I was trying to map gene identifiers from Entrez gene ID to HGNC symbols using the following code:
## prep
if (!requireNamespace("BiocManager", quietly = TRUE)) {
install.packages("BiocManager")
BiocManager::install()
}
if (!requireNamespace("BridgeDbR", quietly = TRUE))
BiocManager::install("BridgeDbR", ask = FALSE)
normalised_counts <- readRDS(file = "./normalised_counts.rds")
## BridgeDbR mapping ============================================
entrezID <- rownames(normalised_counts)
dbname <- BridgeDbR::getBridgeNames(code = "Hs")
## file path to Bridge database
dbLocation <- file.path(".", "data", dbname)
## download only if it hasn't been downloaded
if (!file.exists(dbLocation)) {
dbLocation <- BridgeDbR::getDatabase(organism = "Homo sapiens",
location = file.path(".", "data"))
}
mapper <- BridgeDbR::loadDatabase(dbLocation)
input <- data.frame(
source = rep(BridgeDbR::getSystemCode("Entrez Gene"), length(entrezID)),
identifer = entrezID
)
entrez2Hgnc_bridge <- BridgeDbR::maps(
mapper = mapper,
identifiers = input,
target = BridgeDbR::getSystemCode("HGNC")
)
The maps function threw java.lang.NoSuchMethodError. Traceback info:
Error in map(mapper, source = source, identifier = identifier, target = target) : java.lang.NoSuchMethodError: <init>
4. stop(structure(list(message = "java.lang.NoSuchMethodError: <init>", call = map(mapper, source = source, identifier = identifier, target = target), jobj = new("jobjRef", jobj = <pointer: 0x000002383ce07518>, jclass = "java/lang/NoSuchMethodError")), class = c("NoSuchMethodError", ...
3. .jnew("org/bridgedb/Xref", identifier, datasource)
2. map(mapper, source = source, identifier = identifier, target = target)
1. BridgeDbR::maps(mapper = mapper, identifiers = input, target = "H")
I tested this on two separate machines, one running R version 4.0.4 with Bioconductor 3.12 and the other running R 4.1.0 with Bioconductor 3.13. maps() threw the same error on both machines. Oddly, this code snippet worked back in February with no issues. I'm not sure if this error has to do with the Java version. As for the sessioninfo() and the Java versions of the two machines I tested on:
## machine 1
> sessionInfo()
R version 4.1.0 (2021-05-18)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19042)
Matrix products: default
locale:
[1] LC_COLLATE=Chinese (Simplified)_China.936 LC_CTYPE=Chinese (Simplified)_China.936
[3] LC_MONETARY=Chinese (Simplified)_China.936 LC_NUMERIC=C
[5] LC_TIME=Chinese (Simplified)_China.936
attached base packages:
[1] parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] BridgeDbR_2.2.1 rJava_1.0-4 kableExtra_1.3.4 GEOquery_2.60.0 Biobase_2.52.0
[6] BiocGenerics_0.38.0 edgeR_3.34.0 limma_3.48.0
loaded via a namespace (and not attached):
[1] bitops_1.0-7 lubridate_1.7.10 bit64_4.0.5 filelock_1.0.2
[5] webshot_0.5.2 progress_1.2.2 httr_1.4.2 rprojroot_2.0.2
[9] GenomeInfoDb_1.28.1 tools_4.1.0 utf8_1.2.1 R6_2.5.0
[13] DBI_1.1.1 colorspace_2.0-2 withr_2.4.2 tidyselect_1.1.1
[17] prettyunits_1.1.1 downlit_0.2.1 bit_4.0.4 curl_4.3.2
[21] compiler_4.1.0 cli_3.0.0 rvest_1.0.0 xml2_1.3.2
[25] scales_1.1.1 readr_1.4.0 rappdirs_0.3.3 systemfonts_1.0.2
[29] stringr_1.4.0 digest_0.6.27 rmarkdown_2.9 svglite_2.0.0
[33] XVector_0.32.0 pkgconfig_2.0.3 htmltools_0.5.1.1 highr_0.9
[37] dbplyr_2.1.1 fastmap_1.1.0 rlang_0.4.11 rstudioapi_0.13
[41] RSQLite_2.2.7 generics_0.1.0 jsonlite_1.7.2 dplyr_1.0.7
[45] distill_1.2.4 RCurl_1.98-1.3 magrittr_2.0.1 GenomeInfoDbData_1.2.6
[49] Rcpp_1.0.6 munsell_0.5.0 S4Vectors_0.30.0 fansi_0.5.0
[53] lifecycle_1.0.0 stringi_1.6.2 GEOmetadb_1.54.0 yaml_2.2.1
[57] zlibbioc_1.38.0 BiocFileCache_2.0.0 grid_4.1.0 blob_1.2.1
[61] crayon_1.4.1 lattice_0.20-44 Biostrings_2.60.1 annotate_1.70.0
[65] hms_1.1.0 KEGGREST_1.32.0 locfit_1.5-9.4 knitr_1.33
[69] pillar_1.6.1 biomaRt_2.48.2 stats4_4.1.0 XML_3.99-0.6
[73] glue_1.4.2 evaluate_0.14 BiocManager_1.30.16 png_0.1-7
[77] vctrs_0.3.8 purrr_0.3.4 tidyr_1.1.3 assertthat_0.2.1
[81] cachem_1.0.5 xfun_0.23 xtable_1.8-4 viridisLite_0.4.0
[85] tibble_3.1.2 AnnotationDbi_1.54.1 memoise_2.0.0 IRanges_2.26.0
[89] ellipsis_0.3.2
Java version:
java version "1.8.0_291"
Java(TM) SE Runtime Environment (build 1.8.0_291-b10)
Java HotSpot(TM) 64-Bit Server VM (build 25.291-b10, mixed mode)
## machine 2
> sessionInfo()
R version 4.0.4 (2021-02-15)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.1 LTS
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0
LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=en_US.UTF-8
[9] LC_ADDRESS=en_US.UTF-8 LC_TELEPHONE=en_US.UTF-8
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods
[7] base
other attached packages:
[1] Matrix_1.3-2 kableExtra_1.3.4 forcats_0.5.1
[4] stringr_1.4.0 dplyr_1.0.7 purrr_0.3.4
[7] readr_1.4.0 tidyr_1.1.3 tibble_3.1.2
[10] ggplot2_3.3.5 tidyverse_1.3.1 MASS_7.3-53.1
loaded via a namespace (and not attached):
[1] tidyselect_1.1.1 xfun_0.24 rJava_1.0-4
[4] lattice_0.20-41 haven_2.4.1 colorspace_2.0-2
[7] vctrs_0.3.8 generics_0.1.0 htmltools_0.5.1.1
[10] viridisLite_0.4.0 utf8_1.2.1 rlang_0.4.11
[13] pillar_1.6.1 glue_1.4.2 withr_2.4.2
[16] DBI_1.1.1 BridgeDbR_2.0.2 dbplyr_2.1.1
[19] modelr_0.1.8 readxl_1.3.1 lifecycle_1.0.0
[22] munsell_0.5.0 gtable_0.3.0 cellranger_1.1.0
[25] rvest_1.0.0 htmlwidgets_1.5.3 evaluate_0.14
[28] knitr_1.33 curl_4.3.2 fansi_0.5.0
[31] broom_0.7.8 Rcpp_1.0.7 BiocManager_1.30.16
[34] scales_1.1.1 backports_1.2.1 webshot_0.5.2
[37] jsonlite_1.7.2 fs_1.5.0 hms_1.1.0
[40] digest_0.6.27 stringi_1.6.2 grid_4.0.4
[43] cli_3.0.0 tools_4.0.4 magrittr_2.0.1
[46] crayon_1.4.1 pkgconfig_2.0.3 ellipsis_0.3.2
[49] xml2_1.3.2 reprex_2.0.0 lubridate_1.7.10
[52] assertthat_0.2.1 rmarkdown_2.9 httr_1.4.2
[55] rstudioapi_0.13 R6_2.5.0 compiler_4.0.4
Java version:
openjdk version "11.0.11" 2021-04-20
OpenJDK Runtime Environment (build 11.0.11+9-Ubuntu-0ubuntu2.20.04)
OpenJDK 64-Bit Server VM (build 11.0.11+9-Ubuntu-0ubuntu2.20.04, mixed mode, sharing)
In addition to the above, I then tried map() for mapping an individual identifier. It didn't throw this error, but also returned nothing:
> BridgeDbR::map(mapper = mapper, source = "L", identifier = "885041", target = "H")
[1] source identifier
<0 rows> (or 0-length row.names)
I can't find out the cause of this myself. Any help will be very much appreciated.
I was trying to map gene identifiers from Entrez gene ID to HGNC symbols using the following code:
The
mapsfunction threwjava.lang.NoSuchMethodError. Traceback info:I tested this on two separate machines, one running R version 4.0.4 with Bioconductor 3.12 and the other running R 4.1.0 with Bioconductor 3.13.
maps()threw the same error on both machines. Oddly, this code snippet worked back in February with no issues. I'm not sure if this error has to do with the Java version. As for thesessioninfo()and the Java versions of the two machines I tested on:Java version:
Java version:
In addition to the above, I then tried
map()for mapping an individual identifier. It didn't throw this error, but also returned nothing:I can't find out the cause of this myself. Any help will be very much appreciated.