaSomaticWorkflow.py --version"), emit: versions_strelka, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -45,11 +45,6 @@ process STRELKA_SOMATIC {
     mv strelka/results/variants/somatic.indels.vcf.gz.tbi ${prefix}.somatic_indels.vcf.gz.tbi
     mv strelka/results/variants/somatic.snvs.vcf.gz       ${prefix}.somatic_snvs.vcf.gz
     mv strelka/results/variants/somatic.snvs.vcf.gz.tbi   ${prefix}.somatic_snvs.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        strelka: \$( configureStrelkaSomaticWorkflow.py --version )
-    END_VERSIONS
     """
 
     stub:
@@ -59,10 +54,5 @@ process STRELKA_SOMATIC {
     touch ${prefix}.somatic_indels.vcf.gz.tbi
     echo "" | gzip > ${prefix}.somatic_snvs.vcf.gz
     touch ${prefix}.somatic_snvs.vcf.gz.tbi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        strelka: \$( configureStrelkaSomaticWorkflow.py --version )
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/strelka/somatic/meta.yml b/modules/nf-core/strelka/somatic/meta.yml
index eca9ea43f2..aeb3a8080a 100644
--- a/modules/nf-core/strelka/somatic/meta.yml
+++ b/modules/nf-core/strelka/somatic/meta.yml
@@ -1,7 +1,7 @@
 name: strelka_somatic
-description: Strelka2 is a fast and accurate small variant caller optimized for analysis
-  of germline variation in small cohorts and somatic variation in tumor/normal sample
-  pairs
+description: Strelka2 is a fast and accurate small variant caller optimized for
+  analysis of germline variation in small cohorts and somatic variation in
+  tumor/normal sample pairs
 keywords:
   - variant calling
   - germline
@@ -10,13 +10,14 @@ keywords:
   - variants
 tools:
   - strelka:
-      description: Strelka calls somatic and germline small variants from mapped sequencing
-        reads
+      description: Strelka calls somatic and germline small variants from mapped
+        sequencing reads
       homepage: https://github.com/Illumina/strelka
       documentation: https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/README.md
       tool_dev_url: https://github.com/Illumina/strelka
       doi: 10.1038/s41592-018-0051-x
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: biotools:strelka
 input:
   - - meta:
@@ -61,7 +62,8 @@ input:
         ontologies: []
     - target_bed_index:
         type: file
-        description: Index for BED file containing target regions for variant calling
+        description: Index for BED file containing target regions for variant
+          calling
         pattern: "*.{bed.tbi}"
         ontologies: []
   - fasta:
@@ -119,13 +121,29 @@ output:
           description: Index for gzipped VCF file containing variants
           pattern: "*.{vcf.gz.tbi}"
           ontologies: []
+  versions_strelka:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - strelka:
+          type: string
+          description: The name of the tool
+      - configureStrelkaSomaticWorkflow.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - strelka:
+          type: string
+          description: The name of the tool
+      - configureStrelkaSomaticWorkflow.py --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@drpatelh"
 maintainers:
diff --git a/modules/nf-core/svdb/merge/environment.yml b/modules/nf-core/svdb/merge/environment.yml
index dc587136eb..00d472a335 100644
--- a/modules/nf-core/svdb/merge/environment.yml
+++ b/modules/nf-core/svdb/merge/environment.yml
@@ -3,7 +3,6 @@
 channels:
   - conda-forge
   - bioconda
-
 dependencies:
-  - bcftools=1.21
-  - svdb=2.8.2
+  - bioconda::bcftools=1.23=*
+  - bioconda::svdb=2.8.4
diff --git a/modules/nf-core/svdb/merge/main.nf b/modules/nf-core/svdb/merge/main.nf
index 104f5ad799..3963f64d1e 100644
--- a/modules/nf-core/svdb/merge/main.nf
+++ b/modules/nf-core/svdb/merge/main.nf
@@ -1,10 +1,11 @@
 process SVDB_MERGE {
     tag "$meta.id"
     label 'process_single'
+
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-375a758a4ca8c128fb9d38047a68a9f4322d2acd:b3615e06ef17566f2988a215ce9e10808c1d08bf-0':
-        'biocontainers/mulled-v2-375a758a4ca8c128fb9d38047a68a9f4322d2acd:b3615e06ef17566f2988a215ce9e10808c1d08bf-0' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/f5/f59712ead354411dd8bea4918d777737ca4ef2ad1360289507fe35acb688e74f/data':
+        'community.wave.seqera.io/library/bcftools_svdb:12db401acbacc624' }"
 
     input:
     tuple val(meta), path(vcfs)
@@ -15,7 +16,8 @@ process SVDB_MERGE {
     tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf
     tuple val(meta), path("*.tbi")                    , emit: tbi, optional: true
     tuple val(meta), path("*.csi")                    , emit: csi, optional: true
-    path "versions.yml"                               , emit: versions
+    tuple val("${task.process}"), val('svdb'), eval("svdb | sed -nE 's/.*SVDB-([0-9.]+).*/\\1/p'"), emit: versions_svdb, topic: versions
+    tuple val("${task.process}"), val('bcftools'), eval("bcftools --version | sed '1!d; s/^.*bcftools //'"), emit: versions_bcftools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -29,31 +31,28 @@ process SVDB_MERGE {
     if (input_priority && vcfs.collect().size() != input_priority.collect().size()) {
         error "If priority is used, one tag per VCF is needed"
     }
+    // Convert to list if not already for simpler logic below
+    def vcf_list = vcfs instanceof List ? vcfs : [vcfs]
 
-    def input = ""
-    def prio = ""
     if (input_priority) {
-        if (vcfs.collect().size() > 1 && sort_inputs) {
-            // make vcf-priority pairs and sort on VCF name, so priority is also sorted the same
-            def pairs = vcfs.indices.collect { [vcfs[it], input_priority[it]] }
-            pairs = pairs.sort { a, b -> a[0].name <=> b[0].name }
-            vcfs = pairs.collect { it[0] }
-            priority = pairs.collect { it[1] }
-        } else {
-            priority = input_priority
+        pairs = vcf_list.indices.collect { index -> [vcf_list[index], input_priority[index]] }
+
+        if(sort_inputs) {
+            pairs.sort { a, b -> a[0].name <=> b[0].name }
         }
 
-        // Build inputs
         prio = "--priority ${input_priority.join(',')}"
-        input = vcfs
-            .withIndex()
-            .collect { vcf, index -> "${vcf}:${priority[index]}" }
-            .join(" ")
-
+        input = pairs.collect { vcf, priority -> "${vcf}:${priority}"}
     } else {
-        // if there's no priority input just sort the vcfs by name if possible
-        input = (vcfs.collect().size() > 1 && sort_inputs) ? vcfs.sort { it.name } : vcfs
+        if (sort_inputs) {
+            vcf_list.sort { vcf_file -> vcf_file.name }
+        }
+
+        prio = ""
+        input = vcf_list
     }
+    // Convert from list to string
+    input = input.join(' ')
 
     def extension = args2.contains("--output-type b") || args2.contains("-Ob") ? "bcf.gz" :
                     args2.contains("--output-type u") || args2.contains("-Ou") ? "bcf" :
@@ -71,11 +70,6 @@ process SVDB_MERGE {
             --threads ${task.cpus} \\
             --output ${prefix}.${extension}
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        svdb: \$( echo \$(svdb) | head -1 | sed 's/usage: SVDB-\\([0-9]\\.[0-9]\\.[0-9]\\).*/\\1/' )
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -96,10 +90,5 @@ process SVDB_MERGE {
     ${create_cmd} ${prefix}.${extension}
     ${create_index}
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        svdb: \$( echo \$(svdb) | head -1 | sed 's/usage: SVDB-\\([0-9]\\.[0-9]\\.[0-9]\\).*/\\1/' )
-        bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/svdb/merge/meta.yml b/modules/nf-core/svdb/merge/meta.yml
index c34a9cb157..48d73e47ec 100644
--- a/modules/nf-core/svdb/merge/meta.yml
+++ b/modules/nf-core/svdb/merge/meta.yml
@@ -1,5 +1,6 @@
 name: svdb_merge
-description: The merge module merges structural variants within one or more vcf files.
+description: The merge module merges structural variants within one or more vcf
+  files.
 keywords:
   - structural variants
   - vcf
@@ -23,20 +24,20 @@ input:
           One or more VCF files. The order and number of files should correspond to
           the order and number of tags in the `priority` input channel.
         pattern: "*.{vcf,vcf.gz}"
-  - - input_priority:
-        type: list
-        description: |
-          Prioritize the input VCF files according to this list,
-          e.g ['tiddit','cnvnator']. The order and number of tags should correspond to
-          the order and number of VCFs in the `vcfs` input channel.
-  - - sort_inputs:
-        type: boolean
-        description: |
-          Should the input files be sorted by name. The priority tag will be sorted
-          together with it's corresponding VCF file.
+  - input_priority:
+      type: list
+      description: |
+        Prioritize the input VCF files according to this list,
+        e.g ['tiddit','cnvnator']. The order and number of tags should correspond to
+        the order and number of VCFs in the `vcfs` input channel.
+  - sort_inputs:
+      type: boolean
+      description: |
+        Should the input files be sorted by name. The priority tag will be sorted
+        together with it's corresponding VCF file.
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -45,8 +46,9 @@ output:
           type: file
           description: VCF output file
           pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}"
-  - tbi:
-      - meta:
+          ontologies: []
+  tbi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -55,8 +57,9 @@ output:
           type: file
           description: Alternative VCF file index
           pattern: "*.tbi"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -65,11 +68,47 @@ output:
           type: file
           description: Default VCF file index
           pattern: "*.csi"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_svdb:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - svdb:
+          type: string
+          description: The name of the tool
+      - svdb | sed -nE 's/.*SVDB-([0-9.]+).*/\1/p':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_bcftools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - svdb:
+          type: string
+          description: The name of the tool
+      - svdb | sed -nE 's/.*SVDB-([0-9.]+).*/\1/p':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bcftools:
+          type: string
+          description: The tool name
+      - bcftools --version | sed '1!d; s/^.*bcftools //':
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@ramprasadn"
 maintainers:
diff --git a/modules/nf-core/tiddit/sv/environment.yml b/modules/nf-core/tiddit/sv/environment.yml
index a33b14c85f..d00f5e3630 100644
--- a/modules/nf-core/tiddit/sv/environment.yml
+++ b/modules/nf-core/tiddit/sv/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::tiddit=3.6.1
+  - bioconda::tiddit=3.9.5
diff --git a/modules/nf-core/tiddit/sv/main.nf b/modules/nf-core/tiddit/sv/main.nf
index f350e31443..fd0af3dd26 100644
--- a/modules/nf-core/tiddit/sv/main.nf
+++ b/modules/nf-core/tiddit/sv/main.nf
@@ -3,54 +3,44 @@ process TIDDIT_SV {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/tiddit:3.6.1--py38h24c8ff8_0' :
-        'biocontainers/tiddit:3.6.1--py38h24c8ff8_0' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/6a/6a427ef9929eb787b83224b3c8dd5d1dd7f7897e6921c60ecc5e58ef705daf6b/data' :
+        'community.wave.seqera.io/library/tiddit:3.9.5--3fb6c287f34e6ab0' }"
 
     input:
     tuple val(meta), path(input), path(input_index)
-    tuple val(meta2), path(fasta)
+    tuple val(meta2), path(fasta), path(fai)
     tuple val(meta3), path(bwa_index)
 
     output:
-    tuple val(meta), path("*.vcf")         , emit: vcf
-    tuple val(meta), path("*.ploidies.tab"), emit: ploidy
-    path  "versions.yml"                   , emit: versions
+    tuple val(meta), path("${prefix}.vcf")         , emit: vcf
+    tuple val(meta), path("${prefix}.ploidies.tab"), emit: ploidy
+    tuple val("${task.process}"), val('tiddit'), eval("tiddit | sed -n 's/^usage: tiddit-//; s/ .*//p'"), topic: versions, emit: versions_tiddit
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
     def args = task.ext.args ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def bwa_command = bwa_index ? "[[ -d $bwa_index ]] && for i in $bwa_index/*; do [[ -f $fasta && ! \"\$i\" =~ .*\"$fasta.\".* ]] && ln -s \$i ${fasta}.\${i##*.} || ln -s \$i .; done" : ""
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def bwa_command = bwa_index ? "[[ -d ${bwa_index} ]] && for i in ${bwa_index}/*; do [[ -f ${fasta} && ! \"\$i\" =~ .*\"${fasta}.\".* ]] && ln -s \$i ${fasta}.\${i##*.} || ln -s \$i .; done" : ""
 
     """
     $bwa_command
 
     tiddit \\
         --sv \\
-        $args \\
+        ${args} \\
         --threads $task.cpus \\
-        --bam $input \\
-        --ref $fasta \\
-        -o $prefix
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
-    END_VERSIONS
+        --bam ${input} \\
+        --ref ${fasta} \\
+        -o ${prefix}
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
+    prefix = task.ext.prefix ?: "${meta.id}"
     """
-    touch ${prefix}.vcf
-    touch ${prefix}.ploidies.tab
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tiddit: \$(echo \$(tiddit 2>&1) | sed 's/^.*tiddit-//; s/ .*\$//')
-    END_VERSIONS
+    echo "" > ${prefix}.vcf
+    echo "" > ${prefix}.ploidies.tab
     """
 }
diff --git a/modules/nf-core/tiddit/sv/meta.yml b/modules/nf-core/tiddit/sv/meta.yml
index 21527baf13..78232760c1 100644
--- a/modules/nf-core/tiddit/sv/meta.yml
+++ b/modules/nf-core/tiddit/sv/meta.yml
@@ -10,7 +10,8 @@ tools:
       homepage: https://github.com/SciLifeLab/TIDDIT
       documentation: https://github.com/SciLifeLab/TIDDIT/blob/master/README.md
       doi: 10.12688/f1000research.11168.1
-      licence: ["GPL-3.0-or-later"]
+      licence:
+        - "GPL-3.0-or-later"
       identifier: biotools:tiddit
 input:
   - - meta:
@@ -22,10 +23,12 @@ input:
         type: file
         description: BAM/CRAM file
         pattern: "*.{bam,cram}"
+        ontologies: []
     - input_index:
         type: file
         description: BAM/CRAM index file
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -35,6 +38,12 @@ input:
         type: file
         description: Input FASTA file
         pattern: "*.{fasta,fa}"
+        ontologies: []
+    - fai:
+        type: file
+        description: Input FASTA index file
+        pattern: "*.{fai}"
+        ontologies: []
   - - meta3:
         type: map
         description: |
@@ -44,32 +53,51 @@ input:
         type: file
         description: BWA genome index files
         pattern: "Directory containing BWA index *.{amb,ann,bwt,pac,sa}"
+        ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.vcf":
+      - "${prefix}.vcf":
           type: file
           description: vcf
           pattern: "*.{vcf}"
-  - ploidy:
-      - meta:
+          ontologies: []
+  ploidy:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.ploidies.tab":
+      - "${prefix}.ploidies.tab":
           type: file
           description: tab
           pattern: "*.{ploidies.tab}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_tiddit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - tiddit:
+          type: string
+          description: The name of the tool
+      - "tiddit | sed -n 's/^usage: tiddit-//; s/ .*//p'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - tiddit:
+          type: string
+          description: The name of the tool
+      - "tiddit | sed -n 's/^usage: tiddit-//; s/ .*//p'":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@maxulysse"
 maintainers:
diff --git a/modules/nf-core/vcflib/vcffilter/main.nf b/modules/nf-core/vcflib/vcffilter/main.nf
index 9a375df3fd..1df008acee 100644
--- a/modules/nf-core/vcflib/vcffilter/main.nf
+++ b/modules/nf-core/vcflib/vcffilter/main.nf
@@ -3,7 +3,7 @@ process VCFLIB_VCFFILTER {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/fc/fc33d59c090cef123aca26ae17fbddbd596640304d8325cbd5816229fa2c05ee/data'
         : 'community.wave.seqera.io/library/vcflib:1.0.14--cc8ffb2c1a080797'}"
 
@@ -12,7 +12,8 @@ process VCFLIB_VCFFILTER {
 
     output:
     tuple val(meta), path("*.vcf.gz"), emit: vcf
-    path "versions.yml",               emit: versions
+    tuple val("${task.process}"), val('vcflib'), val("1.0.14"), topic: versions, emit: versions_vcflib
+    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
 
     when:
     task.ext.when == null || task.ext.when
@@ -21,8 +22,7 @@ process VCFLIB_VCFFILTER {
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}.filter"
-    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def VERSION = '1.0.14'
+
     if (!(args.contains("-f") || args.contains("--info-filter") || args.contains("-g") || args.contains("--genotype-filter"))) {
         error("VCFLIB_VCFFILTER requires either the -f/--info-filter or -g/--genotype-filter arguments to be supplied using ext.args.")
     }
@@ -34,26 +34,15 @@ process VCFLIB_VCFFILTER {
         ${args} \\
         ${vcf} \\
         | bgzip -c ${args2} > ${prefix}.vcf.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        vcflib: ${VERSION}
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}.filter"
-    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
-    def VERSION = '1.0.14'
+
     if ("${vcf}" == "${prefix}.vcf.gz") {
         error("Input and output names are the same, set prefix in module configuration to disambiguate!")
     }
     """
     echo | gzip > ${prefix}.vcf.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        vcflib: ${VERSION}
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/vcflib/vcffilter/meta.yml b/modules/nf-core/vcflib/vcffilter/meta.yml
index 3e51ce3531..4082514658 100644
--- a/modules/nf-core/vcflib/vcffilter/meta.yml
+++ b/modules/nf-core/vcflib/vcffilter/meta.yml
@@ -12,7 +12,8 @@ tools:
       documentation: "https://github.com/vcflib/vcflib"
       tool_dev_url: "https://github.com/vcflib/vcflib"
       doi: "10.1371/journal.pcbi.1009123"
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:vcflib
 input:
   - - meta:
@@ -42,13 +43,27 @@ output:
           description: Filtered VCF file
           pattern: "*.{vcf.gz}"
           ontologies: []
+  versions_vcflib:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - vcflib:
+          type: string
+          description: The name of the tool
+      - 1.0.14:
+          type: string
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - vcflib:
+          type: string
+          description: The name of the tool
+      - 1.0.14:
+          type: string
+          description: The expression to obtain the version of the tool
 authors:
   - "@zachary-foster"
 maintainers:
diff --git a/modules/nf-core/vcftools/environment.yml b/modules/nf-core/vcftools/environment.yml
index ff0e9d03cd..121a7b865a 100644
--- a/modules/nf-core/vcftools/environment.yml
+++ b/modules/nf-core/vcftools/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::vcftools=0.1.16
+  - bioconda::vcftools=0.1.17
diff --git a/modules/nf-core/vcftools/main.nf b/modules/nf-core/vcftools/main.nf
index 67bca9af0c..ad5e08b513 100644
--- a/modules/nf-core/vcftools/main.nf
+++ b/modules/nf-core/vcftools/main.nf
@@ -3,9 +3,9 @@ process VCFTOOLS {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/vcftools:0.1.16--he513fc3_4' :
-        'biocontainers/vcftools:0.1.16--he513fc3_4' }"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/vcftools:0.1.17--pl5321h077b44d_0' :
+        'quay.io/biocontainers/vcftools:0.1.17--pl5321h077b44d_0' }"
 
     input:
     // Owing to the nature of vcftools we here provide solutions to working with optional bed files and optional
@@ -79,7 +79,7 @@ process VCFTOOLS {
     tuple val(meta), path("*.diff.indv")              , optional:true, emit: diff_indv
     tuple val(meta), path("*.diff.discordance.matrix"), optional:true, emit: diff_discd_matrix
     tuple val(meta), path("*.diff.switch")            , optional:true, emit: diff_switch_error
-    path "versions.yml"                               , emit: versions
+    tuple val("${task.process}"), val('vcftools'), eval('vcftools --version 2>&1 | sed "s/^.*VCFtools (//;s/).*//"'), emit: versions_vcftools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -94,18 +94,18 @@ process VCFTOOLS {
         (args.contains('--hapcount')) ? "--hapcount ${bed}" :
         (args.contains('--positions')) ? "--positions ${bed}" :
         (args.contains('--exclude-positions')) ? "--exclude-positions ${bed}"  : ''
-    args_list.removeIf { it.contains('--bed') }
-    args_list.removeIf { it.contains('--exclude-bed') }
-    args_list.removeIf { it.contains('--hapcount') }
-    args_list.removeIf { it.contains('--positions') }
-    args_list.removeIf { it.contains('--exclude-positions') }
+    args_list.removeIf { arg -> arg.contains('--bed') }
+    args_list.removeIf { arg -> arg.contains('--exclude-bed') }
+    args_list.removeIf { arg -> arg.contains('--hapcount') }
+    args_list.removeIf { arg -> arg.contains('--positions') }
+    args_list.removeIf { arg -> arg.contains('--exclude-positions') }
 
     def diff_variant_arg = (args.contains('--diff')) ? "--diff ${diff_variant_file}" :
         (args.contains('--gzdiff')) ? "--gzdiff ${diff_variant_file}" :
         (args.contains('--diff-bcf')) ? "--diff-bcf ${diff_variant_file}" : ''
-    args_list.removeIf { it.contains('--diff') }
-    args_list.removeIf { it.contains('--gzdiff') }
-    args_list.removeIf { it.contains('--diff-bcf') }
+    args_list.removeIf { arg -> arg.contains('--diff') }
+    args_list.removeIf { arg -> arg.contains('--gzdiff') }
+    args_list.removeIf { arg -> arg.contains('--diff-bcf') }
 
     def input_file = ("$variant_file".endsWith(".vcf")) ? "--vcf ${variant_file}" :
         ("$variant_file".endsWith(".vcf.gz")) ? "--gzvcf ${variant_file}" :
@@ -118,11 +118,6 @@ process VCFTOOLS {
         ${args_list.join(' ')} \\
         $bed_arg \\
         $diff_variant_arg
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        vcftools: \$(echo \$(vcftools --version 2>&1) | sed 's/^.*VCFtools (//;s/).*//')
-    END_VERSIONS
     """
 
     stub:
@@ -190,10 +185,5 @@ process VCFTOOLS {
     touch ${prefix}.diff.indv
     touch ${prefix}.diff.discordance.matrix
     touch ${prefix}.diff.switch
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        vcftools: \$(echo \$(vcftools --version 2>&1) | sed 's/^.*VCFtools (//;s/).*//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/vcftools/meta.yml b/modules/nf-core/vcftools/meta.yml
index b9bae4df12..3e663da0b7 100644
--- a/modules/nf-core/vcftools/meta.yml
+++ b/modules/nf-core/vcftools/meta.yml
@@ -6,12 +6,13 @@ keywords:
   - sort
 tools:
   - vcftools:
-      description: A set of tools written in Perl and C++ for working with VCF files.
-        This package only contains the C++ libraries whereas the package perl-vcftools-vcf
-        contains the perl libraries
+      description: A set of tools written in Perl and C++ for working with VCF
+        files. This package only contains the C++ libraries whereas the package
+        perl-vcftools-vcf contains the perl libraries
       homepage: http://vcftools.sourceforge.net/
       documentation: http://vcftools.sourceforge.net/man_latest.html
-      licence: ["LGPL"]
+      licence:
+        - "LGPL"
       identifier: biotools:vcftools
 input:
   - - meta:
@@ -22,17 +23,20 @@ input:
     - variant_file:
         type: file
         description: variant input file which can be vcf, vcf.gz, or bcf format.
-  - - bed:
-        type: file
-        description: bed file which can be used with different arguments in vcftools
-          (optional)
-  - - diff_variant_file:
-        type: file
-        description: secondary variant file which can be used with the 'diff' suite
-          of tools (optional)
+        ontologies: []
+  - bed:
+      type: file
+      description: bed file which can be used with different arguments in vcftools
+        (optional)
+      ontologies: []
+  - diff_variant_file:
+      type: file
+      description: secondary variant file which can be used with the 'diff' suite
+        of tools (optional)
+      ontologies: []
 output:
-  - vcf:
-      - meta:
+  vcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -41,8 +45,9 @@ output:
           type: file
           description: vcf file (optional)
           pattern: "*.vcf"
-  - bcf:
-      - meta:
+          ontologies: []
+  bcf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,8 +56,9 @@ output:
           type: file
           description: bcf file (optional)
           pattern: "*.bcf"
-  - frq:
-      - meta:
+          ontologies: []
+  frq:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -61,8 +67,9 @@ output:
           type: file
           description: Allele frequency for each site (optional)
           pattern: "*.frq"
-  - frq_count:
-      - meta:
+          ontologies: []
+  frq_count:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,8 +78,9 @@ output:
           type: file
           description: Allele counts for each site (optional)
           pattern: "*.frq.count"
-  - idepth:
-      - meta:
+          ontologies: []
+  idepth:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,8 +89,9 @@ output:
           type: file
           description: mean depth per individual (optional)
           pattern: "*.idepth"
-  - ldepth:
-      - meta:
+          ontologies: []
+  ldepth:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,18 +100,21 @@ output:
           type: file
           description: depth per site summed across individuals (optional)
           pattern: "*.ildepth"
-  - ldepth_mean:
-      - meta:
+          ontologies: []
+  ldepth_mean:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.ldepth.mean":
           type: file
-          description: mean depth per site calculated across individuals (optional)
+          description: mean depth per site calculated across individuals
+            (optional)
           pattern: "*.ldepth.mean"
-  - gdepth:
-      - meta:
+          ontologies: []
+  gdepth:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -111,8 +123,9 @@ output:
           type: file
           description: depth for each genotype in vcf file (optional)
           pattern: "*.gdepth"
-  - hap_ld:
-      - meta:
+          ontologies: []
+  hap_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -121,83 +134,94 @@ output:
           type: file
           description: r2, D, and D’ statistics using phased haplotypes (optional)
           pattern: "*.hap.ld"
-  - geno_ld:
-      - meta:
+          ontologies: []
+  geno_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.geno.ld":
           type: file
-          description: squared correlation coefficient between genotypes encoded as 0,
-            1 and 2 to represent the number of non-reference alleles in each individual
-            (optional)
+          description: squared correlation coefficient between genotypes encoded
+            as 0, 1 and 2 to represent the number of non-reference alleles in each
+            individual (optional)
           pattern: "*.geno.ld"
-  - geno_chisq:
-      - meta:
+          ontologies: []
+  geno_chisq:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.geno.chisq":
           type: file
-          description: test for genotype independence via the chi-squared statistic (optional)
+          description: test for genotype independence via the chi-squared
+            statistic (optional)
           pattern: "*.geno.chisq"
-  - list_hap_ld:
-      - meta:
+          ontologies: []
+  list_hap_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.list.hap.ld":
           type: file
-          description: r2 statistics of the sites contained in the provided input file
-            verses all other sites (optional)
+          description: r2 statistics of the sites contained in the provided input
+            file verses all other sites (optional)
           pattern: "*.list.hap.ld"
-  - list_geno_ld:
-      - meta:
+          ontologies: []
+  list_geno_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.list.geno.ld":
           type: file
-          description: r2 statistics of the sites contained in the provided input file
-            verses all other sites (optional)
+          description: r2 statistics of the sites contained in the provided input
+            file verses all other sites (optional)
           pattern: "*.list.geno.ld"
-  - interchrom_hap_ld:
-      - meta:
+          ontologies: []
+  interchrom_hap_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.interchrom.hap.ld":
           type: file
-          description: r2 statistics for sites (haplotypes) on different chromosomes (optional)
+          description: r2 statistics for sites (haplotypes) on different
+            chromosomes (optional)
           pattern: "*.interchrom.hap.ld"
-  - interchrom_geno_ld:
-      - meta:
+          ontologies: []
+  interchrom_geno_ld:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.interchrom.geno.ld":
           type: file
-          description: r2 statistics for sites (genotypes) on different chromosomes (optional)
+          description: r2 statistics for sites (genotypes) on different
+            chromosomes (optional)
           pattern: "*.interchrom.geno.ld"
-  - tstv:
-      - meta:
+          ontologies: []
+  tstv:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.TsTv":
           type: file
-          description: Transition / Transversion ratio in bins of size defined in options
-            (optional)
+          description: Transition / Transversion ratio in bins of size defined in
+            options (optional)
           pattern: "*.TsTv"
-  - tstv_summary:
-      - meta:
+          ontologies: []
+  tstv_summary:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -206,41 +230,45 @@ output:
           type: file
           description: Summary of all Transitions and Transversions (optional)
           pattern: "*.TsTv.summary"
-  - tstv_count:
-      - meta:
+          ontologies: []
+  tstv_count:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.TsTv.count":
           type: file
-          description: Transition / Transversion ratio as a function of alternative allele
-            count (optional)
+          description: Transition / Transversion ratio as a function of
+            alternative allele count (optional)
           pattern: "*.TsTv.count"
-  - tstv_qual:
-      - meta:
+          ontologies: []
+  tstv_qual:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.TsTv.qual":
           type: file
-          description: Transition / Transversion ratio as a function of SNP quality threshold
-            (optional)
+          description: Transition / Transversion ratio as a function of SNP
+            quality threshold (optional)
           pattern: "*.TsTv.qual"
-  - filter_summary:
-      - meta:
+          ontologies: []
+  filter_summary:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.FILTER.summary":
           type: file
-          description: Summary of the number of SNPs and Ts/Tv ratio for each FILTER category
-            (optional)
+          description: Summary of the number of SNPs and Ts/Tv ratio for each
+            FILTER category (optional)
           pattern: "*.FILTER.summary"
-  - sites_pi:
-      - meta:
+          ontologies: []
+  sites_pi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -249,29 +277,33 @@ output:
           type: file
           description: Nucleotide divergency on a per-site basis (optional)
           pattern: "*.sites.pi"
-  - windowed_pi:
-      - meta:
+          ontologies: []
+  windowed_pi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.windowed.pi":
           type: file
-          description: Nucleotide diversity in windows, with window size determined by
-            options (optional)
+          description: Nucleotide diversity in windows, with window size
+            determined by options (optional)
           pattern: "*windowed.pi"
-  - weir_fst:
-      - meta:
+          ontologies: []
+  weir_fst:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.weir.fst":
           type: file
-          description: Fst estimate from Weir and Cockerham’s 1984 paper (optional)
+          description: Fst estimate from Weir and Cockerham’s 1984 paper
+            (optional)
           pattern: "*.weir.fst"
-  - heterozygosity:
-      - meta:
+          ontologies: []
+  heterozygosity:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -280,41 +312,46 @@ output:
           type: file
           description: Heterozygosity on a per-individual basis (optional)
           pattern: "*.het"
-  - hwe:
-      - meta:
+          ontologies: []
+  hwe:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.hwe":
           type: file
-          description: Contains the Observed numbers of Homozygotes and Heterozygotes
-            and the corresponding Expected numbers under HWE (optional)
+          description: Contains the Observed numbers of Homozygotes and
+            Heterozygotes and the corresponding Expected numbers under HWE
+            (optional)
           pattern: "*.hwe"
-  - tajima_d:
-      - meta:
+          ontologies: []
+  tajima_d:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.Tajima.D":
           type: file
-          description: Tajima’s D statistic in bins with size of the specified number
-            in options (optional)
+          description: Tajima’s D statistic in bins with size of the specified
+            number in options (optional)
           pattern: "*.Tajima.D"
-  - freq_burden:
-      - meta:
+          ontologies: []
+  freq_burden:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.ifreqburden":
           type: file
-          description: Number of variants within each individual of a specific frequency
-            in options (optional)
+          description: Number of variants within each individual of a specific
+            frequency in options (optional)
           pattern: "*.ifreqburden"
-  - lroh:
-      - meta:
+          ontologies: []
+  lroh:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -323,30 +360,34 @@ output:
           type: file
           description: Long Runs of Homozygosity (optional)
           pattern: "*.LROH"
-  - relatedness:
-      - meta:
+          ontologies: []
+  relatedness:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.relatedness":
           type: file
-          description: Relatedness statistic based on the method of Yang et al, Nature
-            Genetics 2010 (doi:10.1038/ng.608) (optional)
+          description: Relatedness statistic based on the method of Yang et al,
+            Nature Genetics 2010 (doi:10.1038/ng.608) (optional)
           pattern: "*.relatedness"
-  - relatedness2:
-      - meta:
+          ontologies: []
+  relatedness2:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.relatedness2":
           type: file
-          description: Relatedness statistic based on the method of Manichaikul et al.,
-            BIOINFORMATICS 2010 (doi:10.1093/bioinformatics/btq559) (optional)
+          description: Relatedness statistic based on the method of Manichaikul et
+            al., BIOINFORMATICS 2010 (doi:10.1093/bioinformatics/btq559)
+            (optional)
           pattern: "*.relatedness2"
-  - lqual:
-      - meta:
+          ontologies: []
+  lqual:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -355,8 +396,9 @@ output:
           type: file
           description: per-site SNP quality (optional)
           pattern: "*.lqual"
-  - missing_individual:
-      - meta:
+          ontologies: []
+  missing_individual:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -365,8 +407,9 @@ output:
           type: file
           description: Missingness on a per-individual basis (optional)
           pattern: "*.imiss"
-  - missing_site:
-      - meta:
+          ontologies: []
+  missing_site:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -375,18 +418,21 @@ output:
           type: file
           description: Missingness on a per-site basis (optional)
           pattern: "*.lmiss"
-  - snp_density:
-      - meta:
+          ontologies: []
+  snp_density:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.snpden":
           type: file
-          description: Number and density of SNPs in bins of size defined by option (optional)
+          description: Number and density of SNPs in bins of size defined by
+            option (optional)
           pattern: "*.snpden"
-  - kept_sites:
-      - meta:
+          ontologies: []
+  kept_sites:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -395,8 +441,9 @@ output:
           type: file
           description: All sites that have been kept after filtering (optional)
           pattern: "*.kept.sites"
-  - removed_sites:
-      - meta:
+          ontologies: []
+  removed_sites:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -405,28 +452,33 @@ output:
           type: file
           description: All sites that have been removed after filtering (optional)
           pattern: "*.removed.sites"
-  - singeltons:
-      - meta:
+          ontologies: []
+  singeltons:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.singletons":
           type: file
-          description: Location of singletons, and the individual they occur in (optional)
+          description: Location of singletons, and the individual they occur in
+            (optional)
           pattern: "*.singeltons"
-  - indel_hist:
-      - meta:
+          ontologies: []
+  indel_hist:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.indel.hist":
           type: file
-          description: Histogram file of the length of all indels (including SNPs) (optional)
+          description: Histogram file of the length of all indels (including SNPs)
+            (optional)
           pattern: "*.indel_hist"
-  - hapcount:
-      - meta:
+          ontologies: []
+  hapcount:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -435,8 +487,9 @@ output:
           type: file
           description: Unique haplotypes within user specified bins (optional)
           pattern: "*.hapcount"
-  - mendel:
-      - meta:
+          ontologies: []
+  mendel:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -445,29 +498,33 @@ output:
           type: file
           description: Mendel errors identified in trios (optional)
           pattern: "*.mendel"
-  - format:
-      - meta:
+          ontologies: []
+  format:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.FORMAT":
           type: file
-          description: Extracted information from the genotype fields in the VCF file
-            relating to a specified FORMAT identifier (optional)
+          description: Extracted information from the genotype fields in the VCF
+            file relating to a specified FORMAT identifier (optional)
           pattern: "*.FORMAT"
-  - info:
-      - meta:
+          ontologies: []
+  info:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.INFO":
           type: file
-          description: Extracted information from the INFO field in the VCF file (optional)
+          description: Extracted information from the INFO field in the VCF file
+            (optional)
           pattern: "*.INFO"
-  - genotypes_matrix:
-      - meta:
+          ontologies: []
+  genotypes_matrix:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -480,40 +537,45 @@ output:
             Genotypes are represented as 0, 1 and 2, where the number represent that number of non-reference alleles.
             Missing genotypes are represented by -1 (optional)
           pattern: "*.012"
-  - genotypes_matrix_individual:
-      - meta:
+          ontologies: []
+  genotypes_matrix_individual:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.012.indv":
           type: file
-          description: Details the individuals included in the main genotypes_matrix file
-            (optional)
+          description: Details the individuals included in the main
+            genotypes_matrix file (optional)
           pattern: "*.012.indv"
-  - genotypes_matrix_position:
-      - meta:
+          ontologies: []
+  genotypes_matrix_position:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.012.pos":
           type: file
-          description: Details the site locations included in the main genotypes_matrix
-            file (optional)
+          description: Details the site locations included in the main
+            genotypes_matrix file (optional)
           pattern: "*.012.pos"
-  - impute_hap:
-      - meta:
+          ontologies: []
+  impute_hap:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.impute.hap":
           type: file
-          description: Phased haplotypes in IMPUTE reference-panel format (optional)
+          description: Phased haplotypes in IMPUTE reference-panel format
+            (optional)
           pattern: "*.impute.hap"
-  - impute_hap_legend:
-      - meta:
+          ontologies: []
+  impute_hap_legend:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -522,8 +584,9 @@ output:
           type: file
           description: Impute haplotype legend file (optional)
           pattern: "*.impute.hap.legend"
-  - impute_hap_indv:
-      - meta:
+          ontologies: []
+  impute_hap_indv:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -532,8 +595,9 @@ output:
           type: file
           description: Impute haplotype individuals file (optional)
           pattern: "*.impute.hap.indv"
-  - ldhat_sites:
-      - meta:
+          ontologies: []
+  ldhat_sites:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -542,8 +606,9 @@ output:
           type: file
           description: Output data in LDhat format, sites (optional)
           pattern: "*.ldhat.sites"
-  - ldhat_locs:
-      - meta:
+          ontologies: []
+  ldhat_locs:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -552,8 +617,9 @@ output:
           type: file
           description: output data in LDhat format, locations (optional)
           pattern: "*.ldhat.locs"
-  - beagle_gl:
-      - meta:
+          ontologies: []
+  beagle_gl:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -562,8 +628,9 @@ output:
           type: file
           description: Genotype likelihoods for biallelic sites (optional)
           pattern: "*.BEAGLE.GL"
-  - beagle_pl:
-      - meta:
+          ontologies: []
+  beagle_pl:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -572,8 +639,9 @@ output:
           type: file
           description: Genotype likelihoods for biallelic sites (optional)
           pattern: "*.BEAGLE.PL"
-  - ped:
-      - meta:
+          ontologies: []
+  ped:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -582,8 +650,9 @@ output:
           type: file
           description: output the genotype data in PLINK PED format (optional)
           pattern: "*.ped"
-  - map_:
-      - meta:
+          ontologies: []
+  map_:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -592,8 +661,9 @@ output:
           type: file
           description: output the genotype data in PLINK PED format (optional)
           pattern: "*.map"
-  - tped:
-      - meta:
+          ontologies: []
+  tped:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -602,8 +672,9 @@ output:
           type: file
           description: output the genotype data in PLINK PED format (optional)
           pattern: "*.tped"
-  - tfam:
-      - meta:
+          ontologies: []
+  tfam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -612,62 +683,69 @@ output:
           type: file
           description: output the genotype data in PLINK PED format (optional)
           pattern: "*.tfam"
-  - diff_sites_in_files:
-      - meta:
+          ontologies: []
+  diff_sites_in_files:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.diff.sites_in_files":
           type: file
-          description: Sites that are common / unique to each file specified in optional
-            inputs (optional)
+          description: Sites that are common / unique to each file specified in
+            optional inputs (optional)
           pattern: "*.diff.sites.in.files"
-  - diff_indv_in_files:
-      - meta:
+          ontologies: []
+  diff_indv_in_files:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.diff.indv_in_files":
           type: file
-          description: Individuals that are common / unique to each file specified in
-            optional inputs (optional)
+          description: Individuals that are common / unique to each file specified
+            in optional inputs (optional)
           pattern: "*.diff.indv.in.files"
-  - diff_sites:
-      - meta:
+          ontologies: []
+  diff_sites:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.diff.sites":
           type: file
-          description: Discordance on a site by site basis, specified in optional inputs
-            (optional)
+          description: Discordance on a site by site basis, specified in optional
+            inputs (optional)
           pattern: "*.diff.sites"
-  - diff_indv:
-      - meta:
+          ontologies: []
+  diff_indv:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.diff.indv":
           type: file
-          description: Discordance on a individual by individual basis, specified in optional
-            inputs (optional)
+          description: Discordance on a individual by individual basis, specified
+            in optional inputs (optional)
           pattern: "*.diff.indv"
-  - diff_discd_matrix:
-      - meta:
+          ontologies: []
+  diff_discd_matrix:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.diff.discordance.matrix":
           type: file
-          description: Discordance matrix between files specified in optional inputs (optional)
+          description: Discordance matrix between files specified in optional
+            inputs (optional)
           pattern: "*.diff.discordance.matrix"
-  - diff_switch_error:
-      - meta:
+          ontologies: []
+  diff_switch_error:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -676,11 +754,30 @@ output:
           type: file
           description: Switch errors found between sites (optional)
           pattern: "*.diff.switch"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_vcftools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - vcftools:
+          type: string
+          description: The name of the tool
+      - vcftools --version 2>&1 | sed "s/^.*VCFtools (//;s/).*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - vcftools:
+          type: string
+          description: The name of the tool
+      - vcftools --version 2>&1 | sed "s/^.*VCFtools (//;s/).*//":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@Mark-S-Hill"
 maintainers:
diff --git a/subworkflows/local/bam_variant_calling_freebayes/main.nf b/subworkflows/local/bam_variant_calling_freebayes/main.nf
index e2a8d4d452..9491697a8a 100644
--- a/subworkflows/local/bam_variant_calling_freebayes/main.nf
+++ b/subworkflows/local/bam_variant_calling_freebayes/main.nf
@@ -64,10 +64,8 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     // Index the filtered VCFs
     TABIX_VC_FREEBAYES_FILT(vcf_filtered)
 
-    versions = versions.mix(FREEBAYES.out.versions)
     versions = versions.mix(TABIX_VC_FREEBAYES.out.versions)
     versions = versions.mix(TABIX_VC_FREEBAYES_FILT.out.versions)
-    versions = versions.mix(VCFLIB_VCFFILTER.out.versions)
 
     emit:
     vcf_unfiltered = ch_vcf // channel: [ meta, vcf, tbi ]
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index d09184133a..2fe3ed51c6 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -204,7 +204,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
         vcf_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.diploid_sv_vcf
         tbi_manta = BAM_VARIANT_CALLING_GERMLINE_MANTA.out.diploid_sv_vcf_tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_GERMLINE_MANTA.out.versions)
     }
 
     // INDEXCOV, for WGS only
@@ -352,7 +351,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
         vcf_strelka = BAM_VARIANT_CALLING_SINGLE_STRELKA.out.vcf
         tbi_strelka = BAM_VARIANT_CALLING_SINGLE_STRELKA.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SINGLE_STRELKA.out.versions)
     }
 
     // TIDDIT
@@ -361,12 +359,12 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             cram,
             // Remap channel to match module/subworkflow
             fasta,
+            fasta_fai,
             bwa
         )
 
         vcf_tiddit = BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.vcf
         tbi_tiddit = BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.versions)
     }
 
     vcf_all = channel.empty().mix(
diff --git a/subworkflows/local/bam_variant_calling_germline_manta/main.nf b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
index 27289fb833..f81dd5c75e 100644
--- a/subworkflows/local/bam_variant_calling_germline_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_manta/main.nf
@@ -15,7 +15,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi] or [ [], []] if no intervals; intervals file contains all intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ combined ->
@@ -35,8 +34,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_MANTA {
     diploid_sv_vcf                 = MANTA_GERMLINE.out.diploid_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
     diploid_sv_vcf_tbi             = MANTA_GERMLINE.out.diploid_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
-    versions = versions.mix(MANTA_GERMLINE.out.versions)
-
     emit:
     candidate_small_indels_vcf
     candidate_small_indels_vcf_tbi
@@ -45,5 +42,4 @@ workflow BAM_VARIANT_CALLING_GERMLINE_MANTA {
     diploid_sv_vcf
     diploid_sv_vcf_tbi
 
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_single_strelka/main.nf b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
index 004a57ca2c..6b8dbc3b9f 100644
--- a/subworkflows/local/bam_variant_calling_single_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_strelka/main.nf
@@ -17,7 +17,6 @@ workflow BAM_VARIANT_CALLING_SINGLE_STRELKA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ] or [ [], [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -64,11 +63,8 @@ workflow BAM_VARIANT_CALLING_SINGLE_STRELKA {
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], tbi ] }
 
-    versions = versions.mix(STRELKA_SINGLE.out.versions)
-
     emit:
     vcf
     tbi
 
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
index cea076093f..001fabc257 100644
--- a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
@@ -11,12 +11,12 @@ workflow BAM_VARIANT_CALLING_SINGLE_TIDDIT {
     take:
     cram
     fasta
+    fasta_fai
     bwa
 
     main:
-    versions = channel.empty()
 
-    TIDDIT_SV(cram, fasta, bwa)
+    TIDDIT_SV(cram, fasta.combine(fasta_fai).map { fasta_meta, fasta_path, _fai_meta, fai_path -> [ fasta_meta, fasta_path, fai_path ] }, bwa)
 
     TABIX_BGZIP_TIDDIT_SV(TIDDIT_SV.out.vcf)
 
@@ -24,11 +24,8 @@ workflow BAM_VARIANT_CALLING_SINGLE_TIDDIT {
     vcf = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, gz, _tbi -> [meta + [variantcaller: 'tiddit'], gz] }
     tbi = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, _gz, tbi -> [meta + [variantcaller: 'tiddit'], tbi] }
 
-    versions = versions.mix(TIDDIT_SV.out.versions)
-
     emit:
     ploidy
     vcf
     tbi
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index b856eaac93..10a7b40b8f 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -165,7 +165,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
 
         vcf_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.diploid_sv_vcf.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.somatic_sv_vcf)
         tbi_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.diploid_sv_vcf_tbi.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.somatic_sv_vcf_tbi)
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.versions)
     }
 
 
@@ -200,7 +199,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
 
         vcf_strelka = BAM_VARIANT_CALLING_SOMATIC_STRELKA.out.vcf
         tbi_strelka = BAM_VARIANT_CALLING_SOMATIC_STRELKA.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_STRELKA.out.versions)
     }
 
     // MSISENSORPRO
@@ -279,12 +277,12 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             cram.map { meta, normal_cram, normal_crai, _tumor_cram, _tumor_crai -> [meta, normal_cram, normal_crai] },
             cram.map { meta, _normal_cram, _normal_crai, tumor_cram, tumor_crai -> [meta, tumor_cram, tumor_crai] },
             fasta,
+            fasta_fai,
             bwa,
         )
 
         vcf_tiddit = BAM_VARIANT_CALLING_SOMATIC_TIDDIT.out.vcf
         tbi_tiddit = BAM_VARIANT_CALLING_SOMATIC_TIDDIT.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_TIDDIT.out.versions)
     }
 
     vcf_all = channel.empty()
diff --git a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
index f1766192c8..ce4abac08d 100644
--- a/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_manta/main.nf
@@ -14,7 +14,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi ] or [ [], [] ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ combined ->
@@ -36,8 +35,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
     somatic_sv_vcf = MANTA_SOMATIC.out.somatic_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
     somatic_sv_vcf_tbi = MANTA_SOMATIC.out.somatic_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
-    versions = versions.mix(MANTA_SOMATIC.out.versions)
-
     emit:
     candidate_small_indels_vcf
     candidate_small_indels_vcf_tbi
@@ -48,5 +45,4 @@ workflow BAM_VARIANT_CALLING_SOMATIC_MANTA {
     somatic_sv_vcf
     somatic_sv_vcf_tbi
 
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
index ae73178155..001e021e47 100644
--- a/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
@@ -17,7 +17,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi, num_intervals ] or [ [], [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram.combine(intervals)
@@ -70,11 +69,8 @@ workflow BAM_VARIANT_CALLING_SOMATIC_STRELKA {
         // add variantcaller to meta map and remove no longer necessary field: num_intervals
         .map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'strelka' ], tbi ] }
 
-    versions = versions.mix(STRELKA_SOMATIC.out.versions)
-
     emit:
     vcf
     tbi
 
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf b/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
index 30eed94927..73d9cc9ef3 100644
--- a/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
@@ -13,26 +13,20 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TIDDIT {
         cram_normal
         cram_tumor
         fasta
+        fasta_fai
         bwa
 
     main:
 
-    versions = channel.empty()
-
-    TIDDIT_NORMAL(cram_normal, fasta, bwa)
-    TIDDIT_TUMOR(cram_tumor, fasta, bwa)
+    TIDDIT_NORMAL(cram_normal, fasta, fasta_fai, bwa)
+    TIDDIT_TUMOR(cram_tumor, fasta, fasta_fai, bwa)
 
     SVDB_MERGE(TIDDIT_NORMAL.out.vcf.join(TIDDIT_TUMOR.out.vcf, failOnDuplicate: true, failOnMismatch: true).map{ meta, vcf_normal, vcf_tumor -> [ meta, [vcf_normal, vcf_tumor] ] }, false, true)
 
     vcf = SVDB_MERGE.out.vcf.map{ meta, vcf -> [ meta + [ variantcaller:'tiddit' ], vcf ] }
     tbi = SVDB_MERGE.out.tbi.map{ meta, tbi -> [ meta + [ variantcaller:'tiddit' ], tbi ] }
 
-    versions = versions.mix(TIDDIT_NORMAL.out.versions)
-    versions = versions.mix(TIDDIT_TUMOR.out.versions)
-    versions = versions.mix(SVDB_MERGE.out.versions)
-
     emit:
-    versions
     vcf
     tbi
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 91afd8a217..12e2e570e5 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -167,7 +167,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
         )
         vcf_lofreq = BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ.out.vcf
         tbi_lofreq = BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ.out.versions)
     }
 
     // MANTA
@@ -181,7 +180,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
 
         vcf_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.tumor_sv_vcf
         tbi_manta = BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.tumor_sv_vcf_tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA.out.versions)
     }
 
     // TIDDIT
@@ -189,12 +187,12 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
         BAM_VARIANT_CALLING_SINGLE_TIDDIT(
             cram,
             fasta,
+            fasta_fai,
             bwa,
         )
 
         vcf_tiddit = BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.vcf
         tbi_tiddit = BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_SINGLE_TIDDIT.out.versions)
     }
 
     // TNSCOPE
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
index fd38480bbc..e8852db2e4 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_lofreq/main.nf
@@ -10,7 +10,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ {
     dict      // channel: /path/to/reference/fasta/dictionary
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
@@ -43,10 +42,7 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_LOFREQ {
     vcf = channel.empty().mix(MERGE_LOFREQ.out.vcf, vcf_branch.no_intervals).map{ meta, vcf -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], vcf ] }
     tbi = channel.empty().mix(MERGE_LOFREQ.out.tbi, tbi_branch.no_intervals).map{ meta, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'lofreq' ], tbi ] }
 
-    versions = versions.mix(LOFREQ.out.versions)
-
     emit:
     vcf
     tbi
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
index 380121ef9b..3e0c289604 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
@@ -15,7 +15,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA {
     intervals     // channel: [mandatory] [ interval.bed.gz, interval.bed.gz.tbi ] or [ [], [] ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals, account for 0 intervals
     cram_intervals = cram.combine(intervals).map{ combined ->
@@ -35,8 +34,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA {
     tumor_sv_vcf = MANTA_TUMORONLY.out.tumor_sv_vcf.map{ meta, vcf -> [ meta + [ variantcaller:'manta' ], vcf ] }
     tumor_sv_vcf_tbi = MANTA_TUMORONLY.out.tumor_sv_vcf_tbi.map{ meta, tbi -> [ meta + [ variantcaller:'manta' ], tbi ] }
 
-    versions = versions.mix(MANTA_TUMORONLY.out.versions)
-
     emit:
     candidate_small_indels_vcf
     candidate_small_indels_vcf_tbi
@@ -45,5 +42,4 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_MANTA {
     tumor_sv_vcf
     tumor_sv_vcf_tbi
 
-    versions
 }
diff --git a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
index 85aa418968..031802d638 100644
--- a/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
+++ b/subworkflows/local/vcf_qc_bcftools_vcftools/main.nf
@@ -10,20 +10,15 @@ workflow VCF_QC_BCFTOOLS_VCFTOOLS {
 
     main:
 
-    versions = channel.empty()
-
     BCFTOOLS_STATS(vcf.map{ meta, vcf_ -> [ meta, vcf_, [] ] }, [[:],[]], [[:],[]], [[:],[]], [[:],[]], [[:],[]])
     VCFTOOLS_TSTV_COUNT(vcf, target_bed, [])
     VCFTOOLS_TSTV_QUAL(vcf, target_bed, [])
     VCFTOOLS_SUMMARY(vcf, target_bed, [])
 
-    versions = versions.mix(VCFTOOLS_TSTV_COUNT.out.versions)
-
     emit:
     bcftools_stats          = BCFTOOLS_STATS.out.stats
     vcftools_tstv_counts    = VCFTOOLS_TSTV_COUNT.out.tstv_count
     vcftools_tstv_qual      = VCFTOOLS_TSTV_QUAL.out.tstv_qual
     vcftools_filter_summary = VCFTOOLS_SUMMARY.out.filter_summary
 
-    versions
 }
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index bcd4637927..5eba11e3e1 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -525,8 +525,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index f465be1784..7a68e41943 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -58,8 +58,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -422,8 +428,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index c578704c66..c2f891429b 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -52,8 +52,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -379,8 +385,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/joint_calling_haplotypecaller.nf.test.snap b/tests/joint_calling_haplotypecaller.nf.test.snap
index 964d5f4f53..dd4c356dfe 100644
--- a/tests/joint_calling_haplotypecaller.nf.test.snap
+++ b/tests/joint_calling_haplotypecaller.nf.test.snap
@@ -41,8 +41,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -287,8 +293,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 6721c744eb..2ecca9bc76 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -32,8 +32,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -266,8 +272,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_concatenation.nf.test.snap b/tests/postprocess_concatenation.nf.test.snap
index 0efeecd74f..9eb4b11103 100644
--- a/tests/postprocess_concatenation.nf.test.snap
+++ b/tests/postprocess_concatenation.nf.test.snap
@@ -57,8 +57,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_concatenation_normalization.nf.test.snap b/tests/postprocess_concatenation_normalization.nf.test.snap
index b37818373b..3c4a7ecd72 100644
--- a/tests/postprocess_concatenation_normalization.nf.test.snap
+++ b/tests/postprocess_concatenation_normalization.nf.test.snap
@@ -66,8 +66,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -394,8 +400,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_consensus.nf.test.snap b/tests/postprocess_consensus.nf.test.snap
index 03d1848ea3..4273d70f6d 100644
--- a/tests/postprocess_consensus.nf.test.snap
+++ b/tests/postprocess_consensus.nf.test.snap
@@ -67,8 +67,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -430,8 +436,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -759,8 +771,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_filtering.nf.test.snap b/tests/postprocess_filtering.nf.test.snap
index f31b3554e2..b985b4bbda 100644
--- a/tests/postprocess_filtering.nf.test.snap
+++ b/tests/postprocess_filtering.nf.test.snap
@@ -47,8 +47,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_normalization.nf.test.snap b/tests/postprocess_normalization.nf.test.snap
index 2184783e7e..6465eb8a6e 100644
--- a/tests/postprocess_normalization.nf.test.snap
+++ b/tests/postprocess_normalization.nf.test.snap
@@ -57,8 +57,14 @@
                 "VCFS_NORM_SORT": {
                     "bcftools": "1.23.1"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index 2cbbd34d14..30076c3c58 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -95,8 +95,14 @@
                 "VARLOCIRAPTOR_PREPROCESS": {
                     "varlociraptor": "8.9.5"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -400,8 +406,14 @@
                 "VARLOCIRAPTOR_PREPROCESS": {
                     "varlociraptor": "8.9.5"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -648,8 +660,14 @@
                 "VARLOCIRAPTOR_PREPROCESS": {
                     "varlociraptor": "8.9.5"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 6ecc236aa4..52c8d0fe9d 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -70,8 +70,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/start_from_preparerecalibration.nf.test.snap b/tests/start_from_preparerecalibration.nf.test.snap
index 45e514d232..cb9de0448b 100644
--- a/tests/start_from_preparerecalibration.nf.test.snap
+++ b/tests/start_from_preparerecalibration.nf.test.snap
@@ -170,8 +170,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -298,8 +304,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/start_from_recalibration.nf.test.snap b/tests/start_from_recalibration.nf.test.snap
index aa77cbc709..b5143c63bc 100644
--- a/tests/start_from_recalibration.nf.test.snap
+++ b/tests/start_from_recalibration.nf.test.snap
@@ -150,8 +150,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -278,8 +284,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 17b602b3c5..2e35492574 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -55,8 +55,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index 3c42b848d9..29202ec5b1 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -45,8 +45,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -341,8 +347,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index 4415537702..c24a31fb1f 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -87,8 +87,8 @@
                     "strelka": "2.9.10"
                 },
                 "SVDB_MERGE": {
-                    "bcftools": 1.21,
-                    "svdb": "2.8.2"
+                    "bcftools": "1.23",
+                    "svdb": "2.8.4"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -109,13 +109,19 @@
                     "tabix": 1.21
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -566,9 +572,9 @@
                 "test2_vs_test.strelka.somatic_indels.TsTv.count:md5,8dcfdbcaac118df1d5ad407dd2af699f",
                 "test2_vs_test.strelka.somatic_snvs.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "test2_vs_test.strelka.somatic_snvs.TsTv.count:md5,8dcfdbcaac118df1d5ad407dd2af699f",
-                "test.tiddit.FILTER.summary:md5,2cb5598e2a83870e162787c5025c9518",
+                "test.tiddit.FILTER.summary:md5,34b4490cb507e54bce2cdff5d5594508",
                 "test.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
-                "test2_vs_test.tiddit_sv_merge.FILTER.summary:md5,2cb5598e2a83870e162787c5025c9518",
+                "test2_vs_test.tiddit_sv_merge.FILTER.summary:md5,34b4490cb507e54bce2cdff5d5594508",
                 "test2_vs_test.tiddit_sv_merge.TsTv.count:md5,8dcfdbcaac118df1d5ad407dd2af699f",
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.target.bed:md5,ab3aafe8cc4cc3f1c40d527dfad64fda",
@@ -624,10 +630,10 @@
                 "test.strelka.variants.vcf.gz:md5,666f835fdaf4952a179cdedd40c9d565",
                 "test2_vs_test.strelka.somatic_indels.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test2_vs_test.strelka.somatic_snvs.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test.tiddit.vcf.gz:md5,c74645590b3d3c8d5f34e1e681ccd05b",
-                "test2_vs_test.tiddit.normal.vcf.gz:md5,c74645590b3d3c8d5f34e1e681ccd05b",
-                "test2_vs_test.tiddit.tumor.vcf.gz:md5,a4a162ddf9a49df61c62abbf704e2d19",
-                "test2_vs_test.tiddit_sv_merge.vcf.gz:md5,9105fb1263deef2521b302b38d77cad7"
+                "test.tiddit.vcf.gz:md5,73a3716cb5280de3f7e70b5eaf6dd1d",
+                "test2_vs_test.tiddit.normal.vcf.gz:md5,73a3716cb5280de3f7e70b5eaf6dd1d",
+                "test2_vs_test.tiddit.tumor.vcf.gz:md5,4dfb5e0e5aa2c961d887a1633167ba71",
+                "test2_vs_test.tiddit_sv_merge.vcf.gz:md5,720f6c1c5b88e02529cb62d7d3d0a543"
             ],
             [
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1",
@@ -737,13 +743,19 @@
                     "tabix": 1.21
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1089,7 +1101,7 @@
                 "test.freebayes.filtered.TsTv.count:md5,845f64e5bb4224af98f3a47294cd5483",
                 "test.strelka.variants.FILTER.summary:md5,dd87f507da7de20d5318841af312493b",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
-                "test.tiddit.FILTER.summary:md5,2cb5598e2a83870e162787c5025c9518",
+                "test.tiddit.FILTER.summary:md5,34b4490cb507e54bce2cdff5d5594508",
                 "test.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.target.bed:md5,ab3aafe8cc4cc3f1c40d527dfad64fda",
@@ -1122,7 +1134,7 @@
                 "test.freebayes.filtered.vcf.gz:md5,bf085c88aa26191a55fbd23bff6a498f",
                 "test.strelka.genome.vcf.gz:md5,16437a040679d88b7d84a9276f793d6c",
                 "test.strelka.variants.vcf.gz:md5,666f835fdaf4952a179cdedd40c9d565",
-                "test.tiddit.vcf.gz:md5,ac44cc2f44ebec84f5377e3274131876"
+                "test.tiddit.vcf.gz:md5,5e603e7e628756413a2056ea873e14e1"
             ],
             [
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
@@ -1231,13 +1243,19 @@
                     "tabix": 1.21
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1557,7 +1575,7 @@
                 "test2.freebayes.filtered.TsTv.count:md5,60b173b4a649483b651fcfedf1f5d790",
                 "test2.mutect2.filtered.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "test2.mutect2.filtered.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
-                "test2.tiddit.FILTER.summary:md5,cea83f893b7e8a5744bde7c54486013a",
+                "test2.tiddit.FILTER.summary:md5,d35d41bdd3566a05c48057b8ca3d0e64",
                 "test2.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "cnvkit.reference.antitarget-tmp.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "cnvkit.reference.target-tmp.bed:md5,14a7ba28453f8c8fc6ba5b044c517291",
@@ -1579,7 +1597,7 @@
                 "test2.freebayes.filtered.vcf.gz:md5,8461ef8ccf651775bde7c29d0e563474",
                 "test2.mutect2.filtered.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test2.mutect2.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "test2.tiddit.vcf.gz:md5,a4a162ddf9a49df61c62abbf704e2d19"
+                "test2.tiddit.vcf.gz:md5,4dfb5e0e5aa2c961d887a1633167ba71"
             ],
             [
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1",
diff --git a/tests/variant_calling_deepvariant.nf.test.snap b/tests/variant_calling_deepvariant.nf.test.snap
index 8e01a6b047..d713e12cbd 100644
--- a/tests/variant_calling_deepvariant.nf.test.snap
+++ b/tests/variant_calling_deepvariant.nf.test.snap
@@ -29,8 +29,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -214,8 +220,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -355,8 +367,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -499,8 +517,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 94c0efd52b..c8c585f4d9 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -41,8 +41,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -265,8 +271,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -683,8 +695,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1039,8 +1057,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1370,8 +1394,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1604,8 +1634,14 @@
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_haplotypecaller.nf.test.snap b/tests/variant_calling_haplotypecaller.nf.test.snap
index 3d2db0137f..7016f600b7 100644
--- a/tests/variant_calling_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_haplotypecaller.nf.test.snap
@@ -19,8 +19,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -206,8 +212,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -392,8 +404,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -588,8 +606,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_lofreq.nf.test.snap b/tests/variant_calling_lofreq.nf.test.snap
index 57807564f3..dae6d760ce 100644
--- a/tests/variant_calling_lofreq.nf.test.snap
+++ b/tests/variant_calling_lofreq.nf.test.snap
@@ -28,6 +28,12 @@
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
                     "bgzip": "1.21",
                     "tabix": "1.21"
+                },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -206,6 +212,12 @@
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
                     "tabix": "1.21"
+                },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_manta.nf.test.snap b/tests/variant_calling_manta.nf.test.snap
index b5b032807a..b24430a374 100644
--- a/tests/variant_calling_manta.nf.test.snap
+++ b/tests/variant_calling_manta.nf.test.snap
@@ -26,8 +26,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -172,8 +178,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -324,8 +336,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -516,8 +534,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -675,8 +699,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -906,8 +936,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -1088,8 +1124,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_mpileup.nf.test.snap b/tests/variant_calling_mpileup.nf.test.snap
index 9cf0aebfc4..d4536963aa 100644
--- a/tests/variant_calling_mpileup.nf.test.snap
+++ b/tests/variant_calling_mpileup.nf.test.snap
@@ -26,8 +26,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -202,8 +208,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -381,8 +393,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -567,8 +585,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index 7bb29f3633..cb8540dbbb 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -36,8 +36,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -210,8 +216,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index a6adc2992f..c905361d6e 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -25,8 +25,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -219,8 +225,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -426,8 +438,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -634,8 +652,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_sentieon_dnascope.nf.test.snap b/tests/variant_calling_sentieon_dnascope.nf.test.snap
index 4de48c9aaa..1440d01f1a 100644
--- a/tests/variant_calling_sentieon_dnascope.nf.test.snap
+++ b/tests/variant_calling_sentieon_dnascope.nf.test.snap
@@ -29,8 +29,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -218,8 +224,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -416,8 +428,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
index 9375b5cee3..c50574d646 100644
--- a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
@@ -50,8 +50,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -241,8 +247,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -433,8 +445,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -625,8 +643,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_sentieon_tnscope.nf.test.snap b/tests/variant_calling_sentieon_tnscope.nf.test.snap
index ee0421f02f..924500df0d 100644
--- a/tests/variant_calling_sentieon_tnscope.nf.test.snap
+++ b/tests/variant_calling_sentieon_tnscope.nf.test.snap
@@ -30,8 +30,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -117,8 +123,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -220,8 +232,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -318,8 +336,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_strelka.nf.test.snap b/tests/variant_calling_strelka.nf.test.snap
index 058077e09f..11ec9a4495 100644
--- a/tests/variant_calling_strelka.nf.test.snap
+++ b/tests/variant_calling_strelka.nf.test.snap
@@ -29,8 +29,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -251,8 +257,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -436,8 +448,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -677,8 +695,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -858,8 +882,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_strelka_bp.nf.test.snap b/tests/variant_calling_strelka_bp.nf.test.snap
index 043b22d8fe..a24394f377 100644
--- a/tests/variant_calling_strelka_bp.nf.test.snap
+++ b/tests/variant_calling_strelka_bp.nf.test.snap
@@ -28,8 +28,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -301,8 +307,14 @@
                     "bgzip": "1.21",
                     "tabix": "1.21"
                 },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
+                },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/tests/variant_calling_tiddit.nf.test.snap b/tests/variant_calling_tiddit.nf.test.snap
index 40c6991379..2b872c1619 100644
--- a/tests/variant_calling_tiddit.nf.test.snap
+++ b/tests/variant_calling_tiddit.nf.test.snap
@@ -28,10 +28,16 @@
                     "tabix": "1.21"
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
+                },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -186,10 +192,16 @@
                     "tabix": "1.21"
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
+                },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
@@ -332,8 +344,8 @@
                     "samtools": 1.21
                 },
                 "SVDB_MERGE": {
-                    "bcftools": 1.21,
-                    "svdb": "2.8.2"
+                    "bcftools": "1.23",
+                    "svdb": "2.8.4"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -348,10 +360,16 @@
                     "tabix": "1.21"
                 },
                 "TIDDIT_SV": {
-                    "tiddit": "3.6.1"
+                    "tiddit": "3.9.5"
+                },
+                "VCFTOOLS_SUMMARY": {
+                    "vcftools": "0.1.17"
                 },
                 "VCFTOOLS_TSTV_COUNT": {
-                    "vcftools": "0.1.16"
+                    "vcftools": "0.1.17"
+                },
+                "VCFTOOLS_TSTV_QUAL": {
+                    "vcftools": "0.1.17"
                 }
             },
             [
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 2ee53dc1be..eef91cfbc7 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -551,7 +551,6 @@ workflow SAREK {
         versions = versions.mix(BAM_VARIANT_CALLING_GERMLINE_ALL.out.versions)
         versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_ALL.out.versions)
         versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_ALL.out.versions)
-        versions = versions.mix(VCF_QC_BCFTOOLS_VCFTOOLS.out.versions)
         versions = versions.mix(POST_VARIANTCALLING.out.versions)
 
         // ANNOTATE

From ac5e751eb030e3eb467cf8f97bbb730668fe8b5f Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Mon, 27 Jul 2026 14:42:13 +0200
Subject: [PATCH 17/27] chore(modules): migrate samtools modules to versions
 topic channel (#2241)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates the nf-core `samtools/*` modules to the `versions` topic
channel. **Stacked on #2240** (base: `topic/variant-calling`).

### Changes
- Updates
`samtools/{bam2fq,collatefastq,convert,faidx,index,merge,mpileup,stats,view}`
to their topic-channel versions.
- Removes the corresponding `.out.versions` wiring.
- Reworks call sites for the new input signatures: `fasta`+`fai`
combined into one `[meta, fasta, fai]` tuple (faidx/convert/merge/view),
`index_files`/`gzi` added to merge inputs, `view` given fai +
qname-tuple + bed-tuple.
- Tool bumps: samtools 1.21 → 1.24, htslib 1.21 → 1.24 (for the samtools
modules).

Test snapshots need regenerating in CI.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 .github/actions/get-shards/action.yml         |  10 +-
 .github/actions/nf-test/action.yml            |  46 +++++-
 .github/workflows/nf-test-gpu.yml             |   7 +
 .github/workflows/nf-test-sentieon.yml        |   7 +
 .github/workflows/nf-test.yml                 |   7 +
 CHANGELOG.md                                  |   4 +
 modules.json                                  |  20 +--
 modules/nf-core/mosdepth/environment.yml      |   6 +-
 modules/nf-core/mosdepth/main.nf              |  42 +++---
 modules/nf-core/mosdepth/meta.yml             | 126 +++++++++++-----
 .../nf-core/samtools/bam2fq/environment.yml   |   7 +-
 modules/nf-core/samtools/bam2fq/main.nf       |  61 +++++---
 modules/nf-core/samtools/bam2fq/meta.yml      |  72 +++++++---
 .../samtools/collatefastq/environment.yml     |   7 +-
 modules/nf-core/samtools/collatefastq/main.nf |  73 +++++-----
 .../nf-core/samtools/collatefastq/meta.yml    | 108 ++++++++------
 .../nf-core/samtools/convert/environment.yml  |   6 +-
 modules/nf-core/samtools/convert/main.nf      |  37 ++---
 modules/nf-core/samtools/convert/meta.yml     |  55 ++++---
 .../nf-core/samtools/faidx/environment.yml    |   6 +-
 modules/nf-core/samtools/faidx/main.nf        |  36 ++---
 modules/nf-core/samtools/faidx/meta.yml       |  39 +++--
 .../nf-core/samtools/index/environment.yml    |   6 +-
 modules/nf-core/samtools/index/main.nf        |  35 ++---
 modules/nf-core/samtools/index/meta.yml       |  61 ++++----
 .../nf-core/samtools/merge/environment.yml    |   6 +-
 modules/nf-core/samtools/merge/main.nf        |  48 +++----
 modules/nf-core/samtools/merge/meta.yml       |  82 ++++++-----
 .../nf-core/samtools/mpileup/environment.yml  |   7 +-
 modules/nf-core/samtools/mpileup/main.nf      |  43 +++---
 modules/nf-core/samtools/mpileup/meta.yml     |  60 ++++++--
 .../nf-core/samtools/stats/environment.yml    |   6 +-
 modules/nf-core/samtools/stats/main.nf        |  24 ++--
 modules/nf-core/samtools/stats/meta.yml       |  44 ++++--
 modules/nf-core/samtools/view/environment.yml |   5 +-
 modules/nf-core/samtools/view/main.nf         | 106 +++++++-------
 modules/nf-core/samtools/view/meta.yml        | 110 ++++++++++----
 subworkflows/local/bam_applybqsr/main.nf      |   6 -
 .../local/bam_applybqsr_spark/main.nf         |   6 -
 .../local/bam_convert_samtools/main.nf        |  32 ++---
 subworkflows/local/bam_markduplicates/main.nf |   2 +-
 .../local/bam_markduplicates_spark/main.nf    |   5 +-
 .../local/bam_merge_index_samtools/main.nf    |  10 +-
 subworkflows/local/bam_sentieon_dedup/main.nf |   2 +-
 .../bam_variant_calling_germline_all/main.nf  |   2 -
 .../local/bam_variant_calling_mpileup/main.nf |   9 +-
 .../bam_variant_calling_somatic_all/main.nf   |   4 -
 .../main.nf                                   |   1 -
 .../local/cram_merge_index_samtools/main.nf   |  10 +-
 .../local/cram_qc_mosdepth_samtools/main.nf   |  11 +-
 subworkflows/local/cram_sampleqc/main.nf      |   1 +
 .../fastq_create_umi_consensus_fgbio/main.nf  |   7 +-
 .../local/fastq_preprocess_gatk/main.nf       |  17 +--
 .../local/fastq_preprocess_parabricks/main.nf |   7 +-
 subworkflows/local/prepare_genome/main.nf     |   3 +-
 tests/aligner-bwa-mem.nf.test.snap            |  12 +-
 tests/aligner-bwa-mem2.nf.test.snap           |  12 +-
 tests/aligner-dragmap.nf.test.snap            |  12 +-
 tests/aligner-parabricks.nf.test.snap         |  96 +++++++------
 tests/alignment_from_everything.nf.test.snap  |  41 +++---
 tests/alignment_to_fastq.nf.test.snap         |  35 ++---
 tests/bbsplit.nf.test.snap                    |  28 ++--
 tests/default.nf.test.snap                    |  21 +--
 tests/fastp.nf.test.snap                      |  63 ++++----
 tests/intervals.nf.test.snap                  |  36 ++---
 ...joint_calling_haplotypecaller.nf.test.snap |  46 +++---
 tests/joint_calling_mutect2.nf.test.snap      |  50 +++----
 tests/lane_integer.nf.test.snap               |   6 +-
 tests/multi_lane.nf.test.snap                 |  39 ++---
 tests/postprocess_concatenation.nf.test.snap  |  19 +--
 ...s_concatenation_normalization.nf.test.snap |  38 ++---
 tests/postprocess_consensus.nf.test.snap      |  65 +++++----
 tests/postprocess_filtering.nf.test.snap      |  19 +--
 tests/postprocess_normalization.nf.test.snap  |  19 +--
 tests/postprocess_varlociraptor.nf.test.snap  |  55 +++----
 tests/qc_ngscheckmate.nf.test.snap            |  23 +--
 tests/save_mapped.nf.test.snap                |   6 +-
 tests/save_output_as_bam.nf.test.snap         |  25 ++--
 tests/sentieon.nf.test.snap                   |   4 +-
 tests/sentieon_aligner_bwamem.nf.test.snap    |   6 +-
 tests/sentieon_dedup.nf.test.snap             | 105 +++++++-------
 tests/spark.nf.test.snap                      |  27 ++--
 tests/start_from_markduplicates.nf.test.snap  |  84 +++++------
 ...art_from_preparerecalibration.nf.test.snap |  38 ++---
 tests/start_from_recalibration.nf.test.snap   |  38 ++---
 tests/tumor-normal-pair.nf.test.snap          |  21 +--
 tests/umi_fastp.nf.test.snap                  |  21 +--
 tests/umi_fgbio.nf.test.snap                  |  38 ++---
 tests/umi_in_read_names.nf.test.snap          |  56 ++++----
 tests/variant_calling_all.nf.test.snap        |  67 ++++-----
 tests/variant_calling_ascat.nf.test.snap      |  38 ++---
 tests/variant_calling_cnvkit.nf.test.snap     | 112 ++++++++-------
 .../variant_calling_controlfreec.nf.test.snap |  44 +++---
 .../variant_calling_deepvariant.nf.test.snap  |  34 ++---
 tests/variant_calling_freebayes.nf.test.snap  | 136 +++++++++---------
 ...riant_calling_haplotypecaller.nf.test.snap |  72 +++++-----
 tests/variant_calling_lofreq.nf.test.snap     |  28 ++--
 tests/variant_calling_manta.nf.test.snap      | 101 +++++++------
 tests/variant_calling_mpileup.nf.test.snap    |  68 ++++-----
 tests/variant_calling_msisensor2.nf.test.snap |  19 +--
 .../variant_calling_msisensorpro.nf.test.snap |  23 +--
 tests/variant_calling_muse.nf.test.snap       |  25 ++--
 tests/variant_calling_mutect2.nf.test.snap    |  78 +++++-----
 ...ant_calling_sentieon_dnascope.nf.test.snap |  51 +++----
 ...ling_sentieon_haplotypecaller.nf.test.snap |  68 ++++-----
 tests/variant_calling_strelka.nf.test.snap    |  95 ++++++------
 tests/variant_calling_strelka_bp.nf.test.snap |  38 ++---
 tests/variant_calling_tiddit.nf.test.snap     |  41 +++---
 workflows/sarek.nf                            |  12 +-
 109 files changed, 2147 insertions(+), 1767 deletions(-)

diff --git a/.github/actions/get-shards/action.yml b/.github/actions/get-shards/action.yml
index e2833ee979..671ac1e101 100644
--- a/.github/actions/get-shards/action.yml
+++ b/.github/actions/get-shards/action.yml
@@ -10,6 +10,10 @@ inputs:
   tags:
     description: "Tags to pass as argument for nf-test --tag parameter"
     required: false
+  all_tests:
+    description: "Count all tagged tests instead of only those changed since HEAD^ (used for snapshot regeneration)"
+    required: false
+    default: "false"
 outputs:
   shard:
     description: "Array of shard numbers"
@@ -29,12 +33,16 @@ runs:
       shell: bash
       run: |
         # Run nf-test with dynamic parameter
+        # In all_tests mode (snapshot regeneration) count every tagged test,
+        # not just those changed since HEAD^.
+        CHANGED_SINCE="--changed-since HEAD^"
+        if [ "${{ inputs.all_tests }}" = "true" ]; then CHANGED_SINCE=""; fi
         nftest_output=$(nf-test test \
           --profile +docker \
           $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
           --dry-run \
           --ci \
-          --changed-since HEAD^) || {
+          $CHANGED_SINCE) || {
             echo "nf-test command failed with exit code $?"
             echo "Full output: $nftest_output"
             exit 1
diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
index 0ad890dc3e..341139f2f3 100644
--- a/.github/actions/nf-test/action.yml
+++ b/.github/actions/nf-test/action.yml
@@ -16,6 +16,10 @@ inputs:
   tags:
     description: "Tags to pass as argument for nf-test --tag parameter"
     required: false
+  update_snapshots:
+    description: "Run with --update-snapshot over all tagged tests and upload regenerated snapshots as artifacts"
+    required: false
+    default: "false"
 runs:
   using: "composite"
   steps:
@@ -70,12 +74,28 @@ runs:
         NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
         SENTIEON_LICSRVR_IP: ${{ env.SENTIEON_LICSRVR_IP }}
         SENTIEON_AUTH_MECH: "GitHub Actions - token"
+        # Use nf-test's native differ instead of pdiff: pdiff wraps the snapshot
+        # diff to the terminal width and truncates md5 checksums in the CI log.
+        # The native differ prints full-length values on one line (scrapeable).
+        NFT_DIFF: diff
       run: |
+        # setup-nf-test exports NFT_DIFF_ARGS (pdiff flags) into the env; those
+        # flags suppress the native differ's output entirely, so drop them here.
+        unset NFT_DIFF_ARGS
+        # In update-snapshot mode, regenerate snapshots over ALL tagged tests
+        # (drop --changed-since, which is only meaningful for PR/push diffs).
+        CHANGED_SINCE="--changed-since HEAD^"
+        UPDATE_SNAPSHOT=""
+        if [ "${{ inputs.update_snapshots }}" = "true" ]; then
+          CHANGED_SINCE=""
+          UPDATE_SNAPSHOT="--update-snapshot"
+        fi
         nf-test test \
           --profile=+${{ inputs.profile }} \
           $(if [ -n "${{ inputs.tags }}" ]; then echo "--tag ${{ inputs.tags }}"; fi) \
           --ci \
-          --changed-since HEAD^ \
+          $CHANGED_SINCE \
+          $UPDATE_SNAPSHOT \
           --verbose \
           --tap=test.tap \
           --shard ${{ inputs.shard }}/${{ inputs.total_shards }}
@@ -83,6 +103,30 @@ runs:
           # Save the absolute path of the test.tap file to the output
           echo "tap_file_path=$(realpath test.tap)" >> $GITHUB_OUTPUT
 
+    # ---- update-snapshot mode: collect & upload regenerated snapshots ----
+    # Only the canonical docker + pinned-Nextflow leg uploads, to avoid
+    # profile/version-specific artifact collisions.
+    - name: Collect updated snapshots
+      if: ${{ inputs.update_snapshots == 'true' && inputs.profile == 'docker' && env.NXF_VERSION == '25.10.4' }}
+      shell: bash
+      run: |
+        rm -rf updated-snaps && mkdir -p updated-snaps
+        git diff --name-only -- 'tests/*.snap' | while read -r f; do
+          mkdir -p "updated-snaps/$(dirname "$f")"
+          cp "$f" "updated-snaps/$f"
+        done
+        echo "Changed snapshot files (shard ${{ inputs.shard }}/${{ inputs.total_shards }}):"
+        git diff --name-only -- 'tests/*.snap' || true
+
+    - name: Upload updated snapshots
+      if: ${{ inputs.update_snapshots == 'true' && inputs.profile == 'docker' && env.NXF_VERSION == '25.10.4' }}
+      uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+      with:
+        name: updated-snapshots-shard-${{ inputs.shard }}
+        path: updated-snaps/
+        if-no-files-found: ignore
+        retention-days: 5
+
     - name: Generate test summary
       if: always()
       shell: bash
diff --git a/.github/workflows/nf-test-gpu.yml b/.github/workflows/nf-test-gpu.yml
index e6ee69ddea..07734a673b 100644
--- a/.github/workflows/nf-test-gpu.yml
+++ b/.github/workflows/nf-test-gpu.yml
@@ -10,6 +10,11 @@ on:
   release:
     types: [published]
   workflow_dispatch:
+    inputs:
+      update_snapshots:
+        description: "Regenerate snapshots (--update-snapshot) over all tagged tests and upload them as artifacts"
+        type: boolean
+        default: false
 
 # Cancel if a newer run is started
 concurrency:
@@ -51,6 +56,7 @@ jobs:
         with:
           tags: "gpu"
           max_shards: 2
+          all_tests: ${{ github.event.inputs.update_snapshots || 'false' }}
 
       - name: debug
         run: |
@@ -108,6 +114,7 @@ jobs:
           shard: ${{ matrix.shard }}
           total_shards: ${{ env.TOTAL_SHARDS }}
           tags: ${{ matrix.profile == 'conda' && 'gpu_conda' || 'gpu,gpu_conda' }}
+          update_snapshots: ${{ github.event.inputs.update_snapshots || 'false' }}
       - name: Report test status
         if: ${{ always() }}
         run: |
diff --git a/.github/workflows/nf-test-sentieon.yml b/.github/workflows/nf-test-sentieon.yml
index 6b7d5de27c..cdf6f5530c 100644
--- a/.github/workflows/nf-test-sentieon.yml
+++ b/.github/workflows/nf-test-sentieon.yml
@@ -10,6 +10,11 @@ on:
   release:
     types: [published]
   workflow_dispatch:
+    inputs:
+      update_snapshots:
+        description: "Regenerate snapshots (--update-snapshot) over all tagged tests and upload them as artifacts"
+        type: boolean
+        default: false
 
 # Cancel if a newer run is started
 concurrency:
@@ -51,6 +56,7 @@ jobs:
         with:
           tags: "sentieon,sentieon_conda"
           max_shards: 10
+          all_tests: ${{ github.event.inputs.update_snapshots || 'false' }}
 
       - name: debug
         run: |
@@ -109,6 +115,7 @@ jobs:
           shard: ${{ matrix.shard }}
           total_shards: ${{ env.TOTAL_SHARDS }}
           tags: ${{ matrix.profile == 'conda' && 'sentieon_conda' || 'sentieon,sentieon_conda' }}
+          update_snapshots: ${{ github.event.inputs.update_snapshots || 'false' }}
       - name: Report test status
         if: ${{ always() }}
         run: |
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index 88ddc03266..29987b5c7a 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -10,6 +10,11 @@ on:
   release:
     types: [published]
   workflow_dispatch:
+    inputs:
+      update_snapshots:
+        description: "Regenerate snapshots (--update-snapshot) over all tagged tests and upload them as artifacts"
+        type: boolean
+        default: false
 
 # Cancel if a newer run is started
 concurrency:
@@ -52,6 +57,7 @@ jobs:
         with:
           max_shards: 15
           tags: "cpu,cpu_conda"
+          all_tests: ${{ github.event.inputs.update_snapshots || 'false' }}
 
       - name: debug
         run: |
@@ -103,6 +109,7 @@ jobs:
           shard: ${{ matrix.shard }}
           total_shards: ${{ env.TOTAL_SHARDS }}
           tags: ${{ matrix.profile == 'conda' && 'cpu_conda' || 'cpu,cpu_conda' }}
+          update_snapshots: ${{ github.event.inputs.update_snapshots || 'false' }}
 
       - name: Report test status
         if: ${{ always() }}
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3e28d64d3f..cbac014e25 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -21,11 +21,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2238](https://github.com/nf-core/sarek/pull/2238) - Migrate `gatk4`/`gatk4spark` modules to the versions topic channel (bumps gatk4spark 4.6.1.0 → 4.6.2.0)
 - [#2239](https://github.com/nf-core/sarek/pull/2239) - Migrate alignment/UMI/utility modules (`bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`, `gunzip`, `untar`, `unzip`, `spring`) to the versions topic channel (fastp 0.24.0 → 1.1.0)
 - [#2240](https://github.com/nf-core/sarek/pull/2240) - Migrate variant-calling modules (`freebayes`, `strelka`, `manta`, `tiddit`, `lofreq`, `svdb`, `vcflib`, `vcftools`) to the versions topic channel
+- [#2241](https://github.com/nf-core/sarek/pull/2241) - Migrate `samtools/*` and `mosdepth` modules to the versions topic channel
 
 ### Fixed
 
 - [#2184](https://github.com/nf-core/sarek/pull/2184) - Skip nf-schema path-existence validation for `snpeff_cache`, `vep_cache` and `igenomes_base` so pipeline launches succeed when the default S3 buckets are not accessible
 - [#2216](https://github.com/nf-core/sarek/pull/2216) - Fix `--normalize_vcfs` dropping a real ALT allele of `1/2` multiallelic sites (`bcftools norm --rm-dup all` → `--rm-dup exact`)
+- [#2241](https://github.com/nf-core/sarek/pull/2241) - Bump `mosdepth` (0.3.10 → 0.3.14) so its htslib can decode CRAM 3.1 written by samtools 1.24; the previous container silently produced empty coverage files, dropping the mosdepth MultiQC sections (most visible in the parabricks path)
 
 ### Removed
 
@@ -48,6 +50,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | svdb          | 2.8.2       | 2.8.4       |
 | tiddit        | 3.6.1       | 3.9.5       |
 | vcftools      | 0.1.16      | 0.1.17      |
+| samtools      | 1.21        | 1.24        |
+| mosdepth      | 0.3.10      | 0.3.14      |
 
 ### Dependencies - plugins
 
diff --git a/modules.json b/modules.json
index e883cb77a7..fc37c5232f 100644
--- a/modules.json
+++ b/modules.json
@@ -368,7 +368,7 @@
                     },
                     "mosdepth": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "999b9db1d6b8bdc591e1168e183f4cc87d6606f9",
                         "installed_by": ["modules"]
                     },
                     "msisensor2/msi": {
@@ -418,47 +418,47 @@
                     },
                     "samtools/bam2fq": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/collatefastq": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/convert": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/faidx": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/index": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/merge": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/mpileup": {
                         "branch": "master",
-                        "git_sha": "7e20d971c70d78dbd9f610698267f37b7fb3d38a",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/stats": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "samtools/view": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "9339809fcb90af8a8b7051e6cd914894d5c52002",
                         "installed_by": ["modules"]
                     },
                     "sentieon/applyvarcal": {
diff --git a/modules/nf-core/mosdepth/environment.yml b/modules/nf-core/mosdepth/environment.yml
index f871e054e4..1462ab3409 100644
--- a/modules/nf-core/mosdepth/environment.yml
+++ b/modules/nf-core/mosdepth/environment.yml
@@ -4,5 +4,9 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.23.1
   # renovate: datasource=conda depName=bioconda/mosdepth
-  - mosdepth=0.3.10
+  - bioconda::mosdepth=0.3.14
+  # renovate: datasource=conda depName=conda-forge/gzip
+  - conda-forge::gzip=1.14
diff --git a/modules/nf-core/mosdepth/main.nf b/modules/nf-core/mosdepth/main.nf
index 3bf945f909..ef82be0a17 100644
--- a/modules/nf-core/mosdepth/main.nf
+++ b/modules/nf-core/mosdepth/main.nf
@@ -3,13 +3,14 @@ process MOSDEPTH {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mosdepth:0.3.10--h4e814b3_1' :
-        'biocontainers/mosdepth:0.3.10--h4e814b3_1'}"
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
+        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/a3/a3dc5ea2ce788c24079d24d1721ed28086874152c43b5e7dde3f638dcf64336a/data' :
+        'community.wave.seqera.io/library/htslib_mosdepth_gzip:4108dd38be84e40a'}"
 
     input:
     tuple val(meta),  path(bam), path(bai), path(bed)
     tuple val(meta2), path(fasta)
+    val(quantize_labels)
 
     output:
     tuple val(meta), path('*.global.dist.txt')      , emit: global_txt
@@ -24,8 +25,8 @@ process MOSDEPTH {
     tuple val(meta), path('*.quantized.bed.gz.csi') , optional:true, emit: quantized_csi
     tuple val(meta), path('*.thresholds.bed.gz')    , optional:true, emit: thresholds_bed
     tuple val(meta), path('*.thresholds.bed.gz.csi'), optional:true, emit: thresholds_csi
-    path  "versions.yml"                            , emit: versions
-
+    tuple val("${task.process}"), val('mosdepth'), eval("mosdepth --version | sed 's/mosdepth //g'"), topic: versions, emit: versions_mosdepth
+    tuple val("${task.process}"), val('gzip'), eval("gzip -V 2>&1 | sed 's/gzip \\([0-9.]*\\).*/\\1/;q'"), topic: versions, emit: versions_gzip
     when:
     task.ext.when == null || task.ext.when
 
@@ -34,30 +35,38 @@ process MOSDEPTH {
     def prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--fasta ${fasta}" : ""
     def interval = bed ? "--by ${bed}" : ""
-    if (bed && args.contains("--by")) {
+    def quantize_env_vars = []
+    if (quantize_labels instanceof List && quantize_labels.size() > 0) {
+        quantize_labels.eachWithIndex { label, index ->
+            quantize_env_vars << "MOSDEPTH_Q${index}=${label}"
+        }
+    }
+    if (bed && (args.contains("--by") || args.contains("-b "))) {
         error "'--by' can only be specified once when running mosdepth! Either remove input BED file definition or remove '--by' from 'ext.args' definition"
     }
-    if (!bed && args.contains("--thresholds")) {
-        error "'--thresholds' can only be specified in conjunction with '--by'"
+    if (args.contains("--thresholds") && !(bed || args.contains("--by") || args.contains("-b "))) {
+        error "'--thresholds' can only be specified in conjunction with '--by' or an input bed file"
     }
 
     """
-    mosdepth \\
+    ${quantize_env_vars.join(" ")} mosdepth \\
         --threads $task.cpus \\
         $interval \\
         $reference \\
         $args \\
         $prefix \\
         $bam
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        mosdepth: \$(mosdepth --version 2>&1 | sed 's/^.*mosdepth //; s/ .*\$//')
-    END_VERSIONS
     """
 
     stub:
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
+    if (bed && (args.contains("--by") || args.contains("-b "))) {
+        error "'--by' can only be specified once when running mosdepth! Either remove input BED file definition or remove '--by' from 'ext.args' definition"
+    }
+    if (args.contains("--thresholds") && !(bed || args.contains("--by") || args.contains("-b "))) {
+        error "'--thresholds' can only be specified in conjunction with '--by' or an input bed file"
+    }
     """
     touch ${prefix}.global.dist.txt
     touch ${prefix}.region.dist.txt
@@ -71,10 +80,5 @@ process MOSDEPTH {
     touch ${prefix}.quantized.bed.gz.csi
     echo "" | gzip > ${prefix}.thresholds.bed.gz
     touch ${prefix}.thresholds.bed.gz.csi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        mosdepth: \$(mosdepth --version 2>&1 | sed 's/^.*mosdepth //; s/ .*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/mosdepth/meta.yml b/modules/nf-core/mosdepth/meta.yml
index dc783c9006..36097fca8e 100644
--- a/modules/nf-core/mosdepth/meta.yml
+++ b/modules/nf-core/mosdepth/meta.yml
@@ -11,7 +11,8 @@ tools:
         Fast BAM/CRAM depth calculation for WGS, exome, or targeted sequencing.
       documentation: https://github.com/brentp/mosdepth
       doi: 10.1093/bioinformatics/btx699
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:mosdepth
 input:
   - - meta:
@@ -23,14 +24,17 @@ input:
         type: file
         description: Input BAM/CRAM file
         pattern: "*.{bam,cram}"
+        ontologies: []
     - bai:
         type: file
         description: Index for BAM/CRAM file
         pattern: "*.{bai,crai}"
+        ontologies: []
     - bed:
         type: file
         description: BED file with intersected intervals
         pattern: "*.{bed}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -40,9 +44,16 @@ input:
         type: file
         description: Reference genome FASTA file
         pattern: "*.{fa,fasta}"
+        ontologies: []
+  - quantize_labels:
+      type: list
+      description: |
+        List of labels for quantized coverage bins, e.g. [ "NO_COVERAGE", "LOW_COVERAGE", "MEDIUM_COVERAGE", "HIGH_COVERAGE" ]
+        The first value will be assigned to the `MOSDEPTH_Q0` environment variable, the second to `MOSDEPTH_Q1`, and so on.
+        These can then be used in the `--quantize` option of mosdepth to assign labels to quantized coverage bins.
 output:
-  - global_txt:
-      - meta:
+  global_txt:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,18 +62,21 @@ output:
           type: file
           description: Text file with global cumulative coverage distribution
           pattern: "*.{global.dist.txt}"
-  - summary_txt:
-      - meta:
+          ontologies: []
+  summary_txt:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.summary.txt":
           type: file
-          description: Text file with summary mean depths per chromosome and regions
+          description: Text file with summary mean depths per chromosome and
+            regions
           pattern: "*.{summary.txt}"
-  - regions_txt:
-      - meta:
+          ontologies: []
+  regions_txt:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,8 +85,9 @@ output:
           type: file
           description: Text file with region cumulative coverage distribution
           pattern: "*.{region.dist.txt}"
-  - per_base_d4:
-      - meta:
+          ontologies: []
+  per_base_d4:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,8 +96,9 @@ output:
           type: file
           description: D4 file with per-base coverage
           pattern: "*.{per-base.d4}"
-  - per_base_bed:
-      - meta:
+          ontologies: []
+  per_base_bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,8 +107,9 @@ output:
           type: file
           description: BED file with per-base coverage
           pattern: "*.{per-base.bed.gz}"
-  - per_base_csi:
-      - meta:
+          ontologies: []
+  per_base_csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -101,8 +118,9 @@ output:
           type: file
           description: Index file for BED file with per-base coverage
           pattern: "*.{per-base.bed.gz.csi}"
-  - regions_bed:
-      - meta:
+          ontologies: []
+  regions_bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -111,8 +129,9 @@ output:
           type: file
           description: BED file with per-region coverage
           pattern: "*.{regions.bed.gz}"
-  - regions_csi:
-      - meta:
+          ontologies: []
+  regions_csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -121,8 +140,9 @@ output:
           type: file
           description: Index file for BED file with per-region coverage
           pattern: "*.{regions.bed.gz.csi}"
-  - quantized_bed:
-      - meta:
+          ontologies: []
+  quantized_bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -131,8 +151,9 @@ output:
           type: file
           description: BED file with binned coverage
           pattern: "*.{quantized.bed.gz}"
-  - quantized_csi:
-      - meta:
+          ontologies: []
+  quantized_csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -141,19 +162,21 @@ output:
           type: file
           description: Index file for BED file with binned coverage
           pattern: "*.{quantized.bed.gz.csi}"
-  - thresholds_bed:
-      - meta:
+          ontologies: []
+  thresholds_bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.thresholds.bed.gz":
           type: file
-          description: BED file with the number of bases in each region that are covered
-            at or above each threshold
+          description: BED file with the number of bases in each region that are
+            covered at or above each threshold
           pattern: "*.{thresholds.bed.gz}"
-  - thresholds_csi:
-      - meta:
+          ontologies: []
+  thresholds_csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -162,11 +185,47 @@ output:
           type: file
           description: Index file for BED file with threshold coverage
           pattern: "*.{thresholds.bed.gz.csi}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_mosdepth:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - mosdepth:
+          type: string
+          description: The tool name
+      - mosdepth --version | sed 's/mosdepth //g':
+          type: eval
+          description: The command used to generate the version of the tool
+  versions_gzip:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - gzip:
+          type: string
+          description: The name of the tool
+      - gzip -V 2>&1 | sed 's/gzip \([0-9.]*\).*/\1/;q':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - mosdepth:
+          type: string
+          description: The tool name
+      - mosdepth --version | sed 's/mosdepth //g':
+          type: eval
+          description: The command used to generate the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - gzip:
+          type: string
+          description: The name of the tool
+      - gzip -V 2>&1 | sed 's/gzip \([0-9.]*\).*/\1/;q':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@joseespinosa"
   - "@drpatelh"
@@ -174,6 +233,5 @@ authors:
   - "@matthdsm"
 maintainers:
   - "@joseespinosa"
-  - "@drpatelh"
   - "@ramprasadn"
   - "@matthdsm"
diff --git a/modules/nf-core/samtools/bam2fq/environment.yml b/modules/nf-core/samtools/bam2fq/environment.yml
index 62054fc97a..b08c7761d8 100644
--- a/modules/nf-core/samtools/bam2fq/environment.yml
+++ b/modules/nf-core/samtools/bam2fq/environment.yml
@@ -4,5 +4,8 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
+  - conda-forge::xz=5.8.3
diff --git a/modules/nf-core/samtools/bam2fq/main.nf b/modules/nf-core/samtools/bam2fq/main.nf
index 1d3049e565..d5fde581ac 100644
--- a/modules/nf-core/samtools/bam2fq/main.nf
+++ b/modules/nf-core/samtools/bam2fq/main.nf
@@ -1,11 +1,11 @@
 process SAMTOOLS_BAM2FQ {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/31/315d2445cd42b0f5512fa37965a9c59bc93ae8614b7d105150caece6c61e2e71/data'
+        : 'community.wave.seqera.io/library/htslib_samtools_xz:1595ae0727655963'}"
 
     input:
     tuple val(meta), path(inputbam)
@@ -13,7 +13,8 @@ process SAMTOOLS_BAM2FQ {
 
     output:
     tuple val(meta), path("*.fq.gz"), emit: reads
-    path "versions.yml"             , emit: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
+    tuple val("${task.process}"), val("bgzip"), eval('bgzip --version | head -1 | sed "s/bgzip (htslib) //"'), emit: versions_bgzip, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -22,35 +23,49 @@ process SAMTOOLS_BAM2FQ {
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
 
-    if (split){
+    if (split) {
         """
         samtools \\
             bam2fq \\
-            $args \\
-            -@ $task.cpus \\
+            ${args} \\
+            -@ ${task.cpus} \\
             -1 ${prefix}_1.fq.gz \\
             -2 ${prefix}_2.fq.gz \\
             -0 ${prefix}_other.fq.gz \\
             -s ${prefix}_singleton.fq.gz \\
-            $inputbam
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
+            ${inputbam}
         """
-    } else {
+    }
+    else {
         """
         samtools \\
             bam2fq \\
-            $args \\
-            -@ $task.cpus \\
-            $inputbam | gzip --no-name > ${prefix}_interleaved.fq.gz
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-        END_VERSIONS
+            ${args} \\
+            -@ ${task.cpus} \\
+            ${inputbam} | bgzip > ${prefix}_interleaved.fq.gz
+        """
+    }
+
+    stub:
+
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def bgzip_command_1 = "echo | bgzip -c > ${prefix}_1.fq.gz"
+    def bgzip_command_2 = "echo | bgzip -c > ${prefix}_2.fq.gz"
+    def bgzip_command_other = "echo | bgzip -c > ${prefix}_other.fq.gz"
+    def bgzip_command_singleton = "echo | bgzip -c > ${prefix}_singleton.fq.gz"
+    def bgzip_command_interleaved = "echo | bgzip -c > ${prefix}_interleaved.fq.gz"
+
+    if (split) {
+        """
+        ${bgzip_command_1}
+        ${bgzip_command_2}
+        ${bgzip_command_other}
+        ${bgzip_command_singleton}
+        """
+    }
+    else {
+        """
+        ${bgzip_command_interleaved}
         """
     }
 }
diff --git a/modules/nf-core/samtools/bam2fq/meta.yml b/modules/nf-core/samtools/bam2fq/meta.yml
index b17ed608d0..1457ba3400 100644
--- a/modules/nf-core/samtools/bam2fq/meta.yml
+++ b/modules/nf-core/samtools/bam2fq/meta.yml
@@ -10,7 +10,8 @@ tools:
   - samtools:
       description: Tools for dealing with SAM, BAM and CRAM files
       documentation: http://www.htslib.org/doc/1.1/samtools.html
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:samtools
 input:
   - - meta:
@@ -22,17 +23,18 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
-  - - split:
-        type: boolean
-        description: |
-          TRUE/FALSE value to indicate if reads should be separated into
-          /1, /2 and if present other, or singleton.
-          Note: choosing TRUE will generate 4 different files.
-          Choosing FALSE will produce a single file, which will be interleaved in case
-          the input contains paired reads.
+        ontologies: []
+  - split:
+      type: boolean
+      description: |
+        TRUE/FALSE value to indicate if reads should be separated into
+        /1, /2 and if present other, or singleton.
+        Note: choosing TRUE will generate 4 different files.
+        Choosing FALSE will produce a single file, which will be interleaved in case
+        the input contains paired reads.
 output:
-  - reads:
-      - meta:
+  reads:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -43,12 +45,50 @@ output:
             FASTQ files, which will be either a group of 4 files (read_1, read_2, other and singleton)
             or a single interleaved .fq.gz file if the user chooses not to split the reads.
           pattern: "*.fq.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3989
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_bgzip:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bgzip:
+          type: string
+          description: The name of the tool
+      - bgzip --version | head -1 | sed "s/bgzip (htslib) //":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bgzip:
+          type: string
+          description: The name of the tool
+      - bgzip --version | head -1 | sed "s/bgzip (htslib) //":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@lescai"
 maintainers:
   - "@lescai"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/collatefastq/environment.yml b/modules/nf-core/samtools/collatefastq/environment.yml
index 62054fc97a..b08c7761d8 100644
--- a/modules/nf-core/samtools/collatefastq/environment.yml
+++ b/modules/nf-core/samtools/collatefastq/environment.yml
@@ -4,5 +4,8 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
+  - conda-forge::xz=5.8.3
diff --git a/modules/nf-core/samtools/collatefastq/main.nf b/modules/nf-core/samtools/collatefastq/main.nf
index 8b70ebd345..91b9020d7a 100644
--- a/modules/nf-core/samtools/collatefastq/main.nf
+++ b/modules/nf-core/samtools/collatefastq/main.nf
@@ -1,23 +1,23 @@
 process SAMTOOLS_COLLATEFASTQ {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/31/315d2445cd42b0f5512fa37965a9c59bc93ae8614b7d105150caece6c61e2e71/data'
+        : 'community.wave.seqera.io/library/htslib_samtools_xz:1595ae0727655963'}"
 
     input:
     tuple val(meta), path(input)
-    tuple val(meta2), path(fasta)
-    val(interleave)
+    tuple val(meta2), path(fasta), path(fai)
+    val interleave
 
     output:
-    tuple val(meta), path("*_{1,2}.fq.gz")     , optional:true, emit: fastq
-    tuple val(meta), path("*_interleaved.fq")  , optional:true, emit: fastq_interleaved
-    tuple val(meta), path("*_other.fq.gz")     , emit: fastq_other
-    tuple val(meta), path("*_singleton.fq.gz") , optional:true, emit: fastq_singleton
-    path "versions.yml"                        , emit: versions
+    tuple val(meta), path("*_{1,2}.fq.gz"), emit: fastq, optional: true
+    tuple val(meta), path("*_interleaved.fq"), emit: fastq_interleaved, optional: true
+    tuple val(meta), path("*_other.fq.gz"), emit: fastq_other
+    tuple val(meta), path("*_singleton.fq.gz"), emit: fastq_singleton, optional: true
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
@@ -27,50 +27,43 @@ process SAMTOOLS_COLLATEFASTQ {
     def args2 = task.ext.args2 ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
-    def output =    (interleave && ! meta.single_end) ? "> ${prefix}_interleaved.fq"                     :
-                    meta.single_end                   ? "-1 ${prefix}_1.fq.gz -s ${prefix}_singleton.fq.gz" :
-                    "-1 ${prefix}_1.fq.gz -2 ${prefix}_2.fq.gz -s ${prefix}_singleton.fq.gz"
+    def output = interleave && !meta.single_end
+        ? "> ${prefix}_interleaved.fq"
+        : meta.single_end
+            ? "-1 ${prefix}_1.fq.gz -s ${prefix}_singleton.fq.gz"
+            : "-1 ${prefix}_1.fq.gz -2 ${prefix}_2.fq.gz -s ${prefix}_singleton.fq.gz"
 
     """
     samtools collate \\
-        $args \\
-        --threads $task.cpus \\
+        ${args} \\
+        --threads ${task.cpus} \\
         ${reference} \\
         -O \\
-        $input \\
+        ${input} \\
         . |
 
     samtools fastq \\
-        $args2 \\
-        --threads $task.cpus \\
+        ${args2} \\
+        --threads ${task.cpus} \\
         ${reference} \\
         -0 ${prefix}_other.fq.gz \\
-        $output
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        ${output}
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def empty = "echo '' | gzip "
-    def singletoncommand = "${empty}> ${prefix}_singleton.fq.gz"
-    def interleavecommand = interleave && !meta.single_end ? "${empty}> ${prefix}_interleaved.fq.gz" : ""
-    def output1command = !interleave ? "${empty}> ${prefix}_1.fq.gz" : ""
-    def output2command = !interleave && !meta.single_end ? "${empty}> ${prefix}_2.fq.gz" : ""
+    def empty = "echo | bgzip -c "
+    def interleave_command = interleave && !meta.single_end ? "${empty}> ${prefix}_interleaved.fq.gz" : ""
+    def other_command = "${empty} > ${prefix}_other.fq.gz"
+    def output1_command = !interleave ? "${empty}> ${prefix}_1.fq.gz" : ""
+    def output2_command = !interleave && !meta.single_end ? "${empty}> ${prefix}_2.fq.gz" : ""
+    def singleton_command = "${empty}> ${prefix}_singleton.fq.gz"
 
     """
-    ${output1command}
-    ${output2command}
-    ${interleavecommand}
-    ${singletoncommand}
-    ${empty}> ${prefix}_other.fq.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+    ${interleave_command}
+    ${other_command}
+    ${output1_command}
+    ${output2_command}
+    ${singleton_command}
     """
 }
diff --git a/modules/nf-core/samtools/collatefastq/meta.yml b/modules/nf-core/samtools/collatefastq/meta.yml
index 5bc912496e..0086c9f94f 100644
--- a/modules/nf-core/samtools/collatefastq/meta.yml
+++ b/modules/nf-core/samtools/collatefastq/meta.yml
@@ -9,9 +9,13 @@ keywords:
 tools:
   - samtools:
       description: Tools for dealing with SAM, BAM and CRAM files
-      documentation: http://www.htslib.org/doc/1.1/samtools.html
+      homepage: "http://www.htslib.org"
+      documentation: "https://www.htslib.org/doc/samtools.html"
+      tool_dev_url: "https://github.com/samtools/samtools"
+      doi: "10.1093/bioinformatics/btp352"
       licence: ["MIT"]
       identifier: biotools:samtools
+
 input:
   - - meta:
         type: map
@@ -22,84 +26,102 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
           Groovy Map containing reference information
-          e.g. [ id:'test' ]
+          e.g. [ id:'genome' ]
     - fasta:
         type: file
-        description: Reference genome fasta file
-        pattern: "*.{fasta,fa}"
-  - - interleave:
-        type: boolean
-        description: |
-          If true, the output is a single interleaved paired-end FASTQ
-          If false, the output split paired-end FASTQ
-        default: false
+        description: Reference genome file
+        pattern: "*.{fa,fasta}"
+        ontologies: []
+    - fai:
+        type: file
+        description: Reference genome index file
+        pattern: "*.fai"
+        ontologies: []
+  - interleave:
+      type: boolean
+      description: |
+        If true, the output is a single interleaved paired-end FASTQ
+        If false, the output split paired-end FASTQ
+      default: false
 output:
-  - fastq:
-      - meta:
+  fastq:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*_{1,2}.fq.gz"
       - "*_{1,2}.fq.gz":
-          type: map
+          type: file
           description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+            R1 and R2 FASTQ files
           pattern: "*_{1,2}.fq.gz"
-  - fastq_interleaved:
-      - meta:
+          ontologies: []
+  fastq_interleaved:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*_interleaved.fq.gz"
       - "*_interleaved.fq":
-          type: map
+          type: file
           description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+            Interleaved paired end FASTQ files
           pattern: "*_interleaved.fq.gz"
-  - fastq_other:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  fastq_other:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*_other.fq.gz"
       - "*_other.fq.gz":
-          type: map
+          type: file
           description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+            FASTQ files with reads where the READ1 and READ2 FLAG bits set are either both set or both unset.
           pattern: "*_other.fq.gz"
-  - fastq_singleton:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  fastq_singleton:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-          pattern: "*_singleton.fq.gz"
       - "*_singleton.fq.gz":
-          type: map
+          type: file
           description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
+            FASTQ files with singleton reads.
           pattern: "*_singleton.fq.gz"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@lescai"
   - "@maxulysse"
   - "@matthdsm"
-maintainers:
-  - "@lescai"
-  - "@maxulysse"
-  - "@matthdsm"
diff --git a/modules/nf-core/samtools/convert/environment.yml b/modules/nf-core/samtools/convert/environment.yml
index 62054fc97a..6a19f168c2 100644
--- a/modules/nf-core/samtools/convert/environment.yml
+++ b/modules/nf-core/samtools/convert/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/convert/main.nf b/modules/nf-core/samtools/convert/main.nf
index 9667e72d84..61edbbf923 100644
--- a/modules/nf-core/samtools/convert/main.nf
+++ b/modules/nf-core/samtools/convert/main.nf
@@ -1,29 +1,28 @@
 process SAMTOOLS_CONVERT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
     tuple val(meta), path(input), path(index)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
+    tuple val(meta2), path(fasta), path(fai)
 
     output:
-    tuple val(meta), path("*.bam")  , emit: bam ,   optional: true
-    tuple val(meta), path("*.cram") , emit: cram,   optional: true
-    tuple val(meta), path("*.bai")  , emit: bai ,   optional: true
-    tuple val(meta), path("*.crai") , emit: crai,   optional: true
-    path  "versions.yml"            , emit: versions
+    tuple val(meta), path("*.bam"), emit: bam, optional: true
+    tuple val(meta), path("*.cram"), emit: cram, optional: true
+    tuple val(meta), path("*.bai"), emit: bai, optional: true
+    tuple val(meta), path("*.crai"), emit: crai, optional: true
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args  ?: ''
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def output_extension = input.getExtension() == "bam" ? "cram" : "bam"
 
@@ -31,16 +30,11 @@ process SAMTOOLS_CONVERT {
     samtools view \\
         --threads ${task.cpus} \\
         --reference ${fasta} \\
-        $args \\
-        $input \\
+        ${args} \\
+        ${input} \\
         -o ${prefix}.${output_extension}
 
     samtools index -@${task.cpus} ${prefix}.${output_extension}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -51,10 +45,5 @@ process SAMTOOLS_CONVERT {
     """
     touch ${prefix}.${output_extension}
     touch ${prefix}.${output_extension}.${index_extension}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/convert/meta.yml b/modules/nf-core/samtools/convert/meta.yml
index d5bfa161ba..286f812f1c 100644
--- a/modules/nf-core/samtools/convert/meta.yml
+++ b/modules/nf-core/samtools/convert/meta.yml
@@ -26,10 +26,12 @@ input:
         type: file
         description: BAM/CRAM file
         pattern: "*.{bam,cram}"
+        ontologies: []
     - index:
         type: file
         description: BAM/CRAM index file
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -39,18 +41,15 @@ input:
         type: file
         description: Reference file to create the CRAM file
         pattern: "*.{fasta,fa}"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
+        ontologies: []
     - fai:
         type: file
         description: Reference index file to create the CRAM file
         pattern: "*.{fai}"
+        ontologies: []
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -59,8 +58,9 @@ output:
           type: file
           description: filtered/converted BAM file
           pattern: "*{.bam}"
-  - cram:
-      - meta:
+          ontologies: []
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -69,8 +69,9 @@ output:
           type: file
           description: filtered/converted CRAM file
           pattern: "*{cram}"
-  - bai:
-      - meta:
+          ontologies: []
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -79,8 +80,9 @@ output:
           type: file
           description: filtered/converted BAM index
           pattern: "*{.bai}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -89,11 +91,28 @@ output:
           type: file
           description: filtered/converted CRAM index
           pattern: "*{.crai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
   - "@maxulysse"
diff --git a/modules/nf-core/samtools/faidx/environment.yml b/modules/nf-core/samtools/faidx/environment.yml
index 62054fc97a..6a19f168c2 100644
--- a/modules/nf-core/samtools/faidx/environment.yml
+++ b/modules/nf-core/samtools/faidx/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/faidx/main.nf b/modules/nf-core/samtools/faidx/main.nf
index 6de0095d86..3925ffafcb 100644
--- a/modules/nf-core/samtools/faidx/main.nf
+++ b/modules/nf-core/samtools/faidx/main.nf
@@ -1,23 +1,22 @@
 process SAMTOOLS_FAIDX {
-    tag "$fasta"
+    tag "${fasta}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
-    tuple val(meta), path(fasta)
-    tuple val(meta2), path(fai)
+    tuple val(meta), path(fasta), path(fai)
     val get_sizes
 
     output:
-    tuple val(meta), path ("*.{fa,fasta}") , emit: fa, optional: true
-    tuple val(meta), path ("*.sizes")      , emit: sizes, optional: true
-    tuple val(meta), path ("*.fai")        , emit: fai, optional: true
-    tuple val(meta), path ("*.gzi")        , emit: gzi, optional: true
-    path "versions.yml"                    , emit: versions
+    tuple val(meta), path("*.{fa,fasta}"), emit: fa, optional: true
+    tuple val(meta), path("*.sizes"), emit: sizes, optional: true
+    tuple val(meta), path("*.fai"), emit: fai, optional: true
+    tuple val(meta), path("*.gzi"), emit: gzi, optional: true
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
@@ -28,15 +27,10 @@ process SAMTOOLS_FAIDX {
     """
     samtools \\
         faidx \\
-        $fasta \\
-        $args
+        ${fasta} \\
+        ${args}
 
     ${get_sizes_command}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 
     stub:
@@ -51,11 +45,5 @@ process SAMTOOLS_FAIDX {
     fi
 
     ${get_sizes_command}
-
-    cat <<-END_VERSIONS > versions.yml
-
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/faidx/meta.yml b/modules/nf-core/samtools/faidx/meta.yml
index b7a2e0c1a3..529f7a2634 100644
--- a/modules/nf-core/samtools/faidx/meta.yml
+++ b/modules/nf-core/samtools/faidx/meta.yml
@@ -1,5 +1,6 @@
 name: samtools_faidx
-description: Index FASTA file, and optionally generate a file of chromosome sizes
+description: Index FASTA file, and optionally generate a file of chromosome
+  sizes
 keywords:
   - index
   - fasta
@@ -14,7 +15,8 @@ tools:
       homepage: http://www.htslib.org/
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:samtools
 input:
   - - meta:
@@ -27,11 +29,6 @@ input:
         description: FASTA file
         pattern: "*.{fa,fasta}"
         ontologies: []
-  - - meta2:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
     - fai:
         type: file
         description: FASTA index file
@@ -40,7 +37,6 @@ input:
   - get_sizes:
       type: boolean
       description: use cut to get the sizes of the index (true) or not (false)
-
 output:
   fa:
     - - meta:
@@ -86,13 +82,27 @@ output:
           description: Optional gzip index file for compressed inputs
           pattern: "*.gzi"
           ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: eval
+          description: The command used to generate the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: eval
+          description: The command used to generate the version of the tool
 authors:
   - "@drpatelh"
   - "@ewels"
@@ -100,3 +110,4 @@ authors:
 maintainers:
   - "@maxulysse"
   - "@phue"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml
index 62054fc97a..6a19f168c2 100644
--- a/modules/nf-core/samtools/index/environment.yml
+++ b/modules/nf-core/samtools/index/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf
index 311756102d..4dd9311a99 100644
--- a/modules/nf-core/samtools/index/main.nf
+++ b/modules/nf-core/samtools/index/main.nf
@@ -1,20 +1,18 @@
 process SAMTOOLS_INDEX {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
     tuple val(meta), path(input)
 
     output:
-    tuple val(meta), path("*.bai") , optional:true, emit: bai
-    tuple val(meta), path("*.csi") , optional:true, emit: csi
-    tuple val(meta), path("*.crai"), optional:true, emit: crai
-    path  "versions.yml"           , emit: versions
+    tuple val(meta), path("*.{bai,csi,crai}"), emit: index
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,26 +22,17 @@ process SAMTOOLS_INDEX {
     """
     samtools \\
         index \\
-        -@ ${task.cpus-1} \\
-        $args \\
-        $input
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        -@ ${task.cpus} \\
+        ${args} \\
+        ${input}
     """
 
     stub:
     def args = task.ext.args ?: ''
-    def extension = file(input).getExtension() == 'cram' ?
-                    "crai" : args.contains("-c") ?  "csi" : "bai"
+    def extension = file(input).getExtension() == 'cram'
+        ? "crai"
+        : args.contains("-c") ? "csi" : "bai"
     """
     touch ${input}.${extension}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml
index db8df0d505..d4938bcdfd 100644
--- a/modules/nf-core/samtools/index/meta.yml
+++ b/modules/nf-core/samtools/index/meta.yml
@@ -14,7 +14,8 @@ tools:
       homepage: http://www.htslib.org/
       documentation: http://www.htslib.org/doc/samtools.html
       doi: 10.1093/bioinformatics/btp352
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: biotools:samtools
 input:
   - - meta:
@@ -25,47 +26,45 @@ input:
     - input:
         type: file
         description: input file
+        ontologies: []
 output:
-  - bai:
-      - meta:
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.bai":
+      - "*.{bai,csi,crai}":
           type: file
           description: BAM/CRAM/SAM index file
-          pattern: "*.{bai,crai,sai}"
-  - csi:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: CSI index file
-          pattern: "*.{csi}"
-  - crai:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.crai":
-          type: file
-          description: BAM/CRAM/SAM index file
-          pattern: "*.{bai,crai,sai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          pattern: "*.{bai,csi,crai}"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@drpatelh"
   - "@ewels"
   - "@maxulysse"
 maintainers:
-  - "@drpatelh"
   - "@ewels"
   - "@maxulysse"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/merge/environment.yml b/modules/nf-core/samtools/merge/environment.yml
index 62054fc97a..6a19f168c2 100644
--- a/modules/nf-core/samtools/merge/environment.yml
+++ b/modules/nf-core/samtools/merge/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/merge/main.nf b/modules/nf-core/samtools/merge/main.nf
index 34da4c7c87..4d1fd305fc 100644
--- a/modules/nf-core/samtools/merge/main.nf
+++ b/modules/nf-core/samtools/merge/main.nf
@@ -1,61 +1,49 @@
 process SAMTOOLS_MERGE {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
-    tuple val(meta), path(input_files, stageAs: "?/*")
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fai)
+    tuple val(meta), path(input_files, stageAs: "?/*"), path(index_files, stageAs: "?/*")
+    tuple val(meta2), path(fasta), path(fai), path(gzi)
 
     output:
-    tuple val(meta), path("${prefix}.bam") , optional:true, emit: bam
-    tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram
-    tuple val(meta), path("*.csi")         , optional:true, emit: csi
-    tuple val(meta), path("*.crai")        , optional:true, emit: crai
-    path  "versions.yml"                                  , emit: versions
-
+    tuple val(meta), path("${prefix}.bam"), optional: true, emit: bam
+    tuple val(meta), path("${prefix}.cram"), optional: true, emit: cram
+    tuple val(meta), path("*.{bai,crai,csi}"), optional: true, emit: index
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), topic: versions, emit: versions_samtools
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args = task.ext.args   ?: ''
-    prefix   = task.ext.prefix ?: "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
     def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension()
     def reference = fasta ? "--reference ${fasta}" : ""
     """
+    # Note: --threads value represents *additional* CPUs to allocate (total CPUs = 1 + --threads).
     samtools \\
         merge \\
-        --threads ${task.cpus-1} \\
-        $args \\
+        --threads ${task.cpus - 1} \\
+        ${args} \\
         ${reference} \\
         ${prefix}.${file_type} \\
-        $input_files
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        ${input_files}
     """
 
     stub:
-    def args = task.ext.args   ?: ''
-    prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}"
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
     def file_type = input_files instanceof List ? input_files[0].getExtension() : input_files.getExtension()
     def index_type = file_type == "bam" ? "csi" : "crai"
     def index = args.contains("--write-index") ? "touch ${prefix}.${index_type}" : ""
     """
     touch ${prefix}.${file_type}
     ${index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/merge/meta.yml b/modules/nf-core/samtools/merge/meta.yml
index 235aa21945..66e582e870 100644
--- a/modules/nf-core/samtools/merge/meta.yml
+++ b/modules/nf-core/samtools/merge/meta.yml
@@ -26,6 +26,12 @@ input:
         type: file
         description: BAM/CRAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
+    - index_files:
+        type: file
+        description: BAI/CRAI/CSI index file
+        pattern: "*.{bai,crai,csi}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -35,18 +41,21 @@ input:
         type: file
         description: Reference file the CRAM was created with (optional)
         pattern: "*.{fasta,fa}"
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'genome' ]
+        ontologies: []
     - fai:
         type: file
         description: Index of the reference file the CRAM was created with (optional)
         pattern: "*.fai"
+        ontologies: []
+    - gzi:
+        type: file
+        description: Index of the compressed reference file the CRAM was created with
+          (optional)
+        pattern: "*.gzi"
+        ontologies: []
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -55,8 +64,9 @@ output:
           type: file
           description: BAM file
           pattern: "*.{bam}"
-  - cram:
-      - meta:
+          ontologies: []
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -65,40 +75,48 @@ output:
           type: file
           description: CRAM file
           pattern: "*.{cram}"
-  - csi:
-      - meta:
+          ontologies: []
+  index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
-      - "*.csi":
+      - "*.{bai,crai,csi}":
           type: file
           description: BAM index file (optional)
-          pattern: "*.csi"
-  - crai:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.crai":
-          type: file
-          description: CRAM index file (optional)
-          pattern: "*.crai"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          pattern: "*.{bai,crai,csi}"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: string
+          description: The command used to generate the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: string
+          description: The command used to generate the version of the tool
 authors:
-  - "@drpatelh"
-  - "@yuukiiwa "
+  - "@yuukiiwa"
   - "@maxulysse"
   - "@FriederikeHanssen"
   - "@ramprasadn"
 maintainers:
-  - "@drpatelh"
-  - "@yuukiiwa "
+  - "@yuukiiwa"
   - "@maxulysse"
   - "@FriederikeHanssen"
   - "@ramprasadn"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/mpileup/environment.yml b/modules/nf-core/samtools/mpileup/environment.yml
index 62054fc97a..b08c7761d8 100644
--- a/modules/nf-core/samtools/mpileup/environment.yml
+++ b/modules/nf-core/samtools/mpileup/environment.yml
@@ -4,5 +4,8 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
+  - conda-forge::xz=5.8.3
diff --git a/modules/nf-core/samtools/mpileup/main.nf b/modules/nf-core/samtools/mpileup/main.nf
index 8693aa0477..a6c5840628 100644
--- a/modules/nf-core/samtools/mpileup/main.nf
+++ b/modules/nf-core/samtools/mpileup/main.nf
@@ -1,51 +1,42 @@
 process SAMTOOLS_MPILEUP {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/31/315d2445cd42b0f5512fa37965a9c59bc93ae8614b7d105150caece6c61e2e71/data'
+        : 'community.wave.seqera.io/library/htslib_samtools_xz:1595ae0727655963'}"
 
     input:
-    tuple val(meta), path(input), path(intervals)
-    tuple val(meta2), path(fasta)
+    tuple val(meta), path(input), path(index), path(intervals)
+    tuple val(meta2), path(fasta), path(fai)
 
     output:
     tuple val(meta), path("*.mpileup.gz"), emit: mpileup
-    path  "versions.yml"                 , emit: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args          = task.ext.args   ?: ''
-    def prefix        = task.ext.prefix ?: "${meta.id}"
-    def fasta_cmd     = fasta           ? "--fasta-ref $fasta" : ""
-    def intervals_cmd = intervals       ? "-l ${intervals}" : ""
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def fasta_cmd = fasta ? "--fasta-ref ${fasta}" : ""
+    def intervals_cmd = intervals ? "-l ${intervals}" : ""
     """
     samtools mpileup \\
-        $fasta_cmd \\
+        ${fasta_cmd} \\
         --output ${prefix}.mpileup \\
-        $args \\
-        $intervals_cmd \\
-        $input
+        ${args} \\
+        ${intervals_cmd} \\
+        ${input}
     bgzip ${prefix}.mpileup
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
+    def bgzip_command = "echo | bgzip -c > ${prefix}.mpileup.gz"
     """
-    echo | gzip > ${prefix}.mpileup.gz
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+    ${bgzip_command}
     """
 }
diff --git a/modules/nf-core/samtools/mpileup/meta.yml b/modules/nf-core/samtools/mpileup/meta.yml
index 6195138ef0..a52a0ec09a 100644
--- a/modules/nf-core/samtools/mpileup/meta.yml
+++ b/modules/nf-core/samtools/mpileup/meta.yml
@@ -1,5 +1,6 @@
 name: samtools_mpileup
-description: BAM
+description: Generate text pileup output for one or multiple BAM files. Each
+  input file produces a separate group of pileup columns in the output.
 keywords:
   - mpileup
   - bam
@@ -26,22 +27,37 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
+    - index:
+        type: file
+        description: CSI/BAI/CRAI file. Optional. Only required when using the
+          '-r' parameter.
+        pattern: "*.{bai,crai,csi}"
+        ontologies: []
     - intervals:
         type: file
-        description: Interval FILE
+        description: Interval FILE. Optional.
         pattern: "*.bed"
+        ontologies: []
   - - meta2:
         type: map
         description: |
-          Groovy Map containing sample information
+          Groovy Map containing reference information
           e.g. [ id:'test' ]
     - fasta:
         type: file
-        description: FASTA reference file
-        pattern: "*.{fasta,fa}"
+        description: FASTA reference file. Optional.
+        pattern: "*.{fasta,fa,fna}"
+        ontologies: []
+    - fai:
+        type: file
+        description: FAI file. Optional. Only required (recommended) when using the
+          '-f' parameter.
+        pattern: "*.{fai}"
+        ontologies: []
 output:
-  - mpileup:
-      - meta:
+  mpileup:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -50,14 +66,32 @@ output:
           type: file
           description: mpileup file
           pattern: "*.{mpileup}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: string
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - samtools:
+          type: string
+          description: The tool name
+      - "samtools version | sed '1!d;s/.* //'":
+          type: string
+          description: The command used to generate the version of the tool
 authors:
   - "@drpatelh"
   - "@joseespinosa"
 maintainers:
-  - "@drpatelh"
   - "@joseespinosa"
+  - "@krannich479"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml
index 62054fc97a..6a19f168c2 100644
--- a/modules/nf-core/samtools/stats/environment.yml
+++ b/modules/nf-core/samtools/stats/environment.yml
@@ -4,5 +4,7 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  # renovate: datasource=conda depName=bioconda/htslib
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf
index 4443948b72..525ae00f96 100644
--- a/modules/nf-core/samtools/stats/main.nf
+++ b/modules/nf-core/samtools/stats/main.nf
@@ -1,48 +1,40 @@
 process SAMTOOLS_STATS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
     tuple val(meta), path(input), path(input_index)
-    tuple val(meta2), path(fasta)
+    tuple val(meta2), path(fasta), path(fai)
 
     output:
     tuple val(meta), path("*.stats"), emit: stats
-    path  "versions.yml"            , emit: versions
+    tuple val("${task.process}"), val('samtools'), eval('samtools version | sed "1!d;s/.* //"'), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
     """
     samtools \\
         stats \\
+        ${args} \\
         --threads ${task.cpus} \\
         ${reference} \\
         ${input} \\
         > ${prefix}.stats
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.stats
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml
index 77b020f76e..5fd7e76d99 100644
--- a/modules/nf-core/samtools/stats/meta.yml
+++ b/modules/nf-core/samtools/stats/meta.yml
@@ -27,10 +27,12 @@ input:
         type: file
         description: BAM/CRAM file from alignment
         pattern: "*.{bam,cram}"
+        ontologies: []
     - input_index:
         type: file
         description: BAI/CRAI file from alignment
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -39,10 +41,16 @@ input:
     - fasta:
         type: file
         description: Reference file the CRAM was created with (optional)
-        pattern: "*.{fasta,fa}"
+        pattern: "*.{fasta,fa,fna}"
+        ontologies: []
+    - fai:
+        type: file
+        description: FASTA ref index file
+        pattern: "*.{fasta,fa,fna}.fai"
+        ontologies: []
 output:
-  - stats:
-      - meta:
+  stats:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,11 +59,30 @@ output:
           type: file
           description: File containing samtools stats output
           pattern: "*.{stats}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: Name of the process
+      - samtools:
+          type: string
+          description: Name of the tool
+      - samtools version | sed "1!d;s/.* //":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: Name of the process
+      - samtools:
+          type: string
+          description: Name of the tool
+      - samtools version | sed "1!d;s/.* //":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@drpatelh"
   - "@FriederikeHanssen"
@@ -64,3 +91,4 @@ maintainers:
   - "@drpatelh"
   - "@FriederikeHanssen"
   - "@ramprasadn"
+  - "@matthdsm"
diff --git a/modules/nf-core/samtools/view/environment.yml b/modules/nf-core/samtools/view/environment.yml
index 8cae5712d5..6a19f168c2 100644
--- a/modules/nf-core/samtools/view/environment.yml
+++ b/modules/nf-core/samtools/view/environment.yml
@@ -5,5 +5,6 @@ channels:
   - bioconda
 dependencies:
   # renovate: datasource=conda depName=bioconda/htslib
-  - bioconda::htslib=1.21
-  - bioconda::samtools=1.21
+  - bioconda::htslib=1.24
+  # renovate: datasource=conda depName=bioconda/samtools
+  - bioconda::samtools=1.24
diff --git a/modules/nf-core/samtools/view/main.nf b/modules/nf-core/samtools/view/main.nf
index f43a4c6e72..459d4f24ac 100644
--- a/modules/nf-core/samtools/view/main.nf
+++ b/modules/nf-core/samtools/view/main.nf
@@ -1,28 +1,29 @@
 process SAMTOOLS_VIEW {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/samtools:1.21--h50ea8bc_0' :
-        'biocontainers/samtools:1.21--h50ea8bc_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/e9/e994bf4eb3731150511a14f5706b7bdfd64df1b6d40898fff334286c027e0859/data'
+        : 'community.wave.seqera.io/library/htslib_samtools:1.24--d697cfb9dce007cd'}"
 
     input:
     tuple val(meta), path(input), path(index)
-    tuple val(meta2), path(fasta)
-    path qname
+    tuple val(meta2), path(fasta), path(fai)
+    tuple val(meta3), path(qname)
+    tuple val(meta4), path(bed)
     val index_format
 
     output:
-    tuple val(meta), path("${prefix}.bam"),                                    emit: bam,              optional: true
-    tuple val(meta), path("${prefix}.cram"),                                   emit: cram,             optional: true
-    tuple val(meta), path("${prefix}.sam"),                                    emit: sam,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.bai"),                       emit: bai,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.csi"),                       emit: csi,              optional: true
-    tuple val(meta), path("${prefix}.${file_type}.crai"),                      emit: crai,             optional: true
-    tuple val(meta), path("${prefix}.unselected.${file_type}"),                emit: unselected,       optional: true
-    tuple val(meta), path("${prefix}.unselected.${file_type}.{csi,crai}"),     emit: unselected_index, optional: true
-    path  "versions.yml",                                                      emit: versions
+    tuple val(meta), path("${prefix}.bam"), emit: bam, optional: true
+    tuple val(meta), path("${prefix}.cram"), emit: cram, optional: true
+    tuple val(meta), path("${prefix}.sam"), emit: sam, optional: true
+    tuple val(meta), path("${prefix}.${file_type}.bai"), emit: bai, optional: true
+    tuple val(meta), path("${prefix}.${file_type}.csi"), emit: csi, optional: true
+    tuple val(meta), path("${prefix}.${file_type}.crai"), emit: crai, optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}"), emit: unselected, optional: true
+    tuple val(meta), path("${prefix}.unselected.${file_type}.{csi,crai}"), emit: unselected_index, optional: true
+    tuple val("${task.process}"), val('samtools'), eval('samtools version | sed "1!d;s/.* //"'), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -32,61 +33,71 @@ process SAMTOOLS_VIEW {
     def args2 = task.ext.args2 ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     def reference = fasta ? "--reference ${fasta}" : ""
-    file_type = args.contains("--output-fmt sam") ? "sam" :
-                args.contains("--output-fmt bam") ? "bam" :
-                args.contains("--output-fmt cram") ? "cram" :
-                input.getExtension()
+    file_type = args.contains("--output-fmt sam")
+        ? "sam"
+        : args.contains("--output-fmt bam")
+            ? "bam"
+            : args.contains("--output-fmt cram")
+                ? "cram"
+                : input.getExtension()
 
     output_file = index_format ? "${prefix}.${file_type}##idx##${prefix}.${file_type}.${index_format} --write-index" : "${prefix}.${file_type}"
     // Can't choose index type of unselected file
-    readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}": ""
+    readnames = qname ? "--qname-file ${qname} --output-unselected ${prefix}.unselected.${file_type}" : ""
+    def bedfile = bed ? "-L ${bed}" : ""
 
-    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${input}" == "${prefix}.${file_type}") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
     if (index_format) {
         if (!index_format.matches('bai|csi|crai')) {
-            error "Index format not one of bai, csi, crai."
-        } else if (file_type == "sam") {
-            error "Indexing not compatible with SAM output"
+            error("Index format not one of bai, csi, crai.")
+        }
+        else if (file_type == "sam") {
+            error("Indexing not compatible with SAM output")
         }
     }
     """
+    # Note: --threads value represents *additional* CPUs to allocate (total CPUs = 1 + --threads).
     samtools \\
         view \\
-        --threads ${task.cpus-1} \\
+        --threads ${task.cpus - 1} \\
         ${reference} \\
         ${readnames} \\
-        $args \\
+        ${bedfile} \\
+        ${args} \\
         -o ${output_file} \\
-        $input \\
-        $args2
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
+        ${input} \\
+        ${args2}
     """
 
     stub:
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
-    file_type = args.contains("--output-fmt sam") ? "sam" :
-                args.contains("--output-fmt bam") ? "bam" :
-                args.contains("--output-fmt cram") ? "cram" :
-                input.getExtension()
-    default_index_format =
-        file_type == "bam" ? "csi" :
-        file_type == "cram" ? "crai" : ""
-    index =  index_format ? "touch ${prefix}.${file_type}.${index_format}" : args.contains("--write-index") ? "touch ${prefix}.${file_type}.${default_index_format}" : ""
+    file_type = args.contains("--output-fmt sam")
+        ? "sam"
+        : args.contains("--output-fmt bam")
+            ? "bam"
+            : args.contains("--output-fmt cram")
+                ? "cram"
+                : input.getExtension()
+    default_index_format = file_type == "bam"
+        ? "csi"
+        : file_type == "cram" ? "crai" : ""
+    index = index_format ? "touch ${prefix}.${file_type}.${index_format}" : args.contains("--write-index") ? "touch ${prefix}.${file_type}.${default_index_format}" : ""
     unselected = qname ? "touch ${prefix}.unselected.${file_type}" : ""
     // Can't choose index type of unselected file
     unselected_index = qname && (args.contains("--write-index") || index_format) ? "touch ${prefix}.unselected.${file_type}.${default_index_format}" : ""
 
-    if ("$input" == "${prefix}.${file_type}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!"
+    if ("${input}" == "${prefix}.${file_type}") {
+        error("Input and output names are the same, use \"task.ext.prefix\" to disambiguate!")
+    }
     if (index_format) {
         if (!index_format.matches('bai|csi|crai')) {
-            error "Index format not one of bai, csi, crai."
-        } else if (file_type == "sam") {
-            error "Indexing not compatible with SAM output."
+            error("Index format not one of bai, csi, crai.")
+        }
+        else if (file_type == "sam") {
+            error("Indexing not compatible with SAM output.")
         }
     }
     """
@@ -94,10 +105,5 @@ process SAMTOOLS_VIEW {
     ${index}
     ${unselected}
     ${unselected_index}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/samtools/view/meta.yml b/modules/nf-core/samtools/view/meta.yml
index 28c268a657..51e3183436 100644
--- a/modules/nf-core/samtools/view/meta.yml
+++ b/modules/nf-core/samtools/view/meta.yml
@@ -26,30 +26,57 @@ input:
         type: file
         description: BAM/CRAM/SAM file
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
     - index:
         type: file
         description: BAM.BAI/BAM.CSI/CRAM.CRAI file (optional)
         pattern: "*.{.bai,.csi,.crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
           Groovy Map containing reference information
-          e.g. [ id:'test' ]
+          e.g. [ id:'genome' ]
     - fasta:
         type: file
-        description: Reference file the CRAM was created with (optional)
+        description: Fasta reference file
         pattern: "*.{fasta,fa}"
-  - - qname:
+        ontologies:
+          - edam: http://edamontology.org/format_1929 # FASTA
+    - fai:
+        type: file
+        description: Fasta reference file index
+        pattern: "*.{fai}"
+        ontologies:
+          - edam: http://edamontology.org/format_3326 # Index
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - qname:
         type: file
         description: Optional file with read names to output only select alignments
         pattern: "*.{txt,list}"
-  - - index_format:
-        type: string
-        description: Index format, used together with ext.args = '--write-index'
-        pattern: "bai|csi|crai"
+        ontologies: []
+  - - meta4:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'test', single_end:false ]
+    - bed:
+        type: file
+        description: Optional BED file for filtering alignments by genomic region (-L)
+        pattern: "*.{bed}"
+        ontologies:
+          - edam: http://edamontology.org/format_3003 # BED
+  - index_format:
+      type: string
+      description: Index format, used together with ext.args = '--write-index'
+      pattern: "bai|csi|crai"
 output:
-  - bam:
-      - meta:
+  bam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -58,8 +85,9 @@ output:
           type: file
           description: optional filtered/converted BAM file
           pattern: "*.{bam}"
-  - cram:
-      - meta:
+          ontologies: []
+  cram:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -68,8 +96,9 @@ output:
           type: file
           description: optional filtered/converted CRAM file
           pattern: "*.{cram}"
-  - sam:
-      - meta:
+          ontologies: []
+  sam:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -78,8 +107,9 @@ output:
           type: file
           description: optional filtered/converted SAM file
           pattern: "*.{sam}"
-  - bai:
-      - meta:
+          ontologies: []
+  bai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -88,8 +118,9 @@ output:
           type: file
           description: optional BAM file index
           pattern: "*.{bai}"
-  - csi:
-      - meta:
+          ontologies: []
+  csi:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -98,8 +129,9 @@ output:
           type: file
           description: optional tabix BAM file index
           pattern: "*.{csi}"
-  - crai:
-      - meta:
+          ontologies: []
+  crai:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -108,8 +140,9 @@ output:
           type: file
           description: optional CRAM file index
           pattern: "*.{crai}"
-  - unselected:
-      - meta:
+          ontologies: []
+  unselected:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -118,8 +151,9 @@ output:
           type: file
           description: optional file with unselected alignments
           pattern: "*.unselected.{bam,cram,sam}"
-  - unselected_index:
-      - meta:
+          ontologies: []
+  unselected_index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -128,11 +162,30 @@ output:
           type: file
           description: index for the "unselected" file
           pattern: "*.unselected.{csi,crai}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: Name of the process
+      - samtools:
+          type: string
+          description: Name of the tool
+      - samtools version | sed "1!d;s/.* //":
+          type: eval
+          description: The expression to obtain the version of the tool
+
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: Name of the process
+      - samtools:
+          type: string
+          description: Name of the tool
+      - samtools version | sed "1!d;s/.* //":
+          type: eval
+          description: The expression to obtain the version of the tool
+
 authors:
   - "@drpatelh"
   - "@joseespinosa"
@@ -143,3 +196,4 @@ maintainers:
   - "@joseespinosa"
   - "@FriederikeHanssen"
   - "@priyanka-surana"
+  - "@matthdsm"
diff --git a/subworkflows/local/bam_applybqsr/main.nf b/subworkflows/local/bam_applybqsr/main.nf
index 0814ed4e2c..a573c0393d 100644
--- a/subworkflows/local/bam_applybqsr/main.nf
+++ b/subworkflows/local/bam_applybqsr/main.nf
@@ -17,7 +17,6 @@ workflow BAM_APPLYBQSR {
     intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     // Move num_intervals to meta map
@@ -54,11 +53,6 @@ workflow BAM_APPLYBQSR {
         .mix(CRAM_MERGE_INDEX_SAMTOOLS.out.cram_crai)
         .map { meta, file_, index -> [meta - meta.subMap('num_intervals'), file_, index] }
 
-    // Gather versions of all tools used
-    versions = versions.mix(BAM_MERGE_INDEX_SAMTOOLS.out.versions)
-    versions = versions.mix(CRAM_MERGE_INDEX_SAMTOOLS.out.versions)
-
     emit:
     alignment = recal_out // channel: [ meta, file, index ] — BAM or CRAM
-    versions              // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_applybqsr_spark/main.nf b/subworkflows/local/bam_applybqsr_spark/main.nf
index 2948e97b04..52eb0177d9 100644
--- a/subworkflows/local/bam_applybqsr_spark/main.nf
+++ b/subworkflows/local/bam_applybqsr_spark/main.nf
@@ -17,7 +17,6 @@ workflow BAM_APPLYBQSR_SPARK {
     intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     // Move num_intervals to meta map
@@ -54,11 +53,6 @@ workflow BAM_APPLYBQSR_SPARK {
         .mix(CRAM_MERGE_INDEX_SAMTOOLS.out.cram_crai)
         .map { meta, file_, index -> [meta - meta.subMap('num_intervals'), file_, index] }
 
-    // Gather versions of all tools used
-    versions = versions.mix(BAM_MERGE_INDEX_SAMTOOLS.out.versions)
-    versions = versions.mix(CRAM_MERGE_INDEX_SAMTOOLS.out.versions)
-
     emit:
     alignment = recal_out // channel: [ meta, file, index ] — BAM or CRAM
-    versions              // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_convert_samtools/main.nf b/subworkflows/local/bam_convert_samtools/main.nf
index 56ee28f72b..8dc9746630 100644
--- a/subworkflows/local/bam_convert_samtools/main.nf
+++ b/subworkflows/local/bam_convert_samtools/main.nf
@@ -14,26 +14,26 @@ include { CAT_FASTQ                                          } from '../../../mo
 workflow BAM_CONVERT_SAMTOOLS {
     take:
     input       // channel: [meta, alignment (BAM or CRAM), index (optional)]
-    fasta       // optional: reference file if CRAM format and reference not in header
-    fasta_fai
+    fasta       // channel: [meta, fasta] — reference, staged in to decode CRAM input
+    fasta_fai   // channel: [meta, fai]
     interleaved // value: true/false
 
     main:
-    versions = channel.empty()
 
-    // Index File if not PROVIDED -> this also requires updates to samtools view possibly URGH
+    // Combined [ meta, fasta, fai ] reference tuple for the updated samtools modules
+    fasta_and_fai = fasta.combine(fasta_fai).map { meta, fasta_, _fai_meta, fai -> [ meta, fasta_, fai ] }.collect()
 
     // MAP - MAP
-    SAMTOOLS_VIEW_MAP_MAP(input, fasta, [], [])
+    SAMTOOLS_VIEW_MAP_MAP(input, fasta_and_fai, [[], []], [[], []], [])
 
     // UNMAP - UNMAP
-    SAMTOOLS_VIEW_UNMAP_UNMAP(input, fasta, [], [])
+    SAMTOOLS_VIEW_UNMAP_UNMAP(input, fasta_and_fai, [[], []], [[], []], [])
 
     // UNMAP - MAP
-    SAMTOOLS_VIEW_UNMAP_MAP(input, fasta, [], [])
+    SAMTOOLS_VIEW_UNMAP_MAP(input, fasta_and_fai, [[], []], [[], []], [])
 
     // MAP - UNMAP
-    SAMTOOLS_VIEW_MAP_UNMAP(input, fasta, [], [])
+    SAMTOOLS_VIEW_MAP_UNMAP(input, fasta_and_fai, [[], []], [[], []], [])
 
     // Merge UNMAP
     all_unmapped_bam = SAMTOOLS_VIEW_UNMAP_UNMAP.out.bam
@@ -41,13 +41,13 @@ workflow BAM_CONVERT_SAMTOOLS {
         .join(SAMTOOLS_VIEW_MAP_UNMAP.out.bam, failOnDuplicate: true, remainder: true)
         .map{ meta, unmap_unmap, unmap_map, map_unmap -> [ meta, [ unmap_unmap, unmap_map, map_unmap ] ] }
 
-    SAMTOOLS_MERGE_UNMAP(all_unmapped_bam, fasta, fasta_fai)
+    SAMTOOLS_MERGE_UNMAP(all_unmapped_bam.map { meta, bams -> [ meta, bams, [] ] }, fasta_and_fai.map { meta, fasta_, fai -> [ meta, fasta_, fai, [] ] })
 
     // Collate & convert unmapped
-    COLLATE_FASTQ_UNMAP(SAMTOOLS_MERGE_UNMAP.out.bam, fasta, interleaved)
+    COLLATE_FASTQ_UNMAP(SAMTOOLS_MERGE_UNMAP.out.bam, fasta_and_fai, interleaved)
 
     // Collate & convert mapped
-    COLLATE_FASTQ_MAP(SAMTOOLS_VIEW_MAP_MAP.out.bam, fasta, interleaved)
+    COLLATE_FASTQ_MAP(SAMTOOLS_VIEW_MAP_MAP.out.bam, fasta_and_fai, interleaved)
 
     // join Mapped & unmapped fastq
 
@@ -59,17 +59,7 @@ workflow BAM_CONVERT_SAMTOOLS {
     CAT_FASTQ(reads_to_concat)
     reads = CAT_FASTQ.out.reads
 
-    // Gather versions of all tools used
-    versions = versions.mix(COLLATE_FASTQ_MAP.out.versions)
-    versions = versions.mix(COLLATE_FASTQ_UNMAP.out.versions)
-    versions = versions.mix(SAMTOOLS_MERGE_UNMAP.out.versions)
-    versions = versions.mix(SAMTOOLS_VIEW_MAP_MAP.out.versions)
-    versions = versions.mix(SAMTOOLS_VIEW_MAP_UNMAP.out.versions)
-    versions = versions.mix(SAMTOOLS_VIEW_UNMAP_MAP.out.versions)
-    versions = versions.mix(SAMTOOLS_VIEW_UNMAP_UNMAP.out.versions)
-
     emit:
     reads
 
-    versions
 }
diff --git a/subworkflows/local/bam_markduplicates/main.nf b/subworkflows/local/bam_markduplicates/main.nf
index b1501ee40c..2e770e732c 100644
--- a/subworkflows/local/bam_markduplicates/main.nf
+++ b/subworkflows/local/bam_markduplicates/main.nf
@@ -30,7 +30,7 @@ workflow BAM_MARKDUPLICATES {
             .join(GATK4_MARKDUPLICATES.out.crai, failOnDuplicate: true, failOnMismatch: true))
 
     // QC on alignment
-    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, intervals_bed_combined)
+    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, fasta_fai, intervals_bed_combined)
 
     // Gather all reports generated
     reports = reports.mix(GATK4_MARKDUPLICATES.out.metrics)
diff --git a/subworkflows/local/bam_markduplicates_spark/main.nf b/subworkflows/local/bam_markduplicates_spark/main.nf
index 86a3eaadb8..0c9172b706 100644
--- a/subworkflows/local/bam_markduplicates_spark/main.nf
+++ b/subworkflows/local/bam_markduplicates_spark/main.nf
@@ -29,10 +29,10 @@ workflow BAM_MARKDUPLICATES_SPARK {
 
     // Unified alignment output — join with the appropriate index
     alignment = GATK4SPARK_MARKDUPLICATES.out.output
-        .join(INDEX_MARKDUPLICATES.out.bai.mix(INDEX_MARKDUPLICATES.out.crai), failOnDuplicate: true, failOnMismatch: true)
+        .join(INDEX_MARKDUPLICATES.out.index, failOnDuplicate: true, failOnMismatch: true)
 
     // QC on alignment
-    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, intervals_bed_combined)
+    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, fasta_fai, intervals_bed_combined)
 
     // When running Marduplicates spark, and saving reports
     GATK4_ESTIMATELIBRARYCOMPLEXITY(bam, fasta.map{ _meta, fasta_ -> [ fasta_ ] }, fasta_fai.map{ _meta, fasta_fai_ -> [ fasta_fai_ ] }, dict.map{ _meta, dict_ -> [ dict_ ] })
@@ -42,7 +42,6 @@ workflow BAM_MARKDUPLICATES_SPARK {
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
     // Gather versions of all tools used
-    versions = versions.mix(INDEX_MARKDUPLICATES.out.versions)
     versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
 
     emit:
diff --git a/subworkflows/local/bam_merge_index_samtools/main.nf b/subworkflows/local/bam_merge_index_samtools/main.nf
index 61d252d226..41c7797211 100644
--- a/subworkflows/local/bam_merge_index_samtools/main.nf
+++ b/subworkflows/local/bam_merge_index_samtools/main.nf
@@ -12,7 +12,6 @@ workflow BAM_MERGE_INDEX_SAMTOOLS {
     bam // channel: [mandatory] meta, bam
 
     main:
-    versions = channel.empty()
 
     // Figuring out if there is one or more bam(s) from the same sample
     bam_to_merge = bam.branch{ meta, bam_ ->
@@ -23,7 +22,7 @@ workflow BAM_MERGE_INDEX_SAMTOOLS {
     }
 
     // Only when using intervals
-    MERGE_BAM(bam_to_merge.multiple, [ [ id:'null' ], []], [ [ id:'null' ], []])
+    MERGE_BAM(bam_to_merge.multiple.map { meta, bams -> [ meta, bams, [] ] }, [ [ id:'null' ], [], [], [] ])
 
     // Mix intervals and no_intervals channels together
     bam_all = MERGE_BAM.out.bam.mix(bam_to_merge.single)
@@ -32,14 +31,9 @@ workflow BAM_MERGE_INDEX_SAMTOOLS {
     INDEX_MERGE_BAM(bam_all)
 
     // Join with the bai file
-    bam_bai = bam_all.join(INDEX_MERGE_BAM.out.bai, failOnDuplicate: true, failOnMismatch: true)
-
-    // Gather versions of all tools used
-    versions = versions.mix(INDEX_MERGE_BAM.out.versions)
-    versions = versions.mix(MERGE_BAM.out.versions)
+    bam_bai = bam_all.join(INDEX_MERGE_BAM.out.index, failOnDuplicate: true, failOnMismatch: true)
 
     emit:
     bam_bai
 
-    versions
 }
diff --git a/subworkflows/local/bam_sentieon_dedup/main.nf b/subworkflows/local/bam_sentieon_dedup/main.nf
index 33ffffd79d..151febcc39 100644
--- a/subworkflows/local/bam_sentieon_dedup/main.nf
+++ b/subworkflows/local/bam_sentieon_dedup/main.nf
@@ -30,7 +30,7 @@ workflow BAM_SENTIEON_DEDUP {
         : SENTIEON_DEDUP.out.cram.join(SENTIEON_DEDUP.out.crai, failOnDuplicate: true, failOnMismatch: true)
 
     // QC on alignment
-    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, intervals_bed_combined)
+    CRAM_QC_MOSDEPTH_SAMTOOLS(alignment, fasta, fasta_fai, intervals_bed_combined)
 
     // Gather all reports generated
     reports = reports.mix(SENTIEON_DEDUP.out.metrics)
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index 2fe3ed51c6..1a1c7af1fc 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -91,7 +91,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         )
         vcf_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.vcf
         tbi_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_MPILEUP.out.versions)
     }
 
     // CNVKIT
@@ -153,7 +152,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         vcf_haplotypecaller = BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.vcf
         tbi_haplotypecaller = BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.tbi
 
-
         if (joint_germline) {
             BAM_JOINT_CALLING_GERMLINE_GATK(
                 BAM_VARIANT_CALLING_HAPLOTYPECALLER.out.gvcf_tbi_intervals,
diff --git a/subworkflows/local/bam_variant_calling_mpileup/main.nf b/subworkflows/local/bam_variant_calling_mpileup/main.nf
index 125a4a4684..2f94463936 100644
--- a/subworkflows/local/bam_variant_calling_mpileup/main.nf
+++ b/subworkflows/local/bam_variant_calling_mpileup/main.nf
@@ -18,7 +18,6 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
 
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = cram
@@ -28,6 +27,9 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     // per-chunk bed restricts mpileup only; intervals_call left empty
     cram_intervals_bcftools = cram_intervals.map { meta, cram_, intervals_ -> [meta, cram_, intervals_, []] }
 
+    // samtools/mpileup now expects [ meta, input, index, intervals ]; keep the crai
+    cram_crai_intervals = cram.combine(intervals).map { meta, cram_, crai, intervals_, num_intervals -> [meta + [num_intervals: num_intervals], cram_, crai, intervals_] }
+
     fasta_fai = fasta.combine(fai).map { meta, fasta_, _meta2, fai_ -> [meta, fasta_, fai_] }.collect()
 
     // Run, if --tools mpileup
@@ -35,7 +37,7 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
     BCFTOOLS_MPILEUP(cram_intervals_bcftools, fasta_fai, keep_bcftools_mpileup)
 
     //Only run, if --tools ControlFreec
-    SAMTOOLS_MPILEUP(cram_intervals, fasta)
+    SAMTOOLS_MPILEUP(cram_crai_intervals, fasta_fai)
 
     // Figuring out if there is one or more vcf(s) from the same sample
     vcf_mpileup = BCFTOOLS_MPILEUP.out.vcf.branch { meta, _vcf ->
@@ -74,11 +76,8 @@ workflow BAM_VARIANT_CALLING_MPILEUP {
         .mix(tbi_mpileup.no_intervals)
         .map { meta, tbi -> [meta - meta.subMap('num_intervals') + [variantcaller: 'bcftools'], tbi] }
 
-    versions = versions.mix(SAMTOOLS_MPILEUP.out.versions)
-
     emit:
     mpileup
     vcf
     tbi
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index 10a7b40b8f..4ca65663ad 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -122,8 +122,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             wes ? intervals_bed_combined : [],
         )
 
-        versions = versions.mix(MPILEUP_NORMAL.out.versions)
-        versions = versions.mix(MPILEUP_TUMOR.out.versions)
     }
 
     // CNVKIT
@@ -167,7 +165,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
         tbi_manta = BAM_VARIANT_CALLING_SOMATIC_MANTA.out.diploid_sv_vcf_tbi.mix(BAM_VARIANT_CALLING_SOMATIC_MANTA.out.somatic_sv_vcf_tbi)
     }
 
-
     // INDEXCOV
     //   WGS only
     if (params.wes == false && tools.split(',').contains('indexcov')) {
@@ -181,7 +178,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
         versions = versions.mix(BAM_VARIANT_CALLING_INDEXCOV.out.versions)
     }
 
-
     // STRELKA
     if (tools && tools.split(',').contains('strelka')) {
         cram_strelka = tools.split(',').contains('manta')
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 12e2e570e5..9b83123f0f 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -76,7 +76,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
         )
         vcf_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.vcf
         tbi_mpileup = BAM_VARIANT_CALLING_MPILEUP.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_MPILEUP.out.versions)
     }
 
     // CONTROLFREEC (depends on MPILEUP)
diff --git a/subworkflows/local/cram_merge_index_samtools/main.nf b/subworkflows/local/cram_merge_index_samtools/main.nf
index def52fc2c5..93972d6b15 100644
--- a/subworkflows/local/cram_merge_index_samtools/main.nf
+++ b/subworkflows/local/cram_merge_index_samtools/main.nf
@@ -14,7 +14,6 @@ workflow CRAM_MERGE_INDEX_SAMTOOLS {
     fasta_fai // channel: [mandatory] meta, fai
 
     main:
-    versions = channel.empty()
 
     // Figuring out if there is one or more cram(s) from the same sample
     cram_to_merge = cram.branch { meta, cram_files ->
@@ -24,7 +23,7 @@ workflow CRAM_MERGE_INDEX_SAMTOOLS {
     }
 
     // Only when using intervals
-    MERGE_CRAM(cram_to_merge.multiple, fasta, fasta_fai)
+    MERGE_CRAM(cram_to_merge.multiple.map { meta, crams -> [ meta, crams, [] ] }, fasta.combine(fasta_fai).map { meta, fasta_, _meta_fai, fai -> [ meta, fasta_, fai, [] ] }.collect())
 
     // Mix intervals and no_intervals channels together
     cram_all = MERGE_CRAM.out.cram.mix(cram_to_merge.single)
@@ -33,13 +32,8 @@ workflow CRAM_MERGE_INDEX_SAMTOOLS {
     INDEX_CRAM(cram_all)
 
     // Join with the crai file
-    cram_crai = cram_all.join(INDEX_CRAM.out.crai, failOnDuplicate: true, failOnMismatch: true)
-
-    // Gather versions of all tools used
-    versions = versions.mix(INDEX_CRAM.out.versions)
-    versions = versions.mix(MERGE_CRAM.out.versions)
+    cram_crai = cram_all.join(INDEX_CRAM.out.index, failOnDuplicate: true, failOnMismatch: true)
 
     emit:
     cram_crai
-    versions
 }
diff --git a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
index 15b1e97c4d..d5a1371cb3 100644
--- a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
+++ b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
@@ -10,7 +10,8 @@ include { MOSDEPTH       } from '../../../modules/nf-core/mosdepth/main'
 workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
     take:
     cram      // channel: [mandatory] [ meta, cram, crai ]
-    fasta     // channel: [mandatory] [ fasta ]
+    fasta     // channel: [mandatory] [ meta, fasta ]
+    fasta_fai // channel: [mandatory] [ meta, fai ]
     intervals
 
     main:
@@ -18,19 +19,15 @@ workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
     reports = channel.empty()
 
     // Reports run on cram
-    SAMTOOLS_STATS(cram, fasta)
+    SAMTOOLS_STATS(cram, fasta.combine(fasta_fai).map { meta, fasta_, _fai_meta, fai -> [ meta, fasta_, fai ] }.collect())
 
-    MOSDEPTH(cram.combine(intervals.map { _meta, bed -> [bed ?: []] }), fasta)
+    MOSDEPTH(cram.combine(intervals.map { _meta, bed -> [bed ?: []] }), fasta, [])
 
     // Gather all reports generated
     reports = reports.mix(SAMTOOLS_STATS.out.stats)
     reports = reports.mix(MOSDEPTH.out.global_txt)
     reports = reports.mix(MOSDEPTH.out.regions_txt)
 
-    // Gather versions of all tools used
-    versions = versions.mix(MOSDEPTH.out.versions)
-    versions = versions.mix(SAMTOOLS_STATS.out.versions)
-
     emit:
     reports
     versions // channel: [ versions.yml ]
diff --git a/subworkflows/local/cram_sampleqc/main.nf b/subworkflows/local/cram_sampleqc/main.nf
index c3be937964..4ac78c2af8 100644
--- a/subworkflows/local/cram_sampleqc/main.nf
+++ b/subworkflows/local/cram_sampleqc/main.nf
@@ -18,6 +18,7 @@ workflow CRAM_SAMPLEQC {
         CRAM_QC_RECAL(
             cram,
             fasta_fai.map{meta, fasta, _fai -> [meta, fasta]},
+            fasta_fai.map{meta, _fasta, fai -> [meta, fai]},
             intervals_for_preprocessing,
         )
 
diff --git a/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf b/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
index f173a52108..11a5b13d72 100644
--- a/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
+++ b/subworkflows/local/fastq_create_umi_consensus_fgbio/main.nf
@@ -22,7 +22,6 @@ workflow FASTQ_CREATE_UMI_CONSENSUS_FGBIO {
     groupreadsbyumi_strategy  // string:  [mandatory] grouping strategy - default: "Adjacency"
 
     main:
-    ch_versions = channel.empty()
 
     // params.umi_read_structure is passed out as ext.args
     // FASTQ reads are converted into a tagged unmapped BAM file (uBAM)
@@ -57,7 +56,7 @@ workflow FASTQ_CREATE_UMI_CONSENSUS_FGBIO {
         }
 
     // Merge across runs/lanes for the same sample
-    MERGE_CONSENSUS(bams_to_merge.multiple, [[], []], [[], []])
+    MERGE_CONSENSUS(bams_to_merge.multiple.map { meta, bams -> [ meta, bams, [] ] }, [[], [], [], []])
 
     bams_all = MERGE_CONSENSUS.out.bam.mix(bams_to_merge.single)
 
@@ -72,12 +71,8 @@ workflow FASTQ_CREATE_UMI_CONSENSUS_FGBIO {
     call_min_baseq = 10
     CALLUMICONSENSUS(GROUPREADSBYUMI.out.bam, call_min_reads, call_min_baseq)
 
-    ch_versions = ch_versions.mix(BAM2FASTQ.out.versions)
-    ch_versions = ch_versions.mix(MERGE_CONSENSUS.out.versions)
-
     emit:
     umibam         = FASTQTOBAM.out.bam             // channel: [ val(meta), [ bam ] ]
     groupbam       = GROUPREADSBYUMI.out.bam        // channel: [ val(meta), [ bam ] ]
     consensusbam   = CALLUMICONSENSUS.out.bam       // channel: [ val(meta), [ bam ] ]
-    versions       = ch_versions                    // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index df793c1de8..6fdb2613ee 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -90,15 +90,13 @@ workflow FASTQ_PREPROCESS_GATK {
             interleave_input = false // Currently don't allow interleaved input
             CONVERT_FASTQ_UMI(
                 bam_converted_from_fastq,
-                [ [ id:"fasta" ], [] ], // fasta
-                [ [ id:'null' ], [] ],  // fasta_fai
+                fasta,
+                fasta_fai,
                 interleave_input)
 
             reads_for_fastp = CONVERT_FASTQ_UMI.out.reads
 
             // Gather used softwares versions
-            versions = versions.mix(CONVERT_FASTQ_UMI.out.versions)
-            versions = versions.mix(FASTQ_CREATE_UMI_CONSENSUS_FGBIO.out.versions)
         } else {
             reads_for_fastp = input_fastq
         }
@@ -125,7 +123,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 }.transpose()
             } else reads_for_bbsplit = FASTP.out.reads
 
-
         } else {
             reads_for_bbsplit = reads_for_fastp
         }
@@ -150,7 +147,6 @@ workflow FASTQ_PREPROCESS_GATK {
             reads_for_alignment = reads_for_bbsplit
         }
 
-
         // STEP 1: MAPPING READS TO REFERENCE GENOME
         // First, we must calculate number of lanes for each sample (meta.n_fastq)
         // This is needed to group reads from the same sample together using groupKey to avoid stalling the workflow
@@ -224,7 +220,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Group
             .groupTuple()
 
-
         // gatk4 markduplicates can handle multiple bams as input, so no need to merge/index here
         // Except if and only if save_mapped or (skipping markduplicates and sentieon-dedup)
         if (
@@ -237,14 +232,12 @@ workflow FASTQ_PREPROCESS_GATK {
             // bams are merged (when multiple lanes from the same sample), indexed and then converted to cram
             BAM_MERGE_INDEX_SAMTOOLS(bam_mapped)
 
-            BAM_TO_CRAM_MAPPING(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, fasta, fasta_fai)
+            BAM_TO_CRAM_MAPPING(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, fasta.combine(fasta_fai).map { meta, fasta_, _meta_fai, fai -> [ meta, fasta_, fai ] }.collect())
             // Create CSV to restart from this step
             if (params.save_output_as_bam) CHANNEL_ALIGN_CREATE_CSV(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, params.outdir, params.save_output_as_bam)
             else CHANNEL_ALIGN_CREATE_CSV(BAM_TO_CRAM_MAPPING.out.cram.join(BAM_TO_CRAM_MAPPING.out.crai, failOnDuplicate: true, failOnMismatch: true), params.outdir, params.save_output_as_bam)
 
             // Gather used softwares versions
-            versions = versions.mix(BAM_MERGE_INDEX_SAMTOOLS.out.versions)
-            versions = versions.mix(BAM_TO_CRAM_MAPPING.out.versions)
         }
 
     }
@@ -285,7 +278,7 @@ workflow FASTQ_PREPROCESS_GATK {
                 cram_skip_markduplicates = channel.empty().mix(input_sample)
             }
 
-            CRAM_QC_NO_MD(cram_skip_markduplicates, fasta, intervals_for_preprocessing)
+            CRAM_QC_NO_MD(cram_skip_markduplicates, fasta, fasta_fai, intervals_for_preprocessing)
 
             // Gather QC reports
             reports = reports.mix(CRAM_QC_NO_MD.out.reports.collect{ _meta, report -> [ report ] })
@@ -456,7 +449,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 cram_variant_calling_spark = BAM_APPLYBQSR_SPARK.out.alignment
 
                 // Gather used softwares versions
-                versions = versions.mix(BAM_APPLYBQSR_SPARK.out.versions)
 
             } else {
 
@@ -470,7 +462,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 cram_variant_calling_no_spark = BAM_APPLYBQSR.out.alignment
 
                 // Gather used softwares versions
-                versions = versions.mix(BAM_APPLYBQSR.out.versions)
             }
 
             cram_variant_calling = channel.empty().mix(
diff --git a/subworkflows/local/fastq_preprocess_parabricks/main.nf b/subworkflows/local/fastq_preprocess_parabricks/main.nf
index 8886d0611f..d4a15fb271 100644
--- a/subworkflows/local/fastq_preprocess_parabricks/main.nf
+++ b/subworkflows/local/fastq_preprocess_parabricks/main.nf
@@ -18,7 +18,6 @@ workflow FASTQ_PREPROCESS_PARABRICKS {
     val_outdir                      // output directory for saving mapped files
 
     main:
-    ch_versions = channel.empty()
     ch_reports  = channel.empty()
 
     reads_grouping_key = ch_reads.map { meta, reads ->
@@ -78,8 +77,6 @@ workflow FASTQ_PREPROCESS_PARABRICKS {
     // crams are merged (when multiple lanes from the same sample) and indexed
     CRAM_MERGE_INDEX_SAMTOOLS(cram_mapped, ch_fasta, ch_fasta_fai)
 
-    ch_versions = ch_versions.mix(CRAM_MERGE_INDEX_SAMTOOLS.out.versions)
-
     cram_variant_calling = CRAM_MERGE_INDEX_SAMTOOLS.out.cram_crai
         .map { meta, cram, crai ->
                 [ meta - meta.subMap('id', 'read_group', 'data_type', 'size', 'sample_lane_id', 'lane') + [ data_type: 'cram', id: meta.sample ], cram, crai ]
@@ -87,8 +84,7 @@ workflow FASTQ_PREPROCESS_PARABRICKS {
 
     if (val_save_output_as_bam) {
         // Convert CRAM files to BAM
-        CRAM_TO_BAM(cram_variant_calling, ch_fasta, ch_fasta_fai)
-        ch_versions = ch_versions.mix(CRAM_TO_BAM.out.versions)
+        CRAM_TO_BAM(cram_variant_calling, ch_fasta.combine(ch_fasta_fai).map { meta, fasta_, _meta_fai, fai -> [ meta, fasta_, fai ] }.collect())
         CHANNEL_ALIGN_CREATE_CSV(CRAM_TO_BAM.out.bam.join(CRAM_TO_BAM.out.bai, failOnDuplicate: true, failOnMismatch: true), val_outdir, val_save_output_as_bam)
     } else if (val_save_mapped) {
         CHANNEL_ALIGN_CREATE_CSV(cram_variant_calling, val_outdir, val_save_output_as_bam)
@@ -96,6 +92,5 @@ workflow FASTQ_PREPROCESS_PARABRICKS {
 
     emit:
     cram      = cram_variant_calling     // channel: [ val(meta), cram, crai ]
-    versions  = ch_versions              // channel: [ versions.yml ]
     reports   = ch_reports
 }
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 5193807d1d..c52b73d911 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -99,9 +99,8 @@ workflow PREPARE_GENOME {
     }
 
     if (!fasta_fai_in && step != "annotate") {
-        SAMTOOLS_FAIDX(fasta, [[id: 'no_fai'], []], false)
+        SAMTOOLS_FAIDX(fasta.map { meta, fasta_ -> [ meta, fasta_, [] ] }, false)
         fasta_fai = SAMTOOLS_FAIDX.out.fai.collect()
-        versions = versions.mix(SAMTOOLS_FAIDX.out.versions)
     }
     else if (fasta_fai_in) {
         fasta_fai = channel.fromPath(fasta_fai_in).map { fai -> [[id: 'fai'], fai] }.collect()
diff --git a/tests/aligner-bwa-mem.nf.test.snap b/tests/aligner-bwa-mem.nf.test.snap
index 0ca5918bbd..f450b48c78 100644
--- a/tests/aligner-bwa-mem.nf.test.snap
+++ b/tests/aligner-bwa-mem.nf.test.snap
@@ -4,7 +4,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -20,10 +20,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -204,7 +204,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -220,10 +220,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/aligner-bwa-mem2.nf.test.snap b/tests/aligner-bwa-mem2.nf.test.snap
index da63c14f1b..5879c7efa9 100644
--- a/tests/aligner-bwa-mem2.nf.test.snap
+++ b/tests/aligner-bwa-mem2.nf.test.snap
@@ -67,7 +67,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM2_INDEX": {
                     "bwamem2": "2.2.1"
@@ -83,10 +83,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -204,7 +204,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM2_INDEX": {
                     "bwamem2": "2.2.1"
@@ -220,10 +220,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/aligner-dragmap.nf.test.snap b/tests/aligner-dragmap.nf.test.snap
index b59f4a72f0..5a1f1fb712 100644
--- a/tests/aligner-dragmap.nf.test.snap
+++ b/tests/aligner-dragmap.nf.test.snap
@@ -72,7 +72,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -89,10 +89,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -209,7 +209,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -226,10 +226,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index 5eba11e3e1..9efe301a64 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -7,7 +7,7 @@
                     "bwa": "0.7.19-r1273"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -19,19 +19,20 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
                     "parabricks": "4.6.0-1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -197,7 +198,7 @@
                 "mosdepth_perchrom.txt:md5,f3a4ba86603c5121a26f54616a39c1ba",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
-                "samtools-stats-dp.txt:md5,068d671d8e3fa0a12b9c36072fb36808",
+                "samtools-stats-dp.txt:md5,9dbdd996ce0e7edb5b7861eb13eba285",
                 "samtools_alignment_plot.txt:md5,d2729501a111a33268c008ef6f18c7cd",
                 "samtools_insert_size.txt:md5,1553c58beee5f377cddbcbe8c527720a",
                 "genome.fasta.amb:md5,1891c1de381b3a96d4e72f590fde20c1",
@@ -213,9 +214,9 @@
                 "test.recal.mosdepth.region.dist.txt:md5,45016e5a0b9afb065c56833ba66f3049",
                 "test.recal.mosdepth.summary.txt:md5,3968d28e14fd99350ec75e919778ad0f",
                 "test.recal.per-base.bed.gz:md5,ab3fb06c65b28f774b22973085699d51",
-                "test.recal.per-base.bed.gz.csi:md5,bc588fff78a04f4c31e16768ddde204d",
+                "test.recal.per-base.bed.gz.csi:md5,2171094832c1eab19654be30cf0386d8",
                 "test.recal.regions.bed.gz:md5,912777bfe53139edf4948106611d3aeb",
-                "test.recal.regions.bed.gz.csi:md5,e24653e1e1cc9703eecaee9ebf3d2b13"
+                "test.recal.regions.bed.gz.csi:md5,6f7ee623716b66db7f62de827cec1324"
             ],
             [
                 "test.bam:md5,9da56e1adc10f4fa65303441b8a5a328"
@@ -226,11 +227,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T11:40:10.237605568",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-01-30T16:47:31.914017014"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner parabricks --wes": {
         "content": [
@@ -249,19 +250,20 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
                     "parabricks": "4.6.0-1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -431,7 +433,7 @@
                 "mosdepth_perchrom.txt:md5,f3a4ba86603c5121a26f54616a39c1ba",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
-                "samtools-stats-dp.txt:md5,068d671d8e3fa0a12b9c36072fb36808",
+                "samtools-stats-dp.txt:md5,9dbdd996ce0e7edb5b7861eb13eba285",
                 "samtools_alignment_plot.txt:md5,d2729501a111a33268c008ef6f18c7cd",
                 "samtools_insert_size.txt:md5,1553c58beee5f377cddbcbe8c527720a",
                 "genome.fasta.amb:md5,1891c1de381b3a96d4e72f590fde20c1",
@@ -447,9 +449,9 @@
                 "test.recal.mosdepth.region.dist.txt:md5,45016e5a0b9afb065c56833ba66f3049",
                 "test.recal.mosdepth.summary.txt:md5,3968d28e14fd99350ec75e919778ad0f",
                 "test.recal.per-base.bed.gz:md5,ab3fb06c65b28f774b22973085699d51",
-                "test.recal.per-base.bed.gz.csi:md5,bc588fff78a04f4c31e16768ddde204d",
+                "test.recal.per-base.bed.gz.csi:md5,2171094832c1eab19654be30cf0386d8",
                 "test.recal.regions.bed.gz:md5,912777bfe53139edf4948106611d3aeb",
-                "test.recal.regions.bed.gz.csi:md5,e24653e1e1cc9703eecaee9ebf3d2b13"
+                "test.recal.regions.bed.gz.csi:md5,6f7ee623716b66db7f62de827cec1324"
             ],
             "No BAM files",
             [
@@ -462,11 +464,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T11:40:10.53055388",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-01-30T16:54:59.62879975"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner parabricks --tools mutect2 --wes --input fastq_pair.csv": {
         "content": [
@@ -500,13 +502,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "LEARNREADORIENTATIONMODEL": {
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
@@ -515,7 +518,7 @@
                     "parabricks": "4.6.0-1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -741,7 +744,7 @@
                 "mosdepth_perchrom.txt:md5,8973b4a63f05d9443da33ccd1fd48cb1",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
                 "multiqc_fastqc.txt:md5,2fd25e8c81f962594b801d5a9df3cd87",
-                "samtools-stats-dp.txt:md5,a2f978ab572ed3c72e3af6e605708d59",
+                "samtools-stats-dp.txt:md5,af97f6426bc8c6b9d812cc97e880743f",
                 "samtools_alignment_plot.txt:md5,94d6398ce03eefb42aa6f106c8d36e89",
                 "samtools_insert_size.txt:md5,3cf6a33d6676f31b5433f2cc4bf887e0",
                 "genome.fasta.amb:md5,1891c1de381b3a96d4e72f590fde20c1",
@@ -758,16 +761,16 @@
                 "test.recal.mosdepth.region.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.recal.mosdepth.summary.txt:md5,2ea3534987f28b3ed0b64a8e7986b442",
                 "test.recal.per-base.bed.gz:md5,e04122db11a66ad6ef7c851511b1b505",
-                "test.recal.per-base.bed.gz.csi:md5,a0c543664c7e5902d1cb56c25f4123b3",
+                "test.recal.per-base.bed.gz.csi:md5,a815256bdb29a623bb11677e4fbc69e3",
                 "test.recal.regions.bed.gz:md5,339335cc6d0a8048a3e328eaa93c5160",
-                "test.recal.regions.bed.gz.csi:md5,a43bfb8c598e3091406b25ba174c0270",
+                "test.recal.regions.bed.gz.csi:md5,cbc8b8b2bad75646406113dbc8cd13b8",
                 "test2.recal.mosdepth.global.dist.txt:md5,f25166c3a0051bb4d8c11a210278de6c",
                 "test2.recal.mosdepth.region.dist.txt:md5,f25166c3a0051bb4d8c11a210278de6c",
                 "test2.recal.mosdepth.summary.txt:md5,325213ef7bbb89b9ae27ef59252eca49",
                 "test2.recal.per-base.bed.gz:md5,027f5d98da0e283df866f2d9ac8255db",
-                "test2.recal.per-base.bed.gz.csi:md5,c471e17d82fddaeca77167331dead581",
+                "test2.recal.per-base.bed.gz.csi:md5,8f1b503a7653f4637dd83bca4f7dccca",
                 "test2.recal.regions.bed.gz:md5,7f23663b3565fb9546dfaa829dd68c61",
-                "test2.recal.regions.bed.gz.csi:md5,e24653e1e1cc9703eecaee9ebf3d2b13",
+                "test2.recal.regions.bed.gz.csi:md5,6f7ee623716b66db7f62de827cec1324",
                 "test2_vs_test.mutect2.filtered.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "test2_vs_test.mutect2.filtered.TsTv.count:md5,8dcfdbcaac118df1d5ad407dd2af699f",
                 "test.mutect2.pileups.table:md5,4a89cfa663f1552aeb80d5c31aa36e3d",
@@ -792,11 +795,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T11:45:10.811937172",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-30T13:32:40.97607962"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner parabricks --wes --intervals": {
         "content": [
@@ -812,19 +815,20 @@
                     "fastqc": "0.12.1"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
                     "parabricks": "4.6.0-1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -993,7 +997,7 @@
                 "mosdepth_perchrom.txt:md5,63cfbfc1ea970a26a7f0470efd9ad692",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
                 "multiqc_fastqc.txt:md5,bde0d0bffa62228b33fb68b7e25b6ff8",
-                "samtools-stats-dp.txt:md5,068d671d8e3fa0a12b9c36072fb36808",
+                "samtools-stats-dp.txt:md5,9dbdd996ce0e7edb5b7861eb13eba285",
                 "samtools_alignment_plot.txt:md5,d2729501a111a33268c008ef6f18c7cd",
                 "samtools_insert_size.txt:md5,1553c58beee5f377cddbcbe8c527720a",
                 "genome.fasta.amb:md5,1891c1de381b3a96d4e72f590fde20c1",
@@ -1008,9 +1012,9 @@
                 "test.recal.mosdepth.region.dist.txt:md5,a9e42dc9d21073821858d40ffce5bcde",
                 "test.recal.mosdepth.summary.txt:md5,ac93e4aa5a5229d001404513816cc219",
                 "test.recal.per-base.bed.gz:md5,ab3fb06c65b28f774b22973085699d51",
-                "test.recal.per-base.bed.gz.csi:md5,bc588fff78a04f4c31e16768ddde204d",
+                "test.recal.per-base.bed.gz.csi:md5,2171094832c1eab19654be30cf0386d8",
                 "test.recal.regions.bed.gz:md5,326c62bcdb340f213cc4514213516415",
-                "test.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309"
+                "test.recal.regions.bed.gz.csi:md5,ae98c5e0c344c0961bc9273e26fcb803"
             ],
             "No BAM files",
             [
@@ -1023,10 +1027,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T11:44:05.461520368",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-01-30T16:51:33.339994069"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/alignment_from_everything.nf.test.snap b/tests/alignment_from_everything.nf.test.snap
index 14e2c4a9d6..0e37ebd638 100644
--- a/tests/alignment_from_everything.nf.test.snap
+++ b/tests/alignment_from_everything.nf.test.snap
@@ -14,10 +14,10 @@
                     "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "COLLATE_FASTQ_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -39,28 +39,29 @@
                     "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MERGE_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SPRING_DECOMPRESS_TO_FQ_PAIR": {
                     "spring": "1.1.1"
@@ -372,49 +373,49 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,63dfeeafbef0aa87f48ab923402dcaed",
+                "samtools-stats-dp.txt:md5,6ff529835252acfb12982e1e749a12ab",
                 "samtools_alignment_plot.txt:md5,7c53a04c42499c92735d947bc8417dd6",
                 "samtools_insert_size.txt:md5,5a1a7d2501dbb68ff13f4b7e79b63e8c",
                 "test.md.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.md.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.md.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.md.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test.recal.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.recal.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.recal.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.recal.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.recal.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.recal.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test2.md.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test2.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test2.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test2.md.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test2.md.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test2.md.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test2.recal.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test2.recal.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test2.recal.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test2.recal.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test2.recal.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test2.recal.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test3.md.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test3.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test3.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test3.md.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test3.md.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test3.md.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test3.recal.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test3.recal.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test3.recal.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test3.recal.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test3.recal.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test3.recal.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test_bam.md.mosdepth.global.dist.txt:md5,9cb9b181119256ed17a77dcf44d58285",
                 "test_bam.md.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test_bam.md.mosdepth.summary.txt:md5,dbe376360e437c89190139ef0ae6769a",
                 "test_bam.md.regions.bed.gz:md5,0e5ed846b9b11717e42e93eec60f4ffc",
-                "test_bam.md.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537",
+                "test_bam.md.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test_bam.recal.mosdepth.global.dist.txt:md5,9cb9b181119256ed17a77dcf44d58285",
                 "test_bam.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test_bam.recal.mosdepth.summary.txt:md5,dbe376360e437c89190139ef0ae6769a",
                 "test_bam.recal.regions.bed.gz:md5,0e5ed846b9b11717e42e93eec60f4ffc",
-                "test_bam.recal.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537"
+                "test_bam.recal.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0"
             ],
             [
                 "test.sorted.bam:md5,59ecc5c82c7af1283eea7507c590c831",
@@ -434,7 +435,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-04-09T13:11:35.435247726",
+        "timestamp": "2026-07-27T10:23:15.832931857",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/alignment_to_fastq.nf.test.snap b/tests/alignment_to_fastq.nf.test.snap
index 7511faf85a..a2a82b769a 100644
--- a/tests/alignment_to_fastq.nf.test.snap
+++ b/tests/alignment_to_fastq.nf.test.snap
@@ -14,10 +14,10 @@
                     "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "COLLATE_FASTQ_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -39,28 +39,29 @@
                     "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MERGE_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -262,19 +263,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ea167e1f03920b6214de041740ca3cd5",
+                "samtools-stats-dp.txt:md5,55f72382d2fb37e9b0d8b5cc5de5f147",
                 "samtools_alignment_plot.txt:md5,7a727168e9bbb3f4c39894933438df98",
                 "samtools_insert_size.txt:md5,c00ec0f338c40f2f1bc129f46ffb9084",
                 "test.md.mosdepth.global.dist.txt:md5,9cb9b181119256ed17a77dcf44d58285",
                 "test.md.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.md.mosdepth.summary.txt:md5,dbe376360e437c89190139ef0ae6769a",
                 "test.md.regions.bed.gz:md5,0e5ed846b9b11717e42e93eec60f4ffc",
-                "test.md.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537",
+                "test.md.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test.recal.mosdepth.global.dist.txt:md5,9cb9b181119256ed17a77dcf44d58285",
                 "test.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.recal.mosdepth.summary.txt:md5,dbe376360e437c89190139ef0ae6769a",
                 "test.recal.regions.bed.gz:md5,0e5ed846b9b11717e42e93eec60f4ffc",
-                "test.recal.regions.bed.gz.csi:md5,d0713716f63ac573f4a3385733e9a537"
+                "test.recal.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0"
             ],
             [
                 "test.sorted.bam:md5,6934a96fff1eaa2f70b31ab7e11d3598",
@@ -285,10 +286,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:21:40.510324699",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T20:13:07.668460853"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index 7a68e41943..f214e1b69d 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -39,13 +39,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -363,10 +364,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-05-19T17:32:09.176203172",
+        "timestamp": "2026-07-27T10:22:41.864255495",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools bbsplit,strelka --bbsplit_fasta_list -stub": {
@@ -409,13 +410,13 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -636,13 +637,14 @@
                     "samtools": "1.21"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_FAIDX": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -836,10 +838,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-02-06T14:28:35.628964819",
+        "timestamp": "2026-07-27T10:25:04.052795149",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index c2f891429b..ab53f6469e 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -33,13 +33,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -296,7 +297,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
+                "samtools-stats-dp.txt:md5,92dc10e61df31bf4286b16582bed488f",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
                 "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
@@ -304,12 +305,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.md.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.md.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.md.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.recal.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.recal.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.recal.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.recal.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.recal.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.recal.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.strelka.variants.FILTER.summary:md5,dd87f507da7de20d5318841af312493b",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -326,10 +327,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-05-19T18:36:49.982943659",
+        "timestamp": "2026-07-27T10:25:08.849820282",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test -stub": {
@@ -366,13 +367,13 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
diff --git a/tests/fastp.nf.test.snap b/tests/fastp.nf.test.snap
index 111b6110ba..9d642ce582 100644
--- a/tests/fastp.nf.test.snap
+++ b/tests/fastp.nf.test.snap
@@ -33,13 +33,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -326,7 +327,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,58ea8c88565a359af678aa588e44895c",
+                "samtools-stats-dp.txt:md5,3f430eb99682ee0d3960a42c5a43650e",
                 "samtools_alignment_plot.txt:md5,aac925e42c9da438ab9f0fb7ae77e2fa",
                 "samtools_insert_size.txt:md5,9eccc96ce006c0161497782ed3afc147",
                 "test-test_L1_R1.fastp.fastq.gz:md5,f1a5c524cae7be9b5ca9a4138f847cfa",
@@ -339,12 +340,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,02d51752367e753a6984c12f059499ba",
                 "test.md.mosdepth.summary.txt:md5,f18e776c3ee8e6947c3a69c136f54860",
                 "test.md.regions.bed.gz:md5,a3e8ccd3f04d3aee009a109d52b69920",
-                "test.md.regions.bed.gz.csi:md5,74d39fd1e463ce7b77610c96f5f57daf",
+                "test.md.regions.bed.gz.csi:md5,4b8894bcd39f647bff72e55fe6b5fe4d",
                 "test.recal.mosdepth.global.dist.txt:md5,b1c26e3381f220e65d683048ab6b6e2a",
                 "test.recal.mosdepth.region.dist.txt:md5,02d51752367e753a6984c12f059499ba",
                 "test.recal.mosdepth.summary.txt:md5,f18e776c3ee8e6947c3a69c136f54860",
                 "test.recal.regions.bed.gz:md5,a3e8ccd3f04d3aee009a109d52b69920",
-                "test.recal.regions.bed.gz.csi:md5,74d39fd1e463ce7b77610c96f5f57daf"
+                "test.recal.regions.bed.gz.csi:md5,4b8894bcd39f647bff72e55fe6b5fe4d"
             ],
             "No BAM files",
             [
@@ -356,11 +357,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:23:58.917345607",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-17T14:34:45.9737357"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --split_fastq 150000": {
         "content": [
@@ -396,13 +397,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -701,7 +703,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,93ec69f6db74d9de7e4a549491ae2c73",
+                "samtools-stats-dp.txt:md5,75006287063bdff5824a9ccc46567138",
                 "samtools_alignment_plot.txt:md5,962b7dd27f48ec4bbb668c8c32cbea1b",
                 "samtools_insert_size.txt:md5,555c4ca8aac120c3b23e6cd2457922cd",
                 "0001.test-test_L1_R1.fastp.fastq.gz:md5,f379a60cc2a41f39fa0c0d23edf6eb61",
@@ -726,12 +728,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,5062f8b7bb536c9b77a68f4ccd2315c2",
                 "test.md.mosdepth.summary.txt:md5,455358d5943fd1e5f09853acff3e50b6",
                 "test.md.regions.bed.gz:md5,729091bfb7c08486c8d247f4629996e6",
-                "test.md.regions.bed.gz.csi:md5,295eb71f59c0e37b5c3cf289a467f97e",
+                "test.md.regions.bed.gz.csi:md5,fc3378906815c0591f36b0e2d9d6fad8",
                 "test.recal.mosdepth.global.dist.txt:md5,e5d0c6bf323c32f5414bd48b90bb32fa",
                 "test.recal.mosdepth.region.dist.txt:md5,5062f8b7bb536c9b77a68f4ccd2315c2",
                 "test.recal.mosdepth.summary.txt:md5,455358d5943fd1e5f09853acff3e50b6",
                 "test.recal.regions.bed.gz:md5,729091bfb7c08486c8d247f4629996e6",
-                "test.recal.regions.bed.gz.csi:md5,295eb71f59c0e37b5c3cf289a467f97e"
+                "test.recal.regions.bed.gz.csi:md5,fc3378906815c0591f36b0e2d9d6fad8"
             ],
             "No BAM files",
             [
@@ -743,11 +745,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:21:50.402269939",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-06T15:10:32.391037"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --trim_fastq (no save)": {
         "content": [
@@ -783,13 +785,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1070,7 +1073,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
+                "samtools-stats-dp.txt:md5,92dc10e61df31bf4286b16582bed488f",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
                 "test-test_L1.fastp.json:md5,a7746b05c3ffb374c8f5d204f971879e",
@@ -1079,12 +1082,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.md.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.md.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.md.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.recal.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.recal.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.recal.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.recal.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.recal.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2"
+                "test.recal.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623"
             ],
             "No BAM files",
             [
@@ -1096,10 +1099,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:21:18.402385401",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-06T15:02:50.499879"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
\ No newline at end of file
diff --git a/tests/intervals.nf.test.snap b/tests/intervals.nf.test.snap
index d4af6db508..132408824a 100644
--- a/tests/intervals.nf.test.snap
+++ b/tests/intervals.nf.test.snap
@@ -4,7 +4,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BUILD_INTERVALS": {
                     "gawk": "5.3.1"
@@ -20,10 +20,10 @@
                     "gawk": "5.3.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -88,7 +88,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -101,10 +101,10 @@
                     "gawk": "5.3.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -150,7 +150,7 @@
             7,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -160,10 +160,10 @@
                     "samtools": "1.22.1"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -208,7 +208,7 @@
             7,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -218,10 +218,10 @@
                     "samtools": "1.22.1"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -261,7 +261,7 @@
             9,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -274,10 +274,10 @@
                     "gawk": "5.3.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -318,7 +318,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BUILD_INTERVALS": {
                     "gawk": "5.3.1"
@@ -334,10 +334,10 @@
                     "gawk": "5.3.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/joint_calling_haplotypecaller.nf.test.snap b/tests/joint_calling_haplotypecaller.nf.test.snap
index dd4c356dfe..d6cf847b4b 100644
--- a/tests/joint_calling_haplotypecaller.nf.test.snap
+++ b/tests/joint_calling_haplotypecaller.nf.test.snap
@@ -28,10 +28,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -196,7 +197,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,46e5004d6eb0d4f451706dcbb7acc2ed",
                 "mosdepth_perchrom.txt:md5,a9d61b7a7c54a27857b03a1c51ba6ff5",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "joint_germline.bcftools_stats.txt:md5,8d7de0e9bbf007cf6c80caaa5e6e9ea2",
@@ -204,16 +205,16 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "testN.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
                 "testN.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "testN.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "testN.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "testN.recal.regions.bed.gz:md5,0c8215fbea7b0bf7aba9d1781575f905",
-                "testN.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "testN.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "testT.recal.mosdepth.global.dist.txt:md5,ba97ed85645f77da6f3adad138b3cdb4",
                 "testT.recal.mosdepth.region.dist.txt:md5,a7eb835371dd0aaf347ccca7ebe1eb3b",
                 "testT.recal.mosdepth.summary.txt:md5,a937108cbf24c1430b79c861234ce22b",
                 "testT.recal.per-base.bed.gz:md5,fde70b7a0caef4460692540b97b3fd52",
-                "testT.recal.per-base.bed.gz.csi:md5,7f62d96cdff1ce2acc0c7d2d0549cb1b",
+                "testT.recal.per-base.bed.gz.csi:md5,ff9ad8863871d40f0f0ef283a74fdae2",
                 "testT.recal.regions.bed.gz:md5,8f0d545a0950c6a53225abec38553f6f",
-                "testT.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "testT.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "joint_germline.FILTER.summary:md5,2a4eb7abfb2e64e45d53fdda17530b7f",
                 "joint_germline.TsTv.count:md5,949fa16c755189c23a37f0ea8ecd1b26"
             ],
@@ -228,11 +229,11 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
+        "timestamp": "2026-07-27T10:17:46.812394654",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T19:58:09.342469278"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "Fails with --no_intervals and --joint_germline for haplotypecaller": {
         "content": [
@@ -280,10 +281,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -448,7 +450,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,46e5004d6eb0d4f451706dcbb7acc2ed",
                 "mosdepth_perchrom.txt:md5,a9d61b7a7c54a27857b03a1c51ba6ff5",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "joint_germline.bcftools_stats.txt:md5,8d7de0e9bbf007cf6c80caaa5e6e9ea2",
@@ -456,16 +458,16 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "testN.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
                 "testN.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "testN.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "testN.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "testN.recal.regions.bed.gz:md5,0c8215fbea7b0bf7aba9d1781575f905",
-                "testN.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "testN.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "testT.recal.mosdepth.global.dist.txt:md5,ba97ed85645f77da6f3adad138b3cdb4",
                 "testT.recal.mosdepth.region.dist.txt:md5,a7eb835371dd0aaf347ccca7ebe1eb3b",
                 "testT.recal.mosdepth.summary.txt:md5,a937108cbf24c1430b79c861234ce22b",
                 "testT.recal.per-base.bed.gz:md5,fde70b7a0caef4460692540b97b3fd52",
-                "testT.recal.per-base.bed.gz.csi:md5,7f62d96cdff1ce2acc0c7d2d0549cb1b",
+                "testT.recal.per-base.bed.gz.csi:md5,ff9ad8863871d40f0f0ef283a74fdae2",
                 "testT.recal.regions.bed.gz:md5,8f0d545a0950c6a53225abec38553f6f",
-                "testT.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "testT.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "joint_germline.FILTER.summary:md5,2a4eb7abfb2e64e45d53fdda17530b7f",
                 "joint_germline.TsTv.count:md5,949fa16c755189c23a37f0ea8ecd1b26"
             ],
@@ -480,10 +482,10 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
+        "timestamp": "2026-07-27T10:19:45.167839338",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T19:59:28.831246669"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 2ecca9bc76..88da7e4892 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -16,13 +16,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -191,7 +192,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f08ab9b4898020d43b2e0ff77212f5b2",
                 "mosdepth_perchrom.txt:md5,05b840db853703d42c6c0be360cb573e",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,fe7c4ec200c94df7ff97a0e8cbec2f70",
+                "samtools-stats-dp.txt:md5,801a1756a694e97039aae8500a5477f6",
                 "samtools_alignment_plot.txt:md5,82d6fb277fbbbd221a92ebda4567d9d9",
                 "samtools_insert_size.txt:md5,00660bb059693565c1189ce98356e414",
                 "test.mutect2.bcftools_stats.txt:md5,2de4f9f8da53b202e9d784b4e95c3245",
@@ -199,23 +200,23 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample1.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample1.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample1.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample1.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample1.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample1.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample1.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample2.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample2.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample3.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample3.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample3.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample3.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample3.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample3.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample3.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample3.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "test.mutect2.FILTER.summary:md5,19fd44ff1fbdfe422473bf1f5dc9cdb3",
                 "test.mutect2.TsTv.count:md5,aa51bde6080c015c6aa6c8254977dd11",
                 "test.mutect2.vcf.gz.stats:md5,6340205d9cc91fc3c2d243fc151c210b"
@@ -230,11 +231,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:33:51.065452986",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T20:17:32.428293264"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/recalibrated_tumoronly_joint.csv --tools mutect2 --joint_mutect2": {
         "content": [
@@ -256,13 +257,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -421,7 +423,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0bd6d5120cacef0c90192267731031a5",
                 "mosdepth_perchrom.txt:md5,a14350b5c82288d538ea43992a65be99",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e6019a625137ced6d426a503cd652559",
+                "samtools-stats-dp.txt:md5,32150435d1e8f1c6f47ba618a71f1e86",
                 "samtools_alignment_plot.txt:md5,cb01fb538637d281195e46e557031c7a",
                 "samtools_insert_size.txt:md5,97989575c42dadc81f347d661faa09cb",
                 "test.mutect2.bcftools_stats.txt:md5,3079129e89fdaf04a3403d888a99fd81",
@@ -429,16 +431,16 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,b0b47739dcafeeb1a9e6218b8abca1e0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,fb0efeba20ea272b7b709cf65246689e",
-                "sample2.recal.regions.bed.gz.csi:md5,e8452848671e9e5c147ff4cceee944af",
+                "sample2.recal.regions.bed.gz.csi:md5,e95efa0e55e9f9699b48e6a661d5e1e4",
                 "sample3.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample3.recal.mosdepth.region.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample3.recal.mosdepth.summary.txt:md5,b0b47739dcafeeb1a9e6218b8abca1e0",
                 "sample3.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample3.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample3.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample3.recal.regions.bed.gz:md5,fb0efeba20ea272b7b709cf65246689e",
-                "sample3.recal.regions.bed.gz.csi:md5,e8452848671e9e5c147ff4cceee944af",
+                "sample3.recal.regions.bed.gz.csi:md5,e95efa0e55e9f9699b48e6a661d5e1e4",
                 "test.mutect2.FILTER.summary:md5,b55562501b24e3cec53c22ffe0ce346f",
                 "test.mutect2.TsTv.count:md5,d06a480e205f2f894db2bb51846b4c39",
                 "test.mutect2.vcf.gz.stats:md5,bd9ff0f343dd60fdd2860fdeff617a51"
@@ -453,10 +455,10 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:32:24.759023585",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T20:42:22.769087844"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/lane_integer.nf.test.snap b/tests/lane_integer.nf.test.snap
index e6d0a7f91a..b2462b19ff 100644
--- a/tests/lane_integer.nf.test.snap
+++ b/tests/lane_integer.nf.test.snap
@@ -4,7 +4,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -20,10 +20,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/multi_lane.nf.test.snap b/tests/multi_lane.nf.test.snap
index 334e9fbdbe..4a53664d79 100644
--- a/tests/multi_lane.nf.test.snap
+++ b/tests/multi_lane.nf.test.snap
@@ -4,7 +4,7 @@
             21,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -30,13 +30,13 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -119,10 +119,11 @@
             25,
             {
                 "BAM2FASTQ": {
-                    "samtools": 1.21
+                    "bgzip": "1.24",
+                    "samtools": "1.24"
                 },
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -138,10 +139,10 @@
                     "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "COLLATE_FASTQ_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -156,25 +157,25 @@
                     "fgbio": "3.1.2"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CONSENSUS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_MERGE_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -222,10 +223,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_umi_cleanname_1.fastq.gz): Cannot extract flowcell ID from @922332"
             ]
         ],
+        "timestamp": "2026-07-27T10:26:26.437520414",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-11T11:53:37.119233"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
\ No newline at end of file
diff --git a/tests/postprocess_concatenation.nf.test.snap b/tests/postprocess_concatenation.nf.test.snap
index 9eb4b11103..6874962f23 100644
--- a/tests/postprocess_concatenation.nf.test.snap
+++ b/tests/postprocess_concatenation.nf.test.snap
@@ -28,10 +28,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -247,7 +248,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
                 "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
@@ -258,12 +259,12 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "testN.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
                 "testT.recal.mosdepth.region.dist.txt:md5,ccf646922b05cb4759c4f89072be2b69",
                 "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
                 "testT.recal.regions.bed.gz:md5,14b36a2cf428840aab471f95cfbe399f",
-                "testT.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testN.freebayes.filtered.FILTER.summary:md5,e2f8e86fb18631c0bf6ccd7d9d4039c8",
                 "testN.freebayes.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc",
                 "testT.freebayes.filtered.FILTER.summary:md5,4b25443c427fd27761017e4f3a556b29",
@@ -295,10 +296,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:26:19.867067032",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-03T11:06:26.54547"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/postprocess_concatenation_normalization.nf.test.snap b/tests/postprocess_concatenation_normalization.nf.test.snap
index 3c4a7ecd72..fe3dcb3881 100644
--- a/tests/postprocess_concatenation_normalization.nf.test.snap
+++ b/tests/postprocess_concatenation_normalization.nf.test.snap
@@ -31,10 +31,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -278,7 +279,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
                 "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
@@ -289,12 +290,12 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "testN.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
                 "testT.recal.mosdepth.region.dist.txt:md5,ccf646922b05cb4759c4f89072be2b69",
                 "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
                 "testT.recal.regions.bed.gz:md5,14b36a2cf428840aab471f95cfbe399f",
-                "testT.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testN.freebayes.filtered.FILTER.summary:md5,e2f8e86fb18631c0bf6ccd7d9d4039c8",
                 "testN.freebayes.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc",
                 "testT.freebayes.filtered.FILTER.summary:md5,4b25443c427fd27761017e4f3a556b29",
@@ -330,11 +331,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:22:40.767625875",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-03T10:48:21.505593"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --normalize_vcfs --concatenate_vcfs --tools freebayes,strelka": {
         "content": [
@@ -365,10 +366,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -601,7 +603,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
                 "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
@@ -612,12 +614,12 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "testN.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
                 "testT.recal.mosdepth.region.dist.txt:md5,ccf646922b05cb4759c4f89072be2b69",
                 "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
                 "testT.recal.regions.bed.gz:md5,14b36a2cf428840aab471f95cfbe399f",
-                "testT.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testN.freebayes.filtered.FILTER.summary:md5,e2f8e86fb18631c0bf6ccd7d9d4039c8",
                 "testN.freebayes.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc",
                 "testT.freebayes.filtered.FILTER.summary:md5,4b25443c427fd27761017e4f3a556b29",
@@ -653,10 +655,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:25:23.340652566",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-03T10:44:17.917186"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/postprocess_consensus.nf.test.snap b/tests/postprocess_consensus.nf.test.snap
index 4273d70f6d..dc5a270f5d 100644
--- a/tests/postprocess_consensus.nf.test.snap
+++ b/tests/postprocess_consensus.nf.test.snap
@@ -35,13 +35,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -307,7 +308,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.bcftools.bcftools_stats.txt:md5,bb4018e2ec62ab957867c4f55ce626b8",
@@ -319,16 +320,16 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample3.bcftools.FILTER.summary:md5,9b62595b026decf12e9198d531e4307a",
                 "sample3.bcftools.TsTv.count:md5,6c937125d7bac4c491bea50f18cba43a",
                 "sample4_vs_sample3.mutect2.FILTER.summary:md5,cac64448be577632a614af62a23af34a",
@@ -371,11 +372,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:23:15.482817797",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T14:58:25.361891009"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --snv_consensus_calling --normalize_vcfs --tools lofreq,mpileup,mutect2 (tumor-only)": {
         "content": [
@@ -410,13 +411,14 @@
                     "lofreq": "2.1.5"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -622,7 +624,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,1036ea76acae803f591fd99838a8eded",
                 "mosdepth_perchrom.txt:md5,1f24f2f40467234c410a8bda544a8aae",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.bcftools.bcftools_stats.txt:md5,47ce340432efdbb38a86d8b7c3d0c2d4",
@@ -632,9 +634,9 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample2.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample2.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample2.bcftools.FILTER.summary:md5,8766995f3e4119ef30dfdaa9fb3752ce",
                 "sample2.bcftools.TsTv.count:md5,01df95fcb4df593f7e1b214d90ebdb59",
                 "sample2.lofreq.FILTER.summary:md5,8dd8a0c91d5c4a260b462e04f615e502",
@@ -688,11 +690,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:22:11.362506122",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T15:02:50.699837449"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --snv_consensus_calling --normalize_vcfs --tools mpileup,mutect2,strelka (germline + somatic)": {
         "content": [
@@ -739,13 +741,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -1007,7 +1010,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.bcftools.bcftools_stats.txt:md5,bb4018e2ec62ab957867c4f55ce626b8",
@@ -1019,16 +1022,16 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample3.bcftools.FILTER.summary:md5,9b62595b026decf12e9198d531e4307a",
                 "sample3.bcftools.TsTv.count:md5,6c937125d7bac4c491bea50f18cba43a",
                 "sample4_vs_sample3.mutect2.filtered.FILTER.summary:md5,b25d4d2a64f9590d0ffb119fd3adb06e",
@@ -1119,10 +1122,10 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:27:08.769937074",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-06T14:53:33.998324217"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/postprocess_filtering.nf.test.snap b/tests/postprocess_filtering.nf.test.snap
index b985b4bbda..31b423c532 100644
--- a/tests/postprocess_filtering.nf.test.snap
+++ b/tests/postprocess_filtering.nf.test.snap
@@ -22,10 +22,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -241,7 +242,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
                 "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
@@ -252,12 +253,12 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "testN.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
                 "testT.recal.mosdepth.region.dist.txt:md5,ccf646922b05cb4759c4f89072be2b69",
                 "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
                 "testT.recal.regions.bed.gz:md5,14b36a2cf428840aab471f95cfbe399f",
-                "testT.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testN.freebayes.filtered.FILTER.summary:md5,e2f8e86fb18631c0bf6ccd7d9d4039c8",
                 "testN.freebayes.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc",
                 "testT.freebayes.filtered.FILTER.summary:md5,4b25443c427fd27761017e4f3a556b29",
@@ -291,10 +292,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:19:51.840489661",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-03T11:32:58.31191"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/postprocess_normalization.nf.test.snap b/tests/postprocess_normalization.nf.test.snap
index 6465eb8a6e..42f044a65a 100644
--- a/tests/postprocess_normalization.nf.test.snap
+++ b/tests/postprocess_normalization.nf.test.snap
@@ -22,10 +22,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -251,7 +252,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
                 "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,a0c101497a0566f60b9e8388207944ab",
+                "samtools-stats-dp.txt:md5,1f1da592b5ef6dc0339d4d0b7b9aa03c",
                 "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
                 "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
                 "testN.freebayes.filtered.bcftools_stats.txt:md5,2429f8a81b14bcc4ac07fccd75c2f837",
@@ -262,12 +263,12 @@
                 "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "testN.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
                 "testT.recal.mosdepth.region.dist.txt:md5,ccf646922b05cb4759c4f89072be2b69",
                 "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
                 "testT.recal.regions.bed.gz:md5,14b36a2cf428840aab471f95cfbe399f",
-                "testT.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "testN.freebayes.filtered.FILTER.summary:md5,e2f8e86fb18631c0bf6ccd7d9d4039c8",
                 "testN.freebayes.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc",
                 "testT.freebayes.filtered.FILTER.summary:md5,4b25443c427fd27761017e4f3a556b29",
@@ -303,10 +304,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:18:59.909328969",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-03T11:48:18.652411"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index 30076c3c58..1d9259b618 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -43,7 +43,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "PREPROCESS_NORMAL": {
                     "varlociraptor": "8.9.5"
@@ -55,7 +56,7 @@
                     "rbt": "0.42.2"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SORT_CALLED_CHUNKS": {
                     "bcftools": "1.23.1"
@@ -277,7 +278,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
@@ -287,16 +288,16 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample3.strelka.variants.FILTER.summary:md5,2048a5de0201a6052c988a0189979a5f",
                 "sample3.strelka.variants.TsTv.count:md5,c5b7a8eda2526d899098439ae4c06a49",
                 "sample4_vs_sample3.strelka.somatic_indels.FILTER.summary:md5,3441628cd6550ed459ca1c3db989ceea",
@@ -322,11 +323,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:34:10.078345824",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T14:56:17.705455093"
+        }
     },
     "-profile test --tools mutect2,varlociraptor --input recalibrated_tumoronly.csv": {
         "content": [
@@ -369,7 +370,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
@@ -378,7 +380,7 @@
                     "rbt": "0.42.2"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SORT_CALLED_CHUNKS": {
                     "bcftools": "1.23.1"
@@ -557,7 +559,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,1036ea76acae803f591fd99838a8eded",
                 "mosdepth_perchrom.txt:md5,1f24f2f40467234c410a8bda544a8aae",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.mutect2.filtered.bcftools_stats.txt:md5,30c32a41d08f8ae7227521beae37dc62",
@@ -565,9 +567,9 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample2.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample2.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample2.mutect2.filtered.FILTER.summary:md5,276c858391322083833a42e04fe3554d",
                 "sample2.mutect2.filtered.TsTv.count:md5,fe3ff1f0c2ead72f037552727438e00a",
                 "sample2.mutect2.contamination.table:md5,46c708c943b453da89a3da08acfdb2a7",
@@ -588,11 +590,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:38:20.44515837",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T15:01:23.802458652"
+        }
     },
     "-profile test --tools strelka,varlociraptor --input recalibrated_germline.csv": {
         "content": [
@@ -620,13 +622,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "RBT_VCFSPLIT": {
                     "rbt": "0.42.2"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SORT_CALLED_CHUNKS": {
                     "bcftools": "1.23.1"
@@ -804,7 +807,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,b7016944a8325bef2c0ed542246acfa9",
                 "mosdepth_perchrom.txt:md5,04d9eccc248633a38fb253bc70fb8d62",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
@@ -812,9 +815,9 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample1.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample1.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample1.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample1.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample1.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample1.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample1.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample1.strelka.variants.FILTER.summary:md5,2048a5de0201a6052c988a0189979a5f",
                 "sample1.strelka.variants.TsTv.count:md5,c5b7a8eda2526d899098439ae4c06a49",
                 "sample1.scenario.varlociraptor.yaml:md5,bdc995369d2dede79535e551530c0cf6"
@@ -830,10 +833,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:23:35.977627481",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T14:45:50.699233572"
+        }
     }
 }
\ No newline at end of file
diff --git a/tests/qc_ngscheckmate.nf.test.snap b/tests/qc_ngscheckmate.nf.test.snap
index bba16b79b9..44d732ff4b 100644
--- a/tests/qc_ngscheckmate.nf.test.snap
+++ b/tests/qc_ngscheckmate.nf.test.snap
@@ -16,7 +16,7 @@
                     "ngscheckmate": "1.0.1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -133,13 +133,14 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "NGSCHECKMATE_NCM": {
                     "ngscheckmate": "1.0.1"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -252,29 +253,29 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,ad0637d55d7025330f2f6cb7f9680e64",
                 "mosdepth_perchrom.txt:md5,73ef9a077df1887f9021a581fbf207bc",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
+                "samtools-stats-dp.txt:md5,a6501ad36c9d1e9b97878e69f072d63c",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "ngscheckmate_all.txt:md5,f858e3eb892b8245cbf74cc979e4f33a",
                 "ngscheckmate_matched.txt:md5,ab2c5b46e9a4dfb3bb54292db931b58b",
                 "ngscheckmate_output_corr_matrix.txt:md5,a86afff85677c875503d495cbbb4f495"
@@ -289,10 +290,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2025-09-30T21:27:36.837652254",
+        "timestamp": "2026-07-27T10:33:27.14434232",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/save_mapped.nf.test.snap b/tests/save_mapped.nf.test.snap
index 36e862f55e..8467fabb4a 100644
--- a/tests/save_mapped.nf.test.snap
+++ b/tests/save_mapped.nf.test.snap
@@ -4,7 +4,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -20,10 +20,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 52c8d0fe9d..491d13b64b 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -51,13 +51,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -330,7 +331,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
+                "samtools-stats-dp.txt:md5,92dc10e61df31bf4286b16582bed488f",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
                 "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
@@ -338,12 +339,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.md.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.md.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.md.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.recal.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.recal.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.recal.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.recal.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.recal.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.recal.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.strelka.variants.FILTER.summary:md5,dd87f507da7de20d5318841af312493b",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
@@ -372,11 +373,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:27:46.994496963",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-13T09:13:12.307067"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --save_output_as_bam skip QC/recal/md": {
         "content": [
@@ -396,10 +397,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/sentieon.nf.test.snap b/tests/sentieon.nf.test.snap
index 5387d5cdb8..a14708ec3f 100644
--- a/tests/sentieon.nf.test.snap
+++ b/tests/sentieon.nf.test.snap
@@ -26,13 +26,13 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
diff --git a/tests/sentieon_aligner_bwamem.nf.test.snap b/tests/sentieon_aligner_bwamem.nf.test.snap
index 48c7ef8233..516178e64a 100644
--- a/tests/sentieon_aligner_bwamem.nf.test.snap
+++ b/tests/sentieon_aligner_bwamem.nf.test.snap
@@ -4,7 +4,7 @@
             10,
             {
                 "BAM_TO_CRAM_MAPPING": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -16,10 +16,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_BWAMEM": {
                     "bwa": "0.7.17-r1188",
diff --git a/tests/sentieon_dedup.nf.test.snap b/tests/sentieon_dedup.nf.test.snap
index b2424d75a7..4c73072939 100644
--- a/tests/sentieon_dedup.nf.test.snap
+++ b/tests/sentieon_dedup.nf.test.snap
@@ -16,13 +16,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
@@ -163,19 +164,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,b20f475e03ef81a68b3dec99b7e6aae4",
+                "samtools-stats-dp.txt:md5,e1ea864eb9632fcb7bc548122bff5aac",
                 "samtools_alignment_plot.txt:md5,b693942009526869bd21cbc42d54f53c",
                 "samtools_insert_size.txt:md5,7e95fe3ffb2346827b9e63da4de60842",
                 "test.dedup.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.dedup.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.dedup.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.dedup.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.dedup.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "test.dedup.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.recal.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.recal.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -185,11 +186,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:35:39.37304294",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T10:54:13.719485962"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dedup --step markduplicates --input tests/csv/3.0/mapped_single_cram.csv --sentieon_consensus": {
         "content": [
@@ -208,13 +209,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
@@ -355,19 +357,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,b7a972304062dab47bf1ef592defa909",
+                "samtools-stats-dp.txt:md5,6d7ae257a6d8786b2d3ae875d57c2c66",
                 "samtools_alignment_plot.txt:md5,5c19e1b5d6980e773e9a182557ae20f8",
                 "samtools_insert_size.txt:md5,223fb9c7f62b54ffe8a820b09c21b89d",
                 "test.dedup.mosdepth.global.dist.txt:md5,cc38f46822141ac16b5025ac5d62d044",
                 "test.dedup.mosdepth.region.dist.txt:md5,86b78626070a52dbe36d952b85814921",
                 "test.dedup.mosdepth.summary.txt:md5,3cf3700ecd4ec08825ab33e8bcbc3206",
                 "test.dedup.regions.bed.gz:md5,ba570a03993dbc155da2e5bf8e42f62e",
-                "test.dedup.regions.bed.gz.csi:md5,10b7f8e9ac2ac27205f8bc827a186195",
+                "test.dedup.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,cc38f46822141ac16b5025ac5d62d044",
                 "test.recal.mosdepth.region.dist.txt:md5,86b78626070a52dbe36d952b85814921",
                 "test.recal.mosdepth.summary.txt:md5,3cf3700ecd4ec08825ab33e8bcbc3206",
                 "test.recal.regions.bed.gz:md5,ba570a03993dbc155da2e5bf8e42f62e",
-                "test.recal.regions.bed.gz.csi:md5,10b7f8e9ac2ac27205f8bc827a186195"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -377,11 +379,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:37:42.701914376",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-08-15T09:38:15.648926052"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dedup --step markduplicates --input tests/csv/3.0/mapped_single_cram.csv": {
         "content": [
@@ -400,13 +402,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
@@ -547,19 +550,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,b20f475e03ef81a68b3dec99b7e6aae4",
+                "samtools-stats-dp.txt:md5,e1ea864eb9632fcb7bc548122bff5aac",
                 "samtools_alignment_plot.txt:md5,b693942009526869bd21cbc42d54f53c",
                 "samtools_insert_size.txt:md5,7e95fe3ffb2346827b9e63da4de60842",
                 "test.dedup.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.dedup.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.dedup.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.dedup.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.dedup.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "test.dedup.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.recal.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.recal.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -569,11 +572,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:34:59.34095366",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T11:05:41.448189865"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dedup --step markduplicates --input tests/csv/3.0/bam_umi_header.csv --sentieon_consensus --umi_in_read_header": {
         "content": [
@@ -595,13 +598,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
@@ -742,19 +746,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,36c50f0e76e2cdb1476248b8bffa60f3",
+                "samtools-stats-dp.txt:md5,a0fd3c6744845e917395774d709b1e1d",
                 "samtools_alignment_plot.txt:md5,511a3eba2589bb6790a4cfcd2a9c0d6c",
                 "samtools_insert_size.txt:md5,28a3cafdf305cd8c610017d1da8af854",
                 "test.dedup.mosdepth.global.dist.txt:md5,822c1f9c009f5436fefcfc9f5f7e6ee7",
                 "test.dedup.mosdepth.region.dist.txt:md5,1395b75e88a291268b96ec38f63c033e",
                 "test.dedup.mosdepth.summary.txt:md5,17f1b050f76ba0bfc96cbd045b627cd4",
                 "test.dedup.regions.bed.gz:md5,175dba70847fa0f2e5a44a014fa91027",
-                "test.dedup.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.dedup.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test.recal.mosdepth.global.dist.txt:md5,822c1f9c009f5436fefcfc9f5f7e6ee7",
                 "test.recal.mosdepth.region.dist.txt:md5,1395b75e88a291268b96ec38f63c033e",
                 "test.recal.mosdepth.summary.txt:md5,17f1b050f76ba0bfc96cbd045b627cd4",
                 "test.recal.regions.bed.gz:md5,175dba70847fa0f2e5a44a014fa91027",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.recal.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0"
             ],
             "No BAM files",
             [
@@ -764,11 +768,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:36:17.840418937",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-08-15T10:02:22.395300103"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dedup --step markduplicates --input tests/csv/3.0/bam_umi_header.csv --umi_in_read_header": {
         "content": [
@@ -790,13 +794,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DEDUP": {
                     "sentieon": "202503.02"
@@ -937,19 +942,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,a416a2f4042ce91471192ee33bab23ad",
+                "samtools-stats-dp.txt:md5,005cfcc30b93dc9be53956fcdf502bc2",
                 "samtools_alignment_plot.txt:md5,cf28e9513f09390047f12f904114a50c",
                 "samtools_insert_size.txt:md5,4212a4d5afe29c9a16599ab14b6c7cd9",
                 "test.dedup.mosdepth.global.dist.txt:md5,71d52337c3c971849645655e3203f9e0",
                 "test.dedup.mosdepth.region.dist.txt:md5,d985616d0cb8d74ff6448601d9a03318",
                 "test.dedup.mosdepth.summary.txt:md5,c01b82249d58f2f2c0d7473c588ffaf4",
                 "test.dedup.regions.bed.gz:md5,b49899dadd40a3b54b0c43033d740aa8",
-                "test.dedup.regions.bed.gz.csi:md5,5c556fe6462be13681267bb28257b654",
+                "test.dedup.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,71d52337c3c971849645655e3203f9e0",
                 "test.recal.mosdepth.region.dist.txt:md5,d985616d0cb8d74ff6448601d9a03318",
                 "test.recal.mosdepth.summary.txt:md5,c01b82249d58f2f2c0d7473c588ffaf4",
                 "test.recal.regions.bed.gz:md5,b49899dadd40a3b54b0c43033d740aa8",
-                "test.recal.regions.bed.gz.csi:md5,5c556fe6462be13681267bb28257b654"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -959,10 +964,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:35:19.642136626",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-08-15T09:50:20.120218465"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/spark.nf.test.snap b/tests/spark.nf.test.snap
index 294289be2b..3c1cb4afba 100644
--- a/tests/spark.nf.test.snap
+++ b/tests/spark.nf.test.snap
@@ -32,16 +32,17 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "INDEX_MARKDUPLICATES": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -218,19 +219,19 @@
                 "mosdepth_perchrom.txt:md5,3a7533e81978d33159d27b18cd337565",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
                 "multiqc_fastqc.txt:md5,d54614f8b2de16511949dbbde8f38a5d",
-                "samtools-stats-dp.txt:md5,440993b962f6c557cccfa2974648e1ec",
+                "samtools-stats-dp.txt:md5,6e3695fb7582152c9de1b527e3578d25",
                 "samtools_alignment_plot.txt:md5,46e8824724863d243a01cc239a5eff15",
                 "samtools_insert_size.txt:md5,17c44517c58803f9c078f6d9b55aed00",
                 "test2.md.mosdepth.global.dist.txt:md5,85d38a74ce189b9110c57cd94bc26757",
                 "test2.md.mosdepth.region.dist.txt:md5,286d57b7d9b3a95ef18ab2eb7f913d81",
                 "test2.md.mosdepth.summary.txt:md5,04b69ef7f00199dcea7822a79d2c7bd7",
                 "test2.md.regions.bed.gz:md5,292e177aa997597f83dc4e84bcc36b4c",
-                "test2.md.regions.bed.gz.csi:md5,5bf5fc178e4faf2462427502c3666004",
+                "test2.md.regions.bed.gz.csi:md5,2377c1d5f323117f03d96b4e83cd25cd",
                 "test2.recal.mosdepth.global.dist.txt:md5,85d38a74ce189b9110c57cd94bc26757",
                 "test2.recal.mosdepth.region.dist.txt:md5,286d57b7d9b3a95ef18ab2eb7f913d81",
                 "test2.recal.mosdepth.summary.txt:md5,04b69ef7f00199dcea7822a79d2c7bd7",
                 "test2.recal.regions.bed.gz:md5,292e177aa997597f83dc4e84bcc36b4c",
-                "test2.recal.regions.bed.gz.csi:md5,5bf5fc178e4faf2462427502c3666004"
+                "test2.recal.regions.bed.gz.csi:md5,2377c1d5f323117f03d96b4e83cd25cd"
             ],
             "No BAM files",
             [
@@ -242,11 +243,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:24:08.645314579",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T21:32:22.98324443"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test,spark --input tests/csv/3.0/fastq_tumor_only.csv --use_gatk_spark baserecalibrator,markduplicates --skip_tools fastqc,markduplicates_report,mosdepth,multiqc,samtools": {
         "content": [
@@ -275,10 +276,10 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "INDEX_MARKDUPLICATES": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/start_from_markduplicates.nf.test.snap b/tests/start_from_markduplicates.nf.test.snap
index dc2ee68e11..6147c20e10 100644
--- a/tests/start_from_markduplicates.nf.test.snap
+++ b/tests/start_from_markduplicates.nf.test.snap
@@ -20,13 +20,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -158,19 +159,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,d75ab155c53a8469919bee1c9808eb4b",
+                "samtools-stats-dp.txt:md5,8e1141a013873b3e9dfc7718fb8c7b23",
                 "samtools_alignment_plot.txt:md5,7a727168e9bbb3f4c39894933438df98",
                 "samtools_insert_size.txt:md5,c00ec0f338c40f2f1bc129f46ffb9084",
                 "test.md.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.md.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.md.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.md.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.md.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "test.md.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.recal.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.recal.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -180,11 +181,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:26:01.849929162",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:45:52.52962218"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step markduplicates --tools null": {
         "content": [
@@ -207,13 +208,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -345,19 +347,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,d75ab155c53a8469919bee1c9808eb4b",
+                "samtools-stats-dp.txt:md5,8e1141a013873b3e9dfc7718fb8c7b23",
                 "samtools_alignment_plot.txt:md5,7a727168e9bbb3f4c39894933438df98",
                 "samtools_insert_size.txt:md5,c00ec0f338c40f2f1bc129f46ffb9084",
                 "test.md.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.md.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.md.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.md.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.md.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "test.md.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,8e875e20e3fb9cf288d68c1d223f6fd5",
                 "test.recal.mosdepth.region.dist.txt:md5,75e1ce7e55af51f4985fa91654a5ea2d",
                 "test.recal.mosdepth.summary.txt:md5,b23cf96942b2ada3f41172a9349a1175",
                 "test.recal.regions.bed.gz:md5,74cd0c779c7b3228adcf3b177333886a",
-                "test.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917"
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c"
             ],
             "No BAM files",
             [
@@ -367,11 +369,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:40:21.440602423",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:48:22.238914133"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step markduplicates --skip_tools markduplicates --tools null": {
         "content": [
@@ -390,13 +392,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -503,19 +506,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,fcecb7f7ed584a1395065568e0ec1259",
                 "mosdepth_perchrom.txt:md5,4153678ed78ce01defe11f5b9fb8deb4",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
-                "samtools-stats-dp.txt:md5,dae2fa55a81049479a787e0fe5a64289",
+                "samtools-stats-dp.txt:md5,9dc8fae2db9d6106f819024bc3d8bad5",
                 "samtools_alignment_plot.txt:md5,b693942009526869bd21cbc42d54f53c",
                 "samtools_insert_size.txt:md5,7e95fe3ffb2346827b9e63da4de60842",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.sorted.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.sorted.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.sorted.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.sorted.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.sorted.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.sorted.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -524,11 +527,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:35:07.795402388",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:47:02.418026538"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_bam.csv --step markduplicates --skip_tools markduplicates --tools null": {
         "content": [
@@ -547,13 +550,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -660,19 +664,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,fcecb7f7ed584a1395065568e0ec1259",
                 "mosdepth_perchrom.txt:md5,4153678ed78ce01defe11f5b9fb8deb4",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
-                "samtools-stats-dp.txt:md5,dae2fa55a81049479a787e0fe5a64289",
+                "samtools-stats-dp.txt:md5,9dc8fae2db9d6106f819024bc3d8bad5",
                 "samtools_alignment_plot.txt:md5,b693942009526869bd21cbc42d54f53c",
                 "samtools_insert_size.txt:md5,7e95fe3ffb2346827b9e63da4de60842",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.sorted.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.sorted.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.sorted.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.sorted.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.sorted.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.sorted.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -681,10 +685,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:19:53.572042834",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:44:33.781719225"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/start_from_preparerecalibration.nf.test.snap b/tests/start_from_preparerecalibration.nf.test.snap
index cb9de0448b..e6c65e20ee 100644
--- a/tests/start_from_preparerecalibration.nf.test.snap
+++ b/tests/start_from_preparerecalibration.nf.test.snap
@@ -16,13 +16,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -124,14 +125,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
-                "samtools-stats-dp.txt:md5,c8deaa643c44fd800cbf14ac35fb7719",
+                "samtools-stats-dp.txt:md5,a30d6f7def3b7145015804ec4a4c3ee4",
                 "samtools_alignment_plot.txt:md5,8908b56b3040f2c53b15591adf93a266",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -140,11 +141,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:34:22.669878207",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:49:34.278947907"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step prepare_recalibration --skip_tools baserecalibrator --tools strelka": {
         "content": [
@@ -431,13 +432,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -539,14 +541,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,7d0b4b866fa577272c48a1f3ad72e75d",
-                "samtools-stats-dp.txt:md5,c8deaa643c44fd800cbf14ac35fb7719",
+                "samtools-stats-dp.txt:md5,a30d6f7def3b7145015804ec4a4c3ee4",
                 "samtools_alignment_plot.txt:md5,8908b56b3040f2c53b15591adf93a266",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -555,10 +557,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:27:50.357982281",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:52:41.43434759"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/start_from_recalibration.nf.test.snap b/tests/start_from_recalibration.nf.test.snap
index b5143c63bc..3c4d4773b6 100644
--- a/tests/start_from_recalibration.nf.test.snap
+++ b/tests/start_from_recalibration.nf.test.snap
@@ -13,13 +13,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -104,14 +105,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,c8deaa643c44fd800cbf14ac35fb7719",
+                "samtools-stats-dp.txt:md5,a30d6f7def3b7145015804ec4a4c3ee4",
                 "samtools_alignment_plot.txt:md5,8908b56b3040f2c53b15591adf93a266",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -120,11 +121,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:28:54.131141033",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:56:40.285535719"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step recalibrate --skip_tools baserecalibrator --tools strelka": {
         "content": [
@@ -408,13 +409,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -499,14 +501,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,c8deaa643c44fd800cbf14ac35fb7719",
+                "samtools-stats-dp.txt:md5,a30d6f7def3b7145015804ec4a4c3ee4",
                 "samtools_alignment_plot.txt:md5,8908b56b3040f2c53b15591adf93a266",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259"
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
             ],
             "No BAM files",
             [
@@ -515,10 +517,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:27:44.513392658",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:53:40.326206561"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 2e35492574..6c664c0a80 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -33,13 +33,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -338,7 +339,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,13dab249f7bef935dfef234299550db8",
+                "samtools-stats-dp.txt:md5,8b8f48d0b1d134cfe3636e8577265751",
                 "samtools_alignment_plot.txt:md5,44149e0c5cc4bfa58242824b300219a2",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
                 "test.strelka.variants.bcftools_stats.txt:md5,6efa6598982c21c544d1833dca632b62",
@@ -348,22 +349,22 @@
                 "test.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.md.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.md.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.md.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test.recal.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.recal.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.recal.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.recal.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.recal.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.recal.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test2.md.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.md.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.md.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.md.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.md.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.md.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test2.recal.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.recal.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.recal.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.recal.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.recal.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.recal.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test.strelka.variants.FILTER.summary:md5,dd87f507da7de20d5318841af312493b",
                 "test.strelka.variants.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "test2_vs_test.strelka.somatic_indels.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -389,10 +390,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-05-19T18:39:56.416042618",
+        "timestamp": "2026-07-27T10:29:54.04260346",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/umi_fastp.nf.test.snap b/tests/umi_fastp.nf.test.snap
index ce8a858825..e15a93581a 100644
--- a/tests/umi_fastp.nf.test.snap
+++ b/tests/umi_fastp.nf.test.snap
@@ -36,13 +36,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -324,7 +325,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,a6f11f838a72299e4913a54dd327f2e6",
+                "samtools-stats-dp.txt:md5,ab87568a8666df372d377d2dce411da3",
                 "samtools_alignment_plot.txt:md5,111d8b457e8c34dc919a527f12f27d4a",
                 "samtools_insert_size.txt:md5,5dbef29f8a260b9aa05de422676ab81a",
                 "test-test_L1.fastp.json:md5,5146ada16c3aae0e55e3e993b9d587af",
@@ -332,12 +333,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,94b080042f51f484fa178339cc9324bc",
                 "test.md.mosdepth.summary.txt:md5,7958c5422bb4b5181afe9782e38a735a",
                 "test.md.regions.bed.gz:md5,fe11b3aedde07e4e17114b5b37cdb3b8",
-                "test.md.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917",
+                "test.md.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test.recal.mosdepth.global.dist.txt:md5,40bf266b2080717f92b405cb42fab4a7",
                 "test.recal.mosdepth.region.dist.txt:md5,94b080042f51f484fa178339cc9324bc",
                 "test.recal.mosdepth.summary.txt:md5,7958c5422bb4b5181afe9782e38a735a",
                 "test.recal.regions.bed.gz:md5,fe11b3aedde07e4e17114b5b37cdb3b8",
-                "test.recal.regions.bed.gz.csi:md5,0dc011f3344841dc14aa488da905e917"
+                "test.recal.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0"
             ],
             "No BAM files",
             [
@@ -349,10 +350,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_umi_cleanname_1.fastq.gz): Cannot extract flowcell ID from @922332"
             ]
         ],
+        "timestamp": "2026-07-27T10:26:43.611284662",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T21:51:24.395033366"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/umi_fgbio.nf.test.snap b/tests/umi_fgbio.nf.test.snap
index da94f581b4..62a63cbb95 100644
--- a/tests/umi_fgbio.nf.test.snap
+++ b/tests/umi_fgbio.nf.test.snap
@@ -24,7 +24,8 @@
             29,
             {
                 "BAM2FASTQ": {
-                    "samtools": 1.21
+                    "bgzip": "1.24",
+                    "samtools": "1.24"
                 },
                 "BWAMEM1_INDEX": {
                     "bwa": "0.7.19-r1273"
@@ -40,10 +41,10 @@
                     "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "COLLATE_FASTQ_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -71,28 +72,29 @@
                     "fgbio": "3.1.2"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MERGE_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -300,19 +302,19 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,49a0910af4cea91b46a1df076c08c5b5",
+                "samtools-stats-dp.txt:md5,7575ba501ebbd93ba39f171443c0791b",
                 "samtools_alignment_plot.txt:md5,60b8f1d48124a8f28b06dc0d15ca4d59",
                 "samtools_insert_size.txt:md5,ef2a81138667814cda3e5c8c2628c549",
                 "test.md.mosdepth.global.dist.txt:md5,bb51e76c9cf2f3929cb5ce560f559dd6",
                 "test.md.mosdepth.region.dist.txt:md5,11dc4683f9038de1938b4874e02974d6",
                 "test.md.mosdepth.summary.txt:md5,d6cda5af0f2bf21973e256cf198aa47a",
                 "test.md.regions.bed.gz:md5,b420210d41279bbc93f211491f7d4f02",
-                "test.md.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.md.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.recal.mosdepth.global.dist.txt:md5,bb51e76c9cf2f3929cb5ce560f559dd6",
                 "test.recal.mosdepth.region.dist.txt:md5,11dc4683f9038de1938b4874e02974d6",
                 "test.recal.mosdepth.summary.txt:md5,d6cda5af0f2bf21973e256cf198aa47a",
                 "test.recal.regions.bed.gz:md5,b420210d41279bbc93f211491f7d4f02",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test_umi-grouped_histogram.txt:md5,85292e9acb83edf17110dce17be27f44",
                 "test_umi-grouped_read-metrics.txt:md5,cb2aecfe82357aa06e091d69b4606b9e"
             ],
@@ -328,11 +330,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_umi_cleanname_1.fastq.gz): Cannot extract flowcell ID from @922332"
             ]
         ],
+        "timestamp": "2026-07-27T10:25:14.330200197",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T21:53:07.910593016"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_umi.csv --umi_read_structure '+T 7M1S+T' --tools null --aligner sentieon-bwamem": {
         "content": [
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index 29202ec5b1..3b94ff9558 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -26,13 +26,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -233,7 +234,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,175b0442368f03294ef9ce9e73172c7b",
+                "samtools-stats-dp.txt:md5,e65c4559a631a2f575dfa4549b47c324",
                 "samtools_alignment_plot.txt:md5,6555bbf8c1ef12058e9a050545a9fa41",
                 "samtools_insert_size.txt:md5,164be68813f2cb96462c152743b95cae",
                 "test.strelka.variants.bcftools_stats.txt:md5,f4f3355bc1f101411c2fed6cf77d478e",
@@ -241,12 +242,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,54273a37adf55f8cee822aaf92a4c321",
                 "test.md.mosdepth.summary.txt:md5,bb12489d3d44c4e44eb5dcc8fdef636c",
                 "test.md.regions.bed.gz:md5,24cd07f8ce342a277f2954d6e731015e",
-                "test.md.regions.bed.gz.csi:md5,db6f77bcdbb7ada7e0c93e5b987b8265",
+                "test.md.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test.recal.mosdepth.global.dist.txt:md5,0c795eadf5e5a8ea8469ba8c0ece9bdd",
                 "test.recal.mosdepth.region.dist.txt:md5,54273a37adf55f8cee822aaf92a4c321",
                 "test.recal.mosdepth.summary.txt:md5,bb12489d3d44c4e44eb5dcc8fdef636c",
                 "test.recal.regions.bed.gz:md5,24cd07f8ce342a277f2954d6e731015e",
-                "test.recal.regions.bed.gz.csi:md5,db6f77bcdbb7ada7e0c93e5b987b8265",
+                "test.recal.regions.bed.gz.csi:md5,b21a943497a42870c5035d81a87524b0",
                 "test.strelka.variants.FILTER.summary:md5,f58effcb4006be9b10081e4417197aa6",
                 "test.strelka.variants.TsTv.count:md5,fccdacf16ebe20d74f36bd7b38f11ca4"
             ],
@@ -261,11 +262,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:36:55.243190299",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-09T09:52:02.012960987"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/bam_umi_header.csv --umi_in_read_header --tools markduplicates --step mapping": {
         "content": [
@@ -285,10 +286,10 @@
                     "cat": "9.5"
                 },
                 "COLLATE_FASTQ_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "COLLATE_FASTQ_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -313,28 +314,29 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MERGE_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_MAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_MAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_VIEW_UNMAP_UNMAP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -605,7 +607,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,afe461017b5f2189651f29c79f6fee44",
+                "samtools-stats-dp.txt:md5,99bedef59d485c544a016e3e372591d5",
                 "samtools_alignment_plot.txt:md5,9d6e0d529e64404f2c24e74285e242ee",
                 "samtools_insert_size.txt:md5,91c40430c1296fd8ce04788f2f528754",
                 "test.strelka.variants.bcftools_stats.txt:md5,a1500be145d3bd8a1e643c8772c4406d",
@@ -613,12 +615,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,1dd626f6fef0d1ac29fd7ffb8b1bbd95",
                 "test.md.mosdepth.summary.txt:md5,25af78b074abf710ddd9c90cba1928bc",
                 "test.md.regions.bed.gz:md5,813d852c5a237022fd4b7446d12f7772",
-                "test.md.regions.bed.gz.csi:md5,10b7f8e9ac2ac27205f8bc827a186195",
+                "test.md.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.recal.mosdepth.global.dist.txt:md5,7b8a7773d5d854ae1b681b971d11bb34",
                 "test.recal.mosdepth.region.dist.txt:md5,1dd626f6fef0d1ac29fd7ffb8b1bbd95",
                 "test.recal.mosdepth.summary.txt:md5,25af78b074abf710ddd9c90cba1928bc",
                 "test.recal.regions.bed.gz:md5,813d852c5a237022fd4b7446d12f7772",
-                "test.recal.regions.bed.gz.csi:md5,10b7f8e9ac2ac27205f8bc827a186195",
+                "test.recal.regions.bed.gz.csi:md5,551e8ad190dfafe3a1491661075a277c",
                 "test.strelka.variants.FILTER.summary:md5,869e416e19c9d8c7c6924758e66ea426",
                 "test.strelka.variants.TsTv.count:md5,2a049bc323b4bc559da89d136a567e28"
             ],
@@ -633,10 +635,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:42:25.348630085",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T22:06:00.327735562"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index c24a31fb1f..6402ccd10b 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -38,7 +38,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -66,10 +66,11 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MSISENSORPRO_MSISOMATIC": {
                     "msisensor-pro": "1.3.0"
@@ -78,7 +79,7 @@
                     "msisensor-pro": "1.3.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -526,7 +527,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,13dab249f7bef935dfef234299550db8",
+                "samtools-stats-dp.txt:md5,8b8f48d0b1d134cfe3636e8577265751",
                 "samtools_alignment_plot.txt:md5,44149e0c5cc4bfa58242824b300219a2",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
                 "test.bcftools.bcftools_stats.txt:md5,9213504c6b04c6c3cdf308ad3e8b4c3a",
@@ -542,22 +543,22 @@
                 "test.md.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.md.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.md.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.md.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.md.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test.recal.mosdepth.global.dist.txt:md5,76fa71922a3f748e507c2364c531dfcb",
                 "test.recal.mosdepth.region.dist.txt:md5,abc5df85e302b79985627888870882da",
                 "test.recal.mosdepth.summary.txt:md5,d536456436eb275159b8c6af83213d80",
                 "test.recal.regions.bed.gz:md5,b25a2798061021c0b2f4e1d18219bbbd",
-                "test.recal.regions.bed.gz.csi:md5,b1c2a861f64e20a94108a6de3b76c582",
+                "test.recal.regions.bed.gz.csi:md5,f58637ea3ed05bef11f054941c96e252",
                 "test2.md.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.md.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.md.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.md.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.md.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.md.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test2.recal.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.recal.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.recal.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.recal.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.recal.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.recal.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test.bcftools.FILTER.summary:md5,2c7c195b18f335698bae9619280492e8",
                 "test.bcftools.TsTv.count:md5,9748f609e7b83855e75804191c935ab3",
                 "test.deepvariant.FILTER.summary:md5,45b02f546c5f8a1365343130497190fa",
@@ -640,11 +641,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:30:48.429722692",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T16:20:15.694178204"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_single.csv --tools cnvkit,deepvariant,freebayes,mpileup,strelka,tiddit": {
         "content": [
@@ -685,7 +686,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -713,13 +714,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -1075,7 +1077,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ae7954084b2cd708c5e7369606bf4208",
+                "samtools-stats-dp.txt:md5,92dc10e61df31bf4286b16582bed488f",
                 "samtools_alignment_plot.txt:md5,438e719bf574a46726dbd2e0f1442e42",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
                 "test.bcftools.bcftools_stats.txt:md5,9213504c6b04c6c3cdf308ad3e8b4c3a",
@@ -1087,12 +1089,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.md.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.md.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.md.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.recal.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.recal.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.recal.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.recal.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.recal.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.recal.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.bcftools.FILTER.summary:md5,2c7c195b18f335698bae9619280492e8",
                 "test.bcftools.TsTv.count:md5,9748f609e7b83855e75804191c935ab3",
                 "test.deepvariant.FILTER.summary:md5,45b02f546c5f8a1365343130497190fa",
@@ -1140,11 +1142,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:32:11.483735223",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T16:23:41.706393706"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_tumor_only.csv --tools cnvkit,freebayes,mpileup,mutect2,tiddit": {
         "content": [
@@ -1179,7 +1181,7 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -1210,19 +1212,20 @@
                     "gatk4": "4.6.2.0"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "LEARNREADORIENTATIONMODEL": {
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1552,7 +1555,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,157a95de8aa4dd8947bb965f0c9ae3c7",
+                "samtools-stats-dp.txt:md5,c8a75397adcc418d4483c57a021550d4",
                 "samtools_alignment_plot.txt:md5,46e8824724863d243a01cc239a5eff15",
                 "samtools_insert_size.txt:md5,17c44517c58803f9c078f6d9b55aed00",
                 "test2.bcftools.bcftools_stats.txt:md5,fa4bd52259a3400e5d21c43b570e40a5",
@@ -1563,12 +1566,12 @@
                 "test2.md.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.md.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.md.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.md.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.md.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test2.recal.mosdepth.global.dist.txt:md5,2020cf6dfc7ddca020c921dd9f0549b7",
                 "test2.recal.mosdepth.region.dist.txt:md5,38ff8b38c33b9231f047fea8ea830aae",
                 "test2.recal.mosdepth.summary.txt:md5,8b991358768cade225470a07cd34f573",
                 "test2.recal.regions.bed.gz:md5,08e767f91a0a8d82733f0040e804a85f",
-                "test2.recal.regions.bed.gz.csi:md5,d5f1c9389ecf52ba839e834780a94549",
+                "test2.recal.regions.bed.gz.csi:md5,7357b11c67d045f7383a19845688e072",
                 "test2.bcftools.FILTER.summary:md5,2a717afe607d6b59558f860518738542",
                 "test2.bcftools.TsTv.count:md5,0c9a9764d605cba41d6c088340688939",
                 "test2.freebayes.filtered.FILTER.summary:md5,24e14f2e2651745a8d74dfe2844afa24",
@@ -1604,10 +1607,10 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:27:19.8576204",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T16:16:13.675589663"
+        }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_ascat.nf.test.snap b/tests/variant_calling_ascat.nf.test.snap
index 9e2892d7cf..d2078a5658 100644
--- a/tests/variant_calling_ascat.nf.test.snap
+++ b/tests/variant_calling_ascat.nf.test.snap
@@ -11,7 +11,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -106,7 +106,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -198,10 +198,11 @@
                     "ascat": "3.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -306,19 +307,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,c5095d8ac96d906979d5ead383b14d73",
                 "mosdepth_perchrom.txt:md5,a79d8d9cb20cf117f7510eb1e3674997",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,272184ca54d87094df9ab12335618e9a",
+                "samtools-stats-dp.txt:md5,a8448cbfa77d72de4e86a90bee0e5d17",
                 "samtools_alignment_plot.txt:md5,cd6531ba39758cf6ad96ba9d83df2230",
                 "samtools_insert_size.txt:md5,80b42e59a90818b77a39d87cd6b590e9",
                 "NA12878.recal.mosdepth.global.dist.txt:md5,a5ccac0290f8331d0aa7d68f0c9b0934",
                 "NA12878.recal.mosdepth.region.dist.txt:md5,81a999e01ee353b6a3dd5db321053ffc",
                 "NA12878.recal.mosdepth.summary.txt:md5,f78fade2ac186184fc44eb1d7ec443d0",
                 "NA12878.recal.regions.bed.gz:md5,2870532cc9d041c21999cb0a14d453b5",
-                "NA12878.recal.regions.bed.gz.csi:md5,58b5cd4cb9ab131a0f0a601eb72bcd04",
+                "NA12878.recal.regions.bed.gz.csi:md5,2cc4e07c8ef77b90542d238763002be9",
                 "NA12878_1X.recal.mosdepth.global.dist.txt:md5,8c70c08460d008b30c231a2d00fc3fb9",
                 "NA12878_1X.recal.mosdepth.region.dist.txt:md5,ddfe117ae7b0474c1e27857a282da9fb",
                 "NA12878_1X.recal.mosdepth.summary.txt:md5,8056360df7642e84fcc4355454cf617a",
                 "NA12878_1X.recal.regions.bed.gz:md5,0e6ff7afa2678e2dc667ec650bb18f75",
-                "NA12878_1X.recal.regions.bed.gz.csi:md5,5d07e60555f3087f5805dd20ed3bea8b",
+                "NA12878_1X.recal.regions.bed.gz.csi:md5,edac5d938e218575a6fd764accb7fbf2",
                 "NA12878_1X_vs_NA12878.cnvs.txt:md5,68b329da9893e34099c7d8ad5cb9c940",
                 "NA12878_1X_vs_NA12878.purityploidy.txt:md5,f1484c2b120834d3db8774ad02a038b9",
                 "NA12878_1X_vs_NA12878.segments.txt:md5,68b329da9893e34099c7d8ad5cb9c940",
@@ -334,10 +335,10 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2025-09-30T22:09:59.120270404",
+        "timestamp": "2026-07-27T10:27:42.266137545",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools ascat --input ascat_somatic.csv": {
@@ -349,10 +350,11 @@
                     "ascat": "3.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -455,19 +457,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,c5095d8ac96d906979d5ead383b14d73",
                 "mosdepth_perchrom.txt:md5,a79d8d9cb20cf117f7510eb1e3674997",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,272184ca54d87094df9ab12335618e9a",
+                "samtools-stats-dp.txt:md5,a8448cbfa77d72de4e86a90bee0e5d17",
                 "samtools_alignment_plot.txt:md5,cd6531ba39758cf6ad96ba9d83df2230",
                 "samtools_insert_size.txt:md5,80b42e59a90818b77a39d87cd6b590e9",
                 "NA12878.recal.mosdepth.global.dist.txt:md5,a5ccac0290f8331d0aa7d68f0c9b0934",
                 "NA12878.recal.mosdepth.region.dist.txt:md5,81a999e01ee353b6a3dd5db321053ffc",
                 "NA12878.recal.mosdepth.summary.txt:md5,f78fade2ac186184fc44eb1d7ec443d0",
                 "NA12878.recal.regions.bed.gz:md5,2870532cc9d041c21999cb0a14d453b5",
-                "NA12878.recal.regions.bed.gz.csi:md5,58b5cd4cb9ab131a0f0a601eb72bcd04",
+                "NA12878.recal.regions.bed.gz.csi:md5,2cc4e07c8ef77b90542d238763002be9",
                 "NA12878_1X.recal.mosdepth.global.dist.txt:md5,8c70c08460d008b30c231a2d00fc3fb9",
                 "NA12878_1X.recal.mosdepth.region.dist.txt:md5,ddfe117ae7b0474c1e27857a282da9fb",
                 "NA12878_1X.recal.mosdepth.summary.txt:md5,8056360df7642e84fcc4355454cf617a",
                 "NA12878_1X.recal.regions.bed.gz:md5,0e6ff7afa2678e2dc667ec650bb18f75",
-                "NA12878_1X.recal.regions.bed.gz.csi:md5,5d07e60555f3087f5805dd20ed3bea8b",
+                "NA12878_1X.recal.regions.bed.gz.csi:md5,edac5d938e218575a6fd764accb7fbf2",
                 "NA12878_1X_vs_NA12878.cnvs.txt:md5,68b329da9893e34099c7d8ad5cb9c940",
                 "NA12878_1X_vs_NA12878.purityploidy.txt:md5,f1484c2b120834d3db8774ad02a038b9",
                 "NA12878_1X_vs_NA12878.segments.txt:md5,68b329da9893e34099c7d8ad5cb9c940",
@@ -485,10 +487,10 @@
                 "WARN: No LogRCorrection performed in ASCAT. For LogRCorrection to run, please provide either loci gc files or both loci gc files and loci rt files."
             ]
         ],
-        "timestamp": "2025-12-15T22:00:03.974713999",
+        "timestamp": "2026-07-27T10:28:48.645382058",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_cnvkit.nf.test.snap b/tests/variant_calling_cnvkit.nf.test.snap
index e37886a5b3..c52d3d2793 100644
--- a/tests/variant_calling_cnvkit.nf.test.snap
+++ b/tests/variant_calling_cnvkit.nf.test.snap
@@ -22,16 +22,17 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -152,19 +153,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f7474a0afbf5565c5675916606f2d9bd",
                 "reference.cnn:md5,891454915f82d0eeda0be13d71e0d5d7",
@@ -198,11 +199,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:30:55.546225902",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:09:45.402105"
+        }
     },
     "-profile test --tools cnvkit --input recalibrated.csv --only_paired_variant_calling": {
         "content": [
@@ -227,16 +228,17 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -387,29 +389,29 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,ad0637d55d7025330f2f6cb7f9680e64",
                 "mosdepth_perchrom.txt:md5,73ef9a077df1887f9021a581fbf207bc",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
+                "samtools-stats-dp.txt:md5,a6501ad36c9d1e9b97878e69f072d63c",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f7474a0afbf5565c5675916606f2d9bd",
                 "reference.cnn:md5,891454915f82d0eeda0be13d71e0d5d7",
@@ -454,11 +456,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:28:58.93483756",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:18:52.335661"
+        }
     },
     "-profile test --tools cnvkit --input recalibrated_germline.csv": {
         "content": [
@@ -483,16 +485,17 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -588,14 +591,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f7474a0afbf5565c5675916606f2d9bd",
                 "reference.cnn:md5,891454915f82d0eeda0be13d71e0d5d7",
@@ -615,11 +618,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:26:30.003168858",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:05:28.442046"
+        }
     },
     "-profile test --tools cnvkit --input recalibrated_tumoronly.csv": {
         "content": [
@@ -644,16 +647,17 @@
                     "cnvkit": "0.9.11"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -748,14 +752,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "cnvkit.reference.antitarget-tmp.bed:md5,3d4d20f9f23b39970865d29ef239d20b",
                 "cnvkit.reference.target-tmp.bed:md5,657b25dbda8516624efa8cb2cf3716ca",
                 "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,067115082c4af4b64d58c0dc3a3642e4",
@@ -774,24 +778,25 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:31:08.508007983",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:13:42.841328"
+        }
     },
     "-profile test --tools cnvkit --input recalibrated_tumoronly.csv --no_intervals": {
         "content": [
             5,
             {
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -869,25 +874,25 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd"
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497"
             ],
             "No BAM files",
             "No CRAM files",
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:21:02.862389764",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:24:27.668069"
+        }
     },
     "-profile test --tools cnvkit --input recalibrated_somatic.csv --no_intervals": {
         "content": [
@@ -906,13 +911,14 @@
                     "cnvkit": "0.9.10"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1034,19 +1040,19 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.bed:md5,9c6cc178da8c2c27364be9f25c9df96d",
                 "genome.target.bed:md5,d6bb0e93de375af227a800a032f08f03",
@@ -1082,10 +1088,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:27:01.74112415",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-07-20T10:22:29.609745"
+        }
     }
 }
diff --git a/tests/variant_calling_controlfreec.nf.test.snap b/tests/variant_calling_controlfreec.nf.test.snap
index 2e78020160..e765355a00 100644
--- a/tests/variant_calling_controlfreec.nf.test.snap
+++ b/tests/variant_calling_controlfreec.nf.test.snap
@@ -19,13 +19,14 @@
                     "controlfreec": "11.6b"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MPILEUP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -121,13 +122,13 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,88b94dd2dcc423983da65125ece7651e",
                 "mosdepth_perchrom.txt:md5,a0266cd06dd6f7f5463f09e3cb8af71d",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.bed:md5,5249f46e614b60867bdd6b9b83327979",
                 "sample2.circos.txt:md5,2efab24d023931cec8b158c56d1f1765",
                 "sample2.p.value.txt:md5,38c8c9ad33a4fca3804a34d5c436cd1e",
@@ -143,10 +144,10 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-05-28T15:29:16.864765246",
+        "timestamp": "2026-07-27T10:37:16.056899008",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools controlfreec somatic": {
@@ -172,13 +173,14 @@
                     "controlfreec": "11.6b"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_MPILEUP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -289,23 +291,23 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4_vs_sample3.bed:md5,47f60179409e9389e59b2e2525e42210",
                 "sample4_vs_sample3.circos.txt:md5,68addb1d8bda08355842bef0ab15cd6e",
                 "sample4_vs_sample3.normal.mpileup.gz_control.cpn:md5,d50bf2c9a4d35f022364901c284e80ed",
@@ -322,10 +324,10 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-05-28T15:27:36.537227044",
+        "timestamp": "2026-07-27T10:29:23.11767132",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.2"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools controlfreec --no_intervals somatic -stub": {
@@ -351,10 +353,10 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_MPILEUP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -466,10 +468,10 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_MPILEUP": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
diff --git a/tests/variant_calling_deepvariant.nf.test.snap b/tests/variant_calling_deepvariant.nf.test.snap
index d713e12cbd..c04371b448 100644
--- a/tests/variant_calling_deepvariant.nf.test.snap
+++ b/tests/variant_calling_deepvariant.nf.test.snap
@@ -16,10 +16,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -164,7 +165,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.deepvariant.bcftools_stats.txt:md5,c784614aec9ed43f1d4a6149c8ae8bf3",
@@ -172,7 +173,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.deepvariant.FILTER.summary:md5,533b6a6a8eb84238b2d7ac6c229c1a7f",
                 "test.deepvariant.TsTv.count:md5,2851ce55f9813280515c2fdcacb86844"
             ],
@@ -184,10 +185,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2025-10-02T10:43:58.630637808",
+        "timestamp": "2026-07-27T10:44:10.97242938",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools deepvariant --input tests/csv/3.0/mapped_single_cram.csv -stub": {
@@ -210,7 +211,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -361,7 +362,7 @@
                     "mosdepth": "0.3.10"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -508,10 +509,11 @@
                     "deepvariant": "1.10.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -655,7 +657,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.deepvariant.bcftools_stats.txt:md5,c784614aec9ed43f1d4a6149c8ae8bf3",
@@ -663,7 +665,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.deepvariant.FILTER.summary:md5,533b6a6a8eb84238b2d7ac6c229c1a7f",
                 "test.deepvariant.TsTv.count:md5,2851ce55f9813280515c2fdcacb86844"
             ],
@@ -675,10 +677,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2025-10-02T10:45:45.83448312",
+        "timestamp": "2026-07-27T10:38:30.287152607",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index c8c585f4d9..1fdbe9b711 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -22,10 +22,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -183,7 +184,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,1036ea76acae803f591fd99838a8eded",
                 "mosdepth_perchrom.txt:md5,1f24f2f40467234c410a8bda544a8aae",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.freebayes.filtered.bcftools_stats.txt:md5,8d125501aef859ecb64b7363d93429fe",
@@ -191,9 +192,9 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample2.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample2.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample2.freebayes.filtered.FILTER.summary:md5,0a8ab31642123f2fc365df90166f22d5",
                 "sample2.freebayes.filtered.TsTv.count:md5,1756160098a27e7537c0fa8ea5cffc5f"
             ],
@@ -210,11 +211,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:28:53.984660271",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-04T12:14:50.842752"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools freebayes --no_intervals --wes --input fastq_pair.csv": {
         "content": [
@@ -250,13 +251,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -563,7 +565,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,7a0481b59cdd57cc0b8bc9b5641614c6",
+                "samtools-stats-dp.txt:md5,b4eaecbb6a9f2c463337ce06f8e2f47c",
                 "samtools_alignment_plot.txt:md5,8e6178a26fe2a4fc4f45fac3175ba6c6",
                 "samtools_insert_size.txt:md5,c4b5c78eeb30a8c926d1bd9506882516",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,5b0bc956256ff340d4f7409769fa9924",
@@ -571,19 +573,19 @@
                 "test.md.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.md.mosdepth.summary.txt:md5,0010c2396a3173c7cf4983abe2eb6a4c",
                 "test.md.per-base.bed.gz:md5,34dfe443c0a0767562dd65272e3310ef",
-                "test.md.per-base.bed.gz.csi:md5,b0ab630c3241fbd7581b7a38d944ff8b",
+                "test.md.per-base.bed.gz.csi:md5,635faec9b8aea3be719bc5cb9b81b1aa",
                 "test.recal.mosdepth.global.dist.txt:md5,5a0679057c530e5945c9c5a3a17312dc",
                 "test.recal.mosdepth.summary.txt:md5,0010c2396a3173c7cf4983abe2eb6a4c",
                 "test.recal.per-base.bed.gz:md5,34dfe443c0a0767562dd65272e3310ef",
-                "test.recal.per-base.bed.gz.csi:md5,b0ab630c3241fbd7581b7a38d944ff8b",
+                "test.recal.per-base.bed.gz.csi:md5,635faec9b8aea3be719bc5cb9b81b1aa",
                 "test2.md.mosdepth.global.dist.txt:md5,f25166c3a0051bb4d8c11a210278de6c",
                 "test2.md.mosdepth.summary.txt:md5,d5e4084de2ea2a0a7b60b2d71c804d4b",
                 "test2.md.per-base.bed.gz:md5,e1c6d60621d8f64aaf28fa1c1ddda921",
-                "test2.md.per-base.bed.gz.csi:md5,4205a09ede17cdbdaad45e3553f73105",
+                "test2.md.per-base.bed.gz.csi:md5,651986721bf7e1695fc044e06126df51",
                 "test2.recal.mosdepth.global.dist.txt:md5,f25166c3a0051bb4d8c11a210278de6c",
                 "test2.recal.mosdepth.summary.txt:md5,d5e4084de2ea2a0a7b60b2d71c804d4b",
                 "test2.recal.per-base.bed.gz:md5,e1c6d60621d8f64aaf28fa1c1ddda921",
-                "test2.recal.per-base.bed.gz.csi:md5,4205a09ede17cdbdaad45e3553f73105",
+                "test2.recal.per-base.bed.gz.csi:md5,651986721bf7e1695fc044e06126df51",
                 "test.freebayes.filtered.FILTER.summary:md5,87e753ba2ad969475fb55661852f75e0",
                 "test.freebayes.filtered.TsTv.count:md5,845f64e5bb4224af98f3a47294cd5483",
                 "test2_vs_test.freebayes.filtered.FILTER.summary:md5,1f06f857e3576c6a98c32d1e7f51f456",
@@ -621,11 +623,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:31:12.762942445",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T11:36:14.240620697"
+        }
     },
     "-profile test --tools freebayes --wes --nucleotides_per_second 20": {
         "content": [
@@ -667,19 +669,20 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_FREEBAYES": {
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -957,7 +960,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,300b95526a211b05f18aaffd037dacd3",
+                "samtools-stats-dp.txt:md5,2f8b6ccd23ecf736af3d32dbb33699b2",
                 "samtools_alignment_plot.txt:md5,5053f650b8612fe5e8527b0bca8ab905",
                 "samtools_insert_size.txt:md5,2b1da7c476290081135a2816f4231333",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,b0d8c9234a3b038c05fd14d656e43956",
@@ -965,16 +968,16 @@
                 "test.md.mosdepth.region.dist.txt:md5,d25723bdd3fec6b17d2462abfa097b9e",
                 "test.md.mosdepth.summary.txt:md5,87be70cd1237d7af9aa40d8cd8b3a817",
                 "test.md.per-base.bed.gz:md5,c53d26b767b6e75b3e502438a77f89b2",
-                "test.md.per-base.bed.gz.csi:md5,c3066b00781e14a9db5fc0bf0d47d777",
+                "test.md.per-base.bed.gz.csi:md5,df3802b73cb56d0301af323b18341e99",
                 "test.md.regions.bed.gz:md5,f96fa1cdae548eb7e54ce6a481d928b9",
-                "test.md.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test.md.regions.bed.gz.csi:md5,2cdd53425271b3ada1ee10958372331b",
                 "test.recal.mosdepth.global.dist.txt:md5,a3e6c8f6d4b5e909d0527be83a93fbae",
                 "test.recal.mosdepth.region.dist.txt:md5,d25723bdd3fec6b17d2462abfa097b9e",
                 "test.recal.mosdepth.summary.txt:md5,ca5424a709268a61200a2dc2865f1a14",
                 "test.recal.per-base.bed.gz:md5,8aaf9cb3dd5c9643e77aba91293fc39d",
-                "test.recal.per-base.bed.gz.csi:md5,d8038c7d544abd5d6335f2541de4e769",
+                "test.recal.per-base.bed.gz.csi:md5,4bbe5c3dc2ea4bdcb40a47aff885c7bb",
                 "test.recal.regions.bed.gz:md5,f96fa1cdae548eb7e54ce6a481d928b9",
-                "test.recal.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test.recal.regions.bed.gz.csi:md5,2cdd53425271b3ada1ee10958372331b",
                 "test.freebayes.filtered.FILTER.summary:md5,259470a9823d503d5639946162b2ed19",
                 "test.freebayes.filtered.TsTv.count:md5,162253eb6c406300678985b3ac7dc868"
             ],
@@ -996,11 +999,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:29:15.12436469",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T11:27:28.609439333"
+        }
     },
     "-profile test --tools freebayes --no_intervals": {
         "content": [
@@ -1036,13 +1039,14 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1318,7 +1322,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,9e836f09043529495de9e1c268ee8e82",
+                "samtools-stats-dp.txt:md5,35b5d272beeaf4a4b926fd3ace473928",
                 "samtools_alignment_plot.txt:md5,bcc2f176a4bc51b33a36bd4381f048a5",
                 "samtools_insert_size.txt:md5,73b933a27800b86a4012c2d525870796",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,60f55a391db494db3e95bd69b6b36fa3",
@@ -1326,12 +1330,12 @@
                 "test.md.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.md.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.md.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.md.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.md.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.recal.mosdepth.global.dist.txt:md5,ef7c375ae07aec5540f9892b9b556b73",
                 "test.recal.mosdepth.region.dist.txt:md5,212efff2213f6fc1c3204daf68bbb8c8",
                 "test.recal.mosdepth.summary.txt:md5,72114393647ff64503522760218b30f0",
                 "test.recal.regions.bed.gz:md5,985db429051ddcd5eae177da6fb55ad6",
-                "test.recal.regions.bed.gz.csi:md5,3fa0f8272fefafe3cd840376d34a94a2",
+                "test.recal.regions.bed.gz.csi:md5,1f17d398be6b9c100cb0218fa226a623",
                 "test.freebayes.filtered.FILTER.summary:md5,9ae0931339e231f90a4b5c330f7f6d55",
                 "test.freebayes.filtered.TsTv.count:md5,845f64e5bb4224af98f3a47294cd5483"
             ],
@@ -1353,11 +1357,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:32:49.236918224",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T11:30:11.341607202"
+        }
     },
     "-profile test --tools freebayes --no_intervals --input recalibrated_tumoronly.csv": {
         "content": [
@@ -1376,10 +1380,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1536,14 +1541,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,88b94dd2dcc423983da65125ece7651e",
                 "mosdepth_perchrom.txt:md5,a0266cd06dd6f7f5463f09e3cb8af71d",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.freebayes.filtered.bcftools_stats.txt:md5,6bc59a78f70ac18e8d69a779a7892673",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.freebayes.filtered.FILTER.summary:md5,fac42829b9b347413acbfc9c0a17fef0",
                 "sample2.freebayes.filtered.TsTv.count:md5,f864a8b1d6bab1d5f877b60dde36863f"
             ],
@@ -1560,11 +1565,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:27:39.737452077",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.10.0"
-        },
-        "timestamp": "2025-11-04T12:22:29.186457"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools freebayes --wes --nucleotides_per_second 20 --input fastq_pair.csv": {
         "content": [
@@ -1606,19 +1611,20 @@
                     "samtools": "1.21"
                 },
                 "INDEX_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_CRAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "MERGE_FREEBAYES": {
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1935,7 +1941,7 @@
                 "picard_MeanQualityByCycle_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_MeanQualityByCycle_histogram_1.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "picard_QualityScoreDistribution_histogram.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
-                "samtools-stats-dp.txt:md5,ff7906db0da2f9c9ba5ce85c34694124",
+                "samtools-stats-dp.txt:md5,51e64be3231a16d20f89f2e8bac9b93a",
                 "samtools_alignment_plot.txt:md5,89d0a6e7076223e9feadbecd794948d5",
                 "samtools_insert_size.txt:md5,ca54b785b1d63edd61cef58cd5558aa2",
                 "test.freebayes.filtered.bcftools_stats.txt:md5,49399853673a44189a06f171efb1c670",
@@ -1944,30 +1950,30 @@
                 "test.md.mosdepth.region.dist.txt:md5,835fdc6fa52cc33e6fb76c0c20a8a6c3",
                 "test.md.mosdepth.summary.txt:md5,dcc9ab2bf3248903e02d8da87e678977",
                 "test.md.per-base.bed.gz:md5,34dfe443c0a0767562dd65272e3310ef",
-                "test.md.per-base.bed.gz.csi:md5,b0ab630c3241fbd7581b7a38d944ff8b",
+                "test.md.per-base.bed.gz.csi:md5,635faec9b8aea3be719bc5cb9b81b1aa",
                 "test.md.regions.bed.gz:md5,99cc80b920ba574e7d9ef8f59f54f7c6",
-                "test.md.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test.md.regions.bed.gz.csi:md5,2cdd53425271b3ada1ee10958372331b",
                 "test.recal.mosdepth.global.dist.txt:md5,0b3162def977123809598639f7698121",
                 "test.recal.mosdepth.region.dist.txt:md5,835fdc6fa52cc33e6fb76c0c20a8a6c3",
                 "test.recal.mosdepth.summary.txt:md5,a8455eb2947de529abfa62b303986e0f",
                 "test.recal.per-base.bed.gz:md5,c075ccd2b847c7c04061a39717faeb30",
-                "test.recal.per-base.bed.gz.csi:md5,4816eeb9af254ca40177b08cf11b98d2",
+                "test.recal.per-base.bed.gz.csi:md5,93782f791ac4333f6cfc02d6f38d67bc",
                 "test.recal.regions.bed.gz:md5,99cc80b920ba574e7d9ef8f59f54f7c6",
-                "test.recal.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test.recal.regions.bed.gz.csi:md5,2cdd53425271b3ada1ee10958372331b",
                 "test2.md.mosdepth.global.dist.txt:md5,f25166c3a0051bb4d8c11a210278de6c",
                 "test2.md.mosdepth.region.dist.txt:md5,3211135329e4077bd9bf0ba488e14371",
                 "test2.md.mosdepth.summary.txt:md5,ce0eb6d33c6d0dc720fbc6d1811abef8",
                 "test2.md.per-base.bed.gz:md5,e1c6d60621d8f64aaf28fa1c1ddda921",
-                "test2.md.per-base.bed.gz.csi:md5,4205a09ede17cdbdaad45e3553f73105",
+                "test2.md.per-base.bed.gz.csi:md5,651986721bf7e1695fc044e06126df51",
                 "test2.md.regions.bed.gz:md5,0bb2549180165a99680ba3e453ea312f",
-                "test2.md.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test2.md.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "test2.recal.mosdepth.global.dist.txt:md5,a1ef7e662ce993da4668e804952014ce",
                 "test2.recal.mosdepth.region.dist.txt:md5,3211135329e4077bd9bf0ba488e14371",
                 "test2.recal.mosdepth.summary.txt:md5,70ad653c0c98baeeaf5085f1209a7bdb",
                 "test2.recal.per-base.bed.gz:md5,e992ef845ec91a3612297952a23ba579",
-                "test2.recal.per-base.bed.gz.csi:md5,8072f447199c60f24b01eede8b557333",
+                "test2.recal.per-base.bed.gz.csi:md5,0f5fca336438af0a76a853c44af95b26",
                 "test2.recal.regions.bed.gz:md5,0bb2549180165a99680ba3e453ea312f",
-                "test2.recal.regions.bed.gz.csi:md5,c6d1ac97ef4dfe43731c8368d8391cab",
+                "test2.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "test.freebayes.filtered.FILTER.summary:md5,449597c35ada505b4cb2530d5260e9d5",
                 "test.freebayes.filtered.TsTv.count:md5,162253eb6c406300678985b3ac7dc868",
                 "test2_vs_test.freebayes.filtered.FILTER.summary:md5,b3cdcab0fb3a666b40ef76c4193ff7f8",
@@ -1999,10 +2005,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-27T10:34:34.523815105",
         "meta": {
-            "nf-test": "0.9.3",
+            "nf-test": "0.9.5",
             "nextflow": "25.10.4"
-        },
-        "timestamp": "2026-06-12T11:33:20.327931788"
+        }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_haplotypecaller.nf.test.snap b/tests/variant_calling_haplotypecaller.nf.test.snap
index 7016f600b7..55281a9cb3 100644
--- a/tests/variant_calling_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_haplotypecaller.nf.test.snap
@@ -10,10 +10,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -158,14 +159,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,24098c80b7a75e41cd68ef1f2154b7b0",
                 "mosdepth_perchrom.txt:md5,93a8fd4ad5c5a4fcdf9e67a07183c7cb",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotypecaller.bcftools_stats.txt:md5,b9b8c3cb422534bdb7a45d884c121af8",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.summary.txt:md5,4f0d231060cbde4efdd673863bd2fb59",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "test.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "test.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "test.haplotypecaller.FILTER.summary:md5,87a84b5f8ac3d3cbeeef7d60afcdbfe7",
                 "test.haplotypecaller.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -176,11 +177,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:27:52.965412156",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T22:57:09.952875456"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input mapped_single_bam.csv --tools haplotypecaller --step variant_calling --wes --nucleotides_per_second 20 --skip_tools haplotypecaller_filter": {
         "content": [
@@ -199,10 +200,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -351,7 +353,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cff5ad3df703fd30cd655ef53caf75fb",
                 "mosdepth_perchrom.txt:md5,6e1f4a26793f1912cd83cee7259635c6",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotypecaller.bcftools_stats.txt:md5,b9b8c3cb422534bdb7a45d884c121af8",
@@ -359,9 +361,9 @@
                 "test.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "test.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "test.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "test.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "test.recal.regions.bed.gz:md5,0c8215fbea7b0bf7aba9d1781575f905",
-                "test.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "test.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "test.haplotypecaller.FILTER.summary:md5,87a84b5f8ac3d3cbeeef7d60afcdbfe7",
                 "test.haplotypecaller.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -372,11 +374,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:29:55.289787759",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T22:56:07.158030738"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input mapped_single_bam.csv --tools haplotypecaller --step variant_calling --wes --nucleotides_per_second 20 --no_intervals": {
         "content": [
@@ -395,10 +397,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -545,14 +548,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,24098c80b7a75e41cd68ef1f2154b7b0",
                 "mosdepth_perchrom.txt:md5,93a8fd4ad5c5a4fcdf9e67a07183c7cb",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotypecaller.filtered.bcftools_stats.txt:md5,5debbaa917411dda07c7972272a95f38",
                 "test.recal.mosdepth.global.dist.txt:md5,e82e90c7d508a135b5a8a7cd6933452e",
                 "test.recal.mosdepth.summary.txt:md5,4f0d231060cbde4efdd673863bd2fb59",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "test.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "test.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "test.haplotypecaller.filtered.FILTER.summary:md5,4e2ceea7f3ff998004691fd71192d9ee",
                 "test.haplotypecaller.filtered.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -564,11 +567,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:32:53.636129324",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T22:54:55.763188536"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input mapped_single_bam.csv --tools haplotypecaller --step variant_calling --wes --nucleotides_per_second 20": {
         "content": [
@@ -593,10 +596,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -747,7 +751,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cff5ad3df703fd30cd655ef53caf75fb",
                 "mosdepth_perchrom.txt:md5,6e1f4a26793f1912cd83cee7259635c6",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotypecaller.filtered.bcftools_stats.txt:md5,5debbaa917411dda07c7972272a95f38",
@@ -755,9 +759,9 @@
                 "test.recal.mosdepth.region.dist.txt:md5,3a2030e5e8af7bc12720c3a5592bf921",
                 "test.recal.mosdepth.summary.txt:md5,615c5c5019d88045a9ff5bbe6e63d270",
                 "test.recal.per-base.bed.gz:md5,da6db0fb375a3053a89db8c935eebbaa",
-                "test.recal.per-base.bed.gz.csi:md5,9e649ac749ff6c6073bef5ab63e8aaa4",
+                "test.recal.per-base.bed.gz.csi:md5,524a822fc4863b450fda9e0f99ccdf04",
                 "test.recal.regions.bed.gz:md5,0c8215fbea7b0bf7aba9d1781575f905",
-                "test.recal.regions.bed.gz.csi:md5,5c00a1d457c387d6e71848a6d897e309",
+                "test.recal.regions.bed.gz.csi:md5,8f139abca5ac58eaf522bd96a46cff1b",
                 "test.haplotypecaller.filtered.FILTER.summary:md5,4e2ceea7f3ff998004691fd71192d9ee",
                 "test.haplotypecaller.filtered.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -769,10 +773,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:32:48.438182421",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T22:53:34.886097542"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_lofreq.nf.test.snap b/tests/variant_calling_lofreq.nf.test.snap
index dae6d760ce..74a9f15e41 100644
--- a/tests/variant_calling_lofreq.nf.test.snap
+++ b/tests/variant_calling_lofreq.nf.test.snap
@@ -16,10 +16,11 @@
                     "lofreq": "2.1.5"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -161,7 +162,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,1036ea76acae803f591fd99838a8eded",
                 "mosdepth_perchrom.txt:md5,1f24f2f40467234c410a8bda544a8aae",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.lofreq.bcftools_stats.txt:md5,064a0729f34ff2b74a1ea619cc47ecb6",
@@ -169,9 +170,9 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample2.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample2.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample2.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample2.lofreq.FILTER.summary:md5,8dd8a0c91d5c4a260b462e04f615e502"
             ],
             "No BAM files",
@@ -184,10 +185,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:31:16.337399595",
+        "timestamp": "2026-07-27T10:22:20.09841282",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools lofreq --no_intervals tumoronly": {
@@ -204,10 +205,11 @@
                     "lofreq": "2.1.5"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -345,14 +347,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,88b94dd2dcc423983da65125ece7651e",
                 "mosdepth_perchrom.txt:md5,a0266cd06dd6f7f5463f09e3cb8af71d",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.lofreq.bcftools_stats.txt:md5,1aa21f81ba86f60f145934ab517fc44e",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.lofreq.FILTER.summary:md5,72beda1b57da053eb352204828605a40"
             ],
             "No BAM files",
@@ -365,10 +367,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:32:37.387358619",
+        "timestamp": "2026-07-27T10:30:44.706814777",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_manta.nf.test.snap b/tests/variant_calling_manta.nf.test.snap
index b24430a374..c9a50c3d9a 100644
--- a/tests/variant_calling_manta.nf.test.snap
+++ b/tests/variant_calling_manta.nf.test.snap
@@ -13,10 +13,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -133,7 +134,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
@@ -141,7 +142,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample1.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -152,10 +153,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:43:23.100191682",
+        "timestamp": "2026-07-27T10:45:08.642654705",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta --no_intervals tumoronly": {
@@ -169,10 +170,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -288,7 +290,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.manta.tumor_sv.bcftools_stats.txt:md5,6a4c5f596d407e12449c36fe3419488f",
@@ -296,7 +298,7 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample2.manta.tumor_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample2.manta.tumor_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -307,10 +309,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:48:58.932904711",
+        "timestamp": "2026-07-27T10:32:07.479458904",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta --no_intervals somatic": {
@@ -327,10 +329,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -469,7 +472,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
@@ -479,12 +482,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample3.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample4_vs_sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -501,10 +504,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:46:50.07261297",
+        "timestamp": "2026-07-27T10:33:53.269385428",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta tumoronly": {
@@ -521,10 +524,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -641,7 +645,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.manta.tumor_sv.bcftools_stats.txt:md5,6a4c5f596d407e12449c36fe3419488f",
@@ -649,7 +653,7 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample2.manta.tumor_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample2.manta.tumor_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -660,10 +664,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:47:53.602614087",
+        "timestamp": "2026-07-27T10:35:30.836798406",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta --only_paired_variant_calling": {
@@ -686,10 +690,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -854,7 +859,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,ad0637d55d7025330f2f6cb7f9680e64",
                 "mosdepth_perchrom.txt:md5,73ef9a077df1887f9021a581fbf207bc",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
+                "samtools-stats-dp.txt:md5,a6501ad36c9d1e9b97878e69f072d63c",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
                 "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
@@ -865,22 +870,22 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample1.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample1.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample2.manta.tumor_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -900,10 +905,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:42:13.91617972",
+        "timestamp": "2026-07-27T10:38:31.008536362",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta somatic": {
@@ -923,10 +928,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1066,7 +1072,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
@@ -1076,12 +1082,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample3.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample4_vs_sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -1098,10 +1104,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:45:35.30977089",
+        "timestamp": "2026-07-27T10:30:18.506893012",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools manta --no_intervals germline": {
@@ -1115,10 +1121,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -1234,7 +1241,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.manta.diploid_sv.bcftools_stats.txt:md5,8721040d8aa617f37498e9d89f48d503",
@@ -1242,7 +1249,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample1.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -1253,10 +1260,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:44:26.578484488",
+        "timestamp": "2026-07-27T10:39:22.908367447",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_mpileup.nf.test.snap b/tests/variant_calling_mpileup.nf.test.snap
index d4536963aa..cd66e0b5f8 100644
--- a/tests/variant_calling_mpileup.nf.test.snap
+++ b/tests/variant_calling_mpileup.nf.test.snap
@@ -13,10 +13,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -163,7 +164,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.bcftools.bcftools_stats.txt:md5,47ce340432efdbb38a86d8b7c3d0c2d4",
@@ -171,7 +172,7 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample2.bcftools.FILTER.summary:md5,8766995f3e4119ef30dfdaa9fb3752ce",
                 "sample2.bcftools.TsTv.count:md5,01df95fcb4df593f7e1b214d90ebdb59"
             ],
@@ -182,11 +183,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:33:46.74937531",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:12:38.089637491"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mpileup --input recalibrated_germline.csv --no_intervals": {
         "content": [
@@ -199,10 +200,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -348,7 +350,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.bcftools.bcftools_stats.txt:md5,81ab4ccc0da202a814b7f99ba5c49bb4",
@@ -356,7 +358,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.bcftools.FILTER.summary:md5,83a10512eb9d035f409a84db7e620c28",
                 "sample1.bcftools.TsTv.count:md5,bfa998e75cbcb3da66f823cf39ef1e48"
             ],
@@ -367,11 +369,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:28:38.96226408",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:11:16.876484642"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mpileup --input recalibrated_tumoronly.csv --no_intervals": {
         "content": [
@@ -384,10 +386,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -533,7 +536,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.bcftools.bcftools_stats.txt:md5,4897ad81224f076a9d7054ba865de378",
@@ -541,7 +544,7 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample2.bcftools.FILTER.summary:md5,f295c70f174e7705fc2bac607aedbfda",
                 "sample2.bcftools.TsTv.count:md5,e5d20f81fc97f7ee97fb6cb6bd851047"
             ],
@@ -552,11 +555,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:33:54.21084607",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:14:01.579156158"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mpileup --input recalibrated_germline.csv": {
         "content": [
@@ -572,10 +575,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -722,7 +726,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.bcftools.bcftools_stats.txt:md5,51bc17eed97a82f7a4a59d5f8181a1cc",
@@ -730,7 +734,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.bcftools.FILTER.summary:md5,9b62595b026decf12e9198d531e4307a",
                 "sample1.bcftools.TsTv.count:md5,6c937125d7bac4c491bea50f18cba43a"
             ],
@@ -741,10 +745,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:29:53.620097595",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:09:53.430122786"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_msisensor2.nf.test.snap b/tests/variant_calling_msisensor2.nf.test.snap
index 23997e71d4..db76dbf777 100644
--- a/tests/variant_calling_msisensor2.nf.test.snap
+++ b/tests/variant_calling_msisensor2.nf.test.snap
@@ -167,13 +167,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MSISENSOR2_MSI": {
                     "msisensor2": 0.1
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -266,10 +267,10 @@
                 "test.recal.mosdepth.global.dist.txt:md5,96d22fe7b6f5824cde06c98c96dec304",
                 "test.recal.mosdepth.region.dist.txt:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "test.recal.mosdepth.summary.txt:md5,81c058bd237404d95b3f838bae176303",
-                "test.recal.per-base.bed.gz:md5,bafb7489cc40a55cfe85f99ea6d36cc7",
-                "test.recal.per-base.bed.gz.csi:md5,e72e7c7886d42728d785bb97dffd2a07",
+                "test.recal.per-base.bed.gz:md5,557eec61090bc149d9a9fecbfd5774cb",
+                "test.recal.per-base.bed.gz.csi:md5,4a3a6c9d1af60bd3c2ddef8ce088ffa3",
                 "test.recal.regions.bed.gz:md5,d7e8ac59cdfd4cfb70712e699c6848f5",
-                "test.recal.regions.bed.gz.csi:md5,d9cfb4c9a4ffcf4b0122c378a547162a",
+                "test.recal.regions.bed.gz.csi:md5,e30cfcd01d332c367656668d87ecce62",
                 "test:md5,a3290f7539dbbf83777e8590156c0e28",
                 "test_dis:md5,85205504ea8652ebdee46ac07ee1a8f6"
             ],
@@ -278,10 +279,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:30:44.993560881",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.3"
-        },
-        "timestamp": "2026-02-09T09:57:39.631656166"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_msisensorpro.nf.test.snap b/tests/variant_calling_msisensorpro.nf.test.snap
index 82da407438..e5d36cb0d3 100644
--- a/tests/variant_calling_msisensorpro.nf.test.snap
+++ b/tests/variant_calling_msisensorpro.nf.test.snap
@@ -56,7 +56,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MSISENSORPRO_MSISOMATIC": {
                     "msisensor-pro": "1.3.0"
@@ -65,7 +66,7 @@
                     "msisensor-pro": "1.3.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -163,23 +164,23 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,6239b0f0a9736ccaf3baff3014dd585b",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4_vs_sample3:md5,efc7a09642d444d7475d976c7c8110f4",
                 "sample4_vs_sample3_dis:md5,780b282473e51808c5ae32b50b4a6406"
             ],
@@ -188,11 +189,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:23:15.906308902",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:18:49.269683325"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools msisensorpro somatic --build_only_index --input false": {
         "content": [
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index cb8540dbbb..68982cfc30 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -7,7 +7,7 @@
                     "bcftools": "1.23.1"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -26,7 +26,7 @@
                     "muse": "2.1.2"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -187,7 +187,7 @@
                     "bcftools": "1.23.1"
                 },
                 "CRAM_TO_BAM": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -196,7 +196,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUSE_CALL": {
                     "muse": "2.1.2"
@@ -206,7 +207,7 @@
                     "muse": "2.1.2"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -337,7 +338,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample4_vs_sample3.muse.bcftools_stats.txt:md5,78caff99ab648b7191fd74954713aef9",
@@ -345,16 +346,16 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4_vs_sample3.muse.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample4_vs_sample3.muse.TsTv.count:md5,8dcfdbcaac118df1d5ad407dd2af699f"
             ],
@@ -368,10 +369,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-19T18:51:58.229719817",
+        "timestamp": "2026-07-27T10:39:31.857457867",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index c905361d6e..0a33bc5519 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -13,13 +13,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -164,14 +165,14 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,88b94dd2dcc423983da65125ece7651e",
                 "mosdepth_perchrom.txt:md5,a0266cd06dd6f7f5463f09e3cb8af71d",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.mutect2.bcftools_stats.txt:md5,634af596519997cd802fbc8fe290342f",
                 "sample2.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.mutect2.FILTER.summary:md5,08f06620a8dcc70115bdde9137a91008",
                 "sample2.mutect2.TsTv.count:md5,0d59dcbdb127be60909111958ff7b5f5",
                 "sample2.mutect2.vcf.gz.stats:md5,76f749c53212d72e98801f6030fbf8a6"
@@ -186,11 +187,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:36:37.905823869",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T23:34:19.18363343"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mutect2 somatic": {
         "content": [
@@ -209,13 +210,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -374,7 +376,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,cb7468f51b8be1230fb3ac5b130be31f",
                 "mosdepth_perchrom.txt:md5,4ee05f71086179b42a01cd2fb450346f",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,c802eeeaa4ab6531bdb5d8c0b583f8b6",
@@ -382,16 +384,16 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,6ec49cd7d510c2eb3d9d90fdb79b783a",
                 "sample3.recal.mosdepth.summary.txt:md5,103098d0bf76ed82d2b87d5f242b099a",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample3.recal.regions.bed.gz:md5,314ce8d7273eff353072108aa77c327c",
-                "sample3.recal.regions.bed.gz.csi:md5,9cb0ad7039a3b703d16ca7d5b835c0ee",
+                "sample3.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.region.dist.txt:md5,39005ffaac22871ffaaf19656fe69c5b",
                 "sample4.recal.mosdepth.summary.txt:md5,68d4b98f17361fddf73052ead34fa370",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4.recal.regions.bed.gz:md5,b7561bc56a955f7db0f11e67e2ec0386",
-                "sample4.recal.regions.bed.gz.csi:md5,393c2749068304d8545b501b9d4658e4",
+                "sample4.recal.regions.bed.gz.csi:md5,2c9ca91a9535113038c59b6e408e0818",
                 "sample4_vs_sample3.mutect2.FILTER.summary:md5,cac64448be577632a614af62a23af34a",
                 "sample4_vs_sample3.mutect2.TsTv.count:md5,3739f24da2d2019cc4bc2821e30658eb",
                 "sample4_vs_sample3.mutect2.vcf.gz.stats:md5,bd657dd9abf6e2354224bb0d20ba181e"
@@ -406,11 +408,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:49:05.16697888",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T23:23:51.624986383"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mutect2 somatic --no_intervals": {
         "content": [
@@ -426,13 +428,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2_PAIRED": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -584,18 +587,18 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,35fe7c32ea54f1a69c647202873bb7d7",
                 "mosdepth_perchrom.txt:md5,14473da27940bc99c7b9ed36d82f7429",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample4_vs_sample3.mutect2.bcftools_stats.txt:md5,c802eeeaa4ab6531bdb5d8c0b583f8b6",
                 "sample3.recal.mosdepth.global.dist.txt:md5,69e29702ef01fd8f6c7a5468fc35a16a",
                 "sample3.recal.mosdepth.summary.txt:md5,d2775eb102acc5950f7f53883dcb503d",
                 "sample3.recal.per-base.bed.gz:md5,297f96648928d0ca5184223fb9941e7c",
-                "sample3.recal.per-base.bed.gz.csi:md5,c67dcd711b096eb42f43784d5eadbc0d",
+                "sample3.recal.per-base.bed.gz.csi:md5,68451158beb41ef7fcd07821fe3a52b4",
                 "sample4.recal.mosdepth.global.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample4.recal.mosdepth.summary.txt:md5,0a7300e56eda6fba7c7564f00aa000f0",
                 "sample4.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample4.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample4.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample4_vs_sample3.mutect2.FILTER.summary:md5,cac64448be577632a614af62a23af34a",
                 "sample4_vs_sample3.mutect2.TsTv.count:md5,3739f24da2d2019cc4bc2821e30658eb",
                 "sample4_vs_sample3.mutect2.vcf.gz.stats:md5,4300e84631ee258660f95e846511d021"
@@ -610,11 +613,11 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:42:39.618205653",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T23:27:38.999681674"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools mutect2 tumoronly": {
         "content": [
@@ -636,13 +639,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "MUTECT2": {
                     "gatk4": "4.6.2.0"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -791,7 +795,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,88b94dd2dcc423983da65125ece7651e",
                 "mosdepth_perchrom.txt:md5,a0266cd06dd6f7f5463f09e3cb8af71d",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.mutect2.bcftools_stats.txt:md5,634af596519997cd802fbc8fe290342f",
@@ -799,9 +803,9 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,f2dcd00a64947c49e8e4b93c2f4fbf27",
                 "sample2.recal.mosdepth.summary.txt:md5,b0b47739dcafeeb1a9e6218b8abca1e0",
                 "sample2.recal.per-base.bed.gz:md5,39a1bc436aa8546c26faedbe94cb676c",
-                "sample2.recal.per-base.bed.gz.csi:md5,cfb07b0ba46e8468b4342edb243536f3",
+                "sample2.recal.per-base.bed.gz.csi:md5,8964f1acc52b73fe72deffcd39e98d78",
                 "sample2.recal.regions.bed.gz:md5,fb0efeba20ea272b7b709cf65246689e",
-                "sample2.recal.regions.bed.gz.csi:md5,e8452848671e9e5c147ff4cceee944af",
+                "sample2.recal.regions.bed.gz.csi:md5,e95efa0e55e9f9699b48e6a661d5e1e4",
                 "sample2.mutect2.FILTER.summary:md5,08f06620a8dcc70115bdde9137a91008",
                 "sample2.mutect2.TsTv.count:md5,0d59dcbdb127be60909111958ff7b5f5",
                 "sample2.mutect2.vcf.gz.stats:md5,76f749c53212d72e98801f6030fbf8a6"
@@ -816,10 +820,10 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
+        "timestamp": "2026-07-27T10:33:25.493676415",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T23:30:58.725141411"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_sentieon_dnascope.nf.test.snap b/tests/variant_calling_sentieon_dnascope.nf.test.snap
index 1440d01f1a..6aae5c5a95 100644
--- a/tests/variant_calling_sentieon_dnascope.nf.test.snap
+++ b/tests/variant_calling_sentieon_dnascope.nf.test.snap
@@ -13,10 +13,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DNASCOPE": {
                     "sentieon": "202503.02"
@@ -166,7 +167,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.dnascope.unfiltered.bcftools_stats.txt:md5,e7e014ba2764fca171a3291c26cca34c",
@@ -174,7 +175,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.dnascope.unfiltered.FILTER.summary:md5,87a84b5f8ac3d3cbeeef7d60afcdbfe7",
                 "test.dnascope.unfiltered.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -185,11 +186,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:38:52.499416464",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T12:35:10.846318732"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dnascope": {
         "content": [
@@ -205,10 +206,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DNAMODELAPPLY": {
                     "sentieon": "202503.02"
@@ -363,7 +365,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.dnascope.filtered.bcftools_stats.txt:md5,af2789c5de131bbd90b133b9382f506f",
@@ -371,7 +373,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.dnascope.filtered.FILTER.summary:md5,e67b24d296810a075378e5864bcea0fa",
                 "test.dnascope.filtered.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
             ],
@@ -383,11 +385,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:34:52.637438538",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T11:44:42.944302645"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_dnascope --joint_germline --sentieon_dnascope_emit_mode gvcf": {
         "content": [
@@ -409,10 +411,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_DNASCOPE": {
                     "sentieon": "202503.02"
@@ -568,7 +571,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "joint_germline.bcftools_stats.txt:md5,936ca7dc4a8d81cb73a9446057ec4b24",
@@ -576,7 +579,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "joint_germline.FILTER.summary:md5,b489034e69de07875cbf0f5548b4f55e",
                 "joint_germline.TsTv.count:md5,803d74e40f7716202bae2a3a81c1ddfc"
             ],
@@ -590,10 +593,10 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
+        "timestamp": "2026-07-27T11:36:02.196958675",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T16:52:44.930404505"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
index c50574d646..f861d9b5b2 100644
--- a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
@@ -19,10 +19,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_APPLYVARCAL_INDEL": {
                     "sentieon": "202503.02"
@@ -192,7 +193,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "joint_germline_recalibrated_indel.bcftools_stats.txt:md5,f87581b827c876e433e51e39342379ca",
@@ -200,7 +201,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "joint_germline_recalibrated_indel.FILTER.summary:md5,7e04c3bed9ecbb73aa3c02c2b6b46089",
                 "joint_germline_recalibrated_indel.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc"
             ],
@@ -215,11 +216,11 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
+        "timestamp": "2026-07-27T11:36:08.094597613",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-23T20:44:13.797239672"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_haplotyper --no_intervals": {
         "content": [
@@ -235,10 +236,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_HAPLOTYPER": {
                     "sentieon": "202503.02"
@@ -389,7 +391,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotyper.filtered.bcftools_stats.txt:md5,f4863054921218b7821858f5836029e4",
@@ -397,7 +399,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.haplotyper.filtered.FILTER.summary:md5,d501a93356f3c91c743f51104e24514a",
                 "test.haplotyper.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc"
             ],
@@ -409,11 +411,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:36:40.990413214",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T14:35:09.570055964"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_haplotyper --skip_tools haplotyper_filter": {
         "content": [
@@ -429,10 +431,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_HAPLOTYPER": {
                     "sentieon": "202503.02"
@@ -582,7 +585,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotyper.unfiltered.bcftools_stats.txt:md5,69874476b2830366fff35b242dbfdfa5",
@@ -590,7 +593,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.haplotyper.unfiltered.FILTER.summary:md5,01b3d10464a3ac86f90ee82cdda23f68",
                 "test.haplotyper.unfiltered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc"
             ],
@@ -601,11 +604,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:36:30.915587402",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T14:41:55.821821171"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools sentieon_haplotyper": {
         "content": [
@@ -627,10 +630,11 @@
                     "gatk4": "4.6.2.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SENTIEON_HAPLOTYPER": {
                     "sentieon": "202503.02"
@@ -782,7 +786,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
                 "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,06abce459a3e3192252b6196ae3b189e",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
                 "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
                 "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
                 "test.haplotyper.filtered.bcftools_stats.txt:md5,f4863054921218b7821858f5836029e4",
@@ -790,7 +794,7 @@
                 "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
                 "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
                 "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
-                "test.recal.regions.bed.gz.csi:md5,b3716e5cd1744610e69c29bd4ffad259",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
                 "test.haplotyper.filtered.FILTER.summary:md5,d501a93356f3c91c743f51104e24514a",
                 "test.haplotyper.filtered.TsTv.count:md5,89562fef808b5c3db629682d36fd86fc"
             ],
@@ -802,10 +806,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T11:36:24.959312656",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T14:24:33.689599405"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_strelka.nf.test.snap b/tests/variant_calling_strelka.nf.test.snap
index 11ec9a4495..5a8093d26e 100644
--- a/tests/variant_calling_strelka.nf.test.snap
+++ b/tests/variant_calling_strelka.nf.test.snap
@@ -10,10 +10,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -191,7 +192,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.strelka.variants.bcftools_stats.txt:md5,2c40e667f6ee75761fe6c3b33dda0679",
@@ -201,12 +202,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.strelka.variants.FILTER.summary:md5,fef8aeadd3b0f3b8c040c0da03bf1cbd",
                 "sample3.strelka.variants.TsTv.count:md5,c5b7a8eda2526d899098439ae4c06a49",
                 "sample4_vs_sample3.strelka.somatic_indels.FILTER.summary:md5,30a45e2bc87f40c89388032cbf75ec65",
@@ -224,11 +225,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:30:09.214764374",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:48:23.853303187"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools strelka germline": {
         "content": [
@@ -241,10 +242,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -392,7 +394,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
@@ -400,7 +402,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.strelka.variants.FILTER.summary:md5,fef8aeadd3b0f3b8c040c0da03bf1cbd",
                 "sample1.strelka.variants.TsTv.count:md5,c5b7a8eda2526d899098439ae4c06a49"
             ],
@@ -412,11 +414,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:33:17.232108325",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:45:18.036242508"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools strelka --only_paired_variant_calling": {
         "content": [
@@ -429,10 +431,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -626,7 +629,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,ad0637d55d7025330f2f6cb7f9680e64",
                 "mosdepth_perchrom.txt:md5,73ef9a077df1887f9021a581fbf207bc",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,b446a47b182d93a9e7e74f5f7c8d41c2",
+                "samtools-stats-dp.txt:md5,a6501ad36c9d1e9b97878e69f072d63c",
                 "samtools_alignment_plot.txt:md5,7138a2d29f515993e1df8d745e27b757",
                 "samtools_insert_size.txt:md5,e0c5f4ebb18a0e6bd437f0b919725b39",
                 "sample1.strelka.variants.bcftools_stats.txt:md5,bcb50cfcfefdce0d203aff1d054d9f24",
@@ -636,22 +639,22 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample2.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.recal.mosdepth.global.dist.txt:md5,d9a4dd6429560b2b647da346050766c5",
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample1.strelka.variants.FILTER.summary:md5,fef8aeadd3b0f3b8c040c0da03bf1cbd",
                 "sample1.strelka.variants.TsTv.count:md5,c5b7a8eda2526d899098439ae4c06a49",
                 "sample4_vs_sample3.strelka.somatic_indels.FILTER.summary:md5,30a45e2bc87f40c89388032cbf75ec65",
@@ -669,11 +672,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:37:04.502091857",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:43:48.55687233"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools strelka --no_intervals germline": {
         "content": [
@@ -683,10 +686,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -833,7 +837,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.strelka.variants.bcftools_stats.txt:md5,198d97ab341f78b4c33ee345ebdfe5e2",
@@ -841,7 +845,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.strelka.variants.FILTER.summary:md5,8697a0a983314e98b99b5f6038af65f6",
                 "sample1.strelka.variants.TsTv.count:md5,1481854d2a765f5641856ecf95ca4097"
             ],
@@ -853,11 +857,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:30:57.796189985",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:46:48.703134861"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools strelka --no_intervals somatic": {
         "content": [
@@ -867,10 +871,11 @@
                     "bcftools": "1.23.1"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -1047,7 +1052,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.strelka.variants.bcftools_stats.txt:md5,9a87ae32f97121c0e033043cd6605f67",
@@ -1057,12 +1062,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.strelka.variants.FILTER.summary:md5,8697a0a983314e98b99b5f6038af65f6",
                 "sample3.strelka.variants.TsTv.count:md5,1481854d2a765f5641856ecf95ca4097",
                 "sample4_vs_sample3.strelka.somatic_indels.FILTER.summary:md5,30a45e2bc87f40c89388032cbf75ec65",
@@ -1080,10 +1085,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:35:17.803996008",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:49:57.22542474"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_strelka_bp.nf.test.snap b/tests/variant_calling_strelka_bp.nf.test.snap
index a24394f377..9ffbc319f1 100644
--- a/tests/variant_calling_strelka_bp.nf.test.snap
+++ b/tests/variant_calling_strelka_bp.nf.test.snap
@@ -13,10 +13,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -220,7 +221,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
@@ -233,12 +234,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample3.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample4_vs_sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -265,11 +266,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:32:41.117980259",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:53:32.414589191"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools manta,strelka somatic": {
         "content": [
@@ -288,10 +289,11 @@
                     "manta": "1.6.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "STRELKA_SINGLE": {
                     "strelka": "2.9.10"
@@ -496,7 +498,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.manta.diploid_sv.bcftools_stats.txt:md5,5aafdb8e25b73849c4dae1aace2154ca",
@@ -509,12 +511,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample3.manta.diploid_sv.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample4_vs_sample3.manta.diploid_sv.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -541,10 +543,10 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-27T10:35:43.999417751",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:51:45.217803492"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_tiddit.nf.test.snap b/tests/variant_calling_tiddit.nf.test.snap
index 2b872c1619..09bb83af3f 100644
--- a/tests/variant_calling_tiddit.nf.test.snap
+++ b/tests/variant_calling_tiddit.nf.test.snap
@@ -10,10 +10,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -138,7 +139,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,edd8dfc8023e629e476e01c3a1448d30",
                 "mosdepth_perchrom.txt:md5,49fd828d21d79a5c9430cdb30d7f0126",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,41d36c1a8413d565788a1a364b467606",
+                "samtools-stats-dp.txt:md5,5b881919a10bdc8661d05845dead0a98",
                 "samtools_alignment_plot.txt:md5,35f8fd2a557568b2237193f46afbab5c",
                 "samtools_insert_size.txt:md5,be7a60f43f0ca5238674cbf0f9d48917",
                 "sample1.tiddit.bcftools_stats.txt:md5,ce8d4ce1c8fde22cf1557603caa1c4e9",
@@ -146,7 +147,7 @@
                 "sample1.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample1.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample1.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample1.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample1.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample1.tiddit.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample1.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -157,10 +158,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-20T09:36:34.643829502",
+        "timestamp": "2026-07-27T10:29:34.219316164",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools tiddit tumoronly": {
@@ -174,10 +175,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
                     "bgzip": "1.21",
@@ -302,7 +304,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,8398d1127a10d0c002831ddedfb9713b",
                 "mosdepth_perchrom.txt:md5,ff38f1e35ce12244c751921db673b23c",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,6618ece77181051a58275f504f67ea5b",
+                "samtools-stats-dp.txt:md5,42facbec7ea07232de8dc0369f0da065",
                 "samtools_alignment_plot.txt:md5,6136e5e1d072f166f280fb79424c392f",
                 "samtools_insert_size.txt:md5,5cb5779e1a7baf077b529af8c427147c",
                 "sample2.tiddit.bcftools_stats.txt:md5,f49030d75a44dde7a9d068af09e89c52",
@@ -310,7 +312,7 @@
                 "sample2.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample2.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample2.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample2.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample2.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample2.tiddit.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample2.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a"
             ],
@@ -321,10 +323,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-20T09:39:22.408315557",
+        "timestamp": "2026-07-27T10:32:32.25435422",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools tiddit somatic": {
@@ -338,10 +340,11 @@
                     "gawk": "5.3.0"
                 },
                 "MOSDEPTH": {
-                    "mosdepth": "0.3.10"
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
                 },
                 "SAMTOOLS_STATS": {
-                    "samtools": 1.21
+                    "samtools": "1.24"
                 },
                 "SVDB_MERGE": {
                     "bcftools": "1.23",
@@ -491,7 +494,7 @@
                 "mosdepth-cumcoverage-dist-id.txt:md5,0cc87c596a0fc30ebb676c4587e986de",
                 "mosdepth_perchrom.txt:md5,336d786b273c4d4e714d51c44207ff56",
                 "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
-                "samtools-stats-dp.txt:md5,e0a8d8867064083908a8ca1ea782d7ac",
+                "samtools-stats-dp.txt:md5,bf6d30315fd7608b97b2a4eb1dc71fdc",
                 "samtools_alignment_plot.txt:md5,f4b1a7cef760291172144a8614b4a1cd",
                 "samtools_insert_size.txt:md5,8fa108dcbe0da42e9425dd125fe22905",
                 "sample3.tiddit.bcftools_stats.txt:md5,981a4170dd5a6bd6e5f51bbb169f8a17",
@@ -500,12 +503,12 @@
                 "sample3.recal.mosdepth.region.dist.txt:md5,1f3dab381958e08eb00f7c5e1135f677",
                 "sample3.recal.mosdepth.summary.txt:md5,d7676e7c1de851b0ee5185d21096123b",
                 "sample3.recal.regions.bed.gz:md5,6edeb8f7041a4403cb73651744b5bc82",
-                "sample3.recal.regions.bed.gz.csi:md5,5fc6f880df27ca754ab229f0ccad2aea",
+                "sample3.recal.regions.bed.gz.csi:md5,9fa6b12aa02f0f0a673b1fbe19d6fb75",
                 "sample4.recal.mosdepth.global.dist.txt:md5,53f9ae9ab5002ffba340fa8cef7d70e4",
                 "sample4.recal.mosdepth.region.dist.txt:md5,17600d21ac453506c52249cf435ad8ea",
                 "sample4.recal.mosdepth.summary.txt:md5,7141030385af1f653718c9e0c9a5be80",
                 "sample4.recal.regions.bed.gz:md5,c680c5d75f0cea068e3f917f4cf9bf52",
-                "sample4.recal.regions.bed.gz.csi:md5,68b7a9a98053b1122bdca68a1e1c87dd",
+                "sample4.recal.regions.bed.gz.csi:md5,569288ccaecad1a91b1310d0e981a497",
                 "sample3.tiddit.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
                 "sample3.tiddit.TsTv.count:md5,fa27f678965b7cba6a92efcd039f802a",
                 "sample4_vs_sample3.tiddit_sv_merge.FILTER.summary:md5,1ce42d34e4ae919afb519efc99146423",
@@ -521,10 +524,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-05-20T09:38:11.731089821",
+        "timestamp": "2026-07-27T10:24:40.00162687",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.1"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index eef91cfbc7..da01b6bf97 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -168,7 +168,6 @@ workflow SAREK {
             true,
         )
 
-
         two_fastq_gz_from_spring = r1_fastq_gz_from_spring.fastq.join(r2_fastq_gz_from_spring.fastq).map { meta, fastq_1, fastq_2 -> [meta, [fastq_1, fastq_2]] }
 
         two_fastq_gz_from_spring = two_fastq_gz_from_spring.map { meta, files -> addReadgroupToMeta(meta, files) }
@@ -180,13 +179,11 @@ workflow SAREK {
         interleave_input = false
         CONVERT_FASTQ_INPUT(
             input_sample_type.bam,
-            [[id: "fasta"], []],
-            [[id: 'null'], []],
+            fasta,
+            fasta_fai,
             interleave_input,
         )
 
-        versions = versions.mix(CONVERT_FASTQ_INPUT.out.versions)
-
         // Gather fastq (inputed or converted)
         // Theorically this could work on mixed input (fastq for one sample and bam for another)
         // But not sure how to handle that with the samplesheet
@@ -228,7 +225,6 @@ workflow SAREK {
 
             // Gather used softwares versions
             reports = reports.mix(FASTQ_PREPROCESS_PARABRICKS.out.reports)
-            versions = versions.mix(FASTQ_PREPROCESS_PARABRICKS.out.versions)
         }
         else {
             // PREPROCESSING
@@ -292,7 +288,7 @@ workflow SAREK {
             }
 
             // convert cram files
-            CRAM_TO_BAM(cram_variant_calling_status_tmp.cram, fasta, fasta_fai)
+            CRAM_TO_BAM(cram_variant_calling_status_tmp.cram, fasta.combine(fasta_fai).map { meta, fasta_, _meta_fai, fai -> [ meta, fasta_, fai ] }.collect())
 
             // gather all bam files
             bam_variant_calling = CRAM_TO_BAM.out.bam
@@ -301,8 +297,6 @@ workflow SAREK {
                 .map { meta, bam, bai ->
                     [meta + [data_type: 'bam'], bam, bai]
                 }
-
-            versions = versions.mix(CRAM_TO_BAM.out.versions)
         }
 
         // Logic to separate germline samples, tumor samples with no matched normal, and combine tumor-normal pairs

From 62edfac9d49d22b1139b24c709660562df161795 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Mon, 27 Jul 2026 21:37:47 +0200
Subject: [PATCH 18/27] chore(modules): migrate QC/coverage modules to versions
 topic channel (#2242)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates QC / coverage modules to the `versions` topic channel.
**Stacked on #2241** (base: `topic/samtools`).

### Changes
- Updates `fastqc`, `ngscheckmate/ncm`, `goleft/indexcov`,
`msisensor2/msi`, `msisensorpro/{msisomatic,scan}`, `ascat` to their
topic-channel versions (fastqc + ngscheckmate were already migrated on
dev).
- Removes the corresponding `.out.versions` wiring.
Test snapshots need regenerating in CI.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 .github/actions/nf-test/action.yml            |   7 -
 CHANGELOG.md                                  |   1 +
 modules.json                                  |  10 +-
 modules/nf-core/ascat/main.nf                 | 101 +++++------
 modules/nf-core/ascat/meta.yml                | 170 ++++++++++++------
 modules/nf-core/goleft/indexcov/main.nf       |  51 +++---
 modules/nf-core/goleft/indexcov/meta.yml      |  94 +++++++---
 modules/nf-core/msisensor2/msi/main.nf        |  26 +--
 modules/nf-core/msisensor2/msi/meta.yml       |  29 ++-
 .../nf-core/msisensorpro/msisomatic/main.nf   |  16 +-
 .../nf-core/msisensorpro/msisomatic/meta.yml  |  44 +++--
 modules/nf-core/msisensorpro/scan/main.nf     |  16 +-
 modules/nf-core/msisensorpro/scan/meta.yml    |  40 +++--
 subworkflows/local/bam_markduplicates/main.nf |   6 -
 .../local/bam_markduplicates_spark/main.nf    |   5 -
 subworkflows/local/bam_sentieon_dedup/main.nf |   6 -
 .../bam_variant_calling_indexcov/main.nf      |   3 -
 .../bam_variant_calling_somatic_all/main.nf   |   2 -
 .../bam_variant_calling_somatic_ascat/main.nf |   7 -
 .../main.nf                                   |   1 -
 .../local/cram_qc_mosdepth_samtools/main.nf   |   2 -
 subworkflows/local/cram_sampleqc/main.nf      |   5 -
 .../local/fastq_preprocess_gatk/main.nf       |  23 ---
 subworkflows/local/prepare_genome/main.nf     |   1 -
 tests/variant_calling_ascat.nf.test.snap      |   4 +-
 tests/variant_calling_msisensor2.nf.test.snap |   2 +-
 workflows/sarek.nf                            |   4 +-
 27 files changed, 352 insertions(+), 324 deletions(-)

diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
index 341139f2f3..434f7b247e 100644
--- a/.github/actions/nf-test/action.yml
+++ b/.github/actions/nf-test/action.yml
@@ -74,14 +74,7 @@ runs:
         NFT_WORKDIR: ${{ env.NFT_WORKDIR }}
         SENTIEON_LICSRVR_IP: ${{ env.SENTIEON_LICSRVR_IP }}
         SENTIEON_AUTH_MECH: "GitHub Actions - token"
-        # Use nf-test's native differ instead of pdiff: pdiff wraps the snapshot
-        # diff to the terminal width and truncates md5 checksums in the CI log.
-        # The native differ prints full-length values on one line (scrapeable).
-        NFT_DIFF: diff
       run: |
-        # setup-nf-test exports NFT_DIFF_ARGS (pdiff flags) into the env; those
-        # flags suppress the native differ's output entirely, so drop them here.
-        unset NFT_DIFF_ARGS
         # In update-snapshot mode, regenerate snapshots over ALL tagged tests
         # (drop --changed-since, which is only meaningful for PR/push diffs).
         CHANGED_SINCE="--changed-since HEAD^"
diff --git a/CHANGELOG.md b/CHANGELOG.md
index cbac014e25..e5d006f4e3 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,6 +22,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2239](https://github.com/nf-core/sarek/pull/2239) - Migrate alignment/UMI/utility modules (`bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`, `gunzip`, `untar`, `unzip`, `spring`) to the versions topic channel (fastp 0.24.0 → 1.1.0)
 - [#2240](https://github.com/nf-core/sarek/pull/2240) - Migrate variant-calling modules (`freebayes`, `strelka`, `manta`, `tiddit`, `lofreq`, `svdb`, `vcflib`, `vcftools`) to the versions topic channel
 - [#2241](https://github.com/nf-core/sarek/pull/2241) - Migrate `samtools/*` and `mosdepth` modules to the versions topic channel
+- [#2242](https://github.com/nf-core/sarek/pull/2242) - Migrate QC/coverage modules (`fastqc`, `ngscheckmate`, `goleft`, `msisensor2`, `msisensorpro`, `ascat`) to the versions topic channel
 
 ### Fixed
 
diff --git a/modules.json b/modules.json
index fc37c5232f..d8ee6235ab 100644
--- a/modules.json
+++ b/modules.json
@@ -7,7 +7,7 @@
                 "nf-core": {
                     "ascat": {
                         "branch": "master",
-                        "git_sha": "edd5dd7ef8d99cc059dcdff2de08d936838b8ca4",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "bbmap/bbsplit": {
@@ -338,7 +338,7 @@
                     },
                     "goleft/indexcov": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "268dd690f53fd7f7bb524648ef1144a9b4e963eb",
                         "installed_by": ["modules"]
                     },
                     "gunzip": {
@@ -373,17 +373,17 @@
                     },
                     "msisensor2/msi": {
                         "branch": "master",
-                        "git_sha": "d96d6f176de5729d21c3b33a610b486f7ef7eac9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "msisensorpro/msisomatic": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "8f37155cb42259efed9e9aef24f7d1ba66ea983f",
                         "installed_by": ["modules"]
                     },
                     "msisensorpro/scan": {
                         "branch": "master",
-                        "git_sha": "41dfa3f7c0ffabb96a6a813fe321c6d1cc5b6e46",
+                        "git_sha": "8f37155cb42259efed9e9aef24f7d1ba66ea983f",
                         "installed_by": ["modules"]
                     },
                     "multiqc": {
diff --git a/modules/nf-core/ascat/main.nf b/modules/nf-core/ascat/main.nf
index b18948f525..4dac1f90f4 100644
--- a/modules/nf-core/ascat/main.nf
+++ b/modules/nf-core/ascat/main.nf
@@ -1,65 +1,70 @@
 process ASCAT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4c/4cf02c7911ee5e974ce7db978810770efbd8d872ff5ab3462d2a11bcf022fab5/data':
-        'community.wave.seqera.io/library/ascat_cancerit-allelecount:c3e8749fa4af0e99' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/4c/4cf02c7911ee5e974ce7db978810770efbd8d872ff5ab3462d2a11bcf022fab5/data'
+        : 'community.wave.seqera.io/library/ascat_cancerit-allelecount:c3e8749fa4af0e99'}"
 
     input:
     tuple val(meta), path(input_normal), path(index_normal), path(input_tumor), path(index_tumor)
-    path(allele_files)
-    path(loci_files)
-    path(bed_file)  // optional
-    path(fasta)     // optional
-    path(gc_file)   // optional
-    path(rt_file)   // optional
+    path allele_files
+    path loci_files
+    path bed_file
+    path fasta
+    path gc_file
+    path rt_file
 
     output:
     tuple val(meta), path("*alleleFrequencies_chr*.txt"), emit: allelefreqs
-    tuple val(meta), path("*BAF.txt")                   , emit: bafs
-    tuple val(meta), path("*cnvs.txt")                  , emit: cnvs
-    tuple val(meta), path("*LogR.txt")                  , emit: logrs
-    tuple val(meta), path("*metrics.txt")               , emit: metrics
-    tuple val(meta), path("*png")                       , emit: png
-    tuple val(meta), path("*purityploidy.txt")          , emit: purityploidy
-    tuple val(meta), path("*segments.txt")              , emit: segments
-    path "versions.yml"                                 , emit: versions
+    tuple val(meta), path("*BAF.txt"),                    emit: bafs
+    tuple val(meta), path("*cnvs.txt"),                   emit: cnvs
+    tuple val(meta), path("*LogR.txt"),                   emit: logrs
+    tuple val(meta), path("*metrics.txt"),                emit: metrics
+    tuple val(meta), path("*png"),                        emit: png
+    tuple val(meta), path("*purityploidy.txt"),           emit: purityploidy
+    tuple val(meta), path("*segments.txt"),               emit: segments
+    tuple val("${task.process}"), val('bioconductor-ascat'), eval('Rscript -e "library(ASCAT); cat(as.character(packageVersion(\'ASCAT\')))"'), topic: versions, emit: versions_ascat
+    tuple val("${task.process}"), val('alleleCounter'), eval("alleleCounter --version"), topic: versions, emit: versions_allelecounter
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    def args           = task.ext.args        ?: ''
-    def prefix         = task.ext.prefix      ?: "${meta.id}"
-    def gender         = args.gender          ?  "${args.gender}"        : "NULL"
-    def genomeVersion  = args.genomeVersion   ?  "${args.genomeVersion}" : "NULL"
-    def purity         = args.purity          ?  "${args.purity}"        : "NULL"
-    def ploidy         = args.ploidy          ?  "${args.ploidy}"        : "NULL"
-    def gc_input       = gc_file              ?  "${gc_file}"            : "NULL"
-    def rt_input       = rt_file              ?  "${rt_file}"            : "NULL"
-
-    def minCounts_arg                    = args.minCounts                      ?  ", minCounts = ${args.minCounts}"         : ""
-    def bed_file_arg                     = bed_file                            ?  ", BED_file = '${bed_file}'"              : ""
-    def chrom_names_arg                  = args.chrom_names                    ?  ", chrom_names = ${args.chrom_names}"     : ""
-    def min_base_qual_arg                = args.min_base_qual                  ?  ", min_base_qual = ${args.min_base_qual}" : ""
-    def min_map_qual_arg                 = args.min_map_qual                   ?  ", min_map_qual = ${args.min_map_qual}"   : ""
-    def skip_allele_counting_tumour_arg  = args.skip_allele_counting_tumour    ?  ", skip_allele_counting_tumour = ${args.skip_allele_counting_tumour}"       : ""
-    def skip_allele_counting_normal_arg  = args.skip_allele_counting_normal    ?  ", skip_allele_counting_normal = ${args.skip_allele_counting_normal}"       : ""
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
 
-    if(args.additional_allelecounter_flags && fasta) {
+    def gender        = args.gender        ? "${args.gender}"        : "NULL"
+    def genomeVersion = args.genomeVersion ? "${args.genomeVersion}" : "NULL"
+    def purity        = args.purity        ? "${args.purity}"        : "NULL"
+    def ploidy        = args.ploidy        ? "${args.ploidy}"        : "NULL"
+    def gc_input      = gc_file            ? "${gc_file}"            : "NULL"
+    def rt_input      = rt_file            ? "${rt_file}"            : "NULL"
+
+    def minCounts_arg                   = args.minCounts                   ? ", minCounts = ${args.minCounts}"                                     : ""
+    def bed_file_arg                    = bed_file                         ? ", BED_file = '${bed_file}'"                                          : ""
+    def chrom_names_arg                 = args.chrom_names                 ? ", chrom_names = ${args.chrom_names}"                                 : ""
+    def min_base_qual_arg               = args.min_base_qual               ? ", min_base_qual = ${args.min_base_qual}"                             : ""
+    def min_map_qual_arg                = args.min_map_qual                ? ", min_map_qual = ${args.min_map_qual}"                               : ""
+    def skip_allele_counting_tumour_arg = args.skip_allele_counting_tumour ? ", skip_allele_counting_tumour = ${args.skip_allele_counting_tumour}" : ""
+    def skip_allele_counting_normal_arg = args.skip_allele_counting_normal ? ", skip_allele_counting_normal = ${args.skip_allele_counting_normal}" : ""
+
+    if (args.additional_allelecounter_flags && fasta) {
         additional_allelecounter_arg = ", additional_allelecounter_flags = \"${args.additional_allelecounter_flags} -r ${fasta}\" "
-    } else if (args.additional_allelecounter_flags ) {
+    }
+    else if (args.additional_allelecounter_flags) {
         additional_allelecounter_arg = ", additional_allelecounter_flags = \"${args.additional_allelecounter_flags}\" "
-    } else if (fasta) {
+    }
+    else if (fasta) {
         additional_allelecounter_arg = ", additional_allelecounter_flags = '-r \"${fasta}\"'"
-    } else {
+    }
+    else {
         additional_allelecounter_arg = ""
     }
 
     """
-    #!/usr/bin/env Rscript
+    Rscript - <<'EOF'
     library(RColorBrewer)
     library(ASCAT)
     options(bitmapType='cairo')
@@ -213,15 +218,7 @@ process ASCAT {
     write.table(summary, file=paste0("${prefix}",".purityploidy.txt"), sep="\t", quote=F, row.names=F, col.names=T)
 
     write.table(QC, file=paste0("${prefix}", ".metrics.txt"), sep="\t", quote=F, row.names=F)
-
-    # Version export
-    f <- file("versions.yml","w")
-    alleleCounter_version = system(paste("alleleCounter --version"), intern = T)
-    ascat_version = as.character(packageVersion('ASCAT'))
-    writeLines(paste0('"', "${task.process}", '"', ":"), f)
-    writeLines(paste("    ascat:", ascat_version), f)
-    writeLines(paste("    alleleCounter:", alleleCounter_version), f)
-    close(f)
+    EOF
     """
 
     stub:
@@ -245,13 +242,5 @@ process ASCAT {
     touch ${prefix}.tumour_normalLogR.txt
     touch ${prefix}.tumour_tumourBAF.txt
     touch ${prefix}.tumour_tumourLogR.txt
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        bioconductor-ascat: \$(Rscript -e "library(ASCAT); cat(as.character(packageVersion('ASCAT')))")
-        alleleCounter: \$(alleleCounter --version)
-    END_VERSIONS
     """
-
-
 }
diff --git a/modules/nf-core/ascat/meta.yml b/modules/nf-core/ascat/meta.yml
index db7c92926a..8aebd0bc76 100644
--- a/modules/nf-core/ascat/meta.yml
+++ b/modules/nf-core/ascat/meta.yml
@@ -6,17 +6,18 @@ keywords:
   - cram
 tools:
   - ascat:
-      description: ASCAT is a method to derive copy number profiles of tumour cells,
-        accounting for normal cell admixture and tumour aneuploidy. ASCAT infers tumour
-        purity (the fraction of tumour cells) and ploidy (the amount of DNA per tumour
-        cell), expressed as multiples of haploid genomes from SNP array or massively
-        parallel sequencing data, and calculates whole-genome allele-specific copy number
-        profiles (the number of copies of both parental alleles for all SNP loci across
-        the genome).
+      description: ASCAT is a method to derive copy number profiles of tumour
+        cells, accounting for normal cell admixture and tumour aneuploidy. ASCAT
+        infers tumour purity (the fraction of tumour cells) and ploidy (the amount
+        of DNA per tumour cell), expressed as multiples of haploid genomes from
+        SNP array or massively parallel sequencing data, and calculates
+        whole-genome allele-specific copy number profiles (the number of copies of
+        both parental alleles for all SNP loci across the genome).
       documentation: https://github.com/VanLoo-lab/ascat/tree/master/man
       tool_dev_url: https://github.com/VanLoo-lab/ascat
       doi: "10.1093/bioinformatics/btaa538"
-      licence: ["GPL v3"]
+      licence:
+        - "GPL v3"
       identifier: biotools:ascat
 input:
   - - meta:
@@ -26,51 +27,63 @@ input:
           e.g. [ id:'test', single_end:false ]
     - input_normal:
         type: file
-        description: BAM/CRAM file, must adhere to chr1, chr2, ...chrX notation For
-          modifying chromosome notation in bam files please follow
+        description: BAM/CRAM file, must adhere to chr1, chr2, ...chrX notation
+          For modifying chromosome notation in bam files please follow
           https://josephcckuo.wordpress.com/2016/11/17/modify-chromosome-notation-in-bam-file/.
         pattern: "*.{bam,cram}"
+        ontologies: []
     - index_normal:
         type: file
         description: index for normal_bam/cram
         pattern: "*.{bai,crai}"
+        ontologies: []
     - input_tumor:
         type: file
         description: BAM/CRAM file, must adhere to chr1, chr2, ...chrX notation
         pattern: "*.{bam,cram}"
+        ontologies: []
     - index_tumor:
         type: file
         description: index for tumor_bam/cram
         pattern: "*.{bai,crai}"
-  - - allele_files:
-        type: file
-        description: allele files for ASCAT WGS. Can be downloaded here https://github.com/VanLoo-lab/ascat/tree/master/ReferenceFiles/WGS
-  - - loci_files:
-        type: file
-        description: loci files for ASCAT WGS. Loci files without chromosome notation
-          can be downloaded here https://github.com/VanLoo-lab/ascat/tree/master/ReferenceFiles/WGS
-          Make sure the chromosome notation matches the bam/cram input files. To add
-          the chromosome notation to loci files (hg19/hg38) if necessary, you can run
-          this command `if [[ $(samtools view <your_bam_file.bam> | head -n1 | cut -f3)\"
-          == *\"chr\"* ]]; then for i in {1..22} X; do sed -i 's/^/chr/' G1000_loci_hg19_chr_${i}.txt;
-          done; fi`
-  - - bed_file:
-        type: file
-        description: Bed file for ASCAT WES (optional, but recommended for WES)
-  - - fasta:
-        type: file
-        description: Reference fasta file (optional)
-  - - gc_file:
-        type: file
-        description: GC correction file (optional) - Used to do logR correction of the
-          tumour sample(s) with genomic GC content
-  - - rt_file:
-        type: file
-        description: replication timing correction file (optional, provide only in combination
-          with gc_file)
+        ontologies: []
+  - allele_files:
+      type: file
+      description: allele files for ASCAT WGS. Can be downloaded here
+        https://github.com/VanLoo-lab/ascat/tree/master/ReferenceFiles/WGS
+      ontologies: []
+  - loci_files:
+      type: file
+      description: loci files for ASCAT WGS. Loci files without chromosome
+        notation can be downloaded here
+        https://github.com/VanLoo-lab/ascat/tree/master/ReferenceFiles/WGS Make
+        sure the chromosome notation matches the bam/cram input files. To add the
+        chromosome notation to loci files (hg19/hg38) if necessary, you can run
+        this command `if [[ $(samtools view <your_bam_file.bam> | head -n1 | cut
+        -f3)\" == *\"chr\"* ]]; then for i in {1..22} X; do sed -i 's/^/chr/'
+        G1000_loci_hg19_chr_${i}.txt; done; fi`
+      ontologies: []
+  - bed_file:
+      type: file
+      description: Bed file for ASCAT WES (optional, but recommended for WES)
+      ontologies: []
+  - fasta:
+      type: file
+      description: Reference fasta file (optional)
+      ontologies: []
+  - gc_file:
+      type: file
+      description: GC correction file (optional) - Used to do logR correction of
+        the tumour sample(s) with genomic GC content
+      ontologies: []
+  - rt_file:
+      type: file
+      description: replication timing correction file (optional, provide only in
+        combination with gc_file)
+      ontologies: []
 output:
-  - allelefreqs:
-      - meta:
+  allelefreqs:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -79,8 +92,9 @@ output:
           type: file
           description: Files containing allee frequencies per chromosome
           pattern: "*{alleleFrequencies_chr*.txt}"
-  - bafs:
-      - meta:
+          ontologies: []
+  bafs:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -88,8 +102,9 @@ output:
       - "*BAF.txt":
           type: file
           description: BAF file
-  - cnvs:
-      - meta:
+          ontologies: []
+  cnvs:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -97,8 +112,9 @@ output:
       - "*cnvs.txt":
           type: file
           description: CNV file
-  - logrs:
-      - meta:
+          ontologies: []
+  logrs:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -106,8 +122,9 @@ output:
       - "*LogR.txt":
           type: file
           description: LogR file
-  - metrics:
-      - meta:
+          ontologies: []
+  metrics:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -116,8 +133,9 @@ output:
           type: file
           description: File containing quality metrics
           pattern: "*.{metrics.txt}"
-  - png:
-      - meta:
+          ontologies: []
+  png:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -126,8 +144,9 @@ output:
           type: file
           description: ASCAT plots
           pattern: "*.{png}"
-  - purityploidy:
-      - meta:
+          ontologies: []
+  purityploidy:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -136,8 +155,9 @@ output:
           type: file
           description: File with purity and ploidy data
           pattern: "*.{purityploidy.txt}"
-  - segments:
-      - meta:
+          ontologies: []
+  segments:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -146,11 +166,47 @@ output:
           type: file
           description: File with segments data
           pattern: "*.{segments.txt}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_ascat:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bioconductor-ascat:
+          type: string
+          description: The name of the tool
+      - Rscript -e "library(ASCAT); cat(as.character(packageVersion('ASCAT')))":
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_allelecounter:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - alleleCounter:
+          type: string
+          description: The name of the tool
+      - alleleCounter --version:
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - bioconductor-ascat:
+          type: string
+          description: The name of the tool
+      - Rscript -e "library(ASCAT); cat(as.character(packageVersion('ASCAT')))":
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - alleleCounter:
+          type: string
+          description: The name of the tool
+      - alleleCounter --version:
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@aasNGC"
   - "@lassefolkersen"
diff --git a/modules/nf-core/goleft/indexcov/main.nf b/modules/nf-core/goleft/indexcov/main.nf
index 5d0ed5dfb0..b9402734e4 100644
--- a/modules/nf-core/goleft/indexcov/main.nf
+++ b/modules/nf-core/goleft/indexcov/main.nf
@@ -3,23 +3,24 @@ process GOLEFT_INDEXCOV {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/goleft:0.2.4--h9ee0642_1':
-        'biocontainers/goleft:0.2.4--h9ee0642_1' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/goleft:0.2.4--h9ee0642_1'
+        : 'quay.io/biocontainers/goleft:0.2.4--h9ee0642_1'}"
 
     input:
     tuple val(meta), path(bams), path(indexes)
     tuple val(meta2), path(fai)
 
     output:
-    tuple val(meta), path("${prefix}/*")          , emit: output
-    tuple val(meta), path("${prefix}/*ped")       , emit: ped , optional: true
-    tuple val(meta), path("${prefix}/*bed.gz")    , emit: bed , optional: true
-    tuple val(meta), path("${prefix}/*bed.gz.tbi"), emit: bed_index , optional: true
-    tuple val(meta), path("${prefix}/*roc")       , emit: roc , optional: true
-    tuple val(meta), path("${prefix}/*html")      , emit: html, optional: true
-    tuple val(meta), path("${prefix}/*png")       , emit: png , optional: true
-    path "versions.yml"                           , emit: versions
+    tuple val(meta), path("${prefix}/*"),           emit: output
+    tuple val(meta), path("${prefix}/*ped"),        emit: ped,       optional: true
+    tuple val(meta), path("${prefix}/*bed.gz"),     emit: bed,       optional: true
+    tuple val(meta), path("${prefix}/*bed.gz.tbi"), emit: bed_index, optional: true
+    tuple val(meta), path("${prefix}/*roc"),        emit: roc,       optional: true
+    tuple val(meta), path("${prefix}/*html"),       emit: html,      optional: true
+    tuple val(meta), path("${prefix}/*png"),        emit: png,       optional: true
+    tuple val("${task.process}"), val('goleft'), eval("goleft --version |& sed '1!d;s/^.*goleft Version: //'"), topic: versions, emit: versions_goleft
+    tuple val("${task.process}"), val('tabix'), eval("tabix -h |& sed -n 's/^.*Version: //p'"), topic: versions, emit: versions_tabix
 
     when:
     task.ext.when == null || task.ext.when
@@ -28,38 +29,26 @@ process GOLEFT_INDEXCOV {
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     // indexcov uses BAM files or CRAI
-    def input_files = bams.findAll{it.name.endsWith(".bam")} + indexes.findAll{it.name.endsWith(".crai")}
-    def extranormalize = input_files.any{it.name.endsWith(".crai")} ? " --extranormalize " : ""
+    def input_files = bams.findAll {bam_file -> bam_file.name.endsWith(".bam") } + indexes.findAll {index_file -> index_file.name.endsWith(".crai") }
+    def extranormalize = input_files.any {input_file -> input_file.name.endsWith(".crai") } ? " --extranormalize " : ""
     """
     goleft indexcov \\
         --fai ${fai}  \\
         --directory ${prefix} \\
         ${extranormalize} \\
-        $args \\
+        ${args} \\
         ${input_files.join(" ")}
 
     if [ -f "${prefix}/${prefix}-indexcov.bed.gz" ] ; then
-        tabix -p bed "${prefix}/${prefix}-indexcov.bed.gz"
+        tabix -p bed ${prefix}/${prefix}-indexcov.bed.gz
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        goleft: \$(goleft --version 2>&1 | head -n 1 | sed 's/^.*goleft Version: //')
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
     """
+
     stub:
-    def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     """
-    mkdir "${prefix}"
-    echo "" | gzip > "${prefix}/${prefix}-indexcov.bed.gz"
-    touch "${prefix}/${prefix}-indexcov.bed.gz.tbi"
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        goleft: \$(goleft --version 2>&1 | head -n 1 | sed 's/^.*goleft Version: //')
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
+    mkdir ${prefix}
+    echo "" | gzip > ${prefix}/${prefix}-indexcov.bed.gz
+    touch ${prefix}/${prefix}-indexcov.bed.gz.tbi
     """
 }
diff --git a/modules/nf-core/goleft/indexcov/meta.yml b/modules/nf-core/goleft/indexcov/meta.yml
index 1619caf32d..b9165f9b6f 100644
--- a/modules/nf-core/goleft/indexcov/meta.yml
+++ b/modules/nf-core/goleft/indexcov/meta.yml
@@ -1,8 +1,7 @@
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json
 name: "goleft_indexcov"
-description: Quickly estimate coverage from a whole-genome bam or cram index. A bam
-  index has 16KB resolution so that's what this gives, but it provides what appears
-  to be a high-quality coverage estimate in seconds per genome.
+description: Quickly estimate coverage from a whole-genome bam or cram index. A
+  bam index has 16KB resolution so that's what this gives, but it provides what
+  appears to be a high-quality coverage estimate in seconds per genome.
 keywords:
   - coverage
   - cnv
@@ -16,7 +15,8 @@ tools:
       documentation: "https://github.com/brentp/goleft"
       tool_dev_url: "https://github.com/brentp/goleft"
       doi: "10.1093/gigascience/gix090"
-      licence: ["MIT"]
+      licence:
+        - "MIT"
       identifier: ""
 input:
   - - meta:
@@ -28,10 +28,12 @@ input:
         type: file
         description: Sorted BAM/CRAM/SAM files
         pattern: "*.{bam,cram,sam}"
+        ontologies: []
     - indexes:
         type: file
         description: BAI/CRAI files
         pattern: "*.{bai,crai}"
+        ontologies: []
   - - meta2:
         type: map
         description: |
@@ -41,9 +43,10 @@ input:
         type: file
         description: FASTA index
         pattern: "*.{fai}"
+        ontologies: []
 output:
-  - output:
-      - meta:
+  output:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -51,8 +54,9 @@ output:
       - ${prefix}/*:
           type: file
           description: Files generated by indexcov
-  - ped:
-      - meta:
+          ontologies: []
+  ped:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -61,8 +65,9 @@ output:
           type: file
           description: ped files
           pattern: "*ped"
-  - bed:
-      - meta:
+          ontologies: []
+  bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -71,8 +76,10 @@ output:
           type: file
           description: bed files
           pattern: "*bed.gz"
-  - bed_index:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  bed_index:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -81,8 +88,9 @@ output:
           type: file
           description: bed index files
           pattern: "*bed.gz.tbi"
-  - roc:
-      - meta:
+          ontologies: []
+  roc:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -91,8 +99,9 @@ output:
           type: file
           description: roc files
           pattern: "*roc"
-  - html:
-      - meta:
+          ontologies: []
+  html:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -101,8 +110,9 @@ output:
           type: file
           description: html files
           pattern: "*html"
-  - png:
-      - meta:
+          ontologies: []
+  png:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -111,11 +121,47 @@ output:
           type: file
           description: png files
           pattern: "*png"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_goleft:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - goleft:
+          type: string
+          description: The name of the tool
+      - "goleft --version |& sed '1!d;s/^.*goleft Version: //'":
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_tabix:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - tabix:
+          type: string
+          description: The name of the tool
+      - "tabix -h |& sed -n 's/^.*Version: //p'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - goleft:
+          type: string
+          description: The name of the tool
+      - "goleft --version |& sed '1!d;s/^.*goleft Version: //'":
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - tabix:
+          type: string
+          description: The name of the tool
+      - "tabix -h |& sed -n 's/^.*Version: //p'":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@lindenb"
 maintainers:
diff --git a/modules/nf-core/msisensor2/msi/main.nf b/modules/nf-core/msisensor2/msi/main.nf
index a15e2f66e2..51ecda3ca2 100644
--- a/modules/nf-core/msisensor2/msi/main.nf
+++ b/modules/nf-core/msisensor2/msi/main.nf
@@ -3,19 +3,19 @@ process MSISENSOR2_MSI {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 
-            'https://depot.galaxyproject.org/singularity/msisensor2:0.1--hd03093a_0' :
-            'biocontainers/msisensor2:0.1--hd03093a_0'}"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/msisensor2:0.1--hd03093a_0'
+        : 'quay.io/biocontainers/msisensor2:0.1--hd03093a_0'}"
 
     input:
     tuple val(meta), path(tumor_bam), path(tumor_bam_index)
     tuple val(meta2), path(models)
 
     output:
-    tuple val(meta), path("${prefix}"),          emit: msi
-    tuple val(meta), path("${prefix}_dis"),      emit: distribution
-    tuple val(meta), path("${prefix}_somatic"),  emit: somatic
-    path "versions.yml",                         emit: versions
+    tuple val(meta), path("${prefix}"), emit: msi
+    tuple val(meta), path("${prefix}_dis"), emit: distribution
+    tuple val(meta), path("${prefix}_somatic"), emit: somatic
+    tuple val("${task.process}"), val('msisensor2'), eval("msisensor2 2> >(grep Version) | sed 's/Version: v//g'"), topic: versions, emit: versions_msisensor2
 
     when:
     task.ext.when == null || task.ext.when
@@ -30,11 +30,6 @@ process MSISENSOR2_MSI {
         -M ${models} \\
         -t ${tumor_bam} \\
         -o ${prefix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor2: \$(echo \$(msisensor2 2> >(grep Version) | sed 's/Version: v//g'))
-    END_VERSIONS
     """
 
     stub:
@@ -43,10 +38,5 @@ process MSISENSOR2_MSI {
     touch ${prefix}
     touch ${prefix}_dis
     touch ${prefix}_somatic
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor2: \$(echo \$(msisensor2 2> >(grep Version) | sed 's/Version: v//g'))
-    END_VERSIONS
     """
-}
\ No newline at end of file
+}
diff --git a/modules/nf-core/msisensor2/msi/meta.yml b/modules/nf-core/msisensor2/msi/meta.yml
index 9cece656ac..af03315190 100644
--- a/modules/nf-core/msisensor2/msi/meta.yml
+++ b/modules/nf-core/msisensor2/msi/meta.yml
@@ -16,7 +16,8 @@ tools:
       homepage: "https://github.com/niu-lab/msisensor2"
       documentation: "https://github.com/niu-lab/msisensor2/blob/master/README.md"
       tool_dev_url: "https://github.com/niu-lab/msisensor2"
-      license: ["GPL-3.0"]
+      license:
+        - "GPL-3.0"
       identifier: ""
 input:
   - - meta:
@@ -76,13 +77,27 @@ output:
           type: file
           description: Somatic MSI regions detected.
           ontologies: []
+  versions_msisensor2:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor2:
+          type: string
+          description: The name of the tool
+      - "msisensor2 2> >(grep Version) | sed 's/Version: v//g'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor2:
+          type: string
+          description: The name of the tool
+      - "msisensor2 2> >(grep Version) | sed 's/Version: v//g'":
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
 maintainers:
diff --git a/modules/nf-core/msisensorpro/msisomatic/main.nf b/modules/nf-core/msisensorpro/msisomatic/main.nf
index 41e8a04cfb..bee22f679c 100644
--- a/modules/nf-core/msisensorpro/msisomatic/main.nf
+++ b/modules/nf-core/msisensorpro/msisomatic/main.nf
@@ -3,9 +3,9 @@ process MSISENSORPRO_MSISOMATIC {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
             'https://depot.galaxyproject.org/singularity/msisensor-pro%3A1.3.0--hfef96ef_0':
-            'biocontainers/msisensor-pro:1.3.0--hfef96ef_0' }"
+            'quay.io/biocontainers/msisensor-pro:1.3.0--hfef96ef_0' }"
 
     input:
     tuple val(meta), path(normal), path(normal_index), path(tumor), path(tumor_index), path(intervals)
@@ -17,7 +17,7 @@ process MSISENSORPRO_MSISOMATIC {
     tuple val(meta), path("${prefix}_dis")     , emit: output_dis
     tuple val(meta), path("${prefix}_germline"), emit: output_germline, optional: true
     tuple val(meta), path("${prefix}_somatic") , emit: output_somatic,  optional: true
-    path "versions.yml"                        , emit: versions
+    tuple val("${task.process}"), val('msisensor-pro'), eval("msisensor-pro --version 2>&1 | sed -nE 's/Version:\\s*v//p'") , emit: versions_msisensorpro, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -39,11 +39,6 @@ process MSISENSORPRO_MSISOMATIC {
         -b ${task.cpus} \\
         ${intervals_cmd} \\
         ${args}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p')
-    END_VERSIONS
     """
 
     stub:
@@ -54,10 +49,5 @@ process MSISENSORPRO_MSISOMATIC {
     touch ${prefix}_dis
     touch ${prefix}_germline
     touch ${prefix}_somatic
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/msisensorpro/msisomatic/meta.yml b/modules/nf-core/msisensorpro/msisomatic/meta.yml
index 1c05e25e3c..26f91b00ee 100644
--- a/modules/nf-core/msisensorpro/msisomatic/meta.yml
+++ b/modules/nf-core/msisensorpro/msisomatic/meta.yml
@@ -1,7 +1,8 @@
 name: msisensorpro_msisomatic
-description: MSIsensor-pro evaluates Microsatellite Instability (MSI) for cancer patients
-  with next generation sequencing data. It accepts the whole genome sequencing, whole
-  exome sequencing and target region (panel) sequencing data as input
+description: MSIsensor-pro evaluates Microsatellite Instability (MSI) for cancer
+  patients with next generation sequencing data. It accepts the whole genome
+  sequencing, whole exome sequencing and target region (panel) sequencing data
+  as input
 keywords:
   - micro-satellite-scan
   - msisensor-pro
@@ -9,13 +10,14 @@ keywords:
   - somatic
 tools:
   - msisensorpro:
-      description: Microsatellite Instability (MSI) detection using high-throughput
-        sequencing data.
+      description: Microsatellite Instability (MSI) detection using
+        high-throughput sequencing data.
       homepage: https://github.com/xjtu-omics/msisensor-pro
       documentation: https://github.com/xjtu-omics/msisensor-pro/wiki
       tool_dev_url: https://github.com/xjtu-omics/msisensor-pro
       doi: "10.1016/j.gpb.2020.02.001"
-      licence: ["Custom Licence"]
+      licence:
+        - "Custom Licence"
       identifier: ""
 input:
   - - meta:
@@ -72,8 +74,8 @@ output:
             e.g. [ id:'test', single_end:false ]
       - ${prefix}:
           type: file
-          description: File containing final report with all detected microsatellites,
-            unstable somatic microsatellites, msi score
+          description: File containing final report with all detected
+            microsatellites, unstable somatic microsatellites, msi score
           ontologies: []
   output_dis:
     - - meta:
@@ -105,13 +107,27 @@ output:
           type: file
           description: File containing somatic results
           ontologies: []
+  versions_msisensorpro:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor-pro:
+          type: string
+          description: The name of the tool
+      - msisensor-pro --version 2>&1 | sed -nE 's/Version:\s*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor-pro:
+          type: string
+          description: The name of the tool
+      - msisensor-pro --version 2>&1 | sed -nE 's/Version:\s*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/modules/nf-core/msisensorpro/scan/main.nf b/modules/nf-core/msisensorpro/scan/main.nf
index 7ef4ae172c..8d2859d7ca 100644
--- a/modules/nf-core/msisensorpro/scan/main.nf
+++ b/modules/nf-core/msisensorpro/scan/main.nf
@@ -3,16 +3,16 @@ process MSISENSORPRO_SCAN {
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
             'https://depot.galaxyproject.org/singularity/msisensor-pro%3A1.3.0--hfef96ef_0':
-            'biocontainers/msisensor-pro:1.3.0--hfef96ef_0' }"
+            'quay.io/biocontainers/msisensor-pro:1.3.0--hfef96ef_0' }"
 
     input:
     tuple val(meta), path(fasta)
 
     output:
     tuple val(meta), path("*.list"), emit: list
-    path "versions.yml"            , emit: versions
+    tuple val("${task.process}"), val('msisensor-pro'), eval("msisensor-pro --version 2>&1 | sed -nE 's/Version:\\s*v//p'") , emit: versions_msisensorpro, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,21 +26,11 @@ process MSISENSORPRO_SCAN {
         -d $fasta \\
         -o ${prefix}.msisensor_scan.list \\
         $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p')
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.msisensor_scan.list
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        msisensor-pro: \$(msisensor-pro 2>&1 | sed -nE 's/Version:\\sv([0-9]\\.[0-9])/\\1/ p')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/msisensorpro/scan/meta.yml b/modules/nf-core/msisensorpro/scan/meta.yml
index 2380922c21..c255d53297 100644
--- a/modules/nf-core/msisensorpro/scan/meta.yml
+++ b/modules/nf-core/msisensorpro/scan/meta.yml
@@ -1,20 +1,22 @@
 name: msisensorpro_scan
-description: MSIsensor-pro evaluates Microsatellite Instability (MSI) for cancer patients
-  with next generation sequencing data. It accepts the whole genome sequencing, whole
-  exome sequencing and target region (panel) sequencing data as input
+description: MSIsensor-pro evaluates Microsatellite Instability (MSI) for cancer
+  patients with next generation sequencing data. It accepts the whole genome
+  sequencing, whole exome sequencing and target region (panel) sequencing data
+  as input
 keywords:
   - micro-satellite-scan
   - msisensor-pro
   - scan
 tools:
   - msisensorpro:
-      description: Microsatellite Instability (MSI) detection using high-throughput
-        sequencing data.
+      description: Microsatellite Instability (MSI) detection using
+        high-throughput sequencing data.
       homepage: https://github.com/xjtu-omics/msisensor-pro
       documentation: https://github.com/xjtu-omics/msisensor-pro/wiki
       tool_dev_url: https://github.com/xjtu-omics/msisensor-pro
       doi: "10.1016/j.gpb.2020.02.001"
-      licence: ["Custom Licence"]
+      licence:
+        - "Custom Licence"
       identifier: ""
 input:
   - - meta:
@@ -39,13 +41,27 @@ output:
           description: File containing microsatellite list
           pattern: "*.{list}"
           ontologies: []
+  versions_msisensorpro:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor-pro:
+          type: string
+          description: The name of the tool
+      - msisensor-pro --version 2>&1 | sed -nE 's/Version:\s*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
   versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - msisensor-pro:
+          type: string
+          description: The name of the tool
+      - msisensor-pro --version 2>&1 | sed -nE 's/Version:\s*v//p':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@FriederikeHanssen"
 maintainers:
diff --git a/subworkflows/local/bam_markduplicates/main.nf b/subworkflows/local/bam_markduplicates/main.nf
index 2e770e732c..a5ca322259 100644
--- a/subworkflows/local/bam_markduplicates/main.nf
+++ b/subworkflows/local/bam_markduplicates/main.nf
@@ -15,7 +15,6 @@ workflow BAM_MARKDUPLICATES {
     intervals_bed_combined // channel: [optional]  [ intervals_bed ]
 
     main:
-    versions = channel.empty()
     reports  = channel.empty()
 
     // RUN MARKUPDUPLICATES
@@ -36,12 +35,7 @@ workflow BAM_MARKDUPLICATES {
     reports = reports.mix(GATK4_MARKDUPLICATES.out.metrics)
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
-    // Gather versions of all tools used
-    versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
-
     emit:
     alignment   // channel: [ meta, file, index ] — BAM or CRAM
     reports
-
-    versions    // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_markduplicates_spark/main.nf b/subworkflows/local/bam_markduplicates_spark/main.nf
index 0c9172b706..a2883f3884 100644
--- a/subworkflows/local/bam_markduplicates_spark/main.nf
+++ b/subworkflows/local/bam_markduplicates_spark/main.nf
@@ -18,7 +18,6 @@ workflow BAM_MARKDUPLICATES_SPARK {
     intervals_bed_combined        // channel: [optional]  intervals_bed
 
     main:
-    versions = channel.empty()
     reports = channel.empty()
 
     // RUN MARKUPDUPLICATES SPARK
@@ -41,12 +40,8 @@ workflow BAM_MARKDUPLICATES_SPARK {
     reports = reports.mix(GATK4_ESTIMATELIBRARYCOMPLEXITY.out.metrics)
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
-    // Gather versions of all tools used
-    versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
 
     emit:
     alignment   // channel: [ meta, file, index ] — BAM or CRAM
     reports
-
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_sentieon_dedup/main.nf b/subworkflows/local/bam_sentieon_dedup/main.nf
index 151febcc39..1abe715464 100644
--- a/subworkflows/local/bam_sentieon_dedup/main.nf
+++ b/subworkflows/local/bam_sentieon_dedup/main.nf
@@ -13,7 +13,6 @@ workflow BAM_SENTIEON_DEDUP {
     intervals_bed_combined // channel: [optional]  [ intervals_bed ]
 
     main:
-    versions = channel.empty()
     reports  = channel.empty()
 
     bam = bam.map{ meta, bam_ -> [ meta - meta.subMap('data_type'), bam_ ] }
@@ -38,12 +37,7 @@ workflow BAM_SENTIEON_DEDUP {
     reports = reports.mix(SENTIEON_DEDUP.out.score)
     reports = reports.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.reports)
 
-    // Gather versions of all tools used
-    versions = versions.mix(CRAM_QC_MOSDEPTH_SAMTOOLS.out.versions)
-
     emit:
     alignment   // channel: [ meta, file, index ] — BAM or CRAM
     reports
-
-    versions    // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_variant_calling_indexcov/main.nf b/subworkflows/local/bam_variant_calling_indexcov/main.nf
index 4255de91cc..59c261d10c 100644
--- a/subworkflows/local/bam_variant_calling_indexcov/main.nf
+++ b/subworkflows/local/bam_variant_calling_indexcov/main.nf
@@ -34,9 +34,6 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
         fasta_fai
         )
 
-    versions = versions.mix(goleft_ch.versions)
-
-
     emit:
 
     out_indexcov = goleft_ch.output
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index 4ca65663ad..2c43feee40 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -83,7 +83,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             rt_file,
         )
 
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_ASCAT.out.versions)
     }
 
     // CONTROLFREEC
@@ -201,7 +200,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
     if (tools && tools.split(',').contains('msisensorpro')) {
         MSISENSORPRO_MSISOMATIC(cram.combine(intervals_bed_combined), fasta, msisensorpro_scan)
 
-        versions = versions.mix(MSISENSORPRO_MSISOMATIC.out.versions)
         out_msisensorpro = out_msisensorpro.mix(MSISENSORPRO_MSISOMATIC.out.output_report)
     }
 
diff --git a/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf b/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
index f771726eed..015533444b 100644
--- a/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_ascat/main.nf
@@ -19,12 +19,5 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ASCAT {
 
     main:
 
-    ch_versions = channel.empty()
-
     ASCAT(cram_pair, allele_files, loci_files, intervals_bed, fasta, gc_file, rt_file)
-
-    ch_versions = ch_versions.mix(ASCAT.out.versions)
-
-    emit:
-    versions = ch_versions
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index 9b83123f0f..b72ffb0fea 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -126,7 +126,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
 
         MSISENSOR2_MSI(bam, msisensor2_models)
 
-        versions = versions.mix(MSISENSOR2_MSI.out.versions)
         out_msisensor2 = out_msisensor2.mix(MSISENSOR2_MSI.out.distribution)
         out_msisensor2 = out_msisensor2.mix(MSISENSOR2_MSI.out.somatic)
     }
diff --git a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
index d5a1371cb3..db88c83141 100644
--- a/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
+++ b/subworkflows/local/cram_qc_mosdepth_samtools/main.nf
@@ -15,7 +15,6 @@ workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
     intervals
 
     main:
-    versions = channel.empty()
     reports = channel.empty()
 
     // Reports run on cram
@@ -30,5 +29,4 @@ workflow CRAM_QC_MOSDEPTH_SAMTOOLS {
 
     emit:
     reports
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/cram_sampleqc/main.nf b/subworkflows/local/cram_sampleqc/main.nf
index 4ac78c2af8..1a36e6a89e 100644
--- a/subworkflows/local/cram_sampleqc/main.nf
+++ b/subworkflows/local/cram_sampleqc/main.nf
@@ -10,7 +10,6 @@ workflow CRAM_SAMPLEQC {
     intervals_for_preprocessing // channel:
 
     main:
-    versions = channel.empty()
     reports = channel.empty()
 
     if (!skip_baserecalibration) {
@@ -24,9 +23,6 @@ workflow CRAM_SAMPLEQC {
 
         // Gather QC reports
         reports = CRAM_QC_RECAL.out.reports.collect { _meta, report -> report }
-
-        // Gather used softwares versions
-        versions = versions.mix(CRAM_QC_RECAL.out.versions)
     }
 
     BAM_NGSCHECKMATE(cram.map { meta, cram_, _crai -> [meta, cram_] }, ngscheckmate_bed.map { bed -> [[id: "ngscheckmate"], bed] }, fasta_fai)
@@ -38,5 +34,4 @@ workflow CRAM_SAMPLEQC {
     vcf         = BAM_NGSCHECKMATE.out.vcf // channel: [ meta, vcf ]
     pdf         = BAM_NGSCHECKMATE.out.pdf // channel: [ meta, pdf ]
     reports
-    versions    // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index 6fdb2613ee..044dd0173e 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -63,7 +63,6 @@ workflow FASTQ_PREPROCESS_GATK {
 
     // To gather all QC reports for MultiQC
     reports          = channel.empty()
-    versions         = channel.empty()
 
     // PREPROCESSING
 
@@ -95,8 +94,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 interleave_input)
 
             reads_for_fastp = CONVERT_FASTQ_UMI.out.reads
-
-            // Gather used softwares versions
         } else {
             reads_for_fastp = input_fastq
         }
@@ -236,8 +233,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Create CSV to restart from this step
             if (params.save_output_as_bam) CHANNEL_ALIGN_CREATE_CSV(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, params.outdir, params.save_output_as_bam)
             else CHANNEL_ALIGN_CREATE_CSV(BAM_TO_CRAM_MAPPING.out.cram.join(BAM_TO_CRAM_MAPPING.out.crai, failOnDuplicate: true, failOnMismatch: true), params.outdir, params.save_output_as_bam)
-
-            // Gather used softwares versions
         }
 
     }
@@ -283,8 +278,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Gather QC reports
             reports = reports.mix(CRAM_QC_NO_MD.out.reports.collect{ _meta, report -> [ report ] })
 
-            // Gather used softwares versions
-            versions = versions.mix(CRAM_QC_NO_MD.out.versions)
         } else if (params.use_gatk_spark && params.use_gatk_spark.contains('markduplicates')) {
             BAM_MARKDUPLICATES_SPARK(
                 cram_for_markduplicates,
@@ -297,8 +290,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Gather QC reports
             reports = reports.mix(BAM_MARKDUPLICATES_SPARK.out.reports.collect{ _meta, report -> [ report ] })
 
-            // Gather used softwares versions
-            versions = versions.mix(BAM_MARKDUPLICATES_SPARK.out.versions)
         } else if (params.tools && params.tools.split(',').contains('sentieon_dedup')) {
             crai_for_markduplicates = params.step == 'mapping'
                 ? bai_mapped
@@ -315,8 +306,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Gather QC reports
             reports = reports.mix(BAM_SENTIEON_DEDUP.out.reports.collect{ _meta, report -> [ report ] })
 
-            // Gather used softwares versions
-            versions = versions.mix(BAM_SENTIEON_DEDUP.out.versions)
         } else {
 
             BAM_MARKDUPLICATES(
@@ -330,8 +319,6 @@ workflow FASTQ_PREPROCESS_GATK {
             // Gather QC reports
             reports = reports.mix(BAM_MARKDUPLICATES.out.reports.collect{ _meta, report -> [ report ] })
 
-            // Gather used softwares versions
-            versions = versions.mix(BAM_MARKDUPLICATES.out.versions)
         }
 
         // ch_md_cram_for_restart contains either:
@@ -389,8 +376,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 known_sites_indels_tbi)
 
                 ch_table_bqsr_spark = BAM_BASERECALIBRATOR_SPARK.out.table_bqsr
-
-                // Gather used softwares versions
             } else {
 
             BAM_BASERECALIBRATOR(
@@ -403,8 +388,6 @@ workflow FASTQ_PREPROCESS_GATK {
                 known_sites_indels_tbi)
 
                 ch_table_bqsr_no_spark = BAM_BASERECALIBRATOR.out.table_bqsr
-
-                // Gather used softwares versions
             }
 
             // ch_table_bqsr contains either:
@@ -447,9 +430,6 @@ workflow FASTQ_PREPROCESS_GATK {
                     intervals_and_num_intervals)
 
                 cram_variant_calling_spark = BAM_APPLYBQSR_SPARK.out.alignment
-
-                // Gather used softwares versions
-
             } else {
 
                 BAM_APPLYBQSR(
@@ -460,8 +440,6 @@ workflow FASTQ_PREPROCESS_GATK {
                     intervals_and_num_intervals)
 
                 cram_variant_calling_no_spark = BAM_APPLYBQSR.out.alignment
-
-                // Gather used softwares versions
             }
 
             cram_variant_calling = channel.empty().mix(
@@ -486,6 +464,5 @@ workflow FASTQ_PREPROCESS_GATK {
     emit:
     cram_variant_calling
     reports
-    versions
 
 }
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index c52b73d911..9856373a6c 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -219,7 +219,6 @@ workflow PREPARE_GENOME {
     else if (tools.split(',').contains('msisensorpro')) {
         MSISENSORPRO_SCAN(fasta)
         msisensorpro_scan = MSISENSORPRO_SCAN.out.list.map { _meta, list -> [list] }.collect()
-        versions = versions.mix(MSISENSORPRO_SCAN.out.versions)
     }
     else {
         msisensorpro_scan = channel.value([])
diff --git a/tests/variant_calling_ascat.nf.test.snap b/tests/variant_calling_ascat.nf.test.snap
index d2078a5658..ac5d21fc53 100644
--- a/tests/variant_calling_ascat.nf.test.snap
+++ b/tests/variant_calling_ascat.nf.test.snap
@@ -195,7 +195,7 @@
             {
                 "ASCAT": {
                     "alleleCounter": "4.3.0",
-                    "ascat": "3.2.0"
+                    "bioconductor-ascat": "3.2.0"
                 },
                 "MOSDEPTH": {
                     "gzip": "1.14",
@@ -347,7 +347,7 @@
             {
                 "ASCAT": {
                     "alleleCounter": "4.3.0",
-                    "ascat": "3.2.0"
+                    "bioconductor-ascat": "3.2.0"
                 },
                 "MOSDEPTH": {
                     "gzip": "1.14",
diff --git a/tests/variant_calling_msisensor2.nf.test.snap b/tests/variant_calling_msisensor2.nf.test.snap
index db76dbf777..570a51c0f9 100644
--- a/tests/variant_calling_msisensor2.nf.test.snap
+++ b/tests/variant_calling_msisensor2.nf.test.snap
@@ -171,7 +171,7 @@
                     "mosdepth": "0.3.14"
                 },
                 "MSISENSOR2_MSI": {
-                    "msisensor2": 0.1
+                    "msisensor2": "0.1"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": "1.24"
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index da01b6bf97..5df7d648d1 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -246,9 +246,8 @@ workflow SAREK {
             cram_variant_calling = channel.empty()
             cram_variant_calling = cram_variant_calling.mix(FASTQ_PREPROCESS_GATK.out.cram_variant_calling)
 
-            // Gather used softwares versions
+            // Gather QC reports
             reports = reports.mix(FASTQ_PREPROCESS_GATK.out.reports)
-            versions = versions.mix(FASTQ_PREPROCESS_GATK.out.versions)
         }
     }
 
@@ -272,7 +271,6 @@ workflow SAREK {
     )
 
     reports = reports.mix(CRAM_SAMPLEQC.out.reports)
-    versions = versions.mix(CRAM_SAMPLEQC.out.versions)
 
     if (tools) {
 

From aa42b5d538aa250f9e1bc32b5cee2333eefc65d9 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Wed, 29 Jul 2026 10:18:21 +0200
Subject: [PATCH 19/27] chore(modules): migrate cnvkit/tabix modules to
 versions topic channel (#2243)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates `cnvkit/*` modules to the `versions` topic channel and updates
tabix. Base: `dev`.

### Changes
- Updates `cnvkit/{antitarget,batch,call,export,genemetrics,reference}`
to their topic-channel versions.
- Removes the corresponding `.out.versions` wiring.
- Rewires `cnvkit/batch` for its new input signature: `fasta`+`fai`
combined into one tuple; tumor/normal now carry index slots (`[]`,
unused by the script — it indexes internally).
- Tool bump: cnvkit 0.9.11 → 0.9.12.
- Removes dead `versions` channel plumbing left behind by modules that
had already moved to the topic channel via unrelated upstream syncs
(`sentieon/*`, `bcftools/sort`, `bcftools/isec`, `bcftools/concat`,
`ensemblvep/vep`, `snpsift/annmem`, `consensus_from_sites`), across
`bam_joint_calling_germline_sentieon`,
`bam_variant_calling_sentieon_dnascope`/`_haplotyper`,
`bam_variant_calling_somatic_tnscope`/`_tumor_only_tnscope`,
`vcf_consensus`, `vcf_annotate_all`, and
`bam_variant_calling_tumor_only_all`.
- Replaces the upstream-deprecated `tabix/tabix` and `tabix/bgziptabix`
modules (both now `assert false` on any future module update) with
`htslib/bgziptabix` across all 15 real call sites plus the vendored
`vcf_annotate_snpeff` subworkflow.

Test snapshots need regenerating in CI.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   7 +-
 conf/base.config                              |   2 +-
 conf/modules/annotate.config                  |   6 +-
 conf/modules/freebayes.config                 |   2 +
 conf/modules/post_variant_calling.config      |   2 +-
 conf/modules/prepare_genome.config            |   6 +
 conf/modules/varlociraptor.config             |   1 +
 main.nf                                       |   2 -
 modules.json                                  |  29 ++--
 .../nf-core/cnvkit/antitarget/environment.yml |   2 +-
 modules/nf-core/cnvkit/antitarget/main.nf     |  23 +--
 modules/nf-core/cnvkit/antitarget/meta.yml    |  32 ++++-
 modules/nf-core/cnvkit/batch/environment.yml  |   6 +-
 modules/nf-core/cnvkit/batch/main.nf          | 106 +++++++-------
 modules/nf-core/cnvkit/batch/meta.yml         | 131 ++++++++++++++----
 modules/nf-core/cnvkit/call/environment.yml   |   2 +-
 modules/nf-core/cnvkit/call/main.nf           |  30 ++--
 modules/nf-core/cnvkit/call/meta.yml          |  40 ++++--
 modules/nf-core/cnvkit/export/environment.yml |   2 +-
 modules/nf-core/cnvkit/export/main.nf         |  24 +---
 modules/nf-core/cnvkit/export/meta.yml        |  39 ++++--
 .../cnvkit/genemetrics/environment.yml        |   2 +-
 modules/nf-core/cnvkit/genemetrics/main.nf    |  27 ++--
 modules/nf-core/cnvkit/genemetrics/meta.yml   |  47 ++++---
 .../nf-core/cnvkit/reference/environment.yml  |   2 +-
 modules/nf-core/cnvkit/reference/main.nf      |  30 ++--
 modules/nf-core/cnvkit/reference/meta.yml     |  69 +++++----
 .../bgziptabix/environment.yml                |   4 +-
 modules/nf-core/htslib/bgziptabix/main.nf     |  88 ++++++++++++
 modules/nf-core/htslib/bgziptabix/meta.yml    | 125 +++++++++++++++++
 modules/nf-core/tabix/bgziptabix/main.nf      |  40 ------
 modules/nf-core/tabix/bgziptabix/meta.yml     |  93 -------------
 modules/nf-core/tabix/tabix/environment.yml   |   8 --
 modules/nf-core/tabix/tabix/main.nf           |  45 ------
 modules/nf-core/tabix/tabix/meta.yml          |  63 ---------
 .../main.nf                                   |   4 -
 .../local/bam_variant_calling_cnvkit/main.nf  |  13 +-
 .../bam_variant_calling_freebayes/main.nf     |  18 +--
 .../bam_variant_calling_germline_all/main.nf  |   8 --
 .../main.nf                                   |   3 -
 .../main.nf                                   |   3 -
 .../bam_variant_calling_single_tiddit/main.nf |   8 +-
 .../bam_variant_calling_somatic_all/main.nf   |   4 -
 .../main.nf                                   |   3 -
 .../main.nf                                   |   8 --
 .../main.nf                                   |   3 -
 .../local/post_variantcalling/main.nf         |   3 -
 subworkflows/local/prepare_genome/main.nf     |  44 +++---
 .../prepare_genome/tests/bbsplit.nf.test      |  12 +-
 subworkflows/local/prepare_intervals/main.nf  |  14 +-
 .../local/prepare_reference_cnvkit/main.nf    |   6 -
 subworkflows/local/vcf_annotate_all/main.nf   |   2 -
 .../local/vcf_concatenate_germline/main.nf    |   7 +-
 subworkflows/local/vcf_consensus/main.nf      |   4 -
 subworkflows/local/vcf_normalization/main.nf  |   6 +-
 .../local/vcf_varlociraptor_somatic/main.nf   |  16 +--
 .../nf-core/vcf_annotate_snpeff/main.nf       |  18 ++-
 .../nf-core/vcf_annotate_snpeff/meta.yml      |   2 +-
 tests/aligner-bwa-mem.nf.test.snap            |  48 +++----
 tests/aligner-bwa-mem2.nf.test.snap           |  44 +++---
 tests/aligner-dragmap.nf.test.snap            |  44 +++---
 tests/aligner-parabricks.nf.test.snap         |  40 +++---
 tests/alignment_from_everything.nf.test.snap  |   8 +-
 tests/alignment_to_fastq.nf.test.snap         |   8 +-
 tests/annotation_merge.nf.test.snap           |  36 ++---
 tests/bbsplit.nf.test.snap                    |  26 ++--
 tests/default.nf.test.snap                    |  16 +--
 tests/fastp.nf.test.snap                      |  24 ++--
 tests/intervals.nf.test.snap                  |  58 ++++----
 ...joint_calling_haplotypecaller.nf.test.snap |  16 +--
 tests/joint_calling_mutect2.nf.test.snap      |  18 +--
 tests/lane_integer.nf.test.snap               |  16 +--
 tests/multi_lane.nf.test.snap                 |  26 ++--
 tests/postprocess_concatenation.nf.test.snap  |  18 +--
 ...s_concatenation_normalization.nf.test.snap |  36 ++---
 tests/postprocess_consensus.nf.test.snap      |  36 ++---
 tests/postprocess_filtering.nf.test.snap      |  14 +-
 tests/postprocess_normalization.nf.test.snap  |  18 +--
 tests/postprocess_varlociraptor.nf.test.snap  |  35 ++---
 tests/qc_ngscheckmate.nf.test.snap            |  16 +--
 tests/save_mapped.nf.test.snap                |  16 +--
 tests/save_output_as_bam.nf.test.snap         |  55 ++++----
 tests/sentieon.nf.test.snap                   |   8 +-
 tests/sentieon_aligner_bwamem.nf.test.snap    |  48 +++----
 tests/sentieon_dedup.nf.test.snap             |  48 +++----
 tests/spark.nf.test.snap                      |  24 ++--
 tests/start_from_markduplicates.nf.test.snap  |  34 ++---
 ...art_from_preparerecalibration.nf.test.snap |  50 +++----
 tests/start_from_recalibration.nf.test.snap   |  48 +++----
 tests/tumor-normal-pair.nf.test.snap          |   8 +-
 tests/umi_fastp.nf.test.snap                  |   8 +-
 tests/umi_fgbio.nf.test.snap                  |   8 +-
 tests/umi_in_read_names.nf.test.snap          |  18 +--
 tests/variant_calling_all.nf.test.snap        |  95 +++++++------
 tests/variant_calling_ascat.nf.test.snap      |  16 +--
 tests/variant_calling_cnvkit.nf.test.snap     | 113 +++++++--------
 .../variant_calling_controlfreec.nf.test.snap |  24 ++--
 .../variant_calling_deepvariant.nf.test.snap  |  26 ++--
 tests/variant_calling_freebayes.nf.test.snap  |  67 +++++----
 ...riant_calling_haplotypecaller.nf.test.snap |  24 ++--
 tests/variant_calling_lofreq.nf.test.snap     |  12 +-
 tests/variant_calling_manta.nf.test.snap      |  50 +++----
 tests/variant_calling_mpileup.nf.test.snap    |  24 ++--
 tests/variant_calling_msisensor2.nf.test.snap |  26 ++--
 .../variant_calling_msisensorpro.nf.test.snap |  26 ++--
 tests/variant_calling_muse.nf.test.snap       |  16 +--
 tests/variant_calling_mutect2.nf.test.snap    |  28 ++--
 ...ant_calling_sentieon_dnascope.nf.test.snap |  30 ++--
 ...ling_sentieon_haplotypecaller.nf.test.snap |  34 ++---
 ...iant_calling_sentieon_tnscope.nf.test.snap |  60 ++++----
 tests/variant_calling_strelka.nf.test.snap    |  34 ++---
 tests/variant_calling_strelka_bp.nf.test.snap |  12 +-
 tests/variant_calling_tiddit.nf.test.snap     |  36 ++---
 workflows/sarek.nf                            |   4 -
 114 files changed, 1585 insertions(+), 1492 deletions(-)
 rename modules/nf-core/{tabix => htslib}/bgziptabix/environment.yml (77%)
 create mode 100644 modules/nf-core/htslib/bgziptabix/main.nf
 create mode 100644 modules/nf-core/htslib/bgziptabix/meta.yml
 delete mode 100644 modules/nf-core/tabix/bgziptabix/main.nf
 delete mode 100644 modules/nf-core/tabix/bgziptabix/meta.yml
 delete mode 100644 modules/nf-core/tabix/tabix/environment.yml
 delete mode 100644 modules/nf-core/tabix/tabix/main.nf
 delete mode 100644 modules/nf-core/tabix/tabix/meta.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index e5d006f4e3..b815a4a08f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,7 +22,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2239](https://github.com/nf-core/sarek/pull/2239) - Migrate alignment/UMI/utility modules (`bwa`, `bwamem2`, `dragmap`, `fgbio`, `fastp`, `cat`, `gawk`, `gunzip`, `untar`, `unzip`, `spring`) to the versions topic channel (fastp 0.24.0 → 1.1.0)
 - [#2240](https://github.com/nf-core/sarek/pull/2240) - Migrate variant-calling modules (`freebayes`, `strelka`, `manta`, `tiddit`, `lofreq`, `svdb`, `vcflib`, `vcftools`) to the versions topic channel
 - [#2241](https://github.com/nf-core/sarek/pull/2241) - Migrate `samtools/*` and `mosdepth` modules to the versions topic channel
-- [#2242](https://github.com/nf-core/sarek/pull/2242) - Migrate QC/coverage modules (`fastqc`, `ngscheckmate`, `goleft`, `msisensor2`, `msisensorpro`, `ascat`) to the versions topic channel
+- [#2242](https://github.com/nf-core/sarek/pull/2242) - Migrate QC/coverage modules (`ascat`, `goleft`, `msisensor2`, `msisensorpro`) to the versions topic channel
+- [#2243](https://github.com/nf-core/sarek/pull/2243) - Migrate `cnvkit/*` modules to the versions topic channel, and replace the deprecated `tabix/tabix`/`tabix/bgziptabix` modules with `htslib/bgziptabix`
 
 ### Fixed
 
@@ -37,7 +38,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | Dependency    | Old version | New version |
 | ------------- | ----------- | ----------- |
 | bcftools      | 1.21        | 1.23.1      |
-| htslib        | 1.21        | 1.23.1      |
+| htslib        | 1.21        | 1.24        |
 | varlociraptor | 8.9.3       | 8.9.5       |
 | ensembl-vep   | 115.2       | 116.0       |
 | gatk4         | 4.6.1.0     | 4.6.2.0     |
@@ -53,6 +54,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | vcftools      | 0.1.16      | 0.1.17      |
 | samtools      | 1.21        | 1.24        |
 | mosdepth      | 0.3.10      | 0.3.14      |
+| cnvkit        | 0.9.11      | 0.9.12      |
+| xz            | -           | 5.8.3       |
 
 ### Dependencies - plugins
 
diff --git a/conf/base.config b/conf/base.config
index 4e184eca9c..63d7104837 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -58,7 +58,7 @@ process {
     withLabel:process_high_memory {
         memory = { 200.GB * task.attempt }
     }
-    withName: 'UNZIP.*|UNTAR.*|TABIX.*|BUILD_INTERVALS|CREATE_INTERVALS_BED|VCFTOOLS|BCFTOOLS.*|SAMTOOLS_INDEX' {
+    withName: 'UNZIP.*|UNTAR.*|TABIX.*|HTSLIB_BGZIPTABIX|BUILD_INTERVALS|CREATE_INTERVALS_BED|VCFTOOLS|BCFTOOLS.*|SAMTOOLS_INDEX' {
         cpus   = { 1    * task.attempt }
         memory = { 1.GB * task.attempt }
     }
diff --git a/conf/modules/annotate.config b/conf/modules/annotate.config
index 35a160b4d2..065baabb6e 100644
--- a/conf/modules/annotate.config
+++ b/conf/modules/annotate.config
@@ -109,8 +109,8 @@ process {
         }
 
     // ALL ANNOTATION TOOLS
-        withName: 'NFCORE_SAREK:SAREK:VCF_ANNOTATE_ALL:.*:(TABIX_BGZIPTABIX|TABIX_TABIX)' {
-            ext.prefix = { input.name - '.vcf' }
+        withName: 'NFCORE_SAREK:SAREK:VCF_ANNOTATE_ALL:.*:HTSLIB_BGZIPTABIX' {
+            ext.prefix = { infile.name - '.vcf' }
             publishDir = [
                     mode: params.publish_dir_mode,
                     path: { "${params.outdir}/annotation/${meta.variantcaller}/${meta.id}/" },
@@ -118,7 +118,7 @@ process {
             ]
         }
 
-        withName: 'NFCORE_SAREK:SAREK:VCF_ANNOTATE_ALL:VCF_ANNOTATE_SNPEFF:TABIX_BGZIPTABIX' {
+        withName: 'NFCORE_SAREK:SAREK:VCF_ANNOTATE_ALL:VCF_ANNOTATE_SNPEFF:HTSLIB_BGZIPTABIX' {
             publishDir = [
                 mode: params.publish_dir_mode,
                 path: { "${params.outdir}/annotation/${meta.variantcaller}/${meta.id}/" },
diff --git a/conf/modules/freebayes.config b/conf/modules/freebayes.config
index 26deda7b42..3ada1ed988 100644
--- a/conf/modules/freebayes.config
+++ b/conf/modules/freebayes.config
@@ -45,6 +45,7 @@ process {
     }
 
     withName : 'TABIX_VC_FREEBAYES' {
+        ext.prefix = { infile.baseName }
         publishDir = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/freebayes/${meta.id}/" },
@@ -63,6 +64,7 @@ process {
     }
 
     withName: 'TABIX_VC_FREEBAYES_FILT' {
+        ext.prefix = { infile.baseName }
         publishDir = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/variant_calling/freebayes/${meta.id}/" },
diff --git a/conf/modules/post_variant_calling.config b/conf/modules/post_variant_calling.config
index a42cefd668..c191b928ba 100644
--- a/conf/modules/post_variant_calling.config
+++ b/conf/modules/post_variant_calling.config
@@ -33,7 +33,7 @@ process {
     }
 
     withName: 'TABIX_EXT_VCF' {
-        ext.prefix = { "${input.baseName}" }
+        ext.prefix = { infile.baseName }
         publishDir = [ enabled: false ]
     }
 
diff --git a/conf/modules/prepare_genome.config b/conf/modules/prepare_genome.config
index cf045029f5..fd72505910 100644
--- a/conf/modules/prepare_genome.config
+++ b/conf/modules/prepare_genome.config
@@ -108,6 +108,7 @@ process {
     }
 
     withName: 'TABIX_BCFTOOLS_ANNOTATIONS' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/bcfann" },
@@ -117,6 +118,7 @@ process {
     }
 
     withName: 'TABIX_DBSNP' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/dbsnp" },
@@ -126,6 +128,7 @@ process {
     }
 
     withName: 'TABIX_GERMLINE_RESOURCE' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/germline_resource" },
@@ -135,6 +138,7 @@ process {
     }
 
     withName: 'TABIX_KNOWN_INDELS' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/known_indels" },
@@ -144,6 +148,7 @@ process {
     }
 
     withName: 'TABIX_KNOWN_SNPS' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/known_snps" },
@@ -153,6 +158,7 @@ process {
     }
 
     withName: 'TABIX_PON' {
+        ext.prefix       = { infile.baseName }
         publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/reference/pon" },
diff --git a/conf/modules/varlociraptor.config b/conf/modules/varlociraptor.config
index 575a2bf336..3b7dcbdfa7 100644
--- a/conf/modules/varlociraptor.config
+++ b/conf/modules/varlociraptor.config
@@ -136,6 +136,7 @@ process {
     }
 
     withName: '.*:VCF_VARLOCIRAPTOR_SOMATIC:TABIX_GERMLINE|.*:VCF_VARLOCIRAPTOR_SOMATIC:TABIX_SOMATIC' {
+        ext.prefix = { infile.baseName }
         publishDir = [
             enabled: false
         ]
diff --git a/main.nf b/main.nf
index 2316687d8d..dadcb67358 100755
--- a/main.nf
+++ b/main.nf
@@ -160,14 +160,12 @@ workflow NFCORE_SAREK {
         else {
             PREPARE_REFERENCE_CNVKIT(PREPARE_GENOME.out.fasta, intervals_bed_combined)
             cnvkit_reference = PREPARE_REFERENCE_CNVKIT.out.cnvkit_reference
-            versions = versions.mix(PREPARE_REFERENCE_CNVKIT.out.versions)
         }
     }
     else {
         cnvkit_reference = channel.value([])
     }
     // Gather used softwares versions
-    versions = versions.mix(PREPARE_GENOME.out.versions)
     versions = versions.mix(PREPARE_INTERVALS.out.versions)
 
     // Fails when consensus calling is specified without normalization
diff --git a/modules.json b/modules.json
index d8ee6235ab..4c47c23b6c 100644
--- a/modules.json
+++ b/modules.json
@@ -92,32 +92,32 @@
                     },
                     "cnvkit/antitarget": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/batch": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/call": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/export": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/genemetrics": {
                         "branch": "master",
-                        "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "cnvkit/reference": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "controlfreec/assesssignificance": {
@@ -346,6 +346,11 @@
                         "git_sha": "0902eac3012baaf4f9ab6513c8c55acc9353c96c",
                         "installed_by": ["modules"]
                     },
+                    "htslib/bgziptabix": {
+                        "branch": "master",
+                        "git_sha": "37f69c8ca3eb3d038a5d44f33a329e49f44bd4f2",
+                        "installed_by": ["modules", "vcf_annotate_snpeff"]
+                    },
                     "lofreq/callparallel": {
                         "branch": "master",
                         "git_sha": "9707facbacc9c5c02ecf0b192f0604a29351b00a",
@@ -546,16 +551,6 @@
                         "git_sha": "6bc8ff03ace2cb373f683b9502ce79930c8a07f0",
                         "installed_by": ["modules"]
                     },
-                    "tabix/bgziptabix": {
-                        "branch": "master",
-                        "git_sha": "23004c9c64013c90b7d835621ef4cdeff19a1427",
-                        "installed_by": ["modules", "vcf_annotate_snpeff"]
-                    },
-                    "tabix/tabix": {
-                        "branch": "master",
-                        "git_sha": "f2cfcf9d3f6a2d123e6c44aefa788aa232204a7a",
-                        "installed_by": ["modules"]
-                    },
                     "tiddit/sv": {
                         "branch": "master",
                         "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
@@ -642,7 +637,7 @@
                     },
                     "vcf_annotate_snpeff": {
                         "branch": "master",
-                        "git_sha": "23004c9c64013c90b7d835621ef4cdeff19a1427",
+                        "git_sha": "54e41f4ed3aead45054380a9befeb927612ffc91",
                         "installed_by": ["subworkflows"]
                     }
                 }
diff --git a/modules/nf-core/cnvkit/antitarget/environment.yml b/modules/nf-core/cnvkit/antitarget/environment.yml
index 9b3082be06..9d97ecfab4 100644
--- a/modules/nf-core/cnvkit/antitarget/environment.yml
+++ b/modules/nf-core/cnvkit/antitarget/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::cnvkit=0.9.11
+  - bioconda::cnvkit=0.9.12
diff --git a/modules/nf-core/cnvkit/antitarget/main.nf b/modules/nf-core/cnvkit/antitarget/main.nf
index 0c1f6674a4..7582338cac 100644
--- a/modules/nf-core/cnvkit/antitarget/main.nf
+++ b/modules/nf-core/cnvkit/antitarget/main.nf
@@ -1,18 +1,18 @@
 process CNVKIT_ANTITARGET {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.11--pyhdfd78af_0':
-        'biocontainers/cnvkit:0.9.11--pyhdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/cnvkit:0.9.12--pyhdfd78af_0'
+        : 'quay.io/biocontainers/cnvkit:0.9.12--pyhdfd78af_0'}"
 
     input:
     tuple val(meta), path(targets)
 
     output:
     tuple val(meta), path("*.bed"), emit: bed
-    path "versions.yml"           , emit: versions
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -24,13 +24,14 @@ process CNVKIT_ANTITARGET {
     """
     cnvkit.py \\
         antitarget \\
-        $targets \\
+        ${targets} \\
         --output ${prefix}.antitarget.bed \\
-        $args
+        ${args}
+    """
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e "s/cnvkit v//g")
-    END_VERSIONS
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.antitarget.bed
     """
 }
diff --git a/modules/nf-core/cnvkit/antitarget/meta.yml b/modules/nf-core/cnvkit/antitarget/meta.yml
index 13f12a10c0..e50a5ffe35 100644
--- a/modules/nf-core/cnvkit/antitarget/meta.yml
+++ b/modules/nf-core/cnvkit/antitarget/meta.yml
@@ -26,9 +26,10 @@ input:
         type: file
         description: File containing genomic regions
         pattern: "*.{bed}"
+        ontologies: []
 output:
-  - bed:
-      - meta:
+  bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -37,11 +38,28 @@ output:
           type: file
           description: File containing off-target regions
           pattern: "*.{bed}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@priesgo"
diff --git a/modules/nf-core/cnvkit/batch/environment.yml b/modules/nf-core/cnvkit/batch/environment.yml
index a2466da99f..76271e4aa5 100644
--- a/modules/nf-core/cnvkit/batch/environment.yml
+++ b/modules/nf-core/cnvkit/batch/environment.yml
@@ -5,6 +5,6 @@ channels:
   - bioconda
 
 dependencies:
-  - bioconda::cnvkit=0.9.10
-  - bioconda::htslib=1.17
-  - bioconda::samtools=1.17
+  - bioconda::cnvkit=0.9.12
+  - bioconda::htslib=1.21
+  - bioconda::samtools=1.21
diff --git a/modules/nf-core/cnvkit/batch/main.nf b/modules/nf-core/cnvkit/batch/main.nf
index 9e8aafac65..d2a7dd5a02 100644
--- a/modules/nf-core/cnvkit/batch/main.nf
+++ b/modules/nf-core/cnvkit/batch/main.nf
@@ -1,19 +1,18 @@
 process CNVKIT_BATCH {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/mulled-v2-780d630a9bb6a0ff2e7b6f730906fd703e40e98f:c94363856059151a2974dc501fb07a0360cc60a3-0' :
-        'biocontainers/mulled-v2-780d630a9bb6a0ff2e7b6f730906fd703e40e98f:c94363856059151a2974dc501fb07a0360cc60a3-0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/3e/3e8542cdb0190cfe2cedd74f714f021a2ffa94be3ec2a5b95ff52610cb3e2c34/data'
+        : 'community.wave.seqera.io/library/cnvkit_htslib_samtools:86928c121163aca7'}"
 
     input:
-    tuple val(meta), path(tumor), path(normal)
-    tuple val(meta2), path(fasta)
-    tuple val(meta3), path(fasta_fai)
+    tuple val(meta), path(tumor), path(tumor_index), path(normal), path(normal_index)
+    tuple val(meta2), path(fasta), path(fasta_fai)
     tuple val(meta4), path(targets)
     tuple val(meta5), path(reference)
-    val   panel_of_normals
+    val panel_of_normals
 
     output:
     tuple val(meta), path("*.bed"), emit: bed
@@ -22,89 +21,100 @@ process CNVKIT_BATCH {
     tuple val(meta), path("*.cns"), emit: cns, optional: true
     tuple val(meta), path("*.pdf"), emit: pdf, optional: true
     tuple val(meta), path("*.png"), emit: png, optional: true
-    path "versions.yml"           , emit: versions
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools version | sed '1!d;s/.* //'"), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
-
     script:
     def args = task.ext.args ?: ''
-
     def tumor_exists = tumor ? true : false
     def normal_exists = normal ? true : false
     def reference_exists = reference ? true : false
-
-    // execute samtools only when cram files are input, cnvkit runs natively on bam but is prohibitively slow
+    // execute samtools only when cram files are input, cnvkit runs natively on cram but is prohibitively slow
     def tumor_cram = tumor_exists && tumor.Extension == "cram" ? true : false
     def normal_cram = normal_exists && normal.Extension == "cram" ? true : false
-    def tumor_bam = tumor_exists && tumor.Extension == "bam" ? true : false
-    def normal_bam = normal_exists && normal.Extension == "bam" ? true : false
 
     def tumor_out = tumor_cram ? tumor.BaseName + ".bam" : "${tumor}"
 
     // tumor_only mode does not need fasta & target
-    // instead it requires a pre-computed reference.cnn which is built from fasta & target
+    // instead a pre-computed reference.cnn may be supplied which is built from fasta & target
     def (normal_out, normal_args, fasta_args) = ["", "", ""]
     def fai_reference = fasta_fai ? "--fai-reference ${fasta_fai}" : ""
 
-    if (normal_exists){
+    if (normal_exists) {
         def normal_prefix = normal.BaseName
         normal_out = normal_cram ? "${normal_prefix}" + ".bam" : "${normal}"
-        fasta_args = fasta ? "--fasta $fasta" : ""
+        fasta_args = fasta ? "--fasta ${fasta}" : ""
 
         // germline mode
         // normal samples must be input without a flag
         // requires flag --normal to be empty []
-        if(!tumor_exists){
+        if (!tumor_exists) {
             tumor_out = "${normal_prefix}" + ".bam"
             normal_args = "--normal "
         }
-        // somatic mode
         else {
-            normal_args = normal_prefix ? "--normal $normal_out" : ""
+            normal_args = normal_prefix ? "--normal ${normal_out}" : ""
         }
-        if (reference_exists){
+        if (reference_exists) {
             fasta_args = ""
             normal_args = ""
         }
     }
-
     // generation of panel of normals
     def generate_pon = panel_of_normals ? true : false
 
-    if (generate_pon && !tumor_exists){
+    if (generate_pon && !tumor_exists) {
         def pon_input = normal.join(' ')
-        normal_args = "--normal $pon_input"
+        normal_args = "--normal ${pon_input}"
         tumor_out = ""
     }
 
-    def target_args = targets && !reference_exists ? "--targets $targets" : ""
-    def reference_args = reference ? "--reference $reference" : ""
+    // tumor_only mode and no reference
+    // generate a "flat" reference which assumes equal coverage
+    // by passing '--normal' without any files
+    if (!reference_exists & !normal_exists & tumor_exists) {
+        normal_args = normal_args ?: "--normal"
+    }
+
+    def target_args = targets && !reference_exists ? "--targets ${targets}" : ""
+    def reference_args = reference ? "--reference ${reference}" : ""
 
     def samtools_cram_convert = ''
-    samtools_cram_convert += normal_cram ? "    samtools view -T $fasta $fai_reference $normal -@ $task.cpus -o $normal_out\n" : ''
-    samtools_cram_convert += normal_cram ? "    samtools index $normal_out\n" : ''
-    samtools_cram_convert += tumor_cram ? "    samtools view -T $fasta $fai_reference $tumor -@ $task.cpus -o $tumor_out\n" : ''
-    samtools_cram_convert += tumor_cram ? "    samtools index $tumor_out\n" : ''
-    def versions = normal_cram || tumor_cram ?
-        "samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')\n        cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')" :
-        "cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')"
+    samtools_cram_convert += normal_cram ? "    samtools view -T ${fasta} ${fai_reference} ${normal} -@ ${task.cpus} -o ${normal_out}\n" : ''
+    samtools_cram_convert += normal_cram ? "    samtools index ${normal_out}\n" : ''
+    samtools_cram_convert += tumor_cram ? "    samtools view -T ${fasta} ${fai_reference} ${tumor} -@ ${task.cpus} -o ${tumor_out}\n" : ''
+    samtools_cram_convert += tumor_cram ? "    samtools index ${tumor_out}\n" : ''
     """
-    $samtools_cram_convert
-
+    ${samtools_cram_convert}
     cnvkit.py \\
         batch \\
-        $tumor_out \\
-        $normal_args \\
-        $fasta_args \\
-        $reference_args \\
-        $target_args \\
-        --processes $task.cpus \\
-        $args
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        ${versions}
-    END_VERSIONS
+        ${tumor_out} \\
+        ${normal_args} \\
+        ${fasta_args} \\
+        ${reference_args} \\
+        ${target_args} \\
+        --processes ${task.cpus} \\
+        ${args}
+    """
+    stub:
+    def tumor_exists = tumor ? true : false
+    def reference_exists = reference ? true : false
+    // identify BED naming pattern
+    def bed_prefix = reference_exists ? reference.BaseName : targets ? targets.BaseName : ""
+    def bed_suffix = reference_exists ? "-tmp.bed" : ".bed"
+    // execute samtools only when cram files are input, cnvkit runs natively on cram but is prohibitively slow
+    def out_base_name = tumor_exists ? tumor.BaseName : normal.BaseName
+    """
+    touch ${bed_prefix}.antitarget${bed_suffix}
+    touch ${bed_prefix}.target${bed_suffix}
+    touch "reference.cnn"
+    touch ${out_base_name}.antitargetcoverage.cnn
+    touch ${out_base_name}.bintest.cns
+    touch ${out_base_name}.call.cns
+    touch ${out_base_name}.cnr
+    touch ${out_base_name}.cns
+    touch ${out_base_name}.targetcoverage.cnn
     """
 }
diff --git a/modules/nf-core/cnvkit/batch/meta.yml b/modules/nf-core/cnvkit/batch/meta.yml
index 30f7a1a29b..622f76af10 100644
--- a/modules/nf-core/cnvkit/batch/meta.yml
+++ b/modules/nf-core/cnvkit/batch/meta.yml
@@ -11,7 +11,8 @@ tools:
         CNVkit is a Python library and command-line software toolkit to infer and visualize copy number from high-throughput DNA sequencing data. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.
       homepage: https://cnvkit.readthedocs.io/en/stable/index.html
       documentation: https://cnvkit.readthedocs.io/en/stable/index.html
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: biotools:cnvkit
 input:
   - - meta:
@@ -23,10 +24,32 @@ input:
         type: file
         description: |
           Input tumour sample bam file (or cram)
+        pattern: "*.{bam,cram}"
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+          - edam: http://edamontology.org/format_3462 # CRAM
+    - tumor_index:
+        type: file
+        description: |
+          Input tumour sample bam/cram index file (only needed for bam input)
+        pattern: "*.{bai,crai}"
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # BAI
     - normal:
         type: file
         description: |
           Input normal sample bam file (or cram)
+        pattern: "*.{bam,cram}"
+        ontologies:
+          - edam: http://edamontology.org/format_2572 # BAM
+          - edam: http://edamontology.org/format_3462 # CRAM
+    - normal_index:
+        type: file
+        description: |
+          Input normal sample bam/cram index file (only needed for bam input)
+        pattern: "*.{bai,crai}"
+        ontologies:
+          - edam: http://edamontology.org/format_3327 # BAI
   - - meta2:
         type: map
         description: |
@@ -36,15 +59,15 @@ input:
         type: file
         description: |
           Input reference genome fasta file (only needed for cram_input and/or when normal_samples are provided)
-  - - meta3:
-        type: map
-        description: |
-          Groovy Map containing reference information
-          e.g. [ id:'test' ]
+        pattern: "*.{fa,fasta}"
+        ontologies:
+          - edam: http://edamontology.org/format_1929 # FASTA
     - fasta_fai:
         type: file
         description: |
           Input reference genome fasta index (optional, but recommended for cram_input)
+        pattern: "*.{fai}"
+        ontologies: []
   - - meta4:
         type: map
         description: |
@@ -54,6 +77,9 @@ input:
         type: file
         description: |
           Input target bed file
+        pattern: "*.{bed}"
+        ontologies:
+          - edam: http://edamontology.org/format_3003 # BED
   - - meta5:
         type: map
         description: |
@@ -63,13 +89,17 @@ input:
         type: file
         description: |
           Input reference cnn-file (only for germline and tumor-only running)
-  - - panel_of_normals:
-        type: file
-        description: |
-          Input panel of normals file
+        pattern: "*.{cnn}"
+        ontologies: []
+  - panel_of_normals:
+      type: file
+      description: |
+        Input panel of normals file
+      pattern: "*.{cnn}"
+      ontologies: []
 output:
-  - bed:
-      - meta:
+  bed:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -78,8 +108,10 @@ output:
           type: file
           description: File containing genomic regions
           pattern: "*.{bed}"
-  - cnn:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3003 # BED
+  cnn:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -88,8 +120,9 @@ output:
           type: file
           description: File containing coverage information
           pattern: "*.{cnn}"
-  - cnr:
-      - meta:
+          ontologies: []
+  cnr:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -98,8 +131,9 @@ output:
           type: file
           description: File containing copy number ratio information
           pattern: "*.{cnr}"
-  - cns:
-      - meta:
+          ontologies: []
+  cns:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -108,8 +142,9 @@ output:
           type: file
           description: File containing copy number segment information
           pattern: "*.{cns}"
-  - pdf:
-      - meta:
+          ontologies: []
+  pdf:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -118,21 +153,61 @@ output:
           type: file
           description: File with plot of copy numbers or segments on chromosomes
           pattern: "*.{pdf}"
-  - png:
-      - meta:
+          ontologies:
+            - edam: http://edamontology.org/format_3508 # PDF
+  png:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
             e.g. [ id:'test', single_end:false ]
       - "*.png":
           type: file
-          description: File with plot of bin-level log2 coverages and segmentation calls
+          description: File with plot of bin-level log2 coverages and segmentation
+            calls
           pattern: "*.{png}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3603 # PNG
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_samtools:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - samtools version | sed '1!d;s/.* //':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@drpatelh"
diff --git a/modules/nf-core/cnvkit/call/environment.yml b/modules/nf-core/cnvkit/call/environment.yml
index 690d8fd7f7..9d97ecfab4 100644
--- a/modules/nf-core/cnvkit/call/environment.yml
+++ b/modules/nf-core/cnvkit/call/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::cnvkit=0.9.10
+  - bioconda::cnvkit=0.9.12
diff --git a/modules/nf-core/cnvkit/call/main.nf b/modules/nf-core/cnvkit/call/main.nf
index 06d51e857e..161c3943a6 100644
--- a/modules/nf-core/cnvkit/call/main.nf
+++ b/modules/nf-core/cnvkit/call/main.nf
@@ -1,18 +1,18 @@
 process CNVKIT_CALL {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.10--pyhdfd78af_0':
-        'biocontainers/cnvkit:0.9.10--pyhdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/cnvkit:0.9.12--pyhdfd78af_0'
+        : 'quay.io/biocontainers/cnvkit:0.9.12--pyhdfd78af_0'}"
 
     input:
-    tuple val(meta) , path(cns), path(vcf)
+    tuple val(meta), path(cns), path(vcf)
 
     output:
     tuple val(meta), path("*.cns"), emit: cns
-    path "versions.yml"           , emit: versions
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -20,28 +20,18 @@ process CNVKIT_CALL {
     script:
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
-    def vcf_cmd = vcf ? "-v $vcf" : ""
+    def vcf_cmd = vcf ? "-v ${vcf}" : ""
     """
     cnvkit.py call \\
-        $cns \\
-        $vcf_cmd \\
-        $args \\
+        ${cns} \\
+        ${vcf_cmd} \\
+        ${args} \\
         -o ${prefix}.cns
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')
-    END_VERSIONS
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
     touch ${prefix}.cns
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/cnvkit/call/meta.yml b/modules/nf-core/cnvkit/call/meta.yml
index b3b4a4a78a..d33147e152 100644
--- a/modules/nf-core/cnvkit/call/meta.yml
+++ b/modules/nf-core/cnvkit/call/meta.yml
@@ -1,6 +1,6 @@
 name: cnvkit_call
-description: Given segmented log2 ratio estimates (.cns), derive each segment’s absolute
-  integer copy number
+description: Given segmented log2 ratio estimates (.cns), derive each segment’s
+  absolute integer copy number
 keywords:
   - cnvkit
   - bam
@@ -12,7 +12,8 @@ tools:
         CNVkit is a Python library and command-line software toolkit to infer and visualize copy number from high-throughput DNA sequencing data. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.
       homepage: https://cnvkit.readthedocs.io/en/stable/index.html
       documentation: https://cnvkit.readthedocs.io/en/stable/index.html
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: biotools:cnvkit
 input:
   - - meta:
@@ -24,13 +25,15 @@ input:
         type: file
         description: CNVKit CNS file.
         pattern: "*.cns"
+        ontologies: []
     - vcf:
         type: file
         description: Germline VCF file for BAF.
         pattern: "*.vcf{,.gz}"
+        ontologies: []
 output:
-  - cns:
-      - meta:
+  cns:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -39,11 +42,28 @@ output:
           type: file
           description: CNS file.
           pattern: "*.cns"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@priesgo"
diff --git a/modules/nf-core/cnvkit/export/environment.yml b/modules/nf-core/cnvkit/export/environment.yml
index 690d8fd7f7..9d97ecfab4 100644
--- a/modules/nf-core/cnvkit/export/environment.yml
+++ b/modules/nf-core/cnvkit/export/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::cnvkit=0.9.10
+  - bioconda::cnvkit=0.9.12
diff --git a/modules/nf-core/cnvkit/export/main.nf b/modules/nf-core/cnvkit/export/main.nf
index d1d7d3415b..a36abe1047 100644
--- a/modules/nf-core/cnvkit/export/main.nf
+++ b/modules/nf-core/cnvkit/export/main.nf
@@ -1,18 +1,18 @@
 process CNVKIT_EXPORT {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.10--pyhdfd78af_0':
-        'biocontainers/cnvkit:0.9.10--pyhdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/cnvkit:0.9.12--pyhdfd78af_0'
+        : 'quay.io/biocontainers/cnvkit:0.9.12--pyhdfd78af_0'}"
 
     input:
     tuple val(meta), path(cns)
 
     output:
     tuple val(meta), path("${prefix}.${suffix}"), emit: output
-    path "versions.yml"                         , emit: versions
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -23,14 +23,9 @@ process CNVKIT_EXPORT {
     suffix = task.ext.args.tokenize(" ")[0]
     """
     cnvkit.py export \\
-        $args \\
-        $cns \\
+        ${args} \\
+        ${cns} \\
         -o ${prefix}.${suffix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')
-    END_VERSIONS
     """
 
     stub:
@@ -38,10 +33,5 @@ process CNVKIT_EXPORT {
     suffix = task.ext.args.tokenize(" ")[0]
     """
     touch ${prefix}.${suffix}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e 's/cnvkit v//g')
-    END_VERSIONS
     """
 }
diff --git a/modules/nf-core/cnvkit/export/meta.yml b/modules/nf-core/cnvkit/export/meta.yml
index d37e41f98f..4d6441b985 100644
--- a/modules/nf-core/cnvkit/export/meta.yml
+++ b/modules/nf-core/cnvkit/export/meta.yml
@@ -1,6 +1,6 @@
 name: cnvkit_export
-description: Convert copy number ratio tables (.cnr files) or segments (.cns) to another
-  format.
+description: Convert copy number ratio tables (.cnr files) or segments (.cns) to
+  another format.
 keywords:
   - cnvkit
   - copy number
@@ -14,7 +14,8 @@ tools:
         target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.
       homepage: https://cnvkit.readthedocs.io/en/stable/index.html
       documentation: https://cnvkit.readthedocs.io/en/stable/index.html
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: biotools:cnvkit
 input:
   - - meta:
@@ -26,9 +27,10 @@ input:
         type: file
         description: CNVKit CNS file.
         pattern: "*.cns"
+        ontologies: []
 output:
-  - output:
-      - meta:
+  output:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -36,11 +38,28 @@ output:
       - ${prefix}.${suffix}:
           type: file
           description: Output file
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies: []
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@priesgo"
diff --git a/modules/nf-core/cnvkit/genemetrics/environment.yml b/modules/nf-core/cnvkit/genemetrics/environment.yml
index 690d8fd7f7..9d97ecfab4 100644
--- a/modules/nf-core/cnvkit/genemetrics/environment.yml
+++ b/modules/nf-core/cnvkit/genemetrics/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::cnvkit=0.9.10
+  - bioconda::cnvkit=0.9.12
diff --git a/modules/nf-core/cnvkit/genemetrics/main.nf b/modules/nf-core/cnvkit/genemetrics/main.nf
index 825b12bdac..252e36043f 100644
--- a/modules/nf-core/cnvkit/genemetrics/main.nf
+++ b/modules/nf-core/cnvkit/genemetrics/main.nf
@@ -1,19 +1,18 @@
 process CNVKIT_GENEMETRICS {
-    tag "$meta.id"
+    tag "${meta.id}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.10--pyhdfd78af_0':
-        'biocontainers/cnvkit:0.9.10--pyhdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/cnvkit:0.9.12--pyhdfd78af_0'
+        : 'quay.io/biocontainers/cnvkit:0.9.12--pyhdfd78af_0'}"
 
     input:
     tuple val(meta), path(cnr), path(cns)
 
     output:
     tuple val(meta), path("*.tsv"), emit: tsv
-    //tuple val(meta), path("*.cnn"), emit: cnn
-    path "versions.yml"           , emit: versions
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,14 +25,16 @@ process CNVKIT_GENEMETRICS {
     """
     cnvkit.py \\
         genemetrics \\
-        $cnr \\
-        $segments \\
+        ${cnr} \\
+        ${segments} \\
         --output ${prefix}.tsv \\
-        $args
+        ${args}
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: "${meta.id}"
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e "s/cnvkit v//g")
-    END_VERSIONS
+    """
+    touch ${prefix}.tsv
     """
 }
diff --git a/modules/nf-core/cnvkit/genemetrics/meta.yml b/modules/nf-core/cnvkit/genemetrics/meta.yml
index 6b110accc2..34595e308e 100644
--- a/modules/nf-core/cnvkit/genemetrics/meta.yml
+++ b/modules/nf-core/cnvkit/genemetrics/meta.yml
@@ -11,7 +11,8 @@ tools:
         CNVkit is a Python library and command-line software toolkit to infer and visualize copy number from high-throughput DNA sequencing data. It is designed for use with hybrid capture, including both whole-exome and custom target panels, and short-read sequencing platforms such as Illumina and Ion Torrent.
       homepage: https://cnvkit.readthedocs.io/en/stable/index.html
       documentation: https://cnvkit.readthedocs.io/en/stable/index.html
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: biotools:cnvkit
 input:
   - - meta:
@@ -23,13 +24,15 @@ input:
         type: file
         description: CNR file
         pattern: "*.cnr"
+        ontologies: []
     - cns:
         type: file
         description: CNS file [Optional]
         pattern: "*.cns"
+        ontologies: []
 output:
-  - tsv:
-      - meta:
+  tsv:
+    - - meta:
           type: map
           description: |
             Groovy Map containing sample information
@@ -38,21 +41,29 @@ output:
           type: file
           description: TSV file
           pattern: "*.tsv"
-  - cnn:
-      - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.cnn":
-          type: file
-          description: CNN file
-          pattern: "*.cnn"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+          ontologies:
+            - edam: http://edamontology.org/format_3475
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@marrip"
diff --git a/modules/nf-core/cnvkit/reference/environment.yml b/modules/nf-core/cnvkit/reference/environment.yml
index 9b3082be06..9d97ecfab4 100644
--- a/modules/nf-core/cnvkit/reference/environment.yml
+++ b/modules/nf-core/cnvkit/reference/environment.yml
@@ -4,4 +4,4 @@ channels:
   - conda-forge
   - bioconda
 dependencies:
-  - bioconda::cnvkit=0.9.11
+  - bioconda::cnvkit=0.9.12
diff --git a/modules/nf-core/cnvkit/reference/main.nf b/modules/nf-core/cnvkit/reference/main.nf
index 857996f111..e1de0fffd7 100644
--- a/modules/nf-core/cnvkit/reference/main.nf
+++ b/modules/nf-core/cnvkit/reference/main.nf
@@ -1,11 +1,11 @@
 process CNVKIT_REFERENCE {
-    tag "$fasta"
+    tag "${fasta}"
     label 'process_low'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://depot.galaxyproject.org/singularity/cnvkit:0.9.11--pyhdfd78af_0':
-        'biocontainers/cnvkit:0.9.11--pyhdfd78af_0' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/cnvkit:0.9.12--pyhdfd78af_0'
+        : 'quay.io/biocontainers/cnvkit:0.9.12--pyhdfd78af_0'}"
 
     input:
     path fasta
@@ -13,8 +13,8 @@ process CNVKIT_REFERENCE {
     path antitargets
 
     output:
-    path "*.cnn"       , emit: cnn
-    path "versions.yml", emit: versions
+    path "*.cnn",        emit: cnn
+    tuple val("${task.process}"), val('cnvkit'), eval('cnvkit.py version | sed -e "s/cnvkit v//g"'), emit: versions_cnvkit, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -26,15 +26,17 @@ process CNVKIT_REFERENCE {
     """
     cnvkit.py \\
         reference \\
-        --fasta $fasta \\
-        --targets $targets \\
-        --antitargets $antitargets \\
+        --fasta ${fasta} \\
+        --targets ${targets} \\
+        --antitargets ${antitargets} \\
         --output ${prefix}.reference.cnn \\
-        $args
+        ${args}
+    """
+
+    stub:
+    def prefix = task.ext.prefix ?: targets.BaseName
 
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        cnvkit: \$(cnvkit.py version | sed -e "s/cnvkit v//g")
-    END_VERSIONS
+    """
+    touch ${prefix}.reference.cnn
     """
 }
diff --git a/modules/nf-core/cnvkit/reference/meta.yml b/modules/nf-core/cnvkit/reference/meta.yml
index 965a7b5795..87b124eb8a 100644
--- a/modules/nf-core/cnvkit/reference/meta.yml
+++ b/modules/nf-core/cnvkit/reference/meta.yml
@@ -14,33 +14,54 @@ tools:
       documentation: https://cnvkit.readthedocs.io/en/stable/index.html
       tool_dev_url: https://github.com/etal/cnvkit
       doi: 10.1371/journal.pcbi.1004873
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: biotools:cnvkit
 input:
-  - - fasta:
-        type: file
-        description: File containing reference genome
-        pattern: "*.{fasta}"
-  - - targets:
-        type: file
-        description: File containing genomic regions
-        pattern: "*.{bed}"
-  - - antitargets:
-        type: file
-        description: File containing off-target genomic regions
-        pattern: "*.{bed}"
+  - fasta:
+      type: file
+      description: File containing reference genome
+      pattern: "*.{fasta}"
+      ontologies: []
+  - targets:
+      type: file
+      description: File containing genomic regions
+      pattern: "*.{bed}"
+      ontologies: []
+  - antitargets:
+      type: file
+      description: File containing off-target genomic regions
+      pattern: "*.{bed}"
+      ontologies: []
 output:
-  - cnn:
-      - "*.cnn":
-          type: file
-          description: File containing a copy-number reference (required for CNV calling
-            in tumor_only mode)
-          pattern: "*.{cnn}"
-  - versions:
-      - versions.yml:
-          type: file
-          description: File containing software versions
-          pattern: "versions.yml"
+  cnn:
+    - "*.cnn":
+        type: file
+        description: File containing a copy-number reference (required for CNV
+          calling in tumor_only mode)
+        pattern: "*.{cnn}"
+        ontologies: []
+  versions_cnvkit:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - cnvkit:
+          type: string
+          description: The name of the tool
+      - 'cnvkit.py version | sed -e "s/cnvkit v//g"':
+          type: eval
+          description: The expression to obtain the version of the tool
 authors:
   - "@adamrtalbot"
   - "@priesgo"
diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/htslib/bgziptabix/environment.yml
similarity index 77%
rename from modules/nf-core/tabix/bgziptabix/environment.yml
rename to modules/nf-core/htslib/bgziptabix/environment.yml
index 771b138707..ec62c057d7 100644
--- a/modules/nf-core/tabix/bgziptabix/environment.yml
+++ b/modules/nf-core/htslib/bgziptabix/environment.yml
@@ -3,6 +3,6 @@
 channels:
   - conda-forge
   - bioconda
-
 dependencies:
-  - bioconda::htslib=1.21
+  - bioconda::htslib=1.24
+  - conda-forge::xz=5.8.3
diff --git a/modules/nf-core/htslib/bgziptabix/main.nf b/modules/nf-core/htslib/bgziptabix/main.nf
new file mode 100644
index 0000000000..573c05fcdc
--- /dev/null
+++ b/modules/nf-core/htslib/bgziptabix/main.nf
@@ -0,0 +1,88 @@
+process HTSLIB_BGZIPTABIX {
+    tag "${meta.id}"
+    label 'process_low'
+
+    conda "${moduleDir}/environment.yml"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/86/863ca0dbbba30c8367fa4fbd3fa3a84393532fb7b300a5c5c2e70f0dfc475bbf/data'
+        : 'community.wave.seqera.io/library/htslib_xz:32f2772a564b3cd2'}"
+
+    input:
+    tuple val(meta), path(infile), path(infile_tbi), path(regions)
+    val action
+    val make_index
+    val out_ext
+
+    output:
+    tuple val(meta), path("${outfile}"), emit: output
+    tuple val(meta), path("${outfile}.{tbi,csi}"), emit: index, optional: true
+    // all htslib tools have the same version, we use bgzip
+    tuple val("${task.process}"), val('htslib'), eval("bgzip --version | sed '1! d; s/bgzip (htslib) //'"), topic: versions, emit: versions_htslib
+    tuple val("${task.process}"), val('xz'), eval("xz --version | sed '1! d; s/xz (XZ Utils) //'"), topic: versions, emit: versions_xz
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def allowed_actions = ["compress", "decompress"]
+    if (action !in allowed_actions) {
+        error("htslib/bgziptabix: Invalid action: ${action}. Allowed actions are: ${allowed_actions.join(', ')}")
+    }
+
+    if (action == "decompress" && make_index) {
+        log.warn("htslib/bgziptabix: Cannot create index when decompressing. Ignoring make_index option.")
+    }
+
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    outfile = action == "compress" ? (out_ext ? "${prefix}.${out_ext}.gz" : "${prefix}.gz") : (out_ext ? "${prefix}.${out_ext}" : "${prefix}")
+
+    def compress_cmd = action == "compress" ? "bgzip -c ${args} -@ ${task.cpus}" : "cat"
+    def bgzip_cmd = action == "compress" ? "[ '\$(basename ${infile})' != '\$(basename ${outfile})' ] && ln -s ${infile} ${outfile}" : "bgzip -c -d ${args} -@ ${task.cpus} ${infile} > ${outfile}"
+
+    def regions_arg = regions ? "-R ${regions}" : ""
+    def tabix_cmd = (make_index && !infile_tbi) ? "tabix -@ ${task.cpus} ${regions_arg} ${args2} -f ${outfile}" : ""
+    def link_tabix_cmd = make_index && infile_tbi ? "ln -s ${infile_tbi} ${outfile}.${infile_tbi.extension}" : ""
+    def uncompressed_cmd = action == "compress" ? "${compress_cmd} ${infile} > ${outfile}" : (infile.getName() == outfile ? "" : "ln -s ${infile} ${outfile}")
+    """
+    ${link_tabix_cmd}
+
+    FILE_TYPE=\$(htsfile ${infile})
+
+    case "\$FILE_TYPE" in
+        *BGZF-compressed*)
+            ${bgzip_cmd} ;;
+        *gzip-compressed*)
+            [ "\$(basename ${infile})" == "\$(basename ${outfile})" ] && echo "Input and output names cannot be the same" && exit 1
+            bgzip -d -c -@ ${task.cpus} ${infile} | ${compress_cmd} > ${outfile} ;;
+        *bzip2-compressed*)
+            bzcat ${infile} | ${compress_cmd} > ${outfile} ;;
+        *XZ-compressed*)
+            xzcat ${infile} | ${compress_cmd} > ${outfile} ;;
+        *)
+            ${uncompressed_cmd} ;;
+    esac
+
+    ${tabix_cmd}
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def args2 = task.ext.args2 ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    outfile = action == "compress" ? (out_ext ? "${prefix}.${out_ext}.gz" : "${prefix}.gz") : (out_ext ? "${prefix}.${out_ext}" : "${prefix}")
+
+    def touch_cmd = action == "compress" ? "echo | bgzip -c" : "echo"
+    def index_fmt = args2.contains('-C') ? 'csi' : 'tbi'
+    def tabix_cmd = make_index ? "touch ${outfile}.${index_fmt}" : ""
+    def link_tabix_cmd = make_index && infile_tbi ? "ln -s ${infile_tbi} ${outfile}.${infile_tbi.extension}" : ""
+    """
+    echo ${args}
+
+    ${touch_cmd} > ${outfile}
+
+    ${tabix_cmd}
+    ${link_tabix_cmd}
+    """
+}
diff --git a/modules/nf-core/htslib/bgziptabix/meta.yml b/modules/nf-core/htslib/bgziptabix/meta.yml
new file mode 100644
index 0000000000..4cdefd0e16
--- /dev/null
+++ b/modules/nf-core/htslib/bgziptabix/meta.yml
@@ -0,0 +1,125 @@
+name: "htslib_bgziptabix"
+description: "Multi-purpose module to compress, decompress and index files using bgzip
+  and tabix."
+keywords:
+  - compress
+  - decompress
+  - index
+  - bgzip
+  - tabix
+  - gzip
+  - bzip
+  - xz
+tools:
+  - "htslib":
+      description: "C library for high-throughput sequencing data formats."
+      homepage: "http://www.htslib.org/"
+      documentation: "http://www.htslib.org/doc/"
+      tool_dev_url: "https://github.com/samtools/htslib"
+      doi: "10.1093/gigascience/giab007"
+      licence:
+        - "MIT"
+      identifier: biotools:htslib
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'sample1' ]
+    - infile:
+        type: file
+        description: Input file to compress or decompress
+        pattern: "*"
+        ontologies: []
+    - infile_tbi:
+        type: file
+        description: Optional tabix index for the input file.
+        pattern: "*.{tbi,csi}"
+        ontologies:
+          - edam: http://edamontology.org/format_3616 # tabix
+    - regions:
+        type: file
+        description: Optional file of regions to extract (BED or chr:start-end format).
+          Only used when creating an index for the output file.
+        pattern: "*.{bed,txt,tsv}"
+        ontologies:
+          - edam: http://edamontology.org/format_3475 # TSV
+          - edam: http://edamontology.org/format_3003 # BED
+  - action:
+      type: string
+      description: Action to perform, either `compress` or `decompress`
+  - make_index:
+      type: boolean
+      description: Whether to create a tabix index for the output file; only used
+        if `action` is `compress`
+  - out_ext:
+      type: string
+      description: Output file extension without `.gz` suffix (for example `vcf`)
+output:
+  output:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'sample1' ]
+      - ${outfile}:
+          type: file
+          description: Compressed or decompressed output file
+          pattern: "*"
+          ontologies: []
+  index:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'sample1' ]
+      - ${outfile}.{tbi,csi}:
+          type: file
+          description: Tabix index file for the compressed output file
+          pattern: "*.{tbi,csi}"
+          ontologies:
+            - edam: http://edamontology.org/format_3616 # tabix
+  versions_htslib:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - htslib:
+          type: string
+          description: The name of the tool
+      - bgzip --version | sed '1! d; s/bgzip (htslib) //':
+          type: eval
+          description: The expression to obtain the version of the tool
+  versions_xz:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - xz:
+          type: string
+          description: The name of the tool
+      - xz --version | sed '1! d; s/xz (XZ Utils) //':
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - htslib:
+          type: string
+          description: The name of the tool
+      - bgzip --version | sed '1! d; s/bgzip (htslib) //':
+          type: eval
+          description: The expression to obtain the version of the tool
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - xz:
+          type: string
+          description: The name of the tool
+      - xz --version | sed '1! d; s/xz (XZ Utils) //':
+          type: eval
+          description: The expression to obtain the version of the tool
+authors:
+  - "@itrujnara"
+maintainers:
+  - "@itrujnara"
diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf
deleted file mode 100644
index 30eae745fc..0000000000
--- a/modules/nf-core/tabix/bgziptabix/main.nf
+++ /dev/null
@@ -1,40 +0,0 @@
-process TABIX_BGZIPTABIX {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
-        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
-
-    input:
-    tuple val(meta), path(input)
-
-    output:
-    tuple val(meta), path("*.gz"), path("*.{tbi,csi}"), emit: gz_index
-    tuple val("${task.process}"), val('tabix'), eval("tabix -h 2>&1 | grep -oP 'Version:\\s*\\K[^\\s]+'")   , topic: versions   , emit: versions_tabix
-    tuple val("${task.process}"), val('bgzip'), eval("bgzip --version | sed '1!d;s/.* //'"), topic: versions, emit: versions_bgzip
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    def args2 = task.ext.args2 ?: ''
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    """
-    bgzip --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz
-    tabix --threads ${task.cpus} $args2 ${prefix}.${input.getExtension()}.gz
-
-    """
-
-    stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    def args2 = task.ext.args2 ?: ''
-    def index = args2.contains("-C ") || args2.contains("--csi") ? "csi" : "tbi"
-    """
-    echo "" | gzip > ${prefix}.${input.getExtension()}.gz
-    touch ${prefix}.${input.getExtension()}.gz.${index}
-
-    """
-}
diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml
deleted file mode 100644
index 2a3078c55f..0000000000
--- a/modules/nf-core/tabix/bgziptabix/meta.yml
+++ /dev/null
@@ -1,93 +0,0 @@
-name: tabix_bgziptabix
-description: bgzip a sorted tab-delimited genome file and then create tabix
-  index
-keywords:
-  - bgzip
-  - compress
-  - index
-  - tabix
-  - vcf
-tools:
-  - tabix:
-      description: Generic indexer for TAB-delimited genome position files.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: https://www.htslib.org/doc/tabix.1.html
-      doi: 10.1093/bioinformatics/btq671
-      licence: ["MIT"]
-      identifier: biotools:tabix
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - input:
-        type: file
-        description: Sorted tab-delimited genome file
-        ontologies: []
-output:
-  gz_index:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.gz":
-          type: file
-          description: bgzipped tab-delimited genome file
-          pattern: "*.gz"
-          ontologies:
-            - edam: http://edamontology.org/format_3989 # GZIP format
-      - "*.{tbi,csi}":
-          type: file
-          description: Tabix index file (either tbi or csi)
-          pattern: "*.{tbi,csi}"
-          ontologies: []
-  versions_tabix:
-    - - ${task.process}:
-          type: string
-          description: The process the versions were collected from
-      - tabix:
-          type: string
-          description: The tool name
-      - tabix -h 2>&1 | grep -oP 'Version:\s*\K[^\s]+':
-          type: eval
-          description: The expression to obtain the version of the tool
-  versions_bgzip:
-    - - ${task.process}:
-          type: string
-          description: The process the versions were collected from
-      - bgzip:
-          type: string
-          description: The tool name
-      - bgzip --version | sed '1!d;s/.* //':
-          type: eval
-          description: The expression to obtain the version of the tool
-
-topics:
-  versions:
-    - - ${task.process}:
-          type: string
-          description: The process the versions were collected from
-      - tabix:
-          type: string
-          description: The tool name
-      - tabix -h 2>&1 | grep -oP 'Version:\s*\K[^\s]+':
-          type: eval
-          description: The expression to obtain the version of the tool
-    - - ${task.process}:
-          type: string
-          description: The process the versions were collected from
-      - bgzip:
-          type: string
-          description: The tool name
-      - bgzip --version | sed '1!d;s/.* //':
-          type: eval
-          description: The expression to obtain the version of the tool
-
-authors:
-  - "@maxulysse"
-  - "@DLBPointon"
-maintainers:
-  - "@maxulysse"
-  - "@DLBPointon"
diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml
deleted file mode 100644
index 771b138707..0000000000
--- a/modules/nf-core/tabix/tabix/environment.yml
+++ /dev/null
@@ -1,8 +0,0 @@
----
-# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
-channels:
-  - conda-forge
-  - bioconda
-
-dependencies:
-  - bioconda::htslib=1.21
diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf
deleted file mode 100644
index 325b8bbff8..0000000000
--- a/modules/nf-core/tabix/tabix/main.nf
+++ /dev/null
@@ -1,45 +0,0 @@
-process TABIX_TABIX {
-    tag "$meta.id"
-    label 'process_single'
-
-    conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/92/92859404d861ae01afb87e2b789aebc71c0ab546397af890c7df74e4ee22c8dd/data' :
-        'community.wave.seqera.io/library/htslib:1.21--ff8e28a189fbecaa' }"
-
-    input:
-    tuple val(meta), path(tab)
-
-    output:
-    tuple val(meta), path("*.tbi"), optional:true, emit: tbi
-    tuple val(meta), path("*.csi"), optional:true, emit: csi
-    path  "versions.yml"          , emit: versions
-
-    when:
-    task.ext.when == null || task.ext.when
-
-    script:
-    def args = task.ext.args ?: ''
-    """
-    tabix \\
-        --threads $task.cpus \\
-        $args \\
-        $tab
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-
-    stub:
-    """
-    touch ${tab}.tbi
-    touch ${tab}.csi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//')
-    END_VERSIONS
-    """
-}
diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml
deleted file mode 100644
index c172968d87..0000000000
--- a/modules/nf-core/tabix/tabix/meta.yml
+++ /dev/null
@@ -1,63 +0,0 @@
-name: tabix_tabix
-description: create tabix index from a sorted bgzip tab-delimited genome file
-keywords:
-  - index
-  - tabix
-  - vcf
-tools:
-  - tabix:
-      description: Generic indexer for TAB-delimited genome position files.
-      homepage: https://www.htslib.org/doc/tabix.html
-      documentation: https://www.htslib.org/doc/tabix.1.html
-      doi: 10.1093/bioinformatics/btq671
-      licence: ["MIT"]
-      identifier: biotools:tabix
-input:
-  - - meta:
-        type: map
-        description: |
-          Groovy Map containing sample information
-          e.g. [ id:'test', single_end:false ]
-    - tab:
-        type: file
-        description: TAB-delimited genome position file compressed with bgzip
-        pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}"
-        ontologies: []
-output:
-  tbi:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.tbi":
-          type: file
-          description: tabix index file
-          pattern: "*.{tbi}"
-          ontologies: []
-  csi:
-    - - meta:
-          type: map
-          description: |
-            Groovy Map containing sample information
-            e.g. [ id:'test', single_end:false ]
-      - "*.csi":
-          type: file
-          description: coordinate sorted index file
-          pattern: "*.{csi}"
-          ontologies: []
-  versions:
-    - versions.yml:
-        type: file
-        description: File containing software versions
-        pattern: "versions.yml"
-        ontologies:
-          - edam: http://edamontology.org/format_3750 # YAML
-authors:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
-maintainers:
-  - "@joseespinosa"
-  - "@drpatelh"
-  - "@maxulysse"
diff --git a/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf b/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
index a364876c6b..775aca85be 100644
--- a/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
+++ b/subworkflows/local/bam_joint_calling_germline_sentieon/main.nf
@@ -30,8 +30,6 @@ workflow BAM_JOINT_CALLING_GERMLINE_SENTIEON {
     variant_caller
 
     main:
-    versions = channel.empty()
-
     sentieon_input = input
         .map{ meta, gvcf, tbi, intervals -> [ [ id:'joint_variant_calling', intervals_name:intervals.baseName, num_intervals:meta.num_intervals ], gvcf, tbi, intervals ] }
         .groupTuple(by:[0, 3])
@@ -139,6 +137,4 @@ workflow BAM_JOINT_CALLING_GERMLINE_SENTIEON {
     emit:
     genotype_index  // channel: [ val(meta), [ tbi ] ]
     genotype_vcf    // channel: [ val(meta), [ vcf ] ]
-
-    versions        // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_variant_calling_cnvkit/main.nf b/subworkflows/local/bam_variant_calling_cnvkit/main.nf
index 2567f0042c..2e969a520a 100644
--- a/subworkflows/local/bam_variant_calling_cnvkit/main.nf
+++ b/subworkflows/local/bam_variant_calling_cnvkit/main.nf
@@ -18,10 +18,14 @@ workflow BAM_VARIANT_CALLING_CNVKIT {
     reference           // channel: [optional]  meta, cnn
 
     main:
-    versions = channel.empty()
     generate_pon = false
 
-    CNVKIT_BATCH(cram, fasta, fasta_fai, targets, reference, generate_pon)
+    // tumor/normal index slots are unused (CNVKIT_BATCH indexes CRAMs internally)
+    cram_input = cram.map { meta, tumor, normal -> [meta, tumor, [], normal, []] }
+
+    fasta_input = fasta.combine(fasta_fai).map { meta, fasta_, _meta_fai, fasta_fai_ -> [meta, fasta_, fasta_fai_] }
+
+    CNVKIT_BATCH(cram_input, fasta_input, targets, reference, generate_pon)
 
     // right now we do not use an input VCF to improve the calling of B alleles
     // based on SNV frequencies from the VCF file
@@ -35,12 +39,7 @@ workflow BAM_VARIANT_CALLING_CNVKIT {
     ch_genemetrics = CNVKIT_BATCH.out.cnr.join(CNVKIT_BATCH.out.cns).map{ meta, cnr, cns -> [meta, cnr, cns[2]]}
     CNVKIT_GENEMETRICS(ch_genemetrics)
 
-    versions = versions.mix(CNVKIT_BATCH.out.versions)
-    versions = versions.mix(CNVKIT_GENEMETRICS.out.versions)
-    versions = versions.mix(CNVKIT_CALL.out.versions)
-    versions = versions.mix(CNVKIT_EXPORT.out.versions)
     emit:
     cnv_calls_raw    = CNVKIT_CALL.out.cns      // channel: [ meta, cns ]
     cnv_calls_export = CNVKIT_EXPORT.out.output // channel: [ meta, export_format ]
-    versions                                    // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_variant_calling_freebayes/main.nf b/subworkflows/local/bam_variant_calling_freebayes/main.nf
index 9491697a8a..b859ade32d 100644
--- a/subworkflows/local/bam_variant_calling_freebayes/main.nf
+++ b/subworkflows/local/bam_variant_calling_freebayes/main.nf
@@ -7,8 +7,8 @@
 include { BCFTOOLS_SORT                              } from '../../../modules/nf-core/bcftools/sort'
 include { FREEBAYES                                  } from '../../../modules/nf-core/freebayes'
 include { GATK4_MERGEVCFS as MERGE_FREEBAYES         } from '../../../modules/nf-core/gatk4/mergevcfs'
-include { TABIX_TABIX     as TABIX_VC_FREEBAYES      } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX     as TABIX_VC_FREEBAYES_FILT } from '../../../modules/nf-core/tabix/tabix'
+include { HTSLIB_BGZIPTABIX as TABIX_VC_FREEBAYES      } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_VC_FREEBAYES_FILT } from '../../../modules/nf-core/htslib/bgziptabix'
 include { VCFLIB_VCFFILTER                           } from '../../../modules/nf-core/vcflib/vcffilter'
 
 workflow BAM_VARIANT_CALLING_FREEBAYES {
@@ -20,8 +20,6 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     ch_intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
-
     // Combine cram and intervals for spread and gather strategy
     cram_intervals = ch_cram.combine(ch_intervals)
         // Move num_intervals to meta map and reorganize channel for FREEBAYES module
@@ -43,7 +41,7 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     MERGE_FREEBAYES(vcf_to_merge, ch_dict)
 
     // Only when no_intervals
-    TABIX_VC_FREEBAYES(bcftools_vcf_out.no_intervals)
+    TABIX_VC_FREEBAYES(bcftools_vcf_out.no_intervals.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, '')
 
     // Mix intervals and no_intervals channels together, including the tabix index
     merged_vcf_with_tbi = MERGE_FREEBAYES.out.vcf
@@ -51,7 +49,7 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
         .map{ meta, vcf, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'freebayes' ], vcf, tbi ] }
 
     no_intervals_with_tbi = bcftools_vcf_out.no_intervals
-        .join(TABIX_VC_FREEBAYES.out.tbi, by: [0])
+        .join(TABIX_VC_FREEBAYES.out.index, by: [0])
         .map{ meta, vcf, tbi -> [ meta - meta.subMap('num_intervals') + [ variantcaller:'freebayes' ], vcf, tbi ] }
 
     // Final channel with VCF and its index
@@ -62,16 +60,12 @@ workflow BAM_VARIANT_CALLING_FREEBAYES {
     vcf_filtered = VCFLIB_VCFFILTER.out.vcf
 
     // Index the filtered VCFs
-    TABIX_VC_FREEBAYES_FILT(vcf_filtered)
-
-    versions = versions.mix(TABIX_VC_FREEBAYES.out.versions)
-    versions = versions.mix(TABIX_VC_FREEBAYES_FILT.out.versions)
+    TABIX_VC_FREEBAYES_FILT(vcf_filtered.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, '')
 
     emit:
     vcf_unfiltered = ch_vcf // channel: [ meta, vcf, tbi ]
 
     // Use the QUAL filtered vcfs for the next steps
     vcf = vcf_filtered                         // channel: [ meta, vcf ]
-    tbi = TABIX_VC_FREEBAYES_FILT.out.tbi      // channel: [ meta, tbi ]
-    versions
+    tbi = TABIX_VC_FREEBAYES_FILT.out.index    // channel: [ meta, tbi ]
 }
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index 1a1c7af1fc..abea346137 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -104,7 +104,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             intervals_bed_combined.map{_intervals -> _intervals ? [[id:_intervals[0].baseName], _intervals]: [[id:'no_intervals'], []]},
             params.cnvkit_reference ? cnvkit_reference.map{ reference -> [[id:reference[0].baseName], reference] } : [[:],[]]
         )
-        versions = versions.mix(BAM_VARIANT_CALLING_CNVKIT.out.versions)
     }
 
     // DEEPVARIANT
@@ -135,7 +134,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
         vcf_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.vcf
         tbi_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_FREEBAYES.out.versions)
     }
 
     // HAPLOTYPECALLER
@@ -231,8 +229,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             sentieon_dnascope_pcr_indel_model,
             sentieon_dnascope_model)
 
-        versions = versions.mix(BAM_VARIANT_CALLING_SENTIEON_DNASCOPE.out.versions)
-
         vcf_sentieon_dnascope      = BAM_VARIANT_CALLING_SENTIEON_DNASCOPE.out.vcf
         tbi_sentieon_dnascope      = BAM_VARIANT_CALLING_SENTIEON_DNASCOPE.out.vcf_tbi
         gvcf_sentieon_dnascope     = BAM_VARIANT_CALLING_SENTIEON_DNASCOPE.out.gvcf
@@ -257,7 +253,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
             vcf_sentieon_dnascope = BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.genotype_vcf
             tbi_sentieon_dnascope = BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.genotype_index
-            versions = versions.mix(BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.versions)
         } else {
             // If single sample track, check if filtering should be done
             if (!(skip_tools && skip_tools.split(',').contains('dnascope_filter'))) {
@@ -288,8 +283,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
             joint_germline,
             sentieon_haplotyper_emit_mode)
 
-        versions = versions.mix(BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER.out.versions)
-
         vcf_sentieon_haplotyper      = BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER.out.vcf
         tbi_sentieon_haplotyper      = BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER.out.vcf_tbi
         gvcf_sentieon_haplotyper     = BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER.out.gvcf
@@ -314,7 +307,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
             vcf_sentieon_haplotyper = BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.genotype_vcf
             tbi_sentieon_haplotyper = BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.genotype_index
-            versions = versions.mix(BAM_JOINT_CALLING_GERMLINE_SENTIEON.out.versions)
         } else {
 
             // If single sample track, check if filtering should be done
diff --git a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
index 5c4fd80957..8e5901f198 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_dnascope/main.nf
@@ -23,8 +23,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
     sentieon_dnascope_model           // channel
 
     main:
-    versions = channel.empty()
-
     gvcf               = channel.empty()
     vcf                = channel.empty()
     genotype_intervals = channel.empty()
@@ -143,7 +141,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_DNASCOPE {
 
 
     emit:
-    versions
     vcf
     vcf_tbi
     gvcf
diff --git a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
index f014af1740..6fea625118 100644
--- a/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
+++ b/subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
@@ -21,8 +21,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
     sentieon_haplotyper_emit_mode
 
     main:
-    versions = channel.empty()
-
     gvcf               = channel.empty()
     vcf                = channel.empty()
     genotype_intervals = channel.empty()
@@ -140,7 +138,6 @@ workflow BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER {
 
 
     emit:
-    versions
     vcf
     vcf_tbi
     gvcf
diff --git a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
index 001fabc257..ef5afcb7ab 100644
--- a/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
+++ b/subworkflows/local/bam_variant_calling_single_tiddit/main.nf
@@ -4,7 +4,7 @@
 // For all modules here:
 // A when clause condition is defined in the conf/modules.config to determine if the module should be run
 
-include { TABIX_BGZIPTABIX as TABIX_BGZIP_TIDDIT_SV } from '../../../modules/nf-core/tabix/bgziptabix/main'
+include { HTSLIB_BGZIPTABIX as TABIX_BGZIP_TIDDIT_SV } from '../../../modules/nf-core/htslib/bgziptabix/main'
 include { TIDDIT_SV                                 } from '../../../modules/nf-core/tiddit/sv/main'
 
 workflow BAM_VARIANT_CALLING_SINGLE_TIDDIT {
@@ -18,11 +18,11 @@ workflow BAM_VARIANT_CALLING_SINGLE_TIDDIT {
 
     TIDDIT_SV(cram, fasta.combine(fasta_fai).map { fasta_meta, fasta_path, _fai_meta, fai_path -> [ fasta_meta, fasta_path, fai_path ] }, bwa)
 
-    TABIX_BGZIP_TIDDIT_SV(TIDDIT_SV.out.vcf)
+    TABIX_BGZIP_TIDDIT_SV(TIDDIT_SV.out.vcf.map { meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, 'vcf')
 
     ploidy = TIDDIT_SV.out.ploidy
-    vcf = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, gz, _tbi -> [meta + [variantcaller: 'tiddit'], gz] }
-    tbi = TABIX_BGZIP_TIDDIT_SV.out.gz_index.map { meta, _gz, tbi -> [meta + [variantcaller: 'tiddit'], tbi] }
+    vcf = TABIX_BGZIP_TIDDIT_SV.out.output.join(TABIX_BGZIP_TIDDIT_SV.out.index).map { meta, gz, _tbi -> [meta + [variantcaller: 'tiddit'], gz] }
+    tbi = TABIX_BGZIP_TIDDIT_SV.out.output.join(TABIX_BGZIP_TIDDIT_SV.out.index).map { meta, _gz, tbi -> [meta + [variantcaller: 'tiddit'], tbi] }
 
     emit:
     ploidy
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index 2c43feee40..5d9a0210f7 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -132,8 +132,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
             intervals_bed_combined.map { _intervals -> _intervals ? [[id: _intervals[0].baseName], _intervals] : [[id: 'no_intervals'], []] },
             [[id: "null"], []],
         )
-
-        versions = versions.mix(BAM_VARIANT_CALLING_CNVKIT.out.versions)
     }
 
     // FREEBAYES
@@ -148,7 +146,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
 
         vcf_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.vcf
         tbi_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_FREEBAYES.out.versions)
     }
 
     // MANTA
@@ -262,7 +259,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
 
         vcf_tnscope = BAM_VARIANT_CALLING_SOMATIC_TNSCOPE.out.vcf
         tbi_tnscope = BAM_VARIANT_CALLING_SOMATIC_TNSCOPE.out.index
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_TNSCOPE.out.versions)
     }
 
     // TIDDIT
diff --git a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
index c66b1b9148..ea40562a63 100644
--- a/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_tnscope/main.nf
@@ -19,8 +19,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TNSCOPE {
     intervals                 // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
-
     // Combine input and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
         // Move num_intervals to meta map and reorganize channel for TNSCOPE module
@@ -68,5 +66,4 @@ workflow BAM_VARIANT_CALLING_SOMATIC_TNSCOPE {
     emit:
     vcf      // channel: [ meta, vcf ]
     index    // channel: [ meta, index ]
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
index b72ffb0fea..8a0b92ec41 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
@@ -43,9 +43,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
     main:
     // Channels are often remapped to match module/subworkflow
 
-    // Gather all versions
-    versions = channel.empty()
-
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
     out_msisensor2 = channel.empty()
     vcf_freebayes  = channel.empty()
@@ -102,8 +99,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
             [[id: "null"], []],
             cnvkit_reference.map { reference -> [[id: reference[0].baseName], reference] },
         )
-
-        versions = versions.mix(BAM_VARIANT_CALLING_CNVKIT.out.versions)
     }
 
     // FREEBAYES
@@ -118,7 +113,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
 
         vcf_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.vcf
         tbi_freebayes = BAM_VARIANT_CALLING_FREEBAYES.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_FREEBAYES.out.versions)
     }
 
     // MSISENSOR
@@ -209,7 +203,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
 
         vcf_tnscope = BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE.out.vcf
         tbi_tnscope = BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE.out.tbi
-        versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE.out.versions)
     }
 
     vcf_all = channel.empty()
@@ -252,5 +245,4 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_ALL {
     tbi_mutect2
     tbi_tiddit
     tbi_tnscope
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
index 0c233bad0e..9a8e69fece 100644
--- a/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
+++ b/subworkflows/local/bam_variant_calling_tumor_only_tnscope/main.nf
@@ -19,8 +19,6 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE {
     intervals                 // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
 
     main:
-    versions = channel.empty()
-
     // Combine input and intervals for spread and gather strategy
     input_intervals = input.combine(intervals)
         // Move num_intervals to meta map and reorganize channel for TNSCOPE module
@@ -69,5 +67,4 @@ workflow BAM_VARIANT_CALLING_TUMOR_ONLY_TNSCOPE {
     vcf      // channel: [ meta, vcf ]
     tbi = index // channel: [ meta, tbi ]
     index    // channel: [ meta, index ]
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/post_variantcalling/main.nf b/subworkflows/local/post_variantcalling/main.nf
index 277bed9765..1dbfd3059c 100644
--- a/subworkflows/local/post_variantcalling/main.nf
+++ b/subworkflows/local/post_variantcalling/main.nf
@@ -56,7 +56,6 @@ workflow POST_VARIANTCALLING {
 
         vcfs = vcfs.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.vcf)
         tbis = tbis.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.tbi)
-        versions = versions.mix(VCF_VARLOCIRAPTOR_SOMATIC.out.versions)
 
         // TUMOR ONLY
         VCF_VARLOCIRAPTOR_TUMOR_ONLY(cram_tumor_only, fasta, fai, varlociraptor_scenario_tumor_only, tumor_only_vcfs, varlociraptor_chunk_size, 'tumor', varlociraptor_events_tumor_only, varlociraptor_fdr)
@@ -147,8 +146,6 @@ workflow POST_VARIANTCALLING {
             // Mix consensus VCF with individual caller VCFs for downstream annotation
             small_variant_vcfs = consensus_vcfs.mix(individual_caller_vcfs)
             small_variant_tbis = consensus_tbis.mix(individual_caller_tbis)
-
-            versions = versions.mix(CONSENSUS.out.versions)
         }
 
         vcfs = small_variant_vcfs.mix(all_vcfs.other)
diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf
index 9856373a6c..7534e3bf24 100644
--- a/subworkflows/local/prepare_genome/main.nf
+++ b/subworkflows/local/prepare_genome/main.nf
@@ -5,12 +5,12 @@ include { DRAGMAP_HASHTABLE                         } from '../../../modules/nf-
 include { GATK4_CREATESEQUENCEDICTIONARY            } from '../../../modules/nf-core/gatk4/createsequencedictionary'
 include { MSISENSORPRO_SCAN                         } from '../../../modules/nf-core/msisensorpro/scan'
 include { SAMTOOLS_FAIDX                            } from '../../../modules/nf-core/samtools/faidx'
-include { TABIX_TABIX as TABIX_BCFTOOLS_ANNOTATIONS } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_DBSNP                } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_GERMLINE_RESOURCE    } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_KNOWN_INDELS         } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_KNOWN_SNPS           } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_PON                  } from '../../../modules/nf-core/tabix/tabix'
+include { HTSLIB_BGZIPTABIX as TABIX_BCFTOOLS_ANNOTATIONS } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_DBSNP                } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_GERMLINE_RESOURCE    } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_KNOWN_INDELS         } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_KNOWN_SNPS           } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_PON                  } from '../../../modules/nf-core/htslib/bgziptabix'
 include { UNTAR as UNTAR_BBSPLIT_INDEX              } from '../../../modules/nf-core/untar'
 include { UNTAR as UNTAR_CHR_DIR                    } from '../../../modules/nf-core/untar'
 include { UNTAR as UNTAR_MSISENSOR2_MODELS          } from '../../../modules/nf-core/untar'
@@ -54,8 +54,6 @@ workflow PREPARE_GENOME {
     vep_include_fasta           // params.vep_include_fasta
 
     main:
-    versions = channel.empty()
-
     // TODO: EXTRACT FASTA FILE?
     fasta = fasta_in ? channel.fromPath(fasta_in).map { fasta -> [[id: fasta.baseName], fasta] }.collect() : channel.empty()
     vep_fasta = vep_include_fasta ? fasta : [[id: 'null'], []]
@@ -144,8 +142,8 @@ workflow PREPARE_GENOME {
     bcftools_annotations_tbi = bcftools_annotations_tbi_in ? channel.fromPath(bcftools_annotations_tbi_in).collect() : channel.value([])
 
     if (!bcftools_annotations_tbi_in && bcftools_annotations_in) {
-        TABIX_BCFTOOLS_ANNOTATIONS(bcftools_annotations.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        bcftools_annotations_tbi = TABIX_BCFTOOLS_ANNOTATIONS.out.tbi.map { _meta, tbi -> [tbi] }.collect()
+        TABIX_BCFTOOLS_ANNOTATIONS(bcftools_annotations.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        bcftools_annotations_tbi = TABIX_BCFTOOLS_ANNOTATIONS.out.index.map { _meta, tbi -> [tbi] }.collect()
 
     }
 
@@ -153,45 +151,40 @@ workflow PREPARE_GENOME {
     dbsnp_tbi = dbsnp_tbi_in ? channel.fromPath(dbsnp_tbi_in).collect() : channel.value([])
 
     if (!dbsnp_tbi_in && dbsnp_in && ((step == "mapping" || step == "markduplicates" || step == "prepare_recalibration") || (tools.split(',').contains('controlfreec') || tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope') || tools.split(',').contains('muse') || tools.split(',').contains('mutect2')))) {
-        TABIX_DBSNP(dbsnp.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        dbsnp_tbi = TABIX_DBSNP.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_DBSNP.out.versions)
+        TABIX_DBSNP(dbsnp.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        dbsnp_tbi = TABIX_DBSNP.out.index.map { _meta, tbi -> [tbi] }.collect()
     }
 
     germline_resource = germline_resource_in ? channel.fromPath(germline_resource_in).collect() : channel.value([])
     germline_resource_tbi = germline_resource_tbi_in ? channel.fromPath(germline_resource_tbi_in).collect() : channel.value([])
 
     if (!germline_resource_tbi_in && germline_resource_in && (tools.split(',').contains('mutect2') || tools.split(',').contains('sentieon_tnscope'))) {
-        TABIX_GERMLINE_RESOURCE(germline_resource.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        germline_resource_tbi = TABIX_GERMLINE_RESOURCE.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_GERMLINE_RESOURCE.out.versions)
+        TABIX_GERMLINE_RESOURCE(germline_resource.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        germline_resource_tbi = TABIX_GERMLINE_RESOURCE.out.index.map { _meta, tbi -> [tbi] }.collect()
     }
 
     known_indels = known_indels_in ? channel.fromPath(known_indels_in).collect() : channel.value([])
     known_indels_tbi = known_indels_tbi_in ? channel.fromPath(known_indels_tbi_in).collect() : channel.value([])
 
     if (!known_indels_tbi_in && known_indels_in && (step == 'mapping' || step == "markduplicates" || step == 'prepare_recalibration' || (tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope')))) {
-        TABIX_KNOWN_INDELS(known_indels.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        known_indels_tbi = TABIX_KNOWN_INDELS.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_KNOWN_INDELS.out.versions)
+        TABIX_KNOWN_INDELS(known_indels.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        known_indels_tbi = TABIX_KNOWN_INDELS.out.index.map { _meta, tbi -> [tbi] }.collect()
     }
 
     known_snps = known_snps_in ? channel.fromPath(known_snps_in).collect() : channel.value([])
     known_snps_tbi = known_snps_tbi_in ? channel.fromPath(known_snps_tbi_in).collect() : channel.value([])
 
     if (!known_snps_tbi_in && known_snps_in && (step == 'mapping' || step == "markduplicates" || step == 'prepare_recalibration' || (tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper')))) {
-        TABIX_KNOWN_SNPS(known_snps.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        known_snps_tbi = TABIX_KNOWN_SNPS.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_KNOWN_SNPS.out.versions)
+        TABIX_KNOWN_SNPS(known_snps.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        known_snps_tbi = TABIX_KNOWN_SNPS.out.index.map { _meta, tbi -> [tbi] }.collect()
     }
 
     pon = pon_in ? channel.fromPath(pon_in).collect() : channel.value([])
     pon_tbi = pon_tbi_in ? channel.fromPath(pon_tbi_in).collect() : channel.value([])
 
     if (!pon_tbi_in && pon_in && tools.split(',').contains('mutect2')) {
-        TABIX_PON(pon.flatten().map { vcf -> [[id: vcf.baseName], vcf] })
-        pon_tbi = TABIX_PON.out.tbi.map { _meta, tbi -> [tbi] }.collect()
-        versions = versions.mix(TABIX_PON.out.versions)
+        TABIX_PON(pon.flatten().map { vcf -> [[id: vcf.baseName], vcf, [], []] }, 'compress', true, '')
+        pon_tbi = TABIX_PON.out.index.map { _meta, tbi -> [tbi] }.collect()
     }
 
     // known_sites is made by grouping both the dbsnp and the known snps/indels resources
@@ -310,5 +303,4 @@ workflow PREPARE_GENOME {
     pon                      // Channel: [pon]
     pon_tbi                  // Channel: [pon_tbi]
     vep_fasta                // Channel: [meta, vep_fasta]
-    versions                 // Channel: [versions.yml]
 }
diff --git a/subworkflows/local/prepare_genome/tests/bbsplit.nf.test b/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
index 026ed1dd9c..34ca3976e8 100644
--- a/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
+++ b/subworkflows/local/prepare_genome/tests/bbsplit.nf.test
@@ -58,8 +58,7 @@ nextflow_workflow {
             assertAll(
                 { assert workflow.success},
                 { assert snapshot(
-                    workflow.out.bbsplit_index,
-                    workflow.out.versions
+                    workflow.out.bbsplit_index
                 ).match() }
             )
         }
@@ -171,8 +170,7 @@ nextflow_workflow {
             assertAll(
                 { assert workflow.success },
                 { assert snapshot(
-                    workflow.out.bbsplit_index,
-                    workflow.out.versions
+                    workflow.out.bbsplit_index
                 ).match() }
             )
         }
@@ -230,8 +228,7 @@ nextflow_workflow {
             assertAll(
                 { assert workflow.success },
                 { assert snapshot(
-                    workflow.out.bbsplit_index,
-                    workflow.out.versions
+                    workflow.out.bbsplit_index
                 ).match() }
             )
         }
@@ -292,8 +289,7 @@ nextflow_workflow {
             assertAll(
                 { assert workflow.success },
                 { assert snapshot(
-                    workflow.out.bbsplit_index,
-                    workflow.out.versions
+                    workflow.out.bbsplit_index
                 ).match() }
             )
         }
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index 55bf2e233a..d51d2b2728 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -9,8 +9,8 @@
 include { CREATE_INTERVALS_BED                                   } from '../../../modules/local/create_intervals_bed'
 include { GATK4_INTERVALLISTTOBED                                } from '../../../modules/nf-core/gatk4/intervallisttobed'
 include { GAWK as BUILD_INTERVALS                                } from '../../../modules/nf-core/gawk'
-include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_INTERVAL_SPLIT    } from '../../../modules/nf-core/tabix/bgziptabix'
-include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_INTERVAL_COMBINED } from '../../../modules/nf-core/tabix/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_BGZIPTABIX_INTERVAL_SPLIT    } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_BGZIPTABIX_INTERVAL_COMBINED } from '../../../modules/nf-core/htslib/bgziptabix'
 
 workflow PREPARE_INTERVALS {
     take:
@@ -29,7 +29,7 @@ workflow PREPARE_INTERVALS {
     intervals_combined   = channel.empty() // Single bed file containing all intervals
 
     if (no_intervals) {
-        file("${outdir}/no_intervals.bed").text        = "no_intervals\n"
+        file("${outdir}/no_intervals.bed").text        = ""
         file("${outdir}/no_intervals.bed.gz").text     = "no_intervals\n"
         file("${outdir}/no_intervals.bed.gz.tbi").text = "no_intervals\n"
 
@@ -88,18 +88,18 @@ workflow PREPARE_INTERVALS {
             .transpose()
 
         // 2. Create bed.gz and bed.gz.tbi for each interval file. They are split by region (see above)
-        TABIX_BGZIPTABIX_INTERVAL_SPLIT(intervals_bed.map{ file, _num_intervals -> [ [ id:file.baseName], file ] })
+        TABIX_BGZIPTABIX_INTERVAL_SPLIT(intervals_bed.map{ file, _num_intervals -> [ [ id:file.baseName], file, [], [] ] }, 'compress', true, 'bed')
 
-        intervals_bed_gz_tbi = TABIX_BGZIPTABIX_INTERVAL_SPLIT.out.gz_index.map{ _meta, bed, tbi -> [ bed, tbi ] }.toList()
+        intervals_bed_gz_tbi = TABIX_BGZIPTABIX_INTERVAL_SPLIT.out.output.join(TABIX_BGZIPTABIX_INTERVAL_SPLIT.out.index).map{ _meta, bed, tbi -> [ bed, tbi ] }.toList()
             // Adding number of intervals as elements
             .map{ files -> [ files, files.size() ] }
             .transpose()
     }
 
-    TABIX_BGZIPTABIX_INTERVAL_COMBINED(intervals_combined)
+    TABIX_BGZIPTABIX_INTERVAL_COMBINED(intervals_combined.map{ meta, bed -> [ meta, bed, [], [] ] }, 'compress', true, 'bed')
 
     intervals_bed_combined        = intervals_combined.map{_meta, bed -> bed }.collect()
-    intervals_bed_gz_tbi_combined = TABIX_BGZIPTABIX_INTERVAL_COMBINED.out.gz_index.map{_meta, gz, tbi -> [gz, tbi] }.collect()
+    intervals_bed_gz_tbi_combined = TABIX_BGZIPTABIX_INTERVAL_COMBINED.out.output.join(TABIX_BGZIPTABIX_INTERVAL_COMBINED.out.index).map{_meta, gz, tbi -> [gz, tbi] }.collect()
 
     emit:
     // Intervals split for parallel execution
diff --git a/subworkflows/local/prepare_reference_cnvkit/main.nf b/subworkflows/local/prepare_reference_cnvkit/main.nf
index 74b8b3694c..cf5b97b01f 100644
--- a/subworkflows/local/prepare_reference_cnvkit/main.nf
+++ b/subworkflows/local/prepare_reference_cnvkit/main.nf
@@ -7,16 +7,10 @@ workflow PREPARE_REFERENCE_CNVKIT {
     intervals_bed_combined // channel: []
 
     main:
-    versions = channel.empty()
-
     // prepare a antitarget reference files for tumor_only mode of cnvkit
     CNVKIT_ANTITARGET(intervals_bed_combined.flatten().map { bed -> [[id: 'intervals'], bed] })
     CNVKIT_REFERENCE(fasta.map { _meta, fasta_ -> [fasta_] }, intervals_bed_combined, CNVKIT_ANTITARGET.out.bed.map { _meta, bed -> [bed] })
 
-    versions = versions.mix(CNVKIT_ANTITARGET.out.versions)
-    versions = versions.mix(CNVKIT_REFERENCE.out.versions)
-
     emit:
     cnvkit_reference = CNVKIT_REFERENCE.out.cnn.collect()
-    versions
 }
diff --git a/subworkflows/local/vcf_annotate_all/main.nf b/subworkflows/local/vcf_annotate_all/main.nf
index ea559ed6c0..e274499efd 100644
--- a/subworkflows/local/vcf_annotate_all/main.nf
+++ b/subworkflows/local/vcf_annotate_all/main.nf
@@ -30,7 +30,6 @@ workflow VCF_ANNOTATE_ALL {
     vcf_ann = channel.empty()
     tab_ann = channel.empty()
     json_ann = channel.empty()
-    versions = channel.empty()
 
     if (tools.split(',').contains('bcfann')) {
         BCFTOOLS_ANNOTATE(
@@ -78,5 +77,4 @@ workflow VCF_ANNOTATE_ALL {
     vcf_ann  // channel: [ val(meta), vcf.gz, vcf.gz.tbi ]
     tab_ann
     json_ann
-    versions //    path: versions.yml
 }
diff --git a/subworkflows/local/vcf_concatenate_germline/main.nf b/subworkflows/local/vcf_concatenate_germline/main.nf
index 3c3010666f..db649fdaab 100644
--- a/subworkflows/local/vcf_concatenate_germline/main.nf
+++ b/subworkflows/local/vcf_concatenate_germline/main.nf
@@ -6,8 +6,7 @@
 include { ADD_INFO_TO_VCF                                } from '../../../modules/local/add_info_to_vcf'
 include { BCFTOOLS_CONCAT as GERMLINE_VCFS_CONCAT        } from '../../../modules/nf-core/bcftools/concat'
 include { BCFTOOLS_SORT as GERMLINE_VCFS_CONCAT_SORT     } from '../../../modules/nf-core/bcftools/sort'
-include { TABIX_BGZIPTABIX as TABIX_EXT_VCF              } from '../../../modules/nf-core/tabix/bgziptabix'
-include { TABIX_TABIX as TABIX_GERMLINE_VCFS_CONCAT_SORT } from '../../../modules/nf-core/tabix/tabix'
+include { HTSLIB_BGZIPTABIX as TABIX_EXT_VCF             } from '../../../modules/nf-core/htslib/bgziptabix'
 
 workflow CONCATENATE_GERMLINE_VCFS {
     take:
@@ -18,10 +17,10 @@ workflow CONCATENATE_GERMLINE_VCFS {
 
     // Concatenate vcf-files
     ADD_INFO_TO_VCF(vcfs)
-    TABIX_EXT_VCF(ADD_INFO_TO_VCF.out.vcf)
+    TABIX_EXT_VCF(ADD_INFO_TO_VCF.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, 'vcf')
 
     // Gather vcfs and vcf-tbis for concatenating germline-vcfs
-    germline_vcfs_with_tbis = TABIX_EXT_VCF.out.gz_index.groupTuple()
+    germline_vcfs_with_tbis = TABIX_EXT_VCF.out.output.join(TABIX_EXT_VCF.out.index).groupTuple()
 
     GERMLINE_VCFS_CONCAT(germline_vcfs_with_tbis)
     GERMLINE_VCFS_CONCAT_SORT(GERMLINE_VCFS_CONCAT.out.vcf)
diff --git a/subworkflows/local/vcf_consensus/main.nf b/subworkflows/local/vcf_consensus/main.nf
index 8cedb412b2..933c692115 100644
--- a/subworkflows/local/vcf_consensus/main.nf
+++ b/subworkflows/local/vcf_consensus/main.nf
@@ -12,8 +12,6 @@ workflow CONSENSUS {
     vcfs     // [meta, vcf ,tbi]
 
     main:
-    ch_versions = channel.empty()
-
     ch_vcfs = vcfs
         .branch{ meta, _vcf, _tbi ->
             // Somatic Strelka samples have tumor_id field (tumor-normal pairs)
@@ -68,11 +66,9 @@ workflow CONSENSUS {
         }
 
     // Create consensus VCF from sites.txt with caller presence info
-    // Versions are collected via topic channel
     CONSENSUS_FROM_SITES(ch_isec_with_results)
 
     emit:
-    versions = ch_versions
     vcfs     = CONSENSUS_FROM_SITES.out.vcf
     tbis     = CONSENSUS_FROM_SITES.out.tbi
 
diff --git a/subworkflows/local/vcf_normalization/main.nf b/subworkflows/local/vcf_normalization/main.nf
index 8878eeacd3..4c17cbe412 100644
--- a/subworkflows/local/vcf_normalization/main.nf
+++ b/subworkflows/local/vcf_normalization/main.nf
@@ -4,7 +4,7 @@
 include { ADD_INFO_TO_VCF                   } from '../../../modules/local/add_info_to_vcf'
 include { BCFTOOLS_NORM as VCFS_NORM        } from '../../../modules/nf-core/bcftools/norm'
 include { BCFTOOLS_SORT as VCFS_NORM_SORT   } from '../../../modules/nf-core/bcftools/sort'
-include { TABIX_BGZIPTABIX as TABIX_EXT_VCF } from '../../../modules/nf-core/tabix/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_EXT_VCF } from '../../../modules/nf-core/htslib/bgziptabix'
 
 // Workflow to normalize, compress, and index VCF files
 workflow NORMALIZE_VCFS {
@@ -19,10 +19,10 @@ workflow NORMALIZE_VCFS {
     ADD_INFO_TO_VCF(vcfs)
 
     // Compress the VCF files with bgzip
-    TABIX_EXT_VCF(ADD_INFO_TO_VCF.out.vcf)
+    TABIX_EXT_VCF(ADD_INFO_TO_VCF.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, 'vcf')
 
     // Normalize the VCF files with BCFTOOLS_NORM
-    VCFS_NORM(TABIX_EXT_VCF.out.gz_index, fasta)
+    VCFS_NORM(TABIX_EXT_VCF.out.output.join(TABIX_EXT_VCF.out.index), fasta)
 
     // Sort the normalized VCF files
     VCFS_NORM_SORT(VCFS_NORM.out.vcf)
diff --git a/subworkflows/local/vcf_varlociraptor_somatic/main.nf b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
index 7234a5d072..b43ff4dfa2 100644
--- a/subworkflows/local/vcf_varlociraptor_somatic/main.nf
+++ b/subworkflows/local/vcf_varlociraptor_somatic/main.nf
@@ -3,8 +3,8 @@ include { BCFTOOLS_CONCAT as CONCAT_SOMATIC_STRELKA
 include { BCFTOOLS_MERGE as MERGE_GERMLINE_SOMATIC_VCFS                           } from '../../../modules/nf-core/bcftools/merge'
 include { BCFTOOLS_SORT as SORT_CALLED_CHUNKS                                     } from '../../../modules/nf-core/bcftools/sort'
 include { BCFTOOLS_SORT as SORT_FINAL_VCF                                         } from '../../../modules/nf-core/bcftools/sort'
-include { TABIX_TABIX as TABIX_GERMLINE                                           } from '../../../modules/nf-core/tabix/tabix'
-include { TABIX_TABIX as TABIX_SOMATIC                                            } from '../../../modules/nf-core/tabix/tabix'
+include { HTSLIB_BGZIPTABIX as TABIX_GERMLINE                                     } from '../../../modules/nf-core/htslib/bgziptabix'
+include { HTSLIB_BGZIPTABIX as TABIX_SOMATIC                                      } from '../../../modules/nf-core/htslib/bgziptabix'
 include { RBT_VCFSPLIT                                                            } from '../../../modules/nf-core/rbt/vcfsplit'
 include { VARLOCIRAPTOR_CALLVARIANTS                                              } from '../../../modules/nf-core/varlociraptor/callvariants'
 include { VARLOCIRAPTOR_ESTIMATEALIGNMENTPROPERTIES as ALIGNMENTPROPERTIES_NORMAL } from '../../../modules/nf-core/varlociraptor/estimatealignmentproperties'
@@ -27,7 +27,6 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     val_fdr
 
     main:
-    ch_versions = channel.empty()
 
     meta_map = ch_cram.map { meta, _normal_cram, _normal_crai, _tumor_cram, _tumor_crai ->
         meta + [sex_string: (meta.sex == "XX" ? "female" : "male")]
@@ -57,9 +56,8 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     //
     // CONCAT SNV AND INDEL VCFS FOR STRELKA
     //
-    TABIX_SOMATIC(ch_somatic_vcf)
-    ch_versions = ch_versions.mix(TABIX_SOMATIC.out.versions)
-    ch_somatic_vcf_tbi = ch_somatic_vcf.join(TABIX_SOMATIC.out.tbi, by: [0])
+    TABIX_SOMATIC(ch_somatic_vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, '')
+    ch_somatic_vcf_tbi = ch_somatic_vcf.join(TABIX_SOMATIC.out.index, by: [0])
 
     // CONCAT SNV / INDEL VCFs COMING FROM STRELKA
     ch_somatic_branched = ch_somatic_vcf_tbi.branch { meta, _vcf, _tbi ->
@@ -85,9 +83,8 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     //
     // MERGE GERMLINE AND SOMATIC VCFs
     //
-    TABIX_GERMLINE(ch_germline_vcf)
-    ch_versions = ch_versions.mix(TABIX_GERMLINE.out.versions)
-    ch_germline_vcf_tbi = ch_germline_vcf.join(TABIX_GERMLINE.out.tbi, by: [0])
+    TABIX_GERMLINE(ch_germline_vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, '')
+    ch_germline_vcf_tbi = ch_germline_vcf.join(TABIX_GERMLINE.out.index, by: [0])
 
     def somatic_with_key = ch_somatic_vcf_conc.map { meta, vcf, tbi ->
         [[id: meta.normal_id, variantcaller: meta.variantcaller], meta, vcf, tbi]
@@ -288,5 +285,4 @@ workflow VCF_VARLOCIRAPTOR_SOMATIC {
     emit:
     vcf      = SORT_FINAL_VCF.out.vcf
     tbi      = SORT_FINAL_VCF.out.index
-    versions = ch_versions
 }
diff --git a/subworkflows/nf-core/vcf_annotate_snpeff/main.nf b/subworkflows/nf-core/vcf_annotate_snpeff/main.nf
index 3eccc896f8..3ffe0ebf50 100644
--- a/subworkflows/nf-core/vcf_annotate_snpeff/main.nf
+++ b/subworkflows/nf-core/vcf_annotate_snpeff/main.nf
@@ -3,7 +3,7 @@
 //
 
 include { SNPEFF_SNPEFF    } from '../../../modules/nf-core/snpeff/snpeff'
-include { TABIX_BGZIPTABIX } from '../../../modules/nf-core/tabix/bgziptabix'
+include { HTSLIB_BGZIPTABIX } from '../../../modules/nf-core/htslib/bgziptabix'
 
 workflow VCF_ANNOTATE_SNPEFF {
     take:
@@ -13,12 +13,20 @@ workflow VCF_ANNOTATE_SNPEFF {
 
     main:
     SNPEFF_SNPEFF(ch_vcf, val_snpeff_db, ch_snpeff_cache)
-    TABIX_BGZIPTABIX(SNPEFF_SNPEFF.out.vcf)
+    HTSLIB_BGZIPTABIX(
+        SNPEFF_SNPEFF.out.vcf.map { meta, vcf -> [ meta, vcf, [], [] ] },
+        "compress",
+        true,
+        "vcf"
+    )
 
+    ch_vcf_tbi = HTSLIB_BGZIPTABIX.out.output.join(
+        HTSLIB_BGZIPTABIX.out.index
+    )
 
     emit:
-    vcf_tbi   = TABIX_BGZIPTABIX.out.gz_index // channel: [ val(meta), path(vcf), path(tbi) ]
-    reports   = SNPEFF_SNPEFF.out.report // channel: [ path(html) ]
+    vcf_tbi   = ch_vcf_tbi                     // channel: [ val(meta), path(vcf), path(tbi) ]
+    reports   = SNPEFF_SNPEFF.out.report       // channel: [ path(html) ]
     summary   = SNPEFF_SNPEFF.out.summary_html // channel: [ path(html) ]
-    genes_txt = SNPEFF_SNPEFF.out.genes_txt // channel: [ path(genes.txt) ]
+    genes_txt = SNPEFF_SNPEFF.out.genes_txt    // channel: [ path(genes.txt) ]
 }
diff --git a/subworkflows/nf-core/vcf_annotate_snpeff/meta.yml b/subworkflows/nf-core/vcf_annotate_snpeff/meta.yml
index bf2db6523b..17aa43605d 100644
--- a/subworkflows/nf-core/vcf_annotate_snpeff/meta.yml
+++ b/subworkflows/nf-core/vcf_annotate_snpeff/meta.yml
@@ -8,7 +8,7 @@ keywords:
 components:
   - snpeff
   - snpeff/snpeff
-  - tabix/bgziptabix
+  - htslib/bgziptabix
 input:
   - ch_vcf:
       description: |
diff --git a/tests/aligner-bwa-mem.nf.test.snap b/tests/aligner-bwa-mem.nf.test.snap
index f450b48c78..bf60ddd9c9 100644
--- a/tests/aligner-bwa-mem.nf.test.snap
+++ b/tests/aligner-bwa-mem.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -79,11 +79,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:28:32.64734047",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T18:55:05.917351813"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner bwa-mem --save_reference --build_only_index -stub": {
         "content": [
@@ -99,12 +99,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -150,12 +150,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -193,11 +193,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-28T10:56:53.177914755",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.3"
-        },
-        "timestamp": "2025-06-12T10:31:18.476995966"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner bwa-mem --save_reference skip QC/recal/md -stub": {
         "content": [
@@ -226,12 +226,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/aligner-bwa-mem2.nf.test.snap b/tests/aligner-bwa-mem2.nf.test.snap
index 5879c7efa9..50dfd96e4b 100644
--- a/tests/aligner-bwa-mem2.nf.test.snap
+++ b/tests/aligner-bwa-mem2.nf.test.snap
@@ -13,12 +13,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -56,10 +56,10 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2025-06-12T10:35:55.383074123",
+        "timestamp": "2026-07-28T10:58:34.052160784",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.3"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --aligner bwa-mem2 --save_reference skip QC/recal/md": {
@@ -89,12 +89,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -142,10 +142,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2025-12-15T18:58:41.317252772",
+        "timestamp": "2026-07-28T14:29:32.949195758",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --aligner bwa-mem2 --save_reference --build_only_index - stub": {
@@ -162,12 +162,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -226,12 +226,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/aligner-dragmap.nf.test.snap b/tests/aligner-dragmap.nf.test.snap
index 5a1f1fb712..2850663098 100644
--- a/tests/aligner-dragmap.nf.test.snap
+++ b/tests/aligner-dragmap.nf.test.snap
@@ -13,12 +13,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -61,10 +61,10 @@
                 "WARN: DragMap was specified as aligner. Base recalibration is not contained in --skip_tools. It is recommended to skip baserecalibration when using DragMap"
             ]
         ],
-        "timestamp": "2025-12-15T19:02:48.431048619",
+        "timestamp": "2026-07-28T10:58:04.077770914",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --aligner dragmap --save_reference skip QC/recal/md": {
@@ -95,12 +95,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -151,10 +151,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2025-12-15T19:01:59.187593488",
+        "timestamp": "2026-07-28T14:28:21.866075734",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --aligner dragmap --save_reference --build_only_index - stub": {
@@ -171,12 +171,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -232,12 +232,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index 9efe301a64..ff3cb07b10 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -35,12 +35,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -227,7 +227,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T11:40:10.237605568",
+        "timestamp": "2026-07-28T11:02:40.730776056",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -266,12 +266,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -464,7 +464,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T11:40:10.53055388",
+        "timestamp": "2026-07-28T11:04:20.26832985",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -521,12 +521,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -795,7 +795,7 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
-        "timestamp": "2026-07-27T11:45:10.811937172",
+        "timestamp": "2026-07-28T11:09:24.032044733",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -831,12 +831,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -1027,7 +1027,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T11:44:05.461520368",
+        "timestamp": "2026-07-28T11:06:33.397663055",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/alignment_from_everything.nf.test.snap b/tests/alignment_from_everything.nf.test.snap
index 0e37ebd638..71188a93a6 100644
--- a/tests/alignment_from_everything.nf.test.snap
+++ b/tests/alignment_from_everything.nf.test.snap
@@ -73,12 +73,12 @@
                     "spring": "1.1.1"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/alignment_to_fastq.nf.test.snap b/tests/alignment_to_fastq.nf.test.snap
index a2a82b769a..f2698746b9 100644
--- a/tests/alignment_to_fastq.nf.test.snap
+++ b/tests/alignment_to_fastq.nf.test.snap
@@ -64,12 +64,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/annotation_merge.nf.test.snap b/tests/annotation_merge.nf.test.snap
index 1c23b195ce..fe4b81e087 100644
--- a/tests/annotation_merge.nf.test.snap
+++ b/tests/annotation_merge.nf.test.snap
@@ -8,13 +8,13 @@
                     "perl-math-cdf": "0.1",
                     "tabix": "1.23.1"
                 },
+                "HTSLIB_BGZIPTABIX": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                },
                 "SNPEFF_SNPEFF": {
                     "snpeff": "5.4c"
                 },
-                "TABIX_BGZIPTABIX": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
-                },
                 "VCF_ANNOTATE_MERGE": {
                     "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
@@ -109,16 +109,20 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-08T10:14:04.788318141",
+        "timestamp": "2026-07-28T14:26:31.042408461",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.4"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools merge,snpsift": {
         "content": [
             6,
             {
+                "HTSLIB_BGZIPTABIX": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                },
                 "SNPEFF_SNPEFF": {
                     "snpeff": "5.4c"
                 },
@@ -128,10 +132,6 @@
                 "SNPSIFT_ANNMEMCREATE": {
                     "snpsift": "5.4a"
                 },
-                "TABIX_BGZIPTABIX": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
-                },
                 "VCF_ANNOTATE_MERGE": {
                     "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
@@ -220,23 +220,23 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-08T10:15:44.567611717",
+        "timestamp": "2026-07-28T14:26:19.448342505",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.4"
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --tools merge": {
         "content": [
             4,
             {
+                "HTSLIB_BGZIPTABIX": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                },
                 "SNPEFF_SNPEFF": {
                     "snpeff": "5.4c"
                 },
-                "TABIX_BGZIPTABIX": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
-                },
                 "VCF_ANNOTATE_MERGE": {
                     "ensemblvep": "116.0",
                     "perl-math-cdf": "0.1",
@@ -320,10 +320,10 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-08T10:12:53.660022078",
+        "timestamp": "2026-07-28T14:26:11.026803304",
         "meta": {
             "nf-test": "0.9.5",
-            "nextflow": "26.04.4"
+            "nextflow": "25.10.4"
         }
     }
 }
\ No newline at end of file
diff --git a/tests/bbsplit.nf.test.snap b/tests/bbsplit.nf.test.snap
index f214e1b69d..c217a19737 100644
--- a/tests/bbsplit.nf.test.snap
+++ b/tests/bbsplit.nf.test.snap
@@ -52,12 +52,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -422,12 +422,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -647,12 +647,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -838,7 +838,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:25:04.052795149",
+        "timestamp": "2026-07-28T11:03:09.688943886",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/default.nf.test.snap b/tests/default.nf.test.snap
index ab53f6469e..7fb6423efc 100644
--- a/tests/default.nf.test.snap
+++ b/tests/default.nf.test.snap
@@ -46,12 +46,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -379,12 +379,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/fastp.nf.test.snap b/tests/fastp.nf.test.snap
index 9d642ce582..3e32ebea29 100644
--- a/tests/fastp.nf.test.snap
+++ b/tests/fastp.nf.test.snap
@@ -43,12 +43,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -407,12 +407,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -795,12 +795,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/intervals.nf.test.snap b/tests/intervals.nf.test.snap
index 132408824a..96b94ed7c0 100644
--- a/tests/intervals.nf.test.snap
+++ b/tests/intervals.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -77,10 +77,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2025-12-15T19:53:53.927423365",
+        "timestamp": "2026-07-28T14:27:36.729918989",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --intervals genome.multi_intervals.bed --tools null --skip_tools baserecalibrator,fastqc,markduplicates,mosdepth,multiqc,samtools": {
@@ -107,12 +107,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -139,10 +139,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2025-12-15T19:51:26.151477364",
+        "timestamp": "2026-07-28T14:27:26.457705565",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --no_intervals --tools null --skip_tools baserecalibrator,fastqc,markduplicates,mosdepth,multiqc,samtools": {
@@ -166,8 +166,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -197,10 +197,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2025-12-15T19:52:40.407831822",
+        "timestamp": "2026-07-28T14:27:48.805959532",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
         }
     },
     "-profile test --no_intervals --tools null --skip_tools baserecalibrator,fastqc,markduplicates,mosdepth,multiqc,samtools -stub": {
@@ -224,8 +224,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -280,12 +280,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -340,12 +340,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/joint_calling_haplotypecaller.nf.test.snap b/tests/joint_calling_haplotypecaller.nf.test.snap
index d6cf847b4b..df9ffd5934 100644
--- a/tests/joint_calling_haplotypecaller.nf.test.snap
+++ b/tests/joint_calling_haplotypecaller.nf.test.snap
@@ -35,12 +35,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -288,12 +288,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/joint_calling_mutect2.nf.test.snap b/tests/joint_calling_mutect2.nf.test.snap
index 88da7e4892..fc9d9ef0df 100644
--- a/tests/joint_calling_mutect2.nf.test.snap
+++ b/tests/joint_calling_mutect2.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -231,7 +231,7 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
-        "timestamp": "2026-07-27T10:33:51.065452986",
+        "timestamp": "2026-07-28T11:15:22.037134015",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -267,12 +267,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/lane_integer.nf.test.snap b/tests/lane_integer.nf.test.snap
index b2462b19ff..0985055691 100644
--- a/tests/lane_integer.nf.test.snap
+++ b/tests/lane_integer.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -58,10 +58,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T11:00:59.301728305",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T20:43:47.875976457"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/multi_lane.nf.test.snap b/tests/multi_lane.nf.test.snap
index 4a53664d79..b1884c7ebc 100644
--- a/tests/multi_lane.nf.test.snap
+++ b/tests/multi_lane.nf.test.snap
@@ -39,12 +39,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -108,11 +108,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:38:33.629293529",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-06T18:03:04.332516"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/fastq_umi_multi_lane.csv --umi_read_structure '+T 7M1S+T' --skip_tools baserecalibrator,fastqc,markduplicates,mosdepth,multiqc,samtools --tools null": {
         "content": [
@@ -178,12 +178,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -223,7 +223,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_umi_cleanname_1.fastq.gz): Cannot extract flowcell ID from @922332"
             ]
         ],
-        "timestamp": "2026-07-27T10:26:26.437520414",
+        "timestamp": "2026-07-28T11:04:32.774305241",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/postprocess_concatenation.nf.test.snap b/tests/postprocess_concatenation.nf.test.snap
index 6874962f23..2061b3f3e3 100644
--- a/tests/postprocess_concatenation.nf.test.snap
+++ b/tests/postprocess_concatenation.nf.test.snap
@@ -38,22 +38,24 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
diff --git a/tests/postprocess_concatenation_normalization.nf.test.snap b/tests/postprocess_concatenation_normalization.nf.test.snap
index fe3dcb3881..ef58b88808 100644
--- a/tests/postprocess_concatenation_normalization.nf.test.snap
+++ b/tests/postprocess_concatenation_normalization.nf.test.snap
@@ -41,22 +41,24 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -376,22 +378,24 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
diff --git a/tests/postprocess_consensus.nf.test.snap b/tests/postprocess_consensus.nf.test.snap
index dc5a270f5d..0008465822 100644
--- a/tests/postprocess_consensus.nf.test.snap
+++ b/tests/postprocess_consensus.nf.test.snap
@@ -51,16 +51,16 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFS_NORM": {
                     "bcftools": "1.23.1"
@@ -421,16 +421,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFS_NORM": {
                     "bcftools": "1.23.1"
@@ -757,16 +757,16 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFS_NORM": {
                     "bcftools": "1.23.1"
diff --git a/tests/postprocess_filtering.nf.test.snap b/tests/postprocess_filtering.nf.test.snap
index 31b423c532..ada723fa21 100644
--- a/tests/postprocess_filtering.nf.test.snap
+++ b/tests/postprocess_filtering.nf.test.snap
@@ -32,18 +32,20 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
diff --git a/tests/postprocess_normalization.nf.test.snap b/tests/postprocess_normalization.nf.test.snap
index 42f044a65a..9ddd469218 100644
--- a/tests/postprocess_normalization.nf.test.snap
+++ b/tests/postprocess_normalization.nf.test.snap
@@ -32,22 +32,24 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_EXT_VCF": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
diff --git a/tests/postprocess_varlociraptor.nf.test.snap b/tests/postprocess_varlociraptor.nf.test.snap
index 1d9259b618..c3cc403a8b 100644
--- a/tests/postprocess_varlociraptor.nf.test.snap
+++ b/tests/postprocess_varlociraptor.nf.test.snap
@@ -71,18 +71,20 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_GERMLINE": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_SOMATIC": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
                     "varlociraptor": "8.9.5"
@@ -389,12 +391,12 @@
                     "bcftools": "1.23.1"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
                     "varlociraptor": "8.9.5"
@@ -590,7 +592,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:38:20.44515837",
+        "timestamp": "2026-07-28T11:20:47.323833718",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -641,15 +643,16 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_GERMLINE": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VARLOCIRAPTOR_CALLVARIANTS": {
                     "varlociraptor": "8.9.5"
diff --git a/tests/qc_ngscheckmate.nf.test.snap b/tests/qc_ngscheckmate.nf.test.snap
index 44d732ff4b..e780d6e183 100644
--- a/tests/qc_ngscheckmate.nf.test.snap
+++ b/tests/qc_ngscheckmate.nf.test.snap
@@ -19,12 +19,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -143,12 +143,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/save_mapped.nf.test.snap b/tests/save_mapped.nf.test.snap
index 8467fabb4a..1c750f618f 100644
--- a/tests/save_mapped.nf.test.snap
+++ b/tests/save_mapped.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -58,10 +58,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:34:29.034973351",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T21:27:11.108509"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/save_output_as_bam.nf.test.snap b/tests/save_output_as_bam.nf.test.snap
index 491d13b64b..941145c26d 100644
--- a/tests/save_output_as_bam.nf.test.snap
+++ b/tests/save_output_as_bam.nf.test.snap
@@ -14,22 +14,23 @@
                     "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CREATE_INTERVALS_BED": {
                     "gawk": "5.3.0"
@@ -64,12 +65,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -373,7 +374,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:27:46.994496963",
+        "timestamp": "2026-07-28T14:39:40.512178305",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -403,12 +404,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -435,11 +436,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:39:25.431547577",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-03-13T09:02:14.635985"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --save_output_as_bam --tools sentieon_dedup": {
         "content": [
@@ -455,12 +456,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -490,10 +491,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-28T10:59:38.19303281",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2026-06-09T14:40:13.000000"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/sentieon.nf.test.snap b/tests/sentieon.nf.test.snap
index a14708ec3f..1624728967 100644
--- a/tests/sentieon.nf.test.snap
+++ b/tests/sentieon.nf.test.snap
@@ -39,12 +39,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/sentieon_aligner_bwamem.nf.test.snap b/tests/sentieon_aligner_bwamem.nf.test.snap
index 516178e64a..fe7d2886ca 100644
--- a/tests/sentieon_aligner_bwamem.nf.test.snap
+++ b/tests/sentieon_aligner_bwamem.nf.test.snap
@@ -26,12 +26,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -79,11 +79,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T11:00:26.802531111",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T10:32:56.848655102"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input fastq_umi.csv --aligner sentieon-bwamem --umi_location read2 --umi_length 7 --umi_base_skip 1 --tools sentieon_dedup": {
         "content": [
@@ -112,12 +112,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -156,11 +156,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_umi_cleanname_1.fastq.gz): Cannot extract flowcell ID from @922332"
             ]
         ],
+        "timestamp": "2026-07-28T14:28:02.089129479",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-08-18T06:00:05.261761108"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --aligner sentieon-bwamem --save_reference --build_only_index": {
         "content": [
@@ -176,12 +176,12 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -219,10 +219,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-28T10:59:35.355041067",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-06-21T10:38:04.521781501"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/sentieon_dedup.nf.test.snap b/tests/sentieon_dedup.nf.test.snap
index 4c73072939..8a51af98de 100644
--- a/tests/sentieon_dedup.nf.test.snap
+++ b/tests/sentieon_dedup.nf.test.snap
@@ -29,12 +29,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -186,7 +186,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:35:39.37304294",
+        "timestamp": "2026-07-28T10:57:26.744377427",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -222,12 +222,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -379,7 +379,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:37:42.701914376",
+        "timestamp": "2026-07-28T11:00:34.291406145",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -415,12 +415,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -572,7 +572,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:34:59.34095366",
+        "timestamp": "2026-07-28T11:01:09.715185863",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -611,12 +611,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -768,7 +768,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:36:17.840418937",
+        "timestamp": "2026-07-28T10:58:09.138069802",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -807,12 +807,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/spark.nf.test.snap b/tests/spark.nf.test.snap
index 3c1cb4afba..01afd1e775 100644
--- a/tests/spark.nf.test.snap
+++ b/tests/spark.nf.test.snap
@@ -45,12 +45,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -282,12 +282,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -324,10 +324,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:32:00.112214223",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.10.2"
-        },
-        "timestamp": "2025-12-15T21:33:59.148271232"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/start_from_markduplicates.nf.test.snap b/tests/start_from_markduplicates.nf.test.snap
index 6147c20e10..6d87c3fd7a 100644
--- a/tests/start_from_markduplicates.nf.test.snap
+++ b/tests/start_from_markduplicates.nf.test.snap
@@ -30,12 +30,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -218,12 +218,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -369,7 +369,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:40:21.440602423",
+        "timestamp": "2026-07-28T11:25:22.044646175",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -402,12 +402,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -560,12 +560,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/start_from_preparerecalibration.nf.test.snap b/tests/start_from_preparerecalibration.nf.test.snap
index e6c65e20ee..4f3cbd0fd8 100644
--- a/tests/start_from_preparerecalibration.nf.test.snap
+++ b/tests/start_from_preparerecalibration.nf.test.snap
@@ -26,12 +26,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -164,12 +164,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -275,11 +275,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-28T14:40:17.581153664",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:51:32.157567526"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_bam.csv --step prepare_recalibration --skip_tools baserecalibrator --tools strelka": {
         "content": [
@@ -298,12 +298,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -409,11 +409,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-28T11:02:21.993365701",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:50:33.221939876"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step prepare_recalibration --tools null": {
         "content": [
@@ -442,12 +442,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -557,7 +557,7 @@
             "No VCF files",
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:27:50.357982281",
+        "timestamp": "2026-07-28T11:06:00.25230405",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/start_from_recalibration.nf.test.snap b/tests/start_from_recalibration.nf.test.snap
index 3c4d4773b6..5c101af370 100644
--- a/tests/start_from_recalibration.nf.test.snap
+++ b/tests/start_from_recalibration.nf.test.snap
@@ -23,12 +23,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -144,12 +144,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -255,11 +255,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-28T14:35:23.521371406",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:55:41.4762897"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_bam.csv --step recalibrate --skip_tools baserecalibrator --tools strelka": {
         "content": [
@@ -278,12 +278,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -389,11 +389,11 @@
             ],
             "No warnings"
         ],
+        "timestamp": "2026-07-28T14:35:07.543185264",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T21:54:41.033469111"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input tests/csv/3.0/mapped_single_cram.csv --step recalibrate --tools null": {
         "content": [
@@ -419,12 +419,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/tumor-normal-pair.nf.test.snap b/tests/tumor-normal-pair.nf.test.snap
index 6c664c0a80..7d875b89ac 100644
--- a/tests/tumor-normal-pair.nf.test.snap
+++ b/tests/tumor-normal-pair.nf.test.snap
@@ -49,12 +49,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/umi_fastp.nf.test.snap b/tests/umi_fastp.nf.test.snap
index e15a93581a..ce96888e5a 100644
--- a/tests/umi_fastp.nf.test.snap
+++ b/tests/umi_fastp.nf.test.snap
@@ -46,12 +46,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/umi_fgbio.nf.test.snap b/tests/umi_fgbio.nf.test.snap
index 62a63cbb95..ee15bad18b 100644
--- a/tests/umi_fgbio.nf.test.snap
+++ b/tests/umi_fgbio.nf.test.snap
@@ -97,12 +97,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/umi_in_read_names.nf.test.snap b/tests/umi_in_read_names.nf.test.snap
index 3b94ff9558..8db79c2974 100644
--- a/tests/umi_in_read_names.nf.test.snap
+++ b/tests/umi_in_read_names.nf.test.snap
@@ -39,12 +39,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -342,12 +342,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -635,7 +635,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:42:25.348630085",
+        "timestamp": "2026-07-28T11:27:38.221141795",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_all.nf.test.snap b/tests/variant_calling_all.nf.test.snap
index 6402ccd10b..e1f3c758c8 100644
--- a/tests/variant_calling_all.nf.test.snap
+++ b/tests/variant_calling_all.nf.test.snap
@@ -20,22 +20,23 @@
                     "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -92,22 +93,24 @@
                     "svdb": "2.8.4"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
@@ -590,7 +593,7 @@
                 "test.targetcoverage.cnn:md5,0e0416a5dc409a9c311232092a058aec",
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.target.bed:md5,d7fcdfe7dc055924fe7cc3fb990f5633",
-                "reference.cnn:md5,6542568cd3859ba3789c543c27d64b20",
+                "reference.cnn:md5,2a397362f7f209c4bae39bb18b74e57f",
                 "test.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
                 "test.targetcoverage.cnn:md5,bc82e785b5930b589c216516d1543985",
                 "test2.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
@@ -641,7 +644,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:30:48.429722692",
+        "timestamp": "2026-07-28T14:46:50.973045461",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -668,22 +671,23 @@
                     "samtools": "1.22.1"
                 },
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -727,22 +731,24 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
@@ -1142,7 +1148,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:32:11.483735223",
+        "timestamp": "2026-07-28T11:10:08.379344895",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -1172,13 +1178,14 @@
                     "gatk4": "4.6.2.0"
                 },
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -1228,22 +1235,24 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
@@ -1607,7 +1616,7 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
-        "timestamp": "2026-07-27T10:27:19.8576204",
+        "timestamp": "2026-07-28T11:06:54.492117039",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_ascat.nf.test.snap b/tests/variant_calling_ascat.nf.test.snap
index ac5d21fc53..74c6dfac4c 100644
--- a/tests/variant_calling_ascat.nf.test.snap
+++ b/tests/variant_calling_ascat.nf.test.snap
@@ -14,8 +14,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -109,8 +109,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -205,8 +205,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -357,8 +357,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
diff --git a/tests/variant_calling_cnvkit.nf.test.snap b/tests/variant_calling_cnvkit.nf.test.snap
index c52d3d2793..6655c5a6bd 100644
--- a/tests/variant_calling_cnvkit.nf.test.snap
+++ b/tests/variant_calling_cnvkit.nf.test.snap
@@ -4,22 +4,23 @@
             20,
             {
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -35,12 +36,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -179,7 +180,7 @@
                 "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,4153756b30abc934f10717c023bd262f",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f9e727147a7cbca46c2b9b2de3ecae91",
-                "reference.cnn:md5,e0aefc1c8a40e6919dc9cfb686e51f7c",
+                "reference.cnn:md5,dfc49a037a468bef43a33b5dd4a2bc05",
                 "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
                 "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
                 "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
@@ -199,7 +200,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:30:55.546225902",
+        "timestamp": "2026-07-28T14:42:57.173889218",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -210,22 +211,23 @@
             30,
             {
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -241,12 +243,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -435,7 +437,7 @@
                 "test2.paired_end.recalibrated.sorted.tumor_only.call.cns:md5,70a308c6db7acf1a5fd623936cac6412",
                 "multi_intervals.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "multi_intervals.target.bed:md5,f9e727147a7cbca46c2b9b2de3ecae91",
-                "reference.cnn:md5,e0aefc1c8a40e6919dc9cfb686e51f7c",
+                "reference.cnn:md5,dfc49a037a468bef43a33b5dd4a2bc05",
                 "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
                 "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,826087b08d380135a271e2774c94084c",
                 "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
@@ -456,7 +458,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:28:58.93483756",
+        "timestamp": "2026-07-28T14:41:59.048184914",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -467,22 +469,23 @@
             13,
             {
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -498,12 +501,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -629,22 +632,23 @@
             13,
             {
                 "CNVKIT_ANTITARGET": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_REFERENCE": {
-                    "cnvkit": "0.9.11"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -660,12 +664,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -799,8 +803,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -899,16 +903,17 @@
             16,
             {
                 "CNVKIT_BATCH": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12",
+                    "samtools": "1.21"
                 },
                 "CNVKIT_CALL": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_EXPORT": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CNVKIT_GENEMETRICS": {
-                    "cnvkit": "0.9.10"
+                    "cnvkit": "0.9.12"
                 },
                 "CRAM_TO_BAM": {
                     "samtools": "1.24"
@@ -921,8 +926,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -1068,7 +1073,7 @@
                 "genome.antitarget.bed:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.bed:md5,9c6cc178da8c2c27364be9f25c9df96d",
                 "genome.target.bed:md5,ee8081becc36524d35889e3b5f70961b",
-                "reference.cnn:md5,8c53491ff76a2a06b7a977714db862bb",
+                "reference.cnn:md5,b43c5b91bf2da9efe4dc9631cbb46c44",
                 "test.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
                 "test.paired_end.recalibrated.sorted.targetcoverage.cnn:md5,0067cc3a0e479b23ab3bf056cead31b4",
                 "test2.paired_end.recalibrated.sorted.antitargetcoverage.cnn:md5,203caf8cef6935bb50b4138097955cb8",
@@ -1088,7 +1093,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:27:01.74112415",
+        "timestamp": "2026-07-28T14:38:02.484867231",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_controlfreec.nf.test.snap b/tests/variant_calling_controlfreec.nf.test.snap
index e765355a00..a719e89661 100644
--- a/tests/variant_calling_controlfreec.nf.test.snap
+++ b/tests/variant_calling_controlfreec.nf.test.snap
@@ -29,8 +29,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_CHR_DIR": {
                     "untar": "1.34"
@@ -183,12 +183,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_CHR_DIR": {
                     "untar": "1.34"
@@ -359,8 +359,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_CHR_DIR": {
                     "untar": "1.34"
@@ -474,12 +474,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_CHR_DIR": {
                     "untar": "1.34"
diff --git a/tests/variant_calling_deepvariant.nf.test.snap b/tests/variant_calling_deepvariant.nf.test.snap
index c04371b448..a1855d1a72 100644
--- a/tests/variant_calling_deepvariant.nf.test.snap
+++ b/tests/variant_calling_deepvariant.nf.test.snap
@@ -23,12 +23,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -185,7 +185,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:44:10.97242938",
+        "timestamp": "2026-07-28T11:29:42.272387988",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -214,12 +214,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -365,8 +365,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -516,8 +516,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_freebayes.nf.test.snap b/tests/variant_calling_freebayes.nf.test.snap
index 1fdbe9b711..9ef3934a5a 100644
--- a/tests/variant_calling_freebayes.nf.test.snap
+++ b/tests/variant_calling_freebayes.nf.test.snap
@@ -29,15 +29,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -261,14 +262,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -685,15 +688,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -999,7 +1003,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:29:15.12436469",
+        "timestamp": "2026-07-28T11:08:49.193958384",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -1049,14 +1053,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -1387,14 +1393,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -1627,15 +1635,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_VC_FREEBAYES_FILT": {
-                    "tabix": 1.21
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFLIB_VCFFILTER": {
                     "vcflib": "1.0.14"
@@ -2005,7 +2014,7 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
-        "timestamp": "2026-07-27T10:34:34.523815105",
+        "timestamp": "2026-07-28T11:12:34.424513234",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_haplotypecaller.nf.test.snap b/tests/variant_calling_haplotypecaller.nf.test.snap
index 55281a9cb3..2632ec645a 100644
--- a/tests/variant_calling_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_haplotypecaller.nf.test.snap
@@ -17,8 +17,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -207,12 +207,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -404,8 +404,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -603,12 +603,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_lofreq.nf.test.snap b/tests/variant_calling_lofreq.nf.test.snap
index 74a9f15e41..a2287d7623 100644
--- a/tests/variant_calling_lofreq.nf.test.snap
+++ b/tests/variant_calling_lofreq.nf.test.snap
@@ -23,12 +23,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -212,8 +212,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_manta.nf.test.snap b/tests/variant_calling_manta.nf.test.snap
index c9a50c3d9a..924dbbdc20 100644
--- a/tests/variant_calling_manta.nf.test.snap
+++ b/tests/variant_calling_manta.nf.test.snap
@@ -20,12 +20,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -153,7 +153,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:45:08.642654705",
+        "timestamp": "2026-07-28T11:30:49.321843773",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -177,8 +177,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -336,8 +336,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -531,12 +531,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -664,7 +664,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:35:30.836798406",
+        "timestamp": "2026-07-28T11:13:30.894533486",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -697,12 +697,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -935,12 +935,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -1104,7 +1104,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:30:18.506893012",
+        "timestamp": "2026-07-28T11:09:53.563178736",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -1128,8 +1128,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_mpileup.nf.test.snap b/tests/variant_calling_mpileup.nf.test.snap
index cd66e0b5f8..eb9e8f22e5 100644
--- a/tests/variant_calling_mpileup.nf.test.snap
+++ b/tests/variant_calling_mpileup.nf.test.snap
@@ -20,12 +20,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -207,8 +207,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -393,8 +393,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -582,12 +582,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_msisensor2.nf.test.snap b/tests/variant_calling_msisensor2.nf.test.snap
index 570a51c0f9..4ef1079ca2 100644
--- a/tests/variant_calling_msisensor2.nf.test.snap
+++ b/tests/variant_calling_msisensor2.nf.test.snap
@@ -7,8 +7,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
                     "untar": "1.34"
@@ -61,7 +61,7 @@
             [
                 "multiqc_citations.txt:md5,4c806e63a283ec1b7e78cdae3a923d4f",
                 "genome.dict:md5,de4ff13287d28c1679a11ae989a3a980",
-                "no_intervals.bed.gz:md5,f3dac01ea66b95fe477446fde2d31489",
+                "no_intervals.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "016a16e12aca2bdba3713a3be76f72cd:md5,26ddcb7b64f43835f254a13b29adf3fc",
                 "02d42c2bda19aac304d6e86390c7f328:md5,15ab43fe84b8b7169979d62e267e2b5f",
                 "1030c0aa35ca5c263daeae866ad18632:md5,41e6de8e7e35f66b691072d9839e128a",
@@ -86,11 +86,11 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-28T14:39:49.554362689",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.6"
-        },
-        "timestamp": "2025-10-13T12:34:18.918526"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --tools msisensor2 --build_only_index --input false -stub": {
         "content": [
@@ -100,8 +100,8 @@
                     "gatk4": "4.6.2.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
                     "untar": "1.34"
@@ -177,12 +177,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "UNTAR_MSISENSOR2_MODELS": {
                     "untar": "1.34"
diff --git a/tests/variant_calling_msisensorpro.nf.test.snap b/tests/variant_calling_msisensorpro.nf.test.snap
index e5d36cb0d3..633a663f80 100644
--- a/tests/variant_calling_msisensorpro.nf.test.snap
+++ b/tests/variant_calling_msisensorpro.nf.test.snap
@@ -10,8 +10,8 @@
                     "msisensor-pro": "1.3.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -69,12 +69,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -206,8 +206,8 @@
                     "msisensor-pro": "1.3.0"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 }
             },
             [
@@ -239,7 +239,7 @@
             [
                 "multiqc_citations.txt:md5,4c806e63a283ec1b7e78cdae3a923d4f",
                 "genome.dict:md5,de4ff13287d28c1679a11ae989a3a980",
-                "no_intervals.bed.gz:md5,f3dac01ea66b95fe477446fde2d31489",
+                "no_intervals.bed.gz:md5,d41d8cd98f00b204e9800998ecf8427e",
                 "genome.msisensor_scan.list:md5,614754c7f1f44d5988dd80f8f21f69d1"
             ],
             "No BAM files",
@@ -247,10 +247,10 @@
             "No VCF files",
             "No warnings"
         ],
+        "timestamp": "2026-07-28T14:41:52.061061413",
         "meta": {
-            "nf-test": "0.9.3",
-            "nextflow": "25.04.7"
-        },
-        "timestamp": "2025-09-30T23:19:39.916442214"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_muse.nf.test.snap b/tests/variant_calling_muse.nf.test.snap
index 68982cfc30..b03adbde8a 100644
--- a/tests/variant_calling_muse.nf.test.snap
+++ b/tests/variant_calling_muse.nf.test.snap
@@ -29,12 +29,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -210,12 +210,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_mutect2.nf.test.snap b/tests/variant_calling_mutect2.nf.test.snap
index 0a33bc5519..ae1086a913 100644
--- a/tests/variant_calling_mutect2.nf.test.snap
+++ b/tests/variant_calling_mutect2.nf.test.snap
@@ -23,8 +23,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -220,12 +220,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -408,7 +408,7 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
-        "timestamp": "2026-07-27T10:49:05.16697888",
+        "timestamp": "2026-07-28T11:35:37.049228309",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -438,8 +438,8 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -649,12 +649,12 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -820,7 +820,7 @@
                 "WARN: No Panel-of-normal was specified for Mutect2."
             ]
         ],
-        "timestamp": "2026-07-27T10:33:25.493676415",
+        "timestamp": "2026-07-28T11:12:58.603126898",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_sentieon_dnascope.nf.test.snap b/tests/variant_calling_sentieon_dnascope.nf.test.snap
index 6aae5c5a95..0157cc0068 100644
--- a/tests/variant_calling_sentieon_dnascope.nf.test.snap
+++ b/tests/variant_calling_sentieon_dnascope.nf.test.snap
@@ -23,12 +23,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -186,7 +186,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:38:52.499416464",
+        "timestamp": "2026-07-28T11:00:49.396195515",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -219,12 +219,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -385,7 +385,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:34:52.637438538",
+        "timestamp": "2026-07-28T11:01:09.004361032",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -424,12 +424,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -593,7 +593,7 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
-        "timestamp": "2026-07-27T11:36:02.196958675",
+        "timestamp": "2026-07-28T11:01:41.929110424",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
index f861d9b5b2..016bbdb2bf 100644
--- a/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
+++ b/tests/variant_calling_sentieon_haplotypecaller.nf.test.snap
@@ -44,12 +44,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -216,7 +216,7 @@
                 "WARN: If GATK's Haplotypecaller, Sentieon's Dnascope and/or Sentieon's Haplotyper is specified, but without `--dbsnp`, `--known_snps`, `--known_indels` or the associated resource labels (ie `known_snps_vqsr`), no variant recalibration will be done. For recalibration you must provide all of these resources."
             ]
         ],
-        "timestamp": "2026-07-27T11:36:08.094597613",
+        "timestamp": "2026-07-28T11:02:16.293619207",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -246,8 +246,8 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -441,12 +441,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -604,7 +604,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:36:30.915587402",
+        "timestamp": "2026-07-28T10:58:16.960328143",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -640,12 +640,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -806,7 +806,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T11:36:24.959312656",
+        "timestamp": "2026-07-28T11:01:47.702910053",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
diff --git a/tests/variant_calling_sentieon_tnscope.nf.test.snap b/tests/variant_calling_sentieon_tnscope.nf.test.snap
index 924500df0d..e8dfa5b094 100644
--- a/tests/variant_calling_sentieon_tnscope.nf.test.snap
+++ b/tests/variant_calling_sentieon_tnscope.nf.test.snap
@@ -23,12 +23,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -86,11 +86,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998513#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T10:59:00.525116855",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-07-03T13:19:32.295689002"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input fastq_triple_two_tumor.csv --tools sentieon_tnscope": {
         "content": [
@@ -116,12 +116,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -205,11 +205,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T11:02:18.439914438",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-07-03T19:24:18.564064846"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input fastq_pair.csv --tools sentieon_tnscope --no_intervals": {
         "content": [
@@ -229,8 +229,8 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -299,11 +299,11 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T14:29:54.161693908",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-07-03T13:12:22.769869621"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     },
     "-profile test --input fastq_pair.csv --tools sentieon_tnscope": {
         "content": [
@@ -329,12 +329,12 @@
                     "sentieon": "202503.02"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -400,10 +400,10 @@
                 "WARN: FASTQ file(/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz): Cannot extract flowcell ID from @normal#21#998579#1/1"
             ]
         ],
+        "timestamp": "2026-07-28T11:01:53.081165903",
         "meta": {
-            "nf-test": "0.9.2",
-            "nextflow": "25.04.2"
-        },
-        "timestamp": "2025-07-03T13:39:14.931074918"
+            "nf-test": "0.9.5",
+            "nextflow": "25.10.4"
+        }
     }
 }
diff --git a/tests/variant_calling_strelka.nf.test.snap b/tests/variant_calling_strelka.nf.test.snap
index 5a8093d26e..bf17d619f2 100644
--- a/tests/variant_calling_strelka.nf.test.snap
+++ b/tests/variant_calling_strelka.nf.test.snap
@@ -23,12 +23,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -252,12 +252,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -444,12 +444,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -672,7 +672,7 @@
             ],
             "No warnings"
         ],
-        "timestamp": "2026-07-27T10:37:04.502091857",
+        "timestamp": "2026-07-28T11:15:18.903995357",
         "meta": {
             "nf-test": "0.9.5",
             "nextflow": "25.10.4"
@@ -696,8 +696,8 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -884,8 +884,8 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_strelka_bp.nf.test.snap b/tests/variant_calling_strelka_bp.nf.test.snap
index 9ffbc319f1..67db423b2b 100644
--- a/tests/variant_calling_strelka_bp.nf.test.snap
+++ b/tests/variant_calling_strelka_bp.nf.test.snap
@@ -26,8 +26,8 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
@@ -302,12 +302,12 @@
                     "strelka": "2.9.10"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "VCFTOOLS_SUMMARY": {
                     "vcftools": "0.1.17"
diff --git a/tests/variant_calling_tiddit.nf.test.snap b/tests/variant_calling_tiddit.nf.test.snap
index 09bb83af3f..96d018d114 100644
--- a/tests/variant_calling_tiddit.nf.test.snap
+++ b/tests/variant_calling_tiddit.nf.test.snap
@@ -17,16 +17,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
@@ -182,16 +182,16 @@
                     "samtools": "1.24"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
@@ -351,16 +351,16 @@
                     "svdb": "2.8.4"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TABIX_BGZIP_TIDDIT_SV": {
-                    "bgzip": "1.21",
-                    "tabix": "1.21"
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
                 },
                 "TIDDIT_SV": {
                     "tiddit": "3.9.5"
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 5df7d648d1..18611e17ef 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -542,7 +542,6 @@ workflow SAREK {
         // Gather used variant calling softwares versions
         versions = versions.mix(BAM_VARIANT_CALLING_GERMLINE_ALL.out.versions)
         versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_ALL.out.versions)
-        versions = versions.mix(BAM_VARIANT_CALLING_TUMOR_ONLY_ALL.out.versions)
         versions = versions.mix(POST_VARIANTCALLING.out.versions)
 
         // ANNOTATE
@@ -571,9 +570,6 @@ workflow SAREK {
                 bcftools_header_lines,
                 snpsift_db,
             )
-
-            // Gather used softwares versions
-            versions = versions.mix(VCF_ANNOTATE_ALL.out.versions)
         }
     }
 

From 9f7ca0be040533aecc35be506d73b51699b61a9d Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Wed, 29 Jul 2026 12:23:56 +0200
Subject: [PATCH 20/27] chore(modules): migrate local modules to versions topic
 channel (#2244)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Migrates the local modules to the `versions` topic channel. **Stacked on
#2243** (base: `topic/cnvkit`).

### Changes
- Converts `add_info_to_vcf`, `create_intervals_bed` and
`samtools/reindex_bam` from `versions.yml` to the versions topic
channel, matching the `consensus_from_sites` pattern.
- Removes the corresponding `.out.versions` wiring.

No tool version changes.

🤖 Generated with [Claude Code](https://claude.com/claude-code)

---------

Co-authored-by: Claude Opus 4.8 (1M context) <noreply@anthropic.com>
---
 CHANGELOG.md                                  |   1 +
 main.nf                                       |   5 -
 modules/local/add_info_to_vcf/main.nf         |   7 +-
 modules/local/add_info_to_vcf/meta.yml        |  59 +++++++
 modules/local/create_intervals_bed/main.nf    |  22 +--
 modules/local/create_intervals_bed/meta.yml   |  57 +++++++
 modules/local/samtools/reindex_bam/main.nf    |   7 +-
 modules/local/samtools/reindex_bam/meta.yml   |  89 +++++++++++
 .../bam_variant_calling_germline_all/main.nf  |   5 -
 .../bam_variant_calling_indexcov/main.nf      |   5 -
 .../bam_variant_calling_somatic_all/main.nf   |   5 -
 .../local/post_variantcalling/main.nf         |   5 -
 .../post_variantcalling/tests/main.nf.test    |  42 ++---
 .../tests/main.nf.test.snap                   | 144 +-----------------
 subworkflows/local/prepare_intervals/main.nf  |   7 -
 .../local/utils_nfcore_sarek_pipeline/main.nf |   3 -
 .../local/vcf_concatenate_germline/main.nf    |   6 -
 subworkflows/local/vcf_normalization/main.nf  |   6 -
 workflows/sarek.nf                            |  11 +-
 19 files changed, 232 insertions(+), 254 deletions(-)
 create mode 100644 modules/local/add_info_to_vcf/meta.yml
 create mode 100644 modules/local/create_intervals_bed/meta.yml
 create mode 100644 modules/local/samtools/reindex_bam/meta.yml

diff --git a/CHANGELOG.md b/CHANGELOG.md
index b815a4a08f..5e3f69d1b1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -24,6 +24,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2241](https://github.com/nf-core/sarek/pull/2241) - Migrate `samtools/*` and `mosdepth` modules to the versions topic channel
 - [#2242](https://github.com/nf-core/sarek/pull/2242) - Migrate QC/coverage modules (`ascat`, `goleft`, `msisensor2`, `msisensorpro`) to the versions topic channel
 - [#2243](https://github.com/nf-core/sarek/pull/2243) - Migrate `cnvkit/*` modules to the versions topic channel, and replace the deprecated `tabix/tabix`/`tabix/bgziptabix` modules with `htslib/bgziptabix`
+- [#2244](https://github.com/nf-core/sarek/pull/2244) - Migrate local modules (`add_info_to_vcf`, `create_intervals_bed`, `samtools/reindex_bam`) to the versions topic channel
 
 ### Fixed
 
diff --git a/main.nf b/main.nf
index dadcb67358..1ea13f281a 100755
--- a/main.nf
+++ b/main.nf
@@ -90,8 +90,6 @@ workflow NFCORE_SAREK {
     samplesheet
 
     main:
-    versions = channel.empty()
-
     // build indexes if needed
     PREPARE_GENOME(
         params.ascat_alleles,
@@ -165,8 +163,6 @@ workflow NFCORE_SAREK {
     else {
         cnvkit_reference = channel.value([])
     }
-    // Gather used softwares versions
-    versions = versions.mix(PREPARE_INTERVALS.out.versions)
 
     // Fails when consensus calling is specified without normalization
     if (params.snv_consensus_calling && !params.normalize_vcfs) {
@@ -337,7 +333,6 @@ workflow NFCORE_SAREK {
         params.vep_genome,
         params.vep_species,
         ch_snpsift_db,
-        versions,
     )
 
     emit:
diff --git a/modules/local/add_info_to_vcf/main.nf b/modules/local/add_info_to_vcf/main.nf
index 466c33f15b..252dfb49ff 100644
--- a/modules/local/add_info_to_vcf/main.nf
+++ b/modules/local/add_info_to_vcf/main.nf
@@ -12,7 +12,7 @@ process ADD_INFO_TO_VCF {
 
     output:
     tuple val(meta), path("*.added_info.vcf"), emit: vcf
-    path "versions.yml",                       emit: versions
+    tuple val("${task.process}"), val('gawk'), eval("awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'"), emit: versions_gawk, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -32,10 +32,5 @@ process ADD_INFO_TO_VCF {
     if grep -Ev "^#" \$input; then
         grep -Ev "^#" \$input | awk 'BEGIN{FS=OFS="\t"} { \$8=="." ? \$8="SOURCE=${vcf_gz}" : \$8=\$8";SOURCE=${vcf_gz}"; print }' >> \$output
     fi
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-    END_VERSIONS
     """
 }
diff --git a/modules/local/add_info_to_vcf/meta.yml b/modules/local/add_info_to_vcf/meta.yml
new file mode 100644
index 0000000000..69e66eeb8a
--- /dev/null
+++ b/modules/local/add_info_to_vcf/meta.yml
@@ -0,0 +1,59 @@
+name: "add_info_to_vcf"
+description: Add a SOURCE INFO tag to every variant record in a VCF, naming the file it came from
+keywords:
+  - vcf
+  - annotation
+  - info
+tools:
+  - "gawk":
+      description: "GNU awk, a pattern scanning and processing language"
+      homepage: "https://www.gnu.org/software/gawk/"
+      documentation: "https://www.gnu.org/software/gawk/manual/gawk.html"
+      licence: ["GPL-3.0-or-later"]
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'sample1' ]
+    - vcf_gz:
+        type: file
+        description: Input VCF file, optionally gzipped
+        pattern: "*.{vcf,vcf.gz}"
+output:
+  vcf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'sample1' ]
+      - "*.added_info.vcf":
+          type: file
+          description: VCF file with the SOURCE INFO tag added to every variant record
+          pattern: "*.added_info.vcf"
+  versions_gawk:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - "awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - "awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'":
+          type: eval
+          description: The expression to obtain the version of the tool
+authors:
+  - "@asp8200"
+maintainers:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
diff --git a/modules/local/create_intervals_bed/main.nf b/modules/local/create_intervals_bed/main.nf
index 3ffe9a7428..f7a70c1e6c 100644
--- a/modules/local/create_intervals_bed/main.nf
+++ b/modules/local/create_intervals_bed/main.nf
@@ -13,7 +13,7 @@ process CREATE_INTERVALS_BED {
 
     output:
     path ("*.bed"),      emit: bed
-    path "versions.yml", emit: versions
+    tuple val("${task.process}"), val('gawk'), eval("awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'"), emit: versions_gawk, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -41,11 +41,6 @@ process CREATE_INTERVALS_BED {
             chunk += t
             print \$0 > name
         }' ${intervals}
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-        END_VERSIONS
         """
     }
     else if (intervals.toString().toLowerCase().endsWith("interval_list")) {
@@ -54,11 +49,6 @@ process CREATE_INTERVALS_BED {
             name = sprintf("%s_%d-%d", \$1, \$2, \$3);
             printf("%s\\t%d\\t%d\\n", \$1, \$2-1, \$3) > name ".bed"
         }'
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-        END_VERSIONS
         """
     }
     else {
@@ -67,11 +57,6 @@ process CREATE_INTERVALS_BED {
             name = sprintf("%s_%d-%d", \$1, \$2, \$3);
             printf("%s\\t%d\\t%d\\n", \$1, \$2-1, \$3) > name ".bed"
         }' ${intervals}
-
-        cat <<-END_VERSIONS > versions.yml
-        "${task.process}":
-            gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-        END_VERSIONS
         """
     }
 
@@ -79,10 +64,5 @@ process CREATE_INTERVALS_BED {
     def prefix = task.ext.prefix ?: "${intervals.baseName}"
     """
     touch ${prefix}.stub.bed
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        gawk: \$(awk -Wversion | sed '1!d; s/.*Awk //; s/,.*//')
-    END_VERSIONS
     """
 }
diff --git a/modules/local/create_intervals_bed/meta.yml b/modules/local/create_intervals_bed/meta.yml
new file mode 100644
index 0000000000..926e3f743b
--- /dev/null
+++ b/modules/local/create_intervals_bed/meta.yml
@@ -0,0 +1,57 @@
+name: "create_intervals_bed"
+description: Split an interval file (BED, GATK interval_list, or picard-style `chr:start-end`
+  list) into runtime-balanced BED chunks for parallelization
+keywords:
+  - intervals
+  - bed
+  - parallelization
+tools:
+  - "gawk":
+      description: "GNU awk, a pattern scanning and processing language"
+      homepage: "https://www.gnu.org/software/gawk/"
+      documentation: "https://www.gnu.org/software/gawk/manual/gawk.html"
+      licence: ["GPL-3.0-or-later"]
+input:
+  - - intervals:
+        type: file
+        description: Interval file, either BED, GATK `.interval_list`, or a picard-style
+          `chr:start-end` list
+        pattern: "*.{bed,interval_list}"
+    - nucleotides_per_second:
+        type: integer
+        description: Estimated nucleotides processed per second, used to balance runtime
+          across chunks when the input has no runtime estimate column
+output:
+  bed:
+    - "*.bed":
+        type: file
+        description: One or more BED files, each a runtime-balanced chunk of the input
+          intervals
+        pattern: "*.bed"
+  versions_gawk:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - "awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - gawk:
+          type: string
+          description: The name of the tool
+      - "awk --version | head -n1 | sed 's/GNU Awk //; s/, .*//'":
+          type: eval
+          description: The expression to obtain the version of the tool
+authors:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
+maintainers:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
diff --git a/modules/local/samtools/reindex_bam/main.nf b/modules/local/samtools/reindex_bam/main.nf
index 153f9093d6..d4503cb1a6 100644
--- a/modules/local/samtools/reindex_bam/main.nf
+++ b/modules/local/samtools/reindex_bam/main.nf
@@ -18,7 +18,7 @@ process SAMTOOLS_REINDEX_BAM {
 
     output:
     tuple val(meta), path("${meta.id}.reindex.bam"), path("${meta.id}.reindex.bam.bai"),emit: output
-    path  "versions.yml"            , emit: versions
+    tuple val("${task.process}"), val('samtools'), eval("samtools --version | head -n1 | sed 's/samtools //'"), emit: versions_samtools, topic: versions
 
     when:
     task.ext.when == null || task.ext.when
@@ -48,10 +48,5 @@ process SAMTOOLS_REINDEX_BAM {
         ${reference} \\
         ${args} \\
         ${input}
-
-    cat <<-END_VERSIONS > versions.yml
-    "${task.process}":
-        samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
-    END_VERSIONS
     """
 }
diff --git a/modules/local/samtools/reindex_bam/meta.yml b/modules/local/samtools/reindex_bam/meta.yml
new file mode 100644
index 0000000000..c8ff7973c0
--- /dev/null
+++ b/modules/local/samtools/reindex_bam/meta.yml
@@ -0,0 +1,89 @@
+name: "samtools_reindex_bam"
+description: Re-index a BAM/CRAM after dropping duplicate, supplementary, and unmapped
+  reads, producing a header-only BAM and a matching index for goleft/indexcov
+keywords:
+  - samtools
+  - bam
+  - index
+  - indexcov
+tools:
+  - "samtools":
+      description: "Tools for dealing with SAM, BAM and CRAM files"
+      homepage: "http://www.htslib.org/"
+      documentation: "http://www.htslib.org/doc/samtools.html"
+      tool_dev_url: "https://github.com/samtools/samtools"
+      doi: "10.1093/bioinformatics/btp352"
+      licence: ["MIT"]
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information
+          e.g. [ id:'sample1' ]
+    - input:
+        type: file
+        description: BAM/CRAM file
+        pattern: "*.{bam,cram}"
+    - input_index:
+        type: file
+        description: BAM/CRAM index
+        pattern: "*.{bai,crai}"
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: Reference genome FASTA file, required when the input is CRAM
+        pattern: "*.{fa,fasta}"
+  - - meta3:
+        type: map
+        description: |
+          Groovy Map containing reference information
+          e.g. [ id:'genome' ]
+    - fai:
+        type: file
+        description: Reference genome FASTA index, required when the input is CRAM
+        pattern: "*.fai"
+output:
+  output:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information
+            e.g. [ id:'sample1' ]
+      - "${meta.id}.reindex.bam":
+          type: file
+          description: Header-only BAM with duplicate/supplementary/unmapped reads removed
+          pattern: "*.reindex.bam"
+      - "${meta.id}.reindex.bam.bai":
+          type: file
+          description: BAM index matching the reindexed BAM
+          pattern: "*.reindex.bam.bai"
+  versions_samtools:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - "samtools --version | head -n1 | sed 's/samtools //'":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - "${task.process}":
+          type: string
+          description: The name of the process
+      - samtools:
+          type: string
+          description: The name of the tool
+      - "samtools --version | head -n1 | sed 's/samtools //'":
+          type: eval
+          description: The expression to obtain the version of the tool
+authors:
+  - "@lindenb"
+maintainers:
+  - "@FriederikeHanssen"
+  - "@maxulysse"
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index abea346137..f8278a506d 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -52,8 +52,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     sentieon_dnascope_model           // channel: [mandatory] value channel with string
 
     main:
-    versions = channel.empty()
-
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
     gvcf_sentieon_dnascope       = channel.empty()
     gvcf_sentieon_haplotyper     = channel.empty()
@@ -211,7 +209,6 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         )
 
         out_indexcov = BAM_VARIANT_CALLING_INDEXCOV.out.out_indexcov
-        versions = versions.mix(BAM_VARIANT_CALLING_INDEXCOV.out.versions)
     }
 
     // SENTIEON DNASCOPE
@@ -407,6 +404,4 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     tbi_sentieon_haplotyper
     tbi_strelka
     tbi_tiddit
-
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_indexcov/main.nf b/subworkflows/local/bam_variant_calling_indexcov/main.nf
index 59c261d10c..3b65b1ec70 100644
--- a/subworkflows/local/bam_variant_calling_indexcov/main.nf
+++ b/subworkflows/local/bam_variant_calling_indexcov/main.nf
@@ -15,8 +15,6 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
     fasta_fai     // channel: [mandatory] [ meta, fasta_fai ]
 
     main:
-    versions = channel.empty()
-
     // generate a cleaner bam index without duplicate, supplementary, etc. (Small workload because the bam itself is not re-generated)
     reindex_ch = SAMTOOLS_REINDEX_BAM(
         cram,
@@ -24,8 +22,6 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
         fasta_fai
         )
 
-    versions = versions.mix(reindex_ch.versions)
-
     // create [ [id:directory], bams, bais ]
     indexcov_input_ch = reindex_ch.output.map{ _meta, bam, bai -> [[id:"indexcov"], bam, bai]}.groupTuple()
 
@@ -37,5 +33,4 @@ workflow BAM_VARIANT_CALLING_INDEXCOV {
     emit:
 
     out_indexcov = goleft_ch.output
-    versions
 }
diff --git a/subworkflows/local/bam_variant_calling_somatic_all/main.nf b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
index 5d9a0210f7..4eec1ef6dd 100644
--- a/subworkflows/local/bam_variant_calling_somatic_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_somatic_all/main.nf
@@ -51,9 +51,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
     main:
     // channels are often remapped to match module/subworkflow
 
-    // Gather all versions
-    versions = channel.empty()
-
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
     out_indexcov     = channel.empty()
     out_msisensorpro = channel.empty()
@@ -171,7 +168,6 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
         )
 
         out_indexcov = BAM_VARIANT_CALLING_INDEXCOV.out.out_indexcov
-        versions = versions.mix(BAM_VARIANT_CALLING_INDEXCOV.out.versions)
     }
 
     // STRELKA
@@ -316,5 +312,4 @@ workflow BAM_VARIANT_CALLING_SOMATIC_ALL {
     tbi_strelka
     tbi_tiddit
     tbi_tnscope
-    versions
 }
diff --git a/subworkflows/local/post_variantcalling/main.nf b/subworkflows/local/post_variantcalling/main.nf
index 1dbfd3059c..3dc33dde0c 100644
--- a/subworkflows/local/post_variantcalling/main.nf
+++ b/subworkflows/local/post_variantcalling/main.nf
@@ -37,7 +37,6 @@ workflow POST_VARIANTCALLING {
     varlociraptor_scenario_tumor_only
 
     main:
-    versions = channel.empty()
     vcfs = channel.empty()
     tbis = channel.empty()
 
@@ -124,7 +123,6 @@ workflow POST_VARIANTCALLING {
 
             small_variant_vcfs = NORMALIZE_VCFS.out.vcfs // [meta, vcf]
             small_variant_tbis = NORMALIZE_VCFS.out.tbis // [meta, tbi]
-            versions = versions.mix(NORMALIZE_VCFS.out.versions)
         }
 
         if (normalize_vcfs && snv_consensus_calling){
@@ -156,8 +154,6 @@ workflow POST_VARIANTCALLING {
 
             vcfs = vcfs.mix(CONCATENATE_GERMLINE_VCFS.out.vcfs)
             tbis = tbis.mix(CONCATENATE_GERMLINE_VCFS.out.tbis)
-
-            versions = versions.mix(CONCATENATE_GERMLINE_VCFS.out.versions)
         }
 
 
@@ -169,5 +165,4 @@ workflow POST_VARIANTCALLING {
     emit:
     vcfs     // post processed vcfs [meta, vcf]
     tbis     // post processed tbis [meta, tbi]
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/post_variantcalling/tests/main.nf.test b/subworkflows/local/post_variantcalling/tests/main.nf.test
index 934420065d..67136e3530 100644
--- a/subworkflows/local/post_variantcalling/tests/main.nf.test
+++ b/subworkflows/local/post_variantcalling/tests/main.nf.test
@@ -69,8 +69,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -136,8 +135,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -203,8 +201,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -334,8 +331,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -402,8 +398,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -474,8 +469,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -545,8 +539,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -617,8 +610,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -687,8 +679,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -767,8 +758,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -845,8 +835,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -918,8 +907,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -991,8 +979,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
@@ -1066,8 +1053,7 @@ nextflow_workflow {
                 { assert workflow.success },
                 { assert snapshot(
                     workflow.out.vcfs,
-                    workflow.out.tbis,
-                    workflow.out.versions
+                    workflow.out.tbis
                 ).match() }
             )
         }
diff --git a/subworkflows/local/post_variantcalling/tests/main.nf.test.snap b/subworkflows/local/post_variantcalling/tests/main.nf.test.snap
index fefd5b66a9..17cbb5c205 100644
--- a/subworkflows/local/post_variantcalling/tests/main.nf.test.snap
+++ b/subworkflows/local/post_variantcalling/tests/main.nf.test.snap
@@ -32,18 +32,6 @@
                     },
                     "test_sample.germline.vcf.gz.tbi:md5,eda316880fe501671a66e26aefa7abf6"
                 ]
-            ],
-            [
-                "versions.yml:md5,047305afd4d6a35ccc6c8cae2d0de0cb",
-                "versions.yml:md5,047305afd4d6a35ccc6c8cae2d0de0cb",
-                "versions.yml:md5,594bb4ec971ba178d11d6f4c32fa5398",
-                "versions.yml:md5,594bb4ec971ba178d11d6f4c32fa5398",
-                "versions.yml:md5,b5b952ec9c95cc575f4305901a4af77c",
-                "versions.yml:md5,b5b952ec9c95cc575f4305901a4af77c",
-                "versions.yml:md5,eeffdab3a5d087ebcc8fe19bd27c900f",
-                "versions.yml:md5,eeffdab3a5d087ebcc8fe19bd27c900f",
-                "versions.yml:md5,f9826bc6c76002549649f51bda91a223",
-                "versions.yml:md5,f9826bc6c76002549649f51bda91a223"
             ]
         ],
         "meta": {
@@ -99,25 +87,6 @@
                     },
                     "test_sample.germline.vcf.gz.tbi:md5,eda316880fe501671a66e26aefa7abf6"
                 ]
-            ],
-            [
-                "versions.yml:md5,047305afd4d6a35ccc6c8cae2d0de0cb",
-                "versions.yml:md5,047305afd4d6a35ccc6c8cae2d0de0cb",
-                "versions.yml:md5,594bb4ec971ba178d11d6f4c32fa5398",
-                "versions.yml:md5,594bb4ec971ba178d11d6f4c32fa5398",
-                "versions.yml:md5,6ea9087108ce40c482f810d350240e2e",
-                "versions.yml:md5,8e5af507f2006539314960cc13610d2c",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,b5b952ec9c95cc575f4305901a4af77c",
-                "versions.yml:md5,b5b952ec9c95cc575f4305901a4af77c",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,e79c4d48ba8ecd05bbd54969fa63fd0a",
-                "versions.yml:md5,eeffdab3a5d087ebcc8fe19bd27c900f",
-                "versions.yml:md5,eeffdab3a5d087ebcc8fe19bd27c900f",
-                "versions.yml:md5,f9826bc6c76002549649f51bda91a223",
-                "versions.yml:md5,f9826bc6c76002549649f51bda91a223"
             ]
         ],
         "meta": {
@@ -143,13 +112,6 @@
                     },
                     "test_sample.germline.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
                 ]
-            ],
-            [
-                "versions.yml:md5,047305afd4d6a35ccc6c8cae2d0de0cb",
-                "versions.yml:md5,594bb4ec971ba178d11d6f4c32fa5398",
-                "versions.yml:md5,b5b952ec9c95cc575f4305901a4af77c",
-                "versions.yml:md5,eeffdab3a5d087ebcc8fe19bd27c900f",
-                "versions.yml:md5,f9826bc6c76002549649f51bda91a223"
             ]
         ],
         "meta": {
@@ -191,14 +153,6 @@
                     },
                     "tumor_sample.strelka.tumor_only.varlociraptor.vcf.gz.tbi:md5,6f17310cd152968349269107e53371c2"
                 ]
-            ],
-            [
-                "versions.yml:md5,2944a0f9af0e3918bb9833c87df1ebb6",
-                "versions.yml:md5,29f73f6b28ae158785ebea38843af786",
-                "versions.yml:md5,5d2a1f6080254676bff1ef3b27ab641f",
-                "versions.yml:md5,c35cd6c1892a24d29169bc79e7623bd9",
-                "versions.yml:md5,dc55a53ff7729dc648ac35db31ec5656",
-                "versions.yml:md5,faeabb0df6266c84e0fcd7f39d60b759"
             ]
         ],
         "meta": {
@@ -242,20 +196,6 @@
                     },
                     "tumor_sample.strelka.somatic.varlociraptor.vcf.gz.tbi:md5,25d0445f304a4e9db421b880f866cc2c"
                 ]
-            ],
-            [
-                "versions.yml:md5,106dcb110a6d3d094298500f4030ef8f",
-                "versions.yml:md5,1b666f0fbac8556cef352a3a4fa60dbe",
-                "versions.yml:md5,1bca8763ec1f55aa5987e4f4301ed8cf",
-                "versions.yml:md5,26d247da3c326a0b09dff1bea9c55c52",
-                "versions.yml:md5,31e8ad0d1040730aceafc75e5c9fedac",
-                "versions.yml:md5,3aa2ccd7a5a2d5ecf2afff04a39e0f55",
-                "versions.yml:md5,641cf79d74888d2838e17449cfb5a98c",
-                "versions.yml:md5,65c36101acc269f90fcc762f4c515073",
-                "versions.yml:md5,6b1c3a74fcd23a4a7f78ddf4ef6c6f99",
-                "versions.yml:md5,ae66ca7ac06bbe30c3534b20ade99b48",
-                "versions.yml:md5,d28b191a8b7e7f1db9f269fc372d950e",
-                "versions.yml:md5,e75820d6c821e7da2cd08f5e42c9139e"
             ]
         ],
         "meta": {
@@ -281,17 +221,6 @@
                     },
                     "test_tumor.null.norm.vcf.gz.tbi:md5,4331f1e4e2ba3cd35241c62e6a3e3a69"
                 ]
-            ],
-            [
-                "versions.yml:md5,6ea9087108ce40c482f810d350240e2e",
-                "versions.yml:md5,8e5af507f2006539314960cc13610d2c",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,e79c4d48ba8ecd05bbd54969fa63fd0a"
             ]
         ],
         "meta": {
@@ -302,21 +231,8 @@
     },
     "POST_VARIANTCALLING - varlociraptor germline - stub": {
         "content": [
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,1bca8763ec1f55aa5987e4f4301ed8cf",
-                "versions.yml:md5,25260e5ecf0e3f964c7e68c7eaa01d52",
-                "versions.yml:md5,45997f1ffe1a1c5fceda0f00b670faa8",
-                "versions.yml:md5,5a1892540eb326a685a22f9bc6f6a6b6",
-                "versions.yml:md5,9185ef5a5fa1849e93823a668c488c5c",
-                "versions.yml:md5,97327c4f942c0205e15b8cb4e363825b",
-                "versions.yml:md5,d28b191a8b7e7f1db9f269fc372d950e"
-            ]
+            [],
+            []
         ],
         "meta": {
             "nf-test": "0.9.2",
@@ -326,26 +242,8 @@
     },
     "POST_VARIANTCALLING - varlociraptor somatic - stub": {
         "content": [
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,106dcb110a6d3d094298500f4030ef8f",
-                "versions.yml:md5,1b666f0fbac8556cef352a3a4fa60dbe",
-                "versions.yml:md5,1bca8763ec1f55aa5987e4f4301ed8cf",
-                "versions.yml:md5,26d247da3c326a0b09dff1bea9c55c52",
-                "versions.yml:md5,31e8ad0d1040730aceafc75e5c9fedac",
-                "versions.yml:md5,3aa2ccd7a5a2d5ecf2afff04a39e0f55",
-                "versions.yml:md5,641cf79d74888d2838e17449cfb5a98c",
-                "versions.yml:md5,65c36101acc269f90fcc762f4c515073",
-                "versions.yml:md5,6b1c3a74fcd23a4a7f78ddf4ef6c6f99",
-                "versions.yml:md5,ae66ca7ac06bbe30c3534b20ade99b48",
-                "versions.yml:md5,d28b191a8b7e7f1db9f269fc372d950e",
-                "versions.yml:md5,e75820d6c821e7da2cd08f5e42c9139e"
-            ]
+            [],
+            []
         ],
         "meta": {
             "nf-test": "0.9.2",
@@ -384,15 +282,6 @@
                     },
                     "test_sample.strelka.germline.varlociraptor.vcf.gz.tbi:md5,9e063f81e588cb3f7ad883463c230153"
                 ]
-            ],
-            [
-                "versions.yml:md5,1bca8763ec1f55aa5987e4f4301ed8cf",
-                "versions.yml:md5,25260e5ecf0e3f964c7e68c7eaa01d52",
-                "versions.yml:md5,45997f1ffe1a1c5fceda0f00b670faa8",
-                "versions.yml:md5,5a1892540eb326a685a22f9bc6f6a6b6",
-                "versions.yml:md5,9185ef5a5fa1849e93823a668c488c5c",
-                "versions.yml:md5,97327c4f942c0205e15b8cb4e363825b",
-                "versions.yml:md5,d28b191a8b7e7f1db9f269fc372d950e"
             ]
         ],
         "meta": {
@@ -418,13 +307,6 @@
                     },
                     "test_sample.null.norm.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e"
                 ]
-            ],
-            [
-                "versions.yml:md5,6ea9087108ce40c482f810d350240e2e",
-                "versions.yml:md5,8e5af507f2006539314960cc13610d2c",
-                "versions.yml:md5,af9213a1434d504b9b19d0236f508046",
-                "versions.yml:md5,dee58c88dd03c46f90d7b83578b4ae82",
-                "versions.yml:md5,e79c4d48ba8ecd05bbd54969fa63fd0a"
             ]
         ],
         "meta": {
@@ -435,20 +317,8 @@
     },
     "POST_VARIANTCALLING - varlociraptor tumor-only - stub": {
         "content": [
-            [
-                
-            ],
-            [
-                
-            ],
-            [
-                "versions.yml:md5,2944a0f9af0e3918bb9833c87df1ebb6",
-                "versions.yml:md5,29f73f6b28ae158785ebea38843af786",
-                "versions.yml:md5,5d2a1f6080254676bff1ef3b27ab641f",
-                "versions.yml:md5,c35cd6c1892a24d29169bc79e7623bd9",
-                "versions.yml:md5,dc55a53ff7729dc648ac35db31ec5656",
-                "versions.yml:md5,faeabb0df6266c84e0fcd7f39d60b759"
-            ]
+            [],
+            []
         ],
         "meta": {
             "nf-test": "0.9.2",
@@ -456,4 +326,4 @@
         },
         "timestamp": "2025-11-03T16:51:00.574162"
     }
-}
\ No newline at end of file
+}
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index d51d2b2728..52553eb5c0 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -22,8 +22,6 @@ workflow PREPARE_INTERVALS {
     step
 
     main:
-    versions = channel.empty()
-
     intervals_bed        = channel.empty() // List of [ bed, num_intervals ], one for each region
     intervals_bed_gz_tbi = channel.empty() // List of [ bed.gz, bed,gz.tbi, num_intervals ], one for each region
     intervals_combined   = channel.empty() // Single bed file containing all intervals
@@ -47,15 +45,12 @@ workflow PREPARE_INTERVALS {
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
 
-            versions = versions.mix(CREATE_INTERVALS_BED.out.versions)
         } else {
             intervals_combined = channel.fromPath(file(intervals)).map{bed -> [ [ id:bed.baseName ], bed ] }
             CREATE_INTERVALS_BED(file(intervals), nucleotides_per_second)
 
             intervals_bed = CREATE_INTERVALS_BED.out.bed
 
-            versions = versions.mix(CREATE_INTERVALS_BED.out.versions)
-
             // If interval file is not provided as .bed, but e.g. as .interval_list then convert to BED format
             if (intervals.endsWith(".interval_list")) {
                 GATK4_INTERVALLISTTOBED(intervals_combined)
@@ -108,6 +103,4 @@ workflow PREPARE_INTERVALS {
     // All intervals in one file
     intervals_bed_combined        // [ intervals.bed ]
     intervals_bed_gz_tbi_combined // [ intervals.bed.gz, intervals.bed.gz.tbi]
-
-    versions               // [ versions.yml ]
 }
diff --git a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
index 0142d6e3bd..cd2d52bdcc 100644
--- a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
@@ -35,8 +35,6 @@ workflow PIPELINE_INITIALISATION {
 
     main:
 
-    versions = channel.empty()
-
     // Print version and exit if required and dump pipeline parameters to JSON file
     UTILS_NEXTFLOW_PIPELINE(
         version,
@@ -190,7 +188,6 @@ workflow PIPELINE_INITIALISATION {
 
     emit:
     samplesheet = SAMPLESHEET_TO_CHANNEL.out.input_sample
-    versions
 }
 
 /*
diff --git a/subworkflows/local/vcf_concatenate_germline/main.nf b/subworkflows/local/vcf_concatenate_germline/main.nf
index db649fdaab..bc517910cb 100644
--- a/subworkflows/local/vcf_concatenate_germline/main.nf
+++ b/subworkflows/local/vcf_concatenate_germline/main.nf
@@ -13,8 +13,6 @@ workflow CONCATENATE_GERMLINE_VCFS {
     vcfs
 
     main:
-    versions = channel.empty()
-
     // Concatenate vcf-files
     ADD_INFO_TO_VCF(vcfs)
     TABIX_EXT_VCF(ADD_INFO_TO_VCF.out.vcf.map{ meta, vcf -> [ meta, vcf, [], [] ] }, 'compress', true, 'vcf')
@@ -25,11 +23,7 @@ workflow CONCATENATE_GERMLINE_VCFS {
     GERMLINE_VCFS_CONCAT(germline_vcfs_with_tbis)
     GERMLINE_VCFS_CONCAT_SORT(GERMLINE_VCFS_CONCAT.out.vcf)
 
-    // Gather versions of all tools used
-    versions = versions.mix(ADD_INFO_TO_VCF.out.versions)
-
     emit:
     vcfs     = GERMLINE_VCFS_CONCAT_SORT.out.vcf // concatenated vcfs
     tbis     = GERMLINE_VCFS_CONCAT_SORT.out.index // matching tbis
-    versions // channel: [ versions.yml ]
 }
diff --git a/subworkflows/local/vcf_normalization/main.nf b/subworkflows/local/vcf_normalization/main.nf
index 4c17cbe412..5b540d5958 100644
--- a/subworkflows/local/vcf_normalization/main.nf
+++ b/subworkflows/local/vcf_normalization/main.nf
@@ -13,8 +13,6 @@ workflow NORMALIZE_VCFS {
     fasta
 
     main:
-    versions = channel.empty()
-
     // Add additional information to VCF files
     ADD_INFO_TO_VCF(vcfs)
 
@@ -27,11 +25,7 @@ workflow NORMALIZE_VCFS {
     // Sort the normalized VCF files
     VCFS_NORM_SORT(VCFS_NORM.out.vcf)
 
-    // Gather versions of all tools used
-    versions = versions.mix(ADD_INFO_TO_VCF.out.versions)
-
     emit:
     vcfs     = VCFS_NORM_SORT.out.vcf   // normalized vcfs
     tbis     = VCFS_NORM_SORT.out.index // matching tbis
-    versions // Channel: [versions.yml]
 }
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 18611e17ef..353a63d0c8 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -124,7 +124,6 @@ workflow SAREK {
     vep_genome
     vep_species
     snpsift_db // channel: [[databases], [tbis], [vardbs], [fields], [prefixes]]
-    versions
 
     main:
     // To gather all QC reports for MultiQC
@@ -223,7 +222,7 @@ workflow SAREK {
             cram_variant_calling = channel.empty()
             cram_variant_calling = cram_variant_calling.mix(FASTQ_PREPROCESS_PARABRICKS.out.cram)
 
-            // Gather used softwares versions
+            // Gather QC reports
             reports = reports.mix(FASTQ_PREPROCESS_PARABRICKS.out.reports)
         }
         else {
@@ -539,11 +538,6 @@ workflow SAREK {
 
         CHANNEL_VARIANT_CALLING_CREATE_CSV(vcf_to_annotate, params.outdir)
 
-        // Gather used variant calling softwares versions
-        versions = versions.mix(BAM_VARIANT_CALLING_GERMLINE_ALL.out.versions)
-        versions = versions.mix(BAM_VARIANT_CALLING_SOMATIC_ALL.out.versions)
-        versions = versions.mix(POST_VARIANTCALLING.out.versions)
-
         // ANNOTATE
         if (step == 'annotate') {
             vcf_to_annotate = input_sample
@@ -579,7 +573,7 @@ workflow SAREK {
     def collated_versions = channel.empty()
     if (!(skip_tools.split(',').contains('versions'))) {
         collated_versions = softwareVersionsToYAML(
-            softwareVersions: versions.mix(channel.topic("versions")),
+            softwareVersions: channel.topic("versions"),
             nextflowVersion: workflow.nextflow.version,
         ).collectFile(
             storeDir: "${params.outdir}/pipeline_info",
@@ -628,7 +622,6 @@ workflow SAREK {
     emit:
     multiqc_report  = MULTIQC.out.report.map { _meta, report -> [report] }.toList() // channel: /path/to/multiqc_report.html
     multiqc_publish = MULTIQC.out.data.mix(MULTIQC.out.plots, MULTIQC.out.report)
-    versions // channel: [ path(versions.yml) ]
 }
 
 /*

From b18bf7b123682fe3e8078ea6e033d4dbec176ac8 Mon Sep 17 00:00:00 2001
From: Gary Burnett <33267967+gburnett-nvidia@users.noreply.github.com>
Date: Wed, 29 Jul 2026 08:23:18 -0400
Subject: [PATCH 21/27] Add Parabricks (GPU-based) Haplotypecaller (#2176)

This PR adds the Parabricks Haplotypecaller as an option for variant
calling.

## PR checklist

- [x] This comment contains a description of changes (with reason).
- [x] If you've fixed a bug or added code that should be tested, add
tests!
- [x] Make sure your code lints (`nf-core pipelines lint`).
- [x] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [x] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [x] Usage Documentation in `docs/usage.md` is updated.
- [x] Output Documentation in `docs/output.md` is updated.
- [x] `CHANGELOG.md` is updated.
- [x] `README.md` is updated (including new tool citations and
authors/contributors).

---------

Co-authored-by: Claude Sonnet 4.6 <noreply@anthropic.com>
Co-authored-by: Maxime U Garcia <max.u.garcia@gmail.com>
Co-authored-by: nf-core-bot <core@nf-co.re>
Co-authored-by: Friederike Hanssen <friederike.hanssen@seqera.io>
---
 CHANGELOG.md                                  |   1 +
 README.md                                     |   1 +
 .../modules/parabricks_haplotypecaller.config |  35 ++
 conf/test.config                              |   8 +
 docs/images/sarek_subway.png                  | Bin 1688402 -> 1413771 bytes
 docs/images/sarek_subway.svg                  |   2 +-
 docs/images/sarek_subway_animated.svg         |   2 +-
 docs/output.md                                |  15 +
 docs/usage.md                                 |  11 +
 modules.json                                  |   5 +
 .../parabricks/haplotypecaller/main.nf        |  64 ++
 .../parabricks/haplotypecaller/meta.yml       | 102 ++++
 nextflow.config                               |   1 +
 nextflow_schema.json                          |   4 +-
 .../bam_variant_calling_germline_all/main.nf  |  67 ++-
 .../main.nf                                   |  40 ++
 .../local/post_variantcalling/main.nf         |   4 +-
 .../local/samplesheet_to_channel/main.nf      |   4 +-
 tests/nextflow.config                         |   9 +
 ...calling_parabricks_haplotypecaller.nf.test |  63 ++
 ...ng_parabricks_haplotypecaller.nf.test.snap | 557 ++++++++++++++++++
 21 files changed, 963 insertions(+), 32 deletions(-)
 create mode 100644 conf/modules/parabricks_haplotypecaller.config
 create mode 100644 modules/nf-core/parabricks/haplotypecaller/main.nf
 create mode 100644 modules/nf-core/parabricks/haplotypecaller/meta.yml
 create mode 100644 subworkflows/local/bam_variant_calling_parabricks_haplotypecaller/main.nf
 create mode 100644 tests/variant_calling_parabricks_haplotypecaller.nf.test
 create mode 100644 tests/variant_calling_parabricks_haplotypecaller.nf.test.snap

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 5e3f69d1b1..115879afa6 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -106,6 +106,7 @@ Sarvesjåhkå is the biggest stream from Sarvesvágge to flow in Rapaätno.
 ### Added
 
 - [#2087](https://github.com/nf-core/sarek/pull/2087) - Add `bam` as output format for parabricks/fq2bam, add multi lane support
+- [#2176](https://github.com/nf-core/sarek/pull/2176) - Add Parabricks HaplotypeCaller as GPU-accelerated germline variant caller (`--tools parabricks_haplotypecaller`)
 - [#2194](https://github.com/nf-core/sarek/pull/2194) - Add `--vep_cache_preflight_check` parameter to force preflight check for local VEP cache download
 - [#2199](https://github.com/nf-core/sarek/pull/2199) - Add animated metro map (`docs/images/sarek_subway_animated.svg`) with dots flowing through the workflow
 
diff --git a/README.md b/README.md
index f73c761058..d5deca451c 100644
--- a/README.md
+++ b/README.md
@@ -57,6 +57,7 @@ Depending on the options and samples provided, the pipeline can currently perfor
   - `freebayes`
   - `GATK HaplotypeCaller`
   - `GATK Mutect2`
+  - _Experimental Feature_: `Parabricks HaplotypeCaller` (GPU-accelerated germline calling, `--tools parabricks_haplotypecaller`)
   - `indexcov`
   - `Lofreq`
   - `Manta`
diff --git a/conf/modules/parabricks_haplotypecaller.config b/conf/modules/parabricks_haplotypecaller.config
new file mode 100644
index 0000000000..ea49e83f06
--- /dev/null
+++ b/conf/modules/parabricks_haplotypecaller.config
@@ -0,0 +1,35 @@
+/*
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Config file for defining DSL2 per module options and publishing paths
+~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+    Available keys to override module options:
+        ext.args   = Additional arguments appended to command in module.
+        ext.args2  = Second set of arguments appended to command in module (multi-tool modules).
+        ext.args3  = Third set of arguments appended to command in module (multi-tool modules).
+        ext.prefix = File name prefix for output files.
+        ext.when   = When to run the module.
+----------------------------------------------------------------------------------------
+*/
+
+// PARABRICKS HAPLOTYPECALLER
+
+process {
+
+    withName: 'PARABRICKS_HAPLOTYPECALLER' {
+        ext.args   = { params.joint_germline ? "--gvcf" : "" }
+        ext.prefix = { params.joint_germline ? "${meta.id}.parabricks_haplotypecaller.g" : "${meta.id}.parabricks_haplotypecaller" }
+        publishDir = [
+            enabled: false
+        ]
+    }
+
+    withName: 'NFCORE_SAREK:SAREK:BAM_VARIANT_CALLING_GERMLINE_ALL:BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER:TABIX_BGZIPTABIX' {
+        ext.prefix = { params.joint_germline ? "${meta.id}.parabricks_haplotypecaller.g" : "${meta.id}.parabricks_haplotypecaller" }
+        publishDir = [
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}/variant_calling/parabricks_haplotypecaller/${meta.id}/" },
+            pattern: "*{vcf.gz,vcf.gz.tbi}"
+        ]
+    }
+
+}
diff --git a/conf/test.config b/conf/test.config
index beffbc64bc..9d0d070105 100644
--- a/conf/test.config
+++ b/conf/test.config
@@ -89,4 +89,12 @@ process {
             "--low-memory",
         ].join(' ').trim() }
     }
+
+    withName: '.*:PARABRICKS_HAPLOTYPECALLER' {
+        // GPU OOM on the g4dn.xlarge CI runners (Tesla T4, 15 GiB VRAM) without this flag
+        ext.args = { [
+            params.joint_germline ? "--gvcf" : "",
+            "--htvc-low-memory",
+        ].join(' ').trim() }
+    }
 }
diff --git a/docs/images/sarek_subway.png b/docs/images/sarek_subway.png
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diff --git a/docs/images/sarek_subway.svg b/docs/images/sarek_subway.svg
index ee526e85d5..bf5afe261f 100644
--- a/docs/images/sarek_subway.svg
+++ b/docs/images/sarek_subway.svg
@@ -3044,7 +3044,7 @@
        cy="-155.61234"
        cx="95.08654"
        id="circle1664-6-38"
-       style="display:inline;fill:#ffffff;fill-rule:evenodd;stroke:#000000;stroke-width:0.999999;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
+       style="display:inline;fill:#24af63;fill-opacity:1;fill-rule:evenodd;stroke:#000000;stroke-width:0.999999;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        transform="scale(1,-1)"
        rx="2.6458333"
        ry="2.645781" /><ellipse
diff --git a/docs/images/sarek_subway_animated.svg b/docs/images/sarek_subway_animated.svg
index 32b5e4486a..1a69b9ba7a 100644
--- a/docs/images/sarek_subway_animated.svg
+++ b/docs/images/sarek_subway_animated.svg
@@ -3044,7 +3044,7 @@
        cy="-155.61234"
        cx="95.08654"
        id="circle1664-6-38"
-       style="display:inline;fill:#ffffff;fill-rule:evenodd;stroke:#000000;stroke-width:0.999999;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
+       style="display:inline;fill:#24af63;fill-opacity:1;fill-rule:evenodd;stroke:#000000;stroke-width:0.999999;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        transform="scale(1,-1)"
        rx="2.6458333"
        ry="2.645781" /><ellipse
diff --git a/docs/output.md b/docs/output.md
index 044090836c..e6eb119d60 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -39,6 +39,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
     - [GATK HaplotypeCaller](#gatk-haplotypecaller)
       - [GATK Germline Single Sample Variant Calling](#gatk-germline-single-sample-variant-calling)
       - [GATK Joint Germline Variant Calling](#gatk-joint-germline-variant-calling)
+    - [Parabricks HaplotypeCaller](#parabricks-haplotypecaller)
     - [GATK Mutect2](#gatk-mutect2)
     - [Lofreq](#lofreq)
     - [MuSE](#muse)
@@ -474,6 +475,20 @@ If the haplotype-called VCF files are not filtered, then Sarek should be run wit
 
 </details>
 
+#### Parabricks HaplotypeCaller
+
+[Parabricks HaplotypeCaller](https://docs.nvidia.com/clara/parabricks/latest/documentation/tooldocs/man_haplotypecaller.html) is a GPU-accelerated implementation of GATK HaplotypeCaller for germline SNP and indel calling. Enable with `--tools parabricks_haplotypecaller --profile <docker/singularity>,gpu`.
+
+<details markdown="1">
+<summary>Output files</summary>
+
+**Output directory: `{outdir}/variant_calling/parabricks_haplotypecaller/<sample>/`**
+
+- `<sample>.parabricks_haplotypecaller.vcf.gz` and `<sample>.parabricks_haplotypecaller.vcf.gz.tbi`
+  - VCF with tabix index
+
+</details>
+
 #### GATK Mutect2
 
 [GATK Mutect2](https://gatk.broadinstitute.org/hc/en-us/articles/5358911630107-Mutect2) calls somatic SNVs and indels via local assembly of haplotypes.
diff --git a/docs/usage.md b/docs/usage.md
index 3748327f43..f63f430385 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -208,6 +208,17 @@ process {
 }
 ```
 
+#### Using GPU accelerated variant calling (`--tools parabricks_haplotypecaller`)
+
+> [!NOTE]
+> This is an experimental addition to the pipeline which requires a GPU and does not support `--profile conda`.
+
+To use NVIDIA Clara Parabricks' GPU-accelerated HaplotypeCaller for germline variant calling, add `--tools parabricks_haplotypecaller --profile <docker/singularity>,gpu` to your run command. This replicates GATK HaplotypeCaller germline SNP and indel calling with GPU acceleration.
+
+Parabricks HaplotypeCaller takes a CRAM file as input and outputs a single VCF per sample (no scatter/gather over intervals). Intervals can be provided via `--intervals` to restrict calling to specific regions.
+
+For more details on available arguments, see the [Parabricks HaplotypeCaller documentation](https://docs.nvidia.com/clara/parabricks/latest/documentation/tooldocs/man_haplotypecaller.html).
+
 ### Start with duplicate marking (`--step markduplicates`)
 
 #### Duplicate Marking
diff --git a/modules.json b/modules.json
index 4c47c23b6c..8ff030d7fb 100644
--- a/modules.json
+++ b/modules.json
@@ -416,6 +416,11 @@
                         "git_sha": "f0b0dc3c3f001b1b7bf814a3c353baecbab17ea1",
                         "installed_by": ["modules"]
                     },
+                    "parabricks/haplotypecaller": {
+                        "branch": "master",
+                        "git_sha": "3e0ec6464725c64d81abe928e15a6750d5fdd272",
+                        "installed_by": ["modules"]
+                    },
                     "rbt/vcfsplit": {
                         "branch": "master",
                         "git_sha": "117bda90be326bcff5cb2286c7988a4caa588b5d",
diff --git a/modules/nf-core/parabricks/haplotypecaller/main.nf b/modules/nf-core/parabricks/haplotypecaller/main.nf
new file mode 100644
index 0000000000..9efff97226
--- /dev/null
+++ b/modules/nf-core/parabricks/haplotypecaller/main.nf
@@ -0,0 +1,64 @@
+process PARABRICKS_HAPLOTYPECALLER {
+    tag "${meta.id}"
+    label 'process_high'
+    label 'process_gpu'
+    // needed by the module to work properly can be removed when fixed upstream - see: https://github.com/nf-core/modules/issues/7226
+    stageInMode 'copy'
+
+    container "nvcr.io/nvidia/clara/clara-parabricks:4.7.1-1"
+
+    input:
+    tuple val(meta), path(input), path(input_index), path(intervals)
+    tuple val(ref_meta), path(fasta)
+
+    output:
+    tuple val(meta), path("*.vcf"),      emit: vcf,                 optional: true
+    tuple val(meta), path("*.g.vcf.gz"), emit: gvcf,                optional: true
+    path "compatible_versions.yml",      emit: compatible_versions, optional: true
+    tuple val("${task.process}"), val('parabricks'), eval("pbrun version | grep -m1 '^pbrun:' | sed 's/^pbrun:[[:space:]]*//'"), topic: versions, emit: versions_parabricks
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    // Exit if running this module with -profile conda / -profile mamba
+    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
+        exit(1, "Parabricks module does not support Conda. Please use Docker / Singularity / Podman instead.")
+    }
+    def args   = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
+    def output_file           = args.contains("--gvcf") ? "${prefix}.g.vcf.gz" : "${prefix}.vcf"
+    def intervals_command     = intervals     ? (intervals instanceof List ? intervals.collect { interval -> "--interval-file ${interval}" }.join(' ') : "--interval-file ${intervals}") : ""
+
+    def num_gpus = task.accelerator ? "--num-gpus ${task.accelerator.request}" : ''
+    """
+    pbrun \\
+        haplotypecaller \\
+        --ref ${fasta} \\
+        --in-bam ${input} \\
+        --out-variants ${output_file} \\
+        ${intervals_command} \\
+        ${num_gpus} \\
+        ${args}
+    """
+
+    stub:
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def output_cmd = args.contains("--gvcf") ? "echo '' | gzip > ${prefix}.g.vcf.gz" : "touch ${prefix}.vcf"
+    """
+    ${output_cmd}
+
+    # Capture the full version output once and store it in a variable
+    pbrun_version_output=\$(pbrun haplotypecaller --version 2>&1)
+
+    # Generate compatible_versions.yml
+    cat <<EOF > compatible_versions.yml
+    "${task.process}":
+        pbrun_version: \$(echo "\$pbrun_version_output" | grep "pbrun:" | awk '{print \$2}')
+        compatible_with:
+        \$(echo "\$pbrun_version_output" | awk '/Compatible With:/,/^---/{ if (\$1 ~ /^[A-Z]/ && \$1 != "Compatible" && \$1 != "---") { printf "  %s: %s\\n", \$1, \$2 } }')
+    EOF
+    """
+}
diff --git a/modules/nf-core/parabricks/haplotypecaller/meta.yml b/modules/nf-core/parabricks/haplotypecaller/meta.yml
new file mode 100644
index 0000000000..a3f0839e72
--- /dev/null
+++ b/modules/nf-core/parabricks/haplotypecaller/meta.yml
@@ -0,0 +1,102 @@
+name: "parabricks_haplotypecaller"
+description: NVIDIA Clara Parabricks GPU-accelerated germline variant calling, replicating
+  GATK haplotypecaller.
+keywords:
+  - variant
+  - vcf
+  - haplotypecaller
+  - germline
+tools:
+  - parabricks:
+      description: "NVIDIA Clara Parabricks GPU-accelerated genomics tools"
+      homepage: https://www.nvidia.com/en-us/clara/genomics/
+      documentation: https://docs.nvidia.com/clara/parabricks/latest/index.html
+      licence: ["custom"]
+      identifier: ""
+
+input:
+  - - meta:
+        type: map
+        description: |
+          Groovy Map containing sample information.
+          [ id:'test']
+    - input:
+        type: file
+        description: bam file for sample to be variant called.
+        pattern: "*.bam"
+        ontologies: []
+    - input_index:
+        type: file
+        description: (Optional) bai index corresponding to input bam file. Only necessary
+          when using intervals.
+        pattern: "*.bai"
+        ontologies: []
+    - intervals:
+        type: file
+        description: file or files containing genomic intervals.
+        pattern: "*.{bed,interval_list,picard,list,intervals}"
+        ontologies: []
+  - - ref_meta:
+        type: map
+        description: |
+          Groovy Map containing reference information.
+          [ id:'test']
+    - fasta:
+        type: file
+        description: reference fasta - must be unzipped.
+        pattern: "*.fasta"
+        ontologies: []
+output:
+  vcf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information.
+            e.g. [ id:'test' ]
+      - "*.vcf":
+          type: file
+          description: variant file.
+          pattern: "*.vcf"
+          ontologies: []
+  gvcf:
+    - - meta:
+          type: map
+          description: |
+            Groovy Map containing sample information.
+            e.g. [ id:'test' ]
+      - "*.g.vcf.gz":
+          type: file
+          description: genomic variant file, gzipped.
+          pattern: "*.g.vcf.gz"
+          ontologies:
+            - edam: http://edamontology.org/format_3989 # GZIP format
+  compatible_versions:
+    - compatible_versions.yml:
+        type: file
+        description: File containing info on compatible CPU-based software versions.
+        pattern: "compatible_versions.yml"
+        ontologies:
+          - edam: http://edamontology.org/format_3750 # YAML
+  versions_parabricks:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - parabricks:
+          type: string
+          description: The tool name
+      - "pbrun version | grep -m1 '^pbrun:' | sed 's/^pbrun:[[:space:]]*//'":
+          type: string
+          description: The command used to generate the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The process the versions were collected from
+      - parabricks:
+          type: string
+          description: The tool name
+      - "pbrun version | grep -m1 '^pbrun:' | sed 's/^pbrun:[[:space:]]*//'":
+          type: string
+          description: The command used to generate the version of the tool
+authors:
+  - "@bsiranosian"
diff --git a/nextflow.config b/nextflow.config
index e29cdd1f5e..58794527b4 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -815,6 +815,7 @@ includeConfig 'conf/modules/controlfreec.config'
 includeConfig 'conf/modules/deepvariant.config'
 includeConfig 'conf/modules/freebayes.config'
 includeConfig 'conf/modules/haplotypecaller.config'
+includeConfig 'conf/modules/parabricks_haplotypecaller.config'
 includeConfig 'conf/modules/indexcov.config'
 includeConfig 'conf/modules/joint_germline.config'
 includeConfig 'conf/modules/manta.config'
diff --git a/nextflow_schema.json b/nextflow_schema.json
index 07493019c4..9e1b475e3d 100644
--- a/nextflow_schema.json
+++ b/nextflow_schema.json
@@ -110,8 +110,8 @@
                     "type": "string",
                     "fa_icon": "fas fa-toolbox",
                     "description": "Tools to use for contamination removal, duplicate marking, variant calling and/or for annotation.",
-                    "help_text": "Multiple tools separated with commas.\n\n**Variant Calling:**\n\nGermline variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: DeepVariant, FreeBayes, GATK HaplotypeCaller, mpileup, Sentieon Haplotyper \n- Structural Variants: indexcov, Manta, TIDDIT\n- Copy-number: CNVKit\n\nTumor-only somatic variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Lofreq, mpileup, Mutect2, Sentieon TNScope, Strelka\n- Structural Variants: Manta, Sentieon TNScope, TIDDIT\n- Copy-number: CNVKit, ControlFREEC\n\nSomatic variant calling can currently only be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Mutect2, Sentieon TNScope, Strelka2 \n- Structural variants: Manta, TIDDIT\n- Copy-Number: ASCAT, CNVKit, Control-FREEC, Sentieon TNScope \n- Microsatellite Instability: MSIsensor2, MSIsensorpro\n\n> **NB** Mutect2 for somatic variant calling cannot be combined with `--no_intervals`\n\n**Annotation:**\n \n- snpEff, VEP, merge (both consecutively), and bcftools annotate (needs `--bcftools_annotation`).\n\n> **NB** As Sarek will use bgzip and tabix to compress and index VCF files annotated, it expects VCF files to be sorted when starting from `--step annotate`.",
-                    "pattern": "^((ascat|bbsplit|bcfann|cnvkit|controlfreec|deepvariant|freebayes|haplotypecaller|indexcov|lofreq|manta|merge|mpileup|msisensor2|msisensorpro|muse|mutect2|ngscheckmate|sentieon_dedup|sentieon_dnascope|sentieon_haplotyper|sentieon_tnscope|snpeff|snpsift|strelka|tiddit|vep|varlociraptor)?,?)*(?<!,)$"
+                    "help_text": "Multiple tools separated with commas.\n\n**Variant Calling:**\n\nGermline variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: DeepVariant, FreeBayes, GATK HaplotypeCaller, mpileup, Sentieon Haplotyper, Parabricks Haplotypecaller \n- Structural Variants: indexcov, Manta, TIDDIT\n- Copy-number: CNVKit\n\nTumor-only somatic variant calling can currently be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Lofreq, mpileup, Mutect2, Sentieon TNScope, Strelka\n- Structural Variants: Manta, Sentieon TNScope, TIDDIT\n- Copy-number: CNVKit, ControlFREEC\n\nSomatic variant calling can currently only be performed with the following variant callers:\n- SNPs/Indels: FreeBayes, Mutect2, Sentieon TNScope, Strelka2 \n- Structural variants: Manta, TIDDIT\n- Copy-Number: ASCAT, CNVKit, Control-FREEC, Sentieon TNScope \n- Microsatellite Instability: MSIsensor2, MSIsensorpro\n\n> **NB** Mutect2 for somatic variant calling cannot be combined with `--no_intervals`\n\n**Annotation:**\n \n- snpEff, VEP, merge (both consecutively), and bcftools annotate (needs `--bcftools_annotation`).\n\n> **NB** As Sarek will use bgzip and tabix to compress and index VCF files annotated, it expects VCF files to be sorted when starting from `--step annotate`.",
+                    "pattern": "^((ascat|bbsplit|bcfann|cnvkit|controlfreec|deepvariant|freebayes|haplotypecaller|indexcov|lofreq|manta|merge|mpileup|msisensor2|msisensorpro|muse|mutect2|ngscheckmate|parabricks_haplotypecaller|sentieon_dedup|sentieon_dnascope|sentieon_haplotyper|sentieon_tnscope|snpeff|snpsift|strelka|tiddit|vep|varlociraptor)?,?)*(?<!,)$"
                 },
                 "skip_tools": {
                     "type": "string",
diff --git a/subworkflows/local/bam_variant_calling_germline_all/main.nf b/subworkflows/local/bam_variant_calling_germline_all/main.nf
index f8278a506d..d68ba83207 100644
--- a/subworkflows/local/bam_variant_calling_germline_all/main.nf
+++ b/subworkflows/local/bam_variant_calling_germline_all/main.nf
@@ -9,6 +9,7 @@ include { BAM_VARIANT_CALLING_DEEPVARIANT
 include { BAM_VARIANT_CALLING_FREEBAYES                                                } from '../bam_variant_calling_freebayes/main'
 include { BAM_VARIANT_CALLING_GERMLINE_MANTA                                           } from '../bam_variant_calling_germline_manta/main'
 include { BAM_VARIANT_CALLING_HAPLOTYPECALLER                                          } from '../bam_variant_calling_haplotypecaller/main'
+include { BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER                               } from '../bam_variant_calling_parabricks_haplotypecaller/main'
 include { BAM_VARIANT_CALLING_INDEXCOV                                                 } from '../bam_variant_calling_indexcov/main'
 include { BAM_VARIANT_CALLING_SENTIEON_DNASCOPE                                        } from '../bam_variant_calling_sentieon_dnascope/main'
 include { BAM_VARIANT_CALLING_SENTIEON_HAPLOTYPER                                      } from '../bam_variant_calling_sentieon_haplotyper/main'
@@ -53,30 +54,32 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
 
     main:
     //TODO: Temporary until the if's can be removed and printing to terminal is prevented with "when" in the modules.config
-    gvcf_sentieon_dnascope       = channel.empty()
-    gvcf_sentieon_haplotyper     = channel.empty()
-    gvcf_tbi_sentieon_dnascope   = channel.empty()
+    gvcf_sentieon_dnascope     = channel.empty()
+    gvcf_sentieon_haplotyper   = channel.empty()
+    gvcf_tbi_sentieon_dnascope = channel.empty()
     gvcf_tbi_sentieon_haplotyper = channel.empty()
 
-    out_indexcov             = channel.empty()
-    vcf_deepvariant          = channel.empty()
-    vcf_freebayes            = channel.empty()
-    vcf_haplotypecaller      = channel.empty()
-    vcf_manta                = channel.empty()
-    vcf_mpileup              = channel.empty()
-    vcf_sentieon_dnascope    = channel.empty()
-    vcf_sentieon_haplotyper  = channel.empty()
-    vcf_strelka              = channel.empty()
-    vcf_tiddit               = channel.empty()
-    tbi_deepvariant          = channel.empty()
-    tbi_freebayes            = channel.empty()
-    tbi_haplotypecaller      = channel.empty()
-    tbi_manta                = channel.empty()
-    tbi_mpileup              = channel.empty()
-    tbi_sentieon_dnascope    = channel.empty()
-    tbi_sentieon_haplotyper  = channel.empty()
-    tbi_strelka              = channel.empty()
-    tbi_tiddit               = channel.empty()
+    out_indexcov                       = channel.empty()
+    vcf_deepvariant                    = channel.empty()
+    vcf_freebayes                      = channel.empty()
+    vcf_haplotypecaller                = channel.empty()
+    vcf_manta                          = channel.empty()
+    vcf_mpileup                        = channel.empty()
+    vcf_parabricks_haplotypecaller     = channel.empty()
+    vcf_sentieon_dnascope              = channel.empty()
+    vcf_sentieon_haplotyper            = channel.empty()
+    vcf_strelka                        = channel.empty()
+    vcf_tiddit                         = channel.empty()
+    tbi_deepvariant                    = channel.empty()
+    tbi_freebayes                      = channel.empty()
+    tbi_haplotypecaller                = channel.empty()
+    tbi_manta                          = channel.empty()
+    tbi_mpileup                        = channel.empty()
+    tbi_parabricks_haplotypecaller     = channel.empty()
+    tbi_sentieon_dnascope              = channel.empty()
+    tbi_sentieon_haplotyper            = channel.empty()
+    tbi_strelka                        = channel.empty()
+    tbi_tiddit                         = channel.empty()
 
     // BCFTOOLS MPILEUP
     if (tools && tools.split(',').contains('mpileup')) {
@@ -187,6 +190,18 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
         }
     }
 
+    // PARABRICKS HAPLOTYPECALLER
+    if (tools && tools.split(',').contains('parabricks_haplotypecaller')) {
+        BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER(
+            cram,
+            fasta,
+            intervals_bed_combined
+        )
+
+        vcf_parabricks_haplotypecaller = BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER.out.vcf
+        tbi_parabricks_haplotypecaller = BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER.out.tbi
+    }
+
     // MANTA
     if (tools && tools.split(',').contains('manta')) {
         BAM_VARIANT_CALLING_GERMLINE_MANTA (
@@ -357,10 +372,11 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     vcf_all = channel.empty().mix(
         vcf_deepvariant,
         vcf_freebayes,
-        vcf_sentieon_dnascope,
         vcf_haplotypecaller,
         vcf_manta,
         vcf_mpileup,
+        vcf_parabricks_haplotypecaller,
+        vcf_sentieon_dnascope,
         vcf_sentieon_haplotyper,
         vcf_strelka,
         vcf_tiddit
@@ -369,10 +385,11 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     tbi_all = channel.empty().mix(
         tbi_deepvariant,
         tbi_freebayes,
-        tbi_sentieon_dnascope,
         tbi_haplotypecaller,
         tbi_manta,
         tbi_mpileup,
+        tbi_parabricks_haplotypecaller,
+        tbi_sentieon_dnascope,
         tbi_sentieon_haplotyper,
         tbi_strelka,
         tbi_tiddit
@@ -390,6 +407,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     vcf_haplotypecaller
     vcf_manta
     vcf_mpileup
+    vcf_parabricks_haplotypecaller
     vcf_strelka
     vcf_sentieon_dnascope
     vcf_sentieon_haplotyper
@@ -400,6 +418,7 @@ workflow BAM_VARIANT_CALLING_GERMLINE_ALL {
     tbi_haplotypecaller
     tbi_manta
     tbi_mpileup
+    tbi_parabricks_haplotypecaller
     tbi_sentieon_dnascope
     tbi_sentieon_haplotyper
     tbi_strelka
diff --git a/subworkflows/local/bam_variant_calling_parabricks_haplotypecaller/main.nf b/subworkflows/local/bam_variant_calling_parabricks_haplotypecaller/main.nf
new file mode 100644
index 0000000000..43c88924ff
--- /dev/null
+++ b/subworkflows/local/bam_variant_calling_parabricks_haplotypecaller/main.nf
@@ -0,0 +1,40 @@
+//
+// PARABRICKS HAPLOTYPECALLER germline variant calling (GPU-accelerated)
+//
+
+include { PARABRICKS_HAPLOTYPECALLER             } from '../../../modules/nf-core/parabricks/haplotypecaller/main'
+include { HTSLIB_BGZIPTABIX as TABIX_BGZIPTABIX  } from '../../../modules/nf-core/htslib/bgziptabix/main'
+
+workflow BAM_VARIANT_CALLING_PARABRICKS_HAPLOTYPECALLER {
+    take:
+    cram                    // channel: [mandatory] [ meta, cram, crai ]
+    fasta                   // channel: [mandatory] [ meta, fasta ]
+    intervals_bed_combined  // channel: [optional]  [] or [ intervals.bed ]
+
+    main:
+    // Combine each sample with the (optional) intervals list
+    // intervals_bed_combined emits [] (no intervals) or [file] (one combined BED)
+    // When no_intervals, the empty list contributes 0 elements to the combined tuple,
+    // so use it.size() to safely extract the optional 4th element.
+    cram_intervals = cram
+        .combine(intervals_bed_combined)
+        .map { it ->
+            def (meta, cram_, crai) = it
+            def intervals_ = it.size() > 3 ? it[3] : []
+            [ meta + [ variantcaller:'parabricks_haplotypecaller' ], cram_, crai, intervals_ ]
+        }
+
+    PARABRICKS_HAPLOTYPECALLER(cram_intervals, fasta)
+
+    // Compress and index the uncompressed VCF output
+    TABIX_BGZIPTABIX(PARABRICKS_HAPLOTYPECALLER.out.vcf.map { meta, vcf_ -> [ meta, vcf_, [], [] ] }, 'compress', true, 'vcf')
+
+    vcf_tbi = TABIX_BGZIPTABIX.out.output.join(TABIX_BGZIPTABIX.out.index)
+
+    vcf = vcf_tbi.map { meta, vcf_, _tbi -> [ meta, vcf_ ] }
+    tbi = vcf_tbi.map { meta, _vcf, tbi  -> [ meta, tbi  ] }
+
+    emit:
+    vcf      // channel: [ val(meta), vcf.gz ]
+    tbi      // channel: [ val(meta), vcf.gz.tbi ]
+}
diff --git a/subworkflows/local/post_variantcalling/main.nf b/subworkflows/local/post_variantcalling/main.nf
index 3dc33dde0c..e230544d9a 100644
--- a/subworkflows/local/post_variantcalling/main.nf
+++ b/subworkflows/local/post_variantcalling/main.nf
@@ -76,8 +76,8 @@ workflow POST_VARIANTCALLING {
         // - manta, tiddit: structural variant callers (separate workflow)
         // - samtools mpileup produces pileup format for ControlFREEC, not consensus-ready VCFs
         def small_variantcallers = ['bcftools', 'deepvariant', 'freebayes', 'haplotypecaller',
-                                    'lofreq', 'muse', 'mutect2', 'sentieon_dnascope',
-                                    'sentieon_haplotyper', 'sentieon_tnscope', 'strelka' ]
+                                    'lofreq', 'muse', 'mutect2', 'parabricks_haplotypecaller',
+                                    'sentieon_dnascope', 'sentieon_haplotyper', 'sentieon_tnscope', 'strelka' ]
 
         def excluded_variantcallers = ['manta', 'tiddit', 'samtools']
 
diff --git a/subworkflows/local/samplesheet_to_channel/main.nf b/subworkflows/local/samplesheet_to_channel/main.nf
index 92e76472be..b6caf445e2 100644
--- a/subworkflows/local/samplesheet_to_channel/main.nf
+++ b/subworkflows/local/samplesheet_to_channel/main.nf
@@ -396,8 +396,8 @@ workflow SAMPLESHEET_TO_CHANNEL {
             log.warn("If GATK's Haplotypecaller, Sentieon's Dnascope or Sentieon's Haplotyper is specified, without `--dbsnp` or `--known_indels no filtering will be done. For filtering, please provide at least one of `--dbsnp` or `--known_indels`.\nFor more information see FilterVariantTranches (single-sample, default): https://gatk.broadinstitute.org/hc/en-us/articles/5358928898971-FilterVariantTranches\nFor more information see VariantRecalibration (--joint_germline): https://gatk.broadinstitute.org/hc/en-us/articles/5358906115227-VariantRecalibrator\nFor more information on GATK Best practice germline variant calling: https://gatk.broadinstitute.org/hc/en-us/articles/360035535932-Germline-short-variant-discovery-SNPs-Indels-")
         }
     }
-    if (joint_germline && (!tools || !(tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope')))) {
-        error("The GATK's Haplotypecaller, Sentieon's Dnascope or Sentieon's Haplotyper should be specified as one of the tools when doing joint germline variant calling.) ")
+    if (joint_germline && (!tools || !(tools.split(',').contains('haplotypecaller') || tools.split(',').contains('sentieon_haplotyper') || tools.split(',').contains('sentieon_dnascope') || tools.split(',').contains('parabricks_haplotypecaller')))) {
+        error("The GATK's Haplotypecaller, Parabricks' Haplotypecaller, Sentieon's Dnascope or Sentieon's Haplotyper should be specified as one of the tools when doing joint germline variant calling.) ")
     }
 
     if (tools && tools.split(',').contains('haplotypecaller') && joint_germline && no_intervals) {
diff --git a/tests/nextflow.config b/tests/nextflow.config
index c4354c6561..54a47091b9 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -16,6 +16,15 @@ process {
         // complains that training data is constant for MQRankSum, ReadPosRankSum, SOR
         ext.args   = { '--annotation QD  --annotation FS --annotation DP --var_type SNP' }
     }
+    withName: 'PARABRICKS_HAPLOTYPECALLER' {
+        accelerator = [request: 1]
+        containerOptions = '--gpus all'
+        // GPU OOM on the g4dn.xlarge CI runners (Tesla T4, 15 GiB VRAM) without --htvc-low-memory
+        ext.args = { [
+            params.joint_germline ? "--gvcf" : "",
+            "--htvc-low-memory",
+        ].join(' ').trim() }
+    }
 }
 
 // NOTE This is how pipeline users will use Sentieon in real world use
diff --git a/tests/variant_calling_parabricks_haplotypecaller.nf.test b/tests/variant_calling_parabricks_haplotypecaller.nf.test
new file mode 100644
index 0000000000..6f4073fd67
--- /dev/null
+++ b/tests/variant_calling_parabricks_haplotypecaller.nf.test
@@ -0,0 +1,63 @@
+def projectDir = new File('.').absolutePath
+def modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
+
+
+nextflow_pipeline {
+
+    name "Test pipeline"
+    script "../main.nf"
+    profile "test,gpu"
+
+    def test_scenario = [
+        [
+            name: "-profile test,gpu --tools parabricks_haplotypecaller",
+            params: [
+                intervals: modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.multi_intervals.bed',
+                input: "${projectDir}/tests/csv/3.0/mapped_single_bam.csv",
+                tools: "parabricks_haplotypecaller",
+                step: "variant_calling"
+            ],
+            gpu: true,
+            no_conda: true
+        ],
+        [
+            name: "-profile test --tools parabricks_haplotypecaller --no_intervals -stub",
+            params: [
+                input: "${projectDir}/tests/csv/3.0/mapped_single_bam.csv",
+                tools: "parabricks_haplotypecaller",
+                step: "variant_calling",
+                no_intervals: true
+            ],
+            stub: true,
+            no_conda: true
+        ],
+        [
+            name: "-profile test,gpu --tools parabricks_haplotypecaller --joint_germline",
+            params: [
+                intervals: modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.multi_intervals.bed',
+                input: "${projectDir}/tests/csv/3.0/mapped_joint_bam.csv",
+                tools: "parabricks_haplotypecaller",
+                step: "variant_calling",
+                joint_germline: true
+            ],
+            gpu: true,
+            no_conda: true
+        ],
+        [
+            name: "-profile test --tools parabricks_haplotypecaller --joint_germline --no_intervals -stub",
+            params: [
+                input: "${projectDir}/tests/csv/3.0/mapped_joint_bam.csv",
+                tools: "parabricks_haplotypecaller",
+                step: "variant_calling",
+                joint_germline: true,
+                no_intervals: true
+            ],
+            stub: true,
+            no_conda: true
+        ]
+    ]
+
+    test_scenario.each { scenario ->
+        test(scenario.name, UTILS.getTest(scenario))
+    }
+}
diff --git a/tests/variant_calling_parabricks_haplotypecaller.nf.test.snap b/tests/variant_calling_parabricks_haplotypecaller.nf.test.snap
new file mode 100644
index 0000000000..285f64a6e9
--- /dev/null
+++ b/tests/variant_calling_parabricks_haplotypecaller.nf.test.snap
@@ -0,0 +1,557 @@
+{
+    "-profile test,gpu --tools parabricks_haplotypecaller --joint_germline": {
+        "content": [
+            10,
+            {
+                "CREATE_INTERVALS_BED": {
+                    "gawk": "5.3.0"
+                },
+                "MOSDEPTH": {
+                    "gzip": "1.14",
+                    "mosdepth": "0.3.14"
+                },
+                "PARABRICKS_HAPLOTYPECALLER": {
+                    "parabricks": "4.7.1-1"
+                },
+                "SAMTOOLS_STATS": {
+                    "samtools": "1.24"
+                },
+                "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                },
+                "TABIX_BGZIPTABIX_INTERVAL_SPLIT": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                }
+            },
+            [
+                "csv",
+                "multiqc",
+                "multiqc/index.json",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_data/llms-full.txt",
+                "multiqc/multiqc_data/mosdepth-coverage-per-contig-single.txt",
+                "multiqc/multiqc_data/mosdepth-cumcoverage-dist-id.txt",
+                "multiqc/multiqc_data/mosdepth_cov_dist.txt",
+                "multiqc/multiqc_data/mosdepth_cumcov_dist.txt",
+                "multiqc/multiqc_data/mosdepth_perchrom.txt",
+                "multiqc/multiqc_data/multiqc.log",
+                "multiqc/multiqc_data/multiqc.parquet",
+                "multiqc/multiqc_data/multiqc_citations.txt",
+                "multiqc/multiqc_data/multiqc_data.json",
+                "multiqc/multiqc_data/multiqc_general_stats.txt",
+                "multiqc/multiqc_data/multiqc_samtools_stats.txt",
+                "multiqc/multiqc_data/multiqc_software_versions.txt",
+                "multiqc/multiqc_data/multiqc_sources.txt",
+                "multiqc/multiqc_data/samtools-stats-dp.txt",
+                "multiqc/multiqc_data/samtools_alignment_plot.txt",
+                "multiqc/multiqc_data/samtools_insert_size.txt",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_plots/pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-coverage-per-contig-single-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-coverage-per-contig-single-pct.pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-cumcoverage-dist-id.pdf",
+                "multiqc/multiqc_plots/pdf/samtools-stats-dp.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_alignment_plot-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_alignment_plot-pct.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_insert_size.pdf",
+                "multiqc/multiqc_plots/png",
+                "multiqc/multiqc_plots/png/mosdepth-coverage-per-contig-single-cnt.png",
+                "multiqc/multiqc_plots/png/mosdepth-coverage-per-contig-single-pct.png",
+                "multiqc/multiqc_plots/png/mosdepth-cumcoverage-dist-id.png",
+                "multiqc/multiqc_plots/png/samtools-stats-dp.png",
+                "multiqc/multiqc_plots/png/samtools_alignment_plot-cnt.png",
+                "multiqc/multiqc_plots/png/samtools_alignment_plot-pct.png",
+                "multiqc/multiqc_plots/png/samtools_insert_size.png",
+                "multiqc/multiqc_plots/svg",
+                "multiqc/multiqc_plots/svg/mosdepth-coverage-per-contig-single-cnt.svg",
+                "multiqc/multiqc_plots/svg/mosdepth-coverage-per-contig-single-pct.svg",
+                "multiqc/multiqc_plots/svg/mosdepth-cumcoverage-dist-id.svg",
+                "multiqc/multiqc_plots/svg/samtools-stats-dp.svg",
+                "multiqc/multiqc_plots/svg/samtools_alignment_plot-cnt.svg",
+                "multiqc/multiqc_plots/svg/samtools_alignment_plot-pct.svg",
+                "multiqc/multiqc_plots/svg/samtools_insert_size.svg",
+                "multiqc/multiqc_report.html",
+                "pipeline_info",
+                "pipeline_info/nf_core_sarek_software_mqc_versions.yml",
+                "reference",
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+                "reports/mosdepth",
+                "reports/mosdepth/testN",
+                "reports/mosdepth/testN/testN.recal.mosdepth.global.dist.txt",
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+                "reports/mosdepth/testN/testN.recal.mosdepth.summary.txt",
+                "reports/mosdepth/testN/testN.recal.regions.bed.gz",
+                "reports/mosdepth/testN/testN.recal.regions.bed.gz.csi",
+                "reports/mosdepth/testT",
+                "reports/mosdepth/testT/testT.recal.mosdepth.global.dist.txt",
+                "reports/mosdepth/testT/testT.recal.mosdepth.region.dist.txt",
+                "reports/mosdepth/testT/testT.recal.mosdepth.summary.txt",
+                "reports/mosdepth/testT/testT.recal.regions.bed.gz",
+                "reports/mosdepth/testT/testT.recal.regions.bed.gz.csi",
+                "reports/samtools",
+                "reports/samtools/testN",
+                "reports/samtools/testN/testN.recal.cram.stats",
+                "reports/samtools/testT",
+                "reports/samtools/testT/testT.recal.cram.stats"
+            ],
+            [
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+                "mosdepth-cumcoverage-dist-id.txt:md5,f0177ed551b6ec930854d0d221904ec0",
+                "mosdepth_perchrom.txt:md5,9cfd38310d4da59c7242b2a7ae6bd651",
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+                "samtools_alignment_plot.txt:md5,563784066de81f3bc8ccf5fbbe82b3d5",
+                "samtools_insert_size.txt:md5,f3dd80e14876d827eb924c6cc888c782",
+                "testN.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
+                "testN.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
+                "testN.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
+                "testN.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
+                "testN.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
+                "testT.recal.mosdepth.global.dist.txt:md5,3106c114529adc4231badeb3bb38b6d1",
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+                "testT.recal.mosdepth.summary.txt:md5,024649a659caff330dfbef4ac3560542",
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+                "testT.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d"
+            ],
+            "No BAM files",
+            "No CRAM files",
+            "No VCF files",
+            "No warnings"
+        ],
+        "timestamp": "2026-07-28T18:54:40.672644879",
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+                "multiqc/multiqc_data/.stub",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_plots/.stub",
+                "multiqc/multiqc_report.html",
+                "no_intervals.bed",
+                "no_intervals.bed.gz",
+                "no_intervals.bed.gz.tbi",
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+                "reference",
+                "reports",
+                "reports/bcftools",
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+                "reports/bcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.bcftools_stats.txt",
+                "reports/mosdepth",
+                "reports/mosdepth/test",
+                "reports/mosdepth/test/test.recal.global.dist.txt",
+                "reports/mosdepth/test/test.recal.per-base.bed.gz",
+                "reports/mosdepth/test/test.recal.per-base.bed.gz.csi",
+                "reports/mosdepth/test/test.recal.per-base.d4",
+                "reports/mosdepth/test/test.recal.quantized.bed.gz",
+                "reports/mosdepth/test/test.recal.quantized.bed.gz.csi",
+                "reports/mosdepth/test/test.recal.region.dist.txt",
+                "reports/mosdepth/test/test.recal.regions.bed.gz",
+                "reports/mosdepth/test/test.recal.regions.bed.gz.csi",
+                "reports/mosdepth/test/test.recal.summary.txt",
+                "reports/mosdepth/test/test.recal.thresholds.bed.gz",
+                "reports/mosdepth/test/test.recal.thresholds.bed.gz.csi",
+                "reports/samtools",
+                "reports/samtools/test",
+                "reports/samtools/test/test.recal.cram.stats",
+                "reports/vcftools",
+                "reports/vcftools/parabricks_haplotypecaller",
+                "reports/vcftools/parabricks_haplotypecaller/test",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.012",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.012.indv",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.012.pos",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.BEAGLE.GL",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.BEAGLE.PL",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.FILTER.summary",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.INFO",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.LROH",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.Tajima.D",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.TsTv",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.TsTv.qual",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.TsTv.summary",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.bcf",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.discordance.matrix",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.indv",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.indv_in_files",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.sites",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.sites_in_files",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.diff.switch",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.frq",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.frq.count",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.gdepth",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.geno.chisq",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.imiss",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.impute.hap",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.impute.hap.indv",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.impute.hap.legend",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.indel.hist",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.interchrom.geno.ld",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.interchrom.hap.ld",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.ldepth",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.ldepth.mean",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.ldhat.locs",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.ldhat.sites",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.list.geno.ld",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.list.hap.ld",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.mendel",
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+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.relatedness",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.relatedness2",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.removed.sites",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.singletons",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.sites.pi",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.snpden",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.tfam",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.tped",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.vcf",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.weir.fst",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.windowed.pi",
+                "variant_calling",
+                "variant_calling/parabricks_haplotypecaller",
+                "variant_calling/parabricks_haplotypecaller/test",
+                "variant_calling/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.vcf.gz",
+                "variant_calling/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.vcf.gz.tbi"
+            ],
+            [
+                "[WARN] WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected. HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, [CONTAINER]`.",
+                "[WARN] WARN: The `file()` function was called with a glob pattern that matched a collection of files -- use `files()` instead."
+            ]
+        ],
+        "timestamp": "2026-07-28T18:58:18.660429436",
+        "meta": {
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+                "TABIX_BGZIPTABIX_INTERVAL_COMBINED": {
+                    "htslib": "1.24",
+                    "xz": "5.8.3"
+                }
+            },
+            [
+                "csv",
+                "multiqc",
+                "multiqc/index.json",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_data/.stub",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_plots/.stub",
+                "multiqc/multiqc_report.html",
+                "no_intervals.bed",
+                "no_intervals.bed.gz",
+                "no_intervals.bed.gz.tbi",
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+                "pipeline_info/nf_core_sarek_software_mqc_versions.yml",
+                "reference",
+                "reports",
+                "reports/mosdepth",
+                "reports/mosdepth/testN",
+                "reports/mosdepth/testN/testN.recal.global.dist.txt",
+                "reports/mosdepth/testN/testN.recal.per-base.bed.gz",
+                "reports/mosdepth/testN/testN.recal.per-base.bed.gz.csi",
+                "reports/mosdepth/testN/testN.recal.per-base.d4",
+                "reports/mosdepth/testN/testN.recal.quantized.bed.gz",
+                "reports/mosdepth/testN/testN.recal.quantized.bed.gz.csi",
+                "reports/mosdepth/testN/testN.recal.region.dist.txt",
+                "reports/mosdepth/testN/testN.recal.regions.bed.gz",
+                "reports/mosdepth/testN/testN.recal.regions.bed.gz.csi",
+                "reports/mosdepth/testN/testN.recal.summary.txt",
+                "reports/mosdepth/testN/testN.recal.thresholds.bed.gz",
+                "reports/mosdepth/testN/testN.recal.thresholds.bed.gz.csi",
+                "reports/mosdepth/testT",
+                "reports/mosdepth/testT/testT.recal.global.dist.txt",
+                "reports/mosdepth/testT/testT.recal.per-base.bed.gz",
+                "reports/mosdepth/testT/testT.recal.per-base.bed.gz.csi",
+                "reports/mosdepth/testT/testT.recal.per-base.d4",
+                "reports/mosdepth/testT/testT.recal.quantized.bed.gz",
+                "reports/mosdepth/testT/testT.recal.quantized.bed.gz.csi",
+                "reports/mosdepth/testT/testT.recal.region.dist.txt",
+                "reports/mosdepth/testT/testT.recal.regions.bed.gz",
+                "reports/mosdepth/testT/testT.recal.regions.bed.gz.csi",
+                "reports/mosdepth/testT/testT.recal.summary.txt",
+                "reports/mosdepth/testT/testT.recal.thresholds.bed.gz",
+                "reports/mosdepth/testT/testT.recal.thresholds.bed.gz.csi",
+                "reports/samtools",
+                "reports/samtools/testN",
+                "reports/samtools/testN/testN.recal.cram.stats",
+                "reports/samtools/testT",
+                "reports/samtools/testT/testT.recal.cram.stats"
+            ],
+            [
+                "[WARN] WARN: nf-core pipelines do not accept positional arguments. The positional argument `true` has been detected. HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, [CONTAINER]`.",
+                "[WARN] WARN: The `file()` function was called with a glob pattern that matched a collection of files -- use `files()` instead."
+            ]
+        ],
+        "timestamp": "2026-07-28T19:00:35.123214477",
+        "meta": {
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+            [
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+                "multiqc",
+                "multiqc/index.json",
+                "multiqc/multiqc_data",
+                "multiqc/multiqc_data/bcftools-stats-subtypes.txt",
+                "multiqc/multiqc_data/bcftools_stats_indel-lengths.txt",
+                "multiqc/multiqc_data/bcftools_stats_variant_depths.txt",
+                "multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt",
+                "multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt",
+                "multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt",
+                "multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt",
+                "multiqc/multiqc_data/llms-full.txt",
+                "multiqc/multiqc_data/mosdepth-coverage-per-contig-single.txt",
+                "multiqc/multiqc_data/mosdepth-cumcoverage-dist-id.txt",
+                "multiqc/multiqc_data/mosdepth_cov_dist.txt",
+                "multiqc/multiqc_data/mosdepth_cumcov_dist.txt",
+                "multiqc/multiqc_data/mosdepth_perchrom.txt",
+                "multiqc/multiqc_data/multiqc.log",
+                "multiqc/multiqc_data/multiqc.parquet",
+                "multiqc/multiqc_data/multiqc_bcftools_stats.txt",
+                "multiqc/multiqc_data/multiqc_citations.txt",
+                "multiqc/multiqc_data/multiqc_data.json",
+                "multiqc/multiqc_data/multiqc_general_stats.txt",
+                "multiqc/multiqc_data/multiqc_samtools_stats.txt",
+                "multiqc/multiqc_data/multiqc_software_versions.txt",
+                "multiqc/multiqc_data/multiqc_sources.txt",
+                "multiqc/multiqc_data/samtools-stats-dp.txt",
+                "multiqc/multiqc_data/samtools_alignment_plot.txt",
+                "multiqc/multiqc_data/samtools_insert_size.txt",
+                "multiqc/multiqc_data/vcftools_tstv_by_count.txt",
+                "multiqc/multiqc_data/vcftools_tstv_by_qual.txt",
+                "multiqc/multiqc_plots",
+                "multiqc/multiqc_plots/pdf",
+                "multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths-log.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf",
+                "multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-coverage-per-contig-single-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-coverage-per-contig-single-pct.pdf",
+                "multiqc/multiqc_plots/pdf/mosdepth-cumcoverage-dist-id.pdf",
+                "multiqc/multiqc_plots/pdf/samtools-stats-dp.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_alignment_plot-cnt.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_alignment_plot-pct.pdf",
+                "multiqc/multiqc_plots/pdf/samtools_insert_size.pdf",
+                "multiqc/multiqc_plots/pdf/vcftools_tstv_by_count.pdf",
+                "multiqc/multiqc_plots/pdf/vcftools_tstv_by_qual.pdf",
+                "multiqc/multiqc_plots/png",
+                "multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png",
+                "multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_indel-lengths-cnt.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_indel-lengths-log.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png",
+                "multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png",
+                "multiqc/multiqc_plots/png/mosdepth-coverage-per-contig-single-cnt.png",
+                "multiqc/multiqc_plots/png/mosdepth-coverage-per-contig-single-pct.png",
+                "multiqc/multiqc_plots/png/mosdepth-cumcoverage-dist-id.png",
+                "multiqc/multiqc_plots/png/samtools-stats-dp.png",
+                "multiqc/multiqc_plots/png/samtools_alignment_plot-cnt.png",
+                "multiqc/multiqc_plots/png/samtools_alignment_plot-pct.png",
+                "multiqc/multiqc_plots/png/samtools_insert_size.png",
+                "multiqc/multiqc_plots/png/vcftools_tstv_by_count.png",
+                "multiqc/multiqc_plots/png/vcftools_tstv_by_qual.png",
+                "multiqc/multiqc_plots/svg",
+                "multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg",
+                "multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths-cnt.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths-log.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg",
+                "multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg",
+                "multiqc/multiqc_plots/svg/mosdepth-coverage-per-contig-single-cnt.svg",
+                "multiqc/multiqc_plots/svg/mosdepth-coverage-per-contig-single-pct.svg",
+                "multiqc/multiqc_plots/svg/mosdepth-cumcoverage-dist-id.svg",
+                "multiqc/multiqc_plots/svg/samtools-stats-dp.svg",
+                "multiqc/multiqc_plots/svg/samtools_alignment_plot-cnt.svg",
+                "multiqc/multiqc_plots/svg/samtools_alignment_plot-pct.svg",
+                "multiqc/multiqc_plots/svg/samtools_insert_size.svg",
+                "multiqc/multiqc_plots/svg/vcftools_tstv_by_count.svg",
+                "multiqc/multiqc_plots/svg/vcftools_tstv_by_qual.svg",
+                "multiqc/multiqc_report.html",
+                "pipeline_info",
+                "pipeline_info/nf_core_sarek_software_mqc_versions.yml",
+                "reference",
+                "reports",
+                "reports/bcftools",
+                "reports/bcftools/parabricks_haplotypecaller",
+                "reports/bcftools/parabricks_haplotypecaller/test",
+                "reports/bcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.bcftools_stats.txt",
+                "reports/mosdepth",
+                "reports/mosdepth/test",
+                "reports/mosdepth/test/test.recal.mosdepth.global.dist.txt",
+                "reports/mosdepth/test/test.recal.mosdepth.region.dist.txt",
+                "reports/mosdepth/test/test.recal.mosdepth.summary.txt",
+                "reports/mosdepth/test/test.recal.regions.bed.gz",
+                "reports/mosdepth/test/test.recal.regions.bed.gz.csi",
+                "reports/samtools",
+                "reports/samtools/test",
+                "reports/samtools/test/test.recal.cram.stats",
+                "reports/vcftools",
+                "reports/vcftools/parabricks_haplotypecaller",
+                "reports/vcftools/parabricks_haplotypecaller/test",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.FILTER.summary",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.TsTv.count",
+                "reports/vcftools/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.TsTv.qual",
+                "variant_calling",
+                "variant_calling/parabricks_haplotypecaller",
+                "variant_calling/parabricks_haplotypecaller/test",
+                "variant_calling/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.vcf.gz",
+                "variant_calling/parabricks_haplotypecaller/test/test.parabricks_haplotypecaller.vcf.gz.tbi"
+            ],
+            [
+                "mosdepth-coverage-per-contig-single.txt:md5,115b9a93bcf511419e6622919172f321",
+                "mosdepth-cumcoverage-dist-id.txt:md5,df2fc825dfc41e30f72f5d125a6447ee",
+                "mosdepth_perchrom.txt:md5,115b9a93bcf511419e6622919172f321",
+                "multiqc_citations.txt:md5,d40980f61eb64026d58102841b7f3860",
+                "samtools-stats-dp.txt:md5,53338147cfd6cffd24eef5c07518fe4c",
+                "samtools_alignment_plot.txt:md5,3622ecb71829cc1c581157e403048fd1",
+                "samtools_insert_size.txt:md5,108caa8155bf293b835c81ae10cc25f4",
+                "test.parabricks_haplotypecaller.bcftools_stats.txt:md5,b4f8cb62900cbf17e8ecda9d66efee58",
+                "test.recal.mosdepth.global.dist.txt:md5,bdb8f185c35dd1eec7ce2f69bce57972",
+                "test.recal.mosdepth.region.dist.txt:md5,f1f1ad86fc280bced1888a5d7d25a3f2",
+                "test.recal.mosdepth.summary.txt:md5,32ea70ef1b99def3dc900b4afd513a40",
+                "test.recal.regions.bed.gz:md5,07bbc084a889f1cece4307fd00214a6e",
+                "test.recal.regions.bed.gz.csi:md5,c984bfd7ccac964973c6c1566992b21d",
+                "test.parabricks_haplotypecaller.FILTER.summary:md5,87a84b5f8ac3d3cbeeef7d60afcdbfe7",
+                "test.parabricks_haplotypecaller.TsTv.count:md5,b77c120ee5cc0423267200c67d60c663"
+            ],
+            "No BAM files",
+            "No CRAM files",
+            [
+                "test.parabricks_haplotypecaller.vcf.gz:md5,aadd1bbde5bfb0b1af58ad9cf8f1c2f7"
+            ],
+            "No warnings"
+        ],
+        "timestamp": "2026-07-28T18:53:14.982219019",
+        "meta": {
+            "nf-test": "0.9.5",
+            "nextflow": "26.04.0"
+        }
+    }
+}
\ No newline at end of file

From 96cbec65b1035596676b26dfcfa75a8a1b4c0767 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Wed, 29 Jul 2026 16:57:43 +0200
Subject: [PATCH 22/27] chore(modules): update 16 outdated nf-core modules
 (#2252)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

Updates all 16 modules flagged by `nf-core pipelines lint` for having a
newer version: `bbmap/bbsplit`, `gatk4/applybqsr`, `parabricks/fq2bam`,
`rbt/vcfsplit`, `sentieon/*` (9 modules),
`snpsift/annmem`+`annmemcreate`, `yte`.

Mostly mechanical container/registry bumps. Two real tool-version bumps:
`parabricks/fq2bam` (4.6.0-1 → 4.7.1-1), `snpsift` (5.4.0a → 5.4.0c).
`gatk4/applybqsr` had a breaking signature change upstream
(fasta/fai/dict merged into one tuple, `ext.suffix` replaced by an
explicit `output_suffix` arg) — fixed in
`subworkflows/local/bam_applybqsr/main.nf`.

**CI follow-ups:**

- 3 snapshots need regenerating: `aligner-parabricks` (GPU runner),
`annotation_snpsift`, `annotation_merge`
- Watch `tests/save_output_as_bam.nf.test` — exercises the
`gatk4/applybqsr` fix; only verified locally via `nextflow run -preview`
(no Docker available here)
- `parabricks/fq2bam` module lint fails on the `nvcr.io` container
prefix — pre-existing nf-core-tools limitation, unrelated to this change

## PR checklist

- [x] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/docs/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [x] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`) — not runnable locally (Docker unavailable); verify
on CI.
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [x] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).

Generated with Claude Code
---
 CHANGELOG.md                                  |  3 +
 conf/modules/recalibrate.config               |  1 +
 modules.json                                  | 32 +++++-----
 modules/nf-core/bbmap/bbsplit/main.nf         |  2 +-
 modules/nf-core/gatk4/applybqsr/main.nf       | 11 ++--
 modules/nf-core/gatk4/applybqsr/meta.yml      | 62 ++++++++++++-------
 modules/nf-core/parabricks/fq2bam/main.nf     |  2 +-
 modules/nf-core/rbt/vcfsplit/main.nf          |  4 +-
 .../nf-core/snpsift/annmem/environment.yml    |  2 +-
 modules/nf-core/snpsift/annmem/main.nf        | 28 +++++----
 .../snpsift/annmemcreate/environment.yml      |  2 +-
 modules/nf-core/snpsift/annmemcreate/main.nf  |  8 +--
 modules/nf-core/yte/main.nf                   | 13 ++--
 modules/nf-core/yte/meta.yml                  |  8 +--
 subworkflows/local/bam_applybqsr/main.nf      | 28 ++++++---
 .../local/fastq_preprocess_gatk/main.nf       |  8 ++-
 tests/aligner-parabricks.nf.test.snap         |  8 +--
 tests/annotation_merge.nf.test.snap           |  4 +-
 tests/annotation_snpsift.nf.test.snap         |  8 +--
 workflows/sarek.nf                            |  1 +
 20 files changed, 131 insertions(+), 104 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index 115879afa6..9d08ccc989 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2242](https://github.com/nf-core/sarek/pull/2242) - Migrate QC/coverage modules (`ascat`, `goleft`, `msisensor2`, `msisensorpro`) to the versions topic channel
 - [#2243](https://github.com/nf-core/sarek/pull/2243) - Migrate `cnvkit/*` modules to the versions topic channel, and replace the deprecated `tabix/tabix`/`tabix/bgziptabix` modules with `htslib/bgziptabix`
 - [#2244](https://github.com/nf-core/sarek/pull/2244) - Migrate local modules (`add_info_to_vcf`, `create_intervals_bed`, `samtools/reindex_bam`) to the versions topic channel
+- [#2252](https://github.com/nf-core/sarek/pull/2252) - Update `bbmap/bbsplit`, `gatk4/applybqsr`, `parabricks/fq2bam` (4.6.0 → 4.7.1), `rbt/vcfsplit`, `sentieon/*`, `snpsift/annmem`+`annmemcreate` (5.4.0a → 5.4.0c), and `yte` modules to their latest revisions. Side effect: `GATK4_APPLYBQSR`'s bam/cram output format is now set directly via a `params.save_output_as_bam` process argument rather than `ext.suffix`, so a custom `modules.config` override of `ext.suffix` on `GATK4_APPLYBQSR` no longer has any effect; `GATK4SPARK_APPLYBQSR` is unaffected and still reads `ext.suffix`.
 
 ### Fixed
 
@@ -57,6 +58,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | mosdepth      | 0.3.10      | 0.3.14      |
 | cnvkit        | 0.9.11      | 0.9.12      |
 | xz            | -           | 5.8.3       |
+| parabricks    | 4.6.0-1     | 4.7.1-1     |
+| snpsift       | 5.4.0a      | 5.4.0c      |
 
 ### Dependencies - plugins
 
diff --git a/conf/modules/recalibrate.config b/conf/modules/recalibrate.config
index 9342cc2e9c..dc10e1f5b1 100644
--- a/conf/modules/recalibrate.config
+++ b/conf/modules/recalibrate.config
@@ -20,6 +20,7 @@ process {
         ext.prefix       = {
             meta.num_intervals <= 1 ? "${meta.id}.recal" : "${meta.id}_${intervals.baseName}.recal"
         }
+        // only consumed by GATK4SPARK_APPLYBQSR — GATK4_APPLYBQSR takes output_suffix as a process argument instead
         ext.suffix       = { params.save_output_as_bam ? 'bam' : 'cram'}
         publishDir       = [[
             mode: params.publish_dir_mode,
diff --git a/modules.json b/modules.json
index 8ff030d7fb..2c414088c3 100644
--- a/modules.json
+++ b/modules.json
@@ -12,7 +12,7 @@
                     },
                     "bbmap/bbsplit": {
                         "branch": "master",
-                        "git_sha": "6d1d4b987af3ee5852d852abf99f8883028cea9c",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "bcftools/annotate": {
@@ -208,7 +208,7 @@
                     },
                     "gatk4/applybqsr": {
                         "branch": "master",
-                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
+                        "git_sha": "ad964832ffcb72516a0a9c2c34688e3443655b5a",
                         "installed_by": ["modules"]
                     },
                     "gatk4/applyvqsr": {
@@ -413,7 +413,7 @@
                     },
                     "parabricks/fq2bam": {
                         "branch": "master",
-                        "git_sha": "f0b0dc3c3f001b1b7bf814a3c353baecbab17ea1",
+                        "git_sha": "3e0ec6464725c64d81abe928e15a6750d5fdd272",
                         "installed_by": ["modules"]
                     },
                     "parabricks/haplotypecaller": {
@@ -423,7 +423,7 @@
                     },
                     "rbt/vcfsplit": {
                         "branch": "master",
-                        "git_sha": "117bda90be326bcff5cb2286c7988a4caa588b5d",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "samtools/bam2fq": {
@@ -473,47 +473,47 @@
                     },
                     "sentieon/applyvarcal": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/bwamem": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dedup": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dnamodelapply": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dnascope": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/gvcftyper": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "19430b872a6e970b62e625d78a29a9b275d9dc18",
                         "installed_by": ["modules"]
                     },
                     "sentieon/haplotyper": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/tnscope": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "sentieon/varcal": {
                         "branch": "master",
-                        "git_sha": "7ad1622c68ea87dd3a16bcc4b82cd5d5878fffd9",
+                        "git_sha": "8ee2033cd73a14f4496e690196dac45426e9cadc",
                         "installed_by": ["modules"]
                     },
                     "snpeff/download": {
@@ -528,12 +528,12 @@
                     },
                     "snpsift/annmem": {
                         "branch": "master",
-                        "git_sha": "e12534f75a4504b67984412122698c1cf1966e6f",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "snpsift/annmemcreate": {
                         "branch": "master",
-                        "git_sha": "e12534f75a4504b67984412122698c1cf1966e6f",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     },
                     "spring/decompress": {
@@ -603,7 +603,7 @@
                     },
                     "yte": {
                         "branch": "master",
-                        "git_sha": "c734c101f0010ec13ed9bbf578477962c32f9cc3",
+                        "git_sha": "6d46786420b4d7bc88eba026eb389c0c5535d120",
                         "installed_by": ["modules"]
                     }
                 }
diff --git a/modules/nf-core/bbmap/bbsplit/main.nf b/modules/nf-core/bbmap/bbsplit/main.nf
index bce2208321..6ba944c593 100644
--- a/modules/nf-core/bbmap/bbsplit/main.nf
+++ b/modules/nf-core/bbmap/bbsplit/main.nf
@@ -4,7 +4,7 @@ process BBMAP_BBSPLIT {
     label 'error_retry'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+    container "${ workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container ?
         'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/5a/5aae5977ff9de3e01ff962dc495bfa23f4304c676446b5fdf2de5c7edfa2dc4e/data' :
         'community.wave.seqera.io/library/bbmap_pigz:07416fe99b090fa9' }"
 
diff --git a/modules/nf-core/gatk4/applybqsr/main.nf b/modules/nf-core/gatk4/applybqsr/main.nf
index 4829d563cb..ac3c56a573 100644
--- a/modules/nf-core/gatk4/applybqsr/main.nf
+++ b/modules/nf-core/gatk4/applybqsr/main.nf
@@ -9,9 +9,8 @@ process GATK4_APPLYBQSR {
 
     input:
     tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals)
-    path fasta
-    path fai
-    path dict
+    tuple val(meta2), path(fasta), path(fai), path(dict)
+    val(output_suffix)
 
     output:
     tuple val(meta), path("${prefix}.bam"), emit: bam, optional: true
@@ -26,7 +25,8 @@ process GATK4_APPLYBQSR {
     def args = task.ext.args ?: ''
     prefix = task.ext.prefix ?: "${meta.id}"
     // suffix can only be bam or cram, cram being the sensible default
-    def suffix = task.ext.suffix && task.ext.suffix == "bam" ? "bam" : "cram"
+    def suffix = output_suffix == "bam" ? "bam" : "cram"
+
     def interval_command = intervals ? "--intervals ${intervals}" : ""
 
     def avail_mem = 3072
@@ -50,7 +50,8 @@ process GATK4_APPLYBQSR {
 
     stub:
     prefix = task.ext.prefix ?: "${meta.id}"
-    def suffix = task.ext.suffix ?: "cram"
+    def suffix = output_suffix == "bam" ? "bam" : "cram"
+
     """
     touch ${prefix}.${suffix}
     if [[ ${suffix} == cram ]]; then
diff --git a/modules/nf-core/gatk4/applybqsr/meta.yml b/modules/nf-core/gatk4/applybqsr/meta.yml
index 3f926ce1c6..5f2e0c20d3 100644
--- a/modules/nf-core/gatk4/applybqsr/meta.yml
+++ b/modules/nf-core/gatk4/applybqsr/meta.yml
@@ -15,7 +15,8 @@ tools:
       homepage: https://gatk.broadinstitute.org/hc/en-us
       documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s
       doi: 10.1158/1538-7445.AM2017-3590
-      licence: ["Apache-2.0"]
+      licence:
+        - "Apache-2.0"
       identifier: ""
 input:
   - - meta:
@@ -27,35 +28,47 @@ input:
         type: file
         description: BAM/CRAM file from alignment
         pattern: "*.{bam,cram}"
-        ontologies: []
+        ontologies:
+          - edam: "http://edamontology.org/format_2572" #BAM
+          - edam: "http://edamontology.org/format_3462" #CRAM
     - input_index:
         type: file
         description: BAI/CRAI file from alignment
         pattern: "*.{bai,crai}"
-        ontologies: []
+        ontologies:
+          - edam: "http://edamontology.org/format_3327" #BAI
     - bqsr_table:
         type: file
         description: Recalibration table from gatk4_baserecalibrator
-        ontologies: []
     - intervals:
         type: file
         description: Bed file with the genomic regions included in the library (optional)
-        ontologies: []
-  - fasta:
-      type: file
-      description: The reference fasta file
-      pattern: "*.fasta"
-      ontologies: []
-  - fai:
-      type: file
-      description: Index of reference fasta file
-      pattern: "*.fasta.fai"
-      ontologies: []
-  - dict:
-      type: file
-      description: GATK sequence dictionary
-      pattern: "*.dict"
-      ontologies: []
+        ontologies:
+          - edam: "http://edamontology.org/format_3003" #BED
+  - - meta2:
+        type: map
+        description: |
+          Groovy Map containing sample information about the reference
+          e.g. [ id:'genome' ]
+    - fasta:
+        type: file
+        description: The reference fasta file
+        pattern: "*.fasta"
+        ontologies:
+          - edam: "http://edamontology.org/format_1929" #FASTA
+    - fai:
+        type: file
+        description: Index of reference fasta file
+        pattern: "*.fasta.fai"
+        ontologies:
+          - edam: "http://edamontology.org/data_3210"
+    - dict:
+        type: file
+        description: GATK sequence dictionary
+        pattern: "*.dict"
+  - output_suffix:
+      type: string
+      description: Output file format, either "bam" or "cram" (cram is the default)
 output:
   bam:
     - - meta:
@@ -67,7 +80,8 @@ output:
           type: file
           description: Recalibrated BAM file
           pattern: "${prefix}.bam"
-          ontologies: []
+          ontologies:
+            - edam: "http://edamontology.org/format_2572" #BAM
   bai:
     - - meta:
           type: map
@@ -78,7 +92,8 @@ output:
           type: file
           description: Recalibrated BAM index file
           pattern: "${prefix}*bai"
-          ontologies: []
+          ontologies:
+            - edam: "http://edamontology.org/format_3327" #BAI
   cram:
     - - meta:
           type: map
@@ -89,7 +104,8 @@ output:
           type: file
           description: Recalibrated CRAM file
           pattern: "${prefix}.cram"
-          ontologies: []
+          ontologies:
+            - edam: "http://edamontology.org/format_3462" #CRAM"
   versions_gatk4:
     - - ${task.process}:
           type: string
diff --git a/modules/nf-core/parabricks/fq2bam/main.nf b/modules/nf-core/parabricks/fq2bam/main.nf
index 5f78b4dbde..11ed6f5361 100644
--- a/modules/nf-core/parabricks/fq2bam/main.nf
+++ b/modules/nf-core/parabricks/fq2bam/main.nf
@@ -5,7 +5,7 @@ process PARABRICKS_FQ2BAM {
     // needed by the module to run on a cluster because we need to copy the fasta reference, see https://github.com/nf-core/modules/issues/9230
     stageInMode 'copy'
 
-    container "nvcr.io/nvidia/clara/clara-parabricks:4.6.0-1"
+    container "nvcr.io/nvidia/clara/clara-parabricks:4.7.1-1"
 
     input:
     tuple val(meta), path(reads)
diff --git a/modules/nf-core/rbt/vcfsplit/main.nf b/modules/nf-core/rbt/vcfsplit/main.nf
index 24e85cd5cb..ef9b182606 100644
--- a/modules/nf-core/rbt/vcfsplit/main.nf
+++ b/modules/nf-core/rbt/vcfsplit/main.nf
@@ -3,9 +3,9 @@ process RBT_VCFSPLIT {
     label 'process_single'
 
     conda "${moduleDir}/environment.yml"
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://depot.galaxyproject.org/singularity/rust-bio-tools:0.42.2--h4458251_1'
-        : 'biocontainers/rust-bio-tools:0.42.2--h4458251_1'}"
+        : 'quay.io/biocontainers/rust-bio-tools:0.42.2--h4458251_1'}"
 
     input:
     tuple val(meta), path(vcf)
diff --git a/modules/nf-core/snpsift/annmem/environment.yml b/modules/nf-core/snpsift/annmem/environment.yml
index c252c50c1b..b3ea5e77d1 100644
--- a/modules/nf-core/snpsift/annmem/environment.yml
+++ b/modules/nf-core/snpsift/annmem/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
 dependencies:
   - bioconda::htslib=1.23
-  - bioconda::snpsift=5.4.0a
+  - bioconda::snpsift=5.4.0c
diff --git a/modules/nf-core/snpsift/annmem/main.nf b/modules/nf-core/snpsift/annmem/main.nf
index c852b39a89..bfd0216961 100644
--- a/modules/nf-core/snpsift/annmem/main.nf
+++ b/modules/nf-core/snpsift/annmem/main.nf
@@ -3,16 +3,16 @@ process SNPSIFT_ANNMEM {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/a1/a116bb44e388ca83fea78d82fe8bdfd5cf3557254e2ec7dd3f1f17354880638c/data' :
-        'community.wave.seqera.io/library/htslib_snpsift:ace461dff1cfc121' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c5/c5ee8f24fb1bcd6e98ecb91fcf013f35a8db193dc9af4883c468e623aaebd7cf/data'
+        : 'community.wave.seqera.io/library/htslib_snpsift:6167cd8f20036c55'}"
 
     input:
     tuple val(meta), path(vcf), path(vcf_tbi)
     tuple path(db_vcf), path(db_vcf_tbi), path(db_vardb), val(db_fields), val(db_prefixes)
 
     output:
-    tuple val(meta), path("*.vcf.gz")    , emit: vcf
+    tuple val(meta), path("*.vcf.gz"), emit: vcf
     tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi
     tuple val("${task.process}"), val('snpsift'), eval("SnpSift -version 2>&1 | grep -oE '[0-9]+\\.[0-9]+[a-z]?'"), emit: versions_snpsift, topic: versions
 
@@ -20,20 +20,22 @@ process SNPSIFT_ANNMEM {
     task.ext.when == null || task.ext.when
 
     script:
-    def args     = task.ext.args ?: ''
-    def prefix   = task.ext.prefix ?: "${meta.id}"
-    def dbs      = db_vcf instanceof List ? db_vcf : [db_vcf]
+    def args = task.ext.args ?: ''
+    def prefix = task.ext.prefix ?: "${meta.id}"
+    def dbs = db_vcf instanceof List ? db_vcf : [db_vcf]
     def all_fields = db_fields instanceof List ? db_fields : [db_fields]
     def prefixes = db_prefixes instanceof List ? db_prefixes : [db_prefixes]
 
     // db_vardb is staged as input so it's present in the work directory;
     // SnpSift finds it automatically next to the VCF as {vcf}.snpsift.vardb/
-    def dbfile_args = dbs.withIndex().collect { db, i ->
-        def f = all_fields[i]
-        def fields = f instanceof List ? f.join(',') : f
-        def p = prefixes[i]
-        "-dbfile ${db}${fields ? " -fields ${fields}" : ''}${p ? " -prefix ${p}" : ''}"
-    }
+    def dbfile_args = dbs
+        .withIndex()
+        .collect { db, i ->
+            def f = all_fields[i]
+            def fields = f instanceof List ? f.join(',') : f
+            def p = prefixes[i]
+            "-dbfile ${db}${fields ? " -fields ${fields}" : ''}${p ? " -prefix ${p}" : ''}"
+        }
 
     """
     SnpSift \\
diff --git a/modules/nf-core/snpsift/annmemcreate/environment.yml b/modules/nf-core/snpsift/annmemcreate/environment.yml
index c252c50c1b..b3ea5e77d1 100644
--- a/modules/nf-core/snpsift/annmemcreate/environment.yml
+++ b/modules/nf-core/snpsift/annmemcreate/environment.yml
@@ -4,4 +4,4 @@ channels:
   - bioconda
 dependencies:
   - bioconda::htslib=1.23
-  - bioconda::snpsift=5.4.0a
+  - bioconda::snpsift=5.4.0c
diff --git a/modules/nf-core/snpsift/annmemcreate/main.nf b/modules/nf-core/snpsift/annmemcreate/main.nf
index afe45b8dc6..73161ad96c 100644
--- a/modules/nf-core/snpsift/annmemcreate/main.nf
+++ b/modules/nf-core/snpsift/annmemcreate/main.nf
@@ -3,9 +3,9 @@ process SNPSIFT_ANNMEMCREATE {
     label 'process_medium'
 
     conda "${moduleDir}/environment.yml"
-    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
-        'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/a1/a116bb44e388ca83fea78d82fe8bdfd5cf3557254e2ec7dd3f1f17354880638c/data' :
-        'community.wave.seqera.io/library/htslib_snpsift:ace461dff1cfc121' }"
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
+        ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c5/c5ee8f24fb1bcd6e98ecb91fcf013f35a8db193dc9af4883c468e623aaebd7cf/data'
+        : 'community.wave.seqera.io/library/htslib_snpsift:6167cd8f20036c55'}"
 
     input:
     tuple val(meta), path(db_vcf), path(db_vcf_tbi), val(db_fields)
@@ -18,7 +18,7 @@ process SNPSIFT_ANNMEMCREATE {
     task.ext.when == null || task.ext.when
 
     script:
-    def args   = task.ext.args ?: ''
+    def args = task.ext.args ?: ''
     def fields = db_fields instanceof List ? db_fields.join(',') : db_fields
 
     """
diff --git a/modules/nf-core/yte/main.nf b/modules/nf-core/yte/main.nf
index c0ea25fe1f..5e886f1e22 100644
--- a/modules/nf-core/yte/main.nf
+++ b/modules/nf-core/yte/main.nf
@@ -5,7 +5,7 @@ process YTE {
     // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
     conda "${moduleDir}/environment.yml"
 
-    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+    container "${workflow.containerEngine in ['singularity', 'apptainer'] && !task.ext.singularity_pull_docker_container
         ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/a4/a427620cd214cbc7f48c8d43fa818be59c6c4ea4a622331d96a8b063a05335b0/data'
         : 'community.wave.seqera.io/library/yte:1.9.4--2a362f82cd32b54a'}"
 
@@ -14,27 +14,24 @@ process YTE {
 
     output:
     tuple val(meta), path("*.yaml"), emit: rendered
-    tuple val("${task.process}"), val('yte'), eval("echo $VERSION"), topic: versions, emit: versions_yte
+    // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    tuple val("${task.process}"), val('yte'), val("1.9.4"), topic: versions, emit: versions_yte
 
     when:
     task.ext.when == null || task.ext.when
 
     script:
-    // No args because tool does not accept args, only stdin/stdout
+    def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
 
     // Use map_file if provided, otherwise use map to create key=value pairs for mapping command
     def mapping_cmd = map_file ? "--variable-file ${map_file}" : "--variables " + map.collect { k, v -> "${k}=${v}" }.join(' ')
-    VERSION = "1.9.4"
-    // WARN: Version information not provided by tool on CLI. Please update this string when bumping
     """
-    yte ${mapping_cmd} < ${template} > ${prefix}.yaml
+    yte ${mapping_cmd} ${args} < ${template} > ${prefix}.yaml
     """
 
     stub:
     def prefix = task.ext.prefix ?: "${meta.id}"
-    VERSION = "1.9.4"
-    // WARN: Version information not provided by tool on CLI. Please update this string when bumping
     """
     touch ${prefix}.yaml
     """
diff --git a/modules/nf-core/yte/meta.yml b/modules/nf-core/yte/meta.yml
index a8350a1e6e..5fd9440d76 100644
--- a/modules/nf-core/yte/meta.yml
+++ b/modules/nf-core/yte/meta.yml
@@ -12,7 +12,6 @@ tools:
       tool_dev_url: "https://github.com/yte-template-engine/yte"
       licence: ["MIT"]
       identifier: ""
-
 input:
   - - meta:
         type: map
@@ -36,7 +35,6 @@ input:
         description: |
           Groovy Map containing mapping information to be used in the template
           e.g. `[ key: value ]` with key being a wildcard in the template
-
 output:
   rendered:
     - - meta:
@@ -55,10 +53,9 @@ output:
       - yte:
           type: string
           description: The tool name
-      - echo $VERSION:
+      - 1.9.4:
           type: eval
           description: The expression to obtain the version of the tool
-
 topics:
   versions:
     - - ${task.process}:
@@ -67,10 +64,9 @@ topics:
       - yte:
           type: string
           description: The tool name
-      - echo $VERSION:
+      - 1.9.4:
           type: eval
           description: The expression to obtain the version of the tool
-
 authors:
   - "@famosab"
 maintainers:
diff --git a/subworkflows/local/bam_applybqsr/main.nf b/subworkflows/local/bam_applybqsr/main.nf
index a573c0393d..d40cf4f5e3 100644
--- a/subworkflows/local/bam_applybqsr/main.nf
+++ b/subworkflows/local/bam_applybqsr/main.nf
@@ -10,11 +10,12 @@ include { GATK4_APPLYBQSR           } from '../../../modules/nf-core/gatk4/apply
 
 workflow BAM_APPLYBQSR {
     take:
-    cram      // channel: [mandatory] [ meta, cram, crai, recal ]
-    dict      // channel: [mandatory] [ meta, dict ]
-    fasta     // channel: [mandatory] [ meta, fasta ]
-    fasta_fai // channel: [mandatory] [ meta, fasta_fai ]
-    intervals // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
+    cram                // channel: [mandatory] [ meta, cram, crai, recal ]
+    dict                // channel: [mandatory] [ meta, dict ]
+    fasta               // channel: [mandatory] [ meta, fasta ]
+    fasta_fai           // channel: [mandatory] [ meta, fasta_fai ]
+    intervals           // channel: [mandatory] [ intervals, num_intervals ] or [ [], 0 ] if no intervals
+    save_output_as_bam  // boolean: [mandatory] params.save_output_as_bam
 
     main:
 
@@ -24,22 +25,29 @@ workflow BAM_APPLYBQSR {
         .combine(intervals)
         .map { meta, cram_, crai, recal, intervals_, num_intervals -> [meta + [num_intervals: num_intervals], cram_, crai, recal, intervals_] }
 
+    // fasta/fasta_fai/dict carry independent meta maps (computed vs user-supplied) so they
+    // can't be joined by key — reshape into one tuple the way CRAM_SAMPLEQC does in sarek.nf
+    fasta_fai_dict = fasta
+        .combine(fasta_fai)
+        .combine(dict)
+        .map { meta_fasta, fasta_, _meta_fai, fai, _meta_dict, dict_ -> [meta_fasta, fasta_, fai, dict_] }
+        .collect()
+
     // RUN APPLYBQSR
     GATK4_APPLYBQSR(
         cram_intervals,
-        fasta.map { _meta, fasta_ -> [fasta_] },
-        fasta_fai.map { _meta, fasta_fai_ -> [fasta_fai_] },
-        dict.map { _meta, dict_ -> [dict_] },
+        fasta_fai_dict,
+        save_output_as_bam ? 'bam' : 'cram',
     )
 
-    // BAM path — populated when ext.suffix='bam', empty otherwise
+    // BAM path — populated when save_output_as_bam is true, empty otherwise
     bam_to_merge = GATK4_APPLYBQSR.out.bam
         .map { meta, bam_ -> [groupKey(meta, meta.num_intervals), bam_] }
         .groupTuple()
 
     BAM_MERGE_INDEX_SAMTOOLS(bam_to_merge)
 
-    // CRAM path — populated when ext.suffix='cram', empty otherwise
+    // CRAM path — populated when save_output_as_bam is false, empty otherwise
     cram_to_merge = GATK4_APPLYBQSR.out.cram.map { meta, cram_ -> [groupKey(meta, meta.num_intervals), cram_] }.groupTuple()
 
     CRAM_MERGE_INDEX_SAMTOOLS(
diff --git a/subworkflows/local/fastq_preprocess_gatk/main.nf b/subworkflows/local/fastq_preprocess_gatk/main.nf
index 044dd0173e..50bc8ce660 100644
--- a/subworkflows/local/fastq_preprocess_gatk/main.nf
+++ b/subworkflows/local/fastq_preprocess_gatk/main.nf
@@ -58,6 +58,7 @@ workflow FASTQ_PREPROCESS_GATK {
         known_sites_indels
         known_sites_indels_tbi
         bbsplit_index
+        save_output_as_bam
 
     main:
 
@@ -231,8 +232,8 @@ workflow FASTQ_PREPROCESS_GATK {
 
             BAM_TO_CRAM_MAPPING(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, fasta.combine(fasta_fai).map { meta, fasta_, _meta_fai, fai -> [ meta, fasta_, fai ] }.collect())
             // Create CSV to restart from this step
-            if (params.save_output_as_bam) CHANNEL_ALIGN_CREATE_CSV(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, params.outdir, params.save_output_as_bam)
-            else CHANNEL_ALIGN_CREATE_CSV(BAM_TO_CRAM_MAPPING.out.cram.join(BAM_TO_CRAM_MAPPING.out.crai, failOnDuplicate: true, failOnMismatch: true), params.outdir, params.save_output_as_bam)
+            if (save_output_as_bam) CHANNEL_ALIGN_CREATE_CSV(BAM_MERGE_INDEX_SAMTOOLS.out.bam_bai, params.outdir, save_output_as_bam)
+            else CHANNEL_ALIGN_CREATE_CSV(BAM_TO_CRAM_MAPPING.out.cram.join(BAM_TO_CRAM_MAPPING.out.crai, failOnDuplicate: true, failOnMismatch: true), params.outdir, save_output_as_bam)
         }
 
     }
@@ -437,7 +438,8 @@ workflow FASTQ_PREPROCESS_GATK {
                     dict,
                     fasta,
                     fasta_fai,
-                    intervals_and_num_intervals)
+                    intervals_and_num_intervals,
+                    save_output_as_bam)
 
                 cram_variant_calling_no_spark = BAM_APPLYBQSR.out.alignment
             }
diff --git a/tests/aligner-parabricks.nf.test.snap b/tests/aligner-parabricks.nf.test.snap
index ff3cb07b10..d75c339726 100644
--- a/tests/aligner-parabricks.nf.test.snap
+++ b/tests/aligner-parabricks.nf.test.snap
@@ -29,7 +29,7 @@
                     "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
-                    "parabricks": "4.6.0-1"
+                    "parabricks": "4.7.1-1"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": "1.24"
@@ -260,7 +260,7 @@
                     "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
-                    "parabricks": "4.6.0-1"
+                    "parabricks": "4.7.1-1"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": "1.24"
@@ -515,7 +515,7 @@
                     "gatk4": "4.6.2.0"
                 },
                 "PARABRICKS_FQ2BAM": {
-                    "parabricks": "4.6.0-1"
+                    "parabricks": "4.7.1-1"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": "1.24"
@@ -825,7 +825,7 @@
                     "mosdepth": "0.3.14"
                 },
                 "PARABRICKS_FQ2BAM": {
-                    "parabricks": "4.6.0-1"
+                    "parabricks": "4.7.1-1"
                 },
                 "SAMTOOLS_STATS": {
                     "samtools": "1.24"
diff --git a/tests/annotation_merge.nf.test.snap b/tests/annotation_merge.nf.test.snap
index fe4b81e087..6bfd34e88d 100644
--- a/tests/annotation_merge.nf.test.snap
+++ b/tests/annotation_merge.nf.test.snap
@@ -127,10 +127,10 @@
                     "snpeff": "5.4c"
                 },
                 "SNPSIFT_ANNMEM": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 },
                 "SNPSIFT_ANNMEMCREATE": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 },
                 "VCF_ANNOTATE_MERGE": {
                     "ensemblvep": "116.0",
diff --git a/tests/annotation_snpsift.nf.test.snap b/tests/annotation_snpsift.nf.test.snap
index 853d9ce04d..f3b5923af6 100644
--- a/tests/annotation_snpsift.nf.test.snap
+++ b/tests/annotation_snpsift.nf.test.snap
@@ -4,10 +4,10 @@
             3,
             {
                 "SNPSIFT_ANNMEM": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 },
                 "SNPSIFT_ANNMEMCREATE": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 }
             },
             [
@@ -53,10 +53,10 @@
             3,
             {
                 "SNPSIFT_ANNMEM": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 },
                 "SNPSIFT_ANNMEMCREATE": {
-                    "snpsift": "5.4a"
+                    "snpsift": "5.4c"
                 }
             },
             [
diff --git a/workflows/sarek.nf b/workflows/sarek.nf
index 353a63d0c8..a741950588 100644
--- a/workflows/sarek.nf
+++ b/workflows/sarek.nf
@@ -239,6 +239,7 @@ workflow SAREK {
                 known_sites_indels,
                 known_sites_indels_tbi,
                 bbsplit_index,
+                params.save_output_as_bam,
             )
 
             // Gather preprocessing output

From bd3e9bc999e97eadcc1f0ebdd1252272aace289f Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Fri, 31 Jul 2026 11:36:48 +0200
Subject: [PATCH 23/27] Template update for nf-core/tools v4.0.3 (#2248)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Resolves the template sync up to nf-core/tools v4.0.3 (squashed into a
single commit for a clean diff).

### Key resolutions:
- Kept sarek's custom `main.nf`, `nextflow_schema.json`,
`tests/default.nf.test`, and `.nftignore`
- Accepted updated CI workflows, `.pre-commit-config.yaml`,
`nf-test.config`, fastqc/multiqc module updates (with conda-lock files),
and new `docs/CONTRIBUTING.md` location
- Fixed `UTILS_NFCORE_PIPELINE` call in
`utils_nfcore_sarek_pipeline/main.nf` — removed extra `null` argument
that no longer matches updated subworkflow signature

### Template changes included:
- Nextflow minimum bumped to 25.10.4
- `prek` replaces `pre-commit`
- Container configs auto-generated (conda-lock files)
- `CONTRIBUTING.md` moved from `.github/` to `docs/`
- Updated FastQC and MultiQC modules
- New `pr-comment.yml` workflow replaces `linting_comment.yml`

---------

Co-authored-by: Claude Sonnet 5 <noreply@anthropic.com>
---
 .github/actions/nf-test/action.yml            |   10 +-
 .github/workflows/branch.yml                  |   60 +-
 .github/workflows/linting.yml                 |   18 +
 .github/workflows/linting_comment.yml         |   28 -
 .github/workflows/nf-test-gpu.yml             |   10 +-
 .github/workflows/nf-test-sentieon.yml        |   13 +-
 .github/workflows/nf-test.yml                 |   72 +-
 .github/workflows/pr-comment.yml              |   82 +
 .github/workflows/release-announcements.yml   |    2 +-
 .../workflows/template-version-comment.yml    |   56 +-
 .gitignore                                    |    1 +
 .nf-core.yml                                  |    5 +-
 .pre-commit-config.yaml                       |   16 +-
 conf/base.config                              |    2 +-
 conf/modules/markduplicates.config            |    8 +-
 conf/modules/prepare_recalibration.config     |   12 +-
 conf/modules/recalibrate.config               |   12 +-
 main.nf                                       |    4 +-
 modules.json                                  |    2 +-
 .../linux_amd64-bd-c1f4a7982b743963_1.txt     | 1552 +++++++++++++++++
 .../linux_amd64-bd-db7c73dae76bc9e6_1.txt     |  126 ++
 .../linux_arm64-bd-40bf3b435e89dc22_1.txt     | 1502 ++++++++++++++++
 .../linux_arm64-bd-d167b8012595a136_1.txt     |  125 ++
 .../multiqc/tests/custom_prefix.config        |    5 +
 nextflow.config                               |    4 +-
 subworkflows/local/prepare_intervals/main.nf  |    2 +-
 .../local/utils_nfcore_sarek_pipeline/main.nf |    3 +-
 .../utils_nfcore_pipeline/tests/main.nf.test  |   29 +
 .../tests/main.nf.test.snap                   |   19 +
 .../nf-core/utils_nfschema_plugin/main.nf     |   12 +-
 .../nf-core/utils_nfschema_plugin/meta.yml    |   24 -
 tests/nextflow.config                         |    2 +-
 32 files changed, 3659 insertions(+), 159 deletions(-)
 delete mode 100644 .github/workflows/linting_comment.yml
 create mode 100644 .github/workflows/pr-comment.yml
 create mode 100644 modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c1f4a7982b743963_1.txt
 create mode 100644 modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-db7c73dae76bc9e6_1.txt
 create mode 100644 modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-40bf3b435e89dc22_1.txt
 create mode 100644 modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-d167b8012595a136_1.txt
 create mode 100644 modules/nf-core/multiqc/tests/custom_prefix.config
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
 create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap

diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
index 434f7b247e..15e293ad70 100644
--- a/.github/actions/nf-test/action.yml
+++ b/.github/actions/nf-test/action.yml
@@ -24,24 +24,24 @@ runs:
   using: "composite"
   steps:
     - name: Setup Nextflow
-      uses: nf-core/setup-nextflow@v2
+      uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
       with:
         version: "${{ env.NXF_VERSION }}"
 
     - name: Set up Python
-      uses: actions/setup-python@e797f83bcb11b83ae66e0230d6156d7c80228e7c # v6
+      uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
       with:
         python-version: "3.14"
 
     - name: Install nf-test
-      uses: nf-core/setup-nf-test@v1
+      uses: nf-core/setup-nf-test@4069fbbaabe94c08faba4ad261bfa88225ba133f # v2
       with:
         version: "${{ env.NFT_VER }}"
         install-pdiff: true
 
     - name: Setup apptainer
       if: contains(inputs.profile, 'singularity')
-      uses: eWaterCycle/setup-apptainer@main
+      uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0
 
     - name: Set up Singularity
       if: contains(inputs.profile, 'singularity')
@@ -52,7 +52,7 @@ runs:
 
     - name: Conda setup
       if: contains(inputs.profile, 'conda')
-      uses: conda-incubator/setup-miniconda@505e6394dae86d6a5c7fbb6e3fb8938e3e863830 # v3
+      uses: conda-incubator/setup-miniconda@8ee1f361103df19b6f8c8655fd3967a8ecb162d5 # v4
       with:
         auto-update-conda: true
         conda-solver: libmamba
diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml
index ce61ff29f5..2553896f1c 100644
--- a/.github/workflows/branch.yml
+++ b/.github/workflows/branch.yml
@@ -2,11 +2,13 @@ name: nf-core branch protection
 # This workflow is triggered on PRs to `main`/`master` branch on the repository
 # It fails when someone tries to make a PR against the nf-core `main`/`master` branch instead of `dev`
 on:
-  pull_request_target:
+  pull_request:
     branches:
       - main
       - master
 
+permissions: {}
+
 jobs:
   test:
     runs-on: ubuntu-latest
@@ -14,33 +16,47 @@ jobs:
       # PRs to the nf-core repo main/master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches
       - name: Check PRs
         if: github.repository == 'nf-core/sarek'
+        env:
+          HEAD_REPO: ${{ github.event.pull_request.head.repo.full_name }}
         run: |
-          { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/sarek ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
+          { [[ "$HEAD_REPO" == nf-core/sarek ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]]
 
-      # If the above check failed, post a comment on the PR explaining the failure
-      # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets
-      - name: Post PR comment
+      # If the above check failed, build a comment to be posted by the shared poster workflow
+      - name: Build PR comment
         if: failure()
-        uses: mshick/add-pr-comment@8e4927817251f1ff60c001f04568532b38e0b4a0 # v3
-        with:
-          message: |
-            ## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x:
+        env:
+          PR_NUMBER: ${{ github.event.pull_request.number }}
+          BASE_REF: ${{ github.event.pull_request.base.ref }}
+          HEAD_REPO: ${{ github.event.pull_request.head.repo.full_name }}
+          PR_USER: ${{ github.event.pull_request.user.login }}
+        run: |
+          mkdir -p pr-comment
+          echo "$PR_NUMBER" > pr-comment/pr_number.txt
+          echo "branch" > pr-comment/header.txt
+          cat > pr-comment/comment.md <<EOF
+          ## This PR is against the \`${BASE_REF}\` branch :x:
 
-            * Do not close this PR
-            * Click _Edit_ and change the `base` to `dev`
-            * This CI test will remain failed until you push a new commit
+          * Do not close this PR
+          * Click _Edit_ and change the \`base\` to \`dev\`
+          * This CI test will remain failed until you push a new commit
 
-            ---
+          ---
 
-            Hi @${{ github.event.pull_request.user.login }},
+          Hi @${PR_USER},
 
-            It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) ${{github.event.pull_request.base.ref}} branch.
-            The ${{github.event.pull_request.base.ref}} branch on nf-core repositories should always contain code from the latest release.
-            Because of this, PRs to ${{github.event.pull_request.base.ref}} are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch.
+          It looks like this pull-request is has been made against the [${HEAD_REPO}](https://github.com/${HEAD_REPO}) ${BASE_REF} branch.
+          The ${BASE_REF} branch on nf-core repositories should always contain code from the latest release.
+          Because of this, PRs to ${BASE_REF} are only allowed if they come from the [${HEAD_REPO}](https://github.com/${HEAD_REPO}) \`dev\` branch.
 
-            You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page.
-            Note that even after this, the test will continue to show as failing until you push a new commit.
+          You do not need to close this PR, you can change the target branch to \`dev\` by clicking the _"Edit"_ button at the top of this page.
+          Note that even after this, the test will continue to show as failing until you push a new commit.
 
-            Thanks again for your contribution!
-          repo-token: ${{ secrets.GITHUB_TOKEN }}
-          allow-repeats: false
+          Thanks again for your contribution!
+          EOF
+
+      - name: Upload PR comment artifact
+        if: failure()
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+        with:
+          name: pr-comment
+          path: pr-comment/
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index 8738ffc990..bc32219b2f 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -74,3 +74,21 @@ jobs:
             lint_log.txt
             lint_results.md
             PR_number.txt
+
+      # Build a comment for the shared pr-comment.yml poster to publish on the PR
+      - name: Prepare PR comment
+        if: ${{ always() }}
+        env:
+          PR_NUMBER: ${{ github.event.pull_request.number }}
+        run: |
+          mkdir -p pr-comment
+          echo "$PR_NUMBER" > pr-comment/pr_number.txt
+          echo "lint" > pr-comment/header.txt
+          [ -f lint_results.md ] && cp lint_results.md pr-comment/comment.md || true
+
+      - name: Upload PR comment artifact
+        if: ${{ always() }}
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+        with:
+          name: pr-comment
+          path: pr-comment/
diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml
deleted file mode 100644
index 5b0c24f751..0000000000
--- a/.github/workflows/linting_comment.yml
+++ /dev/null
@@ -1,28 +0,0 @@
-name: nf-core linting comment
-# This workflow is triggered after the linting action is complete
-# It posts an automated comment to the PR, even if the PR is coming from a fork
-
-on:
-  workflow_run:
-    workflows: ["nf-core linting"]
-
-jobs:
-  test:
-    runs-on: ubuntu-latest
-    steps:
-      - name: Download lint results
-        uses: dawidd6/action-download-artifact@b6e2e70617bc3265edd6dab6c906732b2f1ae151 # v21
-        with:
-          workflow: linting.yml
-          workflow_conclusion: completed
-
-      - name: Get PR number
-        id: pr_number
-        run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT
-
-      - name: Post PR comment
-        uses: marocchino/sticky-pull-request-comment@70d2764d1a7d5d9560b100cbea0077fc8f633987 # v3
-        with:
-          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
-          number: ${{ steps.pr_number.outputs.pr_number }}
-          path: linting-logs/lint_results.md
diff --git a/.github/workflows/nf-test-gpu.yml b/.github/workflows/nf-test-gpu.yml
index 07734a673b..059352ce7f 100644
--- a/.github/workflows/nf-test-gpu.yml
+++ b/.github/workflows/nf-test-gpu.yml
@@ -31,9 +31,7 @@ env:
 jobs:
   nf-test-gpu-changes:
     name: nf-test-gpu-changes
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test-gpu-changes
-      - runner=4cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -67,7 +65,7 @@ jobs:
     name: "GPU | ${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-gpu-changes.outputs.total_shards }}"
     needs: [nf-test-gpu-changes]
     if: ${{ needs.nf-test-gpu-changes.outputs.total_shards != '0' }}
-    runs-on: "runs-on=${{ github.run_id }}/family=g4dn.xlarge/image=ubuntu24-gpu-x64"
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down (no GPU available)
     strategy:
       fail-fast: false
       matrix:
@@ -132,9 +130,7 @@ jobs:
   confirm-pass:
     needs: [nf-test-gpu]
     if: always()
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-confirm-pass
-      - runner=2cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
diff --git a/.github/workflows/nf-test-sentieon.yml b/.github/workflows/nf-test-sentieon.yml
index cdf6f5530c..29aee33802 100644
--- a/.github/workflows/nf-test-sentieon.yml
+++ b/.github/workflows/nf-test-sentieon.yml
@@ -31,9 +31,7 @@ env:
 jobs:
   nf-test-sentieon-changes:
     name: nf-test-sentieon-changes
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test-sentieon-changes
-      - runner=4cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -67,10 +65,7 @@ jobs:
     name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-sentieon-changes.outputs.total_shards }}"
     needs: [nf-test-sentieon-changes]
     if: ${{ needs.nf-test-sentieon-changes.outputs.total_shards != '0' }}
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test-sentieon
-      - runner=4cpu-linux-x64
-      - volume=80gb
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     strategy:
       fail-fast: false
       matrix:
@@ -133,9 +128,7 @@ jobs:
   confirm-pass:
     needs: [nf-test-sentieon]
     if: always()
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-confirm-pass
-      - runner=2cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index 29987b5c7a..555cee8f5e 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -32,9 +32,7 @@ env:
 jobs:
   nf-test-changes:
     name: nf-test-changes
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test-changes
-      - runner=4cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -55,8 +53,8 @@ jobs:
         env:
           NFT_VER: ${{ env.NFT_VER }}
         with:
-          max_shards: 15
           tags: "cpu,cpu_conda"
+          max_shards: 15
           all_tests: ${{ github.event.inputs.update_snapshots || 'false' }}
 
       - name: debug
@@ -68,10 +66,7 @@ jobs:
     name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-changes.outputs.total_shards }}"
     needs: [nf-test-changes]
     if: ${{ needs.nf-test-changes.outputs.total_shards != '0' }}
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-nf-test
-      - runner=4cpu-linux-x64
-      - volume=80gb
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     strategy:
       fail-fast: false
       matrix:
@@ -126,12 +121,26 @@ jobs:
             fi
           fi
 
+      # continue-on-error keeps latest-everything from failing the job, so it never shows up in
+      # `needs.nf-test.result` downstream and CI stays green. Surface it via a PR comment instead;
+      # other NXF_VER failures already fail the job/CI directly, so no comment is needed for those.
+      - name: Prepare PR comment fragment
+        if: ${{ always() && steps.run_nf_test.outcome == 'failure' && matrix.NXF_VER == 'latest-everything' }}
+        run: |
+          mkdir -p pr-comment-fragment
+          echo "* ❌ \`${{ matrix.profile }}\` | \`${{ matrix.NXF_VER }}\` | Shard ${{ matrix.shard }}/${{ env.TOTAL_SHARDS }}" > pr-comment-fragment/fragment.md
+
+      - name: Upload PR comment fragment
+        if: ${{ always() && steps.run_nf_test.outcome == 'failure' && matrix.NXF_VER == 'latest-everything' }}
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+        with:
+          name: pr-comment-fragment-${{ strategy.job-index }}
+          path: pr-comment-fragment/
+
   confirm-pass:
     needs: [nf-test]
     if: always()
-    runs-on: # use self-hosted runners
-      - runs-on=${{ github.run_id }}-confirm-pass
-      - runner=2cpu-linux-x64
+    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
@@ -152,3 +161,44 @@ jobs:
           echo "DEBUG: toJSON(needs) = ${{ toJSON(needs) }}"
           echo "DEBUG: toJSON(needs.*.result) = ${{ toJSON(needs.*.result) }}"
           echo "::endgroup::"
+
+      - name: Download PR comment fragments
+        if: ${{ always() }}
+        uses: actions/download-artifact@3e5f45b2cfb9172054b4087a40e8e0b5a5461e7c # v8.0.1
+        continue-on-error: true
+        with:
+          pattern: pr-comment-fragment-*
+          path: pr-comment-fragments
+          merge-multiple: true
+
+      # Build a comment for the shared pr-comment.yml poster to publish on the PR.
+      # Based on the fragments above (not needs.*.result) so non-blocking failures are still reported.
+      - name: Prepare PR comment
+        if: ${{ always() }}
+        env:
+          PR_NUMBER: ${{ github.event.pull_request.number }}
+          RUN_URL: ${{ github.server_url }}/${{ github.repository }}/actions/runs/${{ github.run_id }}
+        run: |
+          mkdir -p pr-comment
+          echo "$PR_NUMBER" > pr-comment/pr_number.txt
+          echo "nf-test" > pr-comment/header.txt
+          if [ -d pr-comment-fragments ] && [ -n "$(ls -A pr-comment-fragments)" ]; then
+            {
+              echo "## ❌ nf-test failed with latest Nextflow version"
+              echo ""
+              echo "> [!NOTE]"
+              echo "> Tests with Nextflow's latest version failed but it will not cause a CI workflow failure."
+              echo "> Please check if the failure is expected with newer (edge-)releases of Nextflow or if it needs fixing."
+              echo ""
+              cat pr-comment-fragments/*.md
+              echo ""
+              echo "See the [full run](${RUN_URL}) for details."
+            } > pr-comment/comment.md
+          fi
+
+      - name: Upload PR comment artifact
+        if: ${{ always() }}
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
+        with:
+          name: pr-comment
+          path: pr-comment/
diff --git a/.github/workflows/pr-comment.yml b/.github/workflows/pr-comment.yml
new file mode 100644
index 0000000000..ab7b59ddf1
--- /dev/null
+++ b/.github/workflows/pr-comment.yml
@@ -0,0 +1,82 @@
+name: Post PR comment
+# Shared, privileged comment poster.
+#
+# This is the single workflow that runs with a write token. It is triggered
+# after any of the listed "producer" workflows complete on a pull request.
+# Each producer runs untrusted PR code (if any) with a read-only token and
+# uploads a `pr-comment` artifact describing the comment to post; this workflow
+# only ever reads that plain-text artifact, so no PR code is executed here.
+#
+# Artifact contract (uploaded by producers under the name `pr-comment`):
+#   pr_number.txt  - the pull request number
+#   header.txt     - sticky-comment identifier (keeps comment types separate)
+#   comment.md     - the Markdown body (omit the file to post nothing)
+
+on:
+  workflow_run:
+    workflows:
+      - "nf-core linting"
+      - "nf-core template version comment"
+      - "nf-core branch protection"
+      - "Run nf-test"
+
+permissions:
+  actions: read
+  contents: read
+  pull-requests: write
+
+jobs:
+  post-comment:
+    runs-on: ubuntu-latest
+    if: github.event.workflow_run.event == 'pull_request'
+    steps:
+      - name: Download PR comment artifact
+        uses: dawidd6/action-download-artifact@b6e2e70617bc3265edd6dab6c906732b2f1ae151 # v21
+        with:
+          run_id: ${{ github.event.workflow_run.id }}
+          name: pr-comment
+          path: pr-comment
+          if_no_artifact_found: ignore
+
+      - name: Read comment metadata
+        id: meta
+        run: |
+          echo "::group::Downloaded pr-comment contents"
+          ls -la pr-comment 2>/dev/null || echo "No pr-comment/ directory was downloaded."
+          echo "::endgroup::"
+
+          if [ ! -d pr-comment ]; then
+            echo "No pr-comment artifact found; nothing to post."
+            exit 0
+          fi
+
+          if [ ! -f pr-comment/comment.md ]; then
+            echo "Artifact present but no comment.md; nothing to post."
+            exit 0
+          fi
+
+          pr_number=$(cat pr-comment/pr_number.txt)
+          header=$(cat pr-comment/header.txt)
+          echo "Found comment.md (header='$header', pr_number='$pr_number')."
+
+          # Guard against anything unexpected ending up in the PR number.
+          case "$pr_number" in
+            ''|*[!0-9]*)
+              echo "Invalid PR number: '$pr_number'"
+              exit 1
+              ;;
+          esac
+
+          echo "pr_number=$pr_number" >> "$GITHUB_OUTPUT"
+          echo "header=$header" >> "$GITHUB_OUTPUT"
+          echo "post=true" >> "$GITHUB_OUTPUT"
+          echo "Will post comment to PR #${pr_number}."
+
+      - name: Post PR comment
+        if: steps.meta.outputs.post == 'true'
+        uses: marocchino/sticky-pull-request-comment@5770ad5eb8f42dd2c4f34da00c94c5381e49af88 # v3.0.5
+        with:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
+          number: ${{ steps.meta.outputs.pr_number }}
+          header: ${{ steps.meta.outputs.header }}
+          path: pr-comment/comment.md
diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml
index 78d5dbe056..4974f44f27 100644
--- a/.github/workflows/release-announcements.yml
+++ b/.github/workflows/release-announcements.yml
@@ -18,7 +18,7 @@ jobs:
         id: get_description
         run: |
           echo "description=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .description')" >> $GITHUB_OUTPUT
-      - uses: rzr/fediverse-action@563159eb8d45f70ab6aaba36ed55cd037e51f441 # master
+      - uses: rzr/fediverse-action@66c2cbb5b1997666b0e28d597631b6a4f09a2719 # v0.0.6
         with:
           access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }}
           host: "mstdn.science" # custom host if not "mastodon.social" (default)
diff --git a/.github/workflows/template-version-comment.yml b/.github/workflows/template-version-comment.yml
index ea30827ec8..ee102f71f1 100644
--- a/.github/workflows/template-version-comment.yml
+++ b/.github/workflows/template-version-comment.yml
@@ -2,10 +2,13 @@ name: nf-core template version comment
 # This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version.
 # It posts a comment to the PR, even if it comes from a fork.
 
-on: pull_request_target
+on:
+  pull_request:
+
+permissions: {}
 
 jobs:
-  template_version:
+  check_template_version:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
@@ -22,25 +25,36 @@ jobs:
       - name: Install nf-core
         run: |
           python -m pip install --upgrade pip
-          pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
+          pip install nf-core
+
+      - name: Build PR comment if template is outdated
+        # The fork-controlled version is passed via the environment and only ever
+        # used as quoted shell data (never interpolated into a command), so it
+        # cannot be used for script injection.
+        env:
+          PR_VERSION: ${{ steps.read_yml.outputs['nf_core_version'] }}
+          PR_NUMBER: ${{ github.event.pull_request.number }}
+        run: |
+          mkdir -p pr-comment
+          echo "$PR_NUMBER" > pr-comment/pr_number.txt
+          echo "template-version" > pr-comment/header.txt
+
+          latest_version=$(nf-core --version | grep -oE '[0-9]+\.[0-9]+\.[0-9]+' | head -n1)
 
-      - name: Check nf-core outdated
-        id: nf_core_outdated
-        run: echo "OUTPUT=$(pip list --outdated | grep nf-core)" >> ${GITHUB_ENV}
+          if [ -n "$PR_VERSION" ] && [ -n "$latest_version" ] && [ "$PR_VERSION" != "$latest_version" ]; then
+            cat > pr-comment/comment.md <<EOF
+          > [!WARNING]
+          > Newer version of the nf-core template is available.
+          >
+          > Your pipeline is using an old version of the nf-core template: ${PR_VERSION}.
+          > Please update your pipeline to the latest version.
+          >
+          > For more documentation on how to update your pipeline, please see the [Synchronisation documentation](https://nf-co.re/docs/developing/template-syncs/overview).
+          EOF
+          fi
 
-      - name: Post nf-core template version comment
-        uses: mshick/add-pr-comment@8e4927817251f1ff60c001f04568532b38e0b4a0 # v3
-        if: |
-          contains(env.OUTPUT, 'nf-core')
+      - name: Upload PR comment artifact
+        uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
         with:
-          repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }}
-          allow-repeats: false
-          message: |
-            > [!WARNING]
-            > Newer version of the nf-core template is available.
-            >
-            > Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}.
-            > Please update your pipeline to the latest version.
-            >
-            > For more documentation on how to update your pipeline, please see the [Synchronisation documentation](https://nf-co.re/docs/developing/template-syncs/overview).
-          #
+          name: pr-comment
+          path: pr-comment/
diff --git a/.gitignore b/.gitignore
index e3aaef5619..4c857caa24 100644
--- a/.gitignore
+++ b/.gitignore
@@ -16,3 +16,4 @@ test.xml
 .vscode/mcp.json
 modules/nf-core/**/tests/
 subworkflows/nf-core/**/tests/
+.lineage/
diff --git a/.nf-core.yml b/.nf-core.yml
index cd09c125f6..bc2565cfb0 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -13,12 +13,11 @@ lint:
   nf_test_content: false
   schema_params: false
   template_strings: false
-nf_core_version: 4.0.2
+nf_core_version: 4.0.3
 repository_type: pipeline
 template:
   author: Maxime Garcia, Szilveszter Juhos, Friederike Hanssen
-  description: An open-source analysis pipeline to detect germline or somatic variants
-    from whole genome or targeted sequencing
+  description: An open-source analysis pipeline to detect germline or somatic variants from whole genome or targeted sequencing
   force: false
   is_nfcore: true
   name: sarek
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index d06777a8f7..f51e1a28da 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -4,7 +4,7 @@ repos:
     hooks:
       - id: prettier
         additional_dependencies:
-          - prettier@3.6.2
+          - prettier@3.8.3
   - repo: https://github.com/pre-commit/pre-commit-hooks
     rev: v6.0.0
     hooks:
@@ -13,15 +13,21 @@ repos:
         exclude: |
           (?x)^(
               .*ro-crate-metadata.json$|
-              modules/nf-core/.*|
-              subworkflows/nf-core/.*|
+              modules/(?!local/).*|
+              subworkflows/(?!local/).*|
               .*\.snap$
           )$
       - id: end-of-file-fixer
         exclude: |
           (?x)^(
               .*ro-crate-metadata.json$|
-              modules/nf-core/.*|
-              subworkflows/nf-core/.*|
+              modules/(?!local/).*|
+              subworkflows/(?!local/).*|
               .*\.snap$
           )$
+  - repo: https://github.com/seqeralabs/nf-lint-pre-commit
+    rev: v0.3.0
+    hooks:
+      - id: nextflow-lint
+        files: '\.nf$|nextflow\.config$'
+        args: ["-output", "json"]
diff --git a/conf/base.config b/conf/base.config
index 63d7104837..ec3d42b963 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -15,7 +15,7 @@ process {
     memory = { 6.GB   * task.attempt }
     time   = { 8.h    * task.attempt }
 
-    errorStrategy = { task.exitStatus in ((130..145) + 104 + 175) ? 'retry' : 'finish' }
+    errorStrategy = { task.exitStatus in ((130..145) + 104 + (175..177)) ? 'retry' : 'finish' }
     maxRetries    = 1
     maxErrors     = '-1'
 
diff --git a/conf/modules/markduplicates.config b/conf/modules/markduplicates.config
index 9a0b81245b..612d9d28d2 100644
--- a/conf/modules/markduplicates.config
+++ b/conf/modules/markduplicates.config
@@ -91,7 +91,13 @@ process {
     }
 
     withName: 'GATK4SPARK_MARKDUPLICATES' {
-        containerOptions = ''
+        // Spark's native UnixLoginModule fails to resolve a username for the container's UID
+        // (LoginException: invalid null input: name), because the container's own /etc/passwd
+        // has no entry for the host UID that docker.runOptions maps it to. Bind-mounting the
+        // host's /etc/passwd/group (which do have that entry) fixes the native lookup.
+        containerOptions = { workflow.containerEngine in ['singularity', 'apptainer']
+            ? '--bind /etc/passwd:/etc/passwd:ro,/etc/group:/etc/group:ro'
+            : '-v /etc/passwd:/etc/passwd:ro -v /etc/group:/etc/group:ro' }
         ext.args   = { '--remove-sequencing-duplicates false -VS LENIENT' }
         ext.prefix = { params.save_output_as_bam ? "${meta.id}.md.bam" : "${meta.id}.md.cram" }
         publishDir = [
diff --git a/conf/modules/prepare_recalibration.config b/conf/modules/prepare_recalibration.config
index 98bcd9408d..4fd8e0dc55 100644
--- a/conf/modules/prepare_recalibration.config
+++ b/conf/modules/prepare_recalibration.config
@@ -16,7 +16,17 @@
 process {
 
     withName: 'GATK4_BASERECALIBRATOR|GATK4SPARK_BASERECALIBRATOR' {
-        containerOptions = params.use_gatk_spark ? '' : null
+        // Spark's native UnixLoginModule fails to resolve a username for the container's UID
+        // (LoginException: invalid null input: name), because the container's own /etc/passwd
+        // has no entry for the host UID that docker.runOptions maps it to. Bind-mounting the
+        // host's /etc/passwd/group (which do have that entry) fixes the native lookup.
+        containerOptions = {
+            params.use_gatk_spark
+                ? (workflow.containerEngine in ['singularity', 'apptainer']
+                    ? '--bind /etc/passwd:/etc/passwd:ro,/etc/group:/etc/group:ro'
+                    : '-v /etc/passwd:/etc/passwd:ro -v /etc/group:/etc/group:ro')
+                : null
+        }
         ext.prefix       = { meta.num_intervals <= 1 ? "${meta.id}.recal" : "${meta.id}_${intervals.simpleName}.recal" }
         publishDir       = [
             mode: params.publish_dir_mode,
diff --git a/conf/modules/recalibrate.config b/conf/modules/recalibrate.config
index dc10e1f5b1..28943869b2 100644
--- a/conf/modules/recalibrate.config
+++ b/conf/modules/recalibrate.config
@@ -16,7 +16,17 @@
 process {
 
     withName: 'GATK4_APPLYBQSR|GATK4SPARK_APPLYBQSR' {
-        containerOptions = params.use_gatk_spark ? '' : null
+        // Spark's native UnixLoginModule fails to resolve a username for the container's UID
+        // (LoginException: invalid null input: name), because the container's own /etc/passwd
+        // has no entry for the host UID that docker.runOptions maps it to. Bind-mounting the
+        // host's /etc/passwd/group (which do have that entry) fixes the native lookup.
+        containerOptions = {
+            params.use_gatk_spark
+                ? (workflow.containerEngine in ['singularity', 'apptainer']
+                    ? '--bind /etc/passwd:/etc/passwd:ro,/etc/group:/etc/group:ro'
+                    : '-v /etc/passwd:/etc/passwd:ro -v /etc/group:/etc/group:ro')
+                : null
+        }
         ext.prefix       = {
             meta.num_intervals <= 1 ? "${meta.id}.recal" : "${meta.id}_${intervals.baseName}.recal"
         }
diff --git a/main.nf b/main.nf
index 1ea13f281a..74c63b91ba 100755
--- a/main.nf
+++ b/main.nf
@@ -359,7 +359,7 @@ workflow {
         params.input,
         params.help,
         params.help_full,
-        params.show_hidden,
+        params.show_hidden
     )
 
     //
@@ -376,7 +376,7 @@ workflow {
         params.plaintext_email,
         params.outdir,
         params.monochrome_logs,
-        NFCORE_SAREK.out.multiqc_report,
+        NFCORE_SAREK.out.multiqc_report
     )
 
     publish:
diff --git a/modules.json b/modules.json
index 2c414088c3..bc7c99307b 100644
--- a/modules.json
+++ b/modules.json
@@ -637,7 +637,7 @@
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "a7b27fd25bfa8dcc07d299e88bd790585901a436",
+                        "git_sha": "fdc08b8b1ae74f56686ce21f7ea11ad11990ce57",
                         "installed_by": ["subworkflows"]
                     },
                     "vcf_annotate_snpeff": {
diff --git a/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c1f4a7982b743963_1.txt b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c1f4a7982b743963_1.txt
new file mode 100644
index 0000000000..761903040d
--- /dev/null
+++ b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c1f4a7982b743963_1.txt
@@ -0,0 +1,1552 @@
+
+version: 6
+environments:
+default:
+channels:
+- url: https://conda.anaconda.org/conda-forge/
+- url: https://conda.anaconda.org/bioconda/
+- url: https://conda.anaconda.org/bioconda/
+options:
+pypi-prerelease-mode: if-necessary-or-explicit
+packages:
+linux-64:
+- conda: https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-20_gnu.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/_python_abi3_support-1.0-hd8ed1ab_2.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/annotated-types-0.7.0-pyhd8ed1ab_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/attrs-26.1.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/backports.zstd-1.3.0-py314h680f03e_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-python-1.2.0-py314h3de4e8d_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.2.25-hbd8a1cb_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/certifi-2026.2.25-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.6-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/click-8.3.1-pyh8f84b5b_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/coloredlogs-15.0.1-pyhd8ed1ab_4.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/colormath-3.0.0-pyhd8ed1ab_4.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/cpython-3.14.3-py314hd8ed1ab_101.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/expat-2.7.4-hecca717_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-dejavu-sans-mono-2.37-hab24e00_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-inconsolata-3.000-h77eed37_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-source-code-pro-2.038-h77eed37_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/noarch/font-ttf-ubuntu-0.83-h77eed37_3.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/fontconfig-2.17.1-h27c8c51_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/fonts-conda-forge-1-hc364b38_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/h2-4.3.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/hpack-4.1.0-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/humanfriendly-10.0-pyh707e725_8.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/humanize-4.15.0-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/hyperframe-6.1.0-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/icu-78.3-h33c6efd_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/idna-3.11-pyhd8ed1ab_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/importlib-metadata-8.8.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/jinja2-3.1.6-pyhcf101f3_1.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-4.26.0-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/noarch/jsonschema-specifications-2025.9.1-pyhcf101f3_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/kaleido-core-0.2.1-h3644ca4_0.tar.bz2
+- conda: https://conda.anaconda.org/conda-forge/linux-64/lcms2-2.18-h0c24ade_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/ld_impl_linux-64-2.45.1-default_hbd61a6d_102.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/lerc-4.1.0-hdb68285_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libblas-3.11.0-5_h4a7cf45_openblas.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libcblas-3.11.0-5_h0358290_openblas.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libdeflate-1.25-h17f619e_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libexpat-2.7.4-hecca717_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.5.2-h3435931_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype-2.14.3-ha770c72_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libfreetype6-2.14.3-h73754d4_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-15.2.0-he0feb66_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgcc-ng-15.2.0-h69a702a_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgfortran-15.2.0-h69a702a_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgfortran5-15.2.0-h68bc16d_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libgomp-15.2.0-he0feb66_18.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libjpeg-turbo-3.1.2-hb03c661_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/liblapack-3.11.0-5_h47877c9_openblas.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/liblzma-5.8.2-hb03c661_0.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libmpdec-4.0.0-hb03c661_1.conda
+- conda: https://conda.anaconda.org/conda-forge/linux-64/libopenblas-0.3.30-pthreads_h94d23a6_4.conda
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+- icu >=78.2,<79.0a0
+- libgcc >=14
+- libsqlite 3.52.0 hf4e2dac_0
+- libzlib >=1.3.1,<2.0a0
+- ncurses >=6.5,<7.0a0
+- readline >=8.3,<9.0a0
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+- libstdcxx >=14
+- python >=3.14,<3.15.0a0
+- python_abi 3.14.* *_cp314
+- regex >=2022.1.18
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+- conda: https://conda.anaconda.org/conda-forge/noarch/tqdm-4.67.3-pyh8f84b5b_0.conda
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diff --git a/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-db7c73dae76bc9e6_1.txt b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-db7c73dae76bc9e6_1.txt
new file mode 100644
index 0000000000..a55a4d49d2
--- /dev/null
+++ b/modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-db7c73dae76bc9e6_1.txt
@@ -0,0 +1,126 @@
+
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-64
+@EXPLICIT
+https://conda.anaconda.org/conda-forge/linux-64/libgomp-15.2.0-he0feb66_18.conda#239c5e9546c38a1e884d69effcf4c882
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+https://conda.anaconda.org/conda-forge/linux-64/libgcc-15.2.0-he0feb66_18.conda#0aa00f03f9e39fb9876085dee11a85d4
+https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda#d2ffd7602c02f2b316fd921d39876885
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+timestamp: 1764460141901
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+md5: f731af71c723065d91b4c01bb822641b
+depends:
+- libgcc >=14
+- libstdcxx >=14
+license: Zlib
+license_family: Other
+size: 121046
+timestamp: 1770167944449
+- conda: https://conda.anaconda.org/conda-forge/linux-aarch64/zstd-1.5.7-h85ac4a6_6.conda
+sha256: 569990cf12e46f9df540275146da567d9c618c1e9c7a0bc9d9cfefadaed20b75
+md5: c3655f82dcea2aa179b291e7099c1fcc
+depends:
+- libzlib >=1.3.1,<2.0a0
+license: BSD-3-Clause
+license_family: BSD
+size: 614429
+timestamp: 1764777145593
diff --git a/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-d167b8012595a136_1.txt b/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-d167b8012595a136_1.txt
new file mode 100644
index 0000000000..f787dbe1e8
--- /dev/null
+++ b/modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-d167b8012595a136_1.txt
@@ -0,0 +1,125 @@
+
+# This file may be used to create an environment using:
+# $ conda create --name <env> --file <this file>
+# platform: linux-aarch64
+@EXPLICIT
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+https://conda.anaconda.org/conda-forge/linux-aarch64/bzip2-1.0.8-h4777abc_9.conda#840d8fc0d7b3209be93080bc20e07f2d
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+https://conda.anaconda.org/conda-forge/noarch/tqdm-4.67.3-pyh8f84b5b_0.conda#e5ce43272193b38c2e9037446c1d9206
+https://conda.anaconda.org/conda-forge/noarch/typeguard-4.5.1-pyhd8ed1ab_0.conda#260af1b0a94f719de76b4e14094e9a3b
+https://conda.anaconda.org/bioconda/noarch/multiqc-1.34-pyhdfd78af_0.conda#a7111ab9a6a6146b40cbce16655ac873
+https://conda.anaconda.org/conda-forge/noarch/pip-26.0.1-pyh145f28c_0.conda#09a970fbf75e8ed1aa633827ded6aa4f
+https://conda.anaconda.org/conda-forge/linux-aarch64/procps-ng-4.0.6-h1779866_0.conda#ab7288cc39545556d1bc5e71ab2df9a9
diff --git a/modules/nf-core/multiqc/tests/custom_prefix.config b/modules/nf-core/multiqc/tests/custom_prefix.config
new file mode 100644
index 0000000000..b30b1358b0
--- /dev/null
+++ b/modules/nf-core/multiqc/tests/custom_prefix.config
@@ -0,0 +1,5 @@
+process {
+    withName: 'MULTIQC' {
+        ext.prefix = "custom_prefix"
+    }
+}
diff --git a/nextflow.config b/nextflow.config
index 58794527b4..62a3287a31 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -158,7 +158,6 @@ params {
     email_on_fail                = null
     plaintext_email              = false
     monochrome_logs              = false
-
     help                         = false
     help_full                    = false
     show_hidden                  = false
@@ -180,7 +179,7 @@ params {
     validate_params            = true
 }
 
-// workflow output
+// Backwards compatibility for publishDir syntax
 outputDir = params.outdir
 workflow.output.mode = params.publish_dir_mode
 
@@ -782,7 +781,6 @@ validation {
     defaultIgnoreParams = ["genomes", "freebayes_filter", "vep_cache_version", "igenomes_base", "snpeff_cache", "vep_cache"]
     monochromeLogs = params.monochrome_logs
 }
-
 // Load modules.config for DSL2 module specific options
 includeConfig 'conf/modules/modules.config'
 
diff --git a/subworkflows/local/prepare_intervals/main.nf b/subworkflows/local/prepare_intervals/main.nf
index 52553eb5c0..295fb77a28 100644
--- a/subworkflows/local/prepare_intervals/main.nf
+++ b/subworkflows/local/prepare_intervals/main.nf
@@ -32,7 +32,7 @@ workflow PREPARE_INTERVALS {
         file("${outdir}/no_intervals.bed.gz.tbi").text = "no_intervals\n"
 
         intervals_bed        = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ bed -> [ bed, 0 ] }
-        intervals_bed_gz_tbi = channel.fromPath(file("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ files -> [ files, 0 ] }
+        intervals_bed_gz_tbi = channel.fromPath(files("${outdir}/no_intervals.bed.{gz,gz.tbi}")).collect().map{ files -> [ files, 0 ] }
         intervals_combined   = channel.fromPath(file("${outdir}/no_intervals.bed")).map{ bed -> [ [ id:bed.simpleName ], bed ] }
     } else if (step != 'annotate' && step != 'controlfreec') {
         // If no interval/target file is provided, then generated intervals from FASTA file
diff --git a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
index cd2d52bdcc..71d4f42c33 100644
--- a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
@@ -80,8 +80,7 @@ workflow PIPELINE_INITIALISATION {
         show_hidden,
         before_text,
         after_text,
-        command,
-        null,
+        command
     )
 
     // Check config provided to the pipeline
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
new file mode 100644
index 0000000000..8940d32d1e
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
@@ -0,0 +1,29 @@
+nextflow_workflow {
+
+    name "Test Workflow UTILS_NFCORE_PIPELINE"
+    script "../main.nf"
+    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
+    workflow "UTILS_NFCORE_PIPELINE"
+    tag "subworkflows"
+    tag "subworkflows_nfcore"
+    tag "utils_nfcore_pipeline"
+    tag "subworkflows/utils_nfcore_pipeline"
+
+    test("Should run without failures") {
+
+        when {
+            workflow {
+                """
+                input[0] = []
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert workflow.success },
+                { assert snapshot(workflow.out).match() }
+            )
+        }
+    }
+}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap
new file mode 100644
index 0000000000..859d1030fb
--- /dev/null
+++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap
@@ -0,0 +1,19 @@
+{
+    "Should run without failures": {
+        "content": [
+            {
+                "0": [
+                    true
+                ],
+                "valid_config": [
+                    true
+                ]
+            }
+        ],
+        "meta": {
+            "nf-test": "0.8.4",
+            "nextflow": "23.10.1"
+        },
+        "timestamp": "2024-02-28T12:03:25.726491"
+    }
+}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
index 9ff0681f15..1df8b76fba 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/main.nf
+++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
@@ -22,7 +22,6 @@ workflow UTILS_NFSCHEMA_PLUGIN {
     before_text         // string:   text to show before the help message and parameters summary
     after_text          // string:   text to show after the help message and parameters summary
     command             // string:   an example command of the pipeline
-    cli_typecast        // boolean:  whether to perform typecasting of CLI parameters. Set this to `null` to use the default behaviour
 
     main:
 
@@ -35,11 +34,11 @@ workflow UTILS_NFSCHEMA_PLUGIN {
             fullHelp: help_full,
         ]
         if(parameters_schema) {
-            help_options << [parameters_schema: parameters_schema]
+            help_options << [parametersSchema: parameters_schema]
         }
         log.info paramsHelp(
             help_options,
-            (help instanceof String && help != "true") ? help : "",
+            (params.help instanceof String && params.help != "true") ? params.help : "",
         )
         exit 0
     }
@@ -51,7 +50,7 @@ workflow UTILS_NFSCHEMA_PLUGIN {
 
     summary_options = [:]
     if(parameters_schema) {
-        summary_options << [parameters_schema: parameters_schema]
+        summary_options << [parametersSchema: parameters_schema]
     }
     log.info before_text
     log.info paramsSummaryLog(summary_options, input_workflow)
@@ -64,10 +63,7 @@ workflow UTILS_NFSCHEMA_PLUGIN {
     if(validate_params) {
         validateOptions = [:]
         if(parameters_schema) {
-            validateOptions << [parameters_schema: parameters_schema]
-        }
-        if(cli_typecast != null) {
-            validateOptions << [cast_cli_params: cli_typecast]
+            validateOptions << [parametersSchema: parameters_schema]
         }
         validateParameters(validateOptions)
     }
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
index 1d8c75a952..f7d9f02885 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
+++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
@@ -25,30 +25,6 @@ input:
         option. When this input is empty it will automatically use the configured schema or
         "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way
         for meta pipelines.
-  - help:
-      type: boolean, string
-      description: |
-        Show the help message and exit. When a parameter name is given, show the help message for that parameter instead of the general help message.
-  - help_full:
-      type: boolean
-      description: Show the full help message and exit.
-  - show_hidden:
-      type: boolean
-      description: Show hidden parameters in the help message.
-  - before_text:
-      type: string
-      description: Text to show before the parameters summary and help message.
-  - after_text:
-      type: string
-      description: Text to show after the parameters summary and help message.
-  - command:
-      type: string
-      description: An example command to run the pipeline, to show in the help message and the summary.
-  - cli_typecast:
-      type: boolean
-      description: |
-        Whether to apply typecasting to the parameters given via the CLI before validation.
-        Set this to `null` to use the default behavior.
 output:
   - dummy_emit:
       type: boolean
diff --git a/tests/nextflow.config b/tests/nextflow.config
index 54a47091b9..d62a3fa752 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -6,7 +6,7 @@
 
 params {
     modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
-    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/sarek/'
 }
 
 aws.client.anonymous = true // fixes S3 access issues on self-hosted runners

From c3cbfab868258f8c8ad686a1453ca1703286915c Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Tue, 4 Aug 2026 12:48:03 +0200
Subject: [PATCH 24/27] Important! Template update for nf-core/tools v4.1.0
 (#2255)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

Template update for nf-core/tools v4.1.0. Closes #2253.

Work on top of the sync:

- `conf/modules/*.config`: collapsed list-form `publishDir` into single
maps routing through `saveAs`. Required because 4.1.0 lints via
`nextflow config -o json`, which fails on a closure nested inside a
collection. Published layouts unchanged.
- `utils_nfschema_plugin`: renamed `parametersSchema:` →
`parameters_schema:` to match the recorded git_sha (fixes
`check_local_copy`). No runtime effect — sarek passes `null`.
- `tests/nextflow.config`, `tests/lib/UTILS.groovy`, `AGENTS.md`: small
missed/stale fixes.

## Checklist

- [x] This comment contains a description of changes (with reason)
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [x] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/blob/master/docs/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [x] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [x] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).

Generated with Claude Code

---------

Co-authored-by: Claude Opus 5 (1M context) <noreply@anthropic.com>
---
 .github/actions/nf-test/action.yml            |  6 +-
 .github/workflows/awsfulltest.yml             |  8 +-
 .github/workflows/clean-up.yml                |  2 +-
 .github/workflows/download_pipeline.yml       |  6 +-
 .github/workflows/fix_linting.yml             |  6 +-
 .github/workflows/linting.yml                 | 16 ++--
 .github/workflows/nf-test.yml                 |  4 +-
 .../workflows/template-version-comment.yml    |  2 +-
 .hooks/block_pipeline_outdir.sh               | 44 ++++++++++
 .nf-core.yml                                  |  2 +-
 .pre-commit-config.yaml                       | 19 +++-
 AGENTS.md                                     |  2 +-
 CHANGELOG.md                                  |  1 +
 README.md                                     |  8 +-
 conf/base.config                              |  3 +
 conf/containers_conda_lock_files_amd64.config |  4 +-
 conf/containers_conda_lock_files_arm64.config |  4 +-
 conf/modules/annotate.config                  | 33 +++----
 conf/modules/contamination.config             | 24 ++---
 conf/modules/markduplicates.config            | 88 ++++++++++---------
 conf/modules/modules.config                   |  8 +-
 conf/modules/recalibrate.config               | 35 ++++----
 conf/modules/sentieon_dedup.config            | 35 ++++----
 conf/modules/trimming.config                  | 25 +++---
 conf/modules/umi.config                       |  7 +-
 docs/output.md                                |  2 +-
 docs/usage.md                                 | 10 +--
 modules.json                                  |  4 +-
 .../multiqc/tests/custom_prefix.config        |  5 --
 ro-crate-metadata.json                        |  2 +-
 .../local/utils_nfcore_sarek_pipeline/main.nf |  5 +-
 .../utils_nfcore_pipeline/tests/main.nf.test  | 29 ------
 .../tests/main.nf.test.snap                   | 19 ----
 .../nf-core/utils_nfschema_plugin/main.nf     | 12 ++-
 .../nf-core/utils_nfschema_plugin/meta.yml    | 24 +++++
 tests/lib/UTILS.groovy                        |  2 +-
 tests/nextflow.config                         |  5 +-
 37 files changed, 281 insertions(+), 230 deletions(-)
 create mode 100755 .hooks/block_pipeline_outdir.sh
 delete mode 100644 modules/nf-core/multiqc/tests/custom_prefix.config
 delete mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
 delete mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap

diff --git a/.github/actions/nf-test/action.yml b/.github/actions/nf-test/action.yml
index 15e293ad70..9e19ba91aa 100644
--- a/.github/actions/nf-test/action.yml
+++ b/.github/actions/nf-test/action.yml
@@ -24,12 +24,12 @@ runs:
   using: "composite"
   steps:
     - name: Setup Nextflow
-      uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
+      uses: nf-core/setup-nextflow@893c28b667aedeba26e37f296d260ccc5bc4d914 # v3
       with:
         version: "${{ env.NXF_VERSION }}"
 
     - name: Set up Python
-      uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
+      uses: actions/setup-python@5fda3b95a4ea91299a34e894583c3862153e4b97 # v7
       with:
         python-version: "3.14"
 
@@ -41,7 +41,7 @@ runs:
 
     - name: Setup apptainer
       if: contains(inputs.profile, 'singularity')
-      uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0
+      uses: eWaterCycle/setup-apptainer@3f706d898c9db585b1d741b4692e66755f3a1b40 # v2.0.0
 
     - name: Set up Singularity
       if: contains(inputs.profile, 'singularity')
diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml
index fb4ff59bc6..a576004af5 100644
--- a/.github/workflows/awsfulltest.yml
+++ b/.github/workflows/awsfulltest.yml
@@ -86,7 +86,7 @@ jobs:
 
       - name: Launch workflow via Seqera Platform
         id: tower-launch
-        uses: seqeralabs/action-tower-launch@v2
+        uses: seqeralabs/action-tower-launch@51565b514bff1827cf34620de25d0055759f1fc9 # v2
         with:
           run_name: sarek_${{ matrix.profile }}
           workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
@@ -129,9 +129,9 @@ jobs:
             --repo ${{ github.repository }} \
             --body "Cloud test **${{ matrix.profile }}** launched on [Seqera Platform](${{ steps.tower-launch.outputs.workflowUrl }})"
 
-      - uses: actions/upload-artifact@330a01c490aca151604b8cf639adc76d48f6c5d4 # v5
+      - uses: actions/upload-artifact@043fb46d1a93c77aae656e7c1c64a875d1fc6a0a # v7
         with:
           name: Seqera Platform debug log file - ${{ matrix.profile }}
           path: |
-            seqera_platform_action_*.log
-            seqera_platform_action_*.json
+            tower_action_*.log
+            tower_action_*.json
diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml
index 172de6f373..b694012360 100644
--- a/.github/workflows/clean-up.yml
+++ b/.github/workflows/clean-up.yml
@@ -10,7 +10,7 @@ jobs:
       issues: write
       pull-requests: write
     steps:
-      - uses: actions/stale@b5d41d4e1d5dceea10e7104786b73624c18a190f # v10
+      - uses: actions/stale@4391f3da665fdf50b6810c1a66712fb9ba21aa93 # v11
         with:
           stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days."
           stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful."
diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml
index a7bf4fc21f..ca2eb82240 100644
--- a/.github/workflows/download_pipeline.yml
+++ b/.github/workflows/download_pipeline.yml
@@ -39,15 +39,15 @@ jobs:
     needs: configure
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+        uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
+        uses: nf-core/setup-nextflow@893c28b667aedeba26e37f296d260ccc5bc4d914 # v3
 
       - name: Disk space cleanup
         uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1
 
-      - uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
+      - uses: actions/setup-python@5fda3b95a4ea91299a34e894583c3862153e4b97 # v7
         with:
           python-version: "3.14"
           architecture: "x64"
diff --git a/.github/workflows/fix_linting.yml b/.github/workflows/fix_linting.yml
index ea83774c5d..fd9c335a5b 100644
--- a/.github/workflows/fix_linting.yml
+++ b/.github/workflows/fix_linting.yml
@@ -13,7 +13,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       # Use the @nf-core-bot token to check out so we can push later
-      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+      - uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
         with:
           token: ${{ secrets.nf_core_bot_auth_token }}
 
@@ -32,12 +32,12 @@ jobs:
           GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
+        uses: nf-core/setup-nextflow@893c28b667aedeba26e37f296d260ccc5bc4d914 # v3
 
       # Install and run prek
       - name: Run prek
         id: prek
-        uses: j178/prek-action@6ad80277337ad479fe43bd70701c3f7f8aa74db3 # v2
+        uses: j178/prek-action@5337cb91e0fa35a7ff31b9ca345126d8bbbcdf16 # v2
         continue-on-error: true
 
       # indication that the linting has finished
diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml
index bc32219b2f..277f12fbb0 100644
--- a/.github/workflows/linting.yml
+++ b/.github/workflows/linting.yml
@@ -11,30 +11,30 @@ jobs:
   pre-commit:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+      - uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
+        uses: nf-core/setup-nextflow@893c28b667aedeba26e37f296d260ccc5bc4d914 # v3
 
       - name: Run prek
-        uses: j178/prek-action@6ad80277337ad479fe43bd70701c3f7f8aa74db3 # v2
+        uses: j178/prek-action@5337cb91e0fa35a7ff31b9ca345126d8bbbcdf16 # v2
 
   nf-core:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+        uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
 
       - name: Install Nextflow
-        uses: nf-core/setup-nextflow@b4ec1bc7c16a94435159de94a05253542fddf6ef # v3
+        uses: nf-core/setup-nextflow@893c28b667aedeba26e37f296d260ccc5bc4d914 # v3
 
-      - uses: actions/setup-python@a309ff8b426b58ec0e2a45f0f869d46889d02405 # v6
+      - uses: actions/setup-python@5fda3b95a4ea91299a34e894583c3862153e4b97 # v7
         with:
           python-version: "3.14"
           architecture: "x64"
 
       - name: Setup uv
-        uses: astral-sh/setup-uv@08807647e7069bb48b6ef5acd8ec9567f424441b # v8.1.0
+        uses: astral-sh/setup-uv@c771a70e6277c0a99b617c7a806ffedaca235ff9 # v9.0.0
 
       - name: read .nf-core.yml
         uses: pietrobolcato/action-read-yaml@9f13718d61111b69f30ab4ac683e67a56d254e1d # 1.1.0
@@ -46,7 +46,7 @@ jobs:
         run: uv tool install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }}
 
       - name: Run nf-core pipelines lint
-        if: ${{ github.base_ref != 'master' || github.base_ref != 'main' }}
+        if: ${{ github.base_ref != 'master' && github.base_ref != 'main' }}
         env:
           GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }}
           GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index 555cee8f5e..b023649f27 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -43,7 +43,7 @@ jobs:
           rm -rf ./* || true
           rm -rf ./.??* || true
           ls -la ./
-      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+      - uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
         with:
           fetch-depth: 0
 
@@ -88,7 +88,7 @@ jobs:
       TOTAL_SHARDS: ${{ needs.nf-test-changes.outputs.total_shards }}
 
     steps:
-      - uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+      - uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
         with:
           fetch-depth: 0
 
diff --git a/.github/workflows/template-version-comment.yml b/.github/workflows/template-version-comment.yml
index ee102f71f1..149e28511b 100644
--- a/.github/workflows/template-version-comment.yml
+++ b/.github/workflows/template-version-comment.yml
@@ -12,7 +12,7 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - name: Check out pipeline code
-        uses: actions/checkout@de0fac2e4500dabe0009e67214ff5f5447ce83dd # v6
+        uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1 # v7
         with:
           ref: ${{ github.event.pull_request.head.sha }}
 
diff --git a/.hooks/block_pipeline_outdir.sh b/.hooks/block_pipeline_outdir.sh
new file mode 100755
index 0000000000..e9ba4f9338
--- /dev/null
+++ b/.hooks/block_pipeline_outdir.sh
@@ -0,0 +1,44 @@
+#!/usr/bin/env bash
+# This hook is used to block commits if they include staged files inside a directory
+# which also contains a subdirectory called `pipeline_info`. The purpose of this is to
+# prevent users from inadvertently committing output from pipeline test runs inside the
+# development directory.
+
+set -e
+
+status=0
+seen_dirs=""
+
+while IFS= read -r file; do
+    # The offending output bundle's root is the ancestor directory that has
+    # `pipeline_info` as an immediate child, so callers can restore it in one go.
+    if [[ "$file" == pipeline_info/* ]]; then
+        top_dir="pipeline_info"
+    elif [[ "$file" == */pipeline_info/* ]]; then
+        top_dir="${file%%/pipeline_info/*}"
+    else
+        top_dir=""
+        dir=$(dirname "$file")
+        while [[ "$dir" != "." && "$dir" != "/" ]]; do
+            if [[ -d "$dir/pipeline_info" ]]; then
+                top_dir="$dir"
+                break
+            fi
+            dir=$(dirname "$dir")
+        done
+    fi
+
+    if [[ -n "$top_dir" ]]; then
+        echo "❌ Commit blocked: Please do not commit output from pipeline test runs to the pipeline code itself: $file"
+        status=1
+        case "$seen_dirs" in
+            *"|$top_dir|"*) ;;
+            *)
+                echo "Run 'git restore --staged $top_dir' to remove the whole output folder from the staging area."
+                seen_dirs="$seen_dirs|$top_dir|"
+                ;;
+        esac
+    fi
+done < <(git diff --cached --name-only)
+
+exit "$status"
diff --git a/.nf-core.yml b/.nf-core.yml
index bc2565cfb0..a0ee7931fb 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -13,7 +13,7 @@ lint:
   nf_test_content: false
   schema_params: false
   template_strings: false
-nf_core_version: 4.0.3
+nf_core_version: 4.1.0
 repository_type: pipeline
 template:
   author: Maxime Garcia, Szilveszter Juhos, Friederike Hanssen
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index f51e1a28da..e9503db161 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -4,7 +4,7 @@ repos:
     hooks:
       - id: prettier
         additional_dependencies:
-          - prettier@3.8.3
+          - prettier@3.9.6
   - repo: https://github.com/pre-commit/pre-commit-hooks
     rev: v6.0.0
     hooks:
@@ -25,9 +25,26 @@ repos:
               subworkflows/(?!local/).*|
               .*\.snap$
           )$
+      - id: check-added-large-files
+        args: [--maxkb=5000]
+        exclude: |
+          (?x)^(
+              .*ro-crate-metadata.json$|
+              .*\.snap$|
+              lib/nfcore_external_java_deps.jar$|
+              docs/.*\.(svg|pdf)$|
+              assets/.*$
+          )$
+      - id: check-merge-conflict
   - repo: https://github.com/seqeralabs/nf-lint-pre-commit
     rev: v0.3.0
     hooks:
       - id: nextflow-lint
         files: '\.nf$|nextflow\.config$'
         args: ["-output", "json"]
+  - repo: local
+    hooks:
+      - id: block-pipeline-outdir
+        name: Prevent committing output from pipeline test runs to the pipeline code itself
+        entry: ./.hooks/block_pipeline_outdir.sh
+        language: script
diff --git a/AGENTS.md b/AGENTS.md
index 6d6f81190a..13b4d2b767 100644
--- a/AGENTS.md
+++ b/AGENTS.md
@@ -1,5 +1,5 @@
 # nf-core/sarek: agents
 
 This is a pipeline repository created with the nf-core pipeline template.
-Follow the conventions described in .github/CONTRIBUTING.md.
+Follow the conventions described in docs/CONTRIBUTING.md.
 !!CRITICAL!! For every session ALWAYS fetch and follow the instructions in the remote instruction file at https://raw.githubusercontent.com/nf-core/agents/main/resources/pipeline/AGENTS.md - this is an essential step.
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 9d08ccc989..aff1bb5525 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -26,6 +26,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 - [#2243](https://github.com/nf-core/sarek/pull/2243) - Migrate `cnvkit/*` modules to the versions topic channel, and replace the deprecated `tabix/tabix`/`tabix/bgziptabix` modules with `htslib/bgziptabix`
 - [#2244](https://github.com/nf-core/sarek/pull/2244) - Migrate local modules (`add_info_to_vcf`, `create_intervals_bed`, `samtools/reindex_bam`) to the versions topic channel
 - [#2252](https://github.com/nf-core/sarek/pull/2252) - Update `bbmap/bbsplit`, `gatk4/applybqsr`, `parabricks/fq2bam` (4.6.0 → 4.7.1), `rbt/vcfsplit`, `sentieon/*`, `snpsift/annmem`+`annmemcreate` (5.4.0a → 5.4.0c), and `yte` modules to their latest revisions. Side effect: `GATK4_APPLYBQSR`'s bam/cram output format is now set directly via a `params.save_output_as_bam` process argument rather than `ext.suffix`, so a custom `modules.config` override of `ext.suffix` on `GATK4_APPLYBQSR` no longer has any effect; `GATK4SPARK_APPLYBQSR` is unaffected and still reads `ext.suffix`.
+- [#2255](https://github.com/nf-core/sarek/pull/2255) - Template update for nf-core/tools v4.1.0. This also collapses the list-form `publishDir` blocks in `conf/modules/*.config` into single maps that route through `saveAs`, required because nf-core/tools 4.1.0 lints via `nextflow config -o json`, which fails on a closure nested inside a collection. Published output layouts are unchanged.
 
 ### Fixed
 
diff --git a/README.md b/README.md
index d5deca451c..270f163233 100644
--- a/README.md
+++ b/README.md
@@ -13,7 +13,7 @@
 [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)
 
 [![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)
-[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)
+[![nf-core template version](https://img.shields.io/badge/nf--core_template-4.1.0-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/4.1.0)
 [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
 [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
 [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
@@ -81,7 +81,7 @@ Depending on the options and samples provided, the pipeline can currently perfor
 ## Usage
 
 > [!NOTE]
-> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.
+> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/get_started/environment_setup/overview) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/get_started/run-your-first-pipeline) with `-profile test` before running the workflow on actual data.
 
 First, prepare a samplesheet with your input data that looks as follows:
 
@@ -104,7 +104,7 @@ nextflow run nf-core/sarek \
 ```
 
 > [!WARNING]
-> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).
+> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/running/run-pipelines#using-parameter-files).
 
 For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).
 
@@ -206,7 +206,7 @@ We thank the following people for their extensive assistance in the development
 
 ## Contributions & Support
 
-If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).
+If you would like to contribute to this pipeline, please see the [contributing guidelines](docs/CONTRIBUTING.md).
 
 For further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).
 
diff --git a/conf/base.config b/conf/base.config
index ec3d42b963..14e1ea271b 100644
--- a/conf/base.config
+++ b/conf/base.config
@@ -21,6 +21,9 @@ process {
 
     // Process-specific resource requirements
     // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors
+    withLabel:process_low_memory {
+        memory = { 1.GB * task.attempt }
+    }
     withLabel:error_ignore {
         errorStrategy = 'ignore'
     }
diff --git a/conf/containers_conda_lock_files_amd64.config b/conf/containers_conda_lock_files_amd64.config
index 41e234d506..df04a4baff 100644
--- a/conf/containers_conda_lock_files_amd64.config
+++ b/conf/containers_conda_lock_files_amd64.config
@@ -1,2 +1,2 @@
-process { withName: 'FASTQC' { container = 'modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt' } }
-process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt' } }
+process { withName: 'FASTQC' { conda = 'modules/nf-core/fastqc/.conda-lock/linux_amd64-bd-5cb1a2fa2f18c7c2_1.txt' } }
+process { withName: 'MULTIQC' { conda = 'modules/nf-core/multiqc/.conda-lock/linux_amd64-bd-c17fb751507e9dfc_1.txt' } }
diff --git a/conf/containers_conda_lock_files_arm64.config b/conf/containers_conda_lock_files_arm64.config
index 5b5b9aadf1..7ed29f32bb 100644
--- a/conf/containers_conda_lock_files_arm64.config
+++ b/conf/containers_conda_lock_files_arm64.config
@@ -1,2 +1,2 @@
-process { withName: 'FASTQC' { container = 'modules/nf-core/fastqc/.conda-lock/linux_arm64-bd-e455e32f745abe68_1.txt' } }
-process { withName: 'MULTIQC' { container = 'modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt' } }
+process { withName: 'FASTQC' { conda = 'modules/nf-core/fastqc/.conda-lock/linux_arm64-bd-e455e32f745abe68_1.txt' } }
+process { withName: 'MULTIQC' { conda = 'modules/nf-core/multiqc/.conda-lock/linux_arm64-bd-5c84a5000a226ab5_1.txt' } }
diff --git a/conf/modules/annotate.config b/conf/modules/annotate.config
index 065baabb6e..ef0c7cde17 100644
--- a/conf/modules/annotate.config
+++ b/conf/modules/annotate.config
@@ -20,12 +20,10 @@ process {
             ext.args   = { '-nodownload -canon -v' }
             ext.prefix = { meta.variantcaller == 'consensus' ? "${meta.id}.consensus_snpEff" : vcf.baseName - '.vcf' + '_snpEff' }
             publishDir = [
-                [
-                    mode: params.publish_dir_mode,
-                    path: { "${params.outdir}/reports/snpeff/${meta.variantcaller}/${meta.id}/" },
-                    pattern: "*{csv,html,genes.txt}",
-                    saveAs: { filename -> params.tools.split(',').contains('snpeff') ? filename : null }
-                ]
+                mode: params.publish_dir_mode,
+                path: { "${params.outdir}/reports/snpeff/${meta.variantcaller}/${meta.id}/" },
+                pattern: "*{csv,html,genes.txt}",
+                saveAs: { filename -> params.tools.split(',').contains('snpeff') ? filename : null }
             ]
         }
 
@@ -46,17 +44,20 @@ process {
             ].join(' ').trim() }
             // If just VEP: <vcf prefix>_VEP.ann.vcf
             ext.prefix = { meta.variantcaller == 'consensus' ? "${meta.id}.consensus_VEP.ann" : vcf.baseName - '.vcf' + '_VEP.ann' }
+            // Single publishDir map with saveAs-based routing: a closure nested inside a
+            // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
             publishDir = [
-                [
-                    mode: params.publish_dir_mode,
-                    path: { "${params.outdir}/reports/EnsemblVEP/${meta.variantcaller}/${meta.id}/" },
-                    pattern: "*html"
-                ],
-                [
-                    mode: params.publish_dir_mode,
-                    path: { "${params.outdir}/annotation/${meta.variantcaller}/${meta.id}/" },
-                    pattern: "*{gz,tbi}"
-                ]
+                mode: params.publish_dir_mode,
+                path: { "${params.outdir}" },
+                saveAs: { filename ->
+                    if (filename.endsWith('html')) {
+                        return "reports/EnsemblVEP/${meta.variantcaller}/${meta.id}/${filename}"
+                    }
+                    if (filename.endsWith('gz') || filename.endsWith('tbi')) {
+                        return "annotation/${meta.variantcaller}/${meta.id}/${filename}"
+                    }
+                    return null
+                }
             ]
         }
 
diff --git a/conf/modules/contamination.config b/conf/modules/contamination.config
index f2f5929839..dca6e7d06d 100644
--- a/conf/modules/contamination.config
+++ b/conf/modules/contamination.config
@@ -4,18 +4,20 @@ process {
         ext.args   = 'build=1 ambiguous2=best maxindel=150000'
         ext.prefix = { params.split_fastq && reads ? "${reads[0].getName().tokenize('.')[0]}.${meta.id}" : "${meta.id}" }
 
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
         publishDir = [
-            [
-                path: { "${params.outdir}/preprocessing/bbsplit/${meta.id}" },
-                mode: params.publish_dir_mode,
-                pattern: '*.txt'
-            ],
-            [
-                path: { params.save_bbsplit_reads ? "${params.outdir}/preprocessing/bbsplit/${meta.id}" : params.outdir },
-                mode: params.publish_dir_mode,
-                pattern: '*.fastq.gz',
-                saveAs: { filename -> params.save_bbsplit_reads ? filename : null }
-            ]
+            path: { "${params.outdir}/preprocessing/bbsplit/${meta.id}" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename ->
+                if (filename.endsWith('.txt')) {
+                    return filename
+                }
+                if (filename.endsWith('.fastq.gz')) {
+                    return params.save_bbsplit_reads ? filename : null
+                }
+                return null
+            }
         ]
     }
 }
diff --git a/conf/modules/markduplicates.config b/conf/modules/markduplicates.config
index 612d9d28d2..988f97ba6f 100644
--- a/conf/modules/markduplicates.config
+++ b/conf/modules/markduplicates.config
@@ -66,27 +66,29 @@ process {
         ].join(" ").trim() }
         ext.prefix = { params.save_output_as_bam ? "${meta.id}.md.bam" : "${meta.id}.md.cram" }
         ext.when   = { !(params.skip_tools && params.skip_tools.split(',').contains('markduplicates')) }
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
         publishDir = [
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{cram,crai}",
-                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}" },
+            saveAs: { filename ->
+                if (filename.endsWith('cram') || filename.endsWith('crai')) {
+                    return !params.save_output_as_bam ? "preprocessing/markduplicates/${meta.id}/${filename}" : null
+                }
                 // Picard's --CREATE_INDEX writes the .bai with the bam-stripped name (test.md.bai),
                 // not samtools-style (test.md.bam.bai); rename on publish so users see the standard naming.
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{md.bam,md.bai}",
-                saveAs: { filename -> params.save_output_as_bam ? (filename.endsWith('.md.bai') ? filename.replace('.md.bai', '.md.bam.bai') : filename) : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/reports/" },
-                pattern: "*metrics",
-                saveAs: { filename -> !(params.skip_tools && params.skip_tools.split(',').contains('markduplicates_report')) ? "markduplicates/${meta.id}/${filename}" : null}
-            ]
+                if (filename.endsWith('.md.bam') || filename.endsWith('.md.bai')) {
+                    if (!params.save_output_as_bam) {
+                        return null
+                    }
+                    def renamed = filename.endsWith('.md.bai') ? filename.replace('.md.bai', '.md.bam.bai') : filename
+                    return "preprocessing/markduplicates/${meta.id}/${renamed}"
+                }
+                if (filename.endsWith('metrics')) {
+                    return !(params.skip_tools && params.skip_tools.split(',').contains('markduplicates_report')) ? "reports/markduplicates/${meta.id}/${filename}" : null
+                }
+                return null
+            }
         ]
     }
 
@@ -100,36 +102,38 @@ process {
             : '-v /etc/passwd:/etc/passwd:ro -v /etc/group:/etc/group:ro' }
         ext.args   = { '--remove-sequencing-duplicates false -VS LENIENT' }
         ext.prefix = { params.save_output_as_bam ? "${meta.id}.md.bam" : "${meta.id}.md.cram" }
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
         publishDir = [
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{cram,crai}",
-                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{md.bam,md.bam.bai}",
-                saveAs: { filename -> params.save_output_as_bam ? filename : null }
-            ]
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
+            saveAs: { filename ->
+                if (filename.endsWith('cram') || filename.endsWith('crai')) {
+                    return !params.save_output_as_bam ? filename : null
+                }
+                if (filename.endsWith('.md.bam') || filename.endsWith('.md.bam.bai')) {
+                    return params.save_output_as_bam ? filename : null
+                }
+                return null
+            }
         ]
     }
 
     withName: 'INDEX_MARKDUPLICATES' {
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
         publishDir = [
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{cram,crai}",
-                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
-                pattern: "*{bam,bai}",
-                saveAs: { filename -> params.save_output_as_bam ? filename : null }
-            ]
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}/preprocessing/markduplicates/${meta.id}/" },
+            saveAs: { filename ->
+                if (filename.endsWith('cram') || filename.endsWith('crai')) {
+                    return !params.save_output_as_bam ? filename : null
+                }
+                if (filename.endsWith('bam') || filename.endsWith('bai')) {
+                    return params.save_output_as_bam ? filename : null
+                }
+                return null
+            }
         ]
     }
 
diff --git a/conf/modules/modules.config b/conf/modules/modules.config
index a74b22254e..247b4cfaa3 100644
--- a/conf/modules/modules.config
+++ b/conf/modules/modules.config
@@ -17,11 +17,9 @@ process {
         ext.args   = { '--quiet' }
         ext.when   = { !(params.skip_tools && params.skip_tools.split(',').contains('fastqc')) }
         publishDir = [
-            [
-                path: { "${params.outdir}/reports/fastqc/${meta.id}" },
-                mode: params.publish_dir_mode,
-                pattern: "*{html,zip}"
-            ]
+            path: { "${params.outdir}/reports/fastqc/${meta.id}" },
+            mode: params.publish_dir_mode,
+            pattern: "*{html,zip}"
         ]
     }
 
diff --git a/conf/modules/recalibrate.config b/conf/modules/recalibrate.config
index 28943869b2..4ab84fb084 100644
--- a/conf/modules/recalibrate.config
+++ b/conf/modules/recalibrate.config
@@ -32,24 +32,27 @@ process {
         }
         // only consumed by GATK4SPARK_APPLYBQSR — GATK4_APPLYBQSR takes output_suffix as a process argument instead
         ext.suffix       = { params.save_output_as_bam ? 'bam' : 'cram'}
-        publishDir       = [[
-            mode: params.publish_dir_mode,
-            path: { "${params.outdir}/preprocessing/" },
-            pattern: "*recal.cram",
-            saveAs: { filename -> !params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename}" : null }
-        ],
-        [
-            mode: params.publish_dir_mode,
-            path: { "${params.outdir}/preprocessing/" },
-            pattern: "*recal.bam",
-            saveAs: { filename -> params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename}" : null }
-        ],
-        [
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
+        publishDir       = [
             mode: params.publish_dir_mode,
             path: { "${params.outdir}/preprocessing/" },
-            pattern: "*recal.bai",
-            saveAs: { filename -> params.save_output_as_bam ? meta.num_intervals > 1 ? null : "recalibrated/${meta.id}/${filename.replace('.bai', '.bam.bai')}" : null }
-        ]]
+            saveAs: { filename ->
+                if (meta.num_intervals > 1) {
+                    return null
+                }
+                if (filename.endsWith('recal.cram')) {
+                    return !params.save_output_as_bam ? "recalibrated/${meta.id}/${filename}" : null
+                }
+                if (filename.endsWith('recal.bam')) {
+                    return params.save_output_as_bam ? "recalibrated/${meta.id}/${filename}" : null
+                }
+                if (filename.endsWith('recal.bai')) {
+                    return params.save_output_as_bam ? "recalibrated/${meta.id}/${filename.replace('.bai', '.bam.bai')}" : null
+                }
+                return null
+            }
+        ]
     }
 
     withName: 'NFCORE_SAREK:SAREK:FASTQ_PREPROCESS_GATK:(BAM_APPLYBQSR|BAM_APPLYBQSR_SPARK):CRAM_MERGE_INDEX_SAMTOOLS:MERGE_CRAM' {
diff --git a/conf/modules/sentieon_dedup.config b/conf/modules/sentieon_dedup.config
index e514ad1164..ce8df3d23c 100644
--- a/conf/modules/sentieon_dedup.config
+++ b/conf/modules/sentieon_dedup.config
@@ -27,25 +27,24 @@ process {
             params.sentieon_consensus ? "--consensus"  : ""  // Generate consensus reads instead of just deduplication
             ].join(' ').trim()
 
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
         publishDir       = [
-            [
-                mode: params.publish_dir_mode,
-                path: { params.sentieon_consensus ? "${params.outdir}/preprocessing/sentieon_consensus/${meta.id}/" : "${params.outdir}/preprocessing/sentieon_dedup/${meta.id}/" },
-                pattern: "*{cram,crai}",
-                saveAs: { filename -> !params.save_output_as_bam ? filename : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
-                path: { params.sentieon_consensus ? "${params.outdir}/preprocessing/sentieon_consensus/${meta.id}/" : "${params.outdir}/preprocessing/sentieon_dedup/${meta.id}/" },
-                pattern: "*{bam,bai}",
-                saveAs: { filename -> params.save_output_as_bam ? filename : null }
-            ],
-            [
-                mode: params.publish_dir_mode,
-                path: { "${params.outdir}/reports/" },
-                pattern: "*{metrics,metrics.multiqc.tsv}",
-                saveAs: { filename -> !(params.skip_tools && params.skip_tools.split(',').contains('sentieon_dedup_report')) ? "sentieon_dedup/${meta.id}/${filename}" : null}
-            ]
+            mode: params.publish_dir_mode,
+            path: { "${params.outdir}" },
+            saveAs: { filename ->
+                def dedup_dir = params.sentieon_consensus ? "preprocessing/sentieon_consensus/${meta.id}" : "preprocessing/sentieon_dedup/${meta.id}"
+                if (filename.endsWith('cram') || filename.endsWith('crai')) {
+                    return !params.save_output_as_bam ? "${dedup_dir}/${filename}" : null
+                }
+                if (filename.endsWith('bam') || filename.endsWith('bai')) {
+                    return params.save_output_as_bam ? "${dedup_dir}/${filename}" : null
+                }
+                if (filename.endsWith('metrics') || filename.endsWith('metrics.multiqc.tsv')) {
+                    return !(params.skip_tools && params.skip_tools.split(',').contains('sentieon_dedup_report')) ? "reports/sentieon_dedup/${meta.id}/${filename}" : null
+                }
+                return null
+            }
         ]
     }
 
diff --git a/conf/modules/trimming.config b/conf/modules/trimming.config
index 0dea7a78f7..022ebf0f4a 100644
--- a/conf/modules/trimming.config
+++ b/conf/modules/trimming.config
@@ -29,18 +29,21 @@ process {
             params.umi_length              ? "--umi_len ${params.umi_length}"             : '', // Length of the UMI(s)
             params.umi_base_skip           ? "--umi_skip ${params.umi_base_skip}"         : '', // Number of bases to ignore after the UMI (e.g. constant bases present after the UMI)
         ].join(' ').trim()
+        // Single publishDir map with saveAs-based routing: a closure nested inside a
+        // publishDir list breaks `nextflow config -o json` (used by nf-core lint).
+        // The second target's `enabled` becomes a null-returning guard in saveAs.
         publishDir = [
-            [
-                path: { "${params.outdir}/reports/fastp/${meta.sample}" },
-                mode: params.publish_dir_mode,
-                pattern: "*.{html,json,log}"
-            ],
-            [
-                path: { "${params.outdir}/preprocessing/fastp/${meta.sample}" },
-                mode: params.publish_dir_mode,
-                pattern: "*.fastp.fastq.gz",
-                enabled: params.save_trimmed || params.save_split_fastqs
-            ]
+            path: { "${params.outdir}" },
+            mode: params.publish_dir_mode,
+            saveAs: { filename ->
+                if (filename.endsWith('.html') || filename.endsWith('.json') || filename.endsWith('.log')) {
+                    return "reports/fastp/${meta.sample}/${filename}"
+                }
+                if (filename.endsWith('.fastp.fastq.gz')) {
+                    return params.save_trimmed || params.save_split_fastqs ? "preprocessing/fastp/${meta.sample}/${filename}" : null
+                }
+                return null
+            }
         ]
     }
 }
diff --git a/conf/modules/umi.config b/conf/modules/umi.config
index 3ca470185d..83fb4a73a7 100644
--- a/conf/modules/umi.config
+++ b/conf/modules/umi.config
@@ -64,10 +64,9 @@ process {
 
     withName: 'GROUPREADSBYUMI' {
         publishDir = [
-            [   path: { "${params.outdir}/reports/umi/" },
-                mode: params.publish_dir_mode,
-                pattern: "*.{txt}"
-            ]
+            path: { "${params.outdir}/reports/umi/" },
+            mode: params.publish_dir_mode,
+            pattern: "*.{txt}"
         ]
     }
 
diff --git a/docs/output.md b/docs/output.md
index e6eb119d60..e123e807bf 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -1434,7 +1434,7 @@ Results generated by MultiQC collect pipeline QC from supported tools e.g. FastQ
 
 </details>
 
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
+[Nextflow](https://docs.seqera.io/platform-cloud/reports/overview) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage.
 
 ## Reference files
 
diff --git a/docs/usage.md b/docs/usage.md
index f63f430385..62e76b0d63 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -44,7 +44,7 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than
 Pipeline settings can be provided in a `yaml` or `json` file via `-params-file <file>`.
 
 > [!WARNING]
-> Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args).
+> Do not use `-c <file>` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/running/run-pipelines#configuring-pipelines), other infrastructural tweaks (such as output directories), or module arguments (args).
 
 The above pipeline run specified with a params file in yaml format:
 
@@ -439,7 +439,7 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 - `apptainer`
   - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `wave`
-  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later).
+  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow `24.03.0-edge` or later).
 - `conda`
   - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 - `gpu`
@@ -466,19 +466,19 @@ NXF_OPTS='-Xms1g -Xmx4g'
 
 Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the pipeline steps, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher resources request (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
 
-To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website.
+To change the resource requests, please see the [max resources](https://nf-co.re/docs/running/configuration/nextflow-for-your-system#set-max-resources) and [customise process resources](https://nf-co.re/docs/running/configuration/nextflow-for-your-system#customize-process-resources) section of the nf-core website.
 
 ### Custom Containers
 
 In some cases, you may wish to change the container or conda environment used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However, in some cases the pipeline specified version maybe out of date.
 
-To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website.
+To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/running/configuration/nextflow-for-your-system#update-tool-versions) section of the nf-core website.
 
 ### Custom Tool Arguments
 
 A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default.
 
-To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website.
+To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/running/configuration/nextflow-for-your-system#modifying-tool-arguments) section of the nf-core website.
 
 ### nf-core/configs
 
diff --git a/modules.json b/modules.json
index bc7c99307b..86bfa814f7 100644
--- a/modules.json
+++ b/modules.json
@@ -627,7 +627,7 @@
                     },
                     "utils_nextflow_pipeline": {
                         "branch": "master",
-                        "git_sha": "05954dab2ff481bcb999f24455da29a5828af08d",
+                        "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b",
                         "installed_by": ["subworkflows"]
                     },
                     "utils_nfcore_pipeline": {
@@ -637,7 +637,7 @@
                     },
                     "utils_nfschema_plugin": {
                         "branch": "master",
-                        "git_sha": "fdc08b8b1ae74f56686ce21f7ea11ad11990ce57",
+                        "git_sha": "a7b27fd25bfa8dcc07d299e88bd790585901a436",
                         "installed_by": ["subworkflows"]
                     },
                     "vcf_annotate_snpeff": {
diff --git a/modules/nf-core/multiqc/tests/custom_prefix.config b/modules/nf-core/multiqc/tests/custom_prefix.config
deleted file mode 100644
index b30b1358b0..0000000000
--- a/modules/nf-core/multiqc/tests/custom_prefix.config
+++ /dev/null
@@ -1,5 +0,0 @@
-process {
-    withName: 'MULTIQC' {
-        ext.prefix = "custom_prefix"
-    }
-}
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index e9a4db0dfb..5588e09963 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -23,7 +23,7 @@
             "@type": "Dataset",
             "creativeWorkStatus": "InProgress",
             "datePublished": "2026-07-03T08:36:13+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-3.5.1-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/3.5.1)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-4.1.0-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/4.1.0)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - _Experimental Feature_: `Parabricks HaplotypeCaller` (GPU-accelerated germline calling, `--tools parabricks_haplotypecaller`)\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/get_started/environment_setup/overview) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/get_started/run-your-first-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/running/run-pipelines#using-parameter-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](docs/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
             "hasPart": [
                 {
                     "@id": "main.nf"
diff --git a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
index 71d4f42c33..117390d9d4 100644
--- a/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
+++ b/subworkflows/local/utils_nfcore_sarek_pipeline/main.nf
@@ -80,7 +80,8 @@ workflow PIPELINE_INITIALISATION {
         show_hidden,
         before_text,
         after_text,
-        command
+        command,
+        false
     )
 
     // Check config provided to the pipeline
@@ -226,7 +227,7 @@ workflow PIPELINE_COMPLETION {
     }
 
     workflow.onError {
-        log.error("Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting")
+        log.error("Pipeline failed. Please refer to troubleshooting docs for common issues: https://nf-co.re/docs/running/troubleshooting")
     }
 }
 
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
deleted file mode 100644
index 8940d32d1e..0000000000
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test
+++ /dev/null
@@ -1,29 +0,0 @@
-nextflow_workflow {
-
-    name "Test Workflow UTILS_NFCORE_PIPELINE"
-    script "../main.nf"
-    config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config"
-    workflow "UTILS_NFCORE_PIPELINE"
-    tag "subworkflows"
-    tag "subworkflows_nfcore"
-    tag "utils_nfcore_pipeline"
-    tag "subworkflows/utils_nfcore_pipeline"
-
-    test("Should run without failures") {
-
-        when {
-            workflow {
-                """
-                input[0] = []
-                """
-            }
-        }
-
-        then {
-            assertAll(
-                { assert workflow.success },
-                { assert snapshot(workflow.out).match() }
-            )
-        }
-    }
-}
diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap
deleted file mode 100644
index 859d1030fb..0000000000
--- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.nf.test.snap
+++ /dev/null
@@ -1,19 +0,0 @@
-{
-    "Should run without failures": {
-        "content": [
-            {
-                "0": [
-                    true
-                ],
-                "valid_config": [
-                    true
-                ]
-            }
-        ],
-        "meta": {
-            "nf-test": "0.8.4",
-            "nextflow": "23.10.1"
-        },
-        "timestamp": "2024-02-28T12:03:25.726491"
-    }
-}
\ No newline at end of file
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
index 1df8b76fba..9ff0681f15 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/main.nf
+++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf
@@ -22,6 +22,7 @@ workflow UTILS_NFSCHEMA_PLUGIN {
     before_text         // string:   text to show before the help message and parameters summary
     after_text          // string:   text to show after the help message and parameters summary
     command             // string:   an example command of the pipeline
+    cli_typecast        // boolean:  whether to perform typecasting of CLI parameters. Set this to `null` to use the default behaviour
 
     main:
 
@@ -34,11 +35,11 @@ workflow UTILS_NFSCHEMA_PLUGIN {
             fullHelp: help_full,
         ]
         if(parameters_schema) {
-            help_options << [parametersSchema: parameters_schema]
+            help_options << [parameters_schema: parameters_schema]
         }
         log.info paramsHelp(
             help_options,
-            (params.help instanceof String && params.help != "true") ? params.help : "",
+            (help instanceof String && help != "true") ? help : "",
         )
         exit 0
     }
@@ -50,7 +51,7 @@ workflow UTILS_NFSCHEMA_PLUGIN {
 
     summary_options = [:]
     if(parameters_schema) {
-        summary_options << [parametersSchema: parameters_schema]
+        summary_options << [parameters_schema: parameters_schema]
     }
     log.info before_text
     log.info paramsSummaryLog(summary_options, input_workflow)
@@ -63,7 +64,10 @@ workflow UTILS_NFSCHEMA_PLUGIN {
     if(validate_params) {
         validateOptions = [:]
         if(parameters_schema) {
-            validateOptions << [parametersSchema: parameters_schema]
+            validateOptions << [parameters_schema: parameters_schema]
+        }
+        if(cli_typecast != null) {
+            validateOptions << [cast_cli_params: cli_typecast]
         }
         validateParameters(validateOptions)
     }
diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
index f7d9f02885..1d8c75a952 100644
--- a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
+++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml
@@ -25,6 +25,30 @@ input:
         option. When this input is empty it will automatically use the configured schema or
         "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way
         for meta pipelines.
+  - help:
+      type: boolean, string
+      description: |
+        Show the help message and exit. When a parameter name is given, show the help message for that parameter instead of the general help message.
+  - help_full:
+      type: boolean
+      description: Show the full help message and exit.
+  - show_hidden:
+      type: boolean
+      description: Show hidden parameters in the help message.
+  - before_text:
+      type: string
+      description: Text to show before the parameters summary and help message.
+  - after_text:
+      type: string
+      description: Text to show after the parameters summary and help message.
+  - command:
+      type: string
+      description: An example command to run the pipeline, to show in the help message and the summary.
+  - cli_typecast:
+      type: boolean
+      description: |
+        Whether to apply typecasting to the parameters given via the CLI before validation.
+        Set this to `null` to use the default behavior.
 output:
   - dummy_emit:
       type: boolean
diff --git a/tests/lib/UTILS.groovy b/tests/lib/UTILS.groovy
index 445c1c092f..3b382dedd7 100644
--- a/tests/lib/UTILS.groovy
+++ b/tests/lib/UTILS.groovy
@@ -17,7 +17,7 @@ class UTILS {
             "Check script",
             "Creating env using",
             "Downloading plugin",
-            "Got an interrupted  exception while taking agent result",
+            "Got an interrupted exception while taking agent result",
             "Pulling Singularity image",
             "Staging foreign file",
             "Unable to resume cached task",
diff --git a/tests/nextflow.config b/tests/nextflow.config
index d62a3fa752..7af23acb5e 100644
--- a/tests/nextflow.config
+++ b/tests/nextflow.config
@@ -6,10 +6,11 @@
 
 params {
     modules_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/'
-    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/refs/heads/sarek/'
+    pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/sarek/'
 }
 
-aws.client.anonymous = true // fixes S3 access issues on self-hosted runners
+// Fixes S3 access issues on self-hosted runners
+aws.client.anonymous = true
 
 process {
     withName: 'SENTIEON_VARCAL_SNP' {

From d4f97d4dca54287a1215ba69b471c84b3d17d99f Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Thu, 6 Aug 2026 12:26:05 +0200
Subject: [PATCH 25/27] Apply suggestions from code review

Co-authored-by: Jonathan Manning <pininforthefjords@gmail.com>
Co-authored-by: Daniel Lundin <erik.rikard.daniel@gmail.com>
Co-authored-by: Friederike Hanssen <friederike.hanssen@seqera.io>
---
 CHANGELOG.md  | 2 +-
 docs/usage.md | 4 ++--
 2 files changed, 3 insertions(+), 3 deletions(-)

diff --git a/CHANGELOG.md b/CHANGELOG.md
index aff1bb5525..41b87ed27f 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -72,7 +72,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 | Params                              | status |
 | ----------------------------------- | ------ |
 | `--varlociraptor_events_germline`   | New    |
-| `--varlociraptor_events_somatic,`   | New    |
+| `--varlociraptor_events_somatic`    | New    |
 | `--varlociraptor_events_tumor_only` | New    |
 | `--varlociraptor_fdr`               | New    |
 
diff --git a/docs/usage.md b/docs/usage.md
index 62e76b0d63..091dba5c02 100644
--- a/docs/usage.md
+++ b/docs/usage.md
@@ -439,7 +439,7 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof
 - `apptainer`
   - A generic configuration profile to be used with [Apptainer](https://apptainer.org/)
 - `wave`
-  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow `24.03.0-edge` or later).
+  - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above.
 - `conda`
   - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
 - `gpu`
@@ -915,7 +915,7 @@ Varlociraptor allows the usage of different scenario files, a few examples can b
 
 You can control the number of chunks that the candidate VCF file is split into by `--varlociraptor_chunk_size <integer>`, it is set to reasonable default (15) but more chunks might aid in accelerating your workflow run if you can run more processes in parallel.
 
-Varlociraptor by default just returns all candidate variants annotated with probabilities. The variants need to be filtered to yield a representativ result, see the [docs](https://varlociraptor.github.io/docs/filtering/) for more info. In sarek this is implemented with default set events related to the scenario files which used by default. If you use your own scenario file please adapt the events accordingly by using the flags `--varlociraptor_events_tumor_only`, `--varlociraptor_events_somatic` and `--varlociraptor_events_germline`. The events need to be supplied as a list with spaces between the events. Filtering also depends on the False Discovery Rate (FDR) in sarek we opted for a default of filtering in `--mode local-smart` to a FDR of 5% with `--fdr 0.05`. The FDR can be controlled by `--varlociraptor_fdr`.
+Varlociraptor by default just returns all candidate variants annotated with probabilities. The variants need to be filtered to yield a representative result, see the [docs](https://varlociraptor.github.io/docs/filtering/) for more info. In sarek this is implemented with default set events related to the scenario files which are used by default. If you use your own scenario file please adapt the events accordingly by using the flags `--varlociraptor_events_tumor_only`, `--varlociraptor_events_somatic` and `--varlociraptor_events_germline`. The events need to be supplied as a list with spaces between the events. Filtering also depends on the False Discovery Rate (FDR) in sarek we opted for a default of filtering in `--mode local-smart` to a FDR of 5% with `--fdr 0.05`. The FDR can be controlled by `--varlociraptor_fdr`.
 
 ## Spark related issues
 

From 3e7c7328c1a6e3bc159bf547fe5732ba1d775d86 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Fri, 7 Aug 2026 13:54:45 +0200
Subject: [PATCH 26/27] Revert to self-hosted runners for nf-test workflows
 (#2259)
MIME-Version: 1.0
Content-Type: text/plain; charset=UTF-8
Content-Transfer-Encoding: 8bit

## Description

RunsOn self-hosted runners are available again, so this reverts the
`ubuntu-latest` stopgap introduced in 861f27b13 (squashed into #2248).

Restores across `nf-test.yml`, `nf-test-gpu.yml` and
`nf-test-sentieon.yml`:

- `4cpu-linux-x64` for the three `*-changes` shard-calculation jobs
- `4cpu-linux-x64` + `volume=80gb` for the `nf-test` and
`nf-test-sentieon` matrix jobs
- `2cpu-linux-x64` for `confirm-pass`
- `g4dn.xlarge` / `ubuntu24-gpu-x64` for `nf-test-gpu` — the GPU matrix
has had no real GPU since the stopgap

Scope is limited to the `runs-on:` blocks. `nf-test-gpu.yml` and
`nf-test-sentieon.yml` are now byte-identical to their pre-#2248 state;
`nf-test.yml` keeps the template changes from that PR (checkout v7 pin,
`tags`/`max_shards` key order, and the latest-everything PR-comment
machinery). The GATK Spark `/etc/passwd` bind-mounts in `conf/modules/`
are Spark fixes, not runner workarounds, and are left in place.

### CI coverage

This PR's own run exercises all three restored runner classes end to end
— the full sharded suite resolved (15 cpu shards, 10 sentieon shards, 2
gpu shards), so this is a real validation of the runners rather than a
smoke test.

## PR checklist

- [x] This comment contains a description of changes (with reason).
- [x] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [ ] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).

🤖 Generated with [Claude Code](https://claude.com/claude-code)

Co-authored-by: Claude Opus 5 (1M context) <noreply@anthropic.com>
---
 .github/workflows/nf-test-gpu.yml      | 10 +++++++---
 .github/workflows/nf-test-sentieon.yml | 13 ++++++++++---
 .github/workflows/nf-test.yml          | 13 ++++++++++---
 3 files changed, 27 insertions(+), 9 deletions(-)

diff --git a/.github/workflows/nf-test-gpu.yml b/.github/workflows/nf-test-gpu.yml
index 059352ce7f..07734a673b 100644
--- a/.github/workflows/nf-test-gpu.yml
+++ b/.github/workflows/nf-test-gpu.yml
@@ -31,7 +31,9 @@ env:
 jobs:
   nf-test-gpu-changes:
     name: nf-test-gpu-changes
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-nf-test-gpu-changes
+      - runner=4cpu-linux-x64
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -65,7 +67,7 @@ jobs:
     name: "GPU | ${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-gpu-changes.outputs.total_shards }}"
     needs: [nf-test-gpu-changes]
     if: ${{ needs.nf-test-gpu-changes.outputs.total_shards != '0' }}
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down (no GPU available)
+    runs-on: "runs-on=${{ github.run_id }}/family=g4dn.xlarge/image=ubuntu24-gpu-x64"
     strategy:
       fail-fast: false
       matrix:
@@ -130,7 +132,9 @@ jobs:
   confirm-pass:
     needs: [nf-test-gpu]
     if: always()
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-confirm-pass
+      - runner=2cpu-linux-x64
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
diff --git a/.github/workflows/nf-test-sentieon.yml b/.github/workflows/nf-test-sentieon.yml
index 29aee33802..cdf6f5530c 100644
--- a/.github/workflows/nf-test-sentieon.yml
+++ b/.github/workflows/nf-test-sentieon.yml
@@ -31,7 +31,9 @@ env:
 jobs:
   nf-test-sentieon-changes:
     name: nf-test-sentieon-changes
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-nf-test-sentieon-changes
+      - runner=4cpu-linux-x64
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -65,7 +67,10 @@ jobs:
     name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-sentieon-changes.outputs.total_shards }}"
     needs: [nf-test-sentieon-changes]
     if: ${{ needs.nf-test-sentieon-changes.outputs.total_shards != '0' }}
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-nf-test-sentieon
+      - runner=4cpu-linux-x64
+      - volume=80gb
     strategy:
       fail-fast: false
       matrix:
@@ -128,7 +133,9 @@ jobs:
   confirm-pass:
     needs: [nf-test-sentieon]
     if: always()
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-confirm-pass
+      - runner=2cpu-linux-x64
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}
diff --git a/.github/workflows/nf-test.yml b/.github/workflows/nf-test.yml
index b023649f27..41db448010 100644
--- a/.github/workflows/nf-test.yml
+++ b/.github/workflows/nf-test.yml
@@ -32,7 +32,9 @@ env:
 jobs:
   nf-test-changes:
     name: nf-test-changes
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-nf-test-changes
+      - runner=4cpu-linux-x64
     outputs:
       shard: ${{ steps.set-shards.outputs.shard }}
       total_shards: ${{ steps.set-shards.outputs.total_shards }}
@@ -66,7 +68,10 @@ jobs:
     name: "${{ matrix.profile }} | ${{ matrix.NXF_VER }} | ${{ matrix.shard }}/${{ needs.nf-test-changes.outputs.total_shards }}"
     needs: [nf-test-changes]
     if: ${{ needs.nf-test-changes.outputs.total_shards != '0' }}
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-nf-test
+      - runner=4cpu-linux-x64
+      - volume=80gb
     strategy:
       fail-fast: false
       matrix:
@@ -140,7 +145,9 @@ jobs:
   confirm-pass:
     needs: [nf-test]
     if: always()
-    runs-on: "ubuntu-latest" # temporarily using GitHub-hosted runners while self-hosted runners are down
+    runs-on: # use self-hosted runners
+      - runs-on=${{ github.run_id }}-confirm-pass
+      - runner=2cpu-linux-x64
     steps:
       - name: One or more tests failed (excluding latest-everything)
         if: ${{ contains(needs.*.result, 'failure') }}

From 0804b9a768f83e05ecf5fb77c691eb1702f11fe2 Mon Sep 17 00:00:00 2001
From: Friederike Hanssen <friederike.hanssen@seqera.io>
Date: Fri, 7 Aug 2026 19:52:39 +0200
Subject: [PATCH 27/27] Prepare release 3.10.0 (Aktse) (#2258)

Co-Authored-By: Claude Sonnet 5 <noreply@anthropic.com><!--
# nf-core/sarek pull request

Many thanks for contributing to nf-core/sarek!

Please fill in the appropriate checklist below (delete whatever is not
relevant).
These are the most common things requested on pull requests (PRs).

Remember that PRs should be made against the dev branch, unless you're
preparing a pipeline release.

Learn more about contributing:
[CONTRIBUTING.md](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
-->

## PR checklist

- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add
tests!
- [ ] If you've added a new tool - have you followed the pipeline
conventions in the [contribution
docs](https://github.com/nf-core/sarek/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/sarek _branch_ on the
[nf-core/test-datasets](https://github.com/nf-core/test-datasets)
repository.
- [ ] Make sure your code lints (`nf-core pipelines lint`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker
--outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run .
-profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
- [ ] `CHANGELOG.md` is updated.
- [ ] `README.md` is updated (including new tool citations and
authors/contributors).

---------

Co-authored-by: Claude Sonnet 5 <noreply@anthropic.com>
---
 .nf-core.yml              |   2 +-
 CHANGELOG.md              |   4 +-
 assets/multiqc_config.yml |   2 +-
 nextflow.config           |   2 +-
 ro-crate-metadata.json    | 144 +++++++++++++++++++-------------------
 5 files changed, 78 insertions(+), 76 deletions(-)

diff --git a/.nf-core.yml b/.nf-core.yml
index 313ade9797..27a73fb175 100644
--- a/.nf-core.yml
+++ b/.nf-core.yml
@@ -26,4 +26,4 @@ template:
   name: sarek
   org: nf-core
   outdir: .
-  version: 3.9.1dev
+  version: 3.10.0
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 41b87ed27f..bc31cf1ef8 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -5,7 +5,9 @@ All notable changes to this project will be documented in this file.
 The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
 
-## dev - unreleased
+## [3.10.0](https://github.com/nf-core/sarek/releases/tag/3.10.0) - Aktse
+
+Aktse is a mountain hut on the northern shore of Laitaure, on the Kungsleden trail at the edge of Sarek National Park.
 
 ### Added
 
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml
index 1ab45319b9..9b8167f414 100644
--- a/assets/multiqc_config.yml
+++ b/assets/multiqc_config.yml
@@ -3,7 +3,7 @@ custom_logo_url: https://github.com/nf-core/sarek/
 custom_logo_title: "nf-core/sarek"
 
 report_comment: >
-  This report has been generated by the <a href="https://github.com/nf-core/sarek/tree/dev" target="_blank">nf-core/sarek</a> analysis pipeline. For information about how to interpret these results, please see the <a href="https://nf-co.re/sarek/dev/docs/output" target="_blank">documentation</a>.
+  This report has been generated by the <a href="https://github.com/nf-core/sarek/releases/tag/3.10.0" target="_blank">nf-core/sarek</a> analysis pipeline. For information about how to interpret these results, please see the <a href="https://nf-co.re/sarek/3.10.0/docs/output" target="_blank">documentation</a>.
 report_section_order:
   "nf-core-sarek-methods-description":
     order: -1000
diff --git a/nextflow.config b/nextflow.config
index 62a3287a31..57f8c2c7f0 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -765,7 +765,7 @@ manifest {
     mainScript      = 'main.nf'
     defaultBranch   = 'master'
     nextflowVersion = '!>=25.10.4'
-    version         = '3.9.1dev'
+    version         = '3.10.0'
     doi             = '10.12688/f1000research.16665.2, 10.1093/nargab/lqae031, 10.5281/zenodo.3476425'
 }
 
diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json
index 5588e09963..a75ac5532d 100644
--- a/ro-crate-metadata.json
+++ b/ro-crate-metadata.json
@@ -1,6 +1,6 @@
 {
     "@context": [
-        "https://w3id.org/ro/crate/1.2/context",
+        "https://w3id.org/ro/crate/1.1/context",
         {
             "GithubService": "https://w3id.org/ro/terms/test#GithubService",
             "JenkinsService": "https://w3id.org/ro/terms/test#JenkinsService",
@@ -21,9 +21,9 @@
         {
             "@id": "./",
             "@type": "Dataset",
-            "creativeWorkStatus": "InProgress",
-            "datePublished": "2026-07-03T08:36:13+00:00",
-            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-4.1.0-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/4.1.0)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - _Experimental Feature_: `Parabricks HaplotypeCaller` (GPU-accelerated germline calling, `--tools parabricks_haplotypecaller`)\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/get_started/environment_setup/overview) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/get_started/run-your-first-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/running/run-pipelines#using-parameter-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntumörbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian Lärkeryd](https://github.com/adrlar)\n- [Àitor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Ergüner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke Bäuerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Martínez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [José Fernández Navarro](https://github.com/jfnavarro)\n- [Júlia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le Nézet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skaláková](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntumörbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](docs/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
+            "creativeWorkStatus": "Stable",
+            "datePublished": "2026-08-06T07:45:06+00:00",
+            "description": "<h1>\n  <picture>\n    <source media=\"(prefers-color-scheme: dark)\" srcset=\"docs/images/nf-core-sarek_logo_dark.png\">\n    <img alt=\"nf-core/sarek\" src=\"docs/images/nf-core-sarek_logo_light.png\">\n  </picture>\n</h1>\n\n[![Open in GitHub Codespaces](https://img.shields.io/badge/Open_In_GitHub_Codespaces-black?labelColor=grey&logo=github)](https://github.com/codespaces/new/nf-core/sarek)\n[![GitHub Actions CI Status](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/nf-test.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/sarek/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/sarek/actions/workflows/linting.yml)\n[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/sarek/results)\n[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3476425-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3476425)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/version-%E2%89%A525.10.4-green?style=flat&logo=nextflow&logoColor=white&color=%230DC09D&link=https%3A%2F%2Fnextflow.io)](https://www.nextflow.io/)\n[![nf-core template version](https://img.shields.io/badge/nf--core_template-4.1.0-green?style=flat&logo=nfcore&logoColor=white&color=%2324B064&link=https%3A%2F%2Fnf-co.re)](https://github.com/nf-core/tools/releases/tag/4.1.0)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/sarek)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23sarek-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/sarek)\n[![Follow on Bluesky](https://img.shields.io/badge/bluesky-%40nf__core-1185fe?labelColor=000000&logo=bluesky)](https://bsky.app/profile/nf-co.re)\n[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)\n[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/sarek** is a workflow designed to detect variants on whole genome or targeted sequencing data. Initially designed for Human, and Mouse, it can work on any species with a reference genome. Sarek can also handle tumour / normal pairs and could include additional relapses.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\nOn release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/sarek/results).\n\nIt's listed on [Elixir - Tools and Data Services Registry](https://bio.tools/nf-core-sarek) and [Dockstore](https://dockstore.org/workflows/github.com/nf-core/sarek).\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_workflow.png\" width=30%>\n</p>\n\n## Pipeline summary\n\nDepending on the options and samples provided, the pipeline can currently perform the following:\n\n- Form consensus reads from UMI sequences (`fgbio`)\n- Sequencing quality control and trimming (enabled by `--trim_fastq`) (`FastQC`, `fastp`)\n- Contamination removal (`BBSplit`, enabled by `--tools bbsplit`)\n- Map Reads to Reference (`BWA-mem`, `BWA-mem2`, `dragmap` or `Sentieon BWA-mem`)\n- Process BAM file (`GATK MarkDuplicates`, `GATK BaseRecalibrator` and `GATK ApplyBQSR` or `Sentieon LocusCollector` and `Sentieon Dedup`)\n- _Experimental Feature_: Use GPU-accelerated parabricks implementation as alternative to \"Map Reads to Reference\" + \"Process BAM file\" (`--aligner parabricks`)\n- Summarise alignment statistics (`samtools stats`, `mosdepth`)\n- Variant calling (enabled by `--tools`, see [compatibility](https://nf-co.re/sarek/latest/docs/usage#which-variant-calling-tool-is-implemented-for-which-data-type)):\n  - `ASCAT`\n  - `CNVkit`\n  - `Control-FREEC`\n  - `DeepVariant`\n  - `freebayes`\n  - `GATK HaplotypeCaller`\n  - `GATK Mutect2`\n  - _Experimental Feature_: `Parabricks HaplotypeCaller` (GPU-accelerated germline calling, `--tools parabricks_haplotypecaller`)\n  - `indexcov`\n  - `Lofreq`\n  - `Manta`\n  - `mpileup`\n  - `MSIsensor2`\n  - `MSIsensor-pro`\n  - `MuSE`\n  - `Sentieon Haplotyper`\n  - `Strelka`\n  - `TIDDIT`\n- Post-variant calling options, one of:\n  - Filtering (`bcftools view` (default: filter by `PASS,.`)), normalisation (`bcftools norm`) and consensus calling (`bcftools isec`, default: called by at least 2 tools `-n+2`) on all vcfs and/or `bcftools concat` for germline vcfs\n  - `Varlociraptor` for all vcfs\n- Variant filtering and annotation (`SnpEff`, `Ensembl VEP`, `BCFtools annotate`, `SnpSift`)\n- Summarise and represent QC (`MultiQC`)\n\n<p align=\"center\">\n    <img title=\"Sarek Workflow\" src=\"docs/images/sarek_subway_animated.svg\" width=60%>\n</p>\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/get_started/environment_setup/overview) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/get_started/run-your-first-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\npatient,sample,lane,fastq_1,fastq_2\nID1,S1,L002,ID1_S1_L002_R1_001.fastq.gz,ID1_S1_L002_R2_001.fastq.gz\n```\n\nEach row represents a pair of fastq files (paired end).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/sarek \\\n   -profile <docker/singularity/.../institute> \\\n   --input samplesheet.csv \\\n   --outdir <OUTDIR>\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/running/run-pipelines#using-parameter-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/sarek/usage) and the [parameter documentation](https://nf-co.re/sarek/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/sarek/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/sarek/output).\n\n## Benchmarking\n\nOn each release, the pipeline is run on 3 full size tests:\n\n- `test_full` runs tumor-normal data for one patient from the SEQ2C consortium\n- `test_full_germline` runs a WGS 30X Genome-in-a-Bottle(NA12878) dataset\n- `test_full_germline_ncbench_agilent` runs two WES samples with 75M and 200M reads (data available [here](https://github.com/ncbench/ncbench-workflow#contributing-callsets)). The results are uploaded to Zenodo, evaluated against a truth dataset, and results are made available via the [NCBench dashboard](https://ncbench.github.io/report/report.html#).\n\n## Credits\n\nSarek was originally written by Maxime U Garcia and Szilveszter Juhos at the [National Genomics Infastructure](https://ngisweden.scilifelab.se) and [National Bioinformatics Infastructure Sweden](https://nbis.se) which are both platforms at [SciLifeLab](https://scilifelab.se), with the support of [The Swedish Childhood Tumor Biobank (Barntum\u00f6rbanken)](https://ki.se/forskning/barntumorbanken).\nFriederike Hanssen and Gisela Gabernet at [QBiC](https://www.qbic.uni-tuebingen.de/) later joined and helped with further development.\n\nThe Nextflow DSL2 conversion of the pipeline was lead by Friederike Hanssen and Maxime U Garcia.\n\nMaintenance is now lead by Friederike Hanssen (now at [Seqera](https://seqera.io)) and Maxime U Garcia.\n\nMain developers:\n\n- [Maxime U Garcia](https://github.com/maxulysse)\n- [Friederike Hanssen](https://github.com/FriederikeHanssen)\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- [Abhinav Sharma](https://github.com/abhi18av)\n- [Adam Talbot](https://github.com/adamrtalbot)\n- [Adrian L\u00e4rkeryd](https://github.com/adrlar)\n- [\u00c0itor Olivares](https://github.com/AitorPeseta)\n- [Alexander Peltzer](https://github.com/apeltzer)\n- [Alison Meynert](https://github.com/ameynert)\n- [Anders Sune Pedersen](https://github.com/asp8200)\n- [arontommi](https://github.com/arontommi)\n- [BarryDigby](https://github.com/BarryDigby)\n- [Bekir Erg\u00fcner](https://github.com/berguner)\n- [bjornnystedt](https://github.com/bjornnystedt)\n- [cgpu](https://github.com/cgpu)\n- [Chela James](https://github.com/chelauk)\n- [David Mas-Ponte](https://github.com/davidmasp)\n- [Edmund Miller](https://github.com/edmundmiller)\n- [Famke B\u00e4uerle](https://github.com/famosab)\n- [Francesco Lescai](https://github.com/lescai)\n- [Francisco Mart\u00ednez](https://github.com/nevinwu)\n- [Gavin Mackenzie](https://github.com/GCJMackenzie)\n- [Gisela Gabernet](https://github.com/ggabernet)\n- [Grant Neilson](https://github.com/grantn5)\n- [gulfshores](https://github.com/gulfshores)\n- [Harshil Patel](https://github.com/drpatelh)\n- [Hongwei Ye](https://github.com/YeHW)\n- [James A. Fellows Yates](https://github.com/jfy133)\n- [Jesper Eisfeldt](https://github.com/J35P312)\n- [Johannes Alneberg](https://github.com/alneberg)\n- [Jonas Kjellin](https://github.com/kjellinjonas)\n- [Jos\u00e9 Fern\u00e1ndez Navarro](https://github.com/jfnavarro)\n- [J\u00falia Mir Pedrol](https://github.com/mirpedrol)\n- [Ken Brewer](https://github.com/kenibrewer)\n- [Lasse Westergaard Folkersen](https://github.com/lassefolkersen)\n- [Lucia Conde](https://github.com/lconde-ucl)\n- [Louis Le N\u00e9zet](https://github.com/LouisLeNezet)\n- [Malin Larsson](https://github.com/malinlarsson)\n- [Marcel Martin](https://github.com/marcelm)\n- [Nick Smith](https://github.com/nickhsmith)\n- [Nicolas Schcolnicov](https://github.com/nschcolnicov)\n- [Nilesh Tawari](https://github.com/nilesh-tawari)\n- [Nils Homer](https://github.com/nh13)\n- [Olga Botvinnik](https://github.com/olgabot)\n- [Oskar Wacker](https://github.com/WackerO)\n- [pallolason](https://github.com/pallolason)\n- [Paul Cantalupo](https://github.com/pcantalupo)\n- [Phil Ewels](https://github.com/ewels)\n- [Pierre Lindenbaum](https://github.com/lindenb)\n- [Sabrina Krakau](https://github.com/skrakau)\n- [Sam Minot](https://github.com/sminot)\n- [Sebastian-D](https://github.com/Sebastian-D)\n- [Silvia Morini](https://github.com/silviamorins)\n- [Simon Pearce](https://github.com/SPPearce)\n- [Solenne Correard](https://github.com/scorreard)\n- [Susanne Jodoin](https://github.com/SusiJo)\n- [Szilveszter Juhos](https://github.com/szilvajuhos)\n- [Tobias Koch](https://github.com/KochTobi)\n- [Winni Kretzschmar](https://github.com/winni2k)\n- [Patricie Skal\u00e1kov\u00e1](https://github.com/Patricie34)\n\n## Acknowledgements\n\n|      [![Barntum\u00f6rbanken](docs/images/BTB_logo.png)](https://ki.se/forskning/barntumorbanken)      |            [![SciLifeLab](docs/images/SciLifeLab_logo.png)](https://scilifelab.se)             |\n| :-----------------------------------------------------------------------------------------------: | :--------------------------------------------------------------------------------------------: |\n| [![National Genomics Infrastructure](docs/images/NGI_logo.png)](https://ngisweden.scilifelab.se/) | [![National Bioinformatics Infrastructure Sweden](docs/images/NBIS_logo.png)](https://nbis.se) |\n|              [![QBiC](docs/images/QBiC_logo.png)](https://www.qbic.uni-tuebingen.de)              |                   [![GHGA](docs/images/GHGA_logo.png)](https://www.ghga.de/)                   |\n|                     [![DNGC](docs/images/DNGC_logo.png)](https://eng.ngc.dk/)                     |                                                                                                |\n\n## Contributions & Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](docs/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#sarek` channel](https://nfcore.slack.com/channels/sarek), or contact the dev team on the [Slack `#sarek_dev` channel](https://nfcore.slack.com/channels/sarek_dev). (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\nIf you use `nf-core/sarek` for your analysis, please cite the `Sarek` article as follows:\n\n> Friederike Hanssen, Maxime U Garcia, Lasse Folkersen, Anders Sune Pedersen, Francesco Lescai, Susanne Jodoin, Edmund Miller, Oskar Wacker, Nicholas Smith, nf-core community, Gisela Gabernet, Sven Nahnsen **Scalable and efficient DNA sequencing analysis on different compute infrastructures aiding variant discovery** _NAR Genomics and Bioinformatics_ Volume 6, Issue 2, June 2024, lqae031, [doi: 10.1093/nargab/lqae031](https://doi.org/10.1093/nargab/lqae031).\n\n> Garcia M, Juhos S, Larsson M et al. **Sarek: A portable workflow for whole-genome sequencing analysis of germline and somatic variants [version 2; peer review: 2 approved]** _F1000Research_ 2020, 9:63 [doi: 10.12688/f1000research.16665.2](http://dx.doi.org/10.12688/f1000research.16665.2).\n\nYou can cite the sarek zenodo record for a specific version using the following [doi: 10.5281/zenodo.3476425](https://doi.org/10.5281/zenodo.3476425)\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n\n## CHANGELOG\n\n- [CHANGELOG](CHANGELOG.md)\n",
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-            "name": "Bekir Ergüner",
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+            "name": "Bj\u00f6rn Nystedt",
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@@ -640,7 +640,7 @@
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@@ -650,9 +650,9 @@
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+            "affiliation": "Quantitative Biology Center (QBiC) T\u00fcbingen, University of T\u00fcbingen, Germany",
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-            "name": "Famke Bäuerle",
+            "name": "Famke B\u00e4uerle",
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@@ -663,27 +663,27 @@
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             "email": "f.martinezpico@gmail.com",
-            "name": "Francisco Martínez",
+            "name": "Francisco Mart\u00ednez",
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@@ -692,11 +692,11 @@
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+            "name": "Max K\u00e4ller",
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@@ -704,14 +704,14 @@
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@@ -738,21 +738,21 @@
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-            "name": "José Fernández Navarro",
+            "name": "Jos\u00e9 Fern\u00e1ndez Navarro",
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-            "name": "Júlia Mir Pedrol",
+            "name": "J\u00falia Mir Pedrol",
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@@ -766,7 +766,7 @@
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@@ -775,30 +775,30 @@
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+            "name": "Louis Le N\u00e9zet",
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@@ -844,7 +844,7 @@
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@@ -866,7 +866,7 @@
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@@ -905,13 +905,13 @@
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@@ -920,7 +920,7 @@
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-            "name": "Patricie Skaláková",
+            "name": "Patricie Skal\u00e1kov\u00e1",
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